Search Results (18,956)

Microbial fermentation is a key biotechnological tool for producing bioactive metabolites such as alkaloids, carotenoids, essential oils, and phenolic compounds, among others, with applications in human health, agriculture, and food industries. This review comprehensively reviews recent information on the synthesis of valuable compounds and enzymes through fermentation processes. Here, we discuss the advantages of the different types of fermentation, such as submerged and solid-state fermentation, in optimizing metabolite production by bacteria, fungi, and yeast. The role of microbial metabolism, enzymatic activity, and fermentation conditions in enhancing the bioavailability and functionality of these compounds is discussed. Integrating fermentation with emerging biotechnologies, including metabolic engineering, further enhances yields and specificity. The potential of microbial-derived bioactive compounds in developing functional foods, pharmaceuticals, and eco-friendly agricultural solutions positions fermentation as a pivotal strategy for future biotechnological advancements. Therefore, microbial fermentation is a sustainable tool to obtain high-quality metabolites from different sources that can be used in agriculture, animal, and human health. Full article

Entity alignment (EA) facilitates knowledge fusion by matching semantically identical entities in distinct knowledge graphs (KGs). Existing embedding-based methods rely solely on intrinsic KG facts and often struggle with long-tail entities due to insufficient information. Recently, large language models (LLMs), empowered by rich background knowledge and strong reasoning abilities, have shown promise for EA. However, most current LLM-enhanced approaches follow the in-context learning paradigm, requiring multi-round interactions with carefully designed prompts to perform additional auxiliary operations, which leads to substantial computational overhead. Moreover, they fail to fully exploit the complementary strengths of embedding-based small models and LLMs. To address these limitations, we propose HIEA, a novel hierarchical inference framework for entity alignment. By instruction-tuning a generative LLM with a unified and concise prompt and a knowledge adapter, HIEA produces alignment results with a single LLM invocation. Meanwhile, embedding-based small models not only generate candidate entities but also support the LLM through data augmentation and certainty-aware source entity classification, fostering deeper collaboration between small models and LLMs. Extensive experiments on both standard and highly heterogeneous benchmarks demonstrate that HIEA consistently outperforms existing embedding-based and LLM-enhanced methods, achieving absolute Hits@1 improvements of up to 5.6%, while significantly reducing inference cost. Full article

Normalization is a critical step in Multiple-Criteria Decision Analysis (MCDA) because it transforms heterogeneous criterion values into comparable information. This study examines normalization techniques through the lens of entropy, highlighting how criterion data structure shapes normalization behavior and ranking stability within TOPSIS (Technique for Order Preference by Similarity to Ideal Solution). Seven widely used normalization procedures are analyzed regarding mathematical properties, sensitivity to extreme values, treatment of benefit and cost criteria, and rank reversal. Normalization is treated as a source of uncertainty in MCDA outcomes, as different schemes can produce divergent rankings under identical decision settings. Shannon entropy is employed as a descriptive measure of information dispersion and structural uncertainty, capturing the heterogeneity and discriminatory potential of criteria rather than serving as a weighting mechanism. An illustrative experiment with ten alternatives and four criteria (two high-entropy, two low-entropy) demonstrates how entropy mediates normalization effects. Seven normalization schemes are examined, including vector, max, linear Sum, and max–min procedures. For vector, max, and linear sum, cost-type criteria are treated using either linear inversion or reciprocal transformation, whereas max–min is implemented as a single method. This design separates the choice of normalization form from the choice of cost-criteria transformation, allowing a cleaner identification of their respective contributions to ranking variability. The analysis shows that normalization choice alone can cause substantial differences in preference values and rankings. High-entropy criteria tend to yield stable rankings, whereas low-entropy criteria amplify sensitivity, especially with extreme or cost-type data. These findings position entropy as a key mediator linking data structure with normalization-induced ranking variability and highlight the need to consider entropy explicitly when selecting normalization procedures. Finally, a practical entropy-based method for choosing normalization techniques is introduced to enhance methodological transparency and ranking robustness in MCDA. Full article

