Search Results (253)

Antioxidants are critical components of the body’s defense system, providing protection against cell-damaging free radicals responsible for oxidative damage of biomolecules. Humans benefit from the consumption of plants with high antioxidant content, which have been shown to positively impact health. In plant physiology, antioxidants provide protection from biotic and abiotic stress, particularly during the development of seeds and germination. Peanut seeds and seed coats have been shown to contain several beneficial antioxidants and are a good source of phytonutrients. Seed coat color can vary greatly and impact the antioxidant capacity of the edible portion of the peanut. Additionally, the seed coat can provide bitter notes in products, affecting their palatability and potentially negating the beneficial properties of the antioxidants present. A total of 42 accessions from the Germplasm Resource Information Network (GRIN) with a variety of seed coat colors were obtained and analyzed for total antioxidant capacity to provide a baseline assessment of the distribution of antioxidants in kernel versus seed coats. The results demonstrated that seed coat color somewhat impacts antioxidant capacity, and 56–88% of the total antioxidant capacity resides in the seed kernel. Three control samples, not part of the germplasm collection, were roasted and prepared for analysis by the descriptive sensory panel. Seed coats were added back to the roasted paste in increasing proportion for analysis by the panel, and perceptions regarding bitterness and overall organoleptic properties were noted. Based on the results of this study, several accessions were selected and then planted for increase and potential crossbreeding with appropriate commercial cultivars. This information could be used to selectively add antioxidant capacity to peanut breeding programs to provide additional health benefits to consumers without compromising the sensory perception and desirability and peanut products in nutrition. Full article

Ecosystem services (ESs) are a life-support system for human development that are also a strategic root for realizing global ecological security and sustainable development. In this study, the spatial distribution pattern of land-use and ESs under three scenarios (an ecological protection scenario (EPS), a natural development scenario (NDS), and a cropland protection scenario (CPS)) in the Tarim River Basin (TRB), Northwest China, is predicted for 2035 using the Future Land-Use Simulation (FLUS)–Integrated Valuation of ESs and Trade-Offs (InVEST) model. Land-use data from 2000 to 2023 are utilized as the basic data, and the spatial and temporal characteristics of land-use and multiple ESs under different scenarios are explored. The results show that (1) the land-use structure of the TRB is dominated by barren land (55.12%) and grassland (30.28%), and the dynamic evolution of the land-use pattern from 2000 to 2023 is characterized by the continuous shrinkage of the area of barren land and the expansion of impervious surfaces, cropland, water bodies, and other productive and living land and water. (2) According to the prediction results of the FLUS model, the different scenarios of land-use for 2020–2035 show various change trends. In the EPS, the proportion of ecological land jumps to 35.23%, while production land and living land show a systematic contraction. Under the NDS, water bodies, grassland, and impervious surfaces experience a decreasing trend, whereas cropland, forest land, and barren land increase in area. Under the CPS, the trend of shrinkage for ecological land accelerates, especially the fragmentation of forest patches (shrinking by 24 km²) and the expansion of cropland and barren land. (3) A comparison and an analysis of the ESs in several scenarios for 2035 show an increase in ESs under the EPS compared with those in 2020, along with a marked improvement in the TRB’s future ecological environment under this scenario. By adhering to the guidance of ecological priority through optimization of the national spatial pattern and the integration of ecological elements, the dynamic balance between ecological protection and economic development can be effectively coordinated, providing core support for the sustainable development of the region. (4) Ecosystem services are significantly impacted by changes in grassland in a variety of settings, particularly in the NDS. Contradictory trade-offs between ecological functions are revealed in the CPS, where cropland expansion promotes soil conservation but worsens the degradation of grassland. In the EPS, the synergistic expansion of grassland and water favorably regulates ecosystem services. A major way to increase the capacity of regional ecosystem services and accomplish sustainable development is to optimize the land-use for ecological preservation, with an emphasis on increasing the acreage of grassland, forest, and water while decreasing the area of cropland and barren. Full article

