Search Results (8,373)

Accurate prediction of key ocean elements (e.g., chlorophyll-a concentration, sea surface temperature, etc.) is imperative for maintaining marine ecological balance, responding to marine disaster pollution, and promoting the sustainable use of marine resources. Existing spatio-temporal prediction models primarily rely on either physical or data-driven approaches. Physical models are constrained by modeling complexity and parameterization errors, while data-driven models lack interpretability and depend on high-quality data. To address these challenges, this study proposes OKG-ConvGRU, a domain knowledge-guided remote sensing prediction framework for ocean elements. This framework integrates knowledge graphs with the ConvGRU network, leveraging prior knowledge from marine science to enhance the prediction performance of ocean elements in remotely sensed images. Firstly, we construct a spatio-temporal knowledge graph for ocean elements (OKG), followed by semantic embedding representation for its spatial and temporal dimensions. Subsequently, a cross-attention-based feature fusion module (CAFM) is designed to efficiently integrate spatio-temporal multimodal features. Finally, these fused features are incorporated into an enhanced ConvGRU network. For multi-step prediction, we adopt a Seq2Seq architecture combined with a multi-step rolling strategy. Prediction experiments for chlorophyll-a concentration in the eastern seas of China validate the effectiveness of the proposed framework. The results show that, compared to baseline models, OKG-ConvGRU exhibits significant advantages in prediction accuracy, long-term stability, data utilization efficiency, and robustness. This study provides a scientific foundation and technical support for the precise monitoring and sustainable development of marine ecological environments. Full article

Objectives: This study investigates whether scan time in the high-resolution magnetic resonance imaging (MRI) of human embryos can be reduced without compromising spatial resolution by applying zero-shot self-supervised learning (ZS-SSL), a deep-learning-based reconstruction method. Methods: Simulations using a numerical phantom were conducted to evaluate spatial resolution across various acceleration factors (AF = 2, 4, 6, and 8) and signal-to-noise ratio (SNR) levels. Resolution was quantified using a blur-based estimation method based on the Sparrow criterion. ZS-SSL was compared to conventional compressed sensing (CS). Experimental imaging of a human embryo at Carnegie stage 21 was performed at a spatial resolution of (30 μm)³ using both retrospective and prospective undersampling at AF = 4 and 8. Results: ZS-SSL preserved spatial resolution more effectively than CS at low SNRs. At AF = 4, image quality was comparable to that of fully sampled data, while noticeable degradation occurred at AF = 8. Experimental validation confirmed these findings, with clear visualization of anatomical structures—such as the accessory nerve—at AF = 4; there was reduced structural clarity at AF = 8. Conclusions: ZS-SSL enables significant scan time reduction in high-resolution MRI of human embryos while maintaining spatial resolution at AF = 4, assuming an SNR above approximately 15. This trade-off between acceleration and image quality is particularly beneficial in studies with limited imaging time or specimen availability. The method facilitates the efficient acquisition of ultra-high-resolution data and supports future efforts to construct detailed developmental atlases. Full article

How do humans analyze the sentiments embedded in text? When attempting to analyze a text, humans construct a “scene” in their minds through imagination based on the text, generating a vague image. They then synthesize the text and the mental image to derive the final analysis result. However, current sentiment analysis models lack such imagination; they can only analyze based on existing information in the text, which limits their classification accuracy. To address this issue, we propose the SceEmoNet model. This model endows text classification models with imagination through Stable diffusion, enabling the model to generate corresponding visual scenes from input text, thus introducing a new modality of visual information. We then use the Contrastive Language-Image Pre-training (CLIP) model, a multimodal feature extraction model, to extract aligned features from different modalities, preventing significant feature differences caused by data heterogeneity. Finally, we fuse information from different modalities using late fusion to obtain the final classification result. Experiments on six datasets with different classification tasks show improvements of 9.57%, 3.87%, 3.63%, 3.14%, 0.77%, and 0.28%, respectively. Additionally, we set up experiments to deeply analyze the model’s advantages and limitations, providing a new technical path for follow-up research. Full article