The traditional cost estimation process in construction involves extracting information from diverse data sources and relying on human intuition and judgment, making it time-intensive and error-prone. While recent advancements in large language models offer opportunities to automate these processes, their effectiveness in cost estimation tasks remains underexplored. Prior studies have investigated LLM applications in construction, but there is a lack of studies that have systematically evaluated their performance in cost estimation or proposed a framework for systematic evaluations of their performance in cost estimation and ways to enhance their accuracy and reliability through prompt engineering. This study evaluates the performance of pre-trained LLMs (GPT-4o, LLaMA 3.2, Gemini 2.0, and Claude 3.5 Sonnet) for conceptual cost estimation, comparing zero-shot prompting with a modular chain-of-thought framework. The results indicate that zero-shot prompting produced incomplete responses with an average confidence score of 1.91 (64%), whereas the CoT framework improved accuracy to 2.52 (84%) and achieved significant gains across BLEU, ROUGE-L, METEOR, content overlap, and semantic similarity metrics. The proposed modular CoT framework enhances structured reasoning, contextual alignment, and reliability in estimation workflows. This study contributes by developing a conceptual cost estimation framework for LLMs, benchmarking baseline model performance, and demonstrating how structured prompting improves estimation accuracy. This offers a scalable foundation for integrating AI into construction cost estimation workflows. Full article

Remote Indigenous communities experience persistent inequities in access to fresh and nutritious foods due to the fragility of perishable food supply chains (PFSCs). Disruptions across procurement, transportation, storage, retail, and limited local production restrict access to perishable foods, contributing to food insecurity and diet-related health risks. This scoping literature review synthesizes evidence from 84 peer-reviewed, grey, and unpublished sources across fourteen countries to map PFSC management (PFSCM) challenges affecting food access in remote Indigenous communities worldwide and to synthesize reported practices implemented to address these challenges. PFSCM challenges were identified across all supply chain levels, and five categories of reported practices emerged: PFSC redesign strategies, forecasting and decision-support models, technological innovations, collaboration and coordination mechanisms, and targeted investments. These findings informed the development of a multi-scalar conceptual framework comprising seven interconnected PFSCM clusters that organize how reported practices are associated with multiple food access dimensions, including quantity, affordability, quality, safety, variety, and cultural acceptability. This review contributes an integrative, system-oriented synthesis of PFSCM research and provides a conceptual basis to support future scholarly inquiry, comparative inquiry, and policy-relevant discussion of food access and health equity in remote Indigenous communities. Full article

Wheel load is a critical information source reflecting the status of vehicle load distribution and motion. Yet, existing in-wheel motor products are primarily designed as propulsion units and inherently lack the load-sensing capabilities required by intelligent vehicles. To address this research gap, this paper presents a novel intelligent electric drive wheel (i-EDW) with an integrated transmission system and a load-sensing unit (LSU). The i-EDW adopts an Axial Flux Permanent Magnet Synchronous Motor (AFPMSM), while the integrated LSU ensures high-precision measurement of six-dimensional wheel forces and moments. According to this multi-axis force information, a real-time estimation and stability control method based on the tire–road friction circle concept is proposed. Instead of the complex decoupling and multi-objective optimization with the multi-actuator systems, this paper focuses on minimizing the tire load rate of i-EDWs, which significantly advances the state of the art in terms of calculation efficiency and respond speed. To validate this theoretical framework, a full-vehicle model equipped with four i-EDWs is developed. In the MATLAB R2022A/Simulink co-simulation environment, a virtual prototype is tested under typical driving scenarios, including the straight-line acceleration and double-moving-lane (DML) steering. The simulation results prove a reliable safety margin from the friction circle boundaries, laying a solid foundation for precise motion control and improved system robustness in future intelligent vehicles. Full article

