Search Results (134)

Water is vital for producing summer maize (SM) and winter wheat (WW); therefore, its proper management is crucial for sustainable farming. This study aimed to develop new tri-band spectral vegetation indices that enhance the accuracy of monitoring plant moisture content (PMC) in SM and WW. We conducted irrigation treatments, including W0, W1, W2, W3, and W4, in SM–WW rotations to address this issue. Canopy reflectance was measured with a field spectroradiometer. Tri-band hyperspectral vegetation indices were constructed: Normalised Water Stress Index (NWSI), Normalised Difference Index (NDI), and Exponential Water Stress Index (EWSI), for assessing the PMC of SM and WW. Results indicate that NWSI outperformed other indices. In the maize trials, the correlation reached R = −0.8369, while in wheat, it reached R = −0.9313, surpassing traditional indices. Four mainstream machine learning models (Random Forest, Partial Least Squares Regression, Support Vector Machine, and Artificial Neural Network) were employed for modelling. NWSI-PLSR exhibited the best index-type performance with an R² of 0.7878. When the new indices were combined with traditional indices as input data, the NWSI-Published indices-SVM model achieved superior performance with an R² of 0.8203, outperforming other models. The RF model produced the most consistent performance and achieved the highest average R² across all input types. The NDI-Published indices models also outperformed those of the published indices alone. This indicates that these new indices improve the accuracy of moisture content monitoring in SM and WW fields. It provides a technical basis and support for precision irrigation, holding significant potential for application. Full article

Accurate prediction of photosynthetic parameters is pivotal for precision viticulture, as it enables non-invasive monitoring of plant physiological status and informed management decisions. In this study, spectral reflectance data were used to predict key photosynthetic parameters such as assimilation rate (A), effective photosystem II (PSII) quantum yield (${\Phi }_{\mathrm{PSII}}$), and electron transport rate (ETR), as well as stem and leaf water potential (${\Psi }_{\mathrm{stem}}$ and ${\Psi }_{\mathrm{leaf}}$), in Vitis vinifera (cv. Müller-Thurgau) grown in an experimental vineyard in Lower Franconia (Germany). Measurements were obtained on 25 July, 7 August, and 12 August 2024 using a LI-COR LI-6800 system and a PSR+ hyperspectral spectroradiometer. Various machine learning models (SVR, Lasso, ElasticNet, Ridge, PLSR, a simple ANN, and Random Forest) were evaluated, both as standalone predictors and as base learners in a stacking ensemble regressor with a Random Forest meta-learner. First derivative reflectance (FDR) preprocessing enhanced predictive performance, particularly for ${\Phi }_{\mathrm{PSII}}$ and ETR, with the ensemble approach achieving R² values up to 0.92 for ${\Phi }_{\mathrm{PSII}}$ and 0.85 for A at 1 nm resolution. At coarser spectral resolutions, predictive accuracy declined, though FDR preprocessing provided some mitigation of the performance loss. Diurnal patterns revealed that morning to mid-morning measurements, particularly between 9:00 and 11:00, captured peak photosynthetic activity, making them optimal for assessing vine vigor, while midday water potential declines indicated favorable timing for irrigation scheduling. These findings demonstrate the potential of integrating hyperspectral data with ensemble machine learning and FDR preprocessing for accurate, scalable, and high-throughput monitoring of grapevine physiology, supporting real-time vineyard management and the use of cost-effective sensors under diverse environmental conditions. Full article

