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Keywords = hairpin motors

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18 pages, 1357 KiB  
Article
Ageing Analysis of Hairpin Windings in Inverter-Fed Motor Under PWM Voltage
by Chuxuan He, Stefan Tenbohlen and Michael Beltle
Energies 2025, 18(6), 1376; https://doi.org/10.3390/en18061376 - 11 Mar 2025
Cited by 1 | Viewed by 750
Abstract
The partial discharge (PD) measurement under pulse width modulation (PWM) voltage is a critical measurement of quality assessment for inverter-fed motors, as outlined in IEC 60034-18-41 and IEC 60034-18-42. One of the key parameters in PD measurement is the repetitive partial discharge inception [...] Read more.
The partial discharge (PD) measurement under pulse width modulation (PWM) voltage is a critical measurement of quality assessment for inverter-fed motors, as outlined in IEC 60034-18-41 and IEC 60034-18-42. One of the key parameters in PD measurement is the repetitive partial discharge inception voltage (RPDIV). This paper examines factors that influence the ageing process of hairpin windings in motors, with ageing tests designed using the Design of Experiment (DoE) method. The study focuses on the effects of electrical and thermal stresses on the ageing process. To achieve this, the failure rate, the RPDIV data, and the lifetime data are selected as the output responses. The findings highlights that RPDIV measurements alone cannot accurately predict the degree of ageing of hairpin windings. Specifically, RPDIV results are influenced not only by the quality of the hairpin windings under PWM voltage but also by other contributing factors. Furthermore, the change in RPDIV during the ageing process showed that the RPDIV measurement cannot predict the ageing degree of the hairpin winding. Experimental data on failure rates and lifetimes reveal that both electrical and thermal stresses significantly influence the ageing process, with a notable interaction between these factors. Among the three output responses, the failure rate provides a more accurate reflection of this interaction. To reliably estimate the lifetime of hairpin windings, more precise parameters are necessary. Further research is required to deepen the understanding of the underlying PD mechanisms under PWM voltage, which could enhance diagnostic and predictive capabilities for hairpin winding performance. Full article
(This article belongs to the Special Issue Reliability and Condition Monitoring of Electric Motors and Drives)
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15 pages, 6267 KiB  
Article
Efficiency Optimization of the Main Operating Points of an EV Traction Motor
by Gi-haeng Lee and Yong-min You
Appl. Sci. 2025, 15(1), 368; https://doi.org/10.3390/app15010368 - 2 Jan 2025
Cited by 1 | Viewed by 1132
Abstract
Motor efficiency presents a trade-off between low-speed and high-speed regions. Additionally, the cross-sectional area of hairpin motors employing rectangular wires is larger than that of round wires, thereby amplifying AC copper losses. As the operating speed increases, the AC copper loss also becomes [...] Read more.
Motor efficiency presents a trade-off between low-speed and high-speed regions. Additionally, the cross-sectional area of hairpin motors employing rectangular wires is larger than that of round wires, thereby amplifying AC copper losses. As the operating speed increases, the AC copper loss also becomes more pronounced; therefore, efficiently determining the optimal design point considering these characteristics is essential. This study optimizes the efficiency of an electric vehicle (EV) simulation is conducted using MATLAB 2024, and the main operating points according to the driving cycle are selected. For the EV simulation to select the main operating points, the driving cycle of the multi-cycle test method, which is used for measuring domestic driving range, is considered to enhance the validity of the operating points. The efficiency optimization of the main operating points was performed considering the AC copper loss, and essential parameters such as the torque ripple and total harmonic distortion of the back-electromotive force were incorporated as constraints. Furthermore, the predictive performances of the 11 metamodels were compared to identify the most suitable metamodel for the output and design variables. Subsequently, the selected metamodel was integrated with four optimization algorithms to optimize the design. Full article
(This article belongs to the Section Electrical, Electronics and Communications Engineering)
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20 pages, 16615 KiB  
Article
Asymmetric Hairpin Winding Design for Losses Reduction with Thermal Analysis for an Electric Vehicle Case Study
by Sara M. Ismaeel, Mohamed N. Ibrahim, Essam M. Rashad and Peter Sergeant
Energies 2024, 17(24), 6494; https://doi.org/10.3390/en17246494 - 23 Dec 2024
Cited by 1 | Viewed by 1185
Abstract
The asymmetric design of hairpin windings is known as a method for reducing AC losses in electric motors, especially at high frequencies. However, the design of the asymmetric winding is very critical to obtaining the best benefit regarding the efficiency and the thermal [...] Read more.
