Search Results (932)

Pseudomonas has been revealed as an important member of plant probiotics, with its rich species diversity implying complementary plant growth-promoting (PGP) traits. However, information on Pseudomonas species in the microecology of Angelica sinensis and medicinal plants in general remains to be further investigated. This study examined the microecological characteristics, PGP traits, and their underlying molecular mechanisms of Pseudomonas. Filling this gap will provide an important reference for microbial community design centered on dominant functional bacterial genera. In this study, we characterized the microecological traits, PGP properties, and their underlying molecular mechanisms of Pseudomonas strains. Microbiome analysis identified Pseudomonas as the dominant genus in the rhizosphere and a core endophytic genus, exerting significant influences on both (path coefficients = 0.971, 0.872). Comparative phenomics suggested potential functional complementarity among different strains. Our observations revealed significant differentiation in PGP traits: P. umsongensis X08 showed exceptional performance in IAA and siderophore production (IAA: 1.24 mg/mL, siderophore halo diameter: 2.04 cm); P. frederiksbergensis X06 exhibited advantages in ACC deaminase activity and potassium solubilization; and P. allii X32 demonstrated high organic phosphorus solubilization capability (3.98 mg/L). Finally, genomic data revealed that P. allii X32 possesses a rich repertoire of PGP-related genes and metabolic pathways, providing a basis for establishing molecular mechanistic hypotheses for these traits. In summary, Pseudomonas strains from different species, which exhibit complementary probiotic functions without antagonism in the A. sinensis microecosystem, provide valuable microbial resources for the ecological cultivation of A. sinensis. Full article

The recognition of targets in ship remote sensing images is crucial for ship collision avoidance, military reconnaissance, and emergency rescue. However, climatic factors such as clouds and fog can obscure and blur remote sensing image targets, leading to missed and false detections in target detection. Therefore, it is necessary to study ship remote sensing target detection that considers the impact of cloud and fog occlusion. Due to the large scale and vast amount of information in remote sensing images, in order to achieve high-precision target detection based on limited resource platforms, a comparison of the detection accuracy and parameter quantity of the YOLO series algorithms was first conducted. Based on the analysis results, the YOLOv8s network model with the least number of parameters while ensuring detection accuracy was selected for lightweight network model improvement. The FasterNet was utilized to replace the backbone feature extraction network of YOLOv8s, and the detection accuracy and lightweight level of the resulting FN-YOLOv8s network model were both improved. Furthermore, structural improvements were made to the AOD-Net dehazing network. By introducing a smoothness loss function, the halo artifacts often generated during the image dehazing process were addressed. Meanwhile, by integrating the atmospheric light value and transmittance, the accumulation error was effectively reduced, significantly enhancing the dehazing effect of remote sensing images. Full article

This study evaluated the surface functionalization of a non-equiatomic TiZrNbTaMo high-entropy alloy (HEA) by micro-arc oxidation (MAO) in Cu-rich electrolytes to tailor its performance for biomedical implants. The Cu content was varied, and the resulting coatings were investigated for their morphology, phase constitution, chemical structure, wettability, and cytocompatibility. X-ray diffraction (XRD) measurements of the substrate indicated a body-centered cubic (BCC) matrix with minor HCP features, while the MAO-treated samples depicted amorphous halo with sparse reflections assignable to CaCO₃, CaO, and CaPO₄. Chemical spectroscopic analyses identified the presence of stable oxides (TiO₂, ZrO₂, Nb₂O₅, Ta₂O₅, MoO₃) and the successful incorporation of bioactive elements (Ca, P, Mg) together with traces of Cu, mainly as Cu₂O. MAO treatment increased surface roughness and rendered a hydrophilic behavior, which are features typically favorable to osseointegration process. In vitro cytotoxic assays with MC3T3-E1 cells (24 h) showed that Cu addition did not induce harmful effects, maintaining or improving cell viability and adhesion compared to the controls. Collectively, MAO in Cu-rich electrolyte yielded porous, bioactive, and Cu-incorporated oxide coatings on TiZrNbTaMo HEA, preserving cytocompatibility and supporting their potential for biomedical applications like orthopedic implants and bone-fixation devices. Full article

