Search Results (28)

In this paper, a series of polyaniline (PANI)/Ti₃C₂ MXene composites (PMCs) with a biomimetic structure were prepared and employed as an anticorrosion coating application. First, the PANI was synthesized by oxidative polymerization with ammonium persulfate as the oxidant. Then, 2D Ti₃C₂ MXene nanosheets were prepared by treating the Ti₃AlC₂ using the optimized minimally intensive layer delamination (MILD) method, followed by characterization via XRD and SEM. Subsequently, the PMC was prepared by the oxidative polymerization of aniline monomers in the presence of Ti₃C₂ MXene nanosheets, followed by characterization via FTIR, XRD, SEM, TEM, CV, and UV–Visible. Eventually, the PMC coatings with the artificial biomimetic surface structure of a macaw feather were prepared by the nano-casting technique. The corrosion resistance of the PMC coatings, evaluated via Tafel polarization and Nyquist impedance measurements, shows that increasing the MXene loading up to 5 wt % shifts the corrosion potential (E_corr) on steel from −588 mV to −356 mV vs. SCE, reduces the corrosion current density (I_corr) from 1.09 µA/cm² to 0.035 µA/cm², and raises the impedance modulus at 0.01 Hz from 67 kΩ to 3794 kΩ. When structured with the hierarchical feather topography, the PMC coating (Bio-PA-MX-5) further advances the E_corr to +103.6 mV, lowers the I_corr to 7.22 × 10⁻⁴ µA/cm², and boosts the impedance to 96,875 kΩ. Compared to neat coatings without biomimetic structuring, those with engineered biomimetic surfaces showed significantly improved corrosion protection performance. These enhancements arise from three synergistic mechanisms: (i) polyaniline’s redox catalysis accelerates the formation of a dense passive oxide layer; (ii) MXene nanosheets create a tortuous gas barrier that cuts the oxygen permeability from 11.3 Barrer to 0.9 Barrer; and (iii) the biomimetic surface traps air pockets, raising the water contact angle from 87° to 135°. This integrated approach delivers one of the highest combined corrosion potentials and impedance values reported for thin-film coatings, pointing to a general strategy for durable steel protection. Full article

Understanding how children and adolescents perceive zoo animal welfare provides insights into public views on captive conditions. This study used a mixed-methods approach to investigate these perceptions. A total of 254 participants (aged 7–18) were surveyed, with 113 completing the full circuit, which included species from distinct phylogenetic groups: monkey, macaw, caiman, frog, fish, and tarantula. Qualitative analysis (n = 254) evaluated children’s recognition of animal needs, whereas quantitative analysis (n = 113) examined how phylogenetic distance, participants’ emotional state, and overall welfare perception influenced animal and environmental assessments—a structured survey evaluating perceived animal health, enclosure conditions, and behavioral expression. Participants’ understanding of animal welfare extended beyond basic needs, recognizing the importance of species-specific behaviors and appropriate environments. Environmental and animal assessment scores were significantly influenced by species, participants’ emotional states, and overall welfare perceptions: fish received the highest scores, positive emotions were associated with higher ratings, and a better overall welfare perception correlated with more favorable assessments. We inferred that phylogenetic distance, emotional state, and general welfare impressions shaped how children and adolescents evaluate animal welfare. The study supported zoo-based educational strategies and reinforced the role of zoos in promoting welfare awareness and conservation-oriented attitudes. Full article

The scarlet macaw (Ara macao) is a charismatic species that is native to Central and South America and commonly housed in captivity. Gaps in knowledge about these birds’ behavioral ecology in the wild hinders both in situ and ex situ management and conservation efforts for this species. We conducted seventeen days of observations of free-living scarlet macaws in two locations in Costa Rica, in February 2022, with the aims of (1) advancing our knowledge of this species’ natural behavioral ecology, and (2) generating data for comparison with captive macaws to help to inform their ex situ management (e.g., enclosure design and enrichment). Routes were walked within two locations—Bosque Escondido (BE), release area for captive-bred reintroduced individuals and no extant wild population, and Punta Leona (PL), an area of natural habitat within a resort inhabited by wild scarlet macaws—and focal interval sampling of multiple scarlet macaws in a group was used to record behavior and space use. The macaws at both locations were generally active, spent most of their time high in the tree canopy, relied on climbing to move within it, and used a wide variety of supports. The macaws at PL spent significantly more time feeding and locomoting, and less time perching, than those at BE, possibly due to differences in resource availability, rearing conditions, and age. Furthermore, the wild scarlet macaws at PL exhibited a variety of foraging strategies to acquire and manipulate food items within the tree canopy, including frequent use of their feet during manipulation. Despite limitations caused by a small sample size, a short period of observations, and differences between the two populations observed, this study provides insights into the behavioral ecology of scarlet macaws in the wild, which can be used for behavioral assessments of captive macaws while informing their ex situ management, with applications to animal welfare and captive breeding programs. Full article