Background/Objectives: Antimicrobial resistance (AMR) poses a critical global health threat, rendering common bacterial infections increasingly difficult to treat and placing considerable strain on healthcare systems. This study assesses public awareness, perceptions, and behaviors related to antibiotic use and AMR in Kosovo, a country with limited existing data on the topic. Methods: Using a cross-sectional survey design, 568 participants from diverse demographic backgrounds provided insights into their knowledge and practices concerning antibiotic use and antibiotic resistance. Results: The results revealed that although 75% of participants had heard of antibiotic resistance, only a limited proportion understood key terms. Knowledge of appropriate antibiotic use varied, with 67% of respondents correctly recognizing the need to complete a prescribed antibiotic course, while 29% believed it was acceptable to stop treatment once they felt better. Gender and educational level emerged as significant factors, with women and more educated individuals demonstrating greater awareness of proper antibiotic use and the risks of misuse. While 71% of respondents considered it unacceptable to use antibiotics prescribed to others, 41% believed it was acceptable to reuse previously effective antibiotics. Most participants (96%) reported obtaining antibiotics through prescriptions. Public awareness of AMR was generally high, but conceptual understanding remained limited, with misconceptions about the origins of resistance, incorrectly attributing it to the human body rather than bacteria. Conclusions: Targeted public health campaigns, guided by the One Health approach, integrating human, animal, and environmental health, are needed. A multifaceted strategy, including education, policy reforms, and international collaboration, is essential to mitigate AMR and preserve the efficacy of antibiotics for future generations. Full article

Background: The identification of body fluids collected from crime scenes is crucial for determining the type and nature of assaults and for advancing the resolution of crimes. Objectives: The primary aim of this study was to investigate tissue-specific DNA methylation markers that can effectively distinguish buccal samples from blood, semen, and vaginal epithelial tissue. Methods: We screened various markers and selected four genomic locations for further analysis. Genomic DNA was extracted from tissue samples, followed by bisulfite conversion, locus-specific polymerase chain reaction (PCR) amplification, and pyrosequencing. Results: Four loci—cg-9652652, cg-11536474, cg-3867465, and cg-10122865—along with several adjacent CpG sites, were found to be hypermethylated in buccal samples compared to other tissue types. The difference in DNA methylation of buccal samples was statistically significant (p < 0.0001) compared to other tissues, indicating the potential usefulness of these loci for forensic tissue identification. Two additional studies were conducted: (a) a species specificity study and (b) a mixture study involving two different tissue types. The species specificity study showed that the primers used in the assay were specific to primates and humans. They did not amplify five non-primate samples, while the two primate samples—chimpanzee and rhesus—provided usable methylation data. The mixture study involved DNA from two different tissues—buccal samples and semen—combined in varying proportions. The results showed a decrease in the overall percentage of DNA methylation at the locus cg-9652652 as well as five adjacent CpG sites when the amount of buccal cell DNA in the mixture was reduced. Conclusion: The specificity of the primers and the significant differences in percent DNA methylation between buccal cells and other tissues make these markers excellent candidates for forensic tissue identification. Full article

As the proportion of the elderly population increases, there is an urgent need for anti-aging technologies. Since the skin is the most visibly aging organ in the human body, it is crucial to develop active ingredients to slow down skin aging. Currently, identified anti-aging active substances include antioxidants, retinoids, peptides, growth factors, and compounds derived from biofermentation. However, they have limitations such as poor stability, low transdermal permeability, skin irritation, high effective concentrations, slow onset of efficacy, single-action mechanisms, and high production costs. These limitations highlight the necessity of developing new anti-aging technologies that are multifunctional and cause low irritation. This study aimed to investigate the anti-aging effects and mechanisms of fructosazine (FZ) and deoxyfructosazine (DOF) on the skin as well as their potential applications in skincare. The methods included ELISA tests to assess the viability of human dermal fibroblast (NHDF) cells and related factors, and monitoring in Sprague-Dawley (SD) rats. The results showed that FZ promoted cell viability. Both FZ and DOF enhanced the secretion of type I collagen (Col I) and hyaluronic acid (HA), inhibited matrix metalloproteinase-1 (MMP-1), boosted catalase (CAT), and reduced malondialdehyde (MDA), reactive oxygen species (ROS), and β-galactosidase. They also nourished the epidermis and increased fiber content. In conclusion, FZ and DOF can stimulate the production of anti-aging substances, exhibit antioxidant activity, and have potential in skincare. Full article

