Search Results (442)

The increasing demand for low-alcohol wine products calls for effective vineyard strategies to reduce grape sugar concentration, while climate change is exacerbating sugar accumulation through warmer growing conditions. In this context, severe shoot trimming applied at specific phenological stages may represent a promising approach to induce sustained source limitation. A field experiment was conducted in 2025 on Vitis vinifera L. cv. Ortrugo to evaluate severe shoot trimming performed at the onset of berry softening. Vine growth, yield components, grape composition, and seasonal total soluble solids (TSSs) were monitored. Vine carbon reserves and shoot fruitfulness were assessed to evaluate carry-over effects. Experimental wines were produced to determine alcohol and fermentative aroma. Severe trimming markedly reduced leaf area and vine balance, leading to a sustained reduction in sugar accumulation. At harvest, grape TSSs decreased by 4.1 °Brix (17.6 vs. 21.7 °Brix) and the final wine alcohol concentration was lower by 3.4% (v/v). Yield was unaffected and no substantial negative effects on wine fermentative aroma were observed, while titratable acidity slightly increased. Even if trimming reduced winter starch concentration in roots, no reduction in shoot fruitfulness was observed in the subsequent spring. Severe trimming successfully reduced grape sugar and wine ethanol without compromising yield, aroma, or vine performance, supporting its potential for low-alcohol wine production and reduced-impact dealcoholization. Full article

This research focused on the formulation of a health-oriented, clean-label food product fortified with encapsulated bioactive compounds from Sambucus nigra, Aronia melanocarpa, and Echinacea purpurea. To evaluate the protection of these sensitive compounds during production and storage, a comprehensive characterization was performed. This included basic physicochemical analyses, phenolic profiling, antioxidant activity tests, as well as rheological and textural measurements. Furthermore, sensory analysis, consumer evaluation, and microbiological stability during storage were assessed. Results from Scanning Electron Microscopy (SEM) and Fourier-Transform Infrared Spectroscopy (FTIR) analyses confirmed the structural integrity of the capsules post-processing. Additionally, the application of a starch–psyllium carrier ensured that the textural and rheological properties remained fully comparable to the control sample, preventing undesirable matrix alterations. Specifically, product hardness (1.17–1.23 N) and adhesiveness (8.17–8.94 N·s) were maintained at stable levels, while color alterations were minor and likely noticeable only to trained observers (ΔE* < 3.2). Microbiological evaluation demonstrated that the application of different formulated products effectively inhibited the growth of Gram-positive and Gram-negative bacterial strains, with inhibition rates increasing from 3.4 to 39.7%. Collectively, the experimental data demonstrate that encapsulation is a highly effective strategy for fortifying fruit-based systems with sensitive extracts, successfully maximizing bioactivity retention while maintaining high product quality and sensory appeal. Full article

Fruit and vegetable processing by-products, such as peels and pomace, are rich in antioxidant polyphenols and represent promising sources of functional ingredients, but how their galloyl-based polyphenols interact with starch remains insufficiently understood. In this study, corilagin with three non-free galloyl moieties and 1,2,3,4,6-O-pentagalloyl glucose with five free galloyl moieties were used as model polyphenols to clarify how galloyl moiety number and accessibility modulate their complexation with high-amylose maize starch (HAMS). Size-exclusion chromatography showed that both polyphenols preferentially complexed with amylose, while FTIR confirmed that complex formation occurred mainly through non-covalent interactions. The two polyphenols induced distinct changes in HAMS structure. Corilagin disrupted short-range order and produced no detectable crystalline structure, whereas 1,2,3,4,6-O-pentagalloyl glucose enhanced molecular order and induced V-type crystallization. Isothermal titration calorimetry revealed more binding sites but weaker affinity for corilagin, with thermodynamic signatures indicating hydrogen bonding and van der Waals interactions. By contrast, 1,2,3,4,6-O-pentagalloyl glucose showed stronger affinity and hydrophobic interaction-dominated binding. Molecular dynamics simulations further confirmed that 1,2,3,4,6-O-pentagalloyl glucose formed a more stable association with the amylose helix than corilagin. These results indicate that galloyl moiety characteristics markedly influence starch–polyphenol interaction mechanisms, providing guidance for the utilization of polyphenol-rich agro-processing by-products in functional starch-based foods. Full article