Whole-genome sequencing is a powerful approach for exploring genomic diversity in livestock species. Chickens (Gallus gallus) are an important food source worldwide, and in Taiwan, poultry production contributes substantially to the livestock industry. Taiwan’s commercial red- and black-feathered country chickens dominate this category and play a crucial role in local poultry production. However, fundamental genomic information on their population structure remains limited. To address this gap, this study generated whole-genome sequencing data from red-feathered country chickens originating from four major breeding farms. Genetic diversity analyses revealed uniformly low genetic diversity across all farms. Runs of homozygosity (ROH) analyses indicated predominantly historical inbreeding, with farm-specific differences in recent inbreeding patterns. Population structure analyses revealed clear clustering of individuals according to farm origin, indicating distinct line structures among breeding farms. These results provide the first comprehensive genomic overview of Taiwan’s commercial red-feather country chickens and offer valuable reference information for future breeding strategies and the development of new lines. Full article

We present a new method for mapping global gas flaring using a multiyear spatio-temporal database of VIIRS Nightfire (VNF) nighttime infrared detections from the Suomi NPP, NOAA-20, and NOAA-21 satellites. The method is designed to resolve closely spaced industrial combustion sources and to produce a stable, physically meaningful flare catalog suitable for long-term monitoring and emissions analysis. The method combines adaptive spatial aggregation of high-temperature detections with a hierarchical clustering that super-resolves individual flare stacks within oil and gas fields. Post-processing yields physically consistent flare footprints and attraction regions, allowing separation of closely spaced sources. Flare clusters are assigned to operational categories (e.g., upstream, midstream, LNG) using prior catalogs combined with AI-assisted expert interpretation. In this step, a multimodal large language model (LLM) provides contextual classification suggestions based on geospatial information, high-resolution daytime imagery, and detection time-series summaries, while final attribution is performed and validated by domain experts. Compared with annual flare catalogs commonly used for national flaring estimates, the new catalog demonstrates substantially improved performance. It is more selective in the presence of intense atmospheric glow from large flares, identifies approximately twice as many active flares, and localizes individual stacks with ~50 m precision, resolving emitters separated by ~400–700 m. For the well-defined class of downstream flares at LNG export facilities, the catalog achieves complete detectability. These improvements support more accurate flare inventories, facility-level attribution, and policy-relevant assessments of gas flaring activity. Full article

Crisis events generate massive information flows from diverse sources, which need to be consolidated into public opinion reports to enable timely response by governments and enterprises. Current LLMs, despite strong generation capabilities, fail to achieve perspective diversity, maintain factual consistency, and perform coherent high-level planning. To address these gaps, we propose MPSR: a multi-perspective self-reflection framework. Our framework first assigns diverse stakeholder personas to agents who independently generate initial writing plans from complementary viewpoints. Subsequently, a three-stage debate mechanism refines these plans by identifying conflicts, formulates resolution strategies, and produces a consensus plan, thereby enhancing factual consistency. Finally, we introduce a Report Fusion mechanism to synthesize reports across temporal batches, ensuring comprehensive event coverage. Extensive experiments demonstrate that MPSR significantly outperforms baselines, achieving Date F1 of 0.67, G-Eval of 4.54, and MiniCheck score of 79.43, which represent improvements of 17.5%, 70.0%, and 25.8% over the strongest baseline, respectively. Full article

Despite a growing number of Earth Observation satellites, a critical observational gap persists for timely, high-resolution flood mapping, primarily due to infrequent satellite revisits and persistent cloud cover. To address this issue, we propose a novel framework that synergistically fuses complementary data from three public sensor types. Our methodology harmonizes these disparate data sources by using surface water fraction as a common variable and downscaling them with flood susceptibility and topography information. This allows for the integration of sub-daily observations from the Visible Infrared Imaging Radiometer Suite and the Advanced Himawari Imager with the cloud-penetrating capabilities of the Advanced Microwave Scanning Radiometer 2. We evaluated this approach on the February 2022 flood in Brisbane, Australia using an independent ground truth dataset. The framework successfully compensates for the limitations of individual sensors, enabling the consistent generation of detailed, high-resolution flood maps. The proposed method outperformed the flood extent derived from commercial high-resolution optical imagery, scoring 77% higher than the Copernicus Emergency Management Service (CEMS) map in the Critical Success Index. Furthermore, the True Positive Rate was twice as high as the CEMS map, confirming that the proposed method successfully overcame the cloud cover issue. This approach provides valuable, actionable insights into inundation dynamics, particularly when other public data sources are unavailable. Full article

