Search Results (1,754)

Museums may acquire archaeological artefacts discovered by non-specialists or amateur archaeologists, holding the potential to promote the safeguarding of cultural heritage by integrating the local community in their activities. However, this also creates an opportunity for the fraudulent sale of modern forgeries presented as archaeological artefacts, resulting in the need for a critical assessment of the artefact’s authenticity prior to acquisition by the museum. In 2019, the regional museum in Cáceres (Spain) was offered the opportunity to acquire a Phoenician-Punic head pendant, allegedly discovered in the vicinity of the city. The artefact’s authenticity was assessed by traditional approaches, including typological analysis and analysis of manufacture technique, which raised doubts about its purported age. VP-SEM-EDS analysis of the chemical composition of the different glass portions comprising the pendant was used for non-invasive determination of glassmaking recipes, enabling the identification of glass components incompatible with known Iron Age glassmaking recipes from the Mediterranean. Further comparison with historical and modern glassmaking recipes allowed for the identification of the artefact as a recent forgery made from glasses employing modern colouring and opacifying techniques. Full article

Leishmaniasis is an infectious disease common in tropical and subtropical regions worldwide. This study aimed to develop a topical meglumine antimoniate gel (MA-gel) for the treatment of cutaneous leishmaniasis. The MA-gel was characterized in terms of morphology, pH, swelling, porosity, rheology, and thermal properties by differential scanning calorimetry (DSC). Biopharmaceutical evaluation included in vitro drug release and ex vivo skin permeation. Safety was evaluated through biomechanical skin property measurements and cytotoxicity in HaCaT and RAW 267 cells. Leishmanicidal activity was tested against promastigotes and amastigotes of Leishmania infantum, and in silico studies were conducted to explore possible mechanisms of action. The composition of the MA-gel included 30% MA, 20% Pluronic^® F127 (P407), and 50% water. Scanning electron microscopy revealed a sponge-like and porous internal structure of the MA-gel. This formula exhibited a pH of 5.45, swelling at approximately 12 min, and a porosity of 85.07%. The DSC showed that there was no incompatibility between MA and P407. Drug release followed a first-order kinetic profile, with 22.11 µg/g/cm² of the drug retained in the skin and no permeation into the receptor compartment. The MA-gel showed no microbial growth, no cytotoxicity in keratinocytes, and no skin damage. The IC₅₀ for promastigotes and amastigotes of L. infantum were 3.56 and 23.11 µg/mL, respectively. In silico studies suggested that MA could act on three potential therapeutic targets according to its binding mode. The MA-gel demonstrated promising physicochemical, safety, and antiparasitic properties, supporting its potential as a topical treatment for cutaneous leishmaniasis. Full article

Cellulose acetate (CA), commercially produced from natural cellulose, is one of the promising candidates to solve the microplastic issue. In this study, attempts were made to prepare CA microparticles by means of melt extrusion of incompatible polymer blends comprising CA with plasticizer (triacetin (TA)) and polyvinyl alcohol (PVA) followed by selective removable of TA and PVA. As implied by semi-theoretical equation previously established by Wu (Wu’s equation), particle size decreased with increasing shear rate or decreasing viscosity ratio of polymers. CA microparticles with a controlled size of 2–8 μm, narrow particle size distribution, and smooth surface were successfully obtained. Efforts were made to determine the numerical solution of Wu’s equation to compare them with observed particle size. To this end, interfacial tension between dispersed and matrix phases to be incorporated in the equation was determined by group contribution methods. The root mean squared error (RMSE) between the observed and calculated particle size was unsatisfactorily large, 4.46 μm. It was found that one of the possible reasons for the limited prediction accuracy was migration of TA from the dispersed to matrix phase affecting the viscosity ratio. Further efforts will be required to achieve a better prediction. Full article