Sustainable starch-based bioplastics have emerged as promising alternatives to conventional plastics for fresh produce packaging, yet their efficacy in preserving highly perishable fruits remains underexplored. Strawberries cv. San Andreas, prone to rapid postharvest deterioration, require packaging that balances moisture retention and gas exchange to maintain quality. This study developed lucuma seed starch-based bioplastics incorporated with free (EO) or microencapsulated (EOM) lemon verbena essential oil and evaluated their performance during 16 days of refrigerated storage (4 °C) compared to non-active bioplastic (Control) and commercial low-density polyethylene (LDPE). Microencapsulation enhanced the stability and controlled release of bioactive compounds. The EOM treatment reduced weight loss to 12.81% (vs. 18.25% in Control and 6.29% in LDPE), while preserving firmness at 3.87 N (vs. 2.19 N in LDPE). Strawberries packaged in both EO and EOM exhibited complete suppression of visible decay (0% incidence) throughout storage, in stark contrast to LDPE (57.34% incidence). The EOM system also maintained higher levels of total phenolics (205.51 mg GAE/100 g FW), antioxidant capacity (289.05 µmol TE/100 g FW), and anthocyanins compared to LDPE and Control treatments. These findings demonstrate that lucuma seed starch bioplastics containing microencapsulated lemon verbena essential oil represent a sustainable and functional packaging strategy to extend shelf life and preserve the quality of highly perishable strawberries during refrigerated storage. Full article

This study aimed to characterize corn (CS), wolf fruit (WF), and butterfly lily (BL) starches; to develop bioactive coatings from pure starches and their binary and ternary blends; and to evaluate the synergistic effects of these formulations on the physiological quality of common bean seeds. Films were prepared by thermocompression (80 °C, 6 min, 3 t) of film-forming solutions obtained via microwave processing and formulated using a simplex-centroid mixture design. The starches were characterized in terms of amylose content, Scanning Electron Microscopy, Fourier Transform Infrared Spectroscopy, Differential Scanning Calorimetry, Rapid Visco Analyser, while the films were evaluated for thickness, water solubility, and water vapor permeability. The film-forming solutions were applied as coatings, and seed physiological quality was assessed through germination, first count, seedling length, and dry mass. BL exhibited higher gelatinization temperatures and produced films with adequate thickness and moderate permeability, indicating greater structural stability. The CS:BL blend produced films with balanced hydration, promoting rapid and uniform water uptake. Coatings based on BL and CS:BL showed the highest germination percentages, whereas CS:WF resulted in lower physiological performance. These results demonstrate that film properties directly influence seed vigor and germination. BL, alone or blended with CS, represents a promising starch-based material for seed coating, promoting high physiological quality and environmentally friendly characteristics. Full article

The environmental impact of food waste and agro-industrial by-products has promoted the development of circular economy strategies for food applications. In this study, edible films were developed from biopolymers extracted from avocado peel and seeds (hemicellulose, pectin, lignin, and starch), incorporating ethyl lauroyl arginate (LAE^®) as an antifungal agent. The activity of LAE^® was evaluated against Colletotrichum gloeosporioides on inoculated avocados stored at 12 °C and 22 °C. Fruit shelf life was assessed through physiological, physicochemical and sensory parameters during cold storage and subsequent shelf life. Films containing 10% LAE^® exhibited strong antifungal activity, and their efficacy was higher at 12 °C than at 22 °C. Coated fruits exhibited a ripening delay of up to 2 days compared to controls. These findings highlight the potential use of avocado by-product-based LAE^® coatings as a sustainable strategy for preserve postharvest avocado quality. Full article

