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Keywords = marine environmental element field

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38 pages, 6505 KiB  
Review
Trends in Oil Spill Modeling: A Review of the Literature
by Rodrigo N. Vasconcelos, André T. Cunha Lima, Carlos A. D. Lentini, José Garcia V. Miranda, Luís F. F. de Mendonça, Diego P. Costa, Soltan G. Duverger and Elaine C. B. Cambui
Water 2025, 17(15), 2300; https://doi.org/10.3390/w17152300 - 2 Aug 2025
Viewed by 232
Abstract
Oil spill simulation models are essential for predicting the oil spill behavior and movement in marine environments. In this study, we comprehensively reviewed a large and diverse body of peer-reviewed literature obtained from Scopus and Web of Science. Our initial analysis phase focused [...] Read more.
Oil spill simulation models are essential for predicting the oil spill behavior and movement in marine environments. In this study, we comprehensively reviewed a large and diverse body of peer-reviewed literature obtained from Scopus and Web of Science. Our initial analysis phase focused on examining trends in scientific publications, utilizing the complete dataset derived after systematic screening and database integration. In the second phase, we applied elements of a systematic review to identify and evaluate the most influential contributions in the scientific field of oil spill simulations. Our analysis revealed a steady and accelerating growth of research activity over the past five decades, with a particularly notable expansion in the last two. The field has also experienced a marked increase in collaborative practices, including a rise in international co-authorship and multi-authored contributions, reflecting a more global and interdisciplinary research landscape. We cataloged the key modeling frameworks that have shaped the field from established systems such as OSCAR, OIL-MAP/SIMAP, and GNOME to emerging hybrid and Lagrangian approaches. Hydrodynamic models were consistently central, often integrated with biogeochemical, wave, atmospheric, and oil-spill-specific modules. Environmental variables such as wind, ocean currents, and temperature were frequently used to drive model behavior. Geographically, research has concentrated on ecologically and economically sensitive coastal and marine regions. We conclude that future progress will rely on the real-time integration of high-resolution environmental data streams, the development of machine-learning-based surrogate models to accelerate computations, and the incorporation of advanced biodegradation and weathering mechanisms supported by experimental data. These advancements are expected to enhance the accuracy, responsiveness, and operational value of oil spill modeling tools, supporting environmental monitoring and emergency response. Full article
(This article belongs to the Special Issue Advanced Remote Sensing for Coastal System Monitoring and Management)
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18 pages, 9518 KiB  
Article
CFD-Based Parameter Calibration and Design of Subwater In Situ Cultivation Chambers Toward Well-Mixing Status but No Sediment Resuspension
by Liwen Zhang, Min Luo, Shanggui Gong, Zhiyang Han, Weihan Liu and Binbin Pan
J. Mar. Sci. Eng. 2025, 13(7), 1290; https://doi.org/10.3390/jmse13071290 - 30 Jun 2025
Viewed by 232
Abstract
The elemental exchange fluxes at the sediment–water interface play a crucial role in Earth’s climate regulation, environmental change, and ecosystem dynamics. Accurate in situ measurements of these fluxes depend heavily on the performance of marine incubation devices, particularly their ability to achieve full [...] Read more.
The elemental exchange fluxes at the sediment–water interface play a crucial role in Earth’s climate regulation, environmental change, and ecosystem dynamics. Accurate in situ measurements of these fluxes depend heavily on the performance of marine incubation devices, particularly their ability to achieve full mixing without causing sediment resuspension. This study presents a novel parameter calibration method for a marine in situ incubation device using a combination of computational fluid dynamics (CFD) simulations and laboratory experiments. The influence of the stirring paddle’s rotational speed on flow field distribution, complete mixing time, and sediment resus-pension was systematically analyzed. The CFD simulation results were validated against existing device data and actual experimental measurements. The deviation in complete mixing time between simulation and experiment was within −9.23% to 9.25% for 20 cm of sediment and −9.4% to 9.1% for 15 cm. The resuspension tests determined that optimal mixing without sediment disturbance occurs at rotational speeds of 25 r/min and 35 r/min for the two sediment depths, respectively. Further analysis showed that the stirring paddle effectively creates a uniform flow field within the chamber. This CFD-based calibration method provides a reliable approach to parameter tuning for various in situ devices by adjusting boundary conditions, offering a scientific foundation for device design and deployment, and introducing a new framework for future calibration efforts. Full article
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13 pages, 4099 KiB  
Article
Study of Electrochemical Behavior and a Material Removal Mechanism During Electrolytic Plasma Polishing of 316L Stainless Steel
by Gangqiang Ji, Longfei Ma, Sunan Zhang, Juan Zhang and Liyun Wu
Materials 2025, 18(6), 1307; https://doi.org/10.3390/ma18061307 - 16 Mar 2025
Cited by 1 | Viewed by 670
Abstract
Electrolytic plasma polishing technology is widely used in medical devices, aerospace, nuclear industry, marine engineering, and other equipment manufacturing fields, owing to its advantages of shape adaptability, high efficiency, good precision, environmental protection, and non-contact polishing. However, the lack of in-depth research on [...] Read more.
