Search Results (281)

Dietary supplementation with sodium butyrate or bovine colostrum modulates the gut–liver axis in weaned piglets. Sodium butyrate exerted beneficial effects on liver function and lipid parameters, while also inhibiting inflammation and promoting the maintenance of the intestinal barrier. A particularly pronounced effect was observed with bovine colostrum supplementation, which significantly increased average daily weight gain (p < 0.001). In addition, piglets receiving colostrum consumed more feed and exhibited a significantly lower feed conversion ratio (p = 0.002). Metabolic changes induced by sodium butyrate and bovine colostrum supplementation resulted in alterations in the hepatic fatty acid profile, including a reduction in n-3 polyunsaturated fatty acids and a decrease in collagen fiber content in the liver (p = 0.03). The nutritional interventions did not significantly affect microbial diversity indices; however, marked changes in volatile fatty acid concentrations were observed in the large intestine. These changes indicate enhanced microbial fermentation and increased nutrient absorption in the experimental groups. Significant increases were detected in acetic acid (p = 0.003) as well as in butyric, isobutyric, and valeric acids (p = 0.014, p = 0.024, and p = 0.038, respectively). Supplementation with sodium butyrate and dried bovine colostrum also led to increased hepatic concentrations of macro- and microelements in piglets from the experimental groups. Genomic analyses suggest that sodium butyrate modulates hepatic metabolic and inflammatory pathways by downregulating PPAR (peroxisome proliferator-activated receptor) and SIRT3 (sirtuin 3) expression and reducing TNF (tumor necrosis factor) gene expression, highlighting its potential role in regulating lipid metabolism, oxidative stress, and inflammation in a porcine model. Overall, the results indicate that both supplements may contribute to the modulation of gut microbial activity and liver metabolism in weaned piglets. Full article

This study investigated the macro- and microelement profiles and bioactive substances (BAS) in the herbaceous species of the genus Rheum collected from two villages near Almaty, Kazakhstan, and the accumulation of heavy metals (HMs) in wild plants of Eastern Kazakhstan. Representative zonal species from the steppes and desert–steppes (Eastern Kazakhstan) were analyzed—totaling one hundred samples across 18 species from six families. According to the research, different plant species have a selective ability to accumulate heavy metals, even when growing in the same type of soil. Long-term observations reveal dominant patterns of HM (Cu, Zn, Mn, Co, Pb, Cd) occurrence in dry-steppe vegetation. It was found that Cu and Co demonstrate moderate bioadsorption with minimal accumulation, while Zn, Mn, and Pb show strong biological enrichment. Cadmium falls under elements with pronounced biological retention. Copper and cobalt are classified as moderately absorbed with limited buildup. Native plants in this region contain substantially greater amounts of lead relative to cobalt, and reflect cadmium–zinc geochemical specificity: Cd 3.85, Zn 0.15, Mn 0.10, Pb 0.09, Co 0.07, Cu 0.04, per Clark’s concentration index. Samples of collected Rheum tataricum L. in the village of Miyaly were found to have higher levels of flavonoids, phenolic compounds, and organic acids, while raw materials from the village of Bakbakty showed increased accumulation of anthracene derivatives and tannins. Flavonoids constituted the largest proportion of the studied groups of biologically active substances in both samples, confirming the high biological and pharmacological value of the plant material studied. The results obtained during the research can be successfully applied to the development of a strategy for the conservation of plant biodiversity in the studied areas and the sustainable management of plant resources. Full article