This study examines consumer behavior, preferences, and knowledge regarding honey in Kosovo to inform more effective production, marketing, and policy strategies. Data were collected from 503 respondents through an online questionnaire and analyzed using a combination of artificial neural networks (ANN), decision tree modeling (CHAID), and ordinal logistic regression. The results show a high prevalence of honey consumption, strong preference for locally produced honey, and significant variability in consumer willingness to pay (WTP) based on knowledge, income, and trusted information sources. ANN identified recommendations and product familiarity as primary predictors of WTP, while the decision tree highlighted knowledge and income as key variables for segmentation. The ordinal logistic regression confirmed the importance of perceived quality and product attributes, particularly botanical and geographical origin, in shaping purchasing decisions. The use of complementary statistical models enhanced both predictive power and interpretability. The findings highlight the crucial role of consumer education and trust cues in fostering sustainable honey markets in Kosovo. Full article

The purpose of this study is to determine the optimal location for siting an onshore wind farm on the island of Skyros, thereby maximizing performance and minimizing the project’s environmental impacts. Seven evaluation criteria are defined across various sectors, including environmental and economic sectors, and six criteria weighting methods are applied in combination with four multicriteria decision-making (MCDM) ranking methods for suitable areas, resulting in twenty-four ranking models. The alternatives considered in the analysis were defined through the application of constraints imposed by the Specific Framework for Spatial Planning and Sustainable Development for Renewable Energy Sources (SFSPSD RES), complemented by exclusion criteria documented in the international literature, as well as a minimum area requirement ensuring the feasibility of installing at least four wind turbines within the study area. The correlations between their results are then assessed using the Spearman coefficient. Geographic information systems (GISs) are utilized as a mapping tool. Through the application of the methodology, it emerges that area A9, located in the central to northern part of Skyros, is consistently assessed as the most suitable site for the installation of a wind farm based on nine models combining criteria weighting and MCDM methods, which should be prioritized as an option for early-stage wind farm siting planning. The results demonstrate an absolute correlation among the subjective weighting methods, whereas the objective methods do not appear to be significantly correlated with each other or with the subjective methods. The ranking methods with the highest correlation are PROMETHEE II and ELECTRE III, while those with the lowest are TOPSIS and VIKOR. Additionally, the hierarchy shows consistency across results using weights from AHP, BWM, ROC, and SIMOS. After applying multiple methods to investigate correlations and mitigate their disadvantages, it is concluded that when experts in the field are involved, it is preferable to incorporate subjective multicriteria analysis methods into decision-making problems. Finally, it is recommended to use more than one MCDM method in order to reach sound decisions. Full article

Background: Considering the significant impact on quality of life and the chronic nature of temporomandibular dysfunction (TMD), seeking healthcare is also part of the reality of individuals with this disorder. However, cultural differences and similarities in the experiences of individuals with TMD have not yet been investigated. This study aimed to describe and compare the experiences, beliefs, and sociocultural factors of Brazilian and Spanish individuals with TMD, focusing on their perceptions of the disorder, diagnostic pathways, information-seeking behaviors, and treatment expectations. Methods: A descriptive qualitative study was conducted. A purposive sample of 50 participants (25 Brazilian, 25 Spanish), aged 18–50 and diagnosed with TMD according to DC/TMD criteria, was recruited. Data were obtained through semi-structured interviews and analyzed using thematic analysis. Results: Six themes emerged, revealing both similarities and differences between the groups. Brazilian participants reported uncertainty about which professional to consult and difficulty accessing specialized care. In contrast, Spanish participants frequently sought physical therapists as their first option and identified them as primary sources of information. Beliefs about TMD etiology varied across samples. Treatment expectations also differed. Brazilians emphasized the difficulty of obtaining effective care, while Spanish participants perceived physiotherapy as being limited to muscular disorders. Perceptions of occlusal splint effectiveness showed variation between the groups. Conclusions: These findings underscore the necessity of culturally sensitive approaches to patient care that address not only clinical aspects, but also the sociocultural context that influences health behaviors. Full article