Electrolytic plasma polishing technology is widely used in medical devices, aerospace, nuclear industry, marine engineering, and other equipment manufacturing fields, owing to its advantages of shape adaptability, high efficiency, good precision, environmental protection, and non-contact polishing. However, the lack of in-depth research on the material removal mechanism of the electrolytic plasma polishing process severely restricts the regulation of the process parameters and polishing effect, leading to optimization and improvement by experimental methods. Firstly, the formation mechanism of passivation film was revealed based on an analysis of the surface morphology and chemical composition of stainless steel. Subsequently, the dissolution mechanism of the passivation film was proposed by analyzing the change in the valence state of the main metal elements on the surface. In addition, the surface enclosure leveling mechanism of electrolytic plasma polishing (EPP) for stainless steel was proposed based on a material removal mechanism model combined with experimental test methods. The results show that EPP significantly reduces the surface roughness of stainless steel, with Ra being reduced from 0.445 µm to 0.070 µm. Metal elements on the anode surface undergo electrochemical oxidation reactions with reactive substances generated by the gas layer discharge, resulting in the formation of passivation layers of metal oxides and hydroxides. The passivation layer complexes with solvent molecules in the energetic plasma state of the gas layer with SO42− ions, forming complexes that enter the electrolyte. The dynamic balance between the formation and dissolution of the passivation film is the key to achieving a flat surface. This study provides theoretical guidance and technical support for the EPP of stainless steel. Full article
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20 pages, 7908 KiB  
Article
Numerical Simulation of Typhoon Waves in an Offshore Wind Farm Area of the South China Sea
by Baofeng Zhang, Xu Li, Lizhong Wang and Yangyang Gao
J. Mar. Sci. Eng. 2025, 13(3), 451; https://doi.org/10.3390/jmse13030451 - 26 Feb 2025
Cited by 1 | Viewed by 910
Abstract
Environmental load data are an essential input for the analysis of offshore wind structures in typhoon-prone marine environments. However, numerical simulations of typhoon waves lack a systematic examination of the specific influence of typhoon trajectories on the spatial evolution of wave fields. In [...] Read more.
Environmental load data are an essential input for the analysis of offshore wind structures in typhoon-prone marine environments. However, numerical simulations of typhoon waves lack a systematic examination of the specific influence of typhoon trajectories on the spatial evolution of wave fields. In particular, the intricate mechanisms governing wave propagation within wind farm areas remain poorly understood. This present study, drawing upon a real-world case in an offshore wind farm area in the South China Sea, employs the Finite Volume Coastal Ocean Model–Surface Wave Module (FVCOM–SWAVE) wave–current coupling model to assess the joint wind–wave distribution characteristics during 35 typhoon events. The findings reveal that typhoon wave fields exhibit a notable rightward bias. As waves approach the coast, the significant wave height decreases progressively due to wave breaking, friction, refraction, and nonlinear interactions. During the passage of typhoons Prapiroon, Hato, and Mangkhut, the significant wave height distribution in the wind farm area closely correlated with the wind speed distribution. By constructing a joint distribution function of sea wind and wave elements, the joint distribution characteristics of wind speed and significant wave height for different return periods can be obtained, providing important oceanic environmental inputs for the design analysis of offshore wind structures. Full article
(This article belongs to the Special Issue Advances in Offshore Wind—2nd Edition)
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18 pages, 27264 KiB  
Article
Research on Visualization Methods for Marine Environmental Element Fields in Twin Spaces
by Lianwei Li, Shiyu Wu, Cunjin Xue, Yingying Ma and Qunce Qin
J. Mar. Sci. Eng. 2025, 13(3), 449; https://doi.org/10.3390/jmse13030449 - 26 Feb 2025
Cited by 1 | Viewed by 546
Abstract
Visualization of marine environmental field elements is one of the core technologies in marine science research. Particularly in the context of “digital twin of the ocean” (DTO) construction and application, accurately reproducing the dynamic evolution of marine environmental elements remains a critical challenge. [...] Read more.