The increasing popularity of macroalgae products highlights their potential as a safe source of essential macro and microelements for consumers. This study characterized wild and farmed macroalgae elemental composition and assessed the presence of biotoxins to identify potential health risks. Wild and farmed macroalgae samples were collected throughout 2024–2025, from three aquaculture production sites along the Portuguese coast (Ria de Aveiro, Matosinhos, and Olhão). Samples were freeze-dried, compressed and using a micro-XRF spectrometer, elemental composition was assessed. Macroalgae species factor was the strongest driver of elemental composition, explaining over 80% variation in macro and trace elements. Origin did not showcase statistical significance for elemental composition. Seasonal differences, though relatively small in extent, significantly impacted redox-sensitive elements in macroalgae, namely copper and manganese. All elements stayed below 30% of the recommended dietary recommendations, except iron (ranging from 0.5% to 111.8% of UL%) and Mn (ranging from 0.1% to 101% of UL%). All samples revealed the absence of regulated marine toxins. Only traces of the non-regulated cyclic-imine toxin SPX1 were detected in samples of Fucus vesiculosus. All quantified elements do not represent any risk for human health, strengthening the safety of macroalgae from the Portuguese coast. Full article

Peloids are natural materials widely used in balneotherapy and dermatological treatments because of their physicochemical and mineralogical properties. Despite Serbia’s long tradition of spa-based pelotherapy, comprehensive data on the chemical and radiological characteristics of local peloids remain limited. In this study, peloid samples from 13 spa locations across four regions of Serbia were systematically investigated. The aim was to determine their physicochemical properties, elemental composition, and natural radioactivity, to assess their suitability and safety for therapeutic use. The analyzed samples exhibited pronounced variability in pH (6.59–9.52), electrical conductivity (77.5–6610 μS/cm), salinity (below detection limit to 4%), and total dissolved solids, reflecting diverse geological and hydrochemical properties. Inductively coupled plasma optical emission spectrometry revealed site-specific variations in macro- and microelements, influenced primarily by local lithology and sedimentary environments, with limited indications of anthropogenic inputs. Gamma spectrometric analysis showed that the activity concentrations of naturally occurring radionuclides (²²⁶Ra, ²³²Th, ⁴⁰K, ²³⁸U, ²³⁵U, ²¹⁰Pb) were within ranges commonly reported for therapeutic muds worldwide, while anthropogenic ¹³⁷Cs was generally low. Radiological hazard indices were below internationally recommended safety limits. A preliminary screening of dermal exposure to potentially toxic elements indicated no significant noncarcinogenic risk (HI < 1) and acceptable carcinogenic risk (TCR) levels. Overall, this study provides a comprehensive chemical and radiological baseline for Serbian peloids, supporting their safe use in controlled therapeutic and wellness applications and highlighting the importance of site-specific characterization for quality assessment. Full article

The growing importance of renewable energy sources and the implementation of circular economy principles highlight the need for the rational management of biomass combustion by-products. The aim of this study was to assess the chemical composition of ash produced through the combustion of various biomass fractions from four varieties of common hazel (Corylus avellana L.) in the context of its potential for secondary use. The analysis covered the shells, husks, leaves, and shoots of the following varieties: Kataloński, Olbrzymi z Halle, Olga, and Webba Cenny. Combustion was carried out under laboratory conditions at a temperature of 550 °C, and the content of macro- and micro-element oxides (P₂O₅, K₂O, CaO, SO₃, Cl, SiO₂, MnO, Fe₂O₃, NiO, CuO) and potentially toxic elements (ZnO, TiO₂, Cr₂O₃) was determined using the EDXRF method. The results showed significant variation in the chemical composition of the ash depending on the biomass fraction and variety. The highest P₂O₅ content was found in the leaves of the Kataloński variety (5.02), whilst the highest K₂O concentration was found in the husk of the Olga variety (47.33%). The maximum CaO content was found in the leaves of the Webba Cenny variety (32.60). The leaf and husk fractions were characterised by the highest content of nutrients of fertilising importance, whilst the shells exhibited the lowest values for most macronutrients. The content of potentially toxic elements was low. The results obtained indicate that the selective utilisation of specific fractions of hazel biomass can increase the efficiency of mineral recovery whilst maintaining environmental safety, in line with the principles of the circular economy. Full article

