Search Results (12,969)

Cherry tomato is a notable dietary source of metabolites associated with antioxidant functions. However, how ripening reshapes primary, specialized, and volatile metabolites remains incompletely resolved. Green-ripe and red-ripe fruits were comparatively analyzed using targeted HPLC assays for quality indices and vitamins, UPLC–MS/MS for non-volatile metabolites, and HS-SPME–GC–MS for volatiles. Ripening was accompanied by a pronounced accumulation of lycopene and an increase in soluble solids, reflecting a shift of sugars toward glucose and fructose while sucrose remained low. Organic acids declined overall, with citric acid remaining predominant. The free-amino-acid pool expanded, with redistribution from GABA toward glutamate and aspartate. Vitamins exhibited stage-dependent patterns; antioxidant-related vitamins (A, E, and C) were higher at the red-ripe stage, indicating a compositional enhancement relevant to nutritional quality. Non-volatile metabolomics revealed 618 differentially accumulated metabolites, with phenolic acids, flavonoids, alkaloids, amino acids, and lipids as major classes. Phenolic acids and flavonols, dominated by hydroxycinnamoyl-quinic acids and quercetin/kaempferol glycosides, accumulated at the red-ripe stage, whereas steroidal glycoalkaloids decreased, suggesting conversion away from bitter or anti-nutritional constituents. GC–MS profiling identified 788 volatiles, with esters, terpenoids, and ketones contributing more than half of the volatilome. Ripening favored fruity–floral odorants such as β-ionone and (5Z)-octa-1,5-dien-3-one, while reducing green-leaf aldehydes. These stage-specific shifts in metabolite composition jointly define the sensory and nutritional maturation of cherry tomato. The identified metabolite markers provide a foundation for evaluating fruit maturity and guiding breeding toward improved quality attributes. Full article

Recreation, aesthetic appreciation, identity, and spiritual values are among the cultural ecosystem services (CES) produced by long-distance historic and pilgrimage trails. However, it is still difficult to convert these experiential benefits into quantifiable economic flows. This study collected 560 valid responses from an in-field survey conducted along the Via degli Dei (Bologna–Florence). Robust visitor clusters were created using Gower dissimilarities, Partitioning Around Medoids (PAM), silhouette diagnostics, and Factor Analysis for Mixed Data (FAMD). Each cluster was then profiled according to seasonal patterns, information channels, individual-level, per-category expenditures (accommodation, food, transport, services, and equipment), as well as motivations. Four segments are identified—Student Campers (low-budget, peak-summer), Working-Age Male B&B Hikers (short stays, B&B), Young Women on Mixed Lodging (mixed accommodation), and Midlife Comfort-Seekers (higher spend, shoulder-season)—underpinning our spending, seasonality, and managerial implications. Student Campers had the lowest absolute expenditures, while Midlife Comfort-Seekers had the highest (median lodging €180; food €175). The study offers practical levers for route governance (targeted communications, low-impact lodging strategies, shoulder-season promotion) to improve local value capture while reducing environmental pressure by connecting typologies to monetary CES flows. The findings provide a reproducible model for implementing recreational CES on historical-cultural tours. Full article