Presbyopia-correcting IOLs have revolutionized cataract surgery by enabling functional vision across multiple focal distances, thereby reducing dependence on spectacles. These lenses—ranging from multifocal to extended depth-of-focus (EDOF) and hybrid designs—incorporate advanced optical technologies to address the limitations of traditional monofocal IOLs. Despite their clinical promise, patient satisfaction remains variable, with a substantial subset experiencing postoperative visual discomfort. This review provides a comprehensive overview of presbyopia-correcting IOL technologies, detailing their optical principles, design evolution, and clinical performance. It further analyzes the multifactorial causes of postoperative dissatisfaction, which include optical phenomena such as glare, halos, and reduced contrast sensitivity; ocular comorbidities like dry eye disease, corneal irregular astigmatism, glaucoma, and macular pathology; and surgical variables including IOL centration, pupil size, and biometry accuracy. Additionally, non-physiological factors—such as patient expectations, lifestyle demands, and psychological disposition—play a critical role in perceived outcomes. To address these challenges, the review explores evidence-based strategies for improving satisfaction. These include rigorous preoperative screening for ocular surface disease and aberrations, personalized lens selection based on anatomical and functional criteria, and thorough patient counseling to align expectations with achievable results. Emerging IOL designs that blend multifocal and EDOF features offer promising avenues for minimizing visual disturbances while preserving range of vision. By integrating optical innovation with individualized clinical care, ophthalmologists can enhance postoperative outcomes and optimize real-world satisfaction with presbyopia-correcting IOLs. Full article

This study assesses two Brazilian Type F fly ash samples (FA-A and FA-B), collected from the same thermoelectric complex in different years, to investigate their influence on the production of alkali-activated materials (AAMs). FA-A exhibited a slightly higher SiO₂/Al₂O₃ ratio (3.52 vs. 3.34) and a finer average particle size (D₅₀ = 19.7 μm vs. 30.8 μm) than FA-B. X-ray diffraction revealed that FA-A presented a broad amorphous halo between 15° and 35° (2θ), indicative of phases with low atomic ordering, which are more susceptible to dissolution and capable of supplying Si- and Al-rich species for the formation of alkali activation products. These differences directly affected reactivity and mechanical performance. After 1 day of curing, FA-A-based matrices achieved 88.5 MPa in compressive strength—approximately 100% higher than FA-B (44.2 MPa). However, FA-A suffered a 19.6% strength reduction after 28 days of curing, whereas FA-B showed only a 3.8% decrease over the same period, reflecting better long-term stability. FTIR confirmed Na₂CO₃ formation in FA-A, associated with excess sodium (Na/Al = 2.07 after 28 days), while SEM revealed unreacted spheres persisting in FA-B, consistent with its lower dissolution rate. Water absorption was also significantly different, with FA-B matrices reaching values up to 52% lower than FA-A after 7 days of curing. These results demonstrate that even slight variations in chemical composition and atomic ordering, even for ashes from the same plant, strongly influence the reactivity, microstructure, and mechanical performance of alkali-activated binders. Full article

This manuscript describes the development of a nano-sized synthetic smectic clay hydrogel (LAP) that enables controlled oxygen delivery, making it a promising candidate for treating skin wound infections and promoting healing. LAP is an ingredient in various dermatological products, including powders, creams and emulsions. We investigated the antibacterial effect of the LAP hydrogel by incorporating calcium peroxide (CPO), an oxygen-releasing agent, and measuring the size of the inhibitory halo. We found that CPO hydrogels in LAP showed a significant increase in oxygen release during the first five hours, especially at low CPO concentrations. For example, the hydrogel with 5% CPO showed a controlled release profile with a final percentage oxygen release of 2.47 ± 0.01% after 5 h. In contrast, the hydrogels with 10% and 20% CPO achieved lower final oxygen release values, 0.67 ± 0.01% and 0.75 ± 0.01%, respectively, suggesting that the encapsulation efficiency of LAP is higher at higher concentrations. LAP also proved to be an effective oxygen barrier and showed inherent antimicrobial activity. The research confirmed the antibacterial properties of the hydrogel, with inhibition sites observed against both E. coli and S. aureus. These results emphasize the potential of this hydrogel to serve as an effective tool for wound treatment by providing sustained oxygenation and fighting microbial infections. Full article

