Search Results (56)

In this study, honey samples collected from the Varda Plateau (İkizdere Valley, Rize) and Yusufeli regions of northeastern Türkiye were comprehensively characterized in terms of their melissopalynological, physicochemical, elemental, and volatile properties. Ten honey samples collected from different apiaries were analyzed. Melissopalynological results identified two samples as monofloral Castanea sativa (chestnut) honeys and three samples as Myosotis-dominant honeys, while the remaining samples were classified as multifloral. The pollen spectra reflected the rich floristic diversity of the region, shaped by both forest vegetation and high-altitude herbaceous flora. Moisture and proline analyses indicated that the investigated samples were consistent with the characteristics of natural and well-matured honeys. Volatile compound analysis by HS-SPME/GC–MS revealed substantial qualitative and quantitative differences among samples, with chestnut and Myosotis honeys generally exhibiting more distinctive aroma profiles than multifloral honeys. Benzaldehyde, furfural, nonane, and linalool derivatives were among the most frequently detected volatile compounds, although their relative abundances varied considerably among samples. Elemental analysis identified potassium as the predominant mineral in all honey samples, while significant variability was observed for Fe, Zn, Mn, Ni, and Cu concentrations. Particularly, the Yusufeli samples were characterized by markedly higher Fe and Cu contents than the Varda samples, indicating a strong geographical influence on elemental composition. Multivariate analyses, including PCA, HCA, and UpSet analysis, confirmed considerable compositional heterogeneity and demonstrated that both botanical and geographical origins contribute to honey differentiation. Overall, the findings provide new insights into the chemical and botanical characteristics of chestnut, Myosotis, and multifloral honeys from the Eastern Black Sea region and highlight the value of integrated analytical and chemometric approaches for honey characterization, classification, and origin assessment. Full article

The treatment of osteochondral defects still poses a challenge, despite the fact that there are currently many methods and various materials designed for the regeneration of these tissues. The aim of our study was to evaluate the biocompatibility and bioactive potential of an innovative biocement enriched with manuka honey and α-tricalcium phosphate in a porcine model, with a focus on subchondral bone regeneration. Osteochondral defects of the medial femoral condyle were treated with the tested biocement in five animals, and five additional animals served as controls (untreated defect). After six months of observation, the regenerative potential of the therapeutic strategy was evaluated macroscopically, using imaging methods (X-ray, CT, and MRI) and histological examination. The studied biomaterial showed no cytotoxicity and proved to be biocompatible. In the in vivo system, based on imaging and histological methods, a typical trabecular organization with cellular elements has been observed in all animals treated with the tested biocement, indicating active remodeling and maturation processes. In animals with untreated defects, incomplete healing of the bone defect occurred, with limited areas of trabecular bone and incomplete boundaries between the cartilage and subchondral bone. Our pilot study, employing a multi-method approach to investigate the in vivo effects of a novel biocement on bone tissue regeneration, may highlight the potential contribution of biomaterials to the development of new therapeutic strategies in the context of osteochondral regeneration. Full article

Although early-maturing table grapes possess significant commercial value, the metabolic impact of shortened ripening cycles on aroma-quality interactions remains underexplored. This study comprehensively characterized seven cultivars via integrated physicochemical phenotyping (firmness, total soluble solids, and phenolic content) coupled with a multi-platform volatile analysis using E-nose, GC–MS, and GC–IMS. Based on instrumental volatile profiling, the cultivars were classified into three aroma-related groups: a Muscat-type (HY, HMG, JM), enriched in floral monoterpenes; a Strawberry-type (XL, ZML), dominated by fruity, honey-like esters and aldehydes; and a Neutral-type (CG, SB), defined by herbaceous C₆ compounds. Notably, the combined use of E-nose, GC–MS, and GC–IMS provided complementary information for differentiating cultivar-specific volatile profiles. In particular, GC–IMS improved the detection of trace low-molecular-weight volatiles, thereby refining the characterization of cultivar-dependent aroma-related compounds. These findings provide an instrumental basis for understanding volatile differences among early-maturing table grapes and offer useful information for cultivar evaluation, flavor-oriented breeding, and postharvest quality management. Full article