Cowpeas are a legume widely consumed in Brazil. Given this, the objective of this study was to investigate the presence of metals (loids) in pods and leaves of Vigna unguiculata located near a highway with high vehicle traffic and a landfill, and to assess possible risks to human health. Pod and leaf samples were collected at nine points between the highway and the landfill. The elements were analyzed by inductively coupled plasma optical emission spectroscopy (ICP-OES) and quantified. The risk to human health was assessed using risk quotient and risk index values. A quantitative analysis of the chemical elements was also performed using the maximum tolerable intake level. Element concentrations were higher in the leaves than in the pods. The human health risk analysis showed that the average daily consumption of both pods (44 g/day) and leaves (67 g/day) may pose a chronic health risk to adult men and women, due to simultaneous exposure to multiple metals. It was concluded that the plant is contaminated and that its ingestion can cause toxicity, warranting warnings against cultivating areas near anthropogenic activities that may be contaminated with heavy metals, thereby affecting nutritional safety. Full article

Foliar nanofertilization is increasingly being explored as a strategy to enhance crop nutritional quality; however, dose-dependent physiological and metabolic responses remain insufficiently defined. This study evaluated the effects of conventional NPK (20:20:20) and nano-formulated NPK combined with zinc (3 and 5 g/L) on the mineral composition, bioactive compounds, antioxidant capacity, and metabolic profile of sweet pepper (Capsicum annuum L., cv. ‘Dora’) grown under controlled conditions. Physicochemical characterization of the nanofertilizer by dynamic light scattering and transmission electron microscopy confirmed nanoscale primary particle size and revealed concentration-dependent aggregation behavior at higher Zn levels. Significant differences (p < 0.05) were observed among treatments in macro- and microelement content, total phenolics, flavonoids, carotenoids, ascorbic acid, and antioxidant activity. The application of nano NPK combined with 3 g/L Zn resulted in the highest accumulation of total phenolics, flavonoids, and vitamin C, accompanied by enhanced antioxidant capacity, suggesting stimulation of secondary metabolism. In contrast, the higher Zn concentration (5 g/L) further increased carotenoid content but was associated with elevated proline levels, indicating the onset of physiological stress. Multivariate analyses (PCA and ROC) supported dose-dependent metabolic modulation and confirmed that combinations of selected metabolites contributed to clearer differentiation between fertilization regimes. Overall, the results highlight the existence of an optimal nano-zinc application range that enhances fruit functional quality while avoiding stress-related metabolic imbalance, emphasizing the importance of physicochemical stability in nano-enabled fertilization strategies. While this study focused on a single sweet pepper cultivar, future research should explore other pepper species to evaluate whether similar dose-dependent nano Zn effects are observed. Full article

Grapevine cultivar, climatic variability, and vinification practices are key determinants of wine composition. This study evaluated the physicochemical, phenolic, and elemental profiles of six wines produced from distinct cultivars cultivated in the Murfatlar Research Station (Romania) over three consecutive growing seasons (2022–2025). Red wines were obtained using differentiated maceration regimes, while white wines were produced with controlled lees contact. Total phenolic content was determined by the Folin–Ciocâlteu method, resveratrol by UHPLC-DAD, and mineral composition by ICP-MS. Linear mixed-effects models were applied to assess the effects of cultivar, vinification method, and vintage year. As expected, red wines exhibited significantly higher total phenolic and resveratrol concentrations than white wines, and maceration duration enhanced phenolic extraction. Cultivar exerted the strongest influence on physicochemical parameters and elemental composition, whereas climatic differences among vintages induced moderate but significant variations. Rare-earth elements and selected macro- and microelements exhibited consistent varietal patterns, supporting their potential as compositional markers. Toxic element concentrations remained within established regulatory limits. These findings highlight the combined influence of genetic, environmental, and technological factors on wine composition and support the integration of phenolic and mineral profiling for varietal differentiation and quality assessment. Full article

