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30 pages, 7530 KB  
Review
Probing the Sources of Ultra-High-Energy Cosmic Rays—Constraints from Cosmic-Ray Measurements
by Teresa Bister
Universe 2025, 11(10), 331; https://doi.org/10.3390/universe11100331 - 3 Oct 2025
Abstract
Ultra-high-energy cosmic rays (UHECRs) are the most energetic particles known—and yet their origin is still an open question. However, with the precision and accumulated statistics of the Pierre Auger Observatory and the Telescope Array, in combination with advancements in theory and modeling—e.g., of [...] Read more.
Ultra-high-energy cosmic rays (UHECRs) are the most energetic particles known—and yet their origin is still an open question. However, with the precision and accumulated statistics of the Pierre Auger Observatory and the Telescope Array, in combination with advancements in theory and modeling—e.g., of the Galactic magnetic field—it is now possible to set solid constraints on the sources of UHECRs. The spectrum and composition measurements above the ankle can be well described by a population of extragalactic, homogeneously distributed sources emitting mostly intermediate-mass nuclei. Additionally, using the observed anisotropy in the arrival directions, namely the large-scale dipole >8 EeV, as well as smaller-scale warm spots at higher energies, even more powerful constraints on the density and distribution of sources can be placed. Yet, open questions remain—like the striking similarity of the sources that is necessary to describe the rather pure mass composition above the ankle, or the origin of the highest energy events whose tracked back directions point toward voids. The current findings and possible interpretation of UHECR data will be presented in this review. Full article
13 pages, 2617 KB  
Article
Kilowatt-Level EUV Regenerative Amplifier Free-Electron Laser Enabled by Transverse Gradient Undulator in a Storage Ring
by Changchao He, Nanshun Huang, Tao Liu, Changliang Li, Bo Liu and Haixiao Deng
Photonics 2025, 12(10), 983; https://doi.org/10.3390/photonics12100983 - 2 Oct 2025
Abstract
High-average-power extreme ultraviolet (EUV) sources are essential for large-scale nanoscale chip manufacturing, yet commercially available laser-produced plasma sources face challenges in scaling to the kilowatt level. We propose a novel scheme that combines the high repetition rate of a diffraction-limited storage ring with [...] Read more.
High-average-power extreme ultraviolet (EUV) sources are essential for large-scale nanoscale chip manufacturing, yet commercially available laser-produced plasma sources face challenges in scaling to the kilowatt level. We propose a novel scheme that combines the high repetition rate of a diffraction-limited storage ring with a regenerative amplifier free-electron laser (RAFEL) employing a transverse gradient undulator (TGU). By introducing dispersion in the storage ring, electrons of different energies are directed into corresponding magnetic field strengths of the TGU, thereby satisfying the resonance condition under a large energy spread and increasing the FEL gain. Simulations show that at equilibrium, the average EUV power exceeds 1 kW, with an output pulse energy reaching ∼2.86 μJ, while the energy spread stabilizes at ∼0.45%. These results demonstrate the feasibility of ring-based RAFEL with TGU as a promising route toward kilowatt-level EUV sources. Full article
(This article belongs to the Special Issue Next-Generation X-Ray Optical Technologies and Applications)
15 pages, 1392 KB  
Article
Optimal Source Selection for Distributed Bearing Fault Classification Using Wavelet Transform and Machine Learning Algorithms
by Ramin Rajabioun and Özkan Atan
Appl. Sci. 2025, 15(19), 10631; https://doi.org/10.3390/app151910631 - 1 Oct 2025
Abstract
Early and accurate detection of distributed bearing faults is essential to prevent equipment failures and reduce downtime in industrial environments. This study explores the optimal selection of input signal sources for high-accuracy distributed fault classification, employing wavelet transform and machine learning algorithms. The [...] Read more.
