Search Results (1,461)

Recognizing human actions from static images in complex industrial environments remains challenging due to insufficient feature representation and high computational complexity. This issue is particularly critical in power-grid safety monitoring, where improper worker postures (e.g., bending, climbing, falling) can lead to severe accidents and personal injuries, necessitating automated monitoring systems that operate reliably on resource-constrained edge devices. This study proposes a bio-inspired lightweight recognition framework that integrates an improved YOLO-Pose model with a gated recurrent unit (GRU) network. The scientific motivation is grounded in the observation that the human musculoskeletal system achieves highly efficient motion perception through three key mechanisms: hierarchical muscle coordination providing intrinsic rotation invariance, proprioceptive feedback enabling real-time error correction, and selective neural gating reducing redundant information transmission. These biological principles directly inspire our technical contributions: polar-coordinate encoding provides rotation invariance, three-stage filtering mimics proprioceptive feedback, and GRU gating mirrors selective information propagation. Unlike prior approaches that treat pose-based action recognition as a generic computer vision problem, this work explicitly incorporates anatomical structural constraints into the computational pipeline. The framework addresses three research gaps: (1) existing methods lack biomechanically derived invariance properties; (2) GCN-based approaches use fixed topologies that fail to adapt to occlusion patterns; (3) the trade-off between model complexity and accuracy remains unsatisfactory for edge deployment. Experiments on the self-constructed SKPose dataset demonstrate that the proposed method achieves 95.04% accuracy, outperforming ST-GCN by 3.67 percentage points and 2s-AGCN by 1.94 percentage points, with an inference speed of 48 FPS on 8.7 M parameters in underground power-grid environments and provides practical support for biomimetic perception systems and industrial safety monitoring. Full article

Korean native black goat is commonly consumed as thermally processed extracts and is widely perceived to exhibit health-promoting properties; however, the contribution of intrinsic muscle proteins to these functional characteristics remains unclear. This study evaluated the effects of thermal processing on the antioxidant activity of myofibrillar and sarcoplasmic protein fractions using a screening-based approach. Protein fractions were extracted and subjected to various thermal conditions (60–121 °C), followed by analyses of extractable protein, α-amino group content, and antioxidant activities, including 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, hydroxyl radical scavenging, iron-chelating ability, and ferric reducing antioxidant power (FRAP). Thermal processing increased α-amino group content, particularly in the myofibrillar fraction, indicating enhanced protein degradation. Antioxidant activities improved with increasing temperature, with myofibrillar proteins showing stronger activity than sarcoplasmic proteins. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) indicated fragmentation in myofibrillar proteins and aggregation in sarcoplasmic proteins. Cluster analysis supported fraction-dependent differences in antioxidant responses. These findings suggest that thermal processing enhances antioxidant activity in a protein fraction-dependent manner and provide practical insights for optimizing processing conditions of Korean native black goat extracts. Full article

The sources of natural lycopene are diverse, and lycopene from different sources may have differences in functional characteristics and bioavailability. In this study, lycopene was extracted from tomatoes, cherry tomatoes, red guavas, carrots, and watermelons by ultrasonic-assisted extraction, and the structures were characterized. The differences in their in vitro and in vivo antioxidant capacities and anti-inflammatory capacity in vivo were compared. The results showed that under the extraction conditions of this experiment (sample: ethyl acetate: 1:5 m/v, 40 °C, 600 W, 40 kHz, 30 min), lycopene (primarily all-trans structure) from different sources could be effectively extracted from the above five raw materials. The concentration of lycopene extracted from the four samples except tomatoes (14.03 ± 1.08 mg/100 g fresh weight (FW)) was about 30 mg/100 g FW. The analysis of the in vitro antioxidant capacity of lycopene from five different sources showed that the 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), diphenyl-1-picrylhydrazyl (DPPH) scavenging rates and ferric reducing antioxidant power (FRAP) concentration of the red guava lycopene-rich sample were significantly higher than those of the other four sources of lycopene. Based on the in vitro performance of lycopene from five sources, further in vivo experiments (using only the tomato and red guava groups) also found that compared with lycopene from tomatoes, lycopene-rich extract from red guavas could significantly increase the antioxidant enzyme activities and total antioxidant capacity in the serum, liver and gastrocnemius muscle (GAS) of mice; reduce the malondialdehyde (MDA) concentration; and also increase the expression of antioxidant-related genes (GPx, CAT, SOD1, etc.) in the liver and GAS of mice by regulating the Nrf-2/keap1 signaling pathway. In addition, mice in the guava-derived lycopene-rich group had lower serum levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). In summary, these results indicated that the lycopene-rich extract derived from red guava demonstrated higher antioxidant activity both in vitro and in vivo as well as enhanced anti-inflammatory capabilities within the body, providing an important reference for its application in the food industry and functional foods. Full article

