Search Results (864)

This study aimed to investigate the effects of pterostilbene (PTE) on the intestinal barrier function, antioxidant capacity, lipid metabolism, and microbial and metabolite homeostasis of suckling piglets via its action on breast milk. Findings indicate that PTE supplementation enhanced the antioxidant status of mature milk and strengthened intestinal barrier function in piglets. Specifically, PTE enhanced intestinal antioxidant status and fatty acid β-oxidation in piglets by regulating the PI3K-AKT and SIRT1-Nrf2/Keap1 signaling pathways. 16S rDNA sequencing and Liquid Chromatography–Mass Spectroscopy (LC–MS) identified breast milk and gut microbiota and their metabolites, respectively. Results indicate that PTE significantly elevated levels of amino acid derivatives in colostrum (Glutathione Reducedform (GSH) and N-acetyl-L-glutamate (NAG)), whilst concurrently reducing levels of glycerophospholipid-related metabolites in both colostrum and mature milk (p < 0.05). Moreover, PTE supplementation markedly altered the composition of the colonic mucosal microbiota in piglets, with Faecalibacterium, Mucispirillum and Ruminococcus identified as key beneficial microbial markers of the colonic mucosa. Combined multi-omics revealed strong correlations in microbial community composition between mature milk and the colon, identifying glycerophospholipid metabolism as a key metabolic pathway that may be associated with the regulatory effects of PTE on milk and the piglet colon. In conclusion, the PTE supplement can improve the quality of breast milk and have a positive impact on the intestinal homeostasis of the offspring. Full article

Objective: The purpose of this study was to compare the influence of biological maturity status and training experience on motor performance in young athletes of different sport disciplines. Methods: Youth athletes (n = 84, 23 females) from five different sports (basketball, volleyball, track and field, wrestling, and badminton) participated in this study. Jump height was measured for the squat jump (SJ) and countermovement jump (CMJ). Peak torque during maximal voluntary contractions (MVCs) and torque steadiness at 20% MVC were assessed during plantar flexion (PF) and dorsiflexion (DF). Postural control was assessed with the one-leg test for both the right and left legs. K-means clustered analysis categorized participants into groups of low and high performers. Results: High performers had greater training experience than low performers for the SJ (p < 0.05), with no difference in maturity status (p > 0.05). Similarly, high performers had greater training experience (p < 0.05) than low performers for the CMJ, with no difference in maturity status (p > 0.05). High performers were more mature than low performers for MVC torque of DF (p < 0.001) and PF (p < 0.001), with no group differences in training experience (DF: p > 0.05; PF: p > 0.05). Maturity status for torque steadiness differed only for DF (p < 0.001), whereas there was no difference for PF (p > 0.05). There were no differences in either maturity status or training experience for one-leg-stance time (p > 0.05). Conclusions: These findings suggest that maturity status and training experience are linked to performance, although their relative roles differ across tasks. These findings reflect an interaction between biological maturity, training background and sports performance in youth athletes. Full article

Sea urchins are invertebrates that play a crucial role in marine ecosystems by controlling benthic algal communities and whose natural populations are being affected by different biotic and abiotic factors. Triggering physiological processes promotes the activation of certain metabolic pathways, so oxidative status markers could be a suitable tool to asses maturation stage in which natural populations are. Antioxidant status of three species of Mediterranean Sea urchins, A. lixula, P. lividus and S. granularis, was evaluated in gonadal and digestive tissue. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione s-transferase (GST), glucose 6-phosphate dehydrogenase (G6PDH), NAD(P)H: quinone oxidoreductase (NQO1) and lipid peroxidation were assayed. Significant differences were found among species, displaying in general higher antioxidant activity in A. lixula and S. granularis compared to P. lividus. A significant effect of sex was observed with females exhibiting a higher gonadosomatic index and higher levels of lipid peroxidation mainly in A. lixula. These results seem to be related to metabolic fluctuations associated with the gonadal maturation stage. Changes in digestive tissue were less evident, but some differences among species could be related to triggered digestive processes for replenishment of energy reserves in gonads. Oxidative status can be a useful complementary tool to evaluate gonadal condition in species of sea urchin from the same habitat. Integrative physiological and biochemical studies will contribute to the knowledge of invertebrate physiology. Full article

