Search Results (311)

Free-roaming domestic cats (Felis catus) present a major management challenge for animal welfare and biodiversity conservation. Trap-neuter-return (TNR) programs, which include sterilization and return of cats, are increasingly adopted to manage cat populations, often alongside routine food provisioning. However, their effectiveness in reducing cats’ reliance on wild prey remains contested. In this study, we use stable isotope analysis (δ¹³C, δ¹⁵N, δ³⁴S) of cat fur to investigate dietary patterns before and after TNR implementation in the context of concurrent changes in food availability linked to the closure of nearby mink-farming operations. We analyzed samples from 122 cats in a large-scale TNR initiative on a rural property in British Columbia, Canada. These included indoor cats (control), free-roaming cats prior to TNR (Group 1), a subset of Group 1 re-sampled months after food provisioning began (Run 2), and newly sampled cats that had been fed regularly before trapping (Group 2). Local prey and food sources were also analyzed to provide a comparative isotopic baseline. Our results show clear dietary shifts following TNR. Group 1 cats exhibited high isotopic variability and elevated δ¹⁵N and δ³⁴S values, consistent with wild prey consumption. In contrast, post-TNR cats showed significantly lower and more homogeneous values, aligning closely with those of indoor, kibble-fed cats. These changes are consistent with a reduced dietary reliance on wildlife and raw mink feed following the combination of TNR with regular provisioning and the cessation of mink operations. These findings demonstrate that regular food provisioning in TNR-managed colonies, particularly when combined with broader environmental changes, can significantly alter cat diets and potentially reduce their dependence on wild prey. Full article

The health and productivity of rabbits are highly sensitive to the environmental conditions within the rabbit house, particularly to fluctuations and deviations in temperature, relative humidity, and carbon dioxide (CO₂) concentration. However, owing to the thermal inertia and residual evaporation effects inherent in ventilation and cooling systems, environmental changes often exhibit delayed responses, rendering real-time control inadequate. Accurate prediction of key environmental parameters is indispensable for formulating effective environmental control strategies, as it enables consideration of their future dynamics and thereby enhances the rationality of regulation in rabbit farming. Existing prediction models often exhibit unsatisfactory accuracy and weak generalization, which restricts the incorporation of prediction into effective environmental control strategies. To address these limitations, summer indoor and outdoor environmental data were collected from rabbit houses in Nanping, Fujian; Jiyuan, Henan; and Qingyang, Gansu, China—three climatically distinct regions—forming three datasets. Based on these datasets, a multi-parameter time-series prediction model, Patch and Cross-Attention Enhanced Transformer for Rabbit House Prediction (PatchCrossFormer-RHP), is introduced, integrating patching and attention mechanisms. The model partitions the sequences of rabbit house temperature, relative humidity, and CO₂ concentration into patches and incorporates auxiliary parameters, such as indoor air velocity and outdoor temperature and humidity, to enhance feature representation. Furthermore, it applies cross-attention with differentiated encoding to disentangle multi-parameter relationships and improve predictive performance. This study used the Fujian dataset as the primary benchmark. On this dataset, PatchCrossFormer-RHP achieved root mean square error (RMSE) values of 0.290 °C, 1.554%, and 38.837 ppm for rabbit house temperature, humidity, and CO₂ concentration, respectively, with corresponding R² values of 0.963, 0.956, and 0.838, consistently outperforming RNN, GRU, and LSTM. Transfer experiments with single- and multi-source pretraining followed by fine-tuning on Fujian demonstrated that strong cross-regional generalization can be achieved with only limited target-domain data. Full article

Controlled environment agriculture (CEA) pivots food production from an outdoor field setting to the indoors where growing conditions can be calibrated to fit crop needs. This research investigates vertical farms as a type of CEA. In particular, using the St. Louis area as a case study, it provides data-driven support for optimizing a vertical farm’s business model including its supply chain. The methodology presented here informs agri-preneurs about what crops to grow in a vertical farm, how much to grow given local market demand, and what vertical farm configuration (e.g., Dutch bucket, nutrient film technique, deep water culture) a facility should use. Based on the case study’s base scenario, the simulated vertical farm business would record an economic loss. However, the study did find several paths to improving profitability. First, reducing fixed and variable costs benefits profitability. Proper facility-level production and resource planning helps with managing the fixed costs. Second, increasing market prices may benefit profitability, but it has diminishing returns. As a result, firms can justify making investments that enhance their reputation and market competitiveness, though the advantage these marketing activities provide will decline as prices increase. Third, growing demand or increasing market share does not necessarily improve profitability. Full article

