Search Results (536)

The Xiong’an New Area, a newly established national-level zone in China, faces the threat of land subsidence and ground fissure due to groundwater overexploitation and geothermal extraction, threatening urban safety. This study integrates time-series InSAR and GNSS monitoring to analyze spatiotemporal deformation patterns from 2017/05 to 2025/03. The key results show: (1) Three subsidence hotspots, namely northern Xiongxian (max. cumulative subsidence: 591 mm; 70 mm/yr), Luzhuang, and Liulizhuang, strongly correlate with geothermal wells and F4/F5 fault zones; (2) GNSS baseline analysis (e.g., XA01-XA02) reveals fissure-induced differential deformation (max. horizontal/vertical rates: 40.04 mm/yr and 19.8 mm/yr); and (3) InSAR–GNSS cross-validation confirms the high consistency of the results (Pearson’s correlation coefficient = 0.86). Subsidence in Xiongxian is driven by geothermal/industrial groundwater use, without any seasonal variations, while Anxin exhibits agricultural pumping-linked seasonal fluctuations. The use of rooftop GNSS stations reduces multipath effects and improves urban monitoring accuracy. The spatiotemporal heterogeneity stems from coupled resource exploitation and tectonic activity. We propose prioritizing rooftop GNSS deployments to enhance east–west deformation monitoring. This framework balances regional and local-scale precision, offering a replicable solution for geological risk assessments in emerging cities. Full article

Artificial intelligence (AI) techniques—ranging from hybrid mechanistic–machine learning (ML) ensembles to gradient-boosted decision trees, support-vector machines, and deep neural networks—are transforming the management of seasonal influenza, respiratory syncytial virus (RSV), human immunodeficiency virus (HIV), and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Symptom-based triage models using eXtreme Gradient Boosting (XGBoost) and Random Forests, as well as imaging classifiers built on convolutional neural networks (CNNs), have improved diagnostic accuracy across respiratory infections. Transformer-based architectures and social media surveillance pipelines have enabled real-time monitoring of COVID-19. In HIV research, support-vector machines (SVMs), logistic regression, and deep neural network (DNN) frameworks advance viral-protein classification and drug-resistance mapping, accelerating antiviral and vaccine discovery. Despite these successes, persistent challenges remain—data heterogeneity, limited model interpretability, hallucinations in large language models (LLMs), and infrastructure gaps in low-resource settings. We recommend standardized open-access data pipelines and integration of explainable-AI methodologies to ensure safe, equitable deployment of AI-driven interventions in future viral-outbreak responses. Full article

In recent years, the persistent emergence of novel infectious pathogens (epitomized by the global coronavirus disease-2019 (COVID-2019) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)) has propelled nucleic acid testing (NAT) into an unprecedented phase of rapid development. As a key technology in modern molecular diagnostics, NAT achieves precise pathogen identification through specific nucleic acid sequence recognition, establishing itself as an indispensable diagnostic tool across diverse scenarios, including public health surveillance, clinical decision-making, and food safety control. The reliability of NAT systems fundamentally depends on reference materials (RMs) that authentically mimic the biological characteristics of natural viruses. This critical requirement reveals significant limitations of current RMs in the NAT area: naked nucleic acids lack the structural authenticity of viral particles and exhibit restricted applicability due to stability deficiencies, while inactivated viruses have biosafety risks and inter-batch heterogeneity. Notably, pseudovirus has emerged as a novel RM that integrates non-replicative viral vectors with target nucleic acid sequences. Demonstrating superior performance in mimicking authentic viral structure, biosafety, and stability compared to conventional RMs, the pseudovirus has garnered substantial attention. In this comprehensive review, we critically summarize the engineering strategies of pseudovirus platforms and their emerging role in ensuring the reliability of NAT systems. We also discuss future prospects for standardized pseudovirus RMs, addressing key challenges in scalability, stability, and clinical validation, aiming to provide guidance for optimizing pseudovirus design and practical implementation, thereby facilitating the continuous improvement and innovation of NAT technologies. Full article