Avian brood reduction was initially thought to be driven by insufficient food supply. Now it is more commonly considered a consequence of asynchronous hatching and resulting siblicide, direct filial infanticide (where parents kill specific chicks) or indirect filial infanticide (where parents starve specific chicks). In psittacines, brood reduction has been reported, but the mechanisms and causes remain unexplored. In this paper, we test the hypotheses that Scarlet Macaw chick starvation is driven by (1) sibling aggression, (2) food scarcity, and (3) parental food allocation based on (a) chick hatch weight and (b) chick age differences. We documented wild Scarlet Macaw behavior in lowland Peru in 37 nests over 19 seasons using morphological measurements and nest videos. Chick starvation was the leading cause of chick mortality (27% of all second-hatched chicks starve, and nearly all third- and fourth-hatched chicks starve). We found no evidence that starvation was caused by (1) sibling conflicts or (2) food availability. We did find parental food distribution favors first-hatched chicks, with larger age differences increasing the chances of second chick starvation. This study offers insights into brood reduction among Neotropical cavity-nesting birds and enhances our capacity to develop scientifically informed management strategies to support endangered psittacines. Full article

As habitat loss and other threats accelerate, ecological restoration and reintroduction science are becoming progressively more important. The psittacines are among the most endangered bird groups and are prime candidates for restoration through reintroduction. Unfortunately, post-release survival of captive-raised animals is often quite low because, in part, of high predation rates, low site fidelity, poor flight ability, and low flock cohesion. Current best practices in parrot release hold the birds in captivity for a year or more and include distinct methods to address each of these challenges. Here, we conduct a small-scale, proof-of-concept study using free flight methods and human-socialized trained adult birds to hand raise and release a group of six fledgling Blue-and-yellow Macaws in their historical range in southeastern Brazil. All six released birds showed strong flock cohesion and fidelity to the release site, avoided predation, and survived without supplemental feeding for over one year. One bird was captured by local people but was recovered and rereleased and it has reintegrated into the group and is still alive and doing well. The human-socialized trained adult birds modeled both desirable behaviors (flocking, foraging, reacting to predators) and undesirable behaviors and they were returned to captivity before the conclusion of this study. Our study suggests that free flight training has great potential to help captive-raised young attain a broad array of vital skills needed for survival post-release. Full article

A 3-year-old male scarlet macaw parrot (Ara macao) was presented to the Exotic Animal Clinic at the Faculty of Veterinary Medicine, Iași University of Life Sciences (Iași, Romania) for its postmortem examination. According to the owner, the parrot had been raised only in captivity and after 5 days of inappetence, lethargy, and mild respiratory clinical signs, the parrot died. The post mortem examination revealed various-sized granulomas and caseous plaques in the lungs, air sacs, spleen, intestinal serosa, and liver. Microscopically, the granulomas were characterized by a necrotic center and the infiltration of numerous multinucleated giant cells and epithelioid-like cells and by the presence of hyphae typical of Aspergillus spp. Moreover, in the liver tissue, a diffuse inflammation, with numerous fungal hyphae, was noted. The fungal culture and the PCR assay allowed for the isolation and identification of Aspergillus fumigatus from the lung and liver samples. The macroscopical lesions and the histopathological findings, with the fungal isolation and molecular confirmation of Aspergillus fumigatus by nested PCR, provided the basis for the diagnosis of disseminated aspergillosis. To the authors’ best knowledge, this is the first report of disseminated infection caused by Aspergillus fumigatus in a scarlet macaw parrot (Ara macao). Full article