Early and accurate detection of distributed bearing faults is essential to prevent equipment failures and reduce downtime in industrial environments. This study explores the optimal selection of input signal sources for high-accuracy distributed fault classification, employing wavelet transform and machine learning algorithms. The primary contribution of this work is to demonstrate that robust distributed bearing fault diagnosis can be achieved through optimal sensor fusion and wavelet-based feature engineering, without the need for deep learning or high-dimensional inputs. This approach provides interpretable, computationally efficient, and generalizable fault classification, setting it apart from most existing studies that rely on larger models or more extensive data. All experiments were conducted in a controlled laboratory environment across multiple loads and speeds. A comprehensive dataset, including three-axis vibration, stray magnetic flux, and two-phase current signals, was used to diagnose six distinct bearing fault conditions. The wavelet transform is applied to extract frequency-domain features, capturing intricate fault signatures. To identify the most effective input signal combinations, we systematically evaluated Random Forest, XGBoost, and Support Vector Machine (SVM) models. The analysis reveals that specific signal pairs significantly enhance classification accuracy. Notably, combining vibration signals with stray magnetic flux consistently achieved the highest performance across models, with Random Forest reaching perfect test accuracy (100%) and SVM showing robust results. These findings underscore the importance of optimal source selection and wavelet-transformed features for improving machine learning model performance in bearing fault classification tasks. While the results are promising, validation in real-world industrial settings is needed to fully assess the method’s practical reliability and impact on predictive maintenance systems. Full article
(This article belongs to the Section Electrical, Electronics and Communications Engineering)
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16 pages, 7116 KB  
Article
Magnetotelluric Monitoring of Earthquake Precursors
by Alexander K. Saraev, Vadim Surkov, Vjacheslav Pilipenko, Arseny A. Shlykov, Nikita Bobrov, Mikhail Dembelov, Denis Zinkin and Sudha Agrahari
GeoHazards 2025, 6(4), 61; https://doi.org/10.3390/geohazards6040061 - 1 Oct 2025
Abstract
Approaches to magnetotelluric monitoring of variations in apparent resistivity and electromagnetic emission that may serve as earthquake precursors are considered. Monitoring of apparent resistivity is advised in the range 7–300 Hz, where natural electromagnetic fields exhibit stable behavior, while at lower frequencies the [...] Read more.
Approaches to magnetotelluric monitoring of variations in apparent resistivity and electromagnetic emission that may serve as earthquake precursors are considered. Monitoring of apparent resistivity is advised in the range 7–300 Hz, where natural electromagnetic fields exhibit stable behavior, while at lower frequencies the behavior of the electrotelluric and magnetic fields should be analyzed. We present results of studies aimed at identifying active faults and searching for stress–strain sensitive zones for installing measurement equipment based on the registration of tidal variations in apparent resistivity. The features of apparent resistivity anomalies preceding earthquakes in China based on direct current measurements are discussed. Based on the analysis of natural electromagnetic field monitoring in the ULF and ELF ranges in China, the anomalies recorded prior to several recent earthquakes are considered. Before the Yangbi earthquake (2017) and the series of Yangbi (2021) and Ninglang (2022) earthquakes, variations in apparent resistivity were observed that have a pulsed behavior and probably are manifestations of electromagnetic emission. Possible sources of these anomalies are active faults located near the monitoring stations. Full article
(This article belongs to the Special Issue Active Faulting and Seismicity—2nd Edition)
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10 pages, 532 KB  
Article
3D Non-Uniform Fast Fourier Transform Program Optimization
by Kai Nie, Haoran Li, Lin Han, Yapeng Li and Jinlong Xu
Appl. Sci. 2025, 15(19), 10563; https://doi.org/10.3390/app151910563 - 30 Sep 2025
Abstract
MRI (magnetic resonance imaging) technology aims to map the internal structure image of organisms. It is an important application scenario of Non-Uniform Fast Fourier Transform (NUFFT), which can help doctors quickly locate the lesion site of patients. However, in practical application, it has [...] Read more.
MRI (magnetic resonance imaging) technology aims to map the internal structure image of organisms. It is an important application scenario of Non-Uniform Fast Fourier Transform (NUFFT), which can help doctors quickly locate the lesion site of patients. However, in practical application, it has disadvantages such as large computation and difficulty in parallel. Under the architecture of multi-core shared memory, using block pretreatment, color block scheduling NUFFT convolution interpolation offers a parallel solution, and then using a static linked list solves the problem of large memory requirements after the parallel solution on the basis of multithreading to cycle through more source code versions. Then, manual vectorization, such as processing, using short vector components, further accelerates the process. Through a series of optimizations, the final Random, Radial, and Spiral dataset obtained an acceleration effect of 273.8×, 291.8× and 251.7×, respectively. Full article
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20 pages, 2506 KB  
Article
Design of an RRAM-Based Joint Model for Embedded Cellular Smartphone Self-Charging Device
by Abhinav Vishwakarma, Anubhav Vishwakarma, Matej Komelj, Santosh Kumar Vishvakarma and Michael Hübner
Micromachines 2025, 16(10), 1101; https://doi.org/10.3390/mi16101101 - 28 Sep 2025
Abstract
With the development of embedded electronic devices, energy consumption has become a significant design issue in modern systems-on-a-chip. Conventional SRAMs cannot maintain data after powering turned off, limiting their use in applications such as battery-powered smartphone devices that require non-volatility and no leakage [...] Read more.