Background: Understanding the duration and quality of immune memory following SARS-CoV-2 infection and vaccination is critical for informing public health strategies and vaccine development. While waning antibody levels have raised concerns about long-term protection, the persistence of memory B cells (MBCs) and T cells plays a vital role in sustaining immunity. Materials and Methods: We conducted a longitudinal prospective study over 12 months, enrolling 285 participants in total, either after natural infection or vaccination with BNT162b2 or mRNA-1273. Peripheral blood samples were collected at four defined time points (baseline, 1–2 months, 6–7 months, and 12–13 months after vaccination or disease onset). Immune responses were assessed through serological assays quantifying anti-RBD IgG and neutralizing antibodies, B-ELISPOT, and multiparameter flow cytometry for S1-specific memory B cells. Results: Both mRNA vaccines induced robust B cell and antibody responses, exceeding those observed after natural infection. Memory B cell frequencies peaked at 6 months and declined by 12 months, but remained above the baseline. The mRNA-1273 vaccine elicited stronger and more durable humoral and memory B-cell-mediated immunity compared to BNT162b2, likely influenced by its higher mRNA dose and longer prime-boost interval. Class-switched memory B cells and S1-specific B cells were significantly expanded in vaccine recipients. Natural infection induced more heterogeneous immune memory. Conclusions: Both mRNA vaccination and natural SARS-CoV-2 infection induce a comparable expansion of memory B cell subsets, reflecting a consistent pattern of humoral immune responses across all studied groups. These findings highlight the importance of vaccination in generating sustained immunological memory and suggest that the vaccine platform and dosage influence the magnitude and durability of immune responses against SARS-CoV-2. Full article

Background/Objectives: SARS-CoV-2 vaccine candidates comprising the receptor binding domain (RBD) of the spike protein have been shown to confer protection against infection. Previous research evaluating vaccine candidates with SARS-CoV-2 RBD fused to ferritin (RBD-ferritin) and other scaffolds suggested that multimeric assemblies of RBD can enhance antigen presentation to improve the potency and breadth of immune responses. Though RBDs directly fused to a self-assembling scaffold can be delivered as messenger RNA (mRNA) formulated with lipid nanoparticles (LNPs), reports of SARS-CoV-2 vaccine candidates that combine these approaches remain scarce. Methods: Here, we designed RBD fused to AP205 or TIP60 self-assembling nanoparticles following a search of available structures focused on several scaffold properties. RBD-AP205 and RBD-TIP60 were tested for antigenicity following transfection and for immunogenicity and neutralization potency when delivered as mRNA in mice, with RBD-ferritin as a direct comparator. Results: All scaffolded RBD constructs were readily secreted to transfection supernatant and showed antigenicity in ELISA, though clear heterogeneity in assembly was observed. RBD-AP205 and RBD-TIP60 also exhibited robust antibody binding and neutralization titers in mice that were comparable to those elicited by RBD-ferritin or a full-length membrane-bound spike. Conclusions: These data suggest that AP205 and TIP60 can present RBD as effectively as ferritin and induce similar immune responses. By describing additional scaffolds for multimeric display that accommodate mRNA delivery platforms, this work can provide new tools for future vaccine design efforts. Full article

Image registration constitutes the fundamental basis for the joint interpretation of synthetic aperture radar (SAR) and optical images. However, robust image registration remains challenging due to significant regional heterogeneity in remote sensing scenes (e.g., co-existing urban and marine areas within a single image). To overcome this challenge, this article proposes a novel optical–SAR image registration method named Gradient and Standard Deviation Feature Weighted Fusion (GDWF). First, a Block-local standard deviation (Block-LSD) operator is proposed to extract block-based feature points with regional adaptability. Subsequently, a dual-modal feature description is developed, constructing both gradient-based descriptors and local standard deviation (LSD) descriptors for the neighborhoods surrounding the detected feature points. To further enhance matching robustness, a confidence-weighted feature fusion strategy is proposed. By establishing a reliability evaluation model for similarity measurement maps, the contribution weights of gradient features and LSD features are dynamically optimized, ensuring adaptive performance under varying conditions. To verify the effectiveness of the method, different optical and SAR datasets are used to compare it with the currently advanced algorithms MOGF, CFOG, and FED-HOPC. The experimental results demonstrate that the proposed GDWF algorithm achieves the best performance in terms of registration accuracy and robustness among all compared methods, effectively handling optical–SAR image pairs with significant regional heterogeneity. Full article