Chlamydiosis, caused by Chlamydia psittaci is a bacterial infection found in at least 465 species of birds worldwide. It is highly contagious among birds and can spread to humans. In birds, the disease can manifest itself in acute, subacute, and chronic forms with signs including anorexia, diarrhea, lethargy, weight loss, or, occasionally, mucopurulent or serous oculonasal discharge. This article describes an outbreak of chlamydiosis that occurred in a commercial psittacine breeding aviary in 2021 in Buenos Aires province, Argentina. In total, 16 juvenile blue-fronted parrots, more than 60 blue-fronted parrot chicks, and 2 adult macaws died during the outbreak. In all cases, clinical signs were weight loss, diarrhea, yellowish green excrement, and respiratory distress. The necropsy of four juvenile blue-fronted parrots, two blue-fronted parrot chicks, and two adult macaws revealed cachexia, hepatomegaly, splenomegaly, splenic petechial hemorrhages, ascites, pulmonary edema, and hydropericardium. Histologically, multifocal lymphoplasmacytic and heterophilic airsaculitis, multifocal lymphoplasmacytic and necrotizing hepatitis with intracytoplasmic elementary bodies, multifocal necro-heterophilic hepatitis, multifocal lymphoplasmacytic nephritis, and diffuse heterophilic pneumonia were found. A presumptive diagnosis was established based on gross and microscopic lesions, and it was confirmed using immunohistochemistry and polymerase chain reactions. The sequencing and phylogenetic analysis of the ompA gene revealed genotype A and B of Chlamydia psittaci. Full article

Birds kept in zoological institutions are highly exposed to gastrointestinal (GI) parasitism caused by coccidia and nematodes. The current research aimed to characterize the avian GI parasitic fauna in several zoological collections in Portugal and Spain. During the full year of 2022, a total of 120 fecal samples were collected from four zoological institutions: Lisbon Zoo, Olivais Pedagogical Farm, and Avian Biodiversity Center (Lisbon, Portugal), and Avifauna park (Lugo, Spain). Analysis was conducted in domestic bird species (autochthonous and exotic poultry breeds), and 18 different exotic bird species like Galliformes (peacock, pheasant), Anseriformes (duck), Psittaciformes (parrot, macaw, cockatiel, parakeet, cockatoo), Coraciiformes (motmot), Charadriiformes (avocet), Strigiformes (owl), Phoenicopteriformes (flamingo), Struthioniformes (ostrich), Rheiformes (rhea), and Casuariiformes (emu, cassowary). Feces were processed using Mini-FLOTAC (MF), to identify parasitic forms and quantify their shedding (oocysts or eggs per gram of feces). Moreover, 15 fecal samples from pheasants were also processed using the McMaster method (McM), to compare the parasite shedding and frequencies between techniques. MF implementation allowed identification of coccidia infections in all bird collections. Also, peacocks of the Lisbon Zoo tested positive for Trichostrongylus tenuis and Strongyloides pavonis, and the exotic birds from Avifauna park were also positive for several nematode species, with Ascaridia sp., Capillaria sp., Strongyloides sp., and Syngamus trachea eggs being detected in pheasants’ feces. Moreover, the analysis of pheasants’ feces with MF detected prevalences of 33% for coccidia oocysts, and 47% for Capillaria sp. and Ascaridia sp. eggs, while McM detected prevalences of 13%, 27%, and 40% for the respective parasite taxa, with no differences being observed between methods (p = 0.39, p = 0.45, and p = 0.50, respectively). This research provided more scientific support regarding the importance of using Mini-FLOTAC in routine parasitological diagnosis in birds kept at zoological institutions. Full article