With the development of embedded electronic devices, energy consumption has become a significant design issue in modern systems-on-a-chip. Conventional SRAMs cannot maintain data after powering turned off, limiting their use in applications such as battery-powered smartphone devices that require non-volatility and no leakage current. RRAM devices are recently used extensively in applications such as self-charging wireless sensor networks and storage elements, owing to their intrinsic non-volatility and multi-bit capabilities, making them a potential candidate for mitigating the von Neumann bottleneck. We propose a new RRAM-based hybrid memristor model incorporated with a permanent magnet. The proposed design (1T2R) was simulated in Cadence Virtuoso with a 1.5 V power supply, and the finite-element approach was adopted to simulate magnetization. This model can retain the data after the power is off and provides fast power on/off transitions. It is possible to charge a smartphone battery without an external power source by utilizing a portable charger that uses magnetic induction to convert mechanical energy into electrical energy. In an embedded smartphone self-charging device this addresses eco-friendly concerns and lowers environmental effects. It would lead to the development of magnetic field-assisted embedded portable electronic devices and open the door to new types of energy harvesting for RRAM devices. Our proposed design and simulation results reveal that, under usual conditions, the magnet-based device provide a high voltage to charge a smartphone battery. Full article
(This article belongs to the Special Issue Self-Tuning and Self-Powered Energy Harvesting Devices)
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29 pages, 5340 KB  
Article
Counteracting the Aging Process of the Aircraft’s DC Power Grid in the Context of Shaping the Characteristics of the Power Source
by Tomasz Tokarski, Sławomir Michalak and Tomasz Gajewski
Energies 2025, 18(19), 5132; https://doi.org/10.3390/en18195132 - 26 Sep 2025
Abstract
This article presents a proprietary method for measuring and adjusting the position of DC generator brushes relative to the neutral zone. This method enables shaping the characteristics of the power sources of the aircraft’s DC power grids, which has a beneficial effect on [...] Read more.
This article presents a proprietary method for measuring and adjusting the position of DC generator brushes relative to the neutral zone. This method enables shaping the characteristics of the power sources of the aircraft’s DC power grids, which has a beneficial effect on counteracting the aging process. Using generators with varying operating times (flight hours), selected research results on the effect of the brush position angle α relative to the magnetically neutral zone on operating conditions are presented. In addition, examples of abnormal operation of the DC electrical network in transient states and their possible negative effects on the operation of the aircraft (A/C) power network are presented. Finally, research results are presented demonstrating the practical application of power source characteristic shaping to effectively counteract the aging process of aircraft DC power networks. Full article
(This article belongs to the Special Issue Advances in Electrical Power System Quality)
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29 pages, 1662 KB  
Review
Adsorbent Materials Based on Modified Chitosan for Purification of Aqueous Media from Pharmaceutical Residues, Primarily Antibiotics
by Balzhima Shagdarova, Yulia Zhuikova and Alla Il’ina
Polymers 2025, 17(19), 2601; https://doi.org/10.3390/polym17192601 - 26 Sep 2025
Abstract
This literature review highlights the latest advances in the use of adsorption materials based on modified chitosan for the purification of aqueous solutions from pharmaceutical residues. Some countries are actively working to detect pharmaceuticals and their metabolites in water samples from natural sources [...] Read more.
This literature review highlights the latest advances in the use of adsorption materials based on modified chitosan for the purification of aqueous solutions from pharmaceutical residues. Some countries are actively working to detect pharmaceuticals and their metabolites in water samples from natural sources and municipal wastewater, as well as to study their impact on the environment. In this article, adsorbents based on chitosan, a natural, low toxic and biodegradable polymer, are considered as a promising solution to this problem. Due to some disadvantages of pure chitosan (low mechanical strength, small specific surface area), its practical application is limited. One of the ways to overcome them is to create modified materials, such as grafted copolymers, as well as chitosan derivatives and its composites, including those with magnetic nanoparticles and carbon materials. Modification of chitosan makes it possible to achieve an increase in mechanical strength, specific surface area and porosity. The high efficiency of hybrid adsorbents is emphasised, demonstrating high adsorption capacity, reuse ability and selectivity for a wide range of pharmaceutical preparations, including antibiotics. Thus, despite a number of limitations, chitosan-based materials are a promising solution for deep wastewater treatment. Full article
(This article belongs to the Section Polymer Applications)
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15 pages, 2559 KB  
Article
Quasi-Static and Dynamic Measurement Capabilities Provided by an Electromagnetic Field-Based Sensory Glove
by Giovanni Saggio, Luca Pietrosanti, I-Jung Lee and Bor-Shing Lin
Biosensors 2025, 15(10), 640; https://doi.org/10.3390/bios15100640 - 25 Sep 2025
Abstract
The sensory glove (also known as data or instrumented glove) plays a key role in measuring and tracking hand dexterity. It has been adopted in a variety of different domains, including medical, robotics, virtual reality, and human–computer interaction, to assess hand motor skills [...] Read more.