This study evaluated the performance of a hybrid heat pump system integrating photovoltaic–thermal (PVT) panels with a standing column well (SCW) geothermal system in a strawberry greenhouse. The PVT panels, installed over 10% of the area of a 175 m³ greenhouse, stored excess solar heat in an aquifer to offset the reduced efficiency of the geothermal source during extended operation. The results showed that the hybrid system can supply 11,253 kWh of heat energy during the winter, maintaining the night time indoor temperature at 10 °C even when outdoor conditions dropped to −10.5 °C. The PVT system captured 11,125 kWh of solar heat during heating the off season, increasing the heat supply up to 22,378 kWh annually. Additionally, the system generated 3839 kWh of electricity, which significantly offset the 36.72% of the annual pump system electricity requirements, enhancing the system coefficient of performance (COP) of 3.38. Strawberry production increased by 4% with 78% heating cost saving compared to a kerosene boiler system. The results show that the PVT system effectively supports the geothermal system, improving heating performance and demonstrating the feasibility of hybrid renewable energy in smart farms to enhance efficiency, reduce fossil fuel use, and advance carbon neutrality. Full article

According to United Nations projections, the global population is expected to reach 9.7 billion by 2050, with 70% residing in urban areas, while arable land availability continues to decline. Vertical farming (VF) offers a promising pathway for sustainable urban food production by utilizing vertical space and controlled environments. Among emerging approaches, the adaptive vertical farm (AVF) introduces movable shelving systems that adjust to plant growth stages, allowing a higher number of cultivation shelves to be accommodated within the same rack height. In this study, we developed a computational model to quantify and compare the energy consumption of AVF and conventional VF systems under industrial-scale conditions. The reference scenario considered 272 multilevel racks, each hosting 8 shelves in the VF and 15 shelves in the AVF, with Lactuca sativa as the test crop. Energy consumption for thermohygrometric control and lighting was estimated under different sowing schedules, with crop growth dynamics simulated using scheduling algorithms. Plant heat loads were calculated through the Penman–Monteith model, enabling a robust estimation of evapotranspiration and its impact on indoor climate control. Simulation results show that the AVF achieves an average 22% reduction in specific energy consumption for climate control compared to the VF, independently of sowing strategies. Moreover, the AVF nearly doubles the number of cultivation shelves within the same footprint, increasing the cultivable surface area by over 400% compared to traditional flat indoor systems. This work provides the first quantitative assessment of AVF energy performance, demonstrating its potential to simultaneously improve land-use efficiency and reduce energy intensity, thereby supporting the sustainable integration of vertical farming in urban food systems. Full article

Solar greenhouses are an important component of modern facility agriculture, and the dynamic changes in their internal environment directly affect crop growth and yield. Among these factors, crop transpiration releases water vapor through transpiration, directly altering the indoor humidity balance and forming a dynamic coupling with factors such as temperature and light. The environment of solar greenhouses exhibits highly nonlinear and multivariate coupling characteristics, leading to insufficient prediction accuracy in existing models. However, accurate predictions are crucial for regulating crop growth and yield. However, current mainstream greenhouse environmental prediction models still have obvious limitations when dealing with such complexity: traditional machine learning models and single-variable-driven models have issues such as insufficient accuracy (average MAE is 15–20% higher than in this study) and weak adaptability to nonlinear environmental changes in multi-environmental factor coupling predictions, making it difficult to meet the needs of precision farming. A review of relevant research over the past five years shows that while LSTM-based models perform well in time series prediction, they ignore the spatial correlations between environmental factors. Models incorporating attention mechanisms can capture key variables but suffer from high computational costs. To address these issues, this study proposes a prediction model based on multi-strategy optimization and gradient-boosting (GBDT) algorithms. By introducing a multi-scale feature fusion module, it addresses the accuracy issues in multi-factor coupling prediction. Additionally, it employs a lightweight network design to balance prediction performance and computational efficiency, filling the gap in existing research applications under complex greenhouse environments. The model optimizes data preprocessing and model parameters through Sobol sequence initialization, adaptive t-distribution perturbation strategies, and Gaussian–Cauchy mixture mutation strategies and combines CatBoost for modeling to enhance prediction accuracy. Experimental results show that the MSCSO–CatBoost model performs excellently in temperature prediction, with the mean absolute error (MAE) and root mean square error (RMSE) reduced by 22.5% (2.34 °C) and 24.4% (3.12 °C), respectively, and the coefficient of determination (R²) improved to 0.91, significantly outperforming traditional regression methods and combinations of other optimization algorithms. Additionally, the model demonstrates good generalization capability in predicting multiple environmental variables such as temperature, humidity, and light intensity, adapting to environmental fluctuations under different climatic conditions. This study confirms that combining multi-strategy optimization with gradient-boosting algorithms can significantly improve the prediction accuracy of solar greenhouse environments, providing reliable support for precision agricultural management. Future research could further explore the model’s adaptive optimization in complex climatic regions. Full article

