Search Results (25)

This study assesses sediment toxicity in the historically contaminated Orbetello Lagoon (southern Tuscany) using Paracentrotus lividus embryo development bioassays. Elutriates from 15 sites were analysed for trace metals, organic matter, and ammonium. Despite elevated mercury concentrations, toxicity did not consistently correlate with metal levels. Instead, Principal Component Analysis (PCA) identified ammonium as a key driver of developmental toxicity, suggesting that it significantly influences both biological effects and metal bioavailability. These results demonstrate that ammonium, often overlooked, can confound sediment toxicity assessments and should be integrated into risk evaluation frameworks for coastal systems affected by legacy pollution. Full article

Soil potentially toxic element (PTE) contamination remains a global concern, particularly in rural agricultural regions. This study collected 157 agricultural topsoil samples within a rural area in SW China. Combined with multivariate statistical analysis in the compositional data analysis (CoDa) perspective, the PMF model was applied to identify key contamination sources and quantify their contributions. Potential ecological risk assessment and Monte Carlo simulation were employed to estimate ecological-health risks associated with PTE exposure. The results revealed that the main exceeding PTEs (Mercury—Hg and Cadmium—Cd) are rich in urbanized areas and the GFGP (Grain for Green Program) regions. Source apportionment indicated that soil parent materials constituted the dominant contributor (32.48%), followed by traffic emissions (28.31%), atmospheric deposition (21.48%), and legacy agricultural effects (17.86%). Ecological risk assessment showed that 60.51% of soil samples exhibited higher potential ecological risk (PERI > 150), with moderate-risk areas concentrated in the GFGP regions. The elements Cd and Hg from legacy agricultural effects and atmospheric deposition contributed the most to ecological risk. Health risk assessment demonstrated that most risk indices fell within acceptable ranges for all populations, while only children showed elevated non-carcinogenic risk (THI_max > 1.0). Among PTEs, the element As, mainly from traffic emissions, was identified as a priority control element due to its significant health implications. Geospatial distributions showed significant risk enrichment in the GFGP regions (legacy agricultural areas). These findings present associated risk levels in sustainable agricultural regions, providing valuable data to support soil environmental management in regions requiring urgent intervention worldwide. Full article

This contribution is aimed at analyzing a series of life-prolonging and rejuvenating treatments (bcud len), recipes of ambrosia for immortality (bdud rtsi sman), and yogic and meditative techniques incorporated in the Man ngag bye ba ring bsrel (Ten Million of Quintessential Instructions, The Relics; henceforth Ring bsrel), a medical corpus mainly authored by Zur mkhar ba mNyam nyid rdo rje (1439–1475). The many treads of mNyam nyid rdo rje’s literary production reflect his rNying ma and bKa’ brgyud spiritual legacies: the author systematically elaborated materials drawn from the rNying ma gter ma tradition and complex medico–alchemical practices ascribed to the third Karmapa Rang byung rdo rje (1284–1339) and O rgyan pa rin chen dpal (1230–1309). The key ingredients of the recipes include saxifrage [bdud rtsi ’od ldan; Micranthes melanocentra; Saxifraga melanocentra], purple sage [lug mur; Phlomoides bracteosa], potent substances such as black aconite [bstan dug; Aconitum spicatum;], nightshade [thang phrom; Anisodus spp.; Mandragora caulescens], mercury–sulfide ashes (zla bcud; bcud rgyal), aphrodisiacs containing salamander meat [da byid, gangs sbal; Batrachuporus pinchonii], caterpillar fungus [dByar rtswa dgun ’bu; Cordyceps sinensis], and other substances endowed with hot potencies that can enhance gtum mo practices such as the dwarf rhododendron [da lis; Rhododendron spp.] and compounds containing long peer [pi pi ling; Piper longum], black pepper, [pho bar is; Piper nigrum], ginger, and so forth. The consecrated medicines and the rituals can bestow protection from any sort of diseases, diseases-carrier forces (gdon), and poisoning and lead to spiritual achievements. The analysis of these scriptural materials brings into focus the historical interrelation of diverse traditions that are represented by the heterogeneity of ideas and practices handed down through the Zur medical lineage. Full article

