Search Results (65)

Behavioral parent training (BPT) is a front-line psychosocial treatment for childhood ADHD, yet its real-world effectiveness is often constrained by parents’ ability to consistently implement learned strategies. Parental ADHD is a prevalent and mechanistically important factor shaping both parenting behavior and child treatment response. Among parents with ADHD, deficits in executive functioning and emotion regulation, abilities essential for consistent and effective BPT implementation, often interfere with parents’ ability to apply learned strategies. Consequently, parental ADHD predicts reduced in-home skill use and attenuated child treatment gains, positioning it as a potentially critical, treatment-relevant risk factor. This narrative review synthesizes evidence on the intergenerational transmission of ADHD-related impairments, the impact of parental ADHD on parenting practices, and the role of parental ADHD as a moderator of BPT outcomes. We also examine existing approaches to addressing parental ADHD within the context of child BPT, including both pharmacological and psychosocial strategies, and evaluate their implications for parenting and child response. Building on this, we propose an intergenerational reconceptualization of psychosocial care for childhood ADHD in which parental functioning is routinely assessed and supported within BPT. Promising directions include integrating CBT-informed strategies to scaffold parents’ cognitive and regulatory processes, incorporating digital health tools that provide just-in-time guidance at the point of parenting performance, and tailoring BPT emphasis for families affected by multigenerational ADHD. Ultimately, embedding parent-focused supports within BPT may be essential for strengthening treatment impact, durability, and real-world effectiveness for many children and families. Full article

The accumulation of cocoons within brood cells of old combs is a key factor causing a series of negative impacts on bee colonies. Previous studies did not sufficiently address this dynamic nature as the core microenvironment for preimaginal bee development. During this accumulation, the enrichment of potentially harmful microorganisms and chemical substances may pose a latent threat to colony health. This study combined microbiome and metabolomics analyses to systematically investigate the potential colony health risks posed by multi-generational accumulation of cocoons in Apis mellifera combs. The results demonstrated that with the growing number of brood rearing generations, the microbial diversity within the cocoons underwent significant shifts. For the bacterial community within multiple-generation cocoons, the Simpson index exhibited a significant increase, whereas indices including Sobs, Ace, and Chao showed significant decreases (p < 0.05). In the fungal community, the Shannon and Pielou_e indices significantly increased, while the Simpson and Faith_pd indices significantly declined (p < 0.05). Potential pathogens such as Melissococcus and the mycotoxin-producing fungus Wallemia became significantly enriched, reaching alarming relative abundances of 42.70% and 13.52%, respectively, in the multiple-generation cocoons. Metabolomic analysis further revealed the enrichment of 685 differential metabolites, including persistent exogenous pesticides such as cyanazine and pymetrozine, etc. Correlation analysis uncovered a significant positive relationship (r > 0.8) between these pesticide residues and pathogen abundance, indicating interactions between pollutants and pathogens that may exacerbate risks. This study reveals the aggravation of microecological imbalance and chemical pollution load within the cocoons of old combs and therefore provides strong scientific support for risk assessment of comb age in colony health management and offers practical guidance for the sustainable development of beekeeping. Full article

This study investigates the physical and cyber-physical resilience of smart grids with a high share of renewable energy sources (RESs) dominated by permanent magnet synchronous generators (PMSGs). The originality of this work lies in the development and unified evaluation of five integrated control strategies, the PLL with grid following, VSG with grid shaping, VSG+BESS, VSG+STATCOM, and VSG+BESS+STATCOM, implemented within a coherent simulation framework based on Python. Unlike previous works that analyze these methods in isolation, this study provides a comprehensive quantitative comparison of their dynamic characteristics, including frequency root mean square deviation, maximum deviation, and composite resilience index (RI). To extend the analysis beyond static conditions, a multi-generator (multi-PMSG) scenario with heterogeneous inertia constants and variable load profiles is introduced. This dynamic model allows the evaluation of natural inertia diversity and the effects of inter-generator coupling compared to the synthetic inertia emulation provided by VSG-based control. The combined VSG+BESS+STATCOM configuration achieves the highest synthetic resilience, improving frequency and voltage stability by up to 15%, while the multi-PMSG system demonstrates comparable or even higher RI values due to its inherent mechanical inertia and decentralized response behavior. In addition, a cyber-physical scenario is included to evaluate the effect of communication delays and false data injection (FDI) on VSG frequency control. The results show that a communication delay of 50 ms reduces RI by approximately 0.2%, confirming that even minor cyber disturbances can affect synchronization and transient recovery. However, hybrid control architectures with local energy buffering (BESS) show superior resilience under such conditions. The main technical contribution of this work is the establishment of an integrated analytical and simulation framework that enables the joint assessment of synthetic, natural, and cyber-physical resilience in converter-dominated smart grids. This framework provides a unified basis for the analysis of dynamic stability, hybrid control interaction, and the impact of cyber uncertainty, thereby supporting the design of low-inertia, resilient, and secure next-generation power systems. Full article