The forecast of fruit weight at harvest requires (i) a non-destructive method for assessment of weight ($F_w$) of fruit-on-tree and (ii) the knowledge of fruit growth dynamics. To address the first issue for mango fruit, several allometric relationships between $F_w$ and fruit-lineal dimensions of length ($L)$, width ($W)$, and thickness ($T)$ were considered, with the relationship $F_w=kLWT$ recommended. A $k$ value of 0.5146 was established for fruit of the cultivar Honey Gold for fruit past the stone-hardening stage, based on assessment of 1091 fruit from 13 season/orchard populations and preliminary values of 0.5376, 0.5151, and 0.5239 for the Keitt, Kensington Pride, and R2E2, respectively, on the basis of more limited datasets. A combined cultivar model was recommended across all cultivars considered, except Keitt. The variation in k between populations of Keitt and Honey Gold fruit was due to the difference in fruit density, rather than shape. This conclusion should be tested in context of other cultivars and fruit development. A correction for developmental age was established for Honey Gold fruit, viz., $k_{corr}=-0.0009\times DAFB+0.5975$ for fruit up to ~1224 growing degree days. The need for similar corrections for other cultivars should be investigated. For the second issue, the use of a linear function based on measurements of $F_w$ in the weeks immediately before harvest was recommended for forecasting harvest-time weight to an accuracy of approximately 10%. A Logistic function described $F_w$ increase better than a Gompertz function; however, a change in growing conditions during fruit development limits the reliability of such models for forecasting fruit weight at harvest maturity. Rather, it is proposed that a set of reference models based on a set of reference Logistic model parameters for a suite of growing conditions be developed for use in guiding agronomic interventions earlier in fruit development to maintain growth on a trajectory to achieve a desired weight at harvest. Full article

Background/Objectives: Managing bone diseases demands novel, natural compounds to bypass the heavy side effects of current therapies. Honey is well-known for its therapeutic traits, yet we know very little about how specific floral varieties impact bone tissue. This study confronts this gap by comparing how acacia, chestnut, and coriander honeys drive human osteoblast behavior in vitro. Methods: After mapping the phenolic/flavonoid profiles and antioxidant capacities of these honeys, we tested them on hFOB 1.19 human osteoblasts. We tracked cell migration via scratch assays and validated osteogenic maturation through Alkaline Phosphatase (ALP) activity and Alizarin Red (AR) mineralization over 7 days. Confocal time-lapse imaging with pharmacological inhibitors monitored intracellular calcium dynamics, while gene shifts were analyzed via qRT-PCR. Results: Coriander honey (CH) packed the highest polyphenol levels and antioxidant power. Biologically, while all honeys accelerated scratch closure, CH drove cell motility most potently. Remarkably, a 7-day treatment with these honeys sparked a significant and robust increase in ALP activity and mineralization, surpassing the osteogenic induction observed with standard osteoinductive media. Mechanistically, CH triggered a sharp [Ca²⁺] spike, relying on external calcium entry and IP3-dependent internal release via PLC activation. qRT-PCR confirmed this anabolic shift via OPG and OPN upregulation. Conclusions: Honey exerts pronounced multi-level osteopromotive effects at both the functional and transcriptional levels, tightly linked to its botanical source. Among the variants, coriander honey stands out for its exceptional ability to fast-track osteoblast migration, differentiation, and early mineral deposition. Therefore coriander honey represents a promising in vitro candidate that warrants further preclinical evaluation for bone repair applications. Full article

