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Review

How to Select Firefly Luciferin Analogues for In Vivo Imaging

1
School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo 192-0392, Japan
2
Department of Engineering Science, Graduate School of Informatics and Engineering, The University of Electro-Communications, Tokyo 182-8585, Japan
3
Center for Neuroscience and Biomedical Engineering, The University of Electro-Communications, Tokyo 182-8585, Japan
4
Division of Cellular Signaling, National Cancer Center Research Institute, Tokyo 104-0045, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Eugene S. Vysotski
Int. J. Mol. Sci. 2021, 22(4), 1848; https://doi.org/10.3390/ijms22041848
Received: 22 January 2021 / Revised: 5 February 2021 / Accepted: 9 February 2021 / Published: 12 February 2021
Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models. Many researchers have developed single-color or multicolor bioluminescence systems based on artificial luciferin analogues and/or luciferase mutants, for application in vivo bioluminescence imaging (BLI). In the current review, we focus on the characteristics of firefly BLI technology and discuss the development of luciferin analogues for high-resolution in vivo BLI. In addition, we discuss the novel luciferin analogues TokeOni and seMpai, which show potential as high-sensitivity in vivo BLI reagents. View Full-Text
Keywords: bioluminescence imaging; near-infrared light; luciferin analogue; luciferase; multicolor; high sensitivity bioluminescence imaging; near-infrared light; luciferin analogue; luciferase; multicolor; high sensitivity
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MDPI and ACS Style

Saito-Moriya, R.; Nakayama, J.; Kamiya, G.; Kitada, N.; Obata, R.; Maki, S.A.; Aoyama, H. How to Select Firefly Luciferin Analogues for In Vivo Imaging. Int. J. Mol. Sci. 2021, 22, 1848. https://doi.org/10.3390/ijms22041848

AMA Style

Saito-Moriya R, Nakayama J, Kamiya G, Kitada N, Obata R, Maki SA, Aoyama H. How to Select Firefly Luciferin Analogues for In Vivo Imaging. International Journal of Molecular Sciences. 2021; 22(4):1848. https://doi.org/10.3390/ijms22041848

Chicago/Turabian Style

Saito-Moriya, Ryohei, Jun Nakayama, Genta Kamiya, Nobuo Kitada, Rika Obata, Shojiro A. Maki, and Hiroshi Aoyama. 2021. "How to Select Firefly Luciferin Analogues for In Vivo Imaging" International Journal of Molecular Sciences 22, no. 4: 1848. https://doi.org/10.3390/ijms22041848

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