Natamycin, a polyene macrolide antifungal, has long been used as a food preservative and is the only Food and Drug Administration (FDA)-approved topical treatment for fungal keratitis. While its safety is supported by specific ergosterol interaction and minimal systemic absorption, current research mainly focuses on short-term effects, often overlooking long-term, developmental, and microbiome-related impacts. In food applications, questions remain about cumulative exposure and potential disruptions to gut microbiota. For ophthalmology, advanced delivery methods like nanocarriers and hydrogels enhance drug penetration but may alter pharmacokinetics and pose formulation challenges. Regulatory approvals have historically depended on established safe use and limited toxicological data, emphasizing the need for more systematic evaluations. Zebrafish (Danio rerio) represent a promising yet underutilized model for addressing significant gaps in research, particularly in the realms of microbiome studies, ocular health, developmental processes, and multigenerational effects. When paired with omics technologies, zebrafish facilitate comprehensive system-level mapping of drug-induced outcomes. This review consolidates existing evidence and positions zebrafish as a vital translational link between in vitro assays, mammalian models, and clinical practice. Additionally, it proposes a framework to ensure the effective and scientifically supported use of natamycin in both food and medicinal applications. Full article

Background: Multiple Sclerosis (MS) is a chronic, autoimmune, multifactorial, and complex disorder of the central nervous system (CNS), affecting more than 2 million individuals globally. Genome-wide association studies (GWAS) have explained only a small fraction of its high heritability, highlighting the need for alternative approaches to identify rare genetic variants that contribute to its etiology. To address this, we performed whole-exome sequencing (WES) in a multi-affected family. Methods: WES was performed in a MS multigenerational family comprising two affected sisters, their two healthy brothers, and one affected son. Results: Bioinformatics analysis identified 47 co-segregating rare variants. Three missense variants in genes involved in inflammation, autoimmunity, and demyelinization were identified as the most promising candidates: c.443 C>T, p.Pro148Leu in the RTN4 gene, c.1678 T>G, p.Phe560Val in the JAK2 gene, and c.3449 A>G, p.Tyr1150Cys in the DUOX2 gene. Protein modeling and in silico tools suggest that the three selected variants may have a significant impact on protein function. Conclusions: We identified novel candidate genes for MS in a multiplex family, providing evidence for an oligogenic model of disease susceptibility. Further replication and functional studies are required to validate these preliminary results. Full article

Introduction: Cigarette smoking is unquestionably associated with an increase in morbidity and mortality worldwide, exerting significant adverse effects on respiratory health. The impact of tobacco persists in the epigenome long after smoking cessation. Furthermore, the offspring of smokers may also be affected by the detrimental effects of smoking. Material and methods: The modifications made to the body, such as DNA methylation, histone modification, and regulation by non-coding RNAs, do not change the DNA sequence but can influence gene expression. In respiratory disease, multigenerational effects have been reported in humans, with an increased risk of asthma or COPD and decreased lung function in offspring, despite them not being exposed to smoke. Prenatal nicotine exposure leads to pulmonary pathology that persists across three consecutive generations, supported by animal studies conducted by Rehan et al. Significant advances in high-throughput genomic and epigenomic technologies have enabled the discovery of molecular phenotypes. These either reflect or are influenced by them. Due to the hidden environmental effects and the rise of artificial intelligence (AI) in biomedical research, new predictive models are emerging that not only explain complex data but also enable earlier detection and prevention of smoking-related diseases. In this narrative review, we synthesise the latest research on how smoking affects gene regulation and chromatin structure, emphasising how tobacco can increase vulnerability to multiple diseases. Discussion: For many years, it was widely believed that diseases are solely inherited through genetics. However, recent research in epigenetics has led to a significant realisation: environmental factors play a crucial role in an individual’s life. External influences leave a mark on DNA that can influence future health and offer insights into potential illnesses. In this context, it is possible that in the future, doctors might treat people not as a whole but as individual beings, with personalised medication, tests, and other approaches. Conclusions: The accumulated evidence suggests that exposure to various environmental factors is associated with multigenerational changes in gene expression patterns, which may contribute to increased disease risk. The application of artificial intelligence in this domain is currently a crucial tool for researching potential future health issues in individuals, and it holds a powerful prospect that could transform current medical and scientific practice. Full article