Visualization of marine environmental field elements is one of the core technologies in marine science research. Particularly in the context of “digital twin of the ocean” (DTO) construction and application, accurately reproducing the dynamic evolution of marine environmental elements remains a critical challenge. Existing visualization methods are primarily limited to static displays and fail to achieve deep integration and dynamic expression of marine environmental elements and sea conditions. To address this, this paper proposes a new visualization method for marine environmental element fields in a twin-space framework. This method first constructs dynamic wave twin objects based on wave data, then develops a marine environmental element field model based on environmental element data, and finally generates a coupled marine environmental element twin object through integration. This approach facilitates the dynamic and collaborative visualization of multiple marine elements. The collaborative visualization results of wave information, ocean surface temperature, and salinity data indicate that this method effectively enhances the dynamic expression capability and information fusion level of the ocean environment feature field. It provides new ideas and approaches for the development of ocean environment visualization technology in twin spaces, with promising application potential. Full article
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16 pages, 4792 KiB  
Article
Wind Turbine Aerodynamics Simulation Using the Spectral/hp Element Framework Nektar++
by Hamidreza Abedi and Claes Eskilsson
Wind 2025, 5(1), 6; https://doi.org/10.3390/wind5010006 - 18 Feb 2025
Cited by 1 | Viewed by 709
Abstract
Wind power plays an increasingly vital role in sustainable energy development. However, accurately simulating wind turbine aerodynamics, particularly in offshore wind farms, remains challenging due to complex environmental factors such as the marine atmospheric boundary layer. This study investigates the integration and assessment [...] Read more.
Wind power plays an increasingly vital role in sustainable energy development. However, accurately simulating wind turbine aerodynamics, particularly in offshore wind farms, remains challenging due to complex environmental factors such as the marine atmospheric boundary layer. This study investigates the integration and assessment of the Actuator Line Model (ALM) within the high-order spectral/hp element framework, Nektar++, for wind turbine aerodynamic simulations. The primary objective is to evaluate the implementation and effectiveness of the ALM by analyzing aerodynamic loads, wake behavior, and computational demands. A three-bladed NREL-5MW turbine is modeled using the ALM in Nektar++, with results compared against established computational fluid dynamics (CFD) tools, including SOWFA and AMR-Wind. The findings demonstrate that Nektar++ effectively captures velocity and vorticity fields in the turbine wake while providing aerodynamic load predictions that closely align with finite-volume CFD models. Furthermore, the spectral/hp element framework exhibits favorable scalability and computational efficiency, indicating that Nektar++ is a promising tool for high-fidelity wind turbine and wind farm aerodynamic research. Full article
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26 pages, 7383 KiB  
Review
A Review of Research Progress on Cables and Towed Objects of the Ocean Engineering Towing System
by Kefu Qi, Jianing Zhang, Lei Zhang, Jinlong Zhang and Ruijun Gan
J. Mar. Sci. Eng. 2025, 13(2), 257; https://doi.org/10.3390/jmse13020257 - 30 Jan 2025
Cited by 2 | Viewed by 1410
Abstract
Towing operations are widely applied in various fields such as maritime accident rescue, assisting large vessels entering and exiting ports, and transporting large ocean platforms. Tugboats and the towed objects form a complex multi-body system connected by flexible cables, and during operations, they [...] Read more.