The cereal leaf beetle (CLB; Oulema melanopus L., Coleoptera: Chrysomelidae) is a serious pest of wheat, capable of causing yield losses of up to 40% through photosynthetic impairment. Early detection and severity assessment are essential for effective and sustainable pest management. This study evaluates the potential of hyperspectral remote sensing (RS) combined with machine learning (ML) for non-invasive detection of CLB-induced stress in winter wheat. Spectral reflectance was measured using a full-range spectroradiometer (350–2500 nm) from flag leaves categorized into four damage levels (healthy, slightly, moderately, and severely damaged). Three input datasets were used for ML classification: full spectral reflectance, a set of 13 vegetation indices (VIs), and outputs of dimensionality reduction technique. CLB stress increased reflectance in the visible range (400–700 nm) and reduced it in the near-infrared (700–1400 nm), consistent with chlorophyll degradation and mesophyll damage. Several VIs, including RIGreen, NDVI750, GNDVI, and NDVI, correlated strongly with damage severity (τ = 0.78–0.81). Among the six ML models tested, Support Vector Machine (SVM) achieved the highest classification accuracy of 90.0% (precision = 0.90, recall = 0.90, F1 = 0.90) across the four severity classes, and achieved 91.9% accuracy at the early-detection threshold. As far as the currently available literature indicates, this study provides one of the earliest quantitative assessments of CLB damage severity based on full-spectrum leaf-level hyperspectral reflectance integrated with ML classification. These findings were obtained under controlled, leaf-level measurement conditions and therefore represent a proof-of-concept; future validation using UAV and satellite platforms is needed to assess performance under operational field variability. Overall, our findings highlight the potential of hyperspectral RS and ML for precision pest monitoring, supporting threshold-based decision-making and more sustainable insecticide use. Full article

Rising global temperatures pose a significant threat to agricultural productivity, making the early detection of plant stress crucial for minimizing crop losses. While hyperspectral remote sensing is a powerful tool for monitoring plant health, the precise spectral regions and most effective machine learning models for detecting thermal stress remain an open research question. This study presents a robust framework that utilizes eight state-of-the-art machine learning algorithms to classify the reflectance response of thermal-induced stress in two cultivars of bean plants. Our controlled experiment measured hyperspectral data across two growth stages and three stress conditions (pre-stress, during stress, and post-stress) using a spectroradiometer. The results demonstrate the high performance of several algorithms, with the Artificial Neural Network (ANN) achieving an impressive 99.4% overall accuracy. A key contribution of this work is the identification of the most contributory spectral ranges for thermal stress discrimination: the green region (530–570 nm) and the red-edge region (700–710 nm). This framework is a feasible and effective tool for modelling the hyperspectral response of thermal-stressed bean plants and provides critical guidance for future research on stress-specific spectral indices. Full article

Agroforestry systems offer clear environmental and agronomic advantages, but their effect on plant–biotic stressor interactions remains poorly understood. Specifically, the shade from companion trees can create microclimates favorable to fungal diseases on herbaceous crops. This potential drawback may offset other benefits, highlighting the urgent need for advanced plant health monitoring in these systems. This study assessed the potential of hyperspectral reflectance to detect the single and combined effects of simulated tree shading and infection by the fungal pathogen Alternaria alternata on grain sorghum (Sorghum bicolor L. Moench) under rainfed field conditions. Sorghum was grown either under full light or 50% shading conditions. Half of the plots were artificially inoculated with an A. alternata spore suspension (2 × 10⁸ CFU mL⁻¹), while the others served as controls. Leaf and ground-canopy measurements were acquired with a full range spectroradiometer (VNIR-SWIR, 400–2,400 nm) and UAV imagery covered the VIS-NIR range (400–1,000 nm) before the onset of visible symptoms. Permutational multivariate analysis of variance of leaf and ground-canopy data revealed significant effects of shading (Sh), infection (Aa), and their interaction (p < 0.05), allowing early detection of infection two days before symptom appearance, while UAV data showed only singular significant effects. Partial least squares discriminant analysis accuracy reached 78% at the leaf level, 90% at the ground-canopy level, and 74% (Sh) and 75% (Aa) at the UAV scale. Furthermore, vegetation spectral indices derived from the spectra confirmed greater physiological stress in shaded and infected plants, consistent with disease incidence assessments. Our results establish scale-specific hyperspectral reflectance spectroscopy as a powerful, non-destructive technique for early plant health surveillance in agroforestry. This advanced optical sensing capability is poised to illuminate complex stressor interactions, marking a significant step forward for precision agroforestry management. Full article

