Search Results (506)

(1) Background: The goal of the present study was to evaluate whether the supplementation with a multi-species probiotic in the diet of laying hens can change the microbiota and health status of the oviduct. (2) Methods: A total of 60 cages housing lightweight laying hens (36 weeks old) were randomly assigned to the following two different treatments: a control group fed a diet without probiotic, and a treatment group receiving diets supplemented with 50 g/ton of probiotics. The trial lasted for 26 weeks, after which five layers were slaughtered per treatment for oviduct (magnum) assessment, focusing on microbiome composition, oxidant and antioxidant status, and morphological analyses. Additionally, intestinal (jejunum) samples were collected to determine oxidant and antioxidant status. (3) Results: Probiotic supplementation resulted in lower counts of organisms from the RB41 order (p = 0.039) and Burkholderia genus (p = 0.017), and a total reduction in Bacillus and Corynebacterium (p = 0.050) compared to the control treatment. Genera Burkholderia (p = 0.017), Corynebacterium (p = 0.050), and Bacillus (p = 0.050) were also lower with the probiotic supplementation in relation to the control. Genera Epulopiscium (p = 0.089), Flavobacterium (p = 0.100), Ruminococcus (p = 0.089), and Staphylococcus (p = 0.100) tended to be lower in the probiotic group compared to the control. No significant differences were found between treatments for oviduct lesions. Probiotic treatment resulted in a higher protein thiol level in the intestine compared to the control (p < 0.001). However, the use of probiotics tended to reduce glutathione S-transferase levels in the oviduct compared to the control (p = 0.068). (4) Conclusions: These results suggest that dietary supplementation with probiotics can modulate the oviduct microbiota and improve the antioxidant status of laying hens, without causing tissue damage. Further research is warranted to explore the long-term implications of these changes on reproductive performance and egg quality. Full article

The 2020 southern Puerto Rico earthquake sequence highlighted the severe seismic vulnerability of informally constructed two-story reinforced concrete (RC) houses. This study examines the failure mechanisms of these structures and assesses the effectiveness of first-floor RC shear-wall retrofitting. Nonlinear pushover and dynamic time–history analyses were performed using fiber-based distributed plasticity models for RC frames and nonlinear macro-elements for second-floor masonry infills, which introduced a significant inter-story stiffness imbalance. A bi-directional seismic input was applied using spectrally matched, near-fault pulse-like ground motions. The findings for the as-built structures showed that stiffness mismatches between stories, along with substantial strength and stiffness differences between orthogonal axes, resulted in concentrated plastic deformations and displacement-driven failures in the first story—consistent with damage observed during the 2020 earthquakes. Retrofitting the first floor with RC shear walls notably improved the performance, doubling the lateral load capacity and enhancing the overall stiffness. However, the retrofitted structures still exhibited a concentration of inelastic action—albeit with lower demands—shifted to the second floor, indicating potential for further optimization. Full article

Housing sustainability is a cornerstone element of sustainable economic and social development. This is particularly true for China, where high-rise residential buildings are the primary form of housing. In recent years, China has experienced frequent housing-related accidents, resulting in a significant loss of life and property damage. This study aims to identify the key factors influencing housing sustainability and provide a basis for the prevention and control of housing-related safety risks. This study has developed a housing sustainability evaluation indicator system comprising three primary indicators and 16 secondary indicators. This system is based on an analysis of the causes of over 500 typical housing accidents that occurred in China over the past 10 years, employing research methods such as literature reviews and expert consultations, and drawing on the analytical frameworks of risk management theory and system safety theory. Subsequently, the NK-SNA model, which significantly outperforms traditional models in terms of adaptive learning and optimization, as well as the explicit modeling of complex nonlinear relationships, was used to identify the key risk factors affecting housing sustainability. The empirical results indicate that the risk coupling value is correlated with the number of risk coupling factors; the greater the number of risk coupling factors, the larger the coupling value. Human misconduct is prone to forming two-factor risk coupling with housing, and the physical risk factors are prone to coupling with other factors. The environmental factors easily trigger ‘physical–environmental’ two-factor risk coupling. The key factors influencing housing sustainability are poor supervision, building facilities, the main structure, the housing height, foundation settlement, and natural disasters. On this basis, recommendations are made to make full use of modern information technologies such as the Internet of Things, big data, and artificial intelligence to strengthen the supervision of housing safety and avoid multi-factor coupling, and to improve upon early warnings of natural disasters and the design of emergency response programs to control the coupling between physical and environmental factors. Full article

