2.1. Description of the Experimental Design
The research was carried out in an experimental orchard managed by the Department of Agricultural, Food, and Environmental Sciences of the University of Perugia, located in central Italy (4760920N, 288120E EPSG:7792-RDN2008/UTM Zone 33N), at 161 m a.s.l. (orthometric height) (
Figure 1), during the growing seasons 2021–2024.
The orchard features a split-plot design with three planting densities as main plots:
- -
625 trees ha−1 (4 × 4 m spacing);
- -
1250 trees ha−1 (4 × 2 m spacing);
- -
2500 trees ha−1 (4 × 1 m spacing, experimental treatment).
The 2500 trees ha
−1 density was excluded from analyses for two scientifically justified reasons: (1) At tree age 7 (planted 2017), this density causes excessive inter-tree shading (>80% row occlusion, measured via hemispherical photography), reducing net canopy photosynthesis despite high PAR interception (>95%) [
22]. (2) It exceeds current commercial recommendations (≤1500 trees ha
−1) due to observed yield quality penalties in preliminary trials [
23].
All densities include both cultivars. divided into two contiguous but separate planting blocks by variety: Rows 1–3: Tonda di Giffoni (later TG); Rows 4–6: Tonda Francescana
® (later TF) (
Figure 1). In each planting block, there are three planting densities from north to south, starting from the densest (2500 trees ha
−1) to the least dense (625 trees ha
−1). All trees are grafted on
Corylus colurna L. non-suckering rootstock and trained as single trunks. Analyses focused exclusively on TF due to: (1) its higher commercial relevance and yield [
9]; and the (2) complete UAV coverage availability across all plots. No significant cultivar × density interactions were detected (ANOVA, F = 1.23,
p = 0.31;
Table S1), justifying cultivar-specific reporting.
The orchard was equipped with a subsurface drip irrigation (SDI) system configured with two lines of Neptune PC AS dripline (pressure-compensating and anti-siphon) (50 cm emitter spacing, 2.4 L/hour flow rate), buried at a depth of 30 cm. Moreover, n. 2 soil moisture sensors (SM100) were installed at depths of 30 cm and 50 cm to monitor soil water content during the growing season.
Meteorological data were monitored by a Spectrum (Thayer Court, Aurora) WatchDog 2000 Series weather station located within the experimental site.
For the growing season 2024, irrigation was applied from 2 July 2024 to 19 August 2024, with a total water volume of 210 m3. Irrigation was managed with a constant dose (or time) each day, except when precipitation occurred.
2.2. UAV Surveys
Multispectral and thermal UAV surveys were conducted on 18 July 2023 and 8 July 2024 at solar noon (±30 min) under clear-sky conditions (
Table S2).
Multispectral surveys were performed using a DJI (Shenzhen, China) Phantom 4 Multispectral P4M (P4M [
24],
Figure 2a), flying at 10 m altitude with 75% forward and side overlap and a speed of 1.5–2 m s
−1, resulting in a ground sample distance (GSD) of approximately 1.5 cm pixel
−1. The green (560 ± 16 nm) and near-infrared (840 ± 26 nm) bands were used to compute the Normalized Difference Water Index (NDWI). A spectral sunlight sensor enabled real-time irradiance correction during image acquisition.
Thermal surveys were conducted using a DJI (Shenzhen, China) Mavic 3T (M3T [
25];
Figure 2b), flying at 12 m altitude with 75% frontal and lateral overlap and a speed of 1.5–2 m s
−1. The thermal sensor provides a spatial resolution of 640 × 512 pixels and 8-bit radiometric data. Thermal image pre-processing was performed using the DJI (Shenzhen, China) Thermal Decoder (SDK-based), which converts raw digital numbers (DN) into temperature-calibrated TIFF images [
26].
Both UAV platforms were operated using RTK positioning with a D-RTK 2 mobile station (
Figure 2c), supporting GPS, BeiDou, GLONASS, and Galileo constellations to achieve centimeter-level georeferencing accuracy. Flight missions were planned using DJI (Shenzhen, China) GS Pro (iPad).
The resulting multispectral orthophotos were used to derive canopy traits and NDWI, while thermal orthophotos were used to compute the Crop Water Stress Index (CWSI) (
Section 2.3).
Figure 2d summarizes the overall methodological workflow, including UAV data acquisition, data processing, and the dual estimation of crop coefficients (traditional vs. UAV-based), followed by a consistency check against the irrigation volumes recorded in 2024.
In this study, images acquired by the multispectral sensor were used to extract tree geometrical features, such as canopy mean diameter and height, and to generate NDWI map; thermal sensor data were employed to produce thermal maps, estimate canopy temperature and generate CWSI maps.