Accurate and timely assessment of crop disease severity is crucial for effective management strategies and ensuring sustainable agricultural production. Traditional visual disease scoring methods are subjective and labor-intensive, highlighting the need for automated, objective alternatives. This study evaluates the effectiveness of a model for field-based identification and quantification of stripe rust severity in wheat using red, green, blue RGB imaging. Based on crop reflectance hyperspectra (CRHS) acquired using a FieldSpec ASD spectroradiometer, two complementary approaches were developed. In the first approach, we estimate single leaf disease severity (LDS) under laboratory conditions, while in the second approach, we assess crop disease severity (CDS) from field-based RGB images. The high accuracy of both methods enabled the development of a predictive model for estimating LDS from CDS, offering a scalable solution for precision disease monitoring in wheat cultivation. The experiment was conducted on four winter wheat plots subjected to varying fungicide treatments to induce different levels of stripe rust severity for model calibration, with treatment regimes ranging from no application to three applications during the growing season. RGB images were acquired in both laboratory conditions (individual leaves) and field conditions (nadir and oblique perspectives), complemented by hyperspectral measurements in the 350–2500 nm range. To achieve automated and objective assessment of disease severity, we developed custom image-processing scripts and applied Random Forest classification and regression models. The models demonstrated high predictive performance, with the combined use of nadir and oblique RGB imagery achieving the highest classification accuracy (97.87%), sensitivity (100%), and specificity (95.83%). Oblique images were more sensitive to early-stage infection, while nadir images offered greater specificity. Spectral feature selection revealed that wavelengths in the visible (e.g., 508–563 nm and 621–703 nm) and red-edge/SWIR regions (around 1556–1767 nm) were particularly informative for disease detection. In classification models, shorter wavelengths from the visible range proved to be more useful, while in regression models, longer wavelengths were more effective. The integration of RGB-based image analysis with the Random Forest algorithm provides a robust, scalable, and cost-effective solution for monitoring stripe rust severity under field conditions. This approach holds significant potential for enhancing precision agriculture strategies by enabling early intervention and optimized fungicide application. Full article

Spectral reflectance of plants can be readily associated with physiological and biochemical parameters. Thus, relating spectral data to amino acid contents in different genetic materials provides an innovative and efficient approach for understanding and managing genetic diversity. Therefore, this study had two objectives: (I) to differentiate genetic materials according to amino acid contents and spectral reflectance; (II) to establish the relationship between amino acids and spectral bands derived from hyperspectral data. The research was conducted with 32 soybean genetic materials grown in the field during the 2023–2024 crop year. The experimental design involved randomized blocks with four replicates. Leaf spectral data were collected 60 days after plant emergence, when the plants were in full bloom. Three leaf samples were collected from the third fully developed trifoliate leaf, counted from top to bottom, from each plot. The samples were taken to the laboratory, where reflectance readings were obtained using a spectroradiometer, which can measure the 350–2500 nm spectrum. Wavelengths were grouped as means of representative intervals and then organized into 28 bands. Subsequently, the leaf samples from each plot were subjected to quantification analyses for 17 amino acids. Then, the soybean genotypes were subjected to a PCA–K-means analysis to separate the genotypes according to their amino acid content and spectral behavior. A correlation network was constructed to investigate the relationships between the spectral variables and between the amino acids within each group. The groups formed by the different genetic materials exhibited distinct profiles in both amino acid composition and spectral behavior. Leaf reflectance data proved to be efficient in identifying differences between soybean genotypes regarding the amino acid content in the leaves. Leaf reflectance was effective in distinguishing soybean genotypes according to leaf amino acid content. Specific and high-magnitude associations were found between spectral bands and amino acids. Our findings reveal that spectral reflectance can serve as a reliable, non-destructive indicator of amino acid composition in soybean leaves, supporting advanced phenotyping and selection in breeding programs. Full article