Forest biomass is closely related to carbon sequestration capacity and can reflect the level of forest management. This study utilizes four machine learning algorithms, namely Multivariate Stepwise Regression (MSR), K-Nearest Neighbors (k-NN), Artificial Neural Network (ANN), and Random Forest (RF), to estimate forest aboveground biomass (AGB) in Chenzhou City, Hunan Province, China. In addition, a canopy height model, constructed from a digital surface model (DSM) derived from Sentinel-1 Interferometric Synthetic Aperture Radar (InSAR) and an ICESat-2-corrected SRTM DEM, is incorporated to quantify its impact on the accuracy of AGB estimation. The results indicate the following: (1) The incorporation of multi-source remote sensing data significantly improves the accuracy of AGB estimation, among which the RF model performs the best (R² = 0.69, RMSE = 24.26 t·ha⁻¹) compared with the single-source model. (2) The canopy height model (CHM) obtained from InSAR-LiDAR effectively alleviates the signal saturation effect of optical and SAR data in high-biomass areas (>200 t·ha⁻¹). When FCH is added to the RF model combined with multi-source remote sensing data, the R² of the AGB estimation model is improved to 0.74. (3) In 2018, AGB in Chenzhou City shows clear spatial heterogeneity, with a mean of 51.87 t·ha⁻¹. Biomass increases from the western hilly part (32.15–68.43 t·ha⁻¹) to the eastern mountainous area (89.72–256.41 t·ha⁻¹), peaking in Dongjiang Lake National Forest Park (256.41 t·ha⁻¹). This study proposes a comprehensive feature integration framework that combines red-edge spectral indices for capturing vegetation physiological status, SAR-derived texture metrics for assessing canopy structural heterogeneity, and canopy height metrics to characterize forest three-dimensional structure. This integrated approach enables the robust and accurate monitoring of carbon storage in subtropical forests. Full article

Snow water equivalent (SWE) can be estimated using Differential SAR Interferometry (DInSAR), which captures changes in snow depth and density between two SAR acquisitions. However, challenges arise due to SAR signal penetration into the snowpack and the intrinsic limitations of DInSAR measurements. This study addresses these issues and explores the use of multi-band SAR data to derive SWE maps in alpine regions characterized by steep terrain, small spatial extent, and a potentially heterogeneous snowpack. We first conducted a performance analysis to assess SWE estimation precision and the maximum unambiguous SWE variation, considering incidence angle, wavelength, and coherence. Based on these results, we selected C-band Sentinel-1 and L-band SAOCOM data acquired over alpine areas and applied tailored DInSAR processing. Atmospheric artifacts were corrected using zenith total delay maps from the GACOS service. Additionally, sensitivity maps were generated for each interferometric pair to identify pixels suitable for reliable SWE estimation. A comparative analysis of the C- and L-band results revealed several critical issues, including significant atmospheric artifacts, phase decorrelation, and phase unwrapping errors, which impact SWE retrieval accuracy. A comparison between our Sentinel-1-based SWE estimations and independent measurements over an instrumented site shows results fairly in line with previous works exploiting C-band data, with an RSME in the order of a few tens of mm. Full article

Background/Objectives: Epidemiological modeling is a vital tool for managing pandemics, including SARS-CoV-2. Advances in the understanding of epidemiological dynamics and access to new data sources necessitate ongoing adjustments to modeling techniques. In this study, we present a significantly expanded and updated version of our previous SARS-CoV-2 model formulated as input–output non-linear dynamical systems (IO-NLDS). Methods: This updated framework incorporates age-dependent contact patterns, immune waning, and new data sources, including seropositivity studies, hospital dynamics, variant trends, the effects of non-pharmaceutical interventions, and the dynamics of vaccination campaigns. Results: We analyze the dynamics of various datasets spanning the entire pandemic in Germany and its 16 federal states using this model. This analysis enables us to explore the regional heterogeneity of model parameters across Germany for the first time. We enhance our estimation methodology by introducing constraints on parameter variation among federal states to achieve this. This enables us to reliably estimate thousands of parameters based on hundreds of thousands of data points. Conclusions: Our approach is adaptable to other epidemic scenarios and even different domains, contributing to broader pandemic preparedness efforts. Full article