Honey maturity is increasingly discussed in relation to product integrity, fair trade, and the classification of immature honey production as a form of adulteration. This narrative critical review examines honey maturity using evidence from peer-reviewed microbiological, physicochemical, and metabolomic studies, combined with an analysis of international and European regulatory frameworks, including Codex Alimentarius CXS 12-1981, Council Directive 2001/110/EC, Regulation (EC) No 178/2002, and Regulation (EC) No 852/2004. Particular attention is given to the interpretation of osmophilic yeast counts, water activity ($a_w$), moisture content, comb cell capping, fermentation, and technological dehumidification. The reviewed evidence indicates that osmophilic yeasts are natural components of honey and that their presence, expressed as colony-forming units per gram (CFU/g), should not be treated as an independent non-compliance criterion in the absence of active fermentation. Existing honey standards define compositional and quality requirements, including moisture, hydroxymethylfurfural, enzymatic activity, and absence of fermentation or effervescence, but do not establish a honey-specific CFU/g limit for yeasts. On this basis, the review formulates a functional maturity assessment framework integrating $a_w$, moisture, enzymatic indicators, and metabolomic biomarkers. The proposed framework is presented as a conceptual model derived from the synthesis of the existing literature and requiring further multilaboratory validation prior to adoption in official control practice. This approach may improve proportionality in honey quality assessment and reduce the risk of misclassifying microbiologically stable honeys as immature or adulterated. Full article

Hops (Humulus lupulus L.) is a plant species with a multitude of uses in medicine, food science and agriculture. Xanthohumol, the major prenylflavonoid in hop cone extract, possesses anti-cancer activity. Xanthohumol also exhibits strong antimicrobial activity against Gram-positive bacteria (e.g., S. aureus), but not against Gram-negative bacteria. Xanthohumol can reduce blood glucose levels and body fat in obese male rats (not females), and mature hop bitter acids (MHBAs) have been found to decrease visceral and abdominal human fat. Xanthohumol can increase bone mineral density, decrease osteoclast numbers, and protect osteoblasts from oxidative stress in osteoporotic mice. Further clinical research, xanthohumol and bitter acids could be sourced from hop cone extracts to formulate novel drugs that can successfully treat a variety of diseases and potentially replace current therapies that have negative effects. In the food industry, hop cone extracts are mainly used in the brewing industry, with 98% of the world’s hop cones being used in brewing beer. Hop cone extracts are also used as food/drink preservatives due to their antimicrobial abilities, as previously mentioned, although there is less of a need for hops in extending food/drink shelf-life. Finally, hop cone extracts have several uses in agriculture, mainly as pesticides. For example, hop extracts can kill varroa mites, a parasite that impairs honeybee health. This benefits honeybee farmers as increased bee survival means more honey production, increasing profits. Overall, this review paper brings together recent studies that highlight hop extracts as valuable bioactive compound mixtures with many useful applications. Full article

Traceability systems are increasingly central to ensure food safety, quality, biosecurity, and sustainability in agrifood supply chains. Despite advances in digital technologies, adoption and effective implementation remain uneven, with many producers still relying on paper-based systems. This study examines the motivations and conditions that enable or constrain the participation in traceability systems by Australian red meat and honey producers using the Digital Maturity Framework (DMF) as a diagnostic lens. Drawing on seven focus groups and five individual interviews involving a total of 73 producers and supply chain stakeholders from both sectors, the study investigates how value perceptions, technology and infrastructure, data analytics and management, capability, and data governance, influence producers’ engagement with traceability systems. Our findings indicate that while regulatory pressure and market opportunities incentivise adoption, several challenges persist, including high costs, limited digital skills, data sharing concerns, and sector-specific constraints. The red meat sector demonstrates higher digital maturity, driven largely by compliance mandates and an established regulatory system. In contrast, the honey bee sector exhibits more fragmented traceability adoption, challenged by the predominance of small-scale producers and limited trust in data sharing mechanisms. The comparison between two sectors reveals the influence of sectoral context. In particular, the regulatory frameworks and supply chain coordination play a relevant role in the adoption of traceability technologies. Overall, this research reveals the need for tailored policy and industry support, including regulatory harmonisation, improved data interoperability, digital infrastructure, and capacity-building initiatives to enable more consistent and broader traceability implementation across agrifood systems. Full article