Gold kiwifruits from two different farms, harvested at different times, were analysed using both non-destructive and destructive methods. A computer vision system (CVS) and a portable spectroradiometer were used to perform non-destructive measurements of firmness, titratable acidity, pH, soluble solids content, dry matter, and soluble sugars (glucose and fructose), with the goal of building predictive models for the maturity index. Hyperspectral data from the visible–near-infrared (VIS–NIR) and short-wave infrared (SWIR) ranges, collected via the spectroradiometer, along with colour features extracted by the CVS, were used as predictors. Three different regression methods—Partial Least Squares (PLS), Support Vector Regression (SVR), and Gaussian process regression (GPR)—were tested to assess their predictive accuracy. The results revealed a significant increase in sugar content across the different harvesting times in the season. Regardless of the regression method used, the CVS was not able to distinguish among the different harvests, since no significant skin colour changes were measured. Instead, hyperspectral measurements from the near-infrared (NIR) region and the initial part of the SWIR region proved useful in predicting soluble solids content, glucose, and fructose. The models built using these spectral regions achieved R² average values between 0.55 and 0.60. Among the different regression models, the GPR-based model showed the best performance in predicting kiwifruit soluble solids content, glucose, and fructose. In conclusion, for the first time, the effectiveness of a fully portable spectroradiometer measuring surface reflectance until the full SWIR range for the rapid, contactless, and non-destructive estimation of the maturity index of kiwifruits was reported. The versatility of the portable spectroradiometer may allow for field applications that accurately identify the most suitable moment to carry out the harvesting. Full article

Enhancing the spatial resolution of gypsiferous soil detection, as a valuable baseline information layer, is beneficial for investigating agroecological processes and tackling land degradation in semi-arid environments. This study evaluates the performance of PRISMA (PRecursore IperSpettrale della Missione Applicativa) and EnMAP (Environmental Mapping and Analysis Program) satellites in estimating soil gypsum content and compares models trained on satellite imagery versus lab data. To this end, 242 bare-soil samples were collected from southeast Iran. Gypsum content was measured using acetone precipitation, and spectral reflectance was acquired using the ASD (Analytical Spectral Devices)-Fieldspec 3 spectroradiometer. The gypsum content was retrieved by optical data using three approaches: narrowband indices, spectral absorption features, and machine learning (ML) algorithms. Four machine learning algorithms, including PLSR (Partial Least Squares Regression), RF (Random Forest), SVR (Support Vector Regression), and GPR (Gaussian Process Regression), achieved excellent performance (RPD > 2.5). The results showcased that the difference soil index (DSI) achieved the highest R² scores of 0.96 (ASD), 0.79 (PRISMA), and 0.84 (EnMAP), slightly outperforming the normalized difference gypsum ratio (NDGI) and ratio soil index (RSI). Comparing the shape indices’, the slope parameter (SLP) index outperformed the half-area parameter (HAP) index. PRISMA, with SVR (R² ≥ 0.83), and EnMAP, with PLSR (R² ≥ 0.85), demonstrated that hyperspectral satellites proved reliable in detecting gypsum content, yielding results comparable to ASD with detailed algorithms. Full article

An attempt was made to quantify soil properties using hyperspectral remote-sensing techniques and machine-learning algorithms. In total, 100 soil samples representing various locations and soil-nutrient statuses were collected, and the samples were analyzed for soil pH, EC, soil organic carbon, available nitrogen (AN), available phosphorus (AP), and available potassium (AK) by following standard methods. Soil had a wide range of properties, i.e., pH varied from 5.62 to 8.49, EC varied from 0.08 to 1.78 dS/m, soil organic carbon varied from 0.23 to 0.94%, available nitrogen varied from 154 to 344 kg/ha, available phosphorus varied from 9.5 to 25.5 kg/ha, and available potassium varied from 131 to 747 kg/ha. The same set of soil samples were subjected to spectral reflectance measurement using SVC GER 1500 Spectroradiometer (spectral range: 350 to 1050 nm). The measured spectral signatures of various soils were organized for developing a spectral library and for deriving various spectral indices to correlate with soil properties to quantify the nutrients. The soil samples were partitioned into 60:40 ratios for training and validation, respectively. In order to select optimum bands (wavelength) from the soil spectra, we have employed metaheuristic algorithms i.e., Particle Swarm Optimization (PSO), Moth–Flame optimization (MFO), Flower Pollination Optimization (FPO), and Battle Royale Optimization (BRO) algorithm. Further partial least square regression (PLSR) was used to find the latent variable and to evaluate various algorithms for their performance in predicting soil properties. The results indicated that nutrients could be quantified from spectral reflectance measurement with fair to good accuracy through the Battle Royale Optimization technique with a $R^2$ value of 0.45, 0.32, 0.48, 0.21, 0.71, and 0.35 for pH, EC, soil organic carbon, available-N, available-P, and available-K, respectively. Full article