The sensory glove (also known as data or instrumented glove) plays a key role in measuring and tracking hand dexterity. It has been adopted in a variety of different domains, including medical, robotics, virtual reality, and human–computer interaction, to assess hand motor skills and to improve control accuracy. However, no particular technology has been established as the most suitable for all domains, so that different sensory gloves have been developed, adopting different sensors mainly based on optic, electric, magnetic, or mechanical properties. This work investigates the performances of the MANUS Quantum sensory glove that sources an electromagnetic field and measures its changing value at the fingertips during fingers’ flexion. Its performance is determined in terms of measurement repeatability, reproducibility, and reliability during both quasi-static and dynamic hand motor tests. Full article
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46 pages, 4133 KB  
Review
Flux-Weakening Control Methods for Permanent Magnet Synchronous Machines in Electric Vehicles at High Speed
by Samer Alwaqfi, Mohamad Alzayed and Hicham Chaoui
Electronics 2025, 14(19), 3779; https://doi.org/10.3390/electronics14193779 - 24 Sep 2025
Viewed by 41
Abstract
Permanent magnet synchronous motors (PMSMs) are widely favored by manufacturers for use in electric vehicles (EVs) because of their many benefits, which include high power density at high speeds, ruggedness, potential for high efficiency, and reduced control complexity. However, since the Back Electromotive [...] Read more.
Permanent magnet synchronous motors (PMSMs) are widely favored by manufacturers for use in electric vehicles (EVs) because of their many benefits, which include high power density at high speeds, ruggedness, potential for high efficiency, and reduced control complexity. However, since the Back Electromotive Force (EMF) increases proportionally with the motor’s rotational speed, it must be carefully controlled at high speeds. Flux-weakening (FW) control is required to avoid excessive electromagnetic flux beyond the power source and inverter’s voltage restrictions. This paper aims to compare various FW control strategies and analyze their effectiveness in maximizing the speed of PMSMs in EV applications while ensuring stable and reliable performance. Various FW approaches, such as voltage-based control, current-based control, and advanced predictive control methods, are examined to determine how each method balances speed enhancement with torque output and efficiency. In addition, other control strategies are crucial for optimizing the performance of PMSMs in electric vehicles. Among the most popular methods for controlling torque and speed in PMSMs are Field-Oriented Control (FOC), Direct Torque Control (DTC), and Vector Current Control (VCC). Each control technique has advantages and is frequently cited in the literature as a crucial instrument for improving EV motor control. This article provides a comprehensive evaluation of FW methods, highlighting their respective advantages and disadvantages by synthesizing the findings of numerous studies. In addition to outlining future research directions in FW control for EV applications, this study provides essential insights and valuable suggestions to help select FW control techniques for various PMSM types and operating conditions. Full article
(This article belongs to the Special Issue Advanced Control and Power Electronics for Electric Vehicles)
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16 pages, 1482 KB  
Article
Room Temperature Synthesis of a Novel Quinolinoxazine, Polymerization and Flammability Studies
by Maria Laura Salum, Daniela Iguchi, Carlos Rodriguez Arza, Nora Pellegri, Hatsuo Ishida and Pablo Froimowicz
Polymers 2025, 17(18), 2546; https://doi.org/10.3390/polym17182546 - 20 Sep 2025
Viewed by 148
Abstract
A novel quinoline-containing benzoxazine resin, 8HQ-fa, has been successfully synthesized at room temperature using sustainable raw materials, such as 8-hydroxyquinoline and furfurylamine as the phenol and amine source, respectively. The chemical structure of the hereinafter referred to as quinolinoxazine is fully characterized [...] Read more.