2.4. Estimation of Crop Coefficients Using Traditional Methods
In this study, the estimation of crop coefficients was done using literature values or applying methods valuable for hazelnut or other fruit crops, as follows:
The literature values of the single and basal crop coefficients for temperate climate fruit trees, as assessed by López-Urrea et al. [
12];
The method proposed by Vinci et al. [
27] under the hypothesis that the evaporative component of the crop evapotranspiration can be negligible and the crop coefficient can be assimilated to the transpiration coefficient (k
c,Tr);
The procedure described by Fereres et al. [
28], where the crop coefficient depends on canopy ground cover.
As assessed by López-Urrea et al. [
12], studies referring to hazelnut (
Corylus avellana L.) [
27,
29,
30,
31,
32,
33] reported a range of k
c/k
cb values based on the fraction of ground cover fc, the height h, and the training system.
As assessed by [
27], the transpiration coefficient (k
c,Tr) tested on hazelnut for both densities, 625 tree/ha and 1250 tree/ha, could be evaluated as follows:
where Q
d is the radiation intercepted by the tree (fraction); F
1 depends on tree density, i.e., F
1 = 0.66 for tree densities >250 trees/ha; F
2 is the monthly tabulated coefficient = 1.25 for June and July; e = 2.718; K
ext is the radiation extinction coefficient; d
p is the tree density (trees/ha); DAF is the leaf area density; V
u is the canopy volume per unit ground surface (m
3/m
2); V
0 is the canopy volume (m
3/tree); D is the canopy’s average diameter (m); and H is the canopy height (m).
The approach by [
27] assumes that the soil evaporation component of ET
c is negligible in these micro-irrigated orchards, so that k
c can be assimilated to a transpiration coefficient k
c,Tr. This assumption is reasonable for subsurface drip, where the wetted soil surface is minimal, but may not hold under different irrigation layouts or soil conditions.
The procedure described by [
28] suggests defining a reduction coefficient k
r,t of the crop coefficient k
c to account for a sparsely planted orchard (such as young orchards). Thus, k
r,t is an empirical coefficient of an orchard with incomplete cover relative to a mature orchard. The original k
r,t relationship was developed for young almond orchards; here it is recalibrated using UAV-derived ground cover data to obtain hazelnut-specific coefficients. As suggested by [
34], the k
r,t coefficient is related to the horizontal projection of tree shade (ground cover). The relationship between percent ground cover (G%) and k
r,t for almond trees was [
28]:
with a = −0.00012 and b = 0.0226.
Almond (
Prunus dulcis) [
35] and hazelnut (
Corylus avellana) [
9,
36] share deciduous canopy architecture, central-leader training systems, comparable leaf area index (LAI 2.5–4.0), and subsurface drip irrigation, which justifies the initial application of almond coefficients as a baseline prior to hazelnut-specific recalibration.
Two-step approach:
Test almond coefficients [Equation (7)];
Recalibrate for hazelnut using UAV-derived ground cover (G, 2021–2023) via Equation (17) to obtain hazelnut-specific coefficients [Equations (18) and (19)].
Canopy diameter (D) and height (H) extracted from multispectral point clouds (
Section 2.3.1) enabled ground cover (G) estimation for recalibration.
2.5. Estimation of Crop Coefficients Using Vegetation Indices (VIs) from UAV Surveys
Choudhury et al. [
37] explored the theoretical basis for using Vegetation Indices (VIs) to replace k
c-FAO. Using wheat as a model crop, they showed the following:
where Tr
c is a plant transpiration coefficient (by analogy with k
c) and VI* is a vegetation index stretched between 0 (representing bare soil) and fully transpiring, unstressed vegetation, using the formula:
VI
max and VI
min are determined from satellite image statistics for individual scenes [
38,
39].
The exponent η is determined by the relationship between transpiration and the VI used in Equation (8).
Traditional ground-based approaches for kc estimation (e.g., sap flow sensors, lysimeters, and manual ground cover measurements) face significant limitations in high-density hazelnut orchards:
Spatial sampling bias: Point measurements miss intra-orchard variability driven by differential canopy development, soil heterogeneity, and microclimate gradients [
4].
Labor and cost: Continuous monitoring with sap flow or lysimeters is impractical at commercial scales.
Temporal resolution: Ground methods cannot provide frequent, wall-to-wall coverage during rapid canopy growth phases.