Cyber-sexual crimes have become a growing concern in the digital age, as rapid technological progress continues to create new forms of violence and victimization. These offenses affect society unevenly, striking more intensely among minors, women, and other vulnerable groups. Their prevalence is shaped by structural inequalities, educational, economic, and technological, that condition both exposure to digital risks and the capacity for protection. Although international research has connected these disparities with digital victimization, evidence from Spain remains limited. The aim was to analyze the regional distribution of cyber-sexual crimes in Spain between 2011 and 2022 and to explore how education, income, and digital access relate to their incidence. To this end, official data from the Spanish Statistical Crime Portal (PEC) were combined with structural indicators provided by the Spanish National Institute of Statistics. The analysis encompassed reported cases of sexual abuse, sexual harassment, corruption of minors, online grooming, exhibitionism, pornography, and sexual provocation, using standardized incidence rates per 100,000 inhabitants. Statistical methods included ANOVA with post hoc comparisons, correlation analyses, and K-means clustering to identify territorial patterns. Results revealed a sustained national increase in cyber-sexual crimes, with grooming and sexual harassment showing the most pronounced growth. The Balearic Islands (mean 4.9), Canary Islands (4.0), and Andalusia (3.9) registered the highest incidence rates, well above the national average (3.0). Educational disadvantages and low income were linked to sexual abuse and corruption of minors, whereas greater digital connectivity, expressed through higher mobile phone use, broadband access, and computer ownership, was strongly associated with grooming and other technology-facilitated offenses. Cluster analysis identified three distinct territorial profiles: high-incidence regions (Balearic and Canary Islands, Andalusia), intermediate (Murcia, Madrid, Navarre, Valencian Community), and low-incidence (Galicia, Catalonia, Castile and León, among others). In conclusion, the findings demonstrate that cyber-sexual crimes in Spain are unevenly distributed and closely linked to persistent structural vulnerabilities that shape digital exposure. These results underscore the need for territorially sensitive prevention strategies that reduce educational and economic inequalities, foster sexual and digital literacy, and promote safer online environments. Without addressing these underlying structural dimensions, public policies risk overlooking the conditions that sustain regional disparities and limit adequate protection against technology-driven sexual crimes. Full article

Industrial hemp phytochemistry is shaped by genetics and agronomic management, yet field studies integrating both remain scarce. The combined effects of cultivar, planting density, fertilization, and flowering time on cannabinoids, terpenes, and antioxidants in Cannabis sativa L. were evaluated. A field trial was conducted in Mallorca (2023) using two cultivars (Enectaliana, Enectarol) grown at two densities (Sector 1 ≈ 2.3 plants m⁻²; Sector 2 ≈ 4.6 plants m⁻²), with sampling from flowering onset (week 0) to week 5. In Enectaliana, fertilization (with vs. without) was tested. Enectaliana displayed CBD/CBDVA-dominated profiles, whereas Enectarol was CBG-predominant; THC remained consistently low. Effects were assessed via three-way ANOVA (Density × Time × Cultivar; Density × Time × Fertilization). The cultivar and time explained most of the variance, with interactions modulating magnitudes without altering effect hierarchies. Planting density acted as a second-order modulator, modulating concentrations without reversing cultivar rankings. Terpenes peaked early and generally declined as flowering progressed, with cultivar-dependent trajectories. Total phenolics and antioxidant activity (ABTS, FRAP assays) increased steadily until week 5, with density and treatment effects. In Enectaliana, fertilization effects were selective: ABTS values tended to be higher in unfertilized plants at the end of the cycle, FRAP results showed a density interaction, and cannabinoids exhibited non-linear responses to nutrient supply. Full article

A Global Navigation Satellite System (GNSS) and Long Range (LoRa) technology play a crucial role in connected vehicles. The demand for antennas that cover both LoRa and GNSS bands is increasing. This work has developed a novel dual-band coplanar waveguide (CPW)-fed interleaved meander line antenna, incorporating a radiating element, ground plane, and feed. The antenna dimension is 90 × 90 × 1.635 mm³. The design employs a planar meander line configuration to effectively cover the 868 MHz LoRa and 1248 MHz GNSS bands. The antenna was integrated with a Frequency Selective Structure (FSS) to improve the parameters. The designed antenna provides sufficient bandwidth of 40 and 110 MHz for the LoRa and GNSS frequency bands, respectively. The CPW-interleaved meander line antenna attains a gain of −0.12 dBi at LoRa and 3.5 dBi at GNSS frequency. It achieves a voltage standing wave ratio of <2 and impedance of 50 Ω. The novelty of the proposed work is integrating FSS with a CPW-interleaved meander line antenna, which achieves dual-band operation. This dual-band low-profile configuration is suitable for connected vehicle communication. Full article