Background/Objectives: Low-carbohydrate (LCD) and ketogenic diets (KD) are increasingly adopted by athletes due to their ability to enhance fat oxidation and induce metabolic adaptations. While their effects on aerobic power and capacity have been widely investigated, their influence on anaerobic performance remains unclear. Given the strong dependence of high-intensity exercise on glycolytic metabolism and muscle glycogen availability, carbohydrate restriction may have significant implications for short-duration maximal efforts and repeated high-intensity exercise. Therefore, this systematic review and meta-analysis aimed to evaluate the effects of LCD and KD on anaerobic performance outcomes in trained athletes. Methods: A comprehensive search of five electronic databases (PubMed, SCOPUS, Web of Science, SPORTDiscus, and Cochrane Central Register of Controlled Trials) identified 13 unique studies (yielding 15 study-level entries across three anaerobic performance domains) meeting comprehensive inclusion criteria. Individual study sample sizes ranged from n = 5 to n = 65 participants, reflecting substantial inter-study variability that should be considered when interpreting pooled estimates. Outcomes included peak and mean power output, repeated sprint performance, blood lactate responses, and markers of substrate utilization. Study quality was assessed using the Newcastle–Ottawa Scale, and meta-analyses were performed using random-effects models where appropriate. Results: Overall, the effects of carbohydrate-restricted diets on anaerobic performance were domain-specific. Some studies reported maintained or slightly improved peak power during single maximal efforts, while others showed no effect. Impairments were more consistently observed in repeated high-intensity exercise. Repeated sprint performance was impaired in several studies, likely reflecting reduced muscle glycogen availability and limited glycolytic ATP production. Carbohydrate restriction consistently increased fat oxidation and was associated with lower blood lactate concentrations during high-intensity exercise. Random-effects meta-analyses yielded domain-specific pooled effect sizes: maintained-to-slightly-improved anaerobic power output (Cohen’s d = +0.29; 95% CI: −0.08 to +0.66), modestly impaired repeated sprint ability (d = −0.33; 95% CI: −0.80 to +0.14), and a large, consistent reduction in blood lactate concentration (d = −0.89; 95% CI: −1.20 to −0.58). Given substantial between-study heterogeneity in intervention durations (2 days to 12 weeks), dietary composition, athlete populations, and outcome measures (1RM, Wingate, CMJ within the power domain; varied protocols within the RSA and lactate domains), these pooled estimates should be interpreted as exploratory rather than confirmatory. Conclusions: LCD and KD appear to have domain-specific effects on anaerobic performance in trained athletes. Although single, short-duration efforts may be preserved in some contexts, repeated, high-intensity performance appears to be more susceptible to impairment. These findings highlight the importance of aligning dietary strategies with the metabolic demands of training and competition. Full article

Background: Prehabilitation aims to optimise patients before cardiac procedures through interventions including exercise training, respiratory conditioning, nutritional support, psychological preparation and multimodal lifestyle programmes. Evidence from systematic reviews and meta-analyses is increasing but remains heterogeneous due to variation in intervention design, patient populations and overlap of primary studies. Methods: We conducted an umbrella review of 17 systematic reviews and meta-analyses evaluating prehabilitation prior to cardiac surgery and structural heart interventions in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Methodological quality of included reviews was assessed using A Measurement Tool to Assess Systematic Reviews 2 (AMSTAR 2). Outcomes of interest were postoperative pneumonia, hospital length of stay (LOS), and mortality. Results: Across pooled analyses, the most consistent finding was a reduction in postoperative pneumonia, particularly in studies incorporating inspiratory muscle training (IMT), with relative risk reductions of approximately 55–62%, corresponding to a modest absolute risk reduction. Reductions in hospital LOS were also reported, although effect sizes were smaller and more variable. In contrast, no consistent reduction in short-term mortality was demonstrated, likely reflecting low event rates. The evidence base was limited by substantial overlap between reviews and predominantly low or critically low methodological quality. Conclusions: Prehabilitation, particularly when incorporating IMT, is consistently associated with a reduction in postoperative pneumonia and may contribute to modest reductions in hospital LOS. However, the evidence base is constrained by heterogeneity, study overlap and low methodological quality. Further high-quality, adequately powered randomised trials are required to define the role of prehabilitation in contemporary cardiac surgical and structural intervention practice. Full article