Lower-limb strength is a health and performance indicator in adolescents, although its assessment often fails to account for the influence of sex and biological development. This study aimed to analyze the associations between anthropometric parameters, maturational status, and physical activity levels with jumping performance in adolescents. A cross-sectional study was conducted with male and female adolescents (mean age: 13.60 ± 1.50 years). Anthropometric variables, maturational status, and physical activity levels were assessed. Performance was measured using the Countermovement Jump (CMJ) and Standing Broad Jump (SBJ). In males, jumping performance was significantly associated with height (p = 0.002), lower-limb length (p < 0.001), and muscle mass (p < 0.001). However, fat mass emerged as a substantial factor, exhibiting a large effect size on performance (p < 0.001). Maturational status in males showed significant differences, with late maturers performing lower than on-time and early maturers (p < 0.023). In females, structural anthropometry and maturation showed limited-to-no significant associations with performance, except for a negative association with fat mass (p < 0.035) and a positive association between muscle mass and CMJ (p < 0.020). Active adolescents of both sexes performed significantly better than inactive ones in both CMJ and SBJ (p < 0.011). In conclusion, jumping performance in adolescents is characterized by marked sexual dimorphism. In males, greater height, lower-limb length, lower fat mass, and early maturation are positively associated with superior performance. Conversely, in females, these factors exhibit limited influence on jump outcomes. Full article

Purpose: The primary aim of the present study was to examine the extent to which participation in organized youth basketball training contributes to physical activity across intensity zones during training sessions in relation to biological maturation status. Methods: Participants were classified into three maturity groups based on predicted age at peak height velocity (PHV): −2.5 to −1.5, −1.5 to −0.5, and ≥−0.5 to 0.83 years from PHV. Data from two training sessions per participant were averaged to obtain representative individual values. One-way analyses of variance (ANOVAs) were used to examine differences in anthropometric, physical performance, and field performance variables between PHV groups. Physical activity patterns were analyzed using two-way mixed-design ANOVAs with PHV stage as the between-subject factor and intensity zone (MET- and HRR-based) as the within-subject factor. Results: Across all maturity groups, approximately 10–17% of total training time was spent in light-intensity activity, while the majority of time was accumulated in moderate-to-vigorous intensity zones (approximately 35–50%, depending on the classification method). Significant maturity-related differences were observed in anthropometric variables and physical performance measures, with more mature players demonstrating superior sprint performance, jumping ability, and grip strength. Field performance indicators also differed between PHV groups, with more mature athletes exhibiting higher external and internal training loads. In contrast, no significant interactions or main effects of PHV stage were observed for physical activity intensity distribution. Conclusions: Organized basketball training contributes substantially to moderate-to-vigorous physical activity accumulated during training sessions. However, these findings reflect training-specific activity and should not be interpreted as representing total daily physical activity. No differences in activity intensity distribution were observed between maturation groups, although this finding should be interpreted with caution, given methodological limitations. These results highlight the need to consider biological maturation when designing youth training programs. Full article