In this study, sheets of experimental high-carbon low-density steels (LDSs) with a thickness of 1.7 mm were processed in a combined tool designed for press-hardening. Press hardening, also known as hot stamping or hot press forming, is a manufacturing process used to create car body parts with exceptional mechanical properties and safety standards. These components often require tailored properties, meaning different mechanical characteristics in various parts of the component. LDSs have a lower specific density than conventional steels, so their use would be particularly suitable in automotive applications. Combined tools achieve distinct mechanical properties within a single part through thermomechanical processing. Simultaneous forming and heat treatment create tailored zones of high strength and ductility within the sheet metal. The hardened zone provides crashworthiness, while the more ductile zone absorbs kinetic energy and converts it into deformation energy. Hot stamping enables forming complex geometries from high-strength sheets with limited cold formability, a capability that can also be exploited for the aluminium-alloyed LDS under investigation in this work. Three different high-carbon LDSs with differences in chemical composition were subjected to this experiment, and the hardness, microstructure, and mechanical properties of the two areas of each sheet were evaluated. The aim is to determine their suitability for processing by press hardening and to try to achieve tailored properties (i.e., differences in ductility and strength across one part) as in a typical representative of 22MnB5 boron steel, where a strength limit of 1500 MPa at 5% ductility is achieved in the cooled part and 600 MPa at 15% in the heated part. Tailored properties were also achieved in the investigated LDS, but with only relatively small differences between the two tool areas. The omega profiles were produced by press hardening without visible defects, and it was possible to process the steels without any difficulties. Full article

This study investigated the combined effects of lactic acid bacteria (LAB) fermentation and different milling methods (wet, semi-dry, and dry) on the physicochemical properties of glutinous rice flour (GRF) and the texture of the final product. A systematic analysis of rice samples treated with three LAB strains (Lactiplantibacillus plantarum CGMCC 1.12974, Limosilactobacillus fermentum CICC 22704, and Lactobacillus acidophilus CICC 22162) revealed that fermentation pretreatment created favorable conditions for subsequent physical milling by degrading the protein network and modifying the starch structure. The results demonstrated that fermentation combined with dry or semi-dry milling significantly improved the whiteness of GRF and the contents of γ-aminobutyric acid (GABA), total phenols, and total flavonoids, while reducing the contents of damaged starch (except in samples fermented with Lb. acidophilus) and protein by 2.91–12.43% and 17.80–32.09%, respectively. The functional properties of the GRF were also optimized: fermented flour exhibited higher peak viscosity, lower gelatinization temperature, and higher gelatinization enthalpy. Texture profile analysis revealed that glutinous dumplings prepared from fermented dry/semi-dry milled GRF, particularly those fermented with Lp. plantarum, showed significantly reduced hardness and chewiness, along with significantly improved cohesiveness and resilience. Consequently, their texture approximated that of high-standard wet-milled products. Correlation analysis based on the top ten discriminative features selected by random forest identified peak viscosity and breakdown viscosity as the most important positive factors associated with superior texture (high resilience, high cohesiveness, and low hardness), whereas damaged starch content and protein content were key negative correlates. In summary, this study confirms that the combination of fermentation and milling exerts a beneficial influence on the functional quality of GRF. Full article

Concurrent consumption of ethanol (EtOH) and herbal preparations containing Withania somnifera (WS, ashwagandha) is increasingly common, but the neurobehavioral and molecular consequences of such interactions remain poorly characterized. This study investigated how three purified withanolides—withanolide A (WITA), withanone (WIN), and withaferin A (WTFA)—modulate the effects of acute EtOH exposure in zebrafish (Danio rerio) larvae. Locomotor behavior was quantified under EtOH concentrations ranging from 0 to 4.0%, and the expression of four GABAA receptor subunit genes (gabra1, gabra2, gabrd, gabrg2) was analyzed by qPCR. EtOH alone induced a biphasic locomotor response, with stimulation at low-to-moderate doses and suppression at higher doses. WITA and WIN modulated this pattern in a dose-dependent manner, preserving or enhancing hyperactivity, while WTFA consistently potentiated locomotor suppression. mRNA profile analysis revealed subunit-specific changes, including downregulation of gabra1 and gabra2, compound-dependent regulation of gabrd, and complex gabrg2 responses. These results demonstrate that individual withanolides distinctly shape behavioral and molecular outcomes of EtOH exposure, suggesting specific interactions at the level of inhibitory neurotransmission. The findings provide mechanistic insight into the combined effects of WS-derived compounds and EtOH and highlight the importance of considering such interactions in both experimental and applied contexts. Full article