Behavioral maturation is essential for the proper functioning of honey bee societies and is regulated by multiple factors such as juvenile hormone (JH) and nutritional deficiency. Although recent studies have shown that surface-associated microbiota in insects can modulate host behavior, the relationship between body surface microbiota and behavioral maturation in honey bees remains largely unexplored. This study aimed to determine whether the surface microbial communities of honey bees shift with behavioral maturation. By using 16S rRNA gene amplicon sequencing, we analyzed the surface microbiota of worker bees at different behavioral stages (newly emerged bees, nurses, and foragers) in both Eastern honey bee Apis cerana and Western honey bee Apis mellifera. The results showed that in both honey bee species, nurse bees exhibited the lowest microbial diversity, while forager bees showed the highest, and newly emerged bees had an intermediate level of microbial diversity. Moreover, beta diversity analyses revealed that the body surface microbiota of worker bees significantly varied across behavioral stages in both bee species and differed between the two bee species at the same behavioral stage. Additionally, in both bee species, at the phylum level, Pseudomonadota, Bacillota, and Actinobacteriota dominated the worker bee body surface microbiota; at the genus level, foragers had more Gilliamella, while nurses harbored more Lactobacillus. Together, our findings reveal the emergence of distinct microbial signatures on honey bee body surfaces during behavioral maturation. Full article

‘Hainan Qingyou’ (Citrus maxima) Pomelo is one of the predominant local cultivars cultivated in Hainan Province, renowned for its high economic value and strong market competitiveness. However, during cultivation, it was observed that the fruit quality of ‘Hainan Qingyou’ grafted onto a ‘Sanhong’ interstock deteriorated, predominantly manifesting as vesicle granulation. This study was therefore conducted to investigate this phenomenon using ‘Sanhong’ Honey Pomelo as the interstock. Fruit quality indicators were measured, and pulp transcriptomic analysis was performed during the expansion and maturation stages. The results indicated that fruits grafted onto ‘Sanhong’ interstock (SHZ) exhibited increased peel thickness, yellower peel, reduced edible rate, higher pulp firmness, decreased total soluble solids (TSS), increased total acid content, and reduced total antioxidant capacity at maturity, all contributing to diminished fruit quality. Additionally, SHZ fruit accumulated higher lignin content in the pulp, leading to vesicle granulation, which severely compromised marketability. Transcriptomic analysis identified 42 structural genes involved in lignin biosynthesis in ‘Hainan Qingyou’ pulp, including 5 PAL, 2 C4H, 2 4CL, 6 CAD, 15 PER, 2 HCT, 1 C3′H, 1 CCoAOMT, 1 CCR, 1 COMT, 2 CSE, and 1 F5H genes. Most of these genes were highly expressed in SHZ fruit at maturity, with expression levels significantly higher than those in fruit grafted onto ‘Hainan Qingyou’ interstock (QYZ). The interstock also affected hormone signaling pathways. Weighted gene co-expression network analysis (WGCNA) identified transcription factors such as MYB, MIKC, ERF, and bZIP as key regulators involved in pulp lignin biosynthesis. This study provides insights into the effects of rootstocks on citrus fruit quality and offers valuable information for cultivar improvement in pomelo orchards. Full article