Gesture recognition based on surface electromyography (sEMG) signals typically involves extracting features from the signals and then incorporating recognition models to increase the accuracy of classification. In this paper, drawing on the properties of sEMG signals and statistical principles, we propose a novel feature extraction method called “multiple mapping”, designed to construct a high-performance representation of sEMG signals. The multiple mapping approach incorporates sequential mappings, including the sliding average power, lg (base-10 logarithm) mapping, a linear compression principle, and a sigmoid normalization function. The sliding average power captures the intensity variations in sEMG signals across various channels, allowing differentiation between the activity levels of distinct muscle groups. lg mapping adjusts the distribution of the sEMG signals to improve their uniformity, enhancing feature stability and facilitating comparisons. The linear compression and sigmoid normalization emphasize the signals’ central characteristics while compressing the extremes at both ends. Then, the sEMG signals obtained from multiple mapping are transformed into Sem grayscale maps, which are subsequently processed using the deep neural network ResNet50 for gesture recognition. Extensive experiments on three public datasets were conducted, and the average recognition accuracy was 95.26%, 90.81%, and 96.72%, respectively, while it was 96.8% in self-collected recognition tasks. The results demonstrate that the multiple mapping method significantly improves the feature extraction performance for sEMG-based gesture recognition, offering a promising direction for applications in prosthetic gesture control and muscle–computer interaction. Full article

Background/Objectives: This study evaluated carotid baroreflex sensitivity (cBRS) during graded exercise tests to exhaustion in healthy individuals. It aimed to elucidate whether the augmented blood pressure response during heavy- and maximal-intensity dynamic exercise alters carotid baroreflex control of heart rate and contributes to exercise intolerance. Methods: Thirteen healthy males (age 33 ± 2 yrs, body mass 74.6 ± 2.4 kg, and $\dot{V}$O_2max 54.12 ± 1.88 mL·kg⁻¹·min⁻¹) performed a 4 min constant-load cycling exercise at low—(30% PPO), moderate—(60% PPO), high—(80% PPO), and maximal—(100% PPO) intensity, in two experimental conditions: (a) with unrestricted muscle blood flow (no-BFR) and (b) with partial muscle blood flow restriction (BFR). Results: A significant decline in cBRS was observed during the graded maximal exercise test compared to baseline (p < 0.001), accompanied by an upward and rightward relocation of the linear relationship between systolic blood pressure (SBP) and heart rate (HR). However, the magnitude of cBRS reduction was attenuated towards maximum exercise. Application of BFR during exercise exaggerated the blood pressure rise (p < 0.01), the perceptual response (p < 0.001), the exercise-induced cBRS reduction (p < 0.001), and induced a further relocation of the SBP-HR relationship. Additionally, BFR limited the HR increase and resulted in reduced exercise performance compared to the no-BFR condition. Conclusions: These findings suggest that the pronounced increase in blood pressure during heavy- and maximal-intensity exercise may limit further increases in heart rate through arterial baroreflex activation. This may contribute to reduced exercise tolerance, as evidenced by the lower peak power output and attenuated maximal heart rate observed in muscle BFR condition. Full article

Background: Chronic low back pain (CLBP) and depression often co-occur. This study compared a fascia-focused physiotherapeutic program with a conventional physiotherapeutic and relaxation-based program in psychosomatic inpatients with CLBP and comorbid depression. Methods: In this exploratory quasi-randomized study, 41 inpatients were allocated to a fascia-focused intervention group (n = 23) or a conventional active control group (n = 18). Over six weeks, the intervention group received Bowen therapy and fascia circuit training, whereas the control group received progressive muscle relaxation and strength circuit training. Outcomes were assessed at baseline and after rehabilitation. NRS pain intensity and BDI-II depressive symptom severity were the main clinically relevant outcomes; spinal function, tissue stiffness, pressure pain threshold, and craniovertebral angle were secondary or exploratory outcomes. Results: Both groups improved over time in pain intensity and depressive symptom severity. NRS scores decreased by 3.21 ± 2.61 points in the fascia-focused group and by 2.17 ± 2.34 points in the control group; BDI-II scores decreased by 9.30 ± 13.42 and 7.22 ± 8.57 points, respectively. Repeated-measures ANOVA confirmed significant time effects for NRS and BDI-II, with no significant group differences or time × group interactions. Significant time effects were also observed for thoracic tissue stiffness, lumbar and pelvic posture, thoracic and lumbar mobility, and pelvic stability. Conclusions: Fascia-focused and conventional physiotherapy showed similar observed effects in this exploratory quasi-randomized study. The absence of significant between-group differences should be interpreted cautiously because the study was not designed or powered to establish formal equivalence. Full article