The asymmetric design of hairpin windings is known as a method for reducing AC losses in electric motors, especially at high frequencies. However, the design of the asymmetric winding is very critical to obtaining the best benefit regarding the efficiency and the thermal performance of the motor. Compared to the state-of-the-art in this paper, deep investigations are carried out to obtain the optimum design of the asymmetric hairpin windings while still employing a conventional manufacturing method. An analytical model is developed to speed up the investigation process, and the results of the analytical model are validated with a finite element method (FEM) model. The conclusions from the analytical investigation are considered in the design of an electric vehicle (EV) motor. The performance of the motor is studied for two different driving profiles to validate the rules of the asymmetric windings design and check the degree of dependency of the design of asymmetric windings on the application. It is proved that using asymmetric design reduces motor losses and improves thermal performance. Full article
(This article belongs to the Special Issue Optimization and Control of Electric and Hybrid Vehicles)
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24 pages, 8709 KiB  
Article
Residual Stress Analysis at the Conductor–Insulator Interface During the Curing Process of Hair-Pin Motors
by Mingze Ma, Hongyi Gan, Xiao Shang, Linsen Song, Yiwen Zhang, Jingru Liu, Chunbai Liu, Yanzhong Hao and Xinming Zhang
Polymers 2024, 16(24), 3514; https://doi.org/10.3390/polym16243514 - 17 Dec 2024
Cited by 1 | Viewed by 1212
Abstract
The curing process of hair-pin motor stator insulation is critical, as residual stress increases the risk of partial discharge and shortens a motor’s lifespan. However, studies on the stress-induced defects during insulation varnish curing remain limited. This research integrates three-dimensional numerical simulations and [...] Read more.
The curing process of hair-pin motor stator insulation is critical, as residual stress increases the risk of partial discharge and shortens a motor’s lifespan. However, studies on the stress-induced defects during insulation varnish curing remain limited. This research integrates three-dimensional numerical simulations and experimental analysis to develop a curing model based on unsaturated polyester imide resin, aiming to explore the mechanisms of residual stress formation and optimization strategies. A dual fiber Bragg grating (FBG) sensor system is employed for simultaneous temperature and strain monitoring, while curing kinetics tests confirm the self-catalytic nature of the process and yield the corresponding kinetic equations. The multi-physics simulation model demonstrates strong agreement with the experimental data. The results show that optimizing the curing process reduces the maximum stress from 45.1 MPa to 38.6 MPa, effectively alleviating the stress concentration. These findings highlight the significant influence of the post-curing temperature phase on residual stress. The proposed model offers a reliable tool for stress prediction and process optimization in various insulating materials, providing valuable insights for motor insulation system design. Full article
(This article belongs to the Special Issue Application and Characterization of Polymer Composites)
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17 pages, 5327 KiB  
Article
Investigation on the Torsional–Flexural Instability Phenomena during the Bending Process of Hairpin Windings: Experimental Tests and FE Model Validation
by Valerio Mangeruga, Saverio Giulio Barbieri, Matteo Giacopini, Fabrizio Giuradei, Piermaria Vai and Chris Gerada
Machines 2024, 12(6), 396; https://doi.org/10.3390/machines12060396 - 10 Jun 2024
Viewed by 1500
Abstract
Modern electric motors developed for the automotive industry have an ever higher power density with a relatively compact size. Among the various existing solutions to improve torque and power density, a reduction in the dimensions of the end-windings has been explored, aiming to [...] Read more.