Climate change is jeopardizing global food security, with at least 713 million people facing hunger. To face this challenge, legumes as common beans could offer a nature-based solution, sourcing nutrients and dietary fiber, especially for rural communities in Latin America and Africa. However, since common beans are generally heat and drought susceptible, it is imperative to speed up their molecular introgressive adaptive breeding so that they can be cultivated in regions affected by extreme weather. Therefore, this study aimed to couple an advanced panel of common bean (Phaseolus vulgaris L.) × tolerant Tepary bean (P. acutifolius A. Gray) interspecific lines with Bayesian regression algorithms to forecast adaptation to the humid and dry sub-regions at the Caribbean coast of Colombia, where the common bean typically exhibits maladaptation to extreme heat waves. A total of 87 advanced lines with hybrid ancestries were successfully bred, surpassing the interspecific incompatibilities. This hybrid panel was genotyped by sequencing (GBS), leading to the discovery of 15,645 single-nucleotide polymorphism (SNP) markers. Three yield components (yield per plant, and number of seeds and pods) and two biomass variables (vegetative and seed biomass) were recorded for each genotype and inputted in several Bayesian regression models to identify the top genotypes with the best genetic breeding values across three localities on the Colombian coast. We comparatively analyzed several regression approaches, and the model with the best performance for all traits and localities was BayesC. Also, we compared the utilization of all markers and only those determined as associated by a priori genome-wide association studies (GWAS) models. Better prediction ability with the complete SNP set was indicative of missing heritability as part of GWAS reconstructions. Furthermore, optimal SNP sets per trait and locality were determined as per the top 500 most explicative markers according to their β regression effects. These 500 SNPs, on average, overlapped in 5.24% across localities, which reinforced the locality-dependent nature of polygenic adaptation. Finally, we retrieved the genomic estimated breeding values (GEBVs) and selected the top 10 genotypes for each trait and locality as part of a recommendation scheme targeting narrow adaption in the Caribbean. After validation in field conditions and for screening stability, candidate genotypes and SNPs may be used in further introgressive breeding cycles for adaptation. Full article

Cement-based building materials usually exhibit weak flexural behavior under high temperature or fire conditions. This paper develops a novel geopolymer with enhanced residual flexural strength, incorporating fly ash/metakaolin precursors and corundum aggregates based on our previous study, and further improves flexural performance using hybrid fibers. The flexural load–deflection response, strength, deformation capacity, toughness and microstructure are investigated by a thermal exposure test, bending test and microstructure observation. The results indicate that the plain geopolymer exhibits a continuously increasing flexural strength from 10 MPa at 20 °C to 25.9 MPa after 1000 °C exposure, attributed to thermally induced further geopolymerization and ceramic-like crystalline phase formation. Incorporating 5% wollastonite fibers results in slightly increased initial and residual flexural strength but comparable peak deflection, toughness and brittle failure. The binary 5% wollastonite and 1% basalt fibers in geopolymer obviously improve residual flexural strength exposed to 400–800 °C. The steel fibers show remarkable reinforcement on flexural behavior at 20–800 °C exposure; however, excessive steel fiber content such as 2% weakens flexural properties after 1000 °C exposure due to severe oxidation deterioration and thermal incompatibility. The wollastonite/basalt/steel fibers exhibit a positive synergistic effect on flexural strength and toughness of geopolymers at 20–600 °C. Full article

In a society that advocates for human rights, a moralistic, needs-based approach is incompatible with policies and practices that ensure educational inclusion for all individuals regardless of gender identity or sexual orientation. This study analyses the perspectives of members of the educational community on sex–gender diversity. Specifically, it presents the views of 32 individuals from four primary schools located in southern Andalusia. A qualitative methodology based on interviews and content analysis was employed, supported by NVIVO12 software. The findings reveal differing understandings of sex–gender diversity; the school’s stance on such diversity and, particularly, on LGBTIQ+phobia; as well as the role of initial teacher training in this area. Full article

Segregative phase separation technology demonstrates substantial potential for precise molecular fractionation in food and biomaterial applications. The investigation elucidates the causal relationship between viscosity variations and phase separation dynamics, which govern molecular fractionation in GA/HPMC composite systems. By conducting a comparative analysis of two GA subtypes (CGA and SGA) and three HPMC grades with controlled viscosity gradients, we utilized gel permeation chromatography-multi-angle laser light scattering (GPC-MALLS) coupled with rheological characterization to elucidate the critical relationship between continuous phase viscosity and fractionation efficiency. Notably, increasing HPMC viscosity significantly intensified phase separation, resulting in selective enrichment of arabinogalactan-protein complexes: from 6.3% to 8.5% in CGA/HPMC systems and from 27.3% to 36.5% in SGA/HPMC systems. Further mechanistic investigation revealed that elevated HPMC viscosity enhances thermodynamic incompatibility while slowing interfacial mass transfer, synergistically driving component redistribution. These findings establish a quantitative viscosity–fractionation relationship, offering theoretical insights for optimizing GA/HPMC systems in emulsion stabilization, microencapsulation, and functional biopolymer purification via viscosity-mediated phase engineering. Full article