Despite decreasing anthropogenic mercury (Hg) emissions in Europe and the banning and restriction of many persistent organic pollutants (POPs) under the Stockholm Convention, Mediterranean marine mammals still have one of the highest body burdens of persistent pollutants in the world. Moreover, the Mediterranean basin is one of the most sensitive to climate change, with likely changes in the biogeochemical cycle and bioavailability of Hg, primary productivity, and the length and composition of pelagic food webs. The availability of food resources for marine mammals is also affected by widespread overfishing and the increasing number of alien species colonizing the basin. After reporting the most recent findings on the biogeochemical cycle of Hg in the Mediterranean Sea and the physico-chemical and bio-ecological factors determining its exceptional bioaccumulation in odontocetes, this review discusses possible future changes in the bioavailability of the metal. Recent ocean–atmosphere–land models predict that in mid-latitude seas, water warming (which in the Mediterranean is 20% faster than the global average) is likely to decrease the solubility of Hg and favor the escape of the metal to the atmosphere. However, the basin has been affected for thousands of years by natural and anthropogenic inputs of metals and climate change with sea level rise (3.6 ± 0.3 mm year⁻¹ in the last two decades), and the frequency of extreme weather events will likely remobilize a large amount of legacy Hg from soils, riverine, and coastal sediments. Moreover, possible changes in pelagic food webs and food availability could determine dietary shifts and lower growth rates in Mediterranean cetaceans, increasing their Hg body burden. Although, in adulthood, many marine mammals have evolved the ability to detoxify monomethylmercury (MMHg) and store the metal in the liver and other organs as insoluble HgSe crystals, in Mediterranean populations more exposed to the metal, this process can deplete the biological pool of Se, increasing their susceptibility to infectious diseases and autoimmune disorders. Mediterranean mammals are also among the most exposed in the world to legacy POPs, micro- and nanoplastics, and contaminants of emerging interest. Concomitant exposure to these synthetic chemicals may pose a much more serious threat than the Se depletion. Unfortunately, as shown by the literature data summarized in this review, the most exposed populations are those living in the NW basin, the main feeding and reproductive area for most Mediterranean cetaceans, declared a sanctuary for their protection since 2002. Thus, while emphasizing the adoption of all available approaches to mitigate anthropogenic pressure with fishing and maritime traffic, it is recommended to direct future research efforts towards the assessment of possible biological effects, at the individual and population levels, of chronic and simultaneous exposure to Hg, legacy POPs, contaminants of emerging interest, and microplastics. Full article

The mercury cell manufacturing process, which has been extensively used in chlor-alkali plants to produce chlorine and caustic soda by electrolysis, represents a major source of Hg environmental pollution. At Saline di Volterra (Tuscany, Italy), solution mining by pumping water into halite deposits was applied to produce brines for a mercury-cell chlor-alkali plant. The Hg-contaminated, exhausted brines were pumped back at depth into the rock salt field in order to renew the available resources. Activities ceased in 1994, following the leakage dispersion of highly contaminated Hg(0)-bearing brines into the environment. The mercury content in the soil, measured during a survey conducted in 2000, reached 334 mg/kg, highlighting diffuse contamination in the floodplain. By 2009, the Hg concentration had generally decreased and was mostly confined to the topsoil layer. In order to evaluate the present Hg soil pollution, a geochemical survey was carried out in 2023, almost thirty years after the contamination event. The obtained data indicated the occurrence of legacy Hg, which reached 25.5 mg/kg in some soil samples. Speciation analysis for the most contaminated soil revealed that Hg(0) represented about 17.3% of the total Hg and that water-soluble and organic Hg fractions were negligible. These results suggest that the originally released metallic mercury has volatilized and likely oxidized, becoming practically immobile in the soil. A risk assessment, performed by applying Hg speciation analysis, indicated that the mercury in the soil does not carry a risk of non-cancerous effects for different exposure routes in case of subsequent use of the site and that the formerly contaminated area can now be converted into a leisure area. Full article