Modern electric motors developed for the automotive industry have an ever higher power density with a relatively compact size. Among the various existing solutions to improve torque and power density, a reduction in the dimensions of the end-windings has been explored, aiming to decrease volume, weight, and losses. However, more compact end-windings often lead to complex shapes of the conductors, especially when preformed hairpin windings are considered. The rectangular cross-section of hairpin conductors makes them prone to deviating out of the bending plane during the forming process. This phenomenon, known as torsional–flexural instability, is influenced by the specific aspect ratio of the cross-section dimensions and the bending direction. This study focuses on understanding this instability phenomenon, aiming to identify a potential threshold of the cross-section aspect ratio. The instability makes it difficult to predict the final geometry, potentially compromising the compliance with the geometric tolerances. A finite element model is developed to analyse a single planar bend in a hairpin conductor. Various cross-section dimensions with different aspect ratios are simulated identifying those that experience instability. Moreover, an experimental campaign is conducted to confirm the occurrence of instability by testing the same single planar bending. The experimental data obtained are used to validate the finite element model for the tested dimensions. The aim is to provide designers with a useful tool to select hairpin geometries that are more suitable for the folding process, contributing to successful assembly and improving the overall design process of preformed hairpin conductors. Full article
(This article belongs to the Special Issue Advances in Design and Manufacturing in Die Casting and Metal Forming)
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21 pages, 2574 KiB  
Article
AAV-Mediated CAG-Targeting Selectively Reduces Polyglutamine-Expanded Protein and Attenuates Disease Phenotypes in a Spinocerebellar Ataxia Mouse Model
by Anna Niewiadomska-Cimicka, Lorraine Fievet, Magdalena Surdyka, Ewelina Jesion, Céline Keime, Elisabeth Singer, Aurélie Eisenmann, Zaneta Kalinowska-Poska, Hoa Huu Phuc Nguyen, Agnieszka Fiszer, Maciej Figiel and Yvon Trottier
Int. J. Mol. Sci. 2024, 25(8), 4354; https://doi.org/10.3390/ijms25084354 - 15 Apr 2024
Cited by 8 | Viewed by 2616
Abstract
Polyglutamine (polyQ)-encoding CAG repeat expansions represent a common disease-causing mutation responsible for several dominant spinocerebellar ataxias (SCAs). PolyQ-expanded SCA proteins are toxic for cerebellar neurons, with Purkinje cells (PCs) being the most vulnerable. RNA interference (RNAi) reagents targeting transcripts with expanded CAG reduce [...] Read more.
Polyglutamine (polyQ)-encoding CAG repeat expansions represent a common disease-causing mutation responsible for several dominant spinocerebellar ataxias (SCAs). PolyQ-expanded SCA proteins are toxic for cerebellar neurons, with Purkinje cells (PCs) being the most vulnerable. RNA interference (RNAi) reagents targeting transcripts with expanded CAG reduce the level of various mutant SCA proteins in an allele-selective manner in vitro and represent promising universal tools for treating multiple CAG/polyQ SCAs. However, it remains unclear whether the therapeutic targeting of CAG expansion can be achieved in vivo and if it can ameliorate cerebellar functions. Here, using a mouse model of SCA7 expressing a mutant Atxn7 allele with 140 CAGs, we examined the efficacy of short hairpin RNAs (shRNAs) targeting CAG repeats expressed from PHP.eB adeno-associated virus vectors (AAVs), which were introduced into the brain via intravascular injection. We demonstrated that shRNAs carrying various mismatches with the CAG target sequence reduced the level of polyQ-expanded ATXN7 in the cerebellum, albeit with varying degrees of allele selectivity and safety profile. An shRNA named A4 potently reduced the level of polyQ-expanded ATXN7, with no effect on normal ATXN7 levels and no adverse side effects. Furthermore, A4 shRNA treatment improved a range of motor and behavioral parameters 23 weeks after AAV injection and attenuated the disease burden of PCs by preventing the downregulation of several PC-type-specific genes. Our results show the feasibility of the selective targeting of CAG expansion in the cerebellum using a blood–brain barrier-permeable vector to attenuate the disease phenotype in an SCA mouse model. Our study represents a significant advancement in developing CAG-targeting strategies as a potential therapy for SCA7 and possibly other CAG/polyQ SCAs. Full article
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14 pages, 2203 KiB  
Article
Calsarcin-2 May Play a Compensatory Role in the Development of Obese Sarcopenia
by Yu-Cheng Liang, Kai-Pi Cheng, Hsin-Yu Kuo, Chung-Teng Wang, Hsuan-Wen Chou, Kuan-Lin Huang, Hung-Tsung Wu and Horng-Yih Ou
Biomedicines 2023, 11(10), 2708; https://doi.org/10.3390/biomedicines11102708 - 5 Oct 2023
Cited by 2 | Viewed by 1719
Abstract
Although obese sarcopenia is a major public health problem with increasing prevalence worldwide, the factors that contribute to the development of obese sarcopenia are still obscure. In order to clarify this issue, a high-fat-diet-induced obese sarcopenia mouse model was utilized. After being fed [...] Read more.
