Search Results (254)

Containerized ornamental plant production requires efficient irrigation strategies to balance plant quality with water and nutrient conservation. This study evaluated four leaching fraction (LF) levels (0%, 20%, 40%, and 60%) in a completely randomized design with three replications, each consisting of three pots, to determine their effects on plant growth, ornamental quality, gas exchange, water use efficiency (WUE), and macronutrient leaching in off-season potted Curcuma cv. ‘Jasmine Pink’. Irrigation volumes were determined using crop coefficient (Kc)-based estimates derived from evaporation pan measurements. The results showed that the highest LF level (60%) significantly improved several ornamental quality traits, including flower number per cluster, leaf greenness, specific leaf area, and compactness index, while also increasing aerial dry weight and improving gas exchange parameters during the flowering stage. These improvements were associated with reduced substrate electrical conductivity, indicating lower soluble salt accumulation in the root zone under higher LF treatments and more favorable conditions for plant growth. Leaching fraction is commonly used in containerized crop production to prevent excessive salt accumulation in the root zone by allowing excess irrigation water to drain from the substrate. However, increasing LF also resulted in greater irrigation water consumption and higher macronutrient losses through leachate, particularly potassium. In contrast, lower LF treatments (0–20%) improved water use efficiency and reduced nutrient losses but were associated with higher substrate electrical conductivity, suggesting greater soluble salt accumulation in the root zone. Overall, the results indicate that a higher LF (60%) provided the greatest improvement in plant growth and ornamental quality under the conditions of the present study for off-season potted Curcuma alismatifolia production, although integrated strategies may be required to reduce water and nutrient losses. These findings provide practical insights for optimizing irrigation management in container-grown ornamental crops. Full article

Type 2 diabetes mellitus is a relevant cardio–renal–metabolic disorder in which chronic low-grade inflammation and oxidative stress have a crucial function in linking insulin resistance, endothelial dysfunction, β-cell impairment, and progressive organ injury. In this context, nutrition has emerged as a key modifiable determinant of metabolic homeostasis, capable of influencing inflammatory signalling, redox balance, mitochondrial function, and gut microbiota–host interactions. The objective of this review is to critically summarise the mechanistic connections among inflammation, oxidative stress, and diabetes progression, and to investigate how dietary factors and patterns, as well as nutrition-responsive biomarkers, influence these pathways and their cardiorenal consequences. We discuss the effects of macronutrient quality, dietary fibre, fatty acids, polyphenols, and specific micronutrients, including vitamin C, vitamin E, selenium, zinc, and magnesium, as well as the role of Mediterranean, DASH, and plant-based diets in improving glycaemic control, endothelial function, and cardio-renal risk profiles. We also summarise established and emerging biomarkers of inflammation and oxidative stress that may improve risk stratification and the evaluation of nutrition-based interventions. Overall, current evidence supports a shift from a purely glucose-centred approach toward an integrated model in which dietary modulation of inflammatory and oxidative pathways helps reduce cardiovascular and renal risk. However, heterogeneity of interventions, variability in biomarker assessment, and interindividual differences in dietary response represent major limitations. Future research should focus on biomarker-informed, precision-oriented nutritional approaches integrated within contemporary cardio–renal–metabolic care. Full article

This study aimed to examine the nutrient intake in Polish middle-distance runners. The secondary objective was to examine the biochemical profile and antioxidant status in the blood serum of the runners, and then compare to the control group. The research was conducted among 44 volunteers. Information regarding participants’ diet was gathered for three days before the study took place. Also, blood serum of athletes and control subjects was examined to obtain information about their antioxidant status, biochemical parameters, and micro- and macronutrients. This study showed that runners consumed more protein, sodium, zinc, phosphorus, water, vitamin A and E than the control group. The daily intake of selected vitamins, micro- and macronutrients in a group of athletes is approximately two times higher than in the control group. The control group consumed more polyphenols compared to the runners group (p < 0.05). The consumption of phenols was almost two times lower among runners compared to non-runners. The higher uric acid concentration (p = 0.025) and lower chloride concentration (p = 0.011) were observed in the blood serum of runners compared to the control group. The diet of middle-distance runners is well-balanced but need some modifications, especially regarding the intake of vitamin E, folates and polyphenols. Full article