The Sea of Azov, an inland shelf sea bounding Ukraine and Russia, experiences the effects of ongoing and legacy pollution. One of the main contaminants of concern is the heavy metal mercury (Hg), which is emitted from the regional coal industry, former Hg refineries, and the historic use of mercury-containing pesticides. The aquatic biome acts both as a major sink and source in this cycle, thus meriting an examination of its environmental fate. This study collated existing Hg data for the SoA and the adjacent region to estimate current Hg influxes and cycling in the ecosystem. The mercury-specific model “Hg Environmental Ratios Multimedia Ecosystem Sources” (HERMES), originally developed for Canadian freshwater lakes, was used to estimate anthropogenic emissions to the sea and regional atmospheric Hg concentrations. The computed water and sediment concentrations (6.8 ng/L and 55.7 ng/g dw, respectively) approximate the reported literature values. The ongoing military conflict will increase environmental pollution in the region, thus further intensifying the existing (legacy) anthropogenic pressures. The results of this study provide a first insight into the environmental Hg cycle of the Sea of Azov ecosystem and underline the need for further emission control and remediation efforts to safeguard environmental quality. Full article

The introduction of mercury (Hg) into the environment by anthropogenic activities has resulted in negative implications for ecosystem functions and human health. Unlike the legacy of huge environmental pollution left by historic gold rushes in several developed countries, gold-rich nations in the developing world are currently witnessing what could qualify as a “new gold rush”, conducted primarily by small-scale mining operators and characterized by the use of metallic Hg (Hg⁰) in the amalgamation process to extract gold from crude ores. Once introduced into the environment, Hg⁰ can undergo biogeochemical transformations to produce Hg species such as methyl-Hg, with well-established adverse impacts on living organisms. This review summarizes published data on both historical and recent trends of the use of Hg⁰ in artisanal gold mining (AGM) on a global scale and emphasizes the impacts of AGM on the environment. To achieve this, we used citations from research conducted in North and South America, Europe, Asia, Africa, Australia, and New Zealand, obtained from several search engines and databases. Our findings show that, in addition to the well-known environmental and human health adverse effects of gold mining with Hg⁰, gold extraction by the Hg amalgamation technique is boosting the economy in parts of Africa, South America, and Asia. Unfortunately, this appealing aspect of AGM may not be easily halted, pending the creation of alternative employment. Therefore, there is a clear need for the development of safe and affordable gold extraction and purification technologies. Ultimately, the growth of this specific economic sector should be regulated to help protect both the environment and human health. Information compiled in this review should help to (i) improve the mapping of AGM-impacted soil and aquatic systems on a global scale and (ii) stimulate discussions and research on how to take down current barriers to the development and implementation of safe AGM methods. Full article

The Toce River (Northern Italy) is characterized by legacy contamination of dichloro-diphenyl-trichloroethane (DDT), mercury, and arsenic deriving from an industrial plant active between ca. 1915 and 1996. Chironomidae taxa assemblages and sediments were collected in 2014 and 2019 upstream and downstream of the industrial area to analyze species responses to toxic substances in a river stretch with relatively uniform natural (i.e., hydro-morphological) characteristics. A total of 32 chironomid taxa were identified. Sediment concentrations reached levels potentially toxic for benthic invertebrates: 15.7 µg kg⁻¹ 1% organic carbon for DDT, 197 µg kg⁻¹ dry weight (d.w.) for Hg, and 55.7 mg kg⁻¹ d.w. for As. Canonical Correspondence Analysis (CCA) revealed a predominant seasonal gradient, followed by an upstream-downstream gradient. Partial CCA indicated that 5.2% of the total variation was associated with sediment contamination. Self-Organizing Maps (SOMs) were used to represent species responses to toxicants. Most species appeared to be tolerant, e.g., Chironomus riparius, Micropsectra atrofasciata, Conchapelopia pallidula, and Polypedilum spp. Sensitivity to contaminants was observed in only a few species: Diamesa spp., Sympotthastia spinifera, and Prodiamesa olivacea to DDT; Potthastia longimanus to Hg; Odontomesa fulva and Microtendipes pedellus to As. The chironomid community was characterized in presence of contamination levels commonly observed in freshwater ecosystems. Full article