(1) Background: This study aims to investigate the differences in body composition, muscle strength, and power between young karate athletes from different competitive disciplines. (2) Methods: The study sample consisted of 131 participants (69 boys and 62 girls) aged 10–14 years divided into three groups: kata (n = 48), kumite (n = 40), and athletes competing in both disciplines (n = 43). The set of variables included 1 anthropometric variable, 6 variables for assessing body composition (InBody 270), 8 variables for assessing handgrip strength (handgrip strength test, Fmax, RFD, absolute and relative, both hands), and 3 variables for assessing lower limb muscle power (force plate, CMJ height and power). Of the statistic analysis, MANOVA and ANOVA, along with MANCOVA and ANCOVA were performed. (3) Results: The adjusted results revealed significant general differences between groups (from p = 0.005 to p = 0.009). Regarding body composition, kumite athletes are taller, have greater body mass, body water, proteins, minerals, and muscle mass content than kata athletes and athletes specialized in both disciplines (from p = 0.002 to p = 0.045). The young karate athletes specialized in kumite competition exhibit higher levels of absolute handgrip muscle strength, rate of force development, and absolute lower limb muscle power than kata athletes and athletes specialized in both disciplines (from p = 0.002 to p = 0.041). There were no significant differences in any measured parameters between kata athletes and young karate athletes specialised in both disciplines. (4) Conclusions: The results are associated with higher values of body composition, muscle strength, and power in kumite athletes compared to kata athletes and athletes competing in both disciplines. Full article

Background/Objectives: Peripheral(-muscle) oxygenation assessed with near-infrared spectroscopy might serve as an early marker of infection/inflammation; however, evidence of its clinical relevance is lacking so far. This study aimed to develop a peripheral(-muscle) oxygenation and perfusion score (POP-Score) using the peripheral(-muscle) tissue oxygenation index (pTOI) combined with non-invasive monitoring parameters within six hours after birth. The POP-Score was designed to explore associations with elevated C-reactive protein (CRP), as an early infection/inflammation marker, in term and late-preterm neonates. Methods: Secondary outcome parameters from a prospective observational study were analysed. Included neonates weighed ≥2000 g with respiratory distress, excluding those with umbilical artery pH < 7.20. Neonates with CRP ≥ 20 mg/L were 1:4-matched to those with CRP < 20 mg/L by gestational age (±2 weeks). For pTOI measurements, a sensor was placed for a duration of 30 s, followed by four further reapplications of the sensor, using the NIRO200NX within the first six hours after birth. The POP-Score was established using the following formula: (pTOI [%] × subcutaneous fat layer thickness [cm] × heart rate [bpm])/(arterial oxygen saturation [%] × systolic blood pressure [mmHg]). POP-Score was correlated with the highest CRP within 48 h. Results: Thirty neonates were included (median gestational age: 39.1 weeks [CRP < 20 mg/L group] vs. 37.3 weeks [CRP ≥ 20 mg/L group], p = 0.299; median birth weight: 3561 g vs. 3260 g, p = 0.058, respectively). Median POP-Scores were significantly different: 1.11 (CRP ≥ 20 mg/L) vs. 0.85 (CRP < 20 mg/L), p < 0.001. POP-Score correlated positively with CRP (r = 0.341; p = 0.070). In this small exploratory cohort, a POP-Score cut-off of 1.00 was associated with CRP ≥ 20 mg/L (100% sensitivity and 87% specificity); however, these estimates are uncertain due to the limited sample size. Conclusions: This study is the first to describe a new score for peripheral(-muscle) oxygenation and perfusion (POP-Score), which may represent a potential approach for early, non-invasive assessment but requires validation in adequately powered studies before any clinical application. Trial Registration: Clinicaltrials.gov, Trial registration number: NCT04818762, Date of Registration: 26 March 2021. Full article