Chlorella Growth Factor (CGF) is a nucleotide-rich, water-soluble intracellular fraction derived from disrupted Chlorella biomass that has historically been described as a “growth-promoting” extract but remains poorly defined at the molecular level. In this review, we propose that CGF should not be interpreted as a classical receptor-binding growth factor, but rather as a heterogeneous, nucleotide-dominant metabolic fraction that may modulate cellular redox balance and biosynthetic capacity. We integrate available evidence on CGF characterization, including A260-based analytical indices, mineral-dependent biosynthesis, and extraction methodologies, with mechanistic observations from in vitro, animal, and applied biological systems. Across these contexts, CGF-associated fractions have been reported to influence redox-sensitive pathways, including NAD(H)/NADP(H)-linked processes, MAPK/AP-1 signaling, extracellular matrix regulation, and humoral immune responses. However, most mechanistic evidence remains indirect, and compositional heterogeneity limits direct comparability across studies. From a nutritional perspective, CGF contributes minimal macronutrient value but may provide conditionally relevant dietary nucleotides, amino acids, and redox-active metabolites that support metabolic processes under stress conditions. Observed biological effects are consistent with a model of metabolic permissiveness, in which CGF-associated fractions may support endogenous cellular functions rather than directly initiating signaling cascades. Key translational challenges include the lack of compositional standardization, limited nucleotide speciation, variability in extraction protocols, and the absence of pharmacokinetic and controlled human studies using well-characterized CGF preparations. Overall, CGF may be conceptualized as a candidate dietary bioactive with redox-centered and metabolically permissive properties. Further work integrating standardized analytical frameworks with mechanistic and clinical validation will be required to establish its role in human nutrition and functional food applications. Full article

Modern genetic selection for high productivity has created a physiological conflict in ruminants, where the metabolic demands of lactation compete directly with the energy requirements of reproduction. This review provides a mechanistic synthesis of how key nutritional factors modulate the endocrine and cellular pathways governing reproductive success in cattle and sheep. Negative energy balance (NEB), characteristic of the early postpartum period, suppresses the hypothalamic–pituitary–gonadal (HPG) axis by impairing the pulsatile secretion of gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH), mediated through reduced kisspeptin signaling, growth hormone (GH) resistance, and decreased circulating insulin, insulin-like growth factor-1 (IGF-1), and leptin. At the macronutrient level, excess rumen-degradable protein elevates blood urea nitrogen and impairs the uterine environment, while omega-3 polyunsaturated fatty acids inhibit prostaglandin F2α synthesis to support corpus luteum maintenance. At the micronutrient level, selenium, copper, and zinc are essential antioxidant cofactors protecting gametes and embryos from oxidative stress, while vitamins A, D, and E regulate gene expression in reproductive tissues. Furthermore, maternal nutrition during critical gestational windows programs the reproductive capacity of offspring through epigenetic modifications, with profound implications for long-term herd fertility. Understanding these nutritional–reproductive interactions is crucial for developing precision feeding strategies that optimize herd fertility, improve animal welfare, and ensure the economic sustainability of livestock management. A thorough understanding of these nutritional–reproductive interactions is essential for developing precision feeding strategies that optimize fertility in high-producing ruminants. Full article

