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Staphylococcal Enterotoxin M Exhibits Thrombin-like Enzymatic Activity
- Qian Huang,
- Shuang-Hua Luo,
- Wan-Fan Tian,
- Jun-Ni Tang and
- Ji Liu
To express and purify staphylococcal enterotoxin M (SEM) using immobilized metal affinity chromatography (IMAC), a signal peptide-truncated (ΔNsp) wild-type SEM (SEMWT) was N-terminally fused in pET-28a(+) to a polyhistidine tag (His6×-)...