The genus Cucurbita (pumpkin), encompassing C. maxima and C. moschata, is agriculturally and nutritionally significant globally. Herein, we re-sequenced 146 germplasm accessions (51 C. moschata and 95 C. maxima) to characterize genomic variations and identify loci associated with critical traits. Population structure analysis revealed four distinct subgroups: G1 (C. moschata), and G2–G4 (C. maxima), the latter classified by rind color (green, gray, and red, respectively). A genome-wide association study (GWAS) analysis identified 26 loci associated with eight fruit quality traits (e.g., rind color, pulp thickness, starch content) and leaf traits. Selective sweep analyses revealed 18 overlapping signals between the GWAS and selective regions, highlighting convergent evolution in starch content, pulp thickness, and water content driven by artificial and natural selection. We further validated these key fruit quality candidate genes, confirming that starch, cellulose, and pulp trait-related genes exhibited genotype-specific expression consistent with the quality divergence between CMO-X and CMO-E. Notably, C. moschata exhibited higher β-carotene and water content, while C. maxima showed higher starch content, reflecting divergent selection pressures. For leaf traits, 13 loci associated with leaf length were found, including LL9.2/LW9.1 with Cmax09G001045, which regulates leaf size. A novel haplotype in Cmax09G001045 explained the small-leaf phenotype of ‘Cuili 5’. This study provides a comprehensive genomic variation map of C. maxima and C. moschata, clarifies the genetic bases of key agronomic traits, and sheds light on their domestication and selection history, offering valuable resources for molecular breeding and crop improvement. Full article

Daddow is a macadamia cultivar that yields poorly because flowers and fruitlets frequently abort early in development. The objective was to determine whether resource availability limits fruitlet retention in this cultivar. Racemes of Daddow and three other cultivars, 849, A38, and A203, were sampled weekly during flowering and fruit set, and trends in nutrients and non-structural carbohydrates were assessed. Starch concentrations in the flowers and rachis were lower in Daddow than in the other cultivars before fruit set. Rachis concentration of glucose, an important signalling molecule, was also lower in Daddow at flowering. Most flower and fruitlet nutrient concentrations in Daddow were comparable to those of the other cultivars. However, potassium concentrations were lower in both the rachis and leaves of Daddow, while boron concentration, important for pollen tube growth, was higher. These results suggest altered signalling mechanisms, inadequate carbohydrate supply and deficiencies in specific nutrients may have been contributing factors to the high flower and fruitlet abortion rates of Daddow. Full article

To enrich the breeding resources of Xanthoceras sorbifolium and provide support for its industrialization, we conducted multi-trait comparisons and comprehensive evaluations of 48 elite X. sorbifolium clones with high oil contents and high comprehensive utilization values, based on germplasms preserved at Qiuxian Forest Farm, Handan, Hebei Province, China. The coefficients of variation for quantitative traits were 7.04–74.16%, with variability being greatest in shell and fruit weights. Cluster analysis categorized the 48 germplasms into four distinct groups, with marked inter-group trait differences. Soluble protein, soluble sugar, and starch contents varied significantly among accessions. The average kernel oil content was 60.30%, differences in oil content were validated at the cellular level through microscopic oil body observations. Correlation analysis demonstrated that comprehensive oil content was strongly significantly positively correlated with fruit- and seed-related traits, whereas oil content was strongly significantly negatively correlated with fruit shell thickness and seed coat thickness. Principal component analysis extracted five principal components with a cumulative contribution rate of 72.747%, encapsulating four core information dimensions: fruit and seed size, oil content, protein quality, and energy substances. Membership function analysis, selected germplasms G10, G2, G4, G7, G20, G24, G17, G1, G34, and G5 as optimal accessions. These clones are suitable core breeding materials due to their outstanding performance in terms of nutrient content, geometric characteristics, and fruit and seed weights. Notably, G24, G5, and G17 demonstrated significant advantages in oil quality with high linoleic acid (C18:2), oleic acid (C18:1), and nervonic acid (C24:1) contents, respectively, indicating the excellent potential of X. sorbifolium development for oil production. Full article