Silver (Ag) is widely released into aquatic environments through industrial and municipal discharges, with concentrations often reaching toxic levels for aquatic organisms. Its further extensive use in antimicrobials, especially during the COVID-19 pandemic, has increased environmental inputs. As Ag⁺ is the most toxic form of Ag, understanding its ecological risks remains critical for environmental regulation and ecosystem protection. Thus, we investigated multigenerational and transgenerational toxicity of Ag⁺ as AgNO₃ on the ecologically important species midge Chironomus riparius using two complementary long-term life-cycle experiments. Experiment 1 simulated exposures with pulsed high environmentally relevant concentrations and recovery phases (nominal 3 µg/L), while Experiment 2 assessed continuous low environmentally relevant concentrations (nominal 0.01, 0.1, 1 and 3 µg/L) across four exposed generations of C. riparius followed by three recovery generations. Endpoints included survival, development, reproduction, growth as well as the population growth rate (PGR). Continuous Ag⁺ exposure produced cumulative increases in mortality and declines in emergence, reduced fertility and eggs per rope, delayed development (especially in females), and progressive reductions in PGR. Notably, adverse effects emerged or intensified over generations and were detectable at very low concentrations: some reproductive and survival endpoints showed significant impairment at the European Union’s environmental quality standard (EU-EQS) level (0.01 µg/L) by the fourth generation, while transgenerational effects persisted at ≥0.1 µg/L. Partial recovery occurred after removal of contamination at the lowest concentrations but not after higher exposures. The present study not only indicates that chronic, low-level Ag⁺ contamination can produce persistent, population-level adverse impacts on C. riparius, but also underscores the necessity for long-term ecological assessments to establish more protective standards and maintain ecosystem stability. Full article

Endocrine disruptors contaminate indoor and outdoor air, water, and food. Besides modifications of the androgen/estrogen balance, endocrine disruptors can alter thyroid function, metabolic balance, immune defenses, and brain development during fetal life, childhood, and adolescence. Among the consequences of fetal exposure to endocrine disruptors, neurobehavioral disorders, particularly psychiatric disorders (for example, schizophrenia and bipolar disorder), attention deficit disorders, and mood disorders, occupy a special place. Therefore, endocrine disruptors are also neuroendocrine disruptors. This review article first summarizes the direct and transgenerational effects of endocrine disruptors. Then, data from a French national cohort of patients whose mothers were treated with synthetic hormones (estrogens and/or progestogens) during their pregnancy(ies) are used to describe the psychiatric disorders developed by children exposed in utero and the multigenerational and potentially transgenerational impacts. Full article

This study evaluates the relationship between language shift and linguistic change in multigenerational immigrant communities, focusing on North American Norwegian (NAmNo) and German heritage varieties. The research synthesizes current findings on how language shift impacts linguistic structures in moribund heritage varieties. Methods include a qualitative review of diachronic studies, comparing data from different periods to assess changes in tense morphology, language mixing, compositional definiteness, possessive placement, verb placement, argument placement, and phoneme variation. Results indicate that the last generation of heritage speakers demonstrates increased linguistic innovation and variation compared to earlier generations. Key findings show that language shift leads to different input quality and quantity, affecting grammatical stability. The study concludes that sociocultural changes, such as verticalization and domain-specific language use, significantly influence heritage language maintenance and loss. These insights contribute to understanding the dynamics of language shift and its role in heritage language change, offering valuable comparative perspectives across different immigrant communities. Full article