Background/Objectives: The growth status of children and adolescents with discrepancies of maxilla/mandible during the growing period should be closely monitored to determine the appropriate time to begin growth modification in orthodontic treatment. Skeletal growth assessment using the cervical vertebral maturation (CVM) method is widely used and accepted; however, monitoring requires additional doses of radiation. Thus, this study aimed to evaluate tooth development from routine panoramic radiographs to represent the growth status rather than using the CVM method. Methods: Three hundred and sixty pairs of lateral cephalometric and panoramic radiographs (180 males and 180 females) aged 7–15 years were included. Teeth 31–37 of each panoramic radiograph were identified as A to H according to the Demirjian method, and the stages of skeletal growth were indicated from lateral cephalometric radiographs using the CVM method. The relationship between tooth development and CVM was analyzed using Spearman’s rank correlation coefficients. Results: The correlation coefficients ranged from 0.487 to 0.768 for male subjects and from 0.503 to 0.759 for female subjects. Tooth 33 was found to have the highest correlation in males (r = 0.768) and tooth 37 was revealed to have the highest correlation in females (r = 0.759) (p < 0.01). Conclusions: Teeth 33–37 showed correlation coefficients close to 0.7 or above, which indicated a moderate-to-high correlation between tooth development and CVM. Thus, the pattern of tooth development from teeth 33–37 may serve as a supplementary indicator of skeletal maturation timing which was similar in both males and females, and may serve as a supplementary indicator of skeletal maturation timing. Full article

Background and Objectives: Childhood obesity is associated with alterations in body composition that may impair muscular strength and functional capacity. While higher body mass is often accompanied by greater absolute strength, the independent effect of adiposity on muscle strength after accounting for lean mass remains insufficiently understood. This study aimed to evaluate the associations between adiposity and muscle strength in children and adolescents, while accounting for growth and maturation, and to examine differences according to weight status. Materials and Methods: This retrospective cross-sectional study included 84 children and adolescents aged 5–18 years. Anthropometric measurements were used to calculate body mass index (BMI), waist-to-hip ratio, and waist-to-height ratio, with weight status classified according to CDC BMI-for-age percentiles. Body composition was assessed using bioelectrical impedance analysis (Tanita). Pubertal stage was evaluated using Tanner classification. Muscle strength was assessed using dominant handgrip strength. Associations between adiposity-related parameters and muscle strength were analyzed using correlation and multivariable linear regression models adjusted for age, sex, pubertal stage, physical activity, and body composition. Results: Body mass index was positively correlated with absolute handgrip strength (r = 0.561, p < 0.001). Body fat percentage was negatively associated with relative handgrip strength (r = −0.381, p < 0.001). In multivariable regression analyses, body fat percentage remained an independent negative predictor of handgrip strength (β = −0.203, p = 0.0046), whereas fat-free mass and fat-free mass index were positive predictors in respective models (p < 0.001). Conclusions: Increased adiposity is associated with reduced muscle strength in children and adolescents when strength is evaluated relative to body size or adjusted for lean mass. These findings support the concept of impaired muscle performance in pediatric populations with excess adiposity and highlight the importance of integrating body composition and functional assessments in clinical evaluation. Full article

Over two years, a randomized complete block field trial tested deficit irrigation [I: 70% ETc; NI] and ammonium nitrate [N₀, N₆₀, N₁₂₀; 0, 60, 120 kg N ha⁻¹] application in two northern Greece winegrape vineyards of cv. ‘Xinomavro’ (XM) and cv. ‘Cabernet Sauvignon’ (CS). Leaf-blade δ¹⁵N was measured at berry set, bunch closure, veraison, and technological maturity; berry-juice (must) δ¹⁵N at technological maturity and dormant cane δ¹⁵N in winter were also determined. In the first year, δ¹⁵N was additionally measured in petioles, unripe berries, trunks, and roots, along with arbuscular mycorrhizal fungal (AMF) colonization of fine roots. Fertilization increased δ¹⁵N in leaf blades and canes, whereas berry-juice δ¹⁵N responded weakly and inconsistently. Irrigation marginally lowered cane δ¹⁵N; cane δ¹⁵N varied between years, and berry-juice δ¹⁵N showed the highest variability across treatments. At berry set, intravine discrimination was evident: young berries and leaf blades were enriched, while fine roots and woody tissues were depleted. Root δ¹⁵N responses differed between cultivars and depended on AMF colonization in XM. Leaf and cane δ¹⁵N were positively related to vine N status, yield, and pruning weight but negatively to agronomic N-use efficiency indices. These findings indicate that δ¹⁵N serves as an integrative proxy of N cycling processes and fertilizer-use efficiency in vineyards, with potential implications for the assessment and optimization of sustainable vineyard management practices in the context of climate change. Full article