Particulate matter (PM) is a significant air pollutant in modern egg production. However, high-quality PM data from commercial egg farms are still very limited. A 6-month study, covering both cold and hot seasons, measured PM₁₀ concentrations and emissions in a 140,000-hen commercial laying hen house in the Midwest USA. An advanced measurement system was implemented for continuous and real-time monitoring, collecting data from 67 online instruments and sensors. The study generated 4318 h of valid PM₁₀ data, with 97.8% data completeness. The average daily mean (ADM) PM₁₀ concentration in the house exhaust air, standardized to 20 °C and 1 atm, was 236 ± 162 (ADM ± standard deviation) µg m⁻³. The ADM net PM₁₀ emission was 18.9 ± 2.2 mg d⁻¹ hen⁻¹. Increasing outdoor temperatures were correlated with decreased indoor PM₁₀ concentrations but increased overall emissions. Comparison with the ADM emission of 12.4 ± 13.3 mg d⁻¹ hen⁻¹ from the same house during a previous six-month study in 2004–2005 revealed that artificial hen molting in this study increased PM₁₀ concentrations and emissions. Extrapolating the ADM PM₁₀ emission from the house, the ADM PM₁₀ emission from the entire egg farm was estimated at 35.6 ± 31.1 kg d⁻¹ (or 35.6 ± 4.5 kg d⁻¹ with a 95% confidence interval). This study provides valuable insights into air quality in animal agriculture and contributes high-quality and real-world data for use in data-driven approaches such as artificial intelligence, machine learning, data mining, and big data analytics. Full article

In this study, a new approach was developed for the estimation of optimum parameters (ODP), in terms of materials and design in livestock barns, and for optimal design. For this purpose, two thousand simulations were run using Monte Carlo (MC) techniques and Latin hypercube methods using the Energy Plus program on a 50-head closed dairy farm. In this study, the heat balance in the barn was adapted to Energy Plus using an innovative approach, using heat balance equations according to the ASHRAE Standard. First, data normality was determined using the Shapiro–Wilk (SW) and Kolmogorov–Smirnov (KS) tests. Data on thermal stress duration and energy consumption for dairy cattle welfare were estimated directly from the simulations, and sensitivity (SA) and uncertainty (UA) analyses were conducted. Furthermore, the statistical relationship between thermal comfort and energy consumption was determined using Pearson correlation. The predicted values obtained from the simulations were validated with barn values, and time-series overlay plots and histograms were generated. Furthermore, interpretations of the validation processes were made based on MBE, RSME, and R² statistical values. The study estimated an indoor thermal comfort temperature of 12 °C, and this value was taken into account in the innovatively developed simulations. The estimated optimum design parameters in the study resulted in energy reductions of 25% and 41% for walls and roofs, 48% and 19% for cooling and heating setpoint temperatures, 43% and 37% for window areas, and 75% and 40% for natural and mechanical ventilation, respectively. When the design parameters were evaluated holistically and analyzed in terms of average values, the new simulation model achieved approximately 50% energy savings. We believe that the newly developed approach will guide future planning for countries, the public, and private sectors to ensure animal welfare and reduce energy consumption. Full article

Consumer demand for high-quality products, combined with expectations for more sustainable production systems and animal welfare, is driving major changes in livestock farming practices. It is known that space allowance plays a central role in pig welfare, promoting resting and reducing the incidence of injuries and stress-related behaviors; however, there is little scientific evidence on the effect that available space has on the carcass and meat quality. In this study, space allowances were compared, in both an indoor conventional system (1.15, 1.9 and 3 m²/pig) and an indoor organic system with outdoor access (1.4 + 1, 2.6 + 2 and 3.9 + 3 m²/pig). The increase in space available for pigs had no effect on pig performance, carcass and meat quality characteristics, such as pH, drip and cooking loss. However, lowering stocking density in the conventional indoor housing system improved meat tenderness, as assessed by the Slice Shear Force test, while no difference was found between meat tenderness in organic pigs raised with three different stocking densities. Increased space allowance per pig reduced n-3 fatty acids in pig loins from both housing systems and n-6 fatty acids and PUFAs in loins from pigs reared in the organic housing system with both indoor and outdoor space. Full article