Medio Period (AD 1200 to 1450) Casas Grandes shamans used tobacco and possibly other entheogens to initiate trance states that allowed their spirits to travel across the cosmos. These trance experiences involved a sense of vertigo and soul flight that is cross-culturally common and occurs with tobacco-based nicotine intoxication. The Medio Period shamans also relied on and interacted with other-than-human persons during their travels, including macaw- and serpent-linked deities, as well as animated objects designed to participate in their shamanic journeys. The animated objects included Playas Red and Chihuahuan Polychrome pottery effigies of humans, macaws, and snakes with shamanic themes that represented the spirit world. I propose these pots were animate “pot-people” created for shamanic rituals. They were created with unusual designs including painted images and incised patterns that emphasized the spinning/vertigo that was central to the shamans’ soul flight experience. In some cases, the pots were literally spun, as evidenced by the distinctive wear patterns on their bases. The shamanic designs on the pots that reflected the upper and lower worlds, the depiction of spinning in the pottery decorations, and the literal spinning of some pots reflected the sympathetic and mimetic magic that linked them to the spirit world. They were imbued with the liminal nature of the creatures they depicted, and the symbolic and occasional literal emphasis on spinning would allow them to enter into a shamanic trance in a manner similar to their human counterparts. They, thus, were designed to enter into ASC in a manner that paralleled their human counterparts. Full article

We describe efforts to reintroduce the extinct-in-the-wild Spix’s Macaw (Cyanopsitta spixii) within its historical range in eastern Brazil. Twenty captive-reared Spix’s Macaws were released, along with 15 Blue-Winged Macaws (Primolius maracana), as heterospecific flocks in two events during the dry and rainy seasons of 2022. We monitored the release groups through daily observation and telemetry tracking from early June 2022 to early June 2023. We documented an overall first-year survival of 58.3% (CI: 37.8–78.7%), with 65% of Spix’s Macaws establishing a stable area of activity within 5 km of the release site, excluding any temporary long-distance forays. Eighty-five percent of released Spix’s Macaws exhibited flock cohesion, including interactions with and integration into wild Blue-Winged Macaw groups at the release site. Several released Spix’s Macaws formed pair-bonds with conspecifics and engaged in nest cavity exploration, breeding behavior, and territorial defense of nest sites, with three females also laying and incubating eggs. One nesting pair successfully hatched and reared chicks in an artificial nest cavity. These releases employed a novel reintroduction strategy using a surrogate species model, the sympatric Blue-Winged Macaw, to “mentor” and facilitate post-release adaptation by the target species and increase the total number of individuals in the release cohort. Lastly, participatory monitoring by local citizens is considered a way to engage and involve local communities in species and habitat conservation and potentially create new employment opportunities in the region. Full article

Although the use of incision-free endoscopy for foreign body (FB) removal in dogs and cats has been extensively documented, its application in birds remains limited. Thus, we present the endoscopic removal of gastrointestinal (GI) FBs from psittacine birds, employing different patient positioning and anesthesia methods. Two blue-and-yellow macaws (Ara ararauna) and a Triton cockatoo (Cacatua galerita triton) were examined. X-ray imaging revealed FBs situated in the proventriculus in each case. The FBs, all identified as feeding tubes, were safely removed using grasping forceps during the endoscopic procedure, and no severe complications occurred. Based on the outcomes of each operation, the most suitable patient position may be ventral recumbency rather than dorsal recumbency, with the use of a mask or endotracheal intubation, depending on the anticipated operation time. However, a larger number of cases would be necessary to confirm the optimal patient positioning and anesthesia method. Full article

The Military Macaw (Ara militaris) is an endangered bird species with disjunct geographic distribution across the Neotropics, consisting of three recognized subspecies: One in Mexico (A. m. mexicanus) and two in South America (A. m. militaris and A. m. bolivianus). However, due to the limited phenotypic differentiation between these allopatric taxa, their taxonomic status has been the subject of debate. In this study, we explored mitochondrial DNA (mtDNA) variability to determine the phylogeographical pattern through phylogenetic and ecological modelling analyses. We also aimed to describe the evolutionary relationships of twelve A. militaris populations. We identified 41 haplotypes in the 300 bp region of the Cytochrome b (Cyt-b) gene of the mtDNA and low nucleotide diversity. The observed phylogeographic structure suggests the existence of two clades: One composed of A. m. militaris and A. m. bolivianus and another consisting solely of A. m. mexicanus. The A. m. mexicanus clade further divides into two recognized subclades: Sierra Madre Oriental and northeastern portion of the Sierra Madre Occidental. Ecological analyses revealed that the niche similarity between these lineages was lower than expected by chance. Additionally, results from low cross-prediction tests indicated that the two lineages have inhabited different environmental spaces since the Late Pleistocene. This divergence may be associated with a steep ecological gradient and contemporary geographical barrier. Based on our results, we suggest that at least the A. m. mexicanus has a divergent evolutionary history; therefore, it should be considered as a different evolutionarily significant and management unit. We recommend that future conservation strategies in Mexico incorporate effective protection measures, including habitat preservation and the reduction of illegal trade, to ensure the preservation of viable populations. Full article