UAV-based NDWI overcomes these limitations by providing high-resolution (1.5 cm/pixel), spatially continuous maps of canopy water status and ground cover fraction. The empirical VI*-kc relationships (Equations (8) and (9)) [
37] have been validated across diverse crops, with power–law exponents (η ≈ 0.3) consistent with our findings, confirming their physical basis linking spectral reflectance to transpiring leaf area.
As assessed above, in this paper the Normalized Difference Water Index (NDWI) and the Crop Water Stress Index (CWSI) were selected as indices relevant to crop water status and used for the estimation of the crop coefficient.
The NDWI map of the entire study area (
Figure 6a) was derived from the multispectral orthophoto using Equation (10). The NDWI was subsequently restricted to canopy areas by masking the raster with canopy polygons obtained from the ground projection of the canopy point clouds (
Figure 6b). The CWSI was then calculated according to Equation (12) from the thermal map previously masked to canopy areas (
Figure 6c). All raster-based calculations were performed in QGIS software (v. 3.34.10).
The Normalized Difference Water Index (NDWI) formulation used in this paper is [
38]:
where Green and NIR are the green and near-infrared bands respectively.
In a GIS environment, the orthophoto derived from corrected thermic images, was used to obtain the thermal map with the formula:
where T
meas = the temperature registered in the pixel; G
v = the digital number reported in the thermal orthophoto; T
max = the maximum temperature; T
min = the minimum temperature; and N
grey = the total number of values that the pixel can assume, in this case 8-bit, corresponding to 2
8 = 256 values.
Thermal remote sensing complements spectral indices by monitoring canopy temperature (T
c) and deriving the Crop Water Stress Index (CWSI), originally formulated from the relationship between canopy–air temperature difference and vapor pressure deficit. In its empirical and theoretical versions, CWSI has been widely used as an indicator of crop water status and for irrigation management in different crops [
39,
40]. Simplified CWSI formulations based on wet and dry reference surfaces have been proposed to avoid the explicit derivation of non-water-stressed baselines under variable environmental conditions [
41,
42,
43].
In this study, canopy temperatures obtained from the thermal camera were used to compute a simplified CWSI, obtained by considering the highest canopy temperature recorded in the image as the dry pixel and the lowest canopy temperature as the wet pixel [
20]. Specifically, the following formula was applied [
44]:
where T
c = canopy temperature (°C); T
a = air temperature (°C); T
cl = temperature of a non-stressed canopy (°C); and T
cu = temperature of a stressed canopy (°C). In practice, to reduce the influence of residual mixed pixels, T
cu and T
cl were derived from the 90th and 10th percentiles of the canopy temperature distribution within each image, respectively.
As assessed by [
42] on almonds, canopy temperature and CWSI exhibit substantial intra-crown variability driven by structural heterogeneity and local water status [
40,
41]. Similar analyses using high-resolution thermal imagery have shown that CWSI variability within the canopy is closely related to stomatal conductance and transpiration patterns, and that robust estimates benefit from focusing on the coldest, purest vegetation pixels [
43]. At the individual tree level, differences in liquid-phase resistance to flow between the trunk base and the various parts of the top of the canopy determine variations in water supply that can influence stomatal conductance and T
c. Variability in current and previous radiation exposure can also generate T
c differences between various parts of the tree crown. Furthermore, changes in leaf angle distribution, leaf area density, and canopy architecture may affect T
c. To reduce these effects, for each tree and planting density, the median Tc value, as well as the 10th percentile and 90th percentile, were considered to compute CWSI for each tree.
2.6. Validation of Crop Coefficients Estimated by Traditional vs. Vegetation Indices
The daily crop evapotranspiration ET
c,i, under standard conditions, can be estimated using the k
c-ET
0 approach [
45], i.e., as the product between the reference crop evapotranspiration ET
0 and the crop coefficient k
c:
The daily series of reference evapotranspiration, ET
0 (mm/day), for the 2024 growing season, were calculated using the FAO Penman–Monteith equation [
10]:
where R
n is the net radiation at the crop surface (MJ m
2 day
−1), G is the soil heat flux density (MJ m
−2 day
−1), T is the mean daily air temperature (°C), u
2 is the wind speed at 2 m height (m/s), e
s is the saturation vapor pressure (kPa), e
a is the actual vapor pressure (kPa), ∆ is the slope vapor pressure curve (kPa °C
−1) and γ is the constant psychrometric (kPa °C
−1).
Meteorological data collected by the meteorological station were used to evaluate the ET0.
The crop coefficients for the mid-season derived using the traditional methods (kc, kc,Tr, kr,t, ) and the Vegetation Indices (kc-NDWI and kc-CWSI) were applied to Equation (13) to evaluate the best performance, considering that the term ET0 depends only on the meteorological characteristics of the 2024growing season.