This study evaluates the impact of four drying methods—open sun drying, solar drying, infrared drying, and microwave drying—on the quality attributes and elemental retention of apricots (Prunus armeniaca L.). Experimental trials were conducted in June 2024 at the Tashkent Institute of Chemical-Technology using equal quantities of fresh apricots. Drying was continued until the moisture content, measured gravimetrically, dropped below 20% (wet basis), followed by spectroscopic analysis to determine macro- and microelement concentrations. Solar-dried apricots showed higher retention of essential nutrients in this experimental trial: potassium (2.37%), silicon (0.538%), magnesium (0.145%), calcium (0.176%), and sulfur (0.152%). In contrast, open sun drying led to significant nutrient degradation and poor visual quality. Microwave drying preserved some micronutrients but resulted in surface scorching due to uneven heating. Infrared drying yielded acceptable results but required substantial energy input. Among all methods, solar drying provided the optimal balance of high product quality and energy efficiency. The drying process required negligible electrical energy owing to exclusive reliance on solar radiation. This method supports sustainable food processing by reducing energy demand and greenhouse gas emissions while preserving nutritional quality. The results highlight solar drying as a promising, eco-friendly technique for preserving the nutritional integrity of agricultural products. These findings offer valuable scientific guidance for selecting appropriate drying technologies in the food processing industry, especially in regions with high solar potential. However, the study is limited to a single fruit variety and seasonal conditions. Full article

The elemental composition of the vitreous humor may reflect physiological and pathological processes occurring in the eye. The objective of this study was to provide a complex multielemental analysis of human vitreous humor. Vitreous humor samples (n = 57) were collected post-mortem during autopsies. Inductively coupled plasma mass spectrometry (ICP-MS) was employed to quantify micro-, trace-, ultra-trace, and toxic elements. The study showed the occurrence of elements at the ppm (Na, K, P, Ca, Mg), ppb (Al, Rb, Zn, Fe, Sr, Cu), and ppt (Ce, La, Nd, Tb) levels. Hierarchical clustering using Ward’s method and k-means analysis revealed four distinct clusters, including two major clusters representing the baseline macro- and microelement profile characteristic for the studied population. Correlations between elements revealed statistically significant (p < 0.05) positive and negative correlations between elements with (I) chemical similarity Ce-La, Cs-Rb, Rb-K, Ca-P, Zn-Cu, and Cs-K; (II) a possible common environmental origin, Cd-P, and Rb-P; (III) involvement in similar biological processes as K-P; and (iv) a common geochemical origin and similar biological functions, i.e., Se-Zn. The study identified several quantitative trends in the demographic and medical characteristics of the participants. Alcohol users had significantly higher Zn concentrations than non-alcohol users; women had significantly higher Ca concentrations than men; higher BMI correlated positively with Cs and negatively with Be and Cr levels; and Cu, Sb, Cd, Se, and Ca concentrations increased with age. The presence of several toxic and potentially toxic elements was identified in the vitreous body: Al (>10 ppb); Cd, Cr, Pb, Ni, Mn; and Ba (<10 ppb); As, Hg, Sb, Tl, Bi, Be (<1 ppb). The study showed that, within a given geographic region, the accumulation profiles of toxic metals are quite homogeneous, indicating common sources of exposure. Full article

The development of functional cereal-based products with enhanced nutritional and biological value is a priority in modern food science, as single-grain cereals often exhibit limitations in amino acid balance and micronutrient composition. This study aimed to develop, optimize, and evaluate a multicomponent cereal-based food mixture with improved functional and nutritional properties. A mixture composed of rice, buckwheat, oats, and corn was formulated using computer-based modeling to optimize essential amino acid composition and energy value in accordance with WHO recommendations. The technological process included cleaning, sorting, hydrothermal treatment, dehulling, drying, cooling, grinding, and subsequent blending of the cereal components. Standard methods were used to assess organoleptic properties, chemical composition, mineral and vitamin content, microbiological safety, and shelf life at a moisture content of 13–14%. The developed mixture demonstrated favorable sensory characteristics and a balanced chemical composition, with a protein content of 14.43 g/100 g, carbohydrates of 59.92 g/100 g, fat of 4.48 g/100 g, and an energy value of 322.74 kcal (1351 kJ) per 100 g. The product was rich in essential macro- and microelements and B-complex vitamins, met all microbiological safety requirements, and exhibited a shelf life of 4–5 months. These findings indicate that the proposed multicomponent cereal mixture is nutritionally balanced, safe, and suitable for functional and health-oriented food applications. Full article