The menstrual cycle represents a dynamic infradian rhythm characterized by coordinated fluctuations in ovarian steroids that extend beyond reproductive function and influence systemic metabolism. This narrative review synthesizes current evidence on how menstrual cycle phase modulates energy balance, macronutrient metabolism, micronutrient handling, and responses to dietary bioactive compounds. Across phases, small-to-moderate but consistent differences emerge in energy intake, resting energy expenditure, substrate utilization, and protein turnover, with a tendency toward increased energy intake and lipid oxidation during the mid-luteal phase compared with the early follicular and peri-ovulatory phases. Emerging metabolomics data further reveal coordinated cyclical variation in amino acids, B vitamins, and lipid species, suggesting temporally sensitive windows in which low energy availability or micronutrient insufficiency may more readily impair performance, recovery, or symptom burden. Importantly, menstrual cycle-related metabolic variability reflects not only estradiol and progesterone oscillations but also integrated adaptations across the hypothalamic–pituitary–adrenal axis, autonomic nervous system, immune signaling, and gut microbiota. These interconnected systems contribute to inter- and intra-individual heterogeneity in metabolic phenotype. From a clinical and applied perspective, the evidence supports “cycle-aware” but non-dogmatic nutritional strategies, particularly in contexts of metabolic dysfunction, high training loads, or reproductive disorders. Future research should systematically verify cycle phase, incorporate multi-system biomarkers, and adopt sex-specific analytical frameworks to improve translational relevance. Recognizing the menstrual cycle as a biologically meaningful metabolic variable may enhance precision nutrition, exercise prescription, and metabolic risk assessment in women. Full article

Background: Table olives are key elements of Mediterranean cuisine, yet their contribution within traditional Greek culinary preparations remains underexplored. Aim: This study evaluated the nutritional composition of 70 Greek recipes (appetizers, salads, and main courses) incorporating table olives. Methods: We conducted nutritional analysis based on a previous study, integrating the USDA food composition database and the official Greek food composition tables, yield and retention factors, and standardized portion measures. Energy content was assessed against cut-off points for nutritionally balanced meals. Principal component analysis (PCA) and ternary plots were applied to examine the relationship between macronutrients, energy, and fatty acid profiles. Results: Mean energy density was 154.5 kcal/100 g, with fat as the dominant macronutrient (11.0 g/100 g), primarily monounsaturated. Proteins, carbohydrates, sugars, and dietary fiber contributed less to total energy, and fiber levels were moderate (1.24 g/100 g). Conclusions: These findings highlight that traditional olive-based recipes deliver energy predominantly through fat-rich ingredients, mainly monounsaturated fatty acids. The study underscores the need for portion awareness and potential recipe adjustments to enhance nutritional balance and offers a framework for assessing the dietary value of Mediterranean culinary traditions. Full article

Within the project “Small-scale grants for biodiversity actors in South-East Europe 2023–2025”, whose main mission is the collection of local varieties and populations, a fundamental question arises: “Why are ‘ancestral’ maize varieties and populations still cultivated?” To answer this question, we conducted a comprehensive set of investigations on 14 maize (Zea mays L.) varieties and populations and on one hybrid, collected from the historical regions of Romania—Transylvania, Moldova, Oltenia and Muntenia. The studies combine quantitative, qualitative and computational methods and focused on energy consumption associated with maize cultivation; maize grain production and related agronomic characteristics; the content of energy macronutrients (protein, fat, starch) and energy elements (CHNS-O, ash, moisture); and nutritional and thermal energy values (upper and lower) of whole cornmeal. The sustainability of the cultivation of local maize varieties was also evaluated based on the energy balance and the energy efficiency ratio. The results demonstrated that the cultivation of “ancestral” maize varieties and populations is sustainable, because the amount of energy obtained, expressed in kJ ha⁻¹, as nutritional energy (24,740,195.04–90,287,743.07), higher heating energy (55,162,983.798–193,374,572.55) and lower heating energy (32,329,465.37–113,906,753.63), is greater than the amount of energy consumed for the establishment and maintenance of these crops (1,742,798.75–19,524,555.05). Full article

Phosphorus (P) is an essential macronutrient for plant growth and a major limiting factor for crop productivity, especially in tropical soils characterized by low P availability and high fixation capacity. The strong dependence of modern agriculture on non-renewable phosphate fertilizers, combined with their low use efficiency, raises economic and environmental concerns and reinforces the need to improve phosphorus use efficiency (PUE) in maize. PUE is a complex trait governed by integrated morphophysiological, biochemical, and molecular mechanisms related to phosphorus acquisition, internal remobilization, metabolic reprogramming, and root system plasticity. Recent advances using omics-based approaches have substantially expanded the understanding of these mechanisms, revealing coordinated regulation of carbon and energy metabolism, phosphatase activity, redox balance, and root meristem dynamics under P-limiting conditions. In parallel, increasing evidence demonstrates the important role of phosphate-solubilizing and plant growth-promoting bacteria in enhancing P availability through organic acid secretion, enzymatic mineralization of organic P forms, and modulation of root architecture. However, despite these advances, the genetic basis of plant responsiveness to beneficial bacteria and the interaction between host genotype and microbial activity remain poorly explored. This review integrates current knowledge on phosphorus dynamics in the soil–plant system, the genetic control of PUE in maize, and the contribution of beneficial bacteria, highlighting the importance of combining classical breeding, molecular approaches, and microbial strategies to accelerate the development of maize cultivars with improved phosphorus efficiency and reduced fertilizer dependency. Full article