Honey quality and authenticity are influenced by floral origin, processing, and storage, with implications for composition and sensory appeal. This study offers a comparative assessment of eight monofloral honey samples, representing five botanical varieties: acacia, linden, rapeseed, lavender, and thyme. For acacia, linden, and rapeseed, both producer-sourced and commercial honeys were analyzed, while lavender and thyme samples were available only from local beekeepers. The botanical origin of each sample was confirmed using morphological markers of pollen grains. Physicochemical characterization included acidity, pH, moisture content, refractive index, hydroxymethyl furfural (HMF), proline concentration, and carbohydrate profiling by HPLC-RID. Acacia honey exhibited the lowest acidity and HMF levels, alongside the highest fructose/glucose (F/G) ratios, indicating superior freshness, lower crystallization risk, and a sweeter flavor profile. In contrast, rapeseed honey showed elevated glucose levels and the lowest F/G ratio, confirming its tendency to crystallize rapidly. All samples recorded proline concentrations well above the quality threshold (180 mg/kg), supporting their authenticity and proper maturation. The estimated glycemic index (eGI) varied between 43.91 and 62.68 and was strongly inversely correlated with the F/G ratio (r = −0.98, p < 0.001). Sensory evaluation highlighted acacia honey from producers as the most appreciated across visual, tactile, and flavor attributes. Correlation analyses further revealed consistent links between sugar composition and both physical and sensory properties. Overall, the findings reinforce the value of integrated analytical and sensory profiling in assessing honey quality and authenticity. Full article

Honey bees are one of the most significant pollinators and contribute to the pollination of various crops. The honey bee, Apis mellifera (Linnaeus, 1758), has unique characteristics that could be successfully used to improve biomonitoring approaches in assessing environmental interactions. Three apiaries with different rates of honey bee colony losses were included in the study—Dimovtsi, Plovdiv, and Krasnovo, Bulgaria. Male individuals (immature and mature) were collected from five colonies for each of the three apiaries and studied for histopathological changes in the gonads. The results concerning the rate of honey bee colony losses in the studied apiaries from 2022 and 2023 showed honey bee losses in the tested colonies due to queen problems, which were reported for Plovdiv, as well as the death of honey bees or a reduction in their number to a few hundred bees in the colony. The chemical analysis showed the presence of different organic substances, such as Coumaphos, DEET (N, N-diethyl-M-toluamide), Fluvalinate, and Piperonyl-butoxide, in the alive and dead honey bee samples and those of food stocks (wax, pollen, and honey) within the hives. Among the sample types, those of the dead honey bees contained the greatest variety of pesticide residues, particularly in Plovdiv and Dimovtsi, reinforcing the link between pesticide exposure and honey bee mortality. The histopathological alterations were mainly associated with the thinning of the covering epithelium of the seminiferous tubules and the detachment of the basement membrane of the seminiferous tubules. The more severe histopathological lesion, necrosis, was observed in a higher degree of expression in the drones from Plovdiv, indicating a higher pollution level in this region. Full article

Aprostocetus nitens Prinsloo & Kelly (Hymenoptera: Eulophidae) was identified as one of four hymenopteran ectoparasitoids utilizing three erythrina gall wasps, Quadrastichus bardus, Q. erythrinae, and Q. gallicola) (Hymenoptera: Eulophidae) in the native eastern Africa. In Hawaiʻi, the eurytomid wasp, Eurytoma erythrinae Gates & Delvare (Hymenoptera: Eurytomidae), was introduced and approved for statewide release in 2008 to control the erythrina gall wasp (EGW) Q. erythrinae Kim. EGW has devastated the wiliwili trees, Erythrina sandwicensis Degener (Fabaceae), an ecologically and culturally important native Hawaiian tree species. However, the parasitoid’s impact on the galled inflorescences and shoots was not adequate to ensure adequate seed set and maturation for successful tree recruitment. Aprostocetus nitens was thus evaluated as a prospective natural enemy to enhance the biological control of EGW to further protect the wiliwili trees in Hawaiʻi. Both choice and no-choice host specificity tests were conducted on seven non-target gall formers in the Hawaii Department of Agriculture, Insect Containment Facility, and showed that the parasitoid was extremely specific to EGW. The potential for competition between this parasitoid and the established E. erythrinae was also investigated, showing that the release of a second parasitoid will potentially complement the success of the eurytomid wasp for control of EGW. Unlike what was found in the native region, the Hawaiian laboratory colony is thelytokous, producing only female offspring. The life cycle took 20.1 ± 0.28 days under the laboratory conditions. Non-ovipositing female survived for 102.5 ± 2.9 days when fed honey and laid eggs for 25.1 ± 2.3 days with average fecundity of 156.7 ± 22.3 offspring/female. This value is 3.9-fold higher than offspring produced by E. erythrinae. Aprostocetus nitens, host specificity, competition with E. erythrinae, and its tri-trophic association with 15 Erythrina host plants and 5 gall wasp assemblages in the native African regions were defined. Implications to reduce frequent galls on the native Erythrina plants and likely domination over E. erythrinae, are discussed. Full article