Towing operations are widely applied in various fields such as maritime accident rescue, assisting large vessels entering and exiting ports, and transporting large ocean platforms. Tugboats and the towed objects form a complex multi-body system connected by flexible cables, and during operations, they are subjected to the effects of complex marine environmental loads. Current research focuses on using numerical simulations and model tests in water tanks to study the motion response of towed objects and cables under the action of environmental loads. There is a lack of research that combines the mechanical response and structural strength with the load conditions of towing operations. Taking cables as an example, most studies focus on the mechanical properties of cables without considering the impact of towing conditions. After reviewing the literature, this paper summarizes the shortcomings of the existing research and points out several potential research directions in the field of towing: the mechanical response of cables during the initial stage of towing, experiments on towing by multiple tugboats, research on composite fiber cables using experimental and finite element simulation methods, and structural optimization of components related to towing operations. Full article
(This article belongs to the Special Issue Advanced Research in Flexible Riser and Pipelines)
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22 pages, 1898 KiB  
Review
Cyanoremediation of Polluted Seawater in the Arabian Gulf: Risks and Benefits to Human Health
by R. F. Al-Thani and B. T. Yasseen
Processes 2024, 12(12), 2733; https://doi.org/10.3390/pr12122733 - 2 Dec 2024
Cited by 1 | Viewed by 1423
Abstract
Cyanobacteria play a crucial role in marine ecosystems as primary producers of food and oxygen for various organisms while helping remove waste and toxic substances from the environment. They are essential to the carbon cycle and help regulate the climate. These marine autotrophs [...] Read more.
Cyanobacteria play a crucial role in marine ecosystems as primary producers of food and oxygen for various organisms while helping remove waste and toxic substances from the environment. They are essential to the carbon cycle and help regulate the climate. These marine autotrophs also aid in the absorption of essential elements and support diverse life forms. They help degrade organic compounds, including petroleum hydrocarbons as well as heavy metals. Fluctuations in cyanobacteria populations can indicate ecosystem health, influencing both human well-being and wildlife. Their significance also extends to potential technological advancements, thus providing valuable resources for fields such as pharmacology, medicine, health care, biofuels, cosmetics, and bioremediation. However, some species produce toxins that pose risks to human health and marine organisms. Consequently, cyanobacteria are a major focus of research aimed at preserving and improving marine ecosystems—especially given the environmental damage caused by past and potential future conflicts. This review highlights their roles in cyanoremediation and other industrial and biotechnological applications with a particular focus on the Arabian Gulf region. Full article
(This article belongs to the Section Sustainable Processes)
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25 pages, 9279 KiB  
Article
Implementation of a Far-Field Water Quality Model for the Simulation of Trace Elements in an Eastern Mediterranean Coastal Embayment Receiving High Anthropogenic Pressure
by Aikaterini Anna Mazioti, Vassilis Kolovoyiannis, Evangelia Krasakopoulou, Elina Tragou, Vassilis Zervakis, Georgia Assimakopoulou, Alexandros Athiniotis, Vasiliki Paraskevopoulou, Alexandra Pavlidou and Christina Zeri
J. Mar. Sci. Eng. 2024, 12(5), 797; https://doi.org/10.3390/jmse12050797 - 10 May 2024
Cited by 3 | Viewed by 2345
Abstract
Water quality modeling is a key element for the support of environmental protection and policymaking. The aim of this work is to describe the application of a far-field water quality model for the simulation of marine pollution occurring from heavy metals (cadmium, lead, [...] Read more.