Alpha-Synuclein (α-Syn) is a presynaptic neuronal protein implicated in the pathogenesis of Parkinson’s disease (PD) and other synucleinopathies, primarily through its aggregation into insoluble fibrils. The extended α-Syn half-life necessitates treatment durations that are incompatible with efficient high-throughput drug screening, can risk compound stability or cause cellular toxicity. To address this, we inserted a PEST sequence, a motif known to promote rapid protein degradation, at the C-terminus of the SNCA gene using CRISPR/Cas9 to create a novel cell line with reduced α-Syn half-life. This modification accelerates α-Syn turnover, providing a robust model for studying α-Syn dynamics and offering a platform that is applicable to other long-lived proteins. Our results demonstrate a six-fold reduction in α-Syn half-life, enabling the rapid detection of changes in protein levels and facilitating the identification of molecules that modulate α-Syn production and degradation pathways. Using inhibitors of the proteasome, transcription, and translation further validated the model’s utility in examining various mechanisms that impact protein levels. This novel cell line represents a significant advancement for studying α-Syn dynamics and offers promising avenues to develop therapeutics for α-synucleinopathies. Future research should focus on validating this model in diverse experimental settings and exploring its potential in high-throughput screening applications. Full article

Ultra-high-performance concrete (UHPC) jackets present a promising solution for enhancing the seismic resilience of seismically deficient reinforced concrete (RC) bridge columns. This study investigates jacket thickness optimization through integrated experimental and numerical analyses. Quasi-static cyclic tests on a control column and a specimen retrofitted with a 30-mm UHPC jacket over the plastic hinge region demonstrated significant performance improvements: delayed damage initiation, controlled cracking, a 24.6% increase in lateral load capacity, 139.5% higher energy dissipation at 3% drift, and mitigated post-peak strength degradation. A validated OpenSees numerical model accurately replicated this behavior and enabled parametric studies of 15-mm, 30-mm, and 45-mm jackets. Results identified the 30-mm thickness as optimal, balancing substantial gains in lateral strength (~12% higher than other thicknesses), ductility, and energy dissipation while avoiding premature failure modes—insufficient confinement in the 15-mm jacket and strain incompatibility-induced brittle failure in the 45-mm jacket. These findings provide quantitative design guidance, establishing 30 mm as the recommended thickness for efficient seismic retrofitting of existing RC bridge columns. Full article

This study explores the development and optimization of TiO₂-based photoactive coatings enhanced with silver (Ag)—to boost photocatalytic performance—for application on glass-fiber-reinforced polyester (GFRP) and epoxy (GFRE) composites. The influence of Ag content on the structural, physicochemical, and functional properties of the coatings was evaluated. The TiO₂-Ag coating showed the best performance and was tested under UV-A irradiation and visible light (Vis), with high efficiency in VOC degradation, self-cleaning, and microbial activity. The tests were repeated in multiple runs, showing high reproducibility in the results obtained. In GFRP, pollutant and microorganism removal ratios of more than 90% were observed. In contrast, GFRE showed a lower adhesion and stability of the coating. This result is attributed to incompatibility problems with the epoxy matrix, which significantly limited its functional performance. The results highlight the feasibility of using the TiO₂-Ag coating on GFRP substrates, even under visible light. Under real-world conditions for 351 days, the coating on GFRP maintained its stability. This type of material has high potential for application in modular building systems using sandwich panels, as well as in facades and automotive components, where self-cleaning and contaminant-control properties are essential. Full article

Microplastic (MP) pollution is an escalating global environmental concern, with a growing body of research addressing diverse dimensions of this issue. Despite this progress, the field remains hindered by generating large, heterogeneous datasets that follow inconsistent reporting standards, resulting in fragmented and often incompatible databases. While various databases on MPs have been developed, they primarily operate in isolation, limiting the accessibility and cross-comparison of data. This study presents a foundational approach to aggregating and accessing existing MP pollution datasets. A comprehensive review of the currently available databases was conducted to evaluate their integration potential. It revealed key challenges such as non-standardized data formats, limited accessibility, and difficulty performing comparative analyses across sources. To address these barriers, a prototype web-based platform was developed that enables unified access to MP datasets. The architecture includes a smart standardization layer that harmonizes inputs from disparate sources. The integration of Large Language Models (LLMs) with Retrieval-Augmented Generation (RAG) techniques was proposed to facilitate natural language querying. This enables researchers to interact with the platform intuitively and extract meaningful insights more efficiently. The proposed system aims to enhance data discoverability, promote interoperability, and support robust, data-driven environmental research, paving the way toward more informed policy-making and scientific collaboration in the fight against MP pollution. With this platform, there is a potential for new discoveries and a future in which the tools to effectively combat this global issue are available, making the audience realize the potential for new discoveries. Full article