Background: The first 1000 days of life, from conception to 2 years of age, represent a critical window during which nutrition can exert long-lasting effects on neurodevelopment, immune maturation, and susceptibility to prematurity-related morbidity. Docosahexaenoic acid (DHA) is a key structural n-3 long-chain polyunsaturated fatty acid of the brain and retina, characterized by rapid fetal accretion during the third trimester. Methods: We conducted a narrative review of studies published from March 2015 up to December 2025, including randomized controlled trials, follow-up studies, and systematic reviews/meta-analyses about DHA supplementation during pregnancy, lactation, infancy and early childhood, and its role on development. Results: Across the first 1000 days, DHA supplementation improves biochemical DHA status, particularly in populations with low baseline levels (moderate to high level of evidence), while clinical outcomes remain heterogeneous. During pregnancy, some benefits in specific cognitive and behavioral domains have been demonstrated, whereas effects on global cognition and long-term behavior are frequently null (moderate evidence). Visual outcomes appear favorable, with improvements in visual acuity (moderate evidence). In preterm infants, enteral DHA—often combined with arachidonic acid (ARA)—is feasible and well tolerated. DHA may reduce inflammatory markers and necrotizing enterocolitis risk when in equilibrium with ARA (low to moderate evidence), while no evidence supports the link between DHA and reduced risk of bronchopulmonary dysplasia and retinopathy of prematurity (moderate evidence). Neurodevelopmental outcomes are mixed: neuroimaging studies suggest enhanced white matter maturation with DHA + ARA, whereas most trials show no clear benefit regarding standardized developmental scores (moderate evidence). Conclusions: DHA is biologically essential during the first 1000 days, but its clinical impact depends on timing, dose, baseline status, and prematurity-related context. The balance between DHA and ARA, rather than DHA supplementation alone, emerges as a key determinant of clinical efficacy, supporting a shift toward precision-based nutritional strategies in early life. Full article

Drip fertigation (DF) is increasingly adopted to improve winter wheat productivity, yet its interactions with planting density (PD) and the underlying source–sink mechanisms remain insufficiently quantified. Here, we evaluated winter wheat performance under two water–nitrogen (N) regimes—conventional management (CM) and DF—across a wide PD gradient (100–800 seeds m⁻²) during two growing seasons. Grain yield, yield components, population traits, dry matter production, source–sink indices, canopy N status, N uptake and N-use efficiencies were assessed. Across seasons, DF increased grain yield by 15.4–20.8% relative to CM. Yield exhibited a quadratic response to PD under both regimes; however, DF shifted the optimal PD upward (456–487 seeds m⁻²) compared with CM (377–378 seeds m⁻²) and sustained near-maximum yields over a broader PD range. DF improved population productivity by increasing productive stem percentage and grains per ear, resulting in greater grain number per m² (sink size). DF also strengthened source capacity during grain filling: post-anthesis dry matter production increased by 15.5–17.6% and strongly associated with yield (r² ≥ 0.819). Source–sink analysis suggested that DF was associated with more density treatments showing simultaneously high grain number and high post-anthesis dry matter accumulation, a pattern consistent with a broader high-yield density range. Enhanced N acquisition, especially after anthesis, may have contributed to this response. DF increased N nutrition index at anthesis and markedly increased post-anthesis N uptake by 47.7–49.5%, thereby raising total N uptake at maturity and grain N accumulation. DF improved fertilizer-N recovery efficiency and agronomic efficiency by 33.9–42.3% and 26.7–30.9%, respectively. Collectively, DF improved N uptake and source–sink coordination, enabling high yield and reduced yield penalties when planting density deviated from the optimum. Full article