Water quality modeling is a key element for the support of environmental protection and policymaking. The aim of this work is to describe the application of a far-field water quality model for the simulation of marine pollution occurring from heavy metals (cadmium, lead, nickel, copper, and zinc). The highly stressed marine area of the Saronikos Gulf (Aegean Sea, Eastern Mediterranean) was chosen for investigation. Major pollution sources were identified, loads were estimated, and the model was parameterized to reproduce the local seawater conditions. The distribution of the pollutants between the dissolved and particulate phases was examined. The performance of the model set-up was evaluated using field concentration measurements. The described implementation succeeded in reproducing the observed levels of pollution and therefore can be used as a baseline configuration to examine the cumulative impact of future pollution sources; for example, accidental pollution events. Full article
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53 pages, 6636 KiB  
Review
Seaweeds and Corals from the Brazilian Coast: Review on Biotechnological Potential and Environmental Aspects
by Gustavo Souza dos Santos, Thais Luz de Souza, Thaiz Rodrigues Teixeira, João Pedro Cezário Brandão, Keila Almeida Santana, Luan Henrique Santos Barreto, Samantha de Souza Cunha, Daniele Cristina Muniz Batista dos Santos, Conor R. Caffrey, Natan Silva Pereira and Aníbal de Freitas Santos Júnior
Molecules 2023, 28(11), 4285; https://doi.org/10.3390/molecules28114285 - 23 May 2023
Cited by 8 | Viewed by 4659
Abstract
Brazil has a megadiversity that includes marine species that are distributed along 800 km of shoreline. This biodiversity status holds promising biotechnological potential. Marine organisms are important sources of novel chemical species, with applications in the pharmaceutical, cosmetic, chemical, and nutraceutical fields. However, [...] Read more.
Brazil has a megadiversity that includes marine species that are distributed along 800 km of shoreline. This biodiversity status holds promising biotechnological potential. Marine organisms are important sources of novel chemical species, with applications in the pharmaceutical, cosmetic, chemical, and nutraceutical fields. However, ecological pressures derived from anthropogenic actions, including the bioaccumulation of potentially toxic elements and microplastics, impact promising species. This review describes the current status of the biotechnological and environmental aspects of seaweeds and corals from the Brazilian coast, including publications from the last 5 years (from January 2018 to December 2022). The search was conducted in the main public databases (PubChem, PubMed, Science Direct, and Google Scholar) and in the Espacenet database (European Patent Office—EPO) and the Brazilian National Property Institute (INPI). Bioprospecting studies were reported for seventy-one seaweed species and fifteen corals, but few targeted the isolation of compounds. The antioxidant potential was the most investigated biological activity. Despite being potential sources of macro- and microelements, there is a literature gap regarding the presence of potentially toxic elements and other emergent contaminants, such as microplastics, in seaweeds and corals from the Brazilian coast. Full article
(This article belongs to the Special Issue Bioactives and Functional Compounds from Marine Plants)
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19 pages, 10295 KiB  
Article
Improved Design of LNG Marine Loading Arm Docking Method Based on TRIZ Theory
by Jie Mei, Wuwei Feng and Zirong Liang
Appl. Sci. 2023, 13(7), 4525; https://doi.org/10.3390/app13074525 - 3 Apr 2023
Cited by 7 | Viewed by 4380
Abstract
The LNG marine loading arm is a critical component for transferring LNG from vessels to onshore receiving stations. However, currently, the operation of LNG marine loading arms is still faced with issues such as a slow docking speed and significant environmental impacts from [...] Read more.
The LNG marine loading arm is a critical component for transferring LNG from vessels to onshore receiving stations. However, currently, the operation of LNG marine loading arms is still faced with issues such as a slow docking speed and significant environmental impacts from waves and wind. In this paper, we propose a set of feasible improvement schemes for the traditional LNG marine loading arm based on the theory of inventive problem solving (TRIZ). We utilized tools such as functional models, causal chain analysis, contradiction analysis, and Su-Field model analysis to develop these schemes. Our proposed improvements include using machine vision and automatic control to replace manual work, conducting finite element analysis and topology optimization of the LNG marine loading arm to improve its structure, and innovating the design of the end structure of the LNG discharge arm. Full article
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20 pages, 2361 KiB  
Review
Recovery of Bioactive Compounds from Marine Organisms: Focus on the Future Perspectives for Pharmacological, Biomedical and Regenerative Medicine Applications of Marine Collagen
by Salvatrice Rigogliuso, Simona Campora, Monica Notarbartolo and Giulio Ghersi
Molecules 2023, 28(3), 1152; https://doi.org/10.3390/molecules28031152 - 24 Jan 2023
Cited by 31 | Viewed by 7473
Abstract
Marine environments cover more than 70% of the Earth’s surface and are among the richest and most complex ecosystems. In terms of biodiversity, the ocean represents an important source, still not widely exploited, of bioactive products derived from species of bacteria, plants, and [...] Read more.