To address the inefficiency of traditional concrete crack detection methods and the heavy reliance of supervised learning on extensive labeled data, in this study, an intelligent assessment method of concrete damage based on pseudo-label semi-supervised learning and fractal geometry theory is proposed to solve two core tasks: one is binary classification of pixel-level cracks, and the other is multi-category assessment of damage state based on crack morphology. Using three-channel RGB images as input, a dual-path collaborative training framework based on U-Net encoder–decoder architecture is constructed, and a binary segmentation mask of the same size is output to achieve the accurate segmentation of cracks at the pixel level. By constructing a dual-path collaborative training framework and employing a dynamic pseudo-label refinement mechanism, the model achieves an F1-score of 0.883 using only 50% labeled data—a mere 1.3% decrease compared to the fully supervised benchmark DeepCrack (F1 = 0.896)—while reducing manual annotation costs by over 60%. Furthermore, a quantitative correlation model between crack fractal characteristics and structural damage severity is established by combining a U-Net segmentation network with the differential box-counting algorithm. The experimental results demonstrate that under a cyclic loading of 147.6–221.4 kN, the fractal dimension monotonically increases from 1.073 (moderate damage) to 1.189 (failure), with 100% accuracy in damage state identification, closely aligning with the degradation trend of macroscopic mechanical properties. In complex crack scenarios, the model attains a recall rate (Re = 0.882), surpassing U-Net by 13.9%, with significantly enhanced edge reconstruction precision. Compared with the mainstream models, this method effectively alleviates the problem of data annotation dependence through a semi-supervised strategy while maintaining high accuracy. It provides an efficient structural health monitoring solution for engineering practice, which is of great value to promote the application of intelligent detection technology in infrastructure operation and maintenance. Full article

This study presents a cross-cultural comparison of two fortified residences—Palazzo Farnese in Italy and Dong’s Fortified Compound in China—through a triadic analytical framework encompassing architectural form, symbolic ornamentation, and public perception. By combining field observation, iconographic interpretation, and digital ethnography, the research investigates how heritage meaning is constructed, encoded, and reinterpreted across distinct sociocultural contexts. Empirical materials include architectural documentation, decorative analysis, and a curated dataset of 4947 user-generated images and 1467 textual comments collected from Chinese and international platforms between 2020 and 2024. Methods such as CLIP-based visual clustering and BERTopic-enabled sentiment modelling were applied to extract patterns of perception and symbolic emphasis. The findings reveal contrasting representational logics: Palazzo Farnese encodes dynastic authority and Renaissance cosmology through geometric order and immersive frescoes, while Dong’s Compound conveys Confucian ethics and frontier identity via nested courtyards and traditional ornamentation. Digital responses diverge accordingly: international users highlight formal aesthetics and photogenic elements; Chinese users engage with symbolic motifs, family memory, and ritual significance. This study illustrates how historically fortified residences are reinterpreted through culturally specific digital practices, offering an interdisciplinary approach that bridges architectural history, symbolic analysis, and digital heritage studies. Full article

The unique characteristics of desert vegetation, such as different leaf morphology, discrete canopy structures, sparse and uneven distribution, etc., pose significant challenges for remote sensing-based estimation of fractional vegetation cover (FVC). The Unmanned Aerial Vehicle (UAV) system can accurately distinguish vegetation patches, extract weak vegetation signals, and navigate through complex terrain, making it suitable for applications in small-scale FVC extraction. In this study, we selected the floodplain fan with Caragana korshinskii Kom as the constructive species in Hatengtaohai National Nature Reserve, Bayannur, Inner Mongolia, China, as our study area. We investigated the remote sensing extraction method of desert sparse vegetation cover by placing samples across three gradients: the top, middle, and edge of the fan. We then acquired UAV multispectral images; evaluated the applicability of various vegetation indices (VIs) using methods such as supervised classification, linear regression models, and machine learning; and explored the feasibility and stability of multiple machine learning models in this region. Our results indicate the following: (1) We discovered that the multispectral vegetation index is superior to the visible vegetation index and more suitable for FVC extraction in vegetation-sparse desert regions. (2) By comparing five machine learning regression models, it was found that the XGBoost and KNN models exhibited relatively lower estimation performance in the study area. The spatial distribution of plots appeared to influence the stability of the SVM model when estimating fractional vegetation cover (FVC). In contrast, the RF and LASSO models demonstrated robust stability across both training and testing datasets. Notably, the RF model achieved the best inversion performance (R² = 0.876, RMSE = 0.020, MAE = 0.016), indicating that RF is one of the most suitable models for retrieving FVC in naturally sparse desert vegetation. This study provides a valuable contribution to the limited existing research on remote sensing-based estimation of FVC and characterization of spatial heterogeneity in small-scale desert sparse vegetation ecosystems dominated by a single species. Full article