Fused filament fabrication (FFF, often called FDM) is widely used in polymer additive manufacturing; however, it suffers from mechanical anisotropy and weak bonding in the Z direction. This work examines how the infill pattern influences the tensile response of PLA parts at fixed printing conditions. Dog-bone specimens (PLA, four patterns: grid, honeycomb, rectilinear, adaptive cubic) were printed and tested in tension (n = 3 per pattern). Grid yielded the highest ultimate tensile strength, whereas honeycomb produced the largest Young’s modulus; rectilinear was intermediate and adaptive cubic was trailed in both metrics. X-ray diffraction of printed PLA showed a broad halo at 16–20° (2θ) with weak α-form reflections, consistent with largely amorphous microstructure after FFF. Together, the results indicate that, at constant material and nominal infill, pattern selection alone can shift the strength–stiffness balance, with grid favoring strength and honeycomb favoring stiffness. Full article

This study aimed to synthesize silver nanoparticles using alcoholic extract of spearmint (Mentha spicata) leaves as a reducing agent and to evaluate their antimicrobial properties. Extract concentrations of 2–5% were used in media with varying pHs. Techniques such as UV-vis spectroscopy, FTIR, and DLS were used to characterize the nanoparticles. The formation of silver nanoparticles was verified by the appearance of a plasmon resonance peak at 418 nm with 2% extract and pH 9. DLS analysis showed a size of 16.1 nm for the 2% extract, which decreased to 10.8 nm with increasing concentration. These results demonstrated that alkaline pH and low extract concentrations favor the formation of monodisperse silver nanoparticles, while higher concentrations induce polydispersity. Silver nanoparticles exhibited antimicrobial activity against E. coli, S. aureus (complete inhibition) and C. albicans (inhibition halo), highlighting their potential in biomedical applications. Full article

Background/Objectives: This study aimed to evaluate the effect of preoperative halo-gravity traction (HGT) on surgical outcomes in adolescents with severe idiopathic scoliosis (AIS), comparing posterior spinal fusion (PSF) performed with versus without traction in terms of curve correction, complication rates, and overall surgical efficacy. Methods: A retrospective cohort study was conducted on 46 adolescents (mean age 14.6 ± 1.9 years) with severe AIS (Cobb > 65°) treated at a single tertiary center between 2011 and 2024. Sixteen patients underwent primary PSF, and 30 received preoperative HGT followed by PSF. Radiographic parameters—including Cobb angle and Risser grade—were analyzed pre- and postoperatively. Statistical tests (t-test, Mann–Whitney U, and multivariable linear regression) assessed differences in correction and predictors of outcome, with p < 0.05 considered significant. Results: Baseline characteristics were comparable between groups (mean preoperative Cobb: 83.6° ± 11.2° vs. 83.1° ± 15.6°, p = 0.91). The traction cohort achieved significantly smaller postoperative Cobb angles (30.9° ± 7.8° vs. 42.7° ± 18.9°, p = 0.027), greater absolute correction (52.7° ± 7.4° vs. 40.4° ± 10.5°, p < 0.001), and higher percentage correction (63.3% ± 6.7% vs. 50.0% ± 14.0%, p = 0.002). Regression analysis confirmed HGT as an independent predictor of improved correction (+14.6%, 95% CI +6.9–22.3%, p = 0.00047). No neurological or major complications occurred, and most correction was achieved within the first three weeks of traction. Conclusions: Preoperative halo-gravity traction significantly enhances deformity correction and surgical safety in severe AIS without added morbidity. Most benefit occurs within 21 days, supporting shorter, standardized traction protocols. HGT remains a valuable adjunct for optimizing outcomes in rigid scoliosis prior to posterior spinal fusion. Full article

Background: Neurofibromatosis 1 is an autosomal dominant disorder accompanied by extensive early-onset spinal manifestations, with or without dystrophic scoliotic features. While non-dystrophic subtypes can often be treated similarly to idiopathic scoliosis, dystrophic scoliosis typically requires more aggressive intervention, often involving instrumentation in severely compromised pedicles or vertebrae. Purpose: This review aims to present recent advances in the surgical treatment of Neurofibromatosis 1-associated scoliosis, including surgical techniques and emerging guidance methods. Methods: An electronic literature search was conducted in Web of Science and PubMed to identify surgical techniques for scoliosis in patients with Neurofibromatosis 1. Results: Forty-one studies on the operative treatment of dystrophic scoliosis or both subtypes were retrieved. Although aggressive treatment with combined anterior and posterior fusion are widely used, posterior-only methods, which avoid plexiform tumours, present encouraging results. Recent studies highlight the effectiveness of growing rod systems in early-onset cases, enabling delayed fusion while preserving T1-S1 growth. Promising results from sectional or segmented correction techniques demonstrate better sagittal balance and Cobb angle correction, respectively. Preoperative use of halo-gravity traction, which has been extensively studied, is associated with reduced neurological impairment and encourages better correction results, avoiding autofusion. Various studies have also reported more precise pedicle screw placement with guidance of O-arm and triggered electromyography (t-EMG). Conclusions: The correction of spinal scoliotic deformities presents a significant challenge. However, recent advances in surgical techniques and intraoperative guidance offer promising strategies for more effective management. Full article