Nitrogen (N) is a key macronutrient that influences the uptake and partitioning of other essential elements in plants. In this research, we evaluated the effect of different N concentrations in the nutrient solution (0, 4.2, 8.4, and 12.6 mg L⁻¹) during the flowering stage on the concentration and accumulation of macronutrients in organs of Mexican marigold (Tagetes erecta L.) ‘Inca’. After 40 days of treatment, plants were separated into leaves, flowers, stems, and roots to determine the concentrations of N, P, K, Ca, Mg, and S, as well as their accumulation based on dry biomass. Nitrogen supply significantly affected dry biomass production and its partitioning among organs, promoting biomass allocation to leaves and flowers while reducing relative root biomass at higher N concentrations. Nitrogen concentrations and accumulation increased in leaves, stems, and flowers as N supply increased, whereas an inverse relationship was observed in roots. When applying 8.4 and 12.6 mg N L⁻¹, phosphorus displayed enhanced concentrations in leaves and stems, although root tissues did not change the concentration of this nutrient. When N was supplied at up to 8.4 mg L⁻¹, the concentration of potassium rose in aboveground organs but decreased at the highest dose, while its accumulation in roots was reduced under high N concentrations tested. Calcium exhibited greater accumulation in the aboveground organs, particularly at 12.6 mg N L⁻¹. Magnesium concentration and accumulation increased in aboveground organs with increasing N supply, whereas its accumulation in roots decreased. The highest concentrations of sulfur in leaves and stems were observed at 8.4 mg N L⁻¹, and its accumulation in the aboveground organs tended to stabilize at the highest dose. Effect size analysis (partial ηp²) revealed that N supply explained a large proportion of the variance in macronutrient concentration and accumulation in aerial organs, whereas responses in roots were generally weaker and nutrient specific. Overall, our data indicate that intermediate N levels (8.4 mg L⁻¹) boost a more efficient nutritional balance in the aboveground organs, while the highest dose predominantly enhances Ca and Mg accumulation. Understanding how these plants respond to nitrogen can help improve the quality of Mexican marigold crops and make better use of fertilizers. Full article

Background/Objectives: The menopausal decline in estrogen levels accelerates age-related changes, including visceral adiposity, insulin resistance, sarcopenia, osteoporosis, and endothelial dysfunction. While nutrition independently influences these outcomes, the interactive role of estrogen signaling and nutrient metabolism in healthy aging remains underexplored. This article evaluates these interactions. Methods: We conducted a narrative synthesis of studies examining estrogen’s effects on energy balance, adipose regulation, muscle, bone, and cardiovascular health, with an emphasis on estrogen-like nutritional modulators and phytoestrogens. In addition, we present original experimental data from our laboratory investigating sex-specific vascular responses to G protein-coupled estrogen receptor (GPER) activation using functional myography in isolated rat aortic rings from young adult and middle-aged rats (n = 6–8 per group) to assess responses to the GPER agonist G-1 (1.0 μM). Results: Literature evidence demonstrates that estrogen supports macronutrient utilization, suppresses adipose inflammation, preserves bone density, and promotes endothelial function. Phytoestrogens may engage estrogen-responsive pathways to mitigate age-related physiological decline. Our original findings show that GPER agonism enhances both contractile and vasodilatory responses in female (p < 0.05) but not male rat aortas, providing mechanistic evidence of sex-specific vascular estrogen signaling. These results suggest that dietary phytoestrogens and nutrient-rich dietary patterns may, in part, activate GPER-dependent pathways to support cardiovascular resilience in aging women. Conclusions: Estrogen–nutrition interactions are central to metabolic adaptation and healthy aging. Our findings highlight GPER as a functionally resilient pathway in aging vasculature, offering a putative mechanistic link for nutritional modulation. However, direct translation of these findings to human clinical outcomes remains to be established. Precision nutrition strategies targeting GPER represent a promising framework for healthy aging, though large-scale human trials are necessary to confirm these receptor-specific effects. Full article