The transition of blueberry fruits from the ripening stage to the post-harvest senescence stage is rapid. However, the internal physiological, biochemical, and molecular mechanisms underlying this process have not been elucidated. This study analyzed changes during blueberry fruit development and post-harvest storage senescence to examine processes associated with quality loss. Post-harvest senescence was associated with a marked metabolic transition, which coincided with a transient ethylene peak at maturity and the accumulation of sugars and anthocyanins. This ripening phase was followed by increased oxidative stress, reflected in higher membrane damage, elevated malondialdehyde (MDA) levels, and shifts in conductivity and antioxidant activities, including reduced superoxide dismutase (SOD) levels and increased catalase (CAT), peroxidase (POD), and polyphenol oxidase (PPO) activities. The oxidative conditions were associated with higher rotting rates and coincided with fruit softening and increased polygalacturonase (PG) and β-galactosidase (β-Gal) activities. Correlation analysis identified a “ripening stress-activation cluster” (ethylene, sugars, anthocyanin, CAT/POD/PPO, MDA, PG/β-Gal, and RR) and a “textural integrity cluster” (hardness, chewiness, titratable acidity (TA), and ascorbic acid); these clusters represent correlation-based groupings of variables rather than experimentally validated functional modules. Transcriptomic profiling further showed extensive gene expression changes during storage. Functional enrichment analysis supported a shift from developmental metabolism toward senescence-associated pathways, including starch and sucrose metabolism, phenylpropanoid biosynthesis, flavonoid biosynthesis, mitogen activated protein kinase (MAPK) signaling, and cell wall modification. Overall, the results support a model in which ethylene signaling, oxidative stress, and cell wall disassembly are associated with the transition from ripening to senescence, offering insights for improving blueberry post-harvest quality and extending shelf life. Full article

Background/Objectives: Kazakhstan currently lacks representative data on the actual consumption of calories, macronutrients, and micronutrients among students, despite the rising interest in the subject of children’s nutrition. The objective of the study was to assess the actual nutritional status of urban schoolchildren and identify differences depending on demographic and socioeconomic factors. Methods: A 24 h dietary survey was used. The final analytical dataset included 865 children aged 7 to 17 years, representing only the two specified ethnocultural groups. We divided these participants into three groups according to standard age classification: 7 to 10 years, 11 to 14 years, and 15 to 17 years. We calculated the nutrient profile using national and international food composition databases. Results: We found an age-related trend toward increased consumption of fluids, proteins, fats, and several micronutrients. Adolescents (15–17 years) had a higher intake of simple sugars and a lower intake of starch compared to other age groups. Age, gender, ethnicity, and socioeconomic factors (family income and food expenditure) were statistically significantly associated with BMI and diet composition, with caloric intake and essential nutrient intake increasing with age. Conclusions: The results emphasize the need for comprehensive interventions that go beyond school meals, such as controlling the food environment, lowering the availability of ultra-processed foods, boosting the availability of dairy products, fruits, and vegetables, and creating mechanisms for tracking and assessing the efficacy of Kazakhstan’s revised school meal standards. Full article

Ormosia macrocalyx grows in tropical forests and is endangered in Mexico. The species has ecological and economic importance. To evaluate the relationship between fruit length and seed number, Pearson correlation and principal component analysis were used. A linear mixed-effects model was also applied. Pearson correlation, principal components analysis (PCA) and a Linear Mixed-Effects Model (LMM) were performed on an exploratory basis. In addition, seed coat and cotyledon anatomy were examined, and histochemical tests for secondary metabolites were carried out. Two high correlations and two components were obtained from the PCA, and the LMM showed that fruit length influenced the number of seeds per fruit. In the seed coats, differentiated layers of macrosclereids and osteosclereids were identified, where the hilar region presented macrosclereids and a pyriform bar of tracheids, the reserved cotyledons showed double-walled cells and simple plasmodesmata, the histochemical analyses demonstrated the presence of cellulose, condensed tannins, lipids, alkaloids, and proteins, and no starch was present. This study provides the first description of seed coat and cotyledon anatomy in O. macrocalyx, as well as the first report of secondary metabolites in storage cotyledons. These results could be useful for further studies of this species. Full article

Tomato (Solanum lycopersicum) is a globally important vegetable crop, with fruit quality being a major focus of research. Starch serves as the primary carbohydrate reserve during early fruit development and functions as a key carbon precursor for flavor compound biosynthesis in later stages. To elucidate the role of starch accumulation in determining ripe fruit quality, we analyzed the relationship between starch content in mature green fruits and flavor-related traits across eight tomato cultivars. The results demonstrated that starch content at the mature green stage showed a significantly positive correlation with total soluble solids (TSS) content (r = 0.922) and a significantly positive correlation with total acidity content (r = 0.783) in red-ripe fruits. Furthermore, the expression levels of starch synthesis gene AGPS1 and degradation gene PWD at the mature green stage were both significantly positively correlated with the final fruit TSS levels. These findings highlight the important role of starch accumulation during the mature green stage in shaping final fruit quality, providing a theoretical basis for breeding high-quality tomato varieties