The pathogenesis of obesity is complex and incompletely understood, with an underlying interplay between our genetic architecture and obesogenic environment. The public understanding of the development of obesity is shrouded in myths with widespread societal misconceptions. Body Mass Index (BMI) is a highly heritable trait. However, despite reports from recent genome-wide association studies, only a small proportion of the overall heritability of BMI is known to be lurking within the human genome. Other non-genetic heritable traits may contribute to BMI. The gut microbiome is an excellent candidate, implicating complex interlinks with hypothalamic control of appetite and metabolism via entero-endocrine, autonomic, and neuro-humeral pathways. The neonatal gut microbiome derived from the mother via transgenerational transmission (vaginal delivery and breastfeeding) tends to have a permanence within the gut. Conversely, non-maternally derived gut microbiota manifest mutability that responds to changes in lifestyle and diet. We should all strive to optimize our lifestyles and ensure a diet that is replete with varied and unprocessed plant-based foods to establish and nurture a healthy gut microbiome. Women of reproductive age should optimize their gut microbiome, particularly pre-conception, ante- and postnatally to enable the establishment of a healthy neonatal gut microbiome in their offspring. Finally, we should redouble our efforts to educate the populace on the pathogenesis of obesity, and the role of heritable (but modifiable) factors such as the gut microbiome. Such renewed understanding and insights would help to promote the widespread adoption of healthy lifestyles and diets, and facilitate a transition from our current dispassionate and stigmatized societal approach towards people living with obesity towards one that is epitomized by understanding, support, and compassion. Full article

We analyse data from a rescue database collected at the Innsbruck Alpenzoo (Tyrol, Austria). The sample covers 33 years (1988–2020), and more than 5250 wild birds from 145 species originating from Innsbruck and the surrounding Inn Valley, one of the most densely populated areas in Europe. Both, the total number of birds as well as the number of bird species yearly admitted have increased since 1988. Orphaned nestlings and victims of glass collisions were the most common reasons for admission and responsible for the increase. Species’ susceptibility to accidental causes increased with regional abundance and degree of urbanisation. More urbanised species are characterised by a high proportion of nestlings and juveniles in the sample. The seasonal patterns of deliveries in these species show a peak in the late breeding season, and young birds are particularly susceptible to glass collisions and cat attacks. The species list also includes regionally rare wetland, upland and forest breeders and foreign migrants. Such species show a high proportion of admissions in autumn and collisions with windows play a greater role for short-distance migrants. Our data also suggest that small birds (<15 g body mass) are more likely to collide with glass panes than larger species. In conclusion, our data suggest that basically all bird groups and species are at least occasionally affected by human structures and activities in urbanised landscapes but support the notion that juveniles and migrants are more prone for accidents due to the lack of experience with anthropogenic structures in new areas. Full article