Background/Objectives: Little is known about experiences of sleep disturbance in dementia amongst South Asian families, the UK’s biggest minority ethnic group. We aimed to explore their experiences of these alongside translation and preliminary cultural adaptation of an existing effective multicomponent intervention, DREAMS-START. Methods: We interviewed family carers of people living with dementia who had participated in the DREAMS-START (n = 7) trial or other dementia studies (n = 4), conducting reflective thematic analysis. We translated DREAMS-START into Hindi and consulted with Hindi speakers with experience in dementia care, revising iteratively. Results: We identified two overarching themes: (i) the experience of dementia-related sleep disturbance in South Asian families, including the impact of multigenerational living, cultural expectations and practices, and existing relationships; and (ii) considerations for culturally adapting DREAMS-START, including language barriers, linguistic and other changes to peripheral elements to increase engagement and relevance, and culturally competent facilitation of the intervention. Conclusions: Consideration of multigenerational family structures, within-member dynamics, culturally appropriate activities and lack of access to support are important during consultation and intervention. It was thought that cultural adaptation of the intervention in language and facilitator cultural competence, including consideration of the schedule of prayer, would increase relevance and thus community access. We will use this preliminary work to inform future cultural adaptation and testing of the intervention with the intention to widen access for UK-based South Asian families. Full article

Quaternary ammonium compounds (QACs) are a class of chemicals used for their antimicrobial, surfactant, and antistatic properties. QACs are present in many consumer products, and people are regularly exposed to them. We have previously shown reproductive toxicity in mice exposed to the disinfectants alkyl dimethyl benzyl ammonium chloride (ADBAC) and dodecyl dimethyl ammonium chloride (DDAC). To assess the long-term reproductive impacts, a generational reproductive study was conducted. Sperm parameters were determined by CASA and epigenetic enzyme mRNA expression was determined by pathway-focused RT-PCR. Mice ambiently exposed to ADBAC+DDAC exhibited decreases in reproductive indices that persisted through the F1 generation. Male mice (F0) dosed with 120 mg/kg/day of ADBAC+DDAC exhibited decreased sperm concentration and motility that persisted through the F1 generation. Changes in the mRNA expression of chromatin-modifying enzymes in the testes were seen. Two histone acetyltransferases (Hat1 and Kat2b) were upregulated, and one lysine-specific demethylase (Kdm6b) was downregulated in the F0 generation. The DNA methyltransferase Dnmt1 was downregulated in F1 males. These changes in chromatin-modifying enzymes are known to decrease fertility and could be a mechanism for ADBAC+DDAC reproductive toxicity. In all experiments, the F2 generation was similar to the controls, showing multi-generational but not trans-generational epigenetic inheritance. Full article

To investigate the sintering shrinkage behavior of multigeneration, multilayer zirconia materials using geometrical measurements. Seven zirconia CAD/CAM materials were analyzed, comprising two mono-generation zirconia (HTML: Katana Zr, HTML Plus, 3Y-TZP; UTML: Katana Zr, UTML, 5Y-TZP) and five strength-gradient multilayer zirconia (AIDI: optimill 3D PRO Zir; PRIT: Priti multidisc ZrO₂ multicolor; UPCE: Explore Esthetic; ZCPC: IPS e.max ZirCAD Prime; ZYML: Katana YML) materials. Cubes (10 × 10 × 10 mm³) were milled in varying positions within the disks. Geometrical measurements were applied before and after dense sintering using a micrometer screw gauge, light microscopy, as well as surface scans and shrinkages were calculated. Data were analyzed using Kolmogorov–Smirnov, five-way ANOVA followed by the Scheffé post hoc test, and partial eta squared, as well as the Kruskal–Wallis test, including Bonferroni correction (p < 0.05). The highest influence on the shrinkage was exerted by the zirconia material (^η_P² = 0.893, p < 0.001), followed by the test method (^η_P² = 0.175, p < 0.001), while the vertical and horizontal position and measurement point showed no impact on the shrinkage results (p = 0.195–0.763) in the global analysis. Depending on the test method, the pooled shrinkage values of all tested zirconia materials varied between 17.7 and 20.2% for micrometer screw gauge, 17.7 and 20.1% for light microscopy, and 17.8 and 21.1% for surface scan measurements. The shrinkage values measured in the upper, middle, and lower multilayered vertical direction did not differ significantly in the global analysis for the multilayer materials. Therefore, a uniform shrinkage of these strength-gradient multilayer zirconia materials within clinically relevant restorations can be assumed. Full article