Sleep deprivation impairs immune function, and melatonin has emerged as a key mediator in this process. This narrative review analyzes 50 studies published between 2000 and 2025 to determine the extent to which reduced melatonin synthesis contributes to immune dysregulation. Consistent sleep loss lowers melatonin levels, which correlates with elevated proinflammatory cytokines (e.g., IL-6 and TNF-α), increased oxidative stress, and reduced immune cell activity, including that of natural killer (NK) cells and CD4+ lymphocytes. Melatonin regulates immune pathways, including NF-κB signaling. It also supports mitochondrial health and helps maintain gut barrier integrity. These effects are particularly relevant in vulnerable populations, including older adults and shift workers. Experimental findings also highlight melatonin’s therapeutic potential in infections like SARS-CoV-2, where it modulates inflammatory responses and viral entry mechanisms. Despite the heterogeneity of study methodologies, a consistent correlation emerges between circadian disruption, melatonin suppression, and immune imbalance. These findings underscore melatonin’s dual role as a chronobiotic and immunomodulator. Addressing sleep loss and considering melatonin-based interventions may help restore immune homeostasis. More clinical trials are needed to determine the best dosing, long-term efficacy, and population-specific strategies for supplementation. Promoting healthy sleep is crucial for preventing chronic inflammation and diseases associated with immune dysfunction. Full article

Objectives: The durability and breadth of neutralizing antibodies following SARS-CoV-2 mRNA vaccination remain incompletely understood. This study aimed to investigate how longitudinal changes in antibody isotype composition impact neutralization against structurally diverse SARS-CoV-2 variants. Methods: After screening a broader cohort of mRNA-vaccinated sera, time-matched samples collected one month (1 mpv) and three months post-vaccination (3 mpv) were selected for detailed analysis. Neutralization assays against live virus variants, enzyme-linked immunosorbent assays (ELISA), and immunogold electron microscopy were performed to assess antibody titers, isotype levels, and virion morphology. Results: Neutralization titers declined markedly at 3 mpv, particularly against immune-evasive variants. Notably, the Lambda variant showed disproportionately high sensitivity to early-phase sera despite its divergence from the vaccine strain. Antibody isotyping showed that IgA and IgM decreased over time, while IgG levels were relatively more sustained. Electron microscopy revealed broader virion size heterogeneity in Lambda (50–200 nm) compared to Wuhan (80–120 nm), potentially enhancing multivalent antibody engagement. Consistently, ELISA under reduced spike density conditions showed that IgA and IgM retained stronger binding than IgG. Conclusions: These findings indicate that the decline of IgA and IgM compromises neutralization breadth, especially against structurally divergent variants such as Lambda. Sustaining dynamic multivalent isotype responses that adapt to diverse spike morphologies may be critical for broad cross-variant immunity. Full article

Debris flows pose significant risks to mountainous regions, and quick, accurate volume estimation is crucial for hazard assessment and post-disaster response. Traditional volume estimation methods, such as ground surveys and aerial photogrammetry, are often limited by cost, accessibility, and timeliness. While remote sensing offers wide coverage, existing optical and Synthetic Aperture Radar (SAR)-based techniques face challenges in direct volume estimation due to resolution constraints and rapid terrain changes. This study proposes a Super-Resolution Shape from Shading (SRSFS) approach enhanced by a Non-local Piecewise-smooth albedo Constraint (NPC), hereafter referred to as NPC SRSFS, to estimate debris-flow erosion volume using single high-resolution optical satellite imagery. By integrating publicly available global Digital Elevation Model (DEM) data as prior terrain reference, the method enables accurate post-disaster topography reconstruction from a single optical image, thereby reducing reliance on stereo imagery. The NPC constraint improves the robustness of albedo estimation under heterogeneous surface conditions, enhancing depth recovery accuracy. The methodology is evaluated using Gaofen-6 satellite imagery, with quantitative comparisons to aerial Light Detection and Ranging (LiDAR) data. Results show that the proposed method achieves reliable terrain reconstruction and erosion volume estimates, with accuracy comparable to airborne LiDAR. This study demonstrates the potential of NPC SRSFS as a rapid, cost-effective alternative for post-disaster debris-flow assessment. Full article