The modern Chinese architectural heritage combines sturdy Western materials with delicate Chinese styling, mainly adopting brick-timber structural systems that are highly vulnerable to fire damage. The study assesses the fire spread characteristics of the First Faculty House, a 20th-century architectural heritage located at a university in China. The assessment is carried out by analyzing building materials, structural configuration, and fire load. By using FDS (Fire Dynamics Simulator (PyroSim version 2022)) and SketchUp software (version 2023) for architectural reconstruction and fire spread simulation, explores preventive measures to reduce fire risks. The result show that the total fire load of the building amounts to 1,976,246 MJ. After ignition, flashover occurs at 700 s, accompanied by a sharp increase in the heat release rate (HRR). The peak ceiling temperature reaches 750 °C. The roof trusses have critical structural weaknesses when approaching flashover conditions, indicating a high potential for collapse. Three targeted fire protection strategies are proposed in line with the heritage conservation principle of minimal visual and functional intervention: fire sprinkler systems, fire retardant coating, and fire barrier. Simulations of different strategies demonstrate their effectiveness in mitigating fire spread in elongated architectural heritages with enclosed ceiling-level ignition points. The efficacy hierarchy follows: fire sprinkler system > fire retardant coating > fire barrier. Additionally, because of chimney effect, for fire sources located above the ceiling and other hidden locations need to be warned in a timely manner to prevent the thermal plume from invading other sides of the ceiling through the access hole. This research can serve as a reference framework for other Modern Chinese Architectural Heritage to develop appropriate fire mitigation strategies and to provide a methodology for sustainable development of the Chinese architectural heritage. Full article

As high-speed electric multiple units (EMUs) advance in speed and complexity, quasi-static design methods may underestimate the fatigue risks associated with high-frequency dynamic excitations. This study quantifies the contribution of gear meshing-induced vibrations (2512 Hz) to fatigue damage in EMU gearbox housings, revealing resonance amplification of local stresses up to 1.8 MPa at 300 km/h operation. Through integrated field monitoring and bench testing, we demonstrated that gear meshing excites structural modes, generating sustained, very high-cycle stresses (>10⁸ cycles). Crucially, fatigue specimens were directly extracted from in-service gearbox housings—overcoming the limitations of standardized coupons—passing the very high-cycle fatigue (VHCF) test to derive S-N characteristics beyond 10⁸ cycles. Results show a continuous decline in fatigue strength (with no traditional fatigue limit) from 10⁸ to 10⁹ cycles. This work bridges the gap between static design standards (e.g., FKM) and actual dynamic environments, proving that accumulated damage from low-amplitude gear-meshing stresses (3.62 × 10¹¹ cycles over a 12 million km lifespan) contributes to a 16% material utilization ratio. The findings emphasize that even low-magnitude gear-meshing stresses can significantly influence gearbox fatigue life due to their ultra-high frequency, warranting design consideration beyond current standards. Full article

We present a miniaturized, inexpensive, and user-friendly microfluidic platform to support biological applications. The system integrates a mini-incubator providing controlled environmental conditions and housing a microfluidic device for long-term cell culture experiments. The incubator is designed to be compatible with standard inverted optical microscopes and Raman spectrometers, allowing for the non-invasive imaging and spectroscopic analysis of cell cultures in vitro. The microfluidic device, which reproduces a dynamic environment, was optimized to sustain a passive, gravity-driven flow of medium, eliminating the need for an external pumping system and reducing mechanical stress on the cells. The platform was tested using Raman analysis and adherent tumoral cells to assess proliferation prior and subsequent to hydrogen peroxide treatment for oxidative stress induction. The results demonstrated a successful adhesion of cells onto the substrate and their proliferation. Furthermore, the platform is suitable for carrying out optical monitoring of cultures and Raman analysis. In fact, it was possible to discriminate spectra deriving from control and hydrogen peroxide-treated cells in terms of DNA backbone and cellular membrane modification effects provoked by reactive oxygen species (ROS) activity. The 800–1100 cm⁻¹ band highlights the destructive effects of ROS on the DNA backbone’s structure, as its rupture modifies its vibration; moreover, unpaired nucleotides are increased in treated sample, as shown in the 1154–1185 cm⁻¹ band. Protein synthesis deterioration, led by DNA structure damage, is highlighted in the 1257–1341 cm⁻¹, 1440–1450 cm⁻¹, and 1640–1670 cm⁻¹ bands. Furthermore, membrane damage is emphasized in changes in the 1270, 1301, and 1738 cm⁻¹ frequencies, as phospholipid synthesis is accelerated in an attempt to compensate for the membrane damage brought about by the ROS attack. This study highlights the potential use of this platform as an alternative to conventional culturing and analysis procedures, considering that cell culturing, optical imaging, and Raman spectroscopy can be performed simultaneously on living cells with minimal cellular stress and without the need for labeling or fixation. Full article