The objective of this study was to compare the effects of applying a 50% wheat grain diet with those of a diet with 3% additional dietary fibre from various sources on the development of broiler chickens’ gastrointestinal tract and its related organs and to model this phenomenon based on data obtained from 35-day-old chickens using multiple regression equations. The use of various structural components, including oat hull (OH), sunflower hull (SH), sugar beet pulp (SBP), and wheat bran (WB), in proportions of 3% of the diet not only affects digestive processes in broiler chickens’ gastrointestinal tract but also causes a change in the length of their intestinal sections or the weight of related organs. These effects can be taken into account when creating an experimental model, the results of which can at least be partially applicable to human studies. The use of OH and SH (3%) in the birds’ diets resulted in a significantly higher body weight (p < 0.05) compared with the use of SBP and WB. OH in the diet significantly increased (p < 0.01) the weight of the chicken’s gizzards compared with the other dietary fibre sources, apart from SH. On the other hand, the weight of the proventriculus in chickens fed the diet containing OH was significantly lower than that of the chickens fed the diet containing SBP (p < 0.05). The use of SH in the diet caused a significant decrease (p < 0.01) in the weight of the chickens’ heart. Compared with other additional sources of dietary fibre, OH in the diet also significantly increased (p < 0.05) the lengths of the small and large intestines, as well as the total length of the intestines. A correlation analysis showed a significant, average, positive relationship (p < 0.05) between the content of TDF in the diet and the weight of the gizzard and indicated a significant positive correlation between the lengths of the jejunum and the remaining sections of the intestines. Additionally, the regression equation models indicated a significant effect (p < 0.01) of all the independent variables on the jejunal, ileal, and caecal lengths and the liver weight. The application of the regression model confirmed significant changes in the small intestine and liver weight depending on the type of dietary fibre and other independent variables, which can also be taken into account when assessing diseases in people with thin intestines. However, further studies with separate models still need to be conducted using experiments including both soluble and insoluble fibre. Full article

Fluoride exposure has been shown to affect immune cell subsets and immune function, but its impact on macrophage polarization remains unclear. This study investigates the effects of low fluoride exposure on macrophage polarization and its underlying mechanisms through epidemiological surveys, animal experiments, and in vitro cell experiments. In the population-based epidemiological survey, we used mass cytometry to assess the impact of low fluoride exposure (0.570–2.027 mg/L) in the environment on human immune cell populations following the current water improvement and fluoride reduction measures. A rat fluorosis model was established by treating rats with sodium fluoride (NaF) in drinking water at concentrations of 0 mg/L, 5 mg/L, 10 mg/L, 25 mg/L, and 50 mg/L for 90 days., and morphological changes were assessed by hematoxylin–eosin (H&E) staining and transmission electron microscopy in the spleen of rats. Flow cytometry was used to analyze the proportion of macrophage subtypes in the spleen, while Western blot and immunofluorescence were performed to detect the expression of mitochondrial autophagy-related proteins. An M1 macrophage model was constructed in vitro by inducing THP-1 cells, and the effects of fluoride on macrophage-related cell markers and cytokines were assessed using flow cytometry and ELISA, respectively, following intervention with an autophagy inhibitor. Mitochondrial membrane potential and mitochondrial–lysosomal colocalization are analyzed through flow cytometry and confocal microscopy. The study aims to investigate the role of mitophagy in sodium fluoride-induced macrophage polarization. Epidemiological investigations revealed that low fluoride increases the proportion of blood monocytes, as well as the expression levels of CD68 (a macrophage surface marker), CD86 (an M1 macrophage marker), and the inflammatory cytokine IFN-γ in peripheral blood mononuclear cells (PBMCs). In the rats of NaF-treated groups, splenic tissues exhibited inflammatory infiltration, mitochondrial swelling, and increased autophagosome formation. Moreover, low fluoride activated the PINK1/Parkin-mediated mitophagy pathway, promoting an increase in the M2/M1 macrophage ratio. In vitro experiments further confirmed that autophagy inhibitors reversed the NaF-induced increase in the M2/M1 macrophage ratio. This study demonstrates that low fluoride induces inflammatory responses in the body and drives M1 macrophage polarization toward M2 macrophages via mitophagy. These findings highlight the potential immunological risks associated with low fluoride and provide mechanistic insights into the interplay among fluoride, mitophagy, and macrophage polarization. Full article