Accurate calibration and validation of remote sensing soil moisture products critically depend on high-quality in situ measurements. However, effectively capturing representative soil moisture patterns across heterogeneous catchments using ground-based sensors remains a significant challenge. To address this, we propose a machine-learning-based framework for optimizing soil moisture sensor network deployment at the catchment scale. The framework was validated using Sentinel-1 SAR-derived soil moisture data within a humid catchment in southern China. Results show that a network of nine optimally placed sensors minimized prediction errors (RMSE: 7.20%), outperforming both sparser and denser configurations. The optimized sensor network achieved a 52.45% reduction in RMSE compared to random placement. Moreover, the optimal number of sensors varied with seasonal dynamics: the wet season required 11 sensors due to increased precipitation-induced spatial variability, whereas the dry season could be adequately monitored with only six sensors. The proposed optimization approach offers a cost-effective strategy for collecting reliable in situ data, which is essential for improving the accuracy and applicability of remote sensing products in catchment-scale soil moisture monitoring. Full article

High thematic resolution vegetation mapping is essential for monitoring wetland ecosystems, supporting conservation, and guiding water management. However, producing accurate, fine-scale vegetation maps in large, heterogeneous floodplain wetlands remains challenging due to complex hydrology, spectral similarity among vegetation types, and the high cost of extensive field surveys. This study addresses these challenges by developing a scalable vegetation classification framework that integrates cluster-guided sample selection, Random Forest modelling, and multi-source remote-sensing data. The approach combines multi-temporal Sentinel-1 SAR, Sentinel-2 optical imagery, and hydro-morphological predictors derived from LiDAR and hydrologically enforced SRTM DEMs. Applied to the Great Cumbung Swamp, a structurally and hydrologically complex terminal wetland in the lower Lachlan River floodplain of Australia, the framework produced vegetation maps at three hierarchical levels: formations (9 classes), functional groups (14 classes), and plant community types (PCTs; 23 classes). The PCT-level classification achieved an overall accuracy of 93.2%, a kappa coefficient of 0.91, and a Matthews correlation coefficient (MCC) of 0.89, with broader classification levels exceeding 95% accuracy. These results demonstrate that, through targeted sample selection and integration of spectral, structural, and terrain-derived data, high-accuracy, high-resolution wetland vegetation mapping is achievable with reduced field data requirements. The hierarchical structure further enables broader vegetation categories to be efficiently derived from detailed PCT outputs, providing a practical, transferable tool for wetland monitoring, habitat assessment, and conservation planning. Full article

Current synthetic aperture radar (SAR) ship datasets exhibit a notable disparity in the distribution of large, medium, and small ship targets. This imbalance makes it difficult for a relatively small number of large and medium-sized ships to be effectively trained, resulting in many false alarms. Therefore, to address the issues of scale diversity, intra-class imbalance in ship data, and the feature conflict problem associated with traditional coupled detection heads, we propose an SAR image multiscale task-decoupled oriented ship target detector based on a size-aware balanced strategy. First, the multiscale target features are extracted using the multikernel heterogeneous perception module (MKHP). Meanwhile, the triple-attention module is introduced to establish the remote channel dependence to alleviate the issue of small target feature annihilation, which can effectively enhance the feature characterization ability of the model. Second, given the differences in the demand for feature information between the detection and classification tasks, a channel attention-based task decoupling dual-head (CAT2D) detector head structure is introduced to address the inherent conflict between classification and localization tasks. Finally, a new size-aware balanced (SAB) loss strategy is proposed to guide the network in focusing on the scarce targets in training to alleviate the intra-class imbalance problem during the training process. The ablation experiments on SSDD+ reflect the contribution of each component, and the results of the comparison experiments on the RSDD-SAR and HRSID datasets show that the proposed method achieves state-of-the-art performance compared to other state-of-the-art detection models. Furthermore, our approach exhibits superior detection coverage for both offshore and inshore scenarios for ship detection tasks. Full article