Urban pluvial flooding (UPF) is an increasingly critical issue due to rapid urbanization and intensified precipitation driven by climate change that yet remains understudied in the Caribbean. This study analyzes the effects of UPF resulting from the transformation of a natural karstic landscape into an urbanized area considering a sub-watershed in Chetumal, Southern Mexican Caribbean, as a case study. Hydrographic numerical modeling was conducted using the IBER 2.5.1 software and the SCS-CN method to estimate surface runoff for a critical UPF event across three stages: (i) 1928—natural condition; (ii) 1998—semi-urbanized (78% coverage); and (iii) 2015—urbanized (88% coverage). Urbanization led to the orthogonalization of the drainage network, an increase in the sub-watershed area (20%) and mainstream length (33%), flow velocities rising 10–100 times, a 52% reduction in surface roughness, and a 32% decrease in the potential maximum soil retention before runoff occurs. In urbanized scenarios, 53.5% of flooded areas exceeded 0.5 m in depth, compared to 16.8% in non-urbanized conditions. Community-based knowledge supported flood extent estimates with 44.5% of respondents reporting floodwater levels exceeding 0.50 m, primarily in streets. Only 43.1% recalled past flood levels, indicating a loss of societal memory, although risk perception remained high among directly affected residents. The reported UPF effects perceived in the area mainly related to housing damage (30.2%), mobility disruption (25.5%), or health issues (12.9%). Although UPF events are frequent, insufficient drainage infrastructure, altered runoff patterns, and limited access to public shelters and communication increased vulnerability. Full article

In the environment, plastic waste degrades into small particles known as microplastics and nanoplastics (MNPLs), depending on their size. Given the potential harmful effects associated with MNPL exposure, it is crucial to develop environmentally representative particles for hazard assessment. These so-called true-to-life MNPLs are generated through in-house degradation of real-world plastic products. In this study, we produced titanium-doped nanoplastics (NPLs) from opaque polyethylene terephthalate (PET) milk bottles, which contain titanium dioxide as a filler. The resulting PET(Ti)-NPLs were thoroughly characterized using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), mass spectrometry (MS), dynamic light scattering (DLS), ζ-potential measurements, transmission electron microscopy (TEM), and Fourier-transform infrared (FTIR) spectroscopy. Human-derived THP-1 monocytes were employed to investigate particle uptake kinetics, dosimetry, and genotoxicity. A combination of flow cytometry and inductively coupled plasma mass spectrometry (ICP-MS) enabled the quantification of internalized particles, while the comet assay assessed DNA damage. The results revealed dose- and time-dependent effects of PET(Ti)-NPLs on THP-1 cells, particularly in terms of internalization. Titanium doping facilitated detection and influenced genotoxic outcomes. This study demonstrates the relevance of using environmentally representative nanoplastic models for evaluating human health risks and underscores the importance of further mechanistic research. Full article

This paper conducts a full-scale shaking table test on a prototype prefabricated L-shaped column concrete house structure to experimentally verify its seismic performance in high-intensity seismic areas. We analyze the frequency of the structure, story drift angle, amplification factor of peak acceleration of ground motion, and damage distribution. The corresponding finite element model is established using ABAQUS to verify the experimental results and further study the damage forms and weak areas of the structure under a strong earthquake. The results show that the structure can maintain an elastic state under the rare intensity of a level 7 earthquake, but begins to bend and deform in the direction of the two main axes under the rare intensity of a level 8 earthquake. At a rare intensity of a level 9 earthquake, the structure is completely destroyed. This result shows that the unitary precast special-shaped column concrete structure meets the requirements of the current code. Full article