Although obese sarcopenia is a major public health problem with increasing prevalence worldwide, the factors that contribute to the development of obese sarcopenia are still obscure. In order to clarify this issue, a high-fat-diet-induced obese sarcopenia mouse model was utilized. After being fed with a high-fat diet for 24 weeks, decreased motor functions and muscle mass ratios were found in the C57BL/6 mice. In addition, the expression of calsarcin-2 was significantly increased in their skeletal muscle, which was determined by a microarray analysis. In order to clarify the role of calsarcin-2 in muscle, lentiviral vectors containing the calsarcin-2 gene or short hairpin RNA targeted to calsarcin-2 were used to manipulate calsarcin-2 expressions in L6 myoblasts. We found that an overexpression of calsarcin-2 facilitated L6 myoblast differentiation, whereas a calsarcin-2 knockdown delayed myoblast differentiation, as determined by the expression of myogenin. However, the calsarcin-2 knockdown showed no significant effects on myoblast proliferation. In addition, to clarify the relationship between serum calsarcin-2 and sarcopenia, the bilateral gastrocnemius muscle mass per body weight in mice and appendicular skeletal muscle mass index in humans were measured. Although calsarcin-2 facilitated myoblast differentiation, the serum calsarcin-2 concentration was negatively related to skeletal muscle mass index in mice and human subjects. Taken together, calsarcin-2 might facilitate myoblast differentiation and appear to play a compensatory role in sarcopenia. Full article
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14 pages, 5831 KiB  
Article
Hairpin Windings for Electric Vehicle Motors: Modeling and Investigation of AC Loss-Mitigating Approaches
by Payam Shams Ghahfarokhi, Andrejs Podgornovs, Antonio J. Marques Cardoso, Ants Kallaste, Anouar Belahcen and Toomas Vaimann
Machines 2022, 10(11), 1029; https://doi.org/10.3390/machines10111029 - 4 Nov 2022
Cited by 14 | Viewed by 6276
Abstract
The hairpin winding configuration has been attracting attention as a solution to increase the power density of electric vehicle motors by enhancing the slot-filling factor. However, this winding configuration brings high AC losses during high-speed operation and we require new approaches to tackle [...] Read more.
