Blooming time is an important basis for constructing plant landscapes. The short flowering period of
Osmanthus fragrans, recognized as one of the ten traditional flowers in China, considerably constrains the further utilization of its resources. To clarify
O. fragrans flowering regulation, this
[...] Read more.
Blooming time is an important basis for constructing plant landscapes. The short flowering period of
Osmanthus fragrans, recognized as one of the ten traditional flowers in China, considerably constrains the further utilization of its resources. To clarify
O. fragrans flowering regulation, this study focused on
OfCOR27, conducting cloning, expression analysis, and functional verification to explore its effects on
O. fragrans flowering time. A
COR27 phylogenetic tree was built across six species;
OfCOR27 physicochemical properties, conserved structures, and promoter cis-elements were analyzed.
OfCOR27 CDS was cloned, fusion vectors were transformed into
Nicotiana benthamiana, and organ-specific expression was tested in two
O. fragrans cultivars. Overexpression vectors were transformed into
Arabidopsis thaliana, with qRT-PCR verifying gene function. Five
OfCOR27s were identified, showing evolutionary conservation.
OfCOR27, which localizes to the nucleus and is associated with flowering regulation, shows higher expression in ‘Sijigui’ than in ‘XiaoyeSugui’. Overexpression of
OfCOR27 promoted flowering in
A. thaliana, whereas the
AtCOR27 mutant flowered later. This confirms
OfCOR27 is a positive regulator of plant flowering, which may promote flowering by enhancing the expression of flowering-promoting genes and altering hormone levels, providing a theoretical basis and candidate gene for the genetic improvement of flowering traits in woody ornamental plants.
Full article