Industrial hemp (Cannabis sativa L.; Cannabaceae), traditionally cultivated for fiber, also represents a valuable source of nutrient-rich seed oil. In this study, cold-pressed hemp seed oils from three Romanian varieties (Teodora, Silvana, and Armanca) were evaluated for their fatty acid composition, minor bioactive constituents, antioxidant activity, growth-promoting property toward probiotic strains in vitro, and molecular docking interactions with probiotic targets. Gas chromatography revealed a fatty acid profile dominated by linoleic (49.4–51.9%), oleic (16.3–22.8%), and α-linolenic acids (9.8–14.4%), resulting in favorable PUFA/SFA ratios (5.17–6.39) and ω-6/ω-3 ratios (3.93–5.53). The oils also contained phenolics (118–160 mg GAE/kg), chlorophylls (6.18–8.31 mg/kg), and carotenoids (2.58–3.37 mg/kg), which contributed to their antioxidant activity (DPPH inhibition 35.92 µM TE/100 g–43.37 µM TE/100 g). Broth microdilution assays against Lacticaseibacillus rhamnosus GG, L. paracasei ATCC BAA-52, and L. acidophilus ATCC 4356 demonstrated strain- and dose-dependent potential to promote probiotic growth under in vitro conditions. While L. rhamnosus and L. paracasei were inhibited at low concentrations and only mildly stimulated at higher levels, L. acidophilus showed robust growth promotion, reaching +54.7% effect and CP = 1.55 with Teodora oil at 16 mg/mL. Molecular docking highlighted strong binding affinities of γ-linolenic and linoleic acids with key metabolic enzymes involved in probiotic metabolism (hydratase, enolase, glyceraldehyde-3-phosphate dehydrogenase, ribonucleoside hydrolase), forming stable hydrophilic and hydrophobic interactions which are explored in defining the stability of the ligand-protein complexes. These results indicate that both major fatty acids and minor bioactive constituents contribute to the nutritional and antioxidant value of Romanian hemp seed oils and reveal a potential to promote probiotic growth under in vitro conditions, as supported by complementary in silico evidence. Full article

Objective: This study aimed to identify psychological characteristics associated with suicidal ideation among Palestinian university students in the West Bank during a period of escalating regional violence (October 2023), with data collected prior to the end of the war, a period marked by intensified political violence and collective trauma. The goal was to identify empirically derived psychological profiles of distress and coping using Latent Profile Analysis. Method: A cross-sectional survey of 900 students assessed depression, anxiety, self-efficacy, resilience, help-seeking attitudes, and suicidal ideation during the past 12 months. Latent Profile Analysis (LPA), logistic regression, and moderated mediation analysis were employed to investigate the relationships between distress, self-efficacy, resilience, and suicidal ideation. Results: Results indicate that depression and anxiety are associated with increased 12-month suicidal ideation, but greater self-efficacy appears to reduce this risk. The mediation analysis revealed that self-efficacy partially explains the relationship between distress and suicidal ideation; however, resilience did not have a significant moderating effect. The LPA identified three distinct psychological profiles, with the highest-risk group exhibiting significant distress and low self-efficacy. Conclusions: These results highlight the significant mental health burden faced by Palestinian youth and underscore the importance of internal psychological resources, particularly self-efficacy, that are associated with lower levels of suicidal ideation. Enhancing self-efficacy may offer a culturally relevant approach for prevention efforts in politically unstable environments. Full article