The hairpin winding configuration has been attracting attention as a solution to increase the power density of electric vehicle motors by enhancing the slot-filling factor. However, this winding configuration brings high AC losses during high-speed operation and we require new approaches to tackle this challenge. This paper considers reducing AC losses by proposing two main methods: correct transposition of conductors in parallel paths, and enhancing the number of conductor layers in a slot. First, the proper connection of conductors in parallel paths is considered, and the essential rules for this purpose are described. Next, the paper uses a numerical approach to deal with the effect of incorrect conductor transposition in winding paths on generating additional AC losses due to circulating currents. Finally, the impact of the number of conductor layers in the mitigation of AC losses is also discussed in detail. According to the results, by increasing the number of layers, ohmic losses in the layer near the slot opening dramatically decrease. For instance, ohmic losses in the layer near the slot opening of the eight-layer setup were 82% less than the two-layer layout. Full article
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13 pages, 5852 KiB  
Communication
Investigation of the Temperature Effects on Copper Losses in Hairpin Windings
by Mohammad Soltani, Stefano Nuzzo, Davide Barater and Giovanni Franceschini
Machines 2022, 10(8), 715; https://doi.org/10.3390/machines10080715 - 20 Aug 2022
Cited by 5 | Viewed by 3063
Abstract
Today, an extensive electrification is occurring in all industrial sectors, with a special interest seen in the automotive and aerospace industries. The electric motor, surely, is one of the main actors in this context, and an ever-increasing effort is spent with the aim [...] Read more.
Today, an extensive electrification is occurring in all industrial sectors, with a special interest seen in the automotive and aerospace industries. The electric motor, surely, is one of the main actors in this context, and an ever-increasing effort is spent with the aim of improving its efficiency and torque density. Hairpin windings are one of the recent technologies which are implemented onto the stator of the electric motor. Compared to conventional random windings, it inherently features lower DC resistance, higher fill factor, better thermal performance, improved reliability, and an automated manufacturing process. However, its bottleneck is the high ohmic losses at high-frequency operations due to skin and proximity effects (AC losses), resulting in a negative impact on the temperature map of the machine. Nevertheless, while it is well-known that DC losses increase linearly with the operating temperatures, the AC losses trend needs further insight. This paper demonstrates that operating the machine at higher temperatures could be beneficial for overall efficiency, especially at high-frequency operations. This suggests that a paradigm shift is required for the design of electric motors equipped with hair-pin windings, which should therefore focus on a temperature-oriented approach. In addition, the effect of the rotor topology on AC losses, which is often overlooked, is also considered in this paper. The combination of these effects is used to carry out observations and, eventually, to provide design recommendations. Finite element electromagnetic and thermal evaluations are performed to prove the findings of this research. Full article
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19 pages, 8037 KiB  
Article
Impact of Wire Selection on the Performance of an Induction Motor for Automotive Applications
by Lino Di Leonardo, Giuseppe Fabri, Andrea Credo, Marco Tursini and Marco Villani
Energies 2022, 15(11), 3876; https://doi.org/10.3390/en15113876 - 24 May 2022
Cited by 9 | Viewed by 3473
Abstract
This paper investigates the impact of the wire selection on the performance of induction motors for automotive applications. The section of wire and the material are evaluated at a high speed of 200 kW in an induction motor designed for premium vehicle applications. [...] Read more.
This paper investigates the impact of the wire selection on the performance of induction motors for automotive applications. The section of wire and the material are evaluated at a high speed of 200 kW in an induction motor designed for premium vehicle applications. The proposed solutions have the same electromagnetic and thermal constraints, as well as the same final encumbrance. The various wire and winding types differ in terms of slot design, phase resistance, end-winding overhanging portion, skin and proximity effects, and equivalent slot thermal conductivity. Their impacts are analyzed in terms of the operating area motor efficiency and they are tested in an automotive drive cycle, highlighting the advantages and disadvantages of each configuration. Full article
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12 pages, 3471 KiB  
Article
Optimization of a Permanent Magnet Synchronous Motor for e-Mobility Using Metamodels
by Se-eun Kim and Yong-min You
Appl. Sci. 2022, 12(3), 1625; https://doi.org/10.3390/app12031625 - 3 Feb 2022
Cited by 12 | Viewed by 7441
Abstract
Permanent magnet synchronous motors (PMSMs) with rectangular coils in hairpin windings exhibit improved fill factor and reduced end turn of the coils, which in turn improve the efficiency and power density of PMSMs, making them ideal for e-mobility applications. Herein, the shape of [...] Read more.
