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Molecular Research on Virus-Related Infectious Disease

A special issue of Current Issues in Molecular Biology (ISSN 1467-3045). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 30 September 2025 | Viewed by 676

Special Issue Editors


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Guest Editor
Department of Epidemiology, School of Public Health, Zhengzhou University, Zhengzhou 450001, China
Interests: molecular epidemiology; genetic engineering; vaccines; molecular biology; CRISPR

E-Mail Website
Guest Editor
Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China
Interests: epidemiology; drug resistance; oncology; infectious diseases; vaccines
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Viruses have a global impact on healthcare and on social and economic development because they are the largest cause of mortality due to infectious disease, such as flu, SARS-CoV-2, monkeypox, etc. Investigations into virus-related infectious diseases encompassing molecular epidemiology and molecular etiology play a pivotal role in elucidating viral transmission patterns, epidemic models, as well as intricate host–virus interactions.

In recent years, there has been rapid advancement in molecular research on virus-related infectious diseases; however, continuous exploration of novel techniques and ideas remains imperative, which aims to further enhance our comprehension of viral mechanisms and pave the way for innovative approaches towards prevention, diagnosis, and treatment of viral infections.

This Special Issue is intended to cover all the past and ongoing efforts on molecular research on virus-related infectious disease. In this Special Issue, original research articles and reviews are welcome. Research areas may include (but are not limited to) the following:

  • Molecular diagnosis of viruses;
  • Viral genomics;
  • Host–virus interactions;
  • Molecular pathways;
  • Therapeutic targets;
  • Vaccine development.

Prof. Dr. Guangcai Duan
Dr. Shuaiyin Chen
Guest Editors

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • molecular diagnosis of viruses
  • viral genomics
  • host–virus interactions
  • molecular pathways
  • therapeutic targets
  • vaccine development

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Published Papers (1 paper)

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Review

15 pages, 501 KiB  
Review
Pseudovirus as an Emerging Reference Material in Molecular Diagnostics: Advancement and Perspective
by Leiqi Zheng and Sihong Xu
Curr. Issues Mol. Biol. 2025, 47(8), 596; https://doi.org/10.3390/cimb47080596 - 29 Jul 2025
Viewed by 333
Abstract
In recent years, the persistent emergence of novel infectious pathogens (epitomized by the global coronavirus disease-2019 (COVID-2019) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)) has propelled nucleic acid testing (NAT) into an unprecedented phase of rapid development. As a key [...] Read more.
In recent years, the persistent emergence of novel infectious pathogens (epitomized by the global coronavirus disease-2019 (COVID-2019) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)) has propelled nucleic acid testing (NAT) into an unprecedented phase of rapid development. As a key technology in modern molecular diagnostics, NAT achieves precise pathogen identification through specific nucleic acid sequence recognition, establishing itself as an indispensable diagnostic tool across diverse scenarios, including public health surveillance, clinical decision-making, and food safety control. The reliability of NAT systems fundamentally depends on reference materials (RMs) that authentically mimic the biological characteristics of natural viruses. This critical requirement reveals significant limitations of current RMs in the NAT area: naked nucleic acids lack the structural authenticity of viral particles and exhibit restricted applicability due to stability deficiencies, while inactivated viruses have biosafety risks and inter-batch heterogeneity. Notably, pseudovirus has emerged as a novel RM that integrates non-replicative viral vectors with target nucleic acid sequences. Demonstrating superior performance in mimicking authentic viral structure, biosafety, and stability compared to conventional RMs, the pseudovirus has garnered substantial attention. In this comprehensive review, we critically summarize the engineering strategies of pseudovirus platforms and their emerging role in ensuring the reliability of NAT systems. We also discuss future prospects for standardized pseudovirus RMs, addressing key challenges in scalability, stability, and clinical validation, aiming to provide guidance for optimizing pseudovirus design and practical implementation, thereby facilitating the continuous improvement and innovation of NAT technologies. Full article
(This article belongs to the Special Issue Molecular Research on Virus-Related Infectious Disease)
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