Our understanding of foreland basin subsurface structures relies heavily on seismic reflection data. The seismic profile across the eastern Rioni foreland basin in western Georgia is critical for characterizing its deformation structural style. We applied fault-related folding and thrust wedge theories to interpret the seismic profile and construction structural cross-section, which reveals that compressional structures are controlled by multiple detachment levels. Both thin-skinned and thick-skinned structures are identified. The seismic profile and structural cross-section reveal the presence of normal faults, reverse faults, thrust faults, duplexes, triangle zone, and crustal-scale duplexes. The deep-level detachment within the basement is responsible for the development of the crustal-scale duplexes. These structures appear to have formed through the reactivation of pre-existing normal faults during compressive deformation. Based on our interpretation, the imaged duplex system likely represents the western subsurface continuation of the Dzirula Massif. Full article

Tissue-engineered scaffolds, particularly composite scaffolds composed of polymers combined with ceramics, bioactive glasses, or nanomaterials, play a vital role in regenerative medicine by providing structural and biological support for tissue repair. As scaffold designs grow increasingly complex, the need for non-invasive imaging modalities capable of monitoring scaffold integration, degradation, and tissue regeneration in real-time has become critical. This review summarizes current non-invasive imaging techniques used to evaluate tissue-engineered constructs, including optical methods such as near-infrared fluorescence imaging (NIR), optical coherence tomography (OCT), and photoacoustic imaging (PAI); magnetic resonance imaging (MRI); X-ray-based approaches like computed tomography (CT); and ultrasound-based modalities. It discusses the unique advantages and limitations of each modality. Finally, the review identifies major challenges—including limited imaging depth, resolution trade-offs, and regulatory hurdles—and proposes future directions to enhance translational readiness and clinical adoption of imaging-guided tissue engineering (TE). Emerging prospects such as multimodal platforms and artificial intelligence (AI) assisted image analysis hold promise for improving precision, scalability, and clinical relevance in scaffold monitoring. Full article

Accurate detection of maize tassels plays a crucial role in yield estimation of maize in precision agriculture. Recently, UAV and deep learning technologies have been widely introduced in various applications of field monitoring. However, complex field backgrounds pose multiple challenges against the precision detection of maize tassels, including maize tassel multi-scale variations caused by varietal differences and growth stage variations, intra-class occlusion, and background interference. To achieve accurate maize tassel detection in UAV images under complex field backgrounds, this study proposes an MSMT-RTDETR detection model. The Faster-RPE Block is first designed to enhance multi-scale feature extraction while reducing model Params and FLOPs. To improve detection performance for multi-scale targets in complex field backgrounds, a Dynamic Cross-Scale Feature Fusion Module (Dy-CCFM) is constructed by upgrading the CCFM through dynamic sampling strategies and multi-branch architecture. Furthermore, the MPCC3 module is built via re-parameterization methods, and further strengthens cross-channel information extraction capability and model stability to deal with intra-class occlusion. Experimental results on the MTDC-UAV dataset demonstrate that the MSMT-RTDETR significantly outperforms the baseline in detecting maize tassels under complex field backgrounds, where a precision of 84.2% was achieved. Compared with Deformable DETR and YOLOv10m, improvements of 2.8% and 2.0% were achieved, respectively, in the mAP₅₀ for UAV images. This study proposes an innovative solution for accurate maize tassel detection, establishing a reliable technical foundation for maize yield estimation. Full article