Remote sensing (RS) has been widely used to monitor cyanobacterial blooms in inland water bodies. However, the accuracy of RS-based monitoring varies significantly depending on factors such as waterbody type, sensor characteristics, and analytical methods. This study comprehensively evaluates the current capabilities and challenges of RS for cyanobacterial bloom monitoring, with a focus on achievable accuracy. We find that chlorophyll-a (Chl-a) and phycocyanin (PC) are the primary indicators used, with PC demonstrating greater accuracy and stability than Chl-a. Sentinel and Landsat satellites are the most frequently used RS data sources, while hyperspectral images, particularly from unmanned aerial vehicles (UAVs), have shown high accuracy in recent years. In contrast, the Medium-Resolution Imaging Spectrometer (MERIS) and Moderate-Resolution Imaging Spectroradiometer (MODIS) have exhibited lower performance. The choice of analytical methods is also essential for monitoring accuracy, with regression and machine learning models generally outperforming other approaches. Temporal analysis indicates a notable improvement in monitoring accuracy from 2021 to 2023, reflecting advances in RS technology and analytical techniques. Additionally, the findings suggest that a combined approach using Chl-a for large-scale preliminary screening, followed by PC for more precise detection, can enhance monitoring effectiveness. This integrated strategy, along with the careful selection of RS data sources and analytical models, is crucial for improving the accuracy and reliability of cyanobacterial bloom monitoring, ultimately contributing to better water management and public health protection. Full article

Detecting targets in remote sensing imagery, particularly when identifying sparsely distributed materials, is crucial for applications such as defense, mineral exploration, agriculture, and environmental monitoring. The effectiveness of detection and the precision of the results are influenced by several factors, including sensor configurations, platform properties, interactions between targets and their background, and the spectral contrast of the targets. Environmental factors, such as atmospheric conditions, also play a significant role. Conventionally, target detection in remote sensing has relied on statistical methods that typically assume a linear process for image formation. However, to enhance detection performance, it is critical to account for the geometric and spectral variabilities across multiple imaging platforms. In this research, we conducted a comprehensive target detection experiment using a unique benchmark multi-platform hyperspectral dataset, where man-made targets were deployed on various surface backgrounds. Data were collected using a hand-held spectroradiometer, UAV-mounted hyperspectral sensors, and airborne platforms, all within a half-hour time window. Multi-spectral space-based sensors (i.e., Worldview and Landsat) also flew over the scene and collected data. The experiment took place on 23 July 2021, at the Rochester Institute of Technology’s Tait Preserve in Penfield, NY, USA. We validated the detection outcomes through receiver operating characteristic (ROC) curves and spectral similarity metrics across various detection algorithms and imaging platforms. This multi-platform analysis provides critical insights into the challenges of hyperspectral target detection in complex, real-world landscapes, demonstrating the influence of platform variability on detection performance and the necessity for robust algorithmic approaches in multi-source data integration. Full article