Marine environments cover more than 70% of the Earth’s surface and are among the richest and most complex ecosystems. In terms of biodiversity, the ocean represents an important source, still not widely exploited, of bioactive products derived from species of bacteria, plants, and animals. However, global warming, in combination with multiple anthropogenic practices, represents a serious environmental problem that has led to an increase in gelatinous zooplankton, a phenomenon referred to as jellyfish bloom. In recent years, the idea of “sustainable development” has emerged as one of the essential elements of green-economy initiatives; therefore, the marine environment has been re-evaluated and considered an important biological resource. Several bioactive compounds of marine origin are being studied, and among these, marine collagen represents one of the most attractive bio-resources, given its use in various disciplines, such as clinical applications, cosmetics, the food sector, and many other industrial applications. This review aims to provide a current overview of marine collagen applications in the pharmacological and biomedical fields, regenerative medicine, and cell therapy. Full article
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20 pages, 10769 KiB  
Article
Sedimentological Analysis of Regional Differentiation and Sediment Provenance in the Lu’erhuan River Sea Area of Qinzhou Bay, Guangxi Province
by Ping Li, Jun Du, Zhiwei Zhang and Guoqiang Xu
J. Mar. Sci. Eng. 2022, 10(11), 1732; https://doi.org/10.3390/jmse10111732 - 11 Nov 2022
Cited by 1 | Viewed by 1982
Abstract
Globally, coastal regions are vital areas of human activity and, as such, are centers of population growth and urban and economic development. Long-term human development has had a major impact on the ecological environment of coastal zones. Therefore, exploring the distribution and provenance [...] Read more.
Globally, coastal regions are vital areas of human activity and, as such, are centers of population growth and urban and economic development. Long-term human development has had a major impact on the ecological environment of coastal zones. Therefore, exploring the distribution and provenance of marine sediment types in coastal areas heavily influenced by human activities can provide scientific evidence and references for the current and future ecological management of these sensitive environments. For this reason, we conducted an analysis of the sediment grain size, endmembers, and organic matter content and geochemical elements in the Lu’erhuan River-Malan Island-Sandun Island area in the eastern part of Qinzhou Bay, a region heavily influenced by human activities. The sediment grain size clearly differs throughout the study site and the material provenances and hydrodynamic conditions also vary, likely due to the local environmental conditions and the significant impact that human activities have had on the area. The finest-grained sediment is imported from either inland or coastal areas via rivers and weak tidal currents, the next finest component comes from coastal areas through weak tidal currents, and the moderately coarse component mainly originates from nearby beaches. The two coarsest-grained sediment components are influenced by the combination of human activities, tidal currents and waves and enter the water via erosion. The organic matter provenance resembles that of the sediment components, exhibiting varied characteristics. Due to the combination of natural and human activities in the bay, the organic matter in the upper reaches of the Lu’erhuan River originates from the river and coastal paddy fields, with obvious terrigenous characteristics; the organic matter in northern Malan Island mainly comes from external sources related to oyster farming, while the organic matter in eastern Sandun Island is mainly produced endogenously by marine plankton. Al, Ti, Fe, Mg, K, Ga and other elements indicate that terrestrial sediments are significantly disturbed by human activities. However, Mn reflects the marine distribution of terrestrial sediments from the Lu’erhuan River to Jishuimen. Ca and Sr, which are indicators of marine sediments, are distributed in the eastern offshore area of Sandun Island, which is connected to open waters. Due to the influence of human activities, As and Cd are highly enriched in the study area, while Cu is less affected by human activities. Full article
(This article belongs to the Section Geological Oceanography)
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23 pages, 7942 KiB  
Article
Geochemical Analysis of Cretaceous Shales from the Hazara Basin, Pakistan: Provenance Signatures and Paleo-Weathering Conditions
by Abdul Ghaffar Fazal, Muhammad Umar, Faisal Shah, Muhammad Armaghan Faisal Miraj, Hammad Tariq Janjuhah, George Kontakiotis and Abdul Khaliq Jan
J. Mar. Sci. Eng. 2022, 10(6), 800; https://doi.org/10.3390/jmse10060800 - 10 Jun 2022
Cited by 15 | Viewed by 4381 | Correction
Abstract
The geochemical investigation of shales from the Early to Middle Cretaceous Chichali Formation in the Hazara Basin was conducted to determine the origin, tectonic setting and evolution, paleo-weathering conditions, and paleo-oceanographic reconstruction. The research included a comprehensive field survey, sample collection, and analysis [...] Read more.