The growing demand for sustainable alternatives to animal and synthetic leathers has accelerated interest in mycelium-based materials as an eco-friendly solution for the fashion industry. This study explores the potential of mushroom mycelium to create leather-like materials that align with circular fashion principles. Five species of edible and medicinal mushrooms were cultivated on sawdust substrates and evaluated for their growth performance, physical properties, and suitability as leather substitutes. Growth analysis revealed distinct species-specific behaviors: Cubamyces flavidus and Lentinus squarrosulus exhibited rapid colonization, achieving full substrate coverage within five days and forming dense mycelial networks at 14 days. In contrast, despite growing more slowly, Sanghuangporus vaninii and Ganoderma gibbosum formed thicker, more compact mats that might be suitable for strong leather-like materials. Visual and structural assessments showed diverse textures, colors, and hyphal architectures resembling natural leather. Physical characterization revealed shrinkage ranging from 13.17% to 24.09%, higher than for cow tanned leather (>5%) and PU microfiber (0.1–1.2%), suggesting a need for stabilization treatments. Apparent densities ranged from 0.13 g/cm³ to 0.30 g/cm³, lower than those of cow leather (0.49 g/cm³) and PU leather (0.38 g/cm³), highlighting species-specific hyphal structures that influence flexibility, porosity, and strength. SEM imaging confirmed the presence of interwoven hyphal mats resembling the fibrous architecture of natural leather, with S. vaninii showing the most uniform and continuous structure. Water absorption was significantly higher in mycelium sheets, consistent with their microporous nature, though S. vaninii showed the lowest uptake, reflecting possible natural water absorption. Thermogravimetric analysis revealed three-stage degradation profiles, with S. vaninii and G. gibbosum retaining >35% mass at 400 °C, indicating strong thermal stability for processing techniques such as hot pressing and finishing. Overall, the results demonstrate mycelium-based leathers as a biodegradable, low-impact alternative that can replicate the visual and functional characteristics of traditional leather, with opportunities for further improvement in substrate optimization, eco-tanning, surface coating, and scalable production toward a sustainable fashion future. Full article

The bio-conversion of agricultural biomass into value-added products via solid-state fermentation (SSF) represents a cost-effective and eco-friendly approach, though it is often limited by low efficiency and prolonged processing times. While low-intensity magnetic fields (LMFs) have shown potential to enhance microbial metabolism and improve mass and heat transfer during SSF, the effects of conventional inhomogeneous magnetic fields remain inconsistent and may even cause localized microbial damage due to uneven field distribution. In this study, we designed and optimized a Helmholtz coil system capable of generating a highly homogeneous low-intensity magnetic field to overcome this limitation. Through electromagnetic simulation and experimental validation, an optimized aluminum profile-supported coil configuration was developed, achieving an average magnetic field intensity of 142.77 G under 70% power load with high spatial homogeneity (maximum deviation: ±1.32%). Applied to the solid-state fermentation of peanut meal, the homogeneous LMF treatment (40 G, 4 h) significantly increased peptide content by 77.76% compared to non-treated samples, and by 42.95% over traditional inhomogeneous LMF treatment. This work establishes homogeneous magnetic-field-assisted SSF as a novel, efficient, and scalable bioprocessing strategy, providing both a robust technological framework and new insights into the role of field uniformity in the magneto-fermentation of agricultural biomass. Full article

Extrachromosomal circular DNA (eccDNA) has been recognized as a key player in tumorigenesis and progression. However, eccDNA transcriptional regulatory mechanisms under DNA damage in cancer remain poorly characterized. Here, we used doxorubicin to induce DNA damage in the hepatocellular carcinoma cell line HepG2 and performed Circle-seq to profile eccDNAs before and after the damage. We observed a significant increase in the number, length, and chromosomal distribution density of eccDNAs following DNA damage. RNA-seq revealed that the expression of genes carried on eccDNA was positively correlated with eccDNA copy number under DNA damage. Further ATAC-seq profiling identified distinct chromatin characteristics at eccDNA breakpoint regions compared to other regions of eccDNA and linear genomic regions. Additionally, eccDNAs generated under DNA damage preferentially originated from linear genomic regions characterized by low GC content and hypomethylation. Finally, by integrating Hi-C and H3K27ac ChIP-seq, we uncovered that eccDNAs with mobile enhancer activity (ME-eccDNAs) display significantly enhanced chromatin interactions and H3K27ac enrichment after DNA damage. Overall, our findings systematically elucidate the DNA damage-driven mechanisms underlying eccDNA biogenesis, chromatin characteristics and transcriptional regulation in HCC HepG2 cells. Full article