Diacylglycerol-O-acyltransferase 1 (DGAT1, EC 2.3.1.20) is a pivotal enzyme in plant triacylglycerol (TAG) biosynthesis. Previous work identified conserved di-arginine (R) motifs (R-R, R-X-R, and R-X-X-R) in its N-terminal cytoplasmic acyl-CoA binding domain. To elucidate their functional significance, we engineered R-rich sequences in the N-termini of Tropaeolum majus and Zea mays DGAT1s. Comparative analysis with their respective non-mutant constructs showed that deleting or substituting R with glycine in the N-terminal region of DGAT1 markedly reduced lipid accumulation in both Camelina sativa seeds and Saccharomyces cerevisiae cells. Immunofluorescence imaging revealed co-localization of non-mutant and R-substituted DGAT1 with lipid droplets (LDs). However, disruption of an N-terminal di-R motif destabilizes DGAT1, alters LD organization, and impairs recombinant oleosin retention on LDs. Further evidence suggests that the di-R motif mediates DGAT1 retrieval from LDs to the endoplasmic reticulum (ER), implicating its role in dynamic LD–ER protein trafficking. These findings establish the conserved di-R motifs as important regulators of DGAT1 function and LD dynamics, offering insights for the engineering of oil content in diverse biological systems. Full article

High moisture content (MC) harms wheat storage quality and readily leads to mold growth. Accurate localization of abnormal/high-moisture regions enables early warning, ensuring proper storage and reducing economic losses. The present study introduces the 2D microwave scanning method and investigates a novel localization method for addressing such a challenge. Both static and scanning experiments were performed on a developed mobile and non-destructive microwave detection system to quantify the MC of wheat and then locate abnormal moisture regions. For quantifying the wheat’s MC, a dual-parameter wheat MC prediction model with the random forest (RF) algorithm was constructed, achieving a high accuracy (R² = 0.9846, MSE = 0.2768, MAE = 0.3986). MC scanning experiments were conducted by synchronized moving waveguides; the maximum absolute error of MC prediction was 0.565%, with a maximum relative error of 3.166%. Furthermore, both one- and two-dimensional localizing methods were proposed for localizing abnormal moisture regions. The one-dimensional method evaluated two approaches—attenuation value and absolute attenuation gradient—using computer simulation technology (CST) modeling and scanning experiments. The experimental results confirmed the superior performance of the absolute gradient method, with a center detection error of less than 12 mm in the anomalous wheat moisture region and a minimum width detection error of 1.4 mm. The study performed two-dimensional antenna scanning and effectively imaged the high-MC regions using phase delay analysis. The imaging results coincide with the actual locations of moisture anomaly regions. This study demonstrated a promising solution for accurately localizing the wheat’s abnormal/high-moisture regions with the use of an emerging microwave transmission method. Full article

The transition to efficient production requires innovative water control techniques to maximize irrigation efficiency and minimize waste. Analyzing and optimizing irrigation practices is essential to improve water use and reduce environmental impact. The aim of the research was to identify a discrimination method to analyze the different hydration levels in baby-leaf products. The species being researched was spinach, harvested at the baby leaf stage. Utilizing a large dataset of 261 wavelengths from the hyperspectral imaging system, the feature selection minimum redundancy maximum relevance (FS-MRMR) algorithm was applied, leading to the development of a neural network-based prediction model. Finally, a mathematical classification model K-NN (k-nearest neighbors type) was developed in order to identify a transfer function capable of discriminating the hyperspectral data based on a threshold value of absolute leaf humidity. Five significant wavelengths were identified for estimating the moisture content of baby leaves. The resulting model demonstrated a high generalization capability and excellent correlation between predicted and measured data, further confirmed by the successful training, validation, and testing of a K-NN-based statistical classifier. The construction phase of the statistical classifier involved the use of the experimental dataset and the critical humidity threshold value of 0.83 (83% of leaf humidity) was considered, below which the baby-leaf crop requires the irrigation intervention. High percentages of correct classification were achieved for data within two humidity classes. Specifically, the statistical classifier demonstrated excellent performance, with 81.3% correct classification for samples below the threshold and 99.4% for those above it. The application of advanced spectral analysis and artificial intelligence methods has led to significant progress in leaf moisture analysis and prediction, yielding substantial implications for both agriculture and biological research. Full article