Visitors are a prominent feature of the zoo environment and lives of zoo animals. The COVID-19 pandemic led to repeated and extended closure periods for zoos worldwide. This unique period in zoological history enabled the opportunity to investigate the consistency of behavioural responses of zoo animals to closures and subsequent reopenings. Bennett’s wallabies (Notamacropus rufogriseus), meerkats (Suricata suricatta), macaws (red and green: Ara chloropterus; blue and yellow: Ara ararauna; military: Ara militaris) and rabbits (Oryctolagus cuniculus domesticus) held at four zoological collections in the United Kingdom were studied during COVID-19 closures and subsequent reopening periods. Facilities were closed for three time periods during 2020 and 2021: March–June/July 2020; November–December 2020; January–April/May 2021. Behavioural data were captured during closures (maximum n = 3) and reopening periods (maximum n = 3) during five-min scans using instantaneous scan sampling with a one-minute inter-scan interval. General linear models (GLMs) and general linear mixed models (GLMMs) were used to investigate the relationship between observed behaviours and open/closed periods. Changes were observed in behaviour between open and closure periods in all species, and in some instances changes were also observed over time, with animals responding differently to different closure and reopening periods. However, no overt positive or negative impacts of the closures or reopening periods were identified for these species. The study species may have different relationships with zoo visitors, but no clear differences were seen across the species studied. The unique opportunity to study animals over a long period of time during repeated closure periods enabled a greater understanding of the impact of zoo visitors on animals. As with other work in this sphere, these data support the adaptability of zoo animals to zoo visitors. This work contributes to the growing field of research undertaken during the COVID-19 periods and enhances our understanding of the impact that these zoological closures had on a wider body of species in a number of facilities. Full article

The diversification of biomass resources is key to the transition towards a bioeconomy. Acrocomia spp., a neotropical genus of palms, is an example of plants’ diversity potential for a sustainable bioeconomy. Acrocomia’s adaptability to environments outside rainforests, its specific fruit properties and high yields has generated the interest of researchers and entrepreneurs, triggering its introduction as a multipurpose oil crop. Developing sustainability-oriented and knowledge-based acrocomia value webs requires a collaborative, systems approach from the outset. Fostering an inter- and transdisciplinary dialogue on acrocomia through a participatory workshop with both academic and non-academic actors contributed to this endeavor. This allowed the identification of priorities, knowledge gaps, and stakeholder roles, and served as the basis for the co-creation of a research and development roadmap. Key steps for the introduction of acrocomia include intertwined technical aspects relating to the development of planting material, cultivation systems, processing technologies and applications, market entry, and value web governance aspects. A broad collaboration among scientists, the public and private sectors, farmers, and civil society, is required for the development of acrocomia value webs. The incorporation of sustainability and a consideration of context in the design and development phases are fundamental to fostering the sustainable performance of acrocomia value webs. Full article

Several fungi species are reported to act as opportunistic agents of infection in avian species. After the isolation of Exophiala spp., a dematiaceous fungal pathogen associated with a mucosal lesion in a military macaw (Ara militar), samples were collected from another 24 birds of the order Psittaciformes to study the possibility of Exophiala spp. being part of the commensal microbiota of these animals or its possible association with other clinical conditions. Swab samples were collected from the trachea and/or choanae of the birds and inoculated in Sabouraud chloramphenicol agar for fungal isolation. After incubation, fungal species were identified through their macroscopic and microscopic morphology. The presence of Exophiala spp. was identified in 15 of the 25 birds sampled and no statistical association was found between the clinical record of the birds and the fungal isolation. Our results suggest that Exophiala spp. can colonize the upper respiratory airways of psittaciform birds and has a low pathogenic potential in these animals. To the authors’ knowledge, this is the first report of Exophiala spp. isolation from samples of the upper respiratory tract of Psittaciformes. Full article