Permanent magnet synchronous motors (PMSMs) with rectangular coils in hairpin windings exhibit improved fill factor and reduced end turn of the coils, which in turn improve the efficiency and power density of PMSMs, making them ideal for e-mobility applications. Herein, the shape of a PMSM was optimized for torque ripple reduction using metamodels to improve the noise and vibrational performance of the motor. The objective function of the optimal design aimed to minimize the torque ripple, and the average torque and efficiency were set as constraints. The notch width and depth and barrier length were selected as the design variables to satisfy the objective function and constraints. Using the optimal Latin hypercube design technique, 27 experimental points were selected, and a finite element analysis (FEA) was performed for each point. Furthermore, a function approximation was performed using six metamodels, and the best metamodel was selected using the root mean square error test. Moreover, the optimization was performed by combining the best metamodels for each variable with a sequential two-point diagonal quadratic approximation optimization algorithm. The torque ripple was improved by approximately 1.63% compared with the initial model, whereas the constraint values remained constant. Finally, an FEA was performed on the optimal point, and the FEA results matched with those of the optimal method. Full article
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15 pages, 42792 KiB  
Article
Experimental Study on Behavior of Coolants, Particularly the Oil-Cooling Method, in Electric Vehicle Motors Using Hairpin Winding
by Taewook Ha, Nyeon Gu Han, Min Soo Kim, Kyu Heon Rho and Dong Kyu Kim
Energies 2021, 14(4), 956; https://doi.org/10.3390/en14040956 - 11 Feb 2021
Cited by 30 | Viewed by 5115
Abstract
This paper analyzes the characteristics of oil behavior in the oil-cooling of motors with hairpin winding to understand how to maximize cooling performance. The oil cooling is performed by directly spraying oil onto the motor components. The results show that as the temperature [...] Read more.
This paper analyzes the characteristics of oil behavior in the oil-cooling of motors with hairpin winding to understand how to maximize cooling performance. The oil cooling is performed by directly spraying oil onto the motor components. The results show that as the temperature of the oil increases, the viscosity decreases, and the oil film is formed more evenly; however, oil splashing also increases. Similarly, as the flow rate increases, oil splashing also increases, but the amount of oil forming the oil film increases. However, the oil film is not affected by the rotor’s rotation. In contrast, the immersed oil is found to be closely related to the rotor’s rotation. As the rotational speed increases, the immersion oil is mixed with the air, and oil churning occurs. The mixing phenomenon increases as the temperature and flow rate of the oil increases. The higher the oil level, the greater the oil churning. As the oil is mixed with air, the heat transfer coefficient decreases, which adversely affects the thermal management of the motor. As a result, when considering the oil film and the immersion oil, the optimal oil temperature, flow rate, and oil level are at 60 °C, 0.140 kg/s, and 85 mm, respectively. The results of this paper give important information about EV motor cooling and can contribute to the development of high-performance motors. Full article
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15 pages, 5805 KiB  
Article
Study of Injection Method for Maximizing Oil-Cooling Performance of Electric Vehicle Motor with Hairpin Winding
by Taewook Ha and Dong Kyu Kim
Energies 2021, 14(3), 747; https://doi.org/10.3390/en14030747 - 1 Feb 2021
Cited by 39 | Viewed by 6074
Abstract
The oil injection method was studied to maximize the cooling performance of an electric vehicle motor with a hairpin winding. The cooling performance of the motor using the oil cooling method is proportional to the contact area of the oil and the coil. [...] Read more.