Panax notoginseng is a valuable medicinal herb, but its sustainable production is constrained by long cultivation cycles and continuous cropping obstacles. Adventitious root culture presents a viable alternative. This study establishes a robust and sustainable platform for the efficient production of ginsenosides in P. notoginseng adventitious root cultures. We first systematically optimized the culture system, identifying leaf segments as the optimal explant due to their high callus induction rate (95.77%), low contamination, and renewable nature. Combined with optimized bioprocess parameters (3 g/L inoculation density, 50 g/L sucrose), this strategy addressed key practical bottlenecks. Beyond methodological advancement, our research provides novel mechanistic insights into the action of methyl jasmonate (MeJA). A key finding is the discovery that MeJA functions as a ‘precision metabolic switch,’ differentially regulating a critical branch point in the saponin pathway. It coordinately upregulates the protopanaxadiol (PPD)-type gene CYP716A47 while downregulating the protopanaxatriol (PPT)-type gene CYP716A53v2 that genetically explains the directed enrichment of specific ginsenosides. This integrated approach not only advances the fundamental understanding of elicitor action but also provides a scalable and controllable system for the industrial production of P. notoginseng phytopharmaceuticals with tailored saponin profiles. Full article

Background: High-throughput metabolomics studies have promoted the discovery of candidate biomarkers linked to atherosclerosis (AS). This narrative systematic review summarises metabolomics studies conducted in (1) individuals with subclinical AS (assessed by imaging techniques such as carotid intimal media thickness, IMT, and coronary artery calcium, CAC), (2) patients with established atherosclerotic plaques, and (3) individuals with AS risk factors. Methods: The systematic search was conducted in the PubMed database according to the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines. The inclusion criteria were as follows: (i) publication date between 2009 and 2024; (ii) identification of potential biomarkers for AS in subjects with a diagnosis of AS or with one or more traits characteristic of the disease (i.e., CAC or IMT); (iii) identification of potential AS biomarkers in subjects with atherogenic clinical conditions (i.e., Down’s syndrome, DS, polycystic ovarian syndrome, PCOS, and systemic lupus erythematosus, SLE); (iv) metabolomic studies; and (iv) studies in human samples. Exclusion criteria comprised the following: (i) studies on lipid metabolic diseases unrelated to AS, (ii) “omics” results not derived from metabolomics, (iii) reviews and studies in animal models or cell cultures, and (iv) systematic reviews and meta-analyses. Of 90 eligible studies screened, 24 met the inclusion criteria. Results: Across subclinical and overt AS, consistent disturbances were observed in amino acid, lipid, and carbohydrate metabolism. Altered profiles included branched-chain amino acids (BCAAs), aromatic amino acids (AACs) and derivatives (e.g., kynurenine–tryptophan pathway), bile acids (BAs), androgenic steroids, short-chain fatty acids (FAs)/ketone intermediates (e.g., acetate, 3-hydroxybutyrate, 3-HB), and Krebs cycle intermediates (e.g., citrate). Several metabolites (e.g., glutamine, lactate, 3-HB, phosphatidylcholines, PCs/lysophosphatidylcholines, lyso-PCs) showed reproducible associations with vascular phenotypes (IMT/CAC) and/or clinical AS. Conclusions: The identification of low-weight metabolites altered in both subclinical and overt AS suggests their potential as candidate biomarkers for early AS diagnosis. Given the steady increase in deaths from cardiovascular disease, a manifestation of advanced AS, this finding could have significant clinical relevance. Full article