The progressive decline in fertility in nutrient-poor sandy soils has increased interest in soil conditioners that improve nutrient availability. Rising costs and the need to restore biological fertility have shifted attention towards fertilizers that not only enhance productivity but also improve soil biological activity. This study aims to evaluate the effects of biochar (3% w/w; BC) and wood distillate (one irrigation intervention per week at 2% v/v; WD), applied individually or in combination (BC + WD), on a nutrient-poor soil, evaluating soil fertility and basil plant physiology and growth but also antioxidant responses in a pot experiment. Soil NPK content and enzymatic activity were assessed, while plant growth, macronutrient uptake, gas exchange, and antioxidant system responses were monitored after 28 and 56 days of treatment. BC treatment, followed by BC + WD treatment, increased soil P availability by 36% and 37%, respectively, after 56 days compared to untreated soil (CNT). A similar pattern was evidenced for the exchangeable K and pH of the soil. Although BC led to a reduction in soil enzymatic activity, the BC + WD treatment enhanced urease and acid phosphatase activity after 56 days by 26% and 7%, respectively, compared to CNT. Similarly, P uptake by plants was improved by BC + WD after 56 days, while potassium, K, uptake increased in both the BC and BC + WD treatments by 38% and 75% at the final sampling. BC or BC + WD resulted in improved photosynthesis and gas exchange, while WD influenced responses related to redox balance and antioxidant activity over time. Moreover, BC + WD slightly stimulated an increase in dehydroascorbate reductase (+52%), ascorbate peroxidase (+78%), and glutathione reductase (+41%) activity compared to CNT, enforcing the plant antioxidant system. Therefore, the positive antioxidant responses were primarily attributed to the use of BC rather than WD. Both BC and BC + WD proved to be effective and sustainable soil conditioners with beneficial effects on soil P and K availability, as well as certain enzymatic activities. For plants, the effects were more pronounced with BC treatment, showing antioxidant responses within the first 56 days. In general, BC improved soil fertility, and WD acted as a modulator of nutrient dynamics and plant physiological responses, especially when combined with BC. Full article

Modern agriculture requires optimizing available resources to maximize production while minimizing environmental impact without increasing economic costs. Hydroponic agriculture replaces soil with inert media that provide physical support for plants but do not supply nutrients. In this type of agricultural production, fertilization with nutrient solutions is essential, as they supply the 15 elements necessary for proper plant development. These solutions consist of mixtures of different amounts of fertilizers dissolved in water. In this context, a method based on a simulated annealing algorithm is proposed, a metaheuristic that optimizes fertilizer quantities in grams to achieve target concentrations in parts per million for six macronutrients and nine micronutrients. The algorithm addresses a multi-objective optimization problem, balancing two competing goals: first, maximizing the accuracy of the fertilizer balance to achieve the required nutritional levels, and second, minimizing the total cost of the fertilizer mixture. The algorithm’s fitness function weights the total cost of the fertilizers used and the total relative error between the concentrations obtained and those desired, allowing the relative importance of cost and accuracy in the nutrient solution to be adjusted. The results of three experiments with varying nutrient levels are presented for a 1000-L water tank. The first experiment consisted of three macronutrients and two micronutrients. The second configuration added three macronutrients and two micronutrients, for a total of ten nutrients. Finally, five micronutrients were added to complete the 15 essential nutrients for plants. It is important to note that there are several methods for calculating micronutrients that contribute to precision agriculture, increasing the complexity of finding a solution that meets established nutritional requirements. The nutrient concentrations in parts per million required for tomato cultivation during the vegetative development stage. To balance nutrient accuracy and solution cost, we applied weighting factors of $0.65,0.75,0.85,0.90,0.95,$ and $1.0$ for accuracy. The corresponding weights for cost were calculated as the complement of these values (totaling 1). By favoring nutrient accuracy with a weighting of 1, accuracies of 0.00500, 0.02618, and 0.03077 parts per million were achieved in each experiment, respectively. Meanwhile, the lowest cost is $2.06$, $2.72$, and $2.70$ USD for the aforementioned experiments. Full article