The oil injection method was studied to maximize the cooling performance of an electric vehicle motor with a hairpin winding. The cooling performance of the motor using the oil cooling method is proportional to the contact area of the oil and the coil. A numerical analysis was conducted to examine the effect of the spray nozzle type on the oil flow. The dripping nozzle forms the thickest oil film on the coil, making it the most effective for cooling of hairpin-type motors. Subsequently, an experimental study was conducted to optimize the nozzle diameter and number of nozzles. When the inlet diameter and number was 6.35 mm and 6, the oil film formation rate was 53%, yielding the most uniform oil film. Next, an experiment was performed to investigate the effects of the oil temperature and flow rate on the oil flow. The oil film formation rate was the highest (83%) when the oil temperature was 40 °C and the flow rate was 6 LPM. Full article
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25 pages, 10313 KiB  
Article
N-Acetyl-d-Glucosamine Kinase Interacts with NudC and Lis1 in Dynein Motor Complex and Promotes Cell Migration
by Md. Ariful Islam, Ho Jin Choi, Raju Dash, Syeda Ridita Sharif, Diyah Fatimah Oktaviani, Dae-Hyun Seog and Il Soo Moon
Int. J. Mol. Sci. 2021, 22(1), 129; https://doi.org/10.3390/ijms22010129 - 24 Dec 2020
Cited by 17 | Viewed by 4955
Abstract
Recently, we showed that N-acetylglucosamine kinase (NAGK), an enzyme of amino sugar metabolism, interacts with dynein light chain roadblock type 1 (DYNLRB1) and promotes the functions of dynein motor. Here, we report that NAGK interacts with nuclear distribution protein C (NudC) and [...] Read more.
Recently, we showed that N-acetylglucosamine kinase (NAGK), an enzyme of amino sugar metabolism, interacts with dynein light chain roadblock type 1 (DYNLRB1) and promotes the functions of dynein motor. Here, we report that NAGK interacts with nuclear distribution protein C (NudC) and lissencephaly 1 (Lis1) in the dynein complex. Yeast two-hybrid assays, pull-down assays, immunocytochemistry, and proximity ligation assays revealed NAGK–NudC–Lis1–dynein complexes around nuclei, at the leading poles of migrating HEK293T cells, and at the tips of migratory processes of cultured rat neuroblast cells. The exogenous expression of red fluorescent protein (RFP)-tagged NAGK accelerated HEK293T cell migration during in vitro wound-healing assays and of neurons during in vitro neurosphere migration and in utero electroporation assays, whereas NAGK knockdown by short hairpin RNA (shRNA) delayed migration. Finally, a small NAGK peptide derived from the NudC interacting domain in in silico molecular docking analysis retarded the migrations of HEK293T and SH-SY5Y cells. These data indicate a functional interaction between NAGK and dynein–NudC–Lis1 complex at the nuclear envelope is required for the regulation of cell migration. Full article
(This article belongs to the Special Issue Regulation of Gene Expression During Embryonic Development)
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13 pages, 5308 KiB  
Article
Structure of Salmonella Flagellar Hook Reveals Intermolecular Domain Interactions for the Universal Joint Function
by Péter Horváth, Takayuki Kato, Tomoko Miyata and Keiichi Namba
Biomolecules 2019, 9(9), 462; https://doi.org/10.3390/biom9090462 - 9 Sep 2019
Cited by 20 | Viewed by 4265
Abstract
The bacterial flagellum is a motility organelle consisting of a rotary motor and a long helical filament as a propeller. The flagellar hook is a flexible universal joint that transmits motor torque to the filament in its various orientations that change dynamically between [...] Read more.
The bacterial flagellum is a motility organelle consisting of a rotary motor and a long helical filament as a propeller. The flagellar hook is a flexible universal joint that transmits motor torque to the filament in its various orientations that change dynamically between swimming and tumbling of the cell upon switching the motor rotation for chemotaxis. Although the structures of the hook and hook protein FlgE from different bacterial species have been studied, the structure of Salmonella hook, which has been studied most over the years, has not been solved at a high enough resolution to allow building an atomic model of entire FlgE for understanding the mechanisms of self-assembly, stability and the universal joint function. Here we report the structure of Salmonella polyhook at 4.1 Å resolution by electron cryomicroscopy and helical image analysis. The density map clearly revealed folding of the entire FlgE chain forming the three domains D0, D1 and D2 and allowed us to build an atomic model. The model includes domain Dc with a long β-hairpin structure that connects domains D0 and D1 and contributes to the structural stability of the hook while allowing the flexible bending of the hook as a molecular universal joint. Full article
(This article belongs to the Special Issue Perspectives on Bacterial Flagellar Motor)
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