Background/Objectives: Very preterm body composition at term shows potential as a biomarker of later health outcomes, but effects from in-hospital formula versus human milk (HM) (maternal milk (MM) and/or pasteurised human donor milk (DM) supplement) intake studies are confounded by the effect from the fortifier. We investigated the impact of in-hospital unfortified HM (UHM), fortified HM (FHM), and preterm formula (PTF) intake on very preterm body composition at term. Methods: Preplanned analysis of the PREterM FOrmula or Donor milk (PREMFOOD) trial: Infants born at <32 weeks were randomised to either (i) UHM, (ii) FHM, or (iii) MM and/or PTF supplement. Main outcomes were assessed by anthropometry and magnetic resonance imaging of body composition at term. Secondary comparison between groups defined by proportion of milk intake from birth to 35 weeks postmenstrual age: The groups comprised exclusive UHM (ExUHM, proportion of UHM 99–100%, n = 23), predominantly UHM (PrUHM, UHM 50–98.9%, n = 15), predominantly FHM (PrFHM, FHM > 50%, n = 17), and predominantly PTF (PrPTF, PTF > 50%, n = 7). Results: At term, compared to the ExUHM group, the PrPTF group had 274.3 g (95% CI: 30.1 to 518.5) more Non-Adipose Tissue Mass (NATM) and a 1.11 times (95% CI: 0.38 to 1.84) greater increase in weight z score from birth, while both PrPTF and PrFHM had greater increases in length z scores from birth. Conclusions: High formula intake was associated with maximal gains in NATM at term, and these gains were not matched by the early fortification of HM. The alteration of body composition at term with prolonged or delayed HM fortification and its relation to later health outcomes are important research questions. Full article

The interaction of estrogen receptor alpha (ERα) with various metabolites—both endogenous and exogenous, such as those present in food products, as well as gut microbiota-derived metabolites—plays a critical role in modulating the hormonal balance in the human body. In this study, we evaluated a suite of 27 machine learning models and, following systematic optimization and rigorous performance comparison, identified linear discriminant analysis (LDA) as the most effective predictive approach. A meticulously curated dataset comprising 75 molecular descriptors derived from compounds with known ERα activity was assembled, enabling the model to achieve an accuracy of 89.4% and an F1 score of 0.93, thereby demonstrating high predictive efficacy. Feature importance analysis revealed that both topological and physicochemical descriptors—most notably FractionCSP3 and AromaticProportion—play pivotal roles in the potential binding to ERα. Subsequently, the model was applied to chemicals commonly encountered in food products, such as indole and various phenolic compounds, indicating that approximately 70% of these substances exhibit activity toward ERα. Moreover, our findings suggest that food processing conditions, including fermentation, thermal treatment, and storage parameters, can significantly influence the formation of these active metabolites. These results underscore the promising potential of integrating predictive modeling into food technology and highlight the need for further experimental validation and model refinement to support innovative strategies for developing healthier and more sustainable food products. Full article

In addition to various physiological parameters that affect athletes’ performance and outcomes, anthropometric variables are also related to athletic performance. In particular, the length of the lower limbs is closely associated with human mobility and stride length, making it a crucial factor in various movement-based sports. Furthermore, favorable body proportions may vary depending on the sport, and a better understanding of how body proportions affect physical performance across different levels of athletes is needed. Therefore, the purpose of this study is to investigate the relationship between leg length and physical performance by measuring body dimensions (tibia and femur length) for athletes categorized by sports characteristics and performance levels. The study involved 312 athletes from 23 sports, divided into three activity levels. Anthropometric measurements of tibia and femur length were taken, and physical performance tests, including strength, muscular endurance, cardiovascular endurance, agility, explosiveness, flexibility, and anaerobic power, were conducted in the laboratory. The relationships between variables (leg length × physical performance) were analyzed using Pearson’s correlation coefficients. Leg length via activity levels was verified through one-way analysis of variance (ANOVA) testing, including normality and homoscedasticity. Post hoc analysis (Tukey’s HSD test) was used to compare specific differences when significance was found. Statistical significance was accepted at the 0.05 level. As a result, an increase in lower limb length was found to have a relationship with physical performance components, including power (r = 0.302, p = 0.001), agility (r = −0.289, p = 0.001), endurance capacity (r = 0.168, p = 0.005), and anaerobic peak power (r = 0.265, p = 0.001). However, in the LD group, which consisted of athletes in static sports, no significant relationship was observed between lower limb length and physical performance components. However, in the LD group, which included static sports, no significant relationship was found between lower limb length and physical performance components. These findings may serve as foundational data for athlete talent identification and performance prediction. Full article