Although a balanced supply of macronutrients is essential for apple tree growth and orchard productivity, the relationship between macronutrient uptake and partitioning in the entire apple tree remains ambiguous. To address this gap, a 2-year field experiment was conducted from 2019 to 2021 in a newly established dwarf ‘Fuji’ apple orchard in Shaanxi, one of the main apple production areas in China. The results showed that the annual uptake was 11.2−15.0 kg ha^–1 for calcium, 1.5−1.9 kg ha^–1 for magnesium, and 1.0 kg ha^–1 for sulfur. During the 2019–2020 season, trees absorbed most of the calcium, magnesium, and sulfur from the end of spring shoot growth to nutrient withdrawal, accounting for 70.8%, 76.7%, and 80.0% of the annual calcium, magnesium, and sulfur uptake, respectively. During the 2020–2021 season, 57.7%, 61.6%, and 45.5% of the annual calcium, magnesium, and sulfur uptake occurred from the slow growth of the spring shoot to the end of spring shoot growth, respectively. The ratio of nitrogen, phosphorus, potassium, calcium, magnesium, and sulfur absorbed by the tree was 1:0.17:0.82:1.1:0.14:0.092 during the first season, and during the second season, it was 1:0.18:0.60:1.7:0.21:0.11. Regarding the accumulation and partitioning of macronutrients in different organs, calcium accumulation and partitioning were higher than those of the other nutrients in trunks. In coarse roots, branches, and shoots, calcium accumulation was also higher compared to other nutrients. In fine roots, nitrogen accumulation was slightly higher than calcium. In leaves, nitrogen accumulation was higher than the other nutrients, whereas in fruits, potassium accumulation and partitioning were higher than those of the other nutrients. These findings reveal distinct macronutrient requirement patterns across the whole apple tree and specific organs, providing new insights into maintaining nutrient homeostasis in apple trees and optimizing nutrient resource allocation for efficient orchard production. Full article

Improving crop productivity largely depends on understanding soil fertility constraints and the effects of nutrient management on yield performance. Accurate determination of existing soil nutrient status and targeted application of limiting nutrients are essential for enhancing wheat (Triticum spp.) productivity. However, the specific effects of omitting one of the macronutrients such as nitrogen (N), phosphorus (P), or potassium (K) on wheat yield have not been investigated in the target area. This study employed the Quantitative Evaluation of the Fertility of Tropical Soils (QUEFTS) model to estimate the N, P, and K fertilizer requirements needed to achieve a predefined wheat yield target. The objectives were to: (i) evaluate yield responses to complete versus nutrient omission (N, P, or K) fertilization treatments, and (ii) analyze corresponding nutrient uptake and use efficiency dynamics. The experimental treatments included: (1) full NPK fertilization, (2) NP only (K omitted), (3) NK only (P omitted), (4) PK only (N omitted), and (5) an unfertilized control. Topsoil samples were analyzed and used as inputs for the QUEFTS model. Yield and agronomic data, as well as nutrient uptake and use efficiency, were measured. Model performance was validated using standard statistical metrics. Results showed that full NPK application significantly (p < 0.05) improved yield, yield components, and nutrient uptake compared to omission treatments and the control. The strong agreement between QUEFTS-predicted and observed yields highlights the model’s potential as a reliable, cost-effective decision-support tool for optimizing site-specific fertilizer recommendations. These findings demonstrate that balanced NPK fertilization markedly boosts wheat yield and nutrient uptake, while the QUEFTS model provides a powerful, reliable tool for tailoring fertilizer management to local soil conditions. Full article