Personal lifestyle choices contribute up to 75% of national emissions and yet the greenhouse gas (GHG) inventories included in the National Inventory Report (NIR) of Canada provide limited insight on these choices. Better insight can be found using carbon footprint calculators that estimate individual emissions; however, they vary in regard to their input parameters, output data, and calculation methods. This study assessed five calculators, which are popular with the public, or compatibility with the Canadian NIR. A quantitative scoring matrix was developed to assess the output depth, academic proficiency, and effectiveness of the calculators to inform lifestyle changes, alongside NIR alignment. The results showed that the calculator with the overall highest cumulative score across all the comparative criteria was the one offered by Carbon Footprint Ltd. The other calculators that scored highly include CoolClimate Calculator and Carbon Independent. The potential of the calculators in regard to informing low-carbon lifestyles can be improved through the incorporation of more depth in terms of capturing the purchase information of goods and services and providing detailed secondary information to users, including mitigation strategies and carbon offset options. The main driver of incompatibility between the calculator tools and the NIR was the different approaches taken to the emissions inventory, with the NIR using a territorial framework and the calculators being consumption driven. The outcomes of this study demonstrate a global need for the evolution of NIR structuring to increase its relatability with citizens and for the improved standardization of publicly available tools. Full article

Cherry laurel (Prunus laurocerasus) is an understudied, highly polyploid (22×) species that is widely used as an ornamental shrub and as a fruit-bearing plant in Türkiye. We analyzed 43 accessions—33 ornamental cultivars and 10 fruit-bearing selections—by examining size variations in 10 simple sequence repeat (SSR) markers and the first intron region of the self-incompatibility ribonuclease (S-RNase) gene. A total of 498 alleles were detected across 11 loci, with the highest number of alleles observed at the S-locus. The SSR loci amplified between 4 (ASSR63) and 17 (BPPCT039) alleles per accession, with eight of the 11 primers generating more than 12 alleles per accession. Two markers, BPPCT040 and CPSCT021, uniquely distinguished all tested accessions. Of the alleles, only 178 (36%) were shared between the ornamental and fruit-bearing groups, reflecting significant genetic differentiation. A dendrogram and principal coordinate analysis revealed three distinct groups. Group 1 included most Hungarian and some European cultivars. Groups 2 (Western European cultivars) and 3 (Turkish selections) exhibited higher average allele numbers, suggesting greater genetic diversity in these groups. Our results indicate that cultivated cherry laurels originate from a broad genetic base and show clear genetic divergence between ornamental and fruit-bearing selections, likely due to differing long-term selection pressures. The observed genetic variability is consistent with the polyploid nature of the species and supports the presumed self-incompatible phenotype. This is the first study to report SSR fingerprints for ornamental cultivars and fruit-bearing selections, providing a potential tool for use in breeding programs. Full article

A combination of high potential productivity and ecological stability is essential for current cultivars, which is achievable by breeding. Interspecific/intergeneric hybridization remains a key approach to producing new high-yielding and resistant cultivars. Interspecific reproductive barriers (IRBs) appear in the interaction between the pollen and pistil of interspecific/intergeneric hybrids. The mechanisms underlying these hybridization barriers are to a considerable degree unknown. The pollen–pistil interaction is decisive because the pollen of distantly related plant species either is not recognized by stigma cells or is recognized as foreign, preventing pollen tube (PT) germination and/or penetration into the stigma/style/ovary. This review mainly focuses on (1) the pollen–pistil system; (2) IRB classification; (3) similarity and differences in the function of self-incompatibility (SI) barriers and IRBs; and (4) physiological and biochemical control of IRBs and their overcoming. The main goal is to illuminate the physiological, biochemical, and molecular mechanisms underlying the growth arrest of incompatible PTs and their death. In general, this review consolidates the current understanding of the interaction of the male gametophyte with the sporophyte tissues of the pistil and outlines future research directions in the area of plant reproductive biology. Full article

Conventional trenchless pipeline rehabilitation technologies are primarily designed for circular pipelines, with limited applicability to box culvert structures. Even when adapted, these methods often lead to significant reductions in the effective cross-sectional area and fail to enhance the structural load-bearing capacity due to geometric incompatibilities. To overcome these limitations, this study proposes a novel construction approach that employs prefabricated high-strength thin concrete segment liners for the reinforcement of underground box culverts. The feasibility of this method was validated through full-scale (1:1) experimental construction in a purpose-built test culvert, demonstrating rapid and efficient installation. A static stacking load test was subsequently conducted on the reinforced upper section of the culvert. Results indicate that the proposed reinforcement method effectively restores structural integrity and satisfies load-bearing and serviceability requirements, even after removal of the original roof slab. Additionally, a finite element analysis was performed to simulate the stacking load test conditions. The simulation revealed that variations in the mechanical properties of the grout between the existing structure and the new lining had minimal impact on the internal force distribution and deformation behavior of the prefabricated segments. The top segment consistently exhibited semi-rigid fixation behavior. This study offers a promising strategy for the rehabilitation of urban underground box culverts, achieving structural performance recovery while minimizing traffic disruption and enhancing construction efficiency. Full article