This article examines the key factors influencing the aromatic profile of mead, which is increasingly popular in artisanal markets worldwide. Based on a bibliometric review of 44 scientific studies, the analysis highlights the significant role of honey type in shaping mead’s sensory characteristics. Acacia honey contributes subtle floral notes, while eucalyptus honey brings bolder, resinous aromas. The bibliometric analysis also emphasizes fermentation conditions, such as temperature and yeast selection, as crucial factors. Lower fermentation temperatures help preserve volatile compounds, enhancing fruity and floral aromas, while higher temperatures lead to increased concentrations of undesirable higher alcohols. Additionally, aging mead in oak barrels for 6 to 12 months adds complexity by introducing vanilla, coconut, and spice notes from the wood’s phenolic compounds. The maturation process, including its duration and storage conditions, also enables the flavors to blend and develop over time. Moreover, the addition of herbs and fruits during fermentation or maturation has been proven to introduce new layers of aroma and flavor, with ingredients like citrus, berries, and aromatic herbs enhancing the final product with fresh, lively notes. The potential of non-Saccharomyces yeasts is also explored as an alternative for enriching aromatic profiles, with the capacity to introduce unique sensory characteristics, including diverse flavor profiles and regional or terroir-based variations. Finally, the bibliometric review reinforces the importance of selecting appropriate ingredients and controlling fermentation processes to improve mead quality. It also suggests exploring microbiomes, exotic honey varieties, and the use of herbs and fruits for even more distinct aromatic profiles. Full article

Background: Flowering members of the globally diffused Rosaceae family include popular plants, such as apple, almond, and cherry, which play a fundamental role as honeybee nectariferous and polleniferous agents. Through the production of honey, these plants can also play an indirect role in the prevention and treatment of many diseases, including infections, fighting the occurrence of resistant microorganisms, and concurrently stimulating the growth of beneficial bacteria. Objectives: This study focused on the effect of some Rosaceae plants’ honey, including hawthorn, cherry, raspberry, almond, and apple, against the pathogens Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Listeria monocytogenes, Pseudomonas aeruginosa, and Staphylococcus aureus. Results: Results demonstrated the honey’s ability to impair swimming motility. A crystal violet test indicated that honey could inhibit the formation and stabilization of biofilms, with inhibition rates up to 59.43% for immature biofilms (showed by apple honey against A. baumannii) and 39.95% for sessile bacterial cells in mature biofilms (when we used cherry honey against S. aureus). In the test with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, cherry and apple honey were the most effective in inhibiting sessile cell metabolism honey in both immature (56.47% cherry honey vs. K. pneumoniae) and mature biofilms (54.36% apple honey vs. A. baumannii). Honey stimulated the growth of Lactobacillus bulgaricus, Lacticaseibacillus casei Shirota, Lactobacillus gasseri, Lacticaseibacillus plantarum, and Lacticaseibacillus rhamnosus; hawthorn, raspberry, and almond honey significantly increased the in vitro adhesion capacity of L. bulgaricus and L. casei Shirota. Tests with probiotic supernatants demonstrated honey’s ability to inhibit the biofilm formation and metabolism of the pathogens. Conclusions: Our results encourage further studies to assess the potential application of Rosaceae honey for food preservation and in the health field, as it could fight the antimicrobial resistance of food and clinical pathogens, and potentially enhance the host’s gut wellness. The use of honey for nanotechnological and biotechnological approaches could be suggested too. Full article