Background/Objectives: Handgrip strength (HGS) is a widely used indicator of upper-limb muscular strength and a practical proxy for neuromuscular performance across both clinical and athletic contexts. Although HGS is heritable, evidence supporting specific genetic contributors in elite athletes remains limited. Thus, the present study investigated the associations of two functional polymorphisms, BDNF rs6265 (p.Val66Met) and ACTN3 rs1815739 (p.R577X), with HGS performance in elite wrestlers, integrating neuromuscular and muscle fiber-related biological pathways. Methods: The present study included 613 subjects (56 elite male wrestlers (mean age: 22.35 ± 5.34 years; training experience: 13.40 ± 3.85 years) and 557 healthy individuals drawn from a public database). HGS measurements were performed using a digital hand dynamometer. Results: Genotyping was performed on DNA extracted from peripheral blood using a high-density single nucleotide polymorphism (SNP) array. Neither BDNF rs6265 nor ACTN3 rs1815739 was significantly associated with HGS in elite wrestlers (p > 0.05), and effect estimates were negligible. In addition, ACTN3 rs1815739 genotype and allele frequencies were comparable between wrestlers and the reference population, indicating no enrichment of this variant in the elite cohort. In this sample of elite male wrestlers, BDNF rs6265 and ACTN3 rs1815739 were not associated with HGS, and ACTN3 rs1815739 was not enriched relative to a national reference population. Conclusions: These findings suggest no detectable effects of single candidate variants on HGS under the current study design in highly trained athletes; however, this interpretation should be made cautiously given cohort-specific limitations and does not preclude their potential contribution within the broader polygenic architecture of strength-related traits. Future research employing larger, well-powered, and multi-cohort designs and polygenic approaches is warranted to further elucidate the genetic basis of strength phenotypes. Full article

Background/Objectives: Radical cystectomy with urinary diversion is the standard treatment for patients with muscle-invasive bladder cancer, which represents a complex, preference-sensitive decision about the diversion options. To facilitate patient-clinician shared decision-making, we developed and evaluated the feasibility and acceptability of the Personal Patient Profile–Bladder Cancer (P3-BC), a web-based decision support system for patients considering urinary diversion options. Materials and Methods: We employed an iterative development approach following an established framework, including qualitative assessments of radical cystectomy patients’ (n = 30) needs to inform content development, followed by usability testing with 10 key stakeholders. We then conducted a feasibility study with patients newly diagnosed with bladder cancer undergoing cystectomy. Feasibility was assessed through P3-BC completion rates among patients randomized to the intervention and study retention rates. Acceptability was measured using a program-based 12-item questionnaire integrating the six-item Acceptability E-Scale. Secondary outcomes included decisional conflict, shared decision-making, psychological distress, and decisional regret. Results: Of 114 eligible patients, 24 provided consent (21% consent rate), and 15 were randomized to receive the P3-BC intervention (n = 10) or usual care (n = 5). Retention was 93% at 1 month and 73% at 3 months. Among intervention participants, P3-BC achieved high feasibility (100% accessed the program) and acceptability, with 86% reporting overall satisfaction and 86% reporting ease of use. No statistically significant between-group differences were observed in secondary outcomes. Although the study was not powered to examine group differences, numerical trends suggest improvements in decisional conflict and symptom burden over time, particularly in the intervention group. Conclusions: P3-BC demonstrated feasibility and acceptability as a web-based decision support intervention for patients with bladder cancer considering cystectomy and urinary diversion. Primary barriers to enrollment included treatment burden, limited diagnosis-treatment time, and technology concerns. Findings support progression to an adequately powered randomized controlled trial to evaluate clinical effectiveness. Full article