Peatlands play a critical role in global and regional climate regulation by functioning as long-term carbon sinks, regulating hydrology, and modulating land–atmosphere energy exchange. Intact peat ecosystems store large amounts of organic carbon and stabilize local climate through high water retention and evapotranspiration, whereas peatland degradation disrupts these functions and can transform peatlands into significant sources of greenhouse gas emissions and climate extremes such as drought and fire. Indonesia contains approximately 13.6–40.5 Gt of carbon, around 40% of which is stored on the island of Sumatra. However, tropical peatlands in this region are highly vulnerable to climate anomalies and land-use change. This study investigates the impacts of major climate anomalies—specifically El Niño and positive Indian Ocean Dipole (pIOD) events in 1997/1998, 2015/2016, and 2019—on peatland cover change across South Sumatra, Jambi, Riau, and the Riau Islands. Landsat 5 Thematic Mapper and Landsat 8 Operational Land Imager/Thermal Infrared Sensor imagery were analyzed using a Random Forest machine learning classification approach. Climate anomaly periods were identified using El Niño-Southern Oscillation (ENSO) and IOD indices from the National Oceanic and Atmospheric Administration. To enhance classification accuracy and detect vegetation and hydrological stress, spectral indices including the Normalized Difference Vegetation Index (NDVI), Modified Soil Adjusted Vegetation Index (MSAVI), Normalized Difference Water Index (NDWI), and Normalized Difference Drought Index (NDDI) were integrated. The results show classification accuracies of 89–92%, with kappa values of 0.85–0.90. The 2015/2016 El Niño caused the most severe peatland degradation (>51%), followed by the 1997/1998 El Niño (23–38%), while impacts from the 2019 pIOD were comparatively limited. These findings emphasize the importance of peatlands in climate regulation and highlight the need for climate-informed monitoring and management strategies to mitigate peatland degradation and associated climate risks. Full article

Precipitation in high mountain areas is of critical importance as these regions are major sources of freshwater, supporting river basins, ecosystems, and downstream communities. Changes in precipitation regimes in these regions can have cascading impacts on water availability, agriculture, hydropower, and biodiversity. The present study aims to give new information about precipitation variability in high mountain regions of Bulgaria (Musala, Botev Peak, and Cherni Vrah) and to assess the role of large-scale atmospheric circulation patterns for the occurrence of extreme precipitation months. The study period is 1937–2024, and the classification of extreme precipitation months is based on the 10th and 90th percentiles of precipitation distribution. The temporal distribution of extreme precipitation months was analyzed by comparison of two periods (1937–1980 and 1981–2024). The impact of atmospheric circulation was evaluated by correlation between the number of extreme precipitation months and indices for the North Atlantic Oscillation (NAO) and Western Mediterranean Oscillation (WeMO). Results show a statistically significant decrease in winter and spring precipitation at Musala and Cherni Vrah, and a persistent drying tendency at Cherni Vrah across all seasons. The frequency of extremely wet months in winter and autumn has sharply declined since 1981, whereas extremely dry months have become more common, particularly during the cold season. Precipitation erosivity also exhibits station-specific responses, with Musala and Cherni Vrah showing reduced monthly concentration, while Botev Peak retains pronounced warm-season erosive rainfall. Circulation analysis indicates that positive NAOI phases favor dry extremes, while positive WeMOI phases enhance wet extremes. These findings reveal a shift toward drier and more seasonally uneven conditions in Bulgaria’s alpine zone, increasing hydrological risks related to drought, water scarcity, and soil erosion. The identified shifts in precipitation seasonality and intensity offer essential guidance for forecasting hydrological risks and mitigating soil erosion in vulnerable mountain ecosystems. The study underscores the need for adaptive water-resource strategies and enhanced monitoring in high-mountain areas. Full article