While most Christians might imagine the resurrection of Jesus Christ from the dead as a single event, for the author of Ephesians, the resurrection is a continuing event of cosmic proportions. In a very real way, the Epistle to the Ephesians is an extended reflection on the ongoing multidimensional cosmic consequences and transformations that result from the death of Jesus and his resurrection, whose impact not only affects the macrocosm in which Christ sits triumphantly at the right hand of God, “far above every principality, authority, power, and dominion” (Eph 1: 20–22), but also the microcosm of the Church, “his body, the fullness of the one who fills the universe in every way” (1: 23), transforming those who compose the smallest microcosm, the baptized who form a Christian household and who, gathered at table to share Eucharist (5: 17–6: 9), are “seated with Christ in the heavenly places” (2: 6), already participating in the eternal Messianic banquet. This is to say that, for this author, the resurrection of Jesus of Nazareth is the catalyst for an ongoing and ever more evolving “new creation” of humanity and, indeed, the entire cosmos, with “Christification”—the full maturation into the divine “Christ nature” (Eph 4: 13, 15–16) as the telos or goal for the whole universe (Eph 1: 10). Full article

$\beta$-hydroxy-$\beta$-methylbutyrate (HMB) has been shown to enhance muscle function and strength in older humans and rodents after periods of consumption extending for several weeks. We investigated the feasibility of utilizing Drosophila as a model organism to study the biological effects of HMB on aging muscle when consumed throughout adult life. Using flight ability as an index of flight muscle function, we found that HMB attenuates the age-dependent decline in flight ability. Male and female flies fed a diet supplemented with 10 mg/mL HMB had significantly higher flight scores from median age until the onset of flight senescence than control flies fed a standard diet. HMB supplementation also resulted in improved flight scores in males before median age and delayed the onset of flight senescence in females. Notably, the consumption of HMB throughout adult life increased the rate of survival and extended lifespan. The effect on lifespan did not result from changes in food consumption or body weight. Old flies on the HMB-supplemented diet retained a higher proportion of flight muscle mitochondria whose morphology resembled that of young flies than the control diet group. Together, these results suggest that HMB attenuates the age-dependent decline in flight ability and prolongs lifespan by enhancing muscle health. Full article

In complex electromagnetic environments, cardiac pacemakers may be interfered with easily. Microwave ovens, as common household appliances, may display electromagnetic leakage, which may pose risks to pacemaker wearers. This work evaluates the electromagnetic exposure of pacemaker wearers under various conditions. One involves different distances from the microwave oven to the human body, and the other involves a distinct oven door gap. This work uses COMSOL Multiphysics to establish a human thoracic cavity model with a heart and unipolar pacemaker, as well as a model of a microwave oven with contact-type doors. The results show that the specific absorption rate (SAR_10g) and temperature increase in the thoracic cavity and heart tissue are inversely proportional to the distance from the microwave source. They are directly proportional to the oven door gap size. The induced electric field intensity, the temperature increase, and the induced voltage in the pacemaker show the same trend. When the human body is closest to the microwave oven with the largest door gap (D = 100 mm, d = 0.3 mm), the SAR_10g and temperature increase of the thoracic cavity and heart tissue reach their maximum values, which are significantly below the safety standards recommended by ICNIRP. Similarly, the maximum value of the temperature increase and the induced electric field intensity in the pacemaker are below the safety standard recommended by ISO 14708-3 (+2 °C) and IEC 60601-1-2 (28 V/m). The maximum induced voltage at the pacemaker electrode is 5.322 mV, which exceeds the sensing sensitivity setting recommended by ISO 14117 (2 mV) for unipolar pacemakers. These findings demonstrate that microwave ovens with contact-type doors electromagnetic radiation do not threaten human health under normal usage conditions. However, the maximum value of the induced voltage exceeds the sensing sensitivity of some unipolar pacemakers, which may affect the operation of the unipolar pacemaker. This phenomenon requires attention from clinicians and patients. We still recommend that pacemaker wearers keep a distance from microwave ovens when using them. Full article