A novel quinoline-containing benzoxazine resin, 8HQ-fa, has been successfully synthesized at room temperature using sustainable raw materials, such as 8-hydroxyquinoline and furfurylamine as the phenol and amine source, respectively. The chemical structure of the hereinafter referred to as quinolinoxazine is fully characterized by Fourier transform infrared spectroscopy (FT-IR), 1H and 13C nuclear magnetic resonance spectroscopy (NMR), as well as by 2D 1H–1H nuclear Overhauser effect spectroscopy (NOESY) and 1H–13C heteronuclear multiple quantum correlation (HMQC) NMR. Thermal properties and polymerization behavior of the monomer are studied by differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA). The resulting polymer is also characterized in terms of its thermal and fire-related properties by DSC, TGA, and microscale combustion calorimetry (MCC). The resulting thermoset, poly(8HQ-fa), presents good thermal stability as evidenced by its Tg (201 °C), Td5 and Td10 (307 and 351 °C, respectively), and char yield (42%), and low flammability as determined by the LOI, heat release capacity, and total heat released values (34.3, 143 J/gK, and 10.8 kJ/g, respectively), making it a self-extinguishing thermoset. The combination of properties and advantages in the synthesis of 8HQ-fa, accompanied by a low polymerization temperature, suggests its great potential in the field of high-performance polymers. Full article
(This article belongs to the Section Polymer Chemistry)
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9 pages, 3185 KB  
Article
Magnetic Purcell Enhancement by Plasmon-Induced Magnetic Anapole Mode in the Gap of Oblate Nano-Ellipsoid on Metal Mirror Structure
by Yafei Li, Jiani Li, Zhuangzhuang Xu, Xiufei Li, Songda Gu, Ze Li and Meng Wang
Nanomaterials 2025, 15(18), 1451; https://doi.org/10.3390/nano15181451 - 20 Sep 2025
Viewed by 229
Abstract
Magnetic anapole states associated with the destructive interference between magnetic dipole and magnetic toroidal moments result in suppressed scattering accompanied by strongly enhanced near fields. Here, we demonstrate the existence of such modes in the gap of a gold oblate nano-ellipsoid on gold [...] Read more.
Magnetic anapole states associated with the destructive interference between magnetic dipole and magnetic toroidal moments result in suppressed scattering accompanied by strongly enhanced near fields. Here, we demonstrate the existence of such modes in the gap of a gold oblate nano-ellipsoid on gold mirror (ONEOM) structures and observe a pronounced Purcell factor enhancement for magnetic dipole radiation upon introducing magnetic dipoles into the gap. We systematically investigate the dependence of the magnetic radiation Purcell factor on gap size and structural parameters. Notably, a 230-fold Purcell factor enhancement is achieved for the ONEOM configuration. This result highlights the potential of ONEOM structures in applications requiring efficient magnetic dipole emission, including nonlinear frequency conversion, plasmonic sensing, and single-photon sources. Full article
(This article belongs to the Special Issue Theoretical Calculation Study of Nanomaterials: 2nd Edition)
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19 pages, 649 KB  
Article
Study on the Gamma-Ray Radiation Properties of High-Redshift Blazars at z > 2.5
by Fan Wu, Li Zhang and Benzhong Dai
Universe 2025, 11(9), 320; https://doi.org/10.3390/universe11090320 - 18 Sep 2025
Viewed by 138
Abstract
The high-redshift blazars are important cosmological probes for exploring the early universe and unraveling the fundamental emission processes and the structure of the active galactic nuclei. The high-energy GeV gamma-ray emissions of 38 high-redshift blazars (z > 2.5) observed by Fermi-LAT were analyzed. [...] Read more.