Promoting environmental stewardship among youths is crucial for inspiring collaborative, multi-generational actions to tackle long-term environmental challenges. This research study explores the impact of an environmental education (EE) field trip, which highlighted wastewater management and renewable energy technology, on high school students using the revised new ecological paradigm (NEP) scale as a key metric in a pre-post survey, which uses traditionally pro (NEP) and anti (dominant social paradigm, DSP) conservationist statements to measure beliefs towards the environment. When applying the Wilcoxon signed-rank test (null hypothesis t = 0, no change) to the series of environmental stewardship action questions “___ is an extremely important part of protecting the environment”, we identified ten out of the thirteen scale questions to show significant change, all of which were positive. Additionally, the overall impact score was positive and significant (p ≤ 0.05). This finding demonstrates that respondents felt more strongly that these variables played a role in protecting the environment after experiencing the field trip. This suggests that exposure to environmental management intervention strategies utilizing man-made infrastructure and technology may enhance human capability to positively influence the environment and mitigate environmental threats, potentially alleviating concerns about environmental issues. These results suggest that environmental stewardship in youth needs to be reconceptualized in an increasingly STEM-focused world, and a new metric should be developed to assess environmental beliefs. Full article

The deepening operation of the carbon emission trading market has reshaped the cost–benefit structure of the power generation side. In the process of participating in the market quotation, power suppliers not only need to calculate the conventional power generation cost but also need to coordinate the superimposed impact of carbon quota accounting on operating income, which causes the power suppliers a multi-time-scale decision-making collaborative optimization problem under the interaction of the carbon market, power market, and coal market. This paper focuses on the multi-market-coupling decision optimization problem of thermal power suppliers. It proposes a collaborative bidding decision framework based on a multi-agent deep deterministic policy gradient (MADDPG). Firstly, aiming at the time-scale difference of multi-sided market decision making, a decision-making cycle coordination scheme for the carbon–electricity–coal coupling market is proposed. Secondly, upper and lower optimization models for the bidding decision making of power suppliers are constructed. Then, based on the MADDPG algorithm, the multi-generator bidding scenario is simulated to solve the optimal multi-generator bidding strategy in the carbon–electricity–coal coupling market. Finally, the multi-scenario simulation based on the IEEE-5 node system shows that the model can effectively analyze the differential influence of a multi-market structure on the bidding strategy of power suppliers, verifying the superiority of the algorithm in convergence speed and revenue optimization. Full article

This study evaluates the impact of PM2.5 exposure from wood combustion on reproductive health and fetal development using an experimental model in Sprague Dawley rats. The study was conducted in Temuco, Chile, where high levels of air pollution are primarily attributed to residential wood burning. A multigenerational exposure model was implemented using controlled exposure chambers with filtered (FA) and unfiltered (NFA) air. Second-generation (G2) female rats (n = 48) were exposed pregestationally (60 days) and gestationally (23 days) under four conditions: FA/FA, FA/NFA, NFA/FA, and NFA/NFA. PM2.5 concentration and composition were monitored using beta-ray attenuation and X-ray fluorescence spectrometry. Reproductive parameters, ovarian follicle counts, and hormonal levels were assessed via vaginal cytology, histological analysis, and chemiluminescence immunoassays. PM2.5 exposure disrupted estrous cyclicity (p = 0.0001), reduced antral and growing follicles (p = 0.0020; p = 0.0317), and increased post-implantation losses (p = 0.0149). Serum progesterone and estradiol levels were significantly altered (p < 0.05). Despite ovarian disruptions, fertility rates remained unchanged. These findings suggest that chronic exposure to wood smoke-derived PM2.5 adversely affects ovarian function and fetal growth without significantly impairing overall reproductive capacity. This study highlights the need for public health policies to mitigate wood smoke pollution. Full article