Successful implantation requires a finely regulated endometrial microenvironment during the window of implantation. Chronic endometritis, defined by plasma cell infiltration, and stromal senescence, indicated by p16 expression, represent separate but potentially interacting mechanisms associated with impaired endometrial receptivity. The relationship between these processes remains poorly understood. We aim to examine whether stromal senescence is associated with plasma cell density and clustering in the human endometrium during the implantation window. Forty mid-luteal (LH+7) endometrial biopsies were retrospectively analyzed and stratified into low-senescence (<0.5% stromal p16⁺ cells, n = 20) and high-senescence (>3.5%, n = 20) groups. Plasma cells were identified by immunohistochemistry for MUM1 and CD138 and quantified using HALO^® software (version 3.4). Group comparisons were performed using Student’s t-test and chi-squared analysis. CD138⁺ plasma cells were significantly more abundant in high-senescence endometria than in low-senescence controls (0.065 ± 0.10 vs. 0.014 ± 0.027 cells/mm², p = 0.02). Only MUM1⁺ cells formed stromal clusters, which were more frequent in high-senescence samples (67% vs. 31%, p = 0.05). High endometrial stromal senescence during the implantation window is associated with increased plasma cell infiltration and clustering. This interplay may contribute to chronic endometritis and impaired receptivity, providing new insights into potential diagnostic and therapeutic strategies for reproductive failure. Full article

Contaminated water detoxification remains difficult due to the presence of persistent halo-organic contaminants, such as perfluorooctanoic acid (PFOA) and chlorophenols, which are chemically stable and resist conventional purification methods. Functionalized membrane-based separation and decontamination have garnered immense attention in recent years. Commercially available microfiltration membrane (PVDF) and polymeric non-woven fiber filters (glass and composite) are functionalized with poly(methacrylic acid) (PMAA) that shows outstanding pH-responsive performance and tunable water permeability under ambient conditions perfect for environmental applications. Polymer loading based on weight gain measurements on PMAA–microglass composite fibers (137%) and microglass fibers (116%) confirmed their extent of functionalization, which was significantly greater than that of PVDF (25%) due to its widely effective pore diameter. Presence of chemically active hydrogel within PVDF matrix was validated by FTIR (hydroxyl/carbonyl) stretch peak, substantial decrease in contact angle (68.8° ± 0.5° to 30.8° ± 1.9°), and decrease in pure water flux from 509 to 148 LMH/bar. Nanoparticles are generated both in solution and within PVDF using simple redox reactions. This strategy is extended to PVDF-PMAA membranes, which are loaded with Fe/Pd nanoparticles for catalytic conversion of 4-chlorophenol and PFOA, forming Fe/Pd-PVDF-PMAA systems. A total of 0.25 mg/L Fe/Pd nanoparticles synthesized in solution displayed alloy-type structures and demonstrated a strong catalytic performance, achieving complete hydrogenation of 4-chlorophenol to phenol and 67% hydrogenation of PFOA to its reduced form at 22–23 °C with ultrapure hydrogen gas supply at pH 5.7. These results underscore the potential of hybrid polymer–nanoparticle systems as a novel remediation strategy, integrating tunable separation with catalytic degradation to overcome the limitations of conventional water treatment methods. Full article