Objective: This randomized controlled trial aimed to investigate how volume-matched unilateral and bilateral jump training affects physical performance in prepubertal and pubertal male youth soccer players and to examine whether maturational status influences these training adaptations. Methods: Sixty-five male soccer players (age 10.5 ± 2.9 years; height 136.7 ± 17.8 cm; body mass 32.8 ± 8.6 kg; maturity offset −1.6 ± 1.0 years) completed an 8-week training program (two sessions/week). Participants were randomly assigned to a bilateral jump group (n = 22), unilateral jump group (n = 22), or control group (n = 21). Performance was evaluated in a single testing session, which included horizontal jump tests (bilateral standing long jump and single-leg hop distance), linear sprint tests over 10 m (acceleration) and 30 m (maximal sprint performance) using timed trials, and change-of-direction (COD) ability assessed via a standardized timed COD test. Results: Significant main effects of time, maturation, and time × group interactions were observed for all outcomes (p ≤ 0.013). A maturation × group interaction was found for bilateral jump performance (p = 0.045), a group effect for 10 m sprint time (p = 0.015), and a time × maturation × group interaction for COD performance (p < 0.001). Both training groups had improved jump performance (jump distance) and 10 m sprint time across maturity levels, while no changes were observed in the control group. For 30 m sprint time, improvements were observed in both training groups in prepubertal players, whereas only the unilateral group showed improvements in pubertal players. COD performance (completion time) improved in the unilateral group at both maturity levels and in the bilateral group at the pubertal level. Conclusions: Structured jump training enhances horizontal jump distance, sprint performance, and COD ability in youth soccer players. Adaptations appear to be influenced by training modality and maturation, although these effects may vary depending on the specific performance task. Full article

Background: Artificial intelligence-based computer-aided detection (AI-CAD) systems are increasingly being used in endovascular practice to support time-sensitive detection, triage and prioritization tasks in imaging and procedural workflows. Despite rapid technological advancements and expanding regulatory clearances, the translation to lasting clinical benefit varies. Objective: This narrative review synthesizes AI-CAD applications in endovascular interventions and proposes an evaluation-oriented framework to support responsible clinical translation; this framework emphasizes detection-specific metrics, external validation, bias-aware assessment, and workflow integration. Methods: A structured narrative review was conducted using targeted searches in PubMed, Google Scholar, and IEEE Xplore (2020–2026); this review was supported by an examination of US FDA device databases and citation tracking. Evidence was assessed using a pragmatic hierarchical classification framework based on regulatory status and validation rigor. Results: AI-CAD applications were mapped across four main endovascular domains: neurovascular interventions (e.g., large vessel occlusion triage), coronary interventions (CCTA-based stenosis detection and intravascular imaging support), aortic interventions/EVAR (endoleak detection and sac monitoring), and peripheral interventions (lesion detection and angiographic decision support). Across the domains, performance reporting was heterogeneous and often relied on retrospective, single-center assessments. Key barriers to clinical readiness included acquisition variability and dataset shift due to artifacts, limited multicenter validation, annotation variability, and human–AI workflow factors. Evaluation priorities included whether to assess at the lesion level or case level, false positive burden and calibration, external validation under real-world heterogeneity, and clinical impact measures such as treatment timing and procedural decision-making. Conclusions: AI-CAD systems hold significant potential for improving endovascular care; however, clinical readiness depends on rigorous, endovascular feature-specific assessment and transparent reporting, beyond retrospective accuracy. The proposed evidence level framework and assessment checklist provide practical tools for distinguishing mature technologies from research prototypes and guiding future validation, implementation, and post-market monitoring. Full article