The growing interest in functional beer production has led to the exploration of unconventional raw materials, such as hemp (Cannabis sativa L.), for brewing applications. This study aimed to evaluate the volatile organic compound (VOC) profile and the macro- and microelement composition of barley wort enriched with varying proportions (10% and 30%) of malted and unmalted hemp seeds, using solid-phase microextraction followed by gas chromatography–mass spectrometry (SPME–GC–MS) and atomic absorption spectrometry (AAS). A total of 64 VOCs were identified across four wort variants: control (barley malt only), 10% malted hemp, 30% malted hemp, and 30% unmalted hemp. The aroma profile was significantly influenced by compounds such as 2,3-butanediol, 1-hexanol, 3-methyl-1-butanol, 3-hydroxy-2-butanone, hexanoic acid, and 4-vinylguaiacol (p < 0.001). Principal component analysis (PCA) revealed clear separation between wort types based on the relative abundance of alcohols, acids, ketones, and phenols, indicating a progressive shift from sweet/malty toward acidic, green, and herbal aroma notes as hemp addition increased. Notably, unmalted hemp seeds resulted in a pronounced dominance of hexanoic acid, which may contribute to earthy and rancid sensory attributes. The evaluation of selected mineral elements showed that the key macroelements differentiating the worts were potassium, magnesium, phosphorus, and calcium, while among the microelements the distinguishing elements were manganese, iron, and sodium. These findings demonstrate the strong modulating effect of aromatic hemp-derived materials on the aroma composition and selected mineral content of brewing worts, supporting their targeted use in novel beer formulations. Full article

Amidst rapid urbanization and modernization, numerous traditional villages in China face severe challenges, including landscape homogenization and the erosion of their distinctive characteristics. Addressing this issue requires a method capable of systematically identifying, analyzing, and reconstructing both the landscape and its underlying cultural features. This study proposes a digital analytical approach that integrates multimodal artificial intelligence with landscape language theory to address the homogenization of cultural landscapes in traditional Chinese villages. Taking Xinye Village in Zhejiang Province as a case study, the research systematically decodes its landscape spatial narratives and underlying cultural genes. This framework systematically deconstructs village landscapes across four levels: “vocabulary, context, grammar, and semantics”. The village image database is first automatically recognized and statistically analyzed by computer vision technology, which extracts 31 core landscape vocabulary items from three main categories and nine subcategories. Second, Retrieval-augmented Generation technology is employed to synthesize from the constructed domain-specific corpus, a natural context structured around Yuhua Mountain and Daofeng Mountain, as well as a cultural context based on ancestral hall order, connected through folk activities, and idealized by farming and reading passed down through generations. Building on this framework, a multimodal model was used to examine the spatial composition and combinatorial laws of landscape features. Six essential dimensions—spatial layout, visual order, element combination, functional relationships, circulation layout, and scale correlations—revealed the spatial grammar of shuikou landscape. Lastly, the semantic values conveyed by the landscape vocabulary were thoroughly analyzed across three dimensions—form, function, and culture—by integrating a knowledge base. This work creates a landscape language atlas of Xinye Village by combining these studies and using a linguistic model of “character-word-sentence-paragraph”. By methodically deciphering the clan’s cultural code of “farming and reading passed down through generations”, this clearly reconstructs the spatial narrative logic from micro-elements to macro-patterns. This research not only advances the study of landscape language in traditional villages from qualitative description toward a systematic, digital, and interpretable paradigm but also provides an operational theoretical and methodological foundation for the in-depth interpretation, conservation, and transmission of traditional village cultural landscapes. Full article