Electricity consumption is increasing as a result of increasing people’s needs, such as lighting, heating, and comfort. Different needs come into play day by day in the houses where people live and in places used as common areas, and this increases the need for electricity. Studies have observed that almost half of the world’s electricity consumption is made by buildings. Public buildings, shopping malls, hospitals, and hotels are typical examples of such structures. However, hospitals have an important place among all building types as they contain a wide range of devices and are of critical importance to many systems. Consumption in hospitals is a necessity rather than a desire for comfort in places such as hotels and shopping malls. Therefore, analysis of the energy consumed by hospitals is one of the important things to perform to reduce the damage caused by electricity consumption to the environment. In this study, the energy analysis of a green hospital with an installed area of 55,000 square meters in Istanbul was conducted, and machine learning techniques can be used in the analysis. Among many methods used for building energy analysis, long short-term memory (LSTM) has been chosen. The available data set was analyzed with the various LSTM methods and classification and prediction operations were carried out. Error rates for each method were compared. With the results obtained, it has been observed that the vanilla LSTM method provides acceptable results in building energy analysis. Full article

Fear is a critical welfare concern in laying hens. Fearful behaviors in domestic chickens are influenced by both genetic and environmental factors, contributing to individual differences in stress responses. Tonic immobility (TI) duration is widely recognized as a reliable indicator of fear levels. The hippocampus, a critical brain region for emotional states, plays a pivotal role in associating fearful experiences with specific stimuli, enabling adaptive behavioral responses. This study investigated hippocampal histological characteristics and transcriptomic profiles in laying hens with different fear responses categorized based on TI duration. A total of 80 native Lindian hens (75 weeks old) were individually housed in modified conventional cages. At 76 weeks of age, hens exhibiting the longest and shortest TI durations were classified into the high-fear (TH) and low-fear (TL) groups, respectively. Whole hemibrains were collected for histological and immunohistochemical analyses, while hippocampal tissues underwent transcriptome sequencing. The results showed a significant reduction in Nissl body counts in hippocampal neurons of high-fear hens (p < 0.05), suggesting potential neuronal damage or functional impairment. Transcriptomic analysis revealed 365 differentially expressed genes (DEGs) between two groups, with 277 upregulated and 88 downregulated genes in TH chickens. KEGG pathway enrichment analysis identified seven significantly associated pathways (p < 0.01), including retinol metabolism, vitamin B6 metabolism, and nicotinate and nicotinamide metabolism, all of which are crucial for neuronal function and immune regulation. In addition, a significant increase in DCX protein expression (p < 0.05) and a decrease in c-Fos protein expression (p < 0.05) was noted in in high-fear hens, whereas PCNA levels remained unchanged (p > 0.05) under immunohistochemical validation. The neuronal alterations observed in high fear individuals suggest neural damage, while transcriptomic variations point to potential disruptions in neurogenesis, synaptic signaling, and stress-related pathways. Collectively, these results provide novel insights into the neurobiological basis of fear regulation in laying hens and may have implications for poultry welfare and management strategies. Full article

Housing recovery represents a critical component of disaster recovery, and accurately forecasting household relocation decisions is essential for guiding effective post-disaster reconstruction policies. This study explores the use of machine learning algorithms to improve the prediction of household relocation in the aftermath of disasters. Leveraging data from 1304 completed interviews conducted as part of the Displaced New Orleans Residents Survey (DNORS) following Hurricane Katrina, we evaluate the performance of Logistic Regression (LR), Random Forest (RF), and Weighted Support Vector Machine (WSVM) models. Results indicate that WSVM significantly outperforms LR and RF, particularly in identifying the minority class of relocated households, achieving the highest F1 score. Key predictors of relocation include homeownership, extent of housing damage, and race. By integrating variable importance rankings and partial dependence plots, the study also enhances interpretability of machine learning outputs. These findings underscore the value of advanced predictive models in disaster recovery planning, particularly in geographically vulnerable regions like New Orleans where accurate relocation forecasting can guide more effective policy interventions. Full article