Objectives: Automatic tip localization is critical in ultrasound (US)-guided interventions. Although deep learning (DL) has been widely used for precise tip detection, existing methods are limited by the availability of real puncture data and expert annotations. Methods: To address these challenges, we propose a novel method that uses synthetic US puncture data to pre-train DL-based tip detectors, improving their generalization. Synthetic data are generated by fusing clinical US images of healthy controls with tips created using generative DL models. To ensure clinical diversity, we constructed a dataset from scans of 20 volunteers, covering 20 organs or anatomical regions, obtained with six different US machines and performed by three physicians with varying expertise levels. Tip diversity is introduced by generating a wide range of synthetic tips using a denoising probabilistic diffusion model (DDPM). This method synthesizes a large volume of diverse US puncture data, which are used to pre-train tip detectors, followed by subsequently training with real puncture data. Results: Our method outperforms MSCOCO pre-training on a clinical puncture dataset, achieving a 1.27–7.19% improvement in AP_0.1:0.5 with varying numbers of real samples. State-of-the-art detectors also show performance gains of 1.14–1.76% when applying the proposed method. Conclusions: The experimental results demonstrate that our method enhances the generalization of tip detectors without relying on expert annotations or large amounts of real data, offering significant potential for more accurate visual guidance during US-guided interventions and broader clinical applications. Full article

Focusing on the problem of insufficient detection accuracy caused by blurred target boundaries, variable scales, and severe noise interference in side-scan sonar images, this paper proposes a high-precision detection network named ESL-YOLO, which integrates edge perception and adaptive quality assessment. Firstly, an Edge Fusion Module (EFM) is designed, which integrates the Sobel operator into depthwise separable convolution. Through a dual-branch structure, it realizes effective fusion of edge features and spatial features, significantly enhancing the ability to recognize targets with blurred boundaries. Secondly, a Self-Calibrated Dual Attention (SCDA) Module is constructed. By means of feature cross-calibration and multi-scale channel attention fusion mechanisms, it achieves adaptive fusion of shallow details and deep-rooted semantic content, improving the detection accuracy for small-sized targets and targets with elaborate shapes. Finally, a Location Quality Estimator (LQE) is introduced, which quantifies localization quality using the statistical characteristics of bounding box distribution, effectively reducing false detections and missed detections. Experiments on the SIMD dataset show that the mAP@0.5 of ESL-YOLO reaches 84.65%. The precision and recall rate reach 87.67% and 75.63%, respectively. Generalization experiments on additional sonar datasets further validate the effectiveness of the proposed method across different data distributions and target types, providing an effective technical solution for side-scan sonar image target detection. Full article

Background: During infection with the cytomegalovirus (CMV), the membrane system of the infected cell is remodelled into a megastructure called the assembly compartment (AC). These extensive changes may involve the manipulation of the host cell proteome by targeting a pleiotropic function of the cell such as ubiquitination (Ub). In this study, we investigate whether the Ub system is required for the establishment and maintenance of the AC in murine CMV (MCMV)-infected cells Methods: NIH3T3 cells were infected with wild-type and recombinant MCMVs and the Ub system was inhibited with PYR-41. The expression of viral and host cell proteins was analyzed by Western blot. AC formation was monitored by immunofluorescence with confocal imaging and long-term live imaging as the dislocation of the Golgi and expansion of Rab10-positive tubular membranes (Rab10 TMs). A cell line with inducible expression of hemagglutinin (HA)-Ub was constructed to monitor ubiquitination. siRNA was used to deplete host cell factors. Infectious virion production was monitored using the plaque assay. Results: The Ub system is required for the establishment of the infection, progression of the replication cycle, viral gene expression and production of infectious virions. The Ub system also regulates the establishment and maintenance of the AC, including the expansion of Rab10 TMs. Increased ubiquitination of WASHC1, which is recruited to the machinery that drives the growth of Rab10 TMs, is consistent with Ub-dependent rheostatic control of membrane tubulation and the continued expansion of Rab10 TMs. Conclusions: The Ub system is intensively utilized at all stages of the MCMV replication cycle, including the reorganization of the membrane system into the AC. Disruption of rheostatic control of the membrane tubulation by ubiquitination and expansion of Rab10 TREs within the AC may contribute to the development of a sufficient amount of tubular membranes for virion envelopment. Full article