The negative effects of soil heavy metal contamination on food production could be mitigated using nature-based solutions, i.e., plant-growth-promoting rhizobacteria (PGPR). Yield of Lactuca sativa L. has been shown to increase by seed inoculation with biofilm of Bacillus subtilis subsp. spizizenii. The aim of this work was to assess whether this promoting effect occurs even in the presence of toxic concentrations of copper (Cu) and zinc (Zn). First, germination rates of lettuce seeds with increasing Cu and Zn concentrations were assessed. Then, lettuce plants growing from inoculated and non-inoculated seeds were cropped on substrates with excess Cu and Zn. Above- and below-ground lettuce variables were measured, and leaf macro- and microelements were determined. Germination was more severely affected by Cu overload than by Zn overload, while this trend was reversal for plant growth. Seed inoculation enhanced germination and increased plant growth assessed by root and shoot biomass, plant height and leaf area. For example, seed inoculation increased lettuce root and aerial biomass of lettuce growing on a metal- free substrate by 68% and 62%, respectively. This practice also promoted lettuce growth in metal-overloaded substrates, increasing aerial and root biomass by 32% and 29%, respectively, in connection with Cu, and by 260% and 183% when it came to Zn. Both Cu or Zn accumulated in the edible parts of lettuce growing on contaminated substrates, but seed inoculation did not mitigate metal uptake in any case. Except for Cu and Zn, macronutrients, micronutrients and heavy metal levels in lettuce leaves were affected neither by excess metal nor by seed inoculation. Altogether, B. subtilis biofilm has been proven to be an effective seed inoculant promoting seed germination and plant growth even in the presence of heavy metals. Adverse health effects due to metal accumulation in the lettuce edible parts are not expected to increase following seed inoculation. Full article

Although plastic film mulching enhances crop yield, it impedes water infiltration, potentially restricting agricultural productivity. To address this issue, we evaluated the effects of different mulching methods on cauliflower growth, yield performance, quality traits, soil properties, and irrigation water use efficiency. We implemented three mulching treatments and two control groups: combined straw and plastic film mulching (T1), partial straw mulching (T2), full straw mulching (T3), no mulching (CK1), and plastic film mulching alone (CK2). These treatments were applied to two consecutive crops of cauliflower over a two-year period (2019–2020) in the arid and semi-arid regions of Gansu Province, China. Our findings revealed that T1 significantly enhanced plant height, stem diameter, and both above- and belowground fresh biomass compared to CK2. Moreover, T1, T2, and T3 promoted the accumulation of nitrogen, phosphorus, and potassium in the roots, stems, and leaves, as well as the concentrations of macro- (N and K), meso- (Ca and Mg), and micro-elements (Fe, Mn, Cu, and Zn) in the cauliflower heads. Compared to CK2, the soluble sugar and vitamin C contents increased by 17.43% and 8.68% in T1, and the soluble protein contents increased by 13.10% and 9.50% in T2 and T3 compared to CK2. Conversely, the nitrate content decreased by 28.28%, 42.06%, and 31.54% in T1, T2, and T3, respectively. Additionally, T1 increased economic yield and irrigation water use efficiency by 16.36–23.80% and 23.94–36.88% in the two years, along with notable improvements in the soil’s total nitrogen, total phosphorus, available phosphorus, and organic matter content. Multivariate classification modeling using principal component analysis (PCA) and hierarchical cluster analysis (HCA) further indicated that T1 enhanced cauliflower quality, yield, and irrigation water use efficiency and boosted soil fertility. These findings provide valuable insights for sustainable agricultural practices in arid and semi-arid regions. Full article