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Article

7 Citations

4,090 Views

10 Pages

In Vivo Simultaneous Analysis of Gene Expression by Dual-Color Luciferases in Caenorhabditis elegans

Motomichi Doi,
Megumi Sato and
Yoshihiro Ohmiya

Int. J. Mol. Sci.2021, 22(1), 119;https://doi.org/10.3390/ijms22010119

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24 December 2020

Both fluorescent and luminescent observation are widely used to examine real-time gene expression patterns in living organisms. Several fluuorescent and luminescent proteins with specific optical properties have been developed and applied for simulta...

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Review

24 Citations

7,696 Views

14 Pages

How to Select Firefly Luciferin Analogues for In Vivo Imaging

Ryohei Saito-Moriya,
Jun Nakayama,
Genta Kamiya,
Nobuo Kitada,
Rika Obata,
Shojiro A. Maki and
Hiroshi Aoyama

Int. J. Mol. Sci.2021, 22(4), 1848;https://doi.org/10.3390/ijms22041848

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12 February 2021

Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence en...

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Article

13 Citations

4,492 Views

17 Pages

S-Series Coelenterazine-Driven Combinatorial Bioluminescence Imaging Systems for Mammalian Cells

Genta Kamiya,
Nobuo Kitada,
Tadaomi Furuta,
Takashi Hirano,
Shojiro A. Maki and
Sung-Bae Kim

Int. J. Mol. Sci.2023, 24(2), 1420;https://doi.org/10.3390/ijms24021420

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11 January 2023

A unique combinatorial bioluminescence (BL) imaging system was developed for determining molecular events in mammalian cells with various colors and BL intensity patterns. This imaging system consists of one or multiple reporter luciferases and a ser...

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