The geochemical investigation of shales from the Early to Middle Cretaceous Chichali Formation in the Hazara Basin was conducted to determine the origin, tectonic setting and evolution, paleo-weathering conditions, and paleo-oceanographic reconstruction. The research included a comprehensive field survey, sample collection, and analysis of a variety of main, trace, and rare-earth elements using an X-ray fluorescence spectrometer (XRF). Bivariate plots and ternary diagrams were used to determine the provenance, tectonic setting, and paleo-weathering conditions that existed during the development of the Chichali Formation in the Hazara Basin. The values of Ba/Sc, Ba/Co, Th/Sc, Cr/Th, Cr/Zr, Th/Co, Th/Cr, Sc/Th, bivariate plots of Al2O3 vs. TiO2, TiO2 vs. Zr, TiO2 vs. Ni, Df1–Df2, Zr vs. Nb, and La/Sc vs. Th/Co, and ternary diagram of K2O–Fe2O3–Al2O3 were used to illustrate the passive continental margin setting of Chichali Formation shales. The detailed chemical analysis also provides an understanding of the marine geochemical cycle, which reflects the origin of these sediments. The average K2O/Al2O3 value is less than 0.4, indicating that the shale contains clay minerals. The Chichali Formation’s Chemical Index of Alteration (mean = 71) and Index of Compositional Variation (mean = 1.12) values show a modest degree of chemical weathering in the source locations. From an environmental standpoint, the Chichali Formation is richer in toxic elements such as Ba, Zn, Ni, Cr, and Cu, which may be damaging to agricultural soils and drinking water when present in excess. These metals are incorporated into the formation during the weathering process. Full article
(This article belongs to the Special Issue Recent Advances in Geological Oceanography)
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19 pages, 3302 KiB  
Article
A Novel SELEX Based on Immobilizing Libraries Enables Screening of Saxitoxin Aptamers for BLI Aptasensor Applications
by Rong Zhou, Yun Gao, Chengfang Yang, Xiaojuan Zhang, Bo Hu, Luming Zhao, Han Guo, Mingjuan Sun, Lianghua Wang and Binghua Jiao
Toxins 2022, 14(3), 228; https://doi.org/10.3390/toxins14030228 - 21 Mar 2022
Cited by 13 | Viewed by 4323
Abstract
Saxitoxin (STX) is one of the potent marine biotoxins that has high rate of lethality. However, there are no effective treatments at present, and the existing detection methods need to be further explored because of ethical problems or technical limitations. In this work, [...] Read more.
Saxitoxin (STX) is one of the potent marine biotoxins that has high rate of lethality. However, there are no effective treatments at present, and the existing detection methods need to be further explored because of ethical problems or technical limitations. In this work, oligonucleotide aptamers toward STX were screened based on immobilizing libraries on Immobilized Metal-Chelate (IMC), such as Ni-NTA Sepharose, and the IMC-SELEX was conducted by the G-quadruplex library and the random library, respectively. Aptamer 45e (from the G-quadruplex library) and aptamer 75a were obtained after optimization, and aptamer 45e turned out to have a higher affinity toward STX. Furthermore, it was found that the hydrogen bonding and the van der Waals forces (VDW) played major roles in the high efficiency and specificity between STX and 45e by means of molecular docking and dynamics simulation. Based on this, aptamer 45e-1 with the Kd value of 19 nM was obtained by further optimization, which was then used to construct a simple, label-free and real-time optical BLI aptasensor for the detection of STX. This aptasensor showed good reproducibility and stability. In summary, with the advantages of screening aptamers of high efficiency and specificity toward the targets, the proposed IMC-SELEX provides a promising screening strategy for discovering aptamers, which could be used as the potential molecular recognition elements in the fields of biomedicine, food safety and environmental monitoring. Full article
(This article belongs to the Special Issue Marine Biotoxins: Predicting and Cumulative Risk Assessment)
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