Soybean seed composition, particularly protein and oil content, plays a critical role in agricultural practices, influencing crop value, nutritional quality, and marketability. Accurate and efficient methods for predicting seed composition are essential for optimizing crop management and breeding strategies. This study assesses the effectiveness of combining handheld spectroradiometers with the Mexican Hat wavelet transformation to predict soybean seed composition at both seed and canopy levels. Initial analyses using raw spectral data from these devices showed limited predictive accuracy. However, by using the Mexican Hat wavelet transformation, meaningful features were extracted from the spectral data, significantly enhancing prediction performance. Results showed improvements: for seed-level data, Partial Least Squares Regression (PLSR), a method used to reduce spectral data complexity while retaining critical information, showed R² values increasing from 0.57 to 0.61 for protein content and from 0.58 to 0.74 for oil content post-transformation. Canopy-level data analyzed with Random Forest Regression (RFR), an ensemble method designed to capture non-linear relationships, also demonstrated substantial improvements, with R² increasing from 0.07 to 0.44 for protein and from 0.02 to 0.39 for oil content post-transformation. These findings demonstrate that integrating handheld spectroradiometer data with wavelet transformation bridges the gap between high-end spectral imaging and practical, accessible solutions for field applications. This approach not only improves the accuracy of seed composition prediction at both seed and canopy levels but also supports more informed decision-making in crop management. This work represents a significant step towards making advanced crop assessment tools more accessible, potentially improving crop management strategies and yield optimization across various farming scales. Full article

Proximal sensing has become increasingly popular due to developments in soil observation technologies and the demands of timely information gathering through contemporary methods. By utilizing the morphological, physical, and chemical characteristics of representative pedogenetic profiles established in various soils of the Sohag governorate, Egypt, the current research addresses the characterization of surface reflectance spectra and links them with the corresponding soil classification. Three primary areas were identified: recently cultivated, old cultivated, and bare soils. For morphological analysis, a total of 25 soil profiles were chosen and made visible. In the dark room, an ASD Fieldspec portable spectroradiometer (350–2500 nm) was used to measure the spectrum. Based on how similar their surface spectra were, related soils were categorized. Ward’s method served as the basis for the grouping. Despite the fact that the VIS–NIR spectra of the surface soils from various land uses have a similar reflectance shape, it is still possible to compare the soil reflectance curves and the effects of the surface soils. As a result, three groups of soil curves representing various land uses were observed. Cluster analysis was performed on the reflectance data in four ranges (350–750, 751–1150, 1151–1850, and 1851–2500 nm). The groups derived from the soil surface ranges of 350–750 nm and 751–1150 nm were not the same as those derived from the ranges of 1151–1850 nm and 1851–2500 nm. The last two categories are strikingly comparable to various land uses with marginally similar features. Based on the ranges of 1151–1850 nm and 1851–2500 nm in surface spectral data, the dendrogram effectively separated and combined the profiles into two separate clusters. These clusters matched different land uses exactly. The results can be used to promote the widespread usage of in situ hyperspectral data sets for the investigation of various soil characteristics. Full article

The application of hyperspectral data in machine learning models can contribute to the rapid and accurate determination of caffeine content in coffee beans. This study aimed to identify the machine learning algorithm with the best performance for predicting caffeine content and to find input data for these models that can improve the accuracy of these algorithms. The coffee beans were harvested one year after the seedlings were planted. The fresh beans were taken to the spectroscopy laboratory (Laspec) at the Federal University of Mato Grosso do Sul, Chapadão do Sul campus, for spectral evaluation using a spectroradiometer. For the analysis, the dried coffee beans were ground and sieved for the quantification of caffeine, which was carried out using a liquid chromatograph on the Waters Acquity 1100 series UPLC system, with an automatic sample injector. The spectral data of the beans, as well as the spectral data of the roasted and ground coffee, were analyzed using machine learning (ML) algorithms to predict caffeine content. Four databases were used as input: the spectral information of the bean (CG), the spectral information of the bean with additional clone information (CG+C), the spectral information of the bean after roasting and grinding (CGRG) and the spectral information of the bean after roasting and grinding with additional clone information (CGRG+C). The caffeine content was used as an output to be predicted. Each database was subjected to different machine learning models: artificial neural networks (ANNs), decision tree (DT), linear regression (LR), M5P, and random forest (RF) algorithms. Pearson’s correlation coefficient, mean absolute error, and root mean square error were tested as model accuracy metrics. The support vector machine algorithm showed the best accuracy in predicting caffeine content when using hyperspectral data from roasted and ground coffee beans. This performance was significantly improved when clone information was included, allowing for an even more accurate analysis. Full article