Background/Objectives: This study examined the effects of an eight-week plyometric training programme on jump biomechanics, field-based jump performance, and repeated-jump endurance in 13–14-year-old volleyball players, and explored training-induced changes. Methods: A pre–post controlled experimental design was used. Thirty male volleyball players aged 13–14 years were assigned to an experimental group (EG, n = 15) or a control group (CG, n = 15). The EG completed a structured plyometric programme three times per week for eight weeks in addition to regular volleyball training, whereas the CG continued usual practice. Biomechanical variables were assessed during the Repeated Countermovement Jump test using the BTS Bioengineering G-Walk inertial measurement system, together with field-based jump and repeated-jump endurance tests. Outcomes were analysed using two-way mixed ANOVA (Group × Time). Δ-based correlations were examined using Pearson and Spearman coefficients with false discovery rate correction. Results: Significant Group × Time interaction effects were found for all analysed RCMJ variables (all p < 0.001). Significant interaction effects were also observed for all field-based jump and repeated-jump endurance outcomes (all p ≤ 0.025). The EG showed greater improvements over time than the CG across the principal biomechanical, practical, and endurance-related indicators. Correlation analysis revealed strong associations among the principal RCMJ variables and selected links with field-based and endurance measures. Conclusions: A structured plyometric programme may improve jump biomechanics, jump performance, and repeated-jump endurance in adolescent volleyball players. These findings should be interpreted cautiously because of baseline differences, the modest sample size, and the IMU-derived nature of force- and power-related estimates. Full article

Background and Objective: Transurethral resection of bladder tumor (TURBT) is the standard for non-muscle-invasive bladder cancer (NMIBC), yet recurrence rates remain high. This study evaluates the safety, tolerability, and efficacy of neoadjuvant intravesical mitomycin C (neoMMC) before TURBT in reducing recurrence and improving surgical outcomes. Methods: This randomized phase III trial enrolled patients with primary or recurrent NMIBC. Participants were randomized 1:1 to a neoadjuvant group receiving two instillations of MMC (day −14 and −7) before TURBT, or a control group undergoing standard TURBT without neoadjuvant treatment. The primary endpoint was 12-month recurrence-free survival (RFS). Secondary endpoints included surgical quality (complete resection, cauterization only, absence of residual tumor) and safety. Exploratory endpoints included histopathologic response and time to recurrence. Key Findings and Limitations: Among 95 patients (48 neoMMC, 47 controls), baseline characteristics were balanced. After a median follow-up of 19.4 months, recurrences occurred in 9 StA and 4 NeoA patients, with one progression to MIBC in the NeoA arm. RFS did not differ significantly between groups at 12 or 18 months. Neoadjuvant MMC was well tolerated, with only grade 1–2 AEs. Exploratory microbiota analyses suggested that neoadjuvant MMC modulated urinary microbial diversity and was associated with a microbiota profile more similar to that observed in non-recurrent patients. Limitations include single-center design and relatively short follow-up. Conclusions and Clinical Implications: Neoadjuvant intravesical MMC before TURBT was feasible and well tolerated in patients with NMIBC, with no unexpected safety signals. In this prematurely terminated and underpowered trial, no significant improvement in RFS was observed. Larger adequately powered studies are needed to clarify the oncologic efficacy of this approach. Full article

The global seafood industry faces persistent challenges related to product quality, safety, and authenticity, driven by complex supply chains, increasing demand, and the perishable nature of aquatic products. Traditional analytical methods often fall short in providing rapid, comprehensive, and non-destructive insights into the intricate biochemical changes occurring in seafood. ¹H Nuclear Magnetic Resonance (¹H NMR) spectroscopy has emerged as a powerful and versatile tool for metabolomics, offering a holistic view of the low-molecular-mass compounds (metabolites) present in biological samples. The present study applied ¹H NMR for chemical fingerprint identification in mullets (Mugil liza) from Brazil. Dorsal muscle samples were taken from the fish during summer, autumn, and winter. The procedure involved freeze-drying the muscle tissue, thereafter extracting polar metabolites using designated solvents (methanol, water, and chloroform), and analyzing them using a 600 MHz spectrometer. As a result, 23 metabolites related to degradation biomarkers, essential metabolites, energy expenditure, and muscle structure were identified. The statistical analysis demonstrated a distinct separation between the geographical origins (RJ vs. SC), mostly influenced by variations in the concentrations of lactate, histidine, threonine, phenylalanine, and ornithine. Factors like fish size and seasonal variations did not markedly affect the overall metabolic profile, underscoring the reliability of these chemicals as stable origin indicators. The Principal Component Analysis identified two distinct groups of metabolites, establishing a profile for each geographical origin. The developed protocol can be applied to the processes of geographical identification. Thus, the ¹H NMR tool was efficient in determining metabolites that can be considered biomarkers in analyses for seafood traceability. Full article