Continuous and accurate biomass measurement is a critical enabler for control, decision making, and optimization in modern plant production systems. It supports the development of plant growth models for advanced control strategies like model predictive control, and enables responsive, data-driven, and plant state-dependent cultivation. Traditional biomass measurement methods, such as destructive sampling, are time-consuming and unsuitable for high-frequency monitoring. In contrast, image-based estimation using computer vision and deep learning requires frequent retraining and is sensitive to changes in lighting or plant morphology. This work introduces a low-cost, load-cell-based biomass monitoring system tailored for vertical farming applications. The system operates at the level of individual growing trays, offering a valuable middle ground between impractical plant-level sensing and overly coarse rack-level measurements. Tray-level data allow localized control actions, such as adjusting light spectrum and intensity per tray, thereby enhancing the utility of controllable LED systems. This granularity supports layer-specific optimization and anomaly detection, which are not feasible with rack-level feedback. The biomass sensor is easily scalable and can be retrofitted, addressing common challenges such as mechanical noise and thermal drift. It offers a practical and robust solution for biomass monitoring in dynamic, growing environments, enabling finer control and smarter decision making in both commercial and research-oriented vertical farming systems. The developed sensor was tested and validated against manual harvest data, demonstrating high agreement with actual plant biomass and confirming its suitability for integration into vertical farming systems. Full article

Indoor horticulture requires a substantial quantity of electricity to meet crops extended photoperiodic requirements for optimal photosynthetic rate. Simultaneously, global electricity costs have grown dramatically in recent years, endangering the sustainability and profitability of indoor vertical farms and/or modern greenhouses that use artificial lighting systems to accelerate crop development and growth. This study investigates the growth rate and physiological development of cherry tomato plants cultivated in a pilot indoor vertical farm at the Agricultural University of Athens’ Laboratory of Farm Structures (AUA) under continuous and disruptive lighting. The leaf physiological traits from multiple photoperiodic stress treatments were analyzed and utilized to estimate the plant’s tolerance rate under varied illumination conditions. Four different photoperiodic treatments were examined and compared, firstly plants grew under 14 h of continuous light (C-14L10D/control), secondly plants grew under a normalized photoperiod of 14 h with intermittent light intervals of 10 min of light followed by 50 min of dark (NI-14L10D/stress), the third treatment where plants grew under 14 h of a load-shifted energy demand response intermittent lighting schedule (LSI-14L10D/stress) and finally plants grew under 13 h photoperiod following of a load-shifted energy demand response intermittent lighting schedule (LSI-13L11D/stress). Plants were subjected also under two different light spectra for all the treatments, specifically WHITE and Blue/Red/Far-red light composition. The aim was to develop flexible, energy-efficient lighting protocols that maintain crop productivity while reducing electricity consumption in indoor settings. Results indicated that short periods of disruptive light did not negatively impact physiological responses, and plants exhibited tolerance to abiotic stress induced by intermittent lighting. Post-harvest data indicated that intermittent lighting regimes maintained or enhanced growth compared to continuous lighting, with spectral composition further influencing productivity. Plants under LSI-14L10D and B/R/FR spectra produced up to 93 g fresh fruit per plant and 30.4 g dry mass, while consuming up to 16 kWh less energy than continuous lighting—highlighting the potential of flexible lighting strategies for improved energy-use efficiency. Full article

Urban agriculture (UA) is emerging as a promising strategy for sustainable food production in response to growing environmental pressures. Indoor vertical farming (IVF), combining Controlled Environment Agriculture (CEA) with Building-Integrated Agriculture (BIA), enables efficient resource use and year-round crop cultivation in urban settings. This study assesses the environmental performance of a prospective IVF system located on a university campus in Portugal, focusing on the integration of photovoltaic (PV) energy as an alternative to the conventional electricity grid (GM). A Life Cycle Assessment (LCA) was conducted using the Environmental Footprint (EF) method and the LANCA model to account for land use and soil-related impacts. The PV-powered system demonstrated lower overall environmental impacts, with notable reductions across most impact categories, but important trade-offs with decreased soil quality. The LANCA results highlighted cultivation and packaging as key contributors to land occupation and transformation, while also revealing trade-offs associated with upstream material demands. By combining EF and LANCA, the study shows that IVF systems that are not soil-based can still impact soil quality indirectly. These findings contribute to a broader understanding of sustainability in urban farming and underscore the importance of multi-dimensional assessment approaches when evaluating emerging agricultural technologies. Full article