Our study addressed mercury contamination hotspots that originated from Chemical Factory Marktredwitz, Germany. The factory was abandoned in 1985 but its legacy has been persistently endangering the river ecosystem of the Ohře River, a Labe (Elbe) River tributary in the Czech Republic. We identified the timing for the peak contamination of fine sediments entering the Skalka Reservoir located on the Ohře River downstream of the Czech German boundary. Age constraints for the reservoir sediments were obtained using gamma spectrometry analyses of ¹³⁷Cs and unsupported (excess) ²¹⁰Pb. We also summarised historical and current Hg concentrations in suspended particulate matter in the Kössein–Röslau–Ohře river system and recent Hg concentrations in aquatic plants. Secondary contamination and its transfer to the Czech stretch of the Ohře River and the Skalka Reservoir through severely contaminated suspended material peaked during the period of factory closure and the start of remediation. The Hg contamination import to the Czech Republic is not likely to improve if the river is left without traditional management of bank reinforcement. This case study highlights a gap in safety regulations for the management of severely contaminated rivers and demonstrates the need to consider the role of historical contamination in river ‘renaturation’. Full article

The Mediterranean Sea is characterized by a marked mercury (Hg) geochemical anomaly, arising in part from large Hg deposits. Mercury mass loads discharged from the Monte Amiata mining district (Central Italy) to the Mediterranean Sea through the Paglia–Tiber River system were estimated. Data from two seasons showed that up to 40 kg year⁻¹ of Hg are drained to Tiber River and finally to the Mediterranean Sea. The mercury mass loads varied in different seasons, from 3 mg day⁻¹ in the upper section of Paglia River in November to 42 g day⁻¹ before the confluence with Tiber River in June. Along Tiber River, up to 15 ng L⁻¹ of the total Hg found at a site after Rome showed that Hg can be discharged to the sea. The Alviano reservoir along Tiber River acts as a temporary trap for Hg-rich particulate, while dam operations may promote Hg release (up to 223 g day⁻¹). The combination of hydrologic factors controlling Hg transport, the torrential regime in the upper catchment of Paglia River, the waterway steepness, together with Hg-contaminated legacy sediments in the Paglia River floodplain, make the Paglia–Tiber River system a long-lasting intermittent source of Hg to Tiber River and the Mediterranean Sea. Full article

An abandoned open green space in the urban setting of the Municipality of Pisa (Tuscany, Italy) has been designed for renewal to foster the development of recreational activities and improve the lives of the surrounding communities. However, the geochemical site characterization revealed Pb, Cu, Zn and Hg concentrations in the soil exceeding the thresholds imposed by Italian regulations for residential use. Pb, Cu and Zn contents likely reflect the effects of urban vehicle traffic, while Hg contamination represents the legacy of a past artisanal tannery that used Hg(II)-chloride in leather processing in the mid-1900s. Mercury is widely distributed in the area, with the highest concentration in the uppermost soil layer, and reaching about 170 mg/kg in the common dandelion rhizosphere. Chemical extractions and thermal desorption experiments have indicated that most Hg is in the elemental free and matrix-bound fraction, with a possible minor amount (less than 4 wt%) of HgS and negligible methylated forms (0.1 wt%). The data suggest that soil processes could reduce Hg²⁺ to volatile Hg⁰. Mercury in groundwater, hosted in a shallow aquitard in the area, was below 0.2 µg/L. However, the presence of chloride in groundwater might result in the formation of Hg stable aqueous complexes, increasing Hg release from solids. Future water quality monitoring is hence recommended. The risk assessment highlighted that mercury in soil carries a risk of non-cancerous effects, in particular for children, posing the basis for management planning. Full article

Considering the increasing emergence of new contaminants, such as nanomaterials, mixing with legacy contaminants, including metal(loid)s, it becomes imperative to understand the toxic profile resulting from these interactions. This work aimed at assessing and comparing the individual and combined hepatotoxic and neurotoxic potential of titanium dioxide nanoparticles (TiO₂NPs 0.75–75 mg/L), cerium oxide nanoparticles (CeO₂NPs 0.075–10 μg/L), arsenic (As 0.01–2.5 mg/L), and mercury (Hg 0.5–100 mg/L) on human hepatoma (HepG2) and neuroblastoma (SH-SY5Y) cells. Viability was assessed through WST-1 (24 h) and clonogenic (7 days) assays and it was affected in a dose-, time- and cell-dependent manner. Higher concentrations caused greater toxicity, while prolonged exposure caused inhibition of cell proliferation, even at low concentrations, for both cell lines. Cell cycle progression, explored by flow cytometry 24 h post-exposure, revealed that TiO₂NPs, As and Hg but not CeO₂NPs, changed the profiles of SH-SY5Y and HepG2 cells in a dose-dependent manner, and that the cell cycle was, overall, more affected by exposure to mixtures. Exposure to binary mixtures revealed either potentiation or antagonistic effects depending on the composition, cell type and time of exposure. These findings prove that joint toxicity of contaminants cannot be disregarded and must be further explored. Full article