The high-redshift blazars are important cosmological probes for exploring the early universe and unraveling the fundamental emission processes and the structure of the active galactic nuclei. The high-energy GeV gamma-ray emissions of 38 high-redshift blazars (z > 2.5) observed by Fermi-LAT were analyzed. Along with the Archive multiwavelength data, we employ one-zone leptonic external Compton (EC) models to reproduce the spectral energy distributions (SEDs) of 38 sources. Both the external photons from the molecular torus (MT) and the broad-line region (BLR) are considered. We obtained the best-fitting parameters for describing the characteristics of the jets and accretion disks. The results indicate that high-redshift blazars exhibit higher γ-ray luminosities, energy densities, jet powers, kinetic powers, accretion disk luminosities, black hole (BH) masses, radiation efficiencies, and mass accretion rates compared to low-redshift blazars. For high-redshift blazars, the influence of the accretion rate on jet power appears to weaken, and in most cases, the jet power exceeds the total accretion power. We speculate that for high-redshift blazars, rapid accretion may lead to magnetic field saturation, thereby reducing the effectiveness of the Blandford–Payne (BP) process. Consequently, the Blandford–Znajek (BZ) process is likely to play a more dominant role in powering jets in high-redshift blazars compared to low-redshift blazars. Naturally, we acknowledge that selection effects cannot be fully eliminated. Full article
(This article belongs to the Special Issue Multi-wavelength Properties of Active Galactic Nuclei)
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15 pages, 5479 KB  
Article
Transfer Hydrogenation of Vanillin with Formic Acid over Graphene-Encapsulated Nitrogen-Doped Bimetallic Magnetic Pd/Fe@N/C Catalyst
by Hualiang Zuo, Yulong Lei and Jianguo Liu
Catalysts 2025, 15(9), 906; https://doi.org/10.3390/catal15090906 - 18 Sep 2025
Viewed by 204
Abstract
The improved biomass-derived aldehyde compounds represent a valuable route to the production of high-value-added fuels and chemicals. However, the majority of mature catalytic systems exhibit low hydrodeoxygenation (HDO) activity, even under harsh reaction conditions. In this study, it was observed that a Pd/Fe [...] Read more.
The improved biomass-derived aldehyde compounds represent a valuable route to the production of high-value-added fuels and chemicals. However, the majority of mature catalytic systems exhibit low hydrodeoxygenation (HDO) activity, even under harsh reaction conditions. In this study, it was observed that a Pd/Fe magnetic bimetallic catalyst, in conjunction with formic acid (FA) as a hydrogen source and nitrogen-containing carbon material as a support, exhibited remarkable catalytic performance for the conversion of phenyl aldehydes in oxygenates derived from crude lignin. In the hydrogenation of vanillin, the Pd/Fe@N/C catalyst demonstrated superior catalytic activity under mild reaction conditions of 80 °C. When ethyl acetate was used as the solvent, the product was vanillyl alcohol (VA), and when cyclohexane was employed as the solvent, the product was p-methyl guaiacol (MMP). The yields achieved were 84.5% and 92.3%, respectively. It is recommended that further exploration of the FLOW reactor system be considered at a later stage due to the magnetic and easily separable characteristics of the catalyst. The excellent mass transfer and heat transfer performance of the FLOW reactor system will further ensure that the reaction conditions are moderate and will strive to achieve normal-temperature conversion. Full article
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16 pages, 1937 KB  
Article
Aptamer-Functionalized Magnetic Nanoparticles for Rapid Isolation of Environmental Escherichia coli
by Zulema Herazo-Romero, Wendy Yulieth Royero-Bermeo, Miguel Octavio Pérez-Navarro, Miryan Margot Sánchez-Jiménez and Juan David Ospina-Villa
Environments 2025, 12(9), 329; https://doi.org/10.3390/environments12090329 - 18 Sep 2025
Viewed by 432
Abstract
Access to safe water remains a vital public health challenge, especially in low- and middle-income countries like Colombia, where untreated sources lead to severe diarrheal diseases in children under five. Escherichia coli (E. coli), a key indicator of fecal contamination, is [...] Read more.
Access to safe water remains a vital public health challenge, especially in low- and middle-income countries like Colombia, where untreated sources lead to severe diarrheal diseases in children under five. Escherichia coli (E. coli), a key indicator of fecal contamination, is often detected using culture-based methods that are time-consuming and rely on specialized infrastructure. To overcome these limitations, we developed an aptamer-based isolation system targeting environmental E. coli. Aptamers were obtained using a Cell-SELEX protocol, and after six enrichment rounds, two candidates—APT-EC-1 and its truncated version APT-EC-MUT—were synthesized and attached to carboxyl-functionalized magnetic nanoparticles (MNP-COOH). Both complexes demonstrated a strong binding affinity and high specificity, successfully isolating E. coli from environmental and ATCC reference strains in the laboratory. Sensitivity tests detected E. coli at dilutions up to 1:10,000, showing reliable performance. In early in-field testing with environmental water samples, APT-EC-1 consistently identified E. coli colonies, while APT-EC-MUT struggled with low bacterial levels, illustrating performance differences. These findings demonstrate the promise of aptamer-functionalized MNPs as the basis for quick, affordable, and portable biosensors for water quality testing, especially in resource-scarce areas. Future efforts will add colorimetric or electrochemical readouts to allow real-time, on-site detection of fecal contamination. Full article
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