Background: This study examined the effects of an individualized weighted vest sprint training program on sprint performance and countermovement jump (CMJ) outcomes in post-peak height velocity (PHV) male youth soccer players while accounting for maturation status. Methods: Fifty players (mean age 17.76 ± 0.95 years) were randomly assigned to a weighted vest sprint group (WVG; n = 25) or a traditional unloaded sprint group (TS; n = 25). Sprint performance (5, 10, 20, and 30 m) and CMJ-derived variables (jump height, peak power output, reactive strength index modified (RSI-modified), and eccentric rate of force development) were assessed before and after an 11-week intervention performed twice weekly, with the WVG completing sprint drills while wearing a weighted vest (~11% body mass). Results: Weighted vest sprint training produced greater improvements in 10 m sprint performance and RSI-modified (d = 1.37 and 1.55, respectively). However, after Benjamini–Hochberg adjustment for multiple comparisons, the effects were no longer statistically significant and should therefore be interpreted with caution. Maturity offset did not meaningfully moderate training-induced adaptations. Conclusions: These findings suggest that weighted vest sprint training may provide potential benefits for mid-acceleration performance and reactive strength in post-PHV youth soccer players, although the magnitude of these effects remains uncertain. Full article

Evapotranspiration and crop coefficients are key variables for designing efficient irrigation strategies in tree crops, yet standard tabulated coefficients derived for mature, fully covering orchards often fail to represent the water use of young, high-density hazelnut systems. In recent years, updated crop coefficients for temperate fruit trees, including hazelnut, and transpiration-based models have been proposed, while several studies have successfully linked Vegetation Indices and thermal metrics to single and basal crop coefficients in vineyards, orchards and field crops. However, no information is available on the use of UAV-derived spectral and thermal indices to estimate crop coefficients in high-density hazelnut orchards. This study compares crop coefficients obtained from traditional approaches (the FAO56 single crop coefficient, a transpiration-based coefficient, and ground cover reduction factors) with coefficients estimated from UAV-derived Normalized Difference Water Index (NDWI) and Crop Water Stress Index (CWSI) in a subsurface-drip-irrigated hazelnut orchard (cv. Tonda Francescana^®) with two planting densities (625 and 1250 trees ha⁻¹) in central Italy. Multispectral and thermal UAV surveys carried out between 2021 and 2024 were used to derive canopy geometrical traits, ground cover, NDWI, and CWSI, while a local weather station provided reference evapotranspiration. Empirical relationships were calibrated between crop coefficients and ground cover, NDWI, and CWSI, and mid-season coefficients were applied to estimate daily crop evapotranspiration, which was then compared with the irrigation volumes supplied during the 2024 season. The standard FAO56 crop coefficient (Kc = 0.9) overestimated evapotranspiration, especially at the lower planting density, whereas ground cover-based reduction factors recalibrated for hazelnut and the transpiration-based coefficient provided estimates more consistent with the applied irrigation. UAV-based NDWI- and CWSI-derived crop coefficients produced mid-season values close to those obtained with the transpiration-based method for both planting densities, confirming that spectral and thermal information can effectively capture the combined effects of canopy development and water status. These results indicate that combining traditional methods with UAV-derived indices offers a flexible framework to refine crop coefficients in high-density hazelnut orchards and support more accurate and spatially explicit irrigation scheduling. Full article

Brassicaceae plants are generally considered non-mycorrhizal; however, recent studies have challenged this non-host status, suggesting occasional colonization during reproductive stages or by overlooked fungi such as Mucoromycotina Fine Root Endophytes (MFRE). To re-evaluate the non-host status of Brassicaceae, we cultivated five Brassicaceae species, including rapid life cycle Brassica rapa (Fast plants) using field soil containing both Glomeromycotina Arbuscular Mycorrhizal Fungi (G-AMF) and MFRE. To ensure inoculum potential, a co-planting system with lettuce (Lactuca sativa) as a nurse plant was employed. While lettuce roots were rapidly colonized by both G-AMF and MFRE, no mycorrhizal colonization was observed in any Brassicaceae roots throughout their entire life cycle, from vegetative growth to flowering and seed maturation in Fast plants. Furthermore, co-planting with Brassicaceae did not significantly affect the mycorrhizal colonization or shoot phosphorus concentrations of the neighboring lettuce. These results demonstrate that Brassicaceae plants maintain a robust non-host status against both G-AMF and MFRE. Moreover, they function as “neutral non-hosts” that do not disrupt the symbiotic networks of neighboring plants. This characteristic reinforces the value of Brassicaceae in sustainable crop rotation systems. Full article