Total mercury (THg) and methylmercury (MeHg) concentrations were analyzed in zooplankton (≥450 and ≥850 µm size fractions) collected seasonally over 6 years in Lake Maggiore (Northern Italy), characterized by a legacy mercury contamination. Analysis of δ ¹⁵N and δ¹³C stable isotopes was carried out to trace how taxa with different trophic levels and carbon sources contributed to mercury concentrations and trends. THg ranged between 44–213 µg kg⁻¹ d.w. and MeHg 15–93 µg kg⁻¹ d.w., representing 24–61% of THg. Values showed strong seasonal variations, with peaks in winter, due to the high biomass of predator taxa (Bythotrephes longimanus, Leptodora kindtii) and of Daphnia longispina-galeata gr. A positive correlation between THg and MeHg and δ¹⁵N signature was observed. D. longispina-galeata gr. prevailed in both size fractions, substantially contributing to THg and MeHg concentrations. Δ¹³C signature was strictly bound to lake thermal circulation dynamics. Mercury stock in the zooplankton compartment ranged between 19–140 ng THg m⁻² and 6–44 ng MeHg m⁻² for the ≥450 µm size fraction and between 2–66 ng THg m⁻² and 1–7 ng MeHg m⁻² for the ≥850 µm fraction, with the highest values in spring when zooplanktivorous fish actively prey in the pelagic zone. The results highlighted the crucial role of zooplankton as a repository of mercury, easily available to higher trophic levels. Full article

Mercury mining and its use in gold mine operations left a legacy of contamination in northern California. Contaminated sediments and water continue to affect local and downstream ecosystems. To assess the efficacy of biochar-amended soils on decreasing Hg transport, biochar was used to amend rock and sediment columns and mesocosms to decrease suspended sediment and associated mercury (Hg) in storm water runoff from Sierra Nevada hydraulic mines. Mercury-contaminated storm water runoff and hydraulic mine debris were collected from two hydraulic mine sites in the Yuba River, California watershed. Mercury concentrations and turbidity were analyzed from storm water samples and hydraulic mine debris in three simulated storm runoff experiments using decomposed granite columns, sediment columns, and sediment mesocosms amended at 0%, 2%, or 5% biochar by weight. Columns containing hydraulic mine debris and mixed with 5% biochar had a significant (p < 0.05) reduction in filter-passed mercury (FHg) in the outflow as compared to control columns. To simulate saturated hydraulic mine debris runoff, mesocosms were filled with mine sediment and saturated with deionized water to generate runoff. Five percent biochar in mesocosm trays decreased FHg significantly (p < 0.001), but, because of the angle of the tray, sediment also moved out of the trays. Biochar was effective at reducing FHg from hydraulic mine discharge. Biochar in laboratory columns with decomposed granite or mine sediments was more effective at removing Hg than mesocosms. Full article

Mercury (Hg) contamination in the Gulf of Trieste (northern Adriatic Sea) due to mining activity in Idrija (Slovenia) still represents an issue of environmental concern. The Isonzo/Soča River’s freshwater inputs have been identified as the main source of Hg into the Gulf, especially following periods of medium-high discharge. This research aims to evaluate the occurrence and distribution of dissolved (DHg) and particulate (PHg) Hg along the water column in the northernmost sector of the Gulf, a shallow and sheltered embayment suitable for the accumulation of fine sediments. Sediment and water samples were collected under unperturbed and perturbed environmental conditions induced by natural and anthropogenic factors. Mercury in the sediments (0.77–6.39 µg g⁻¹) and its relationship to grain size were found to be consistent with previous research focused on the entire Gulf, testifying to the common origin of the sediment. Results showed a notable variability of DHg (<LOD–149 ng L⁻¹) and PHg (0.39–12.5 ng L⁻¹) depending on the interaction between riverine and marine hydrological conditions. Mercury was found to be mainly partitioned in the suspended particles, especially following periods of high discharge, thus confirming the crucial role of the river inputs in regulating PHg distribution in the Gulf. Full article