Background: Plant growth promoting bacteria (PGPB) are non-pathogenic bacteria that enhance plant growth through several mechanisms such as nutrient mobilization, phytohormones production, defense against phytopathogens, and alleviation of plant stress. Hence, these bacteria are used as ecologic biofertilizers to diminish the use of agrochemicals. Nevertheless, some PGPR strains can harbor antibiotic resistance determinants and the possibility of spreading them upon releasing these bacteria is an environmental concern. Objectives: The objectives of this work are as follows: (1) evaluating the antibiotic resistance in a collection of PGPB, and (2) prospecting antibiotic resistance genes in the genomes of PGPB in order to predict the risk for antibiotic resistance dissemination. Methods: The resistance towards 12 antibiotics in a collection of 20 PGPB (10 Gram-positive and 10 Gram-negative strains) has been evaluated using disk diffusion in agar, broth microdilution, and agar dilution tests. In addition, the whole genomes of six strains have been sequenced in order to find the correlation between the resistance levels and AMR genes by using bioinformatic tools. Results: The results indicated a wide range of halo diameters, but in general Gram-negatives showed higher resistance compared to Gram-positives. The four most resistant strains and the two more susceptible strains were selected for further analysis and sequencing the whole genomes. The resistant strains were identified as Achromobacter spanius N6, Leclercia adecarboxylata H17, Priestia aryabhattai strain MHA1, and Bacillus cereus N25. The susceptible strains were identified as Pantoea sp. S3 and Priestia megaterium MS4. Mining antibiotic resistance genes in the genomes confirmed the existence of resistance determinants responsible for the phenotypic behavior, indicating the potential of genomics for predicting antibiotic resistance in PGPB. However, there was not an exact correspondence between the presence of the genes and the level of resistance, suggesting the existence of additional regulatory mechanisms. Conclusions: The information obtained by genomics must be complemented experimentally by tests for antibiotic resistance determination. In this regard, it is necessary to develop a global antibiotic resistance database for PGPB, due to the difficulty of interpretation of the antibiotic susceptibility tests after comparing the experimental results with those tabulated for clinical species. Full article

Carbonic anhydrase 3 (CA3) exhibits low enzymatic activity compared to other CA isoforms but contains two surface-exposed cysteine residues that undergo glutathionylation under oxidative stress. Highly expressed in muscle tissue, CA3 has been implicated in cellular protection, particularly through interactions with Bcl2-Associated Athanogene 3 (BAG3), modulating autophagy, while CA3 overexpression decreased hypoxia-induced apoptosis in cardiomyocytes. In this study, we investigated the impact of CA3 overexpression on cellular pathways in HEK293T, MDA-MB-231, and SVCT cells using RNA sequencing and proteomics. Gene Set Enrichment Analysis (GSEA) in HEK293T cells revealed the down-regulation of pathways related to protein synthesis, RNA processing, Roundabout signalling, selenocysteine-metabolism, and suppression of neurodegenerative disease-associated pathways. Human breast epithelial cell lines under normoxia and hypoxia showed down-regulation of similar pathways, although notably, hypoxic conditions also suppressed interferon α/β signalling. Proteomic analysis in HEK293T cells using HaloTag pull-down experiments identified putative novel CA3 binding partners, including heat shock 70 kDa proteins 1 and 8, and ribosomal protein S2 (RPS2). RANBP2 protein was consistently up-regulated after CA3 overexpression, irrespective of the presence of CA3 surface-exposed cysteines and HaloTag orientation. These findings suggest that CA3 modulates key cellular processes beyond its enzymatic role, contributing to stress resilience through pathway-level regulation and protein interactions, potentially impacting autophagy and neurodegenerative disease. Full article

Rhamnolipids (RL) are biosurfactants produced mainly by Pseudomonas aeruginosa strains that have environmental and industrial applications. However, their industrial-scale production still faces the challenge of improving the efficiency and cost-effectiveness of the process. The aim of this work was to optimize the cultivation conditions to increase the RL production by using Response Surface Methodology with key parameters of the process, such as oxygen level, agitation, temperature, nutrients, and residual frying oil as a low-cost carbon source. The optimized parameters were 3.04 g/L of nitrogen, 0.5 vvm of aeration and 180 rpm of agitation, with which 52.2 g/L was obtained in 168 h. The critical micellar concentration (CMC) of this RL was 3.14 g/L, and the Oil Spreading assay confirmed the presence of surface-active compounds in the purified RL that generated an average halo area of 2746.7 ± 72.0 mm², which represents an increase of 2063.41% ± 28.36% compared to the negative control. These advances could contribute to more sustainable, cost-effective RL production, promoting its application in bioremediation processes and other industries. Full article