Accurate pig counting is crucial for precision livestock farming, enabling optimized feeding management and health monitoring. Detection-based counting methods face significant challenges due to mutual occlusion, varying illumination conditions, diverse pen configurations, and substantial variations in pig densities. Previous approaches often struggle with complex agricultural environments where lighting conditions, pig postures, and crowding levels create challenging detection scenarios. To address these limitations, we propose EAPC-YOLO (enhanced adaptive pig counting YOLO), a robust architecture integrating density-aware processing with advanced detection optimizations. The method consists of (1) an enhanced YOLOv8 network incorporating multiple architectural improvements for better feature extraction and object localization. These improvements include DCNv4 deformable convolutions for irregular pig postures, BiFPN bidirectional feature fusion for multi-scale information integration, EfficientViT linear attention for computational efficiency, and PIoU v2 loss for improved overlap handling. (2) A density-aware post-processing module with intelligent NMS strategies that adapt to different crowding scenarios. Experimental results on a comprehensive dataset spanning diverse agricultural scenarios (nighttime, controlled indoor, and natural daylight environments with density variations from 4 to 30 pigs) demonstrate our method achieves 94.2% mAP@0.5 for detection performance and 96.8% counting accuracy, representing 12.3% and 15.7% improvements compared to the strongest baseline, YOLOv11n. This work enables robust, accurate pig counting across challenging agricultural environments, supporting precision livestock management. Full article

Background: Reliable and non-invasive methods for assessing welfare in poultry are essential for improving evidence-based welfare monitoring and advancing management practices in commercial production systems. The iris-to-pupil (IP) ratio, previously validated by our group in primates and cattle, reflects autonomic nervous system balance and may serve as a physiological indicator of stress in laying hens. This study evaluated the utility of the IP ratio under field conditions across diverse commercial layer housing systems. Materials and Methods: In total, 296 laying hens (Lohmann Brown, n = 269; White Leghorn, n = 27) were studied across four locations in Canada housed under different systems: Guelph (indoor; pen), Spring Island (outdoor and scratch; organic), Ottawa (outdoor, indoor and scratch; free-range), and Toronto (outdoor and hobby; free-range). High-resolution photographs of the eye were taken under ambient lighting. Light intensity was measured using the light meter app. The IP ratio was calculated using NIH ImageJ software (Version 1.54p). Statistical analysis included one-way ANOVA and linear regression using GraphPad Prism (Version 5). Results: Birds housed outdoors had the highest IP ratios, followed by those in scratch systems, while indoor and pen-housed birds had the lowest IP ratios (p < 0.001). Subgroup analyses of birds in Ottawa and Spring Island farms confirmed significantly higher IP ratios in outdoor environments compared to indoor and scratch systems (p < 0.001). The IP ratio correlated weakly with ambient light intensity (r² = 0.25) and age (r² = 0.05), indicating minimal influence of these variables. Although White Leghorn hens showed lower IP ratios than Lohmann Browns, this difference was confounded by housing type; all White Leghorns were housed in pens. Thus, housing system but not breed was the primary driver of IP variation. Conclusions: The IP ratio is a robust, non-invasive physiological marker of welfare assessment in laying hens, sensitive to housing environment but minimally influenced by light or age. Its potential for integration with digital imaging technologies supports its use in scalable welfare assessment protocols. Full article

Light quality and biostimulants regulate alkaloid biosynthesis and promote plant growth, but their combined effects on vindoline (VDL) and catharanthine (CAT) production in Catharanthus roseus remain underexplored. This study investigated the impact of different LED spectra and an arbuscular mycorrhizal fungi-based biostimulant (BS) on VDL and CAT production in indoor-grown C. roseus. After a 60-day pretreatment under white LEDs, plants were exposed to eight treatments: white (W, control), red (R), blue (B), and red-blue (RB) light, and their combinations with BS. Samples were collected before treatments (T0) and 92 days after pretreatment (T1). No mycorrhizal development was observed. VDL was detected in both roots and leaves, with higher levels in roots. R produced significantly higher mean concentrations of both VDL and CAT than W. BS significantly increased mean concentrations and total yields of both alkaloids than the untreated condition. The combination of R and BS produced the highest mean concentrations and total yields of VDL and CAT. In particular, it resulted in a significantly higher mean concentration and total yield of VDL compared to sole W. Total yields increased from T0 to T1, primarily due to a substantial rise in root yield. In conclusion, combining R and BS proved to be the most effective strategy to enhance VDL and CAT production by maximizing their total yields, which also increased over time due to greater root contribution. This underscores the importance of combining targeted treatments with harvesting at specific stages to optimize alkaloid production under controlled conditions. Full article