Controlling low ambient temperatures and ammonia levels is critical for effective environmental management in poultry houses during winter, as both represent persistent stressors affecting bird health and productivity. However, evidence regarding their combined long-term effects on the physiological responses of laying hens remains limited. In this study, 576 eighteen-week-old Hy-Line Brown hens were randomly assigned to six treatments (8 replicates with 12 birds per replicate each treatment) and housed in environmentally controlled chambers for 20 weeks: T1 (8 °C, ≤5 ppm ammonia), T2 (8 °C, 20 ppm ammonia), T3 (8 °C, 45 ppm ammonia), T4 (20 °C, ≤5 ppm ammonia; control), T5 (20 °C, 20 ppm ammonia), and T6 (20 °C, 45 ppm ammonia). Plasma samples were collected at 22, 26, 30, 34, and 38 weeks to evaluate physiological stress biomarkers (corticosterone, CORT; total antioxidant capacity, T-AOC), immunoglobulins (IgG, IgM, and IgA), and reproductive hormones (luteinizing hormone, LH; follicle-stimulating hormone, FSH; estradiol, E2). At 38 weeks, hypothalamus, pituitary, and spleen tissues were collected to assess the relative mRNA expression of gonadotropin-releasing hormone (GnRH), FSH, tumor necrosis factor-α (TNF-α), and interleukins (IL-1β, IL-6, and IL-10). Results showed that both cold and ammonia stress reduced antioxidant capacity, disrupted immune homeostasis, and altered reproductive hormone profiles. Cold exposure induced acute immunoendocrine alterations with partial physiological adaptation over time, whereas ammonia exerted progressive and cumulative damage, including elevated immunoglobulins (IgG and IgM) and downregulation of GnRH and FSH expression. Combined exposure significantly upregulated TNF-α and IL-1β expression, suggesting a synergistic inflammatory response. These results highlight complex, parameter-specific interactions between cold and ammonia stressors, emphasizing the need for targeted environmental strategies. Stage-specific interventions—thermal regulation in early laying and ammonia control in later phases—are recommended to safeguard hen health and optimize productivity under winter conditions. Full article

The effects of stocking density (SD) and feeder access (FA) on the performance and damaging behaviour of weaned piglets are not well understood. Three experiments (Ts) were conducted. T1 compared low SD (LSD) (0.55 m²/piglet) and moderate SD (MSD) (0.37 m²/piglet), T2 compared low FA (LFA1) (1.33 piglets/feeder; partially slatted floor) and high FA (HFA1) (0.66 piglets/feeder; fully slatted floor), and T3 compared LFA2 (2.33 piglets/feeder) and HFA2 (1.00 piglets/feeder). T1 included 24 replicates, while T2 and T3 each had 12 replicates. In total, 576 piglets were weighed at days (d) 0, 14, and 35 post-weaning (PW). Faecal consistency (FC) was recorded multiple times per week. Tail and ear injuries were scored at d35 PW. Piglets housed under LSD grew faster throughout the experiment (p < 0.05) and had a lower feed conversion ratio (FCR) between d0 and 14 PW (p = 0.02) than those in MSD. Tail injuries were not significantly affected (p = 0.14). HFA1 piglets had higher FC between d0 and 14 PW (p = 0.01) and overall (p = 0.02), but fewer intact tails at d35 PW (p = 0.01) compared with LFA1. In T3, LFA2 piglets had a lower average daily gain (p = 0.03), higher FCR between d14 and 35 PW (p = 0.04), and fewer intact tails (p < 0.01) than HFA2 piglets. These results suggest that both FA and SD can influence piglet performance, FC, and damaging behaviour. Full article

Infectious bursal disease (IBD) is a viral disease that most commonly affects young chickens and destroys lymphocytes, leading to immunosuppression. The field study aimed to investigate the effect of three different vaccines administered in ovo against IBD and spray against Newcastle disease (ND) on serological response tested for IBD and ND and histopathological analysis of the bursa of Fabricius (BF) and quantitative B lymphocytes in BF in broiler chickens. The study was conducted on a farm of four hen houses with 30,000 chicks in each building. Three different vaccination programs were used in the poultry hatchery, and one hen house IV was not vaccinated. All three groups were vaccinated at 18 days and 9 h in ovo during egg transfer against IBD at a dose of 0.05 mL/embryo, group I vector vaccine (strain vHVT013-69), group II immunocomplex vaccine (strain Winterfield 2512), group III immunocomplex vaccine (strain M.B, 0.05). Then, after hatching, the chicks were vaccinated in a spray (groups I, II, and III) against NDV (strain VG/GA, 20 mL/100 birds) and infectious bronchitis (IBV) in a spray (strain H-120, serotype Mass, and strain CR88121, serotype 793B) at a dose of 20 mL/100 chicks. On days 1, 21, 31, and 41, blood was collected for serological tests to determine the antibody titer against IBD, which was performed using two tests (IDEXX and ID-Vet) and against ND. During the necropsy of birds on days 21 and 31, the bursae of Fabricius were collected from five chickens for histopathological evaluation of BF and quantitative B lymphocyte counts; a total of 40 bursae were analyzed (10 per group). The vaccination program applied significantly (p < 0.05) affected the immune response expressed as a geometric mean titer (GMT) in the serum of the examined chickens against IBDV on days 21, 31, and 41. Differences were also demonstrated in the mass and level of BF damage and the number of B lymphocytes. No significant differences were demonstrated in the GMT in the serum of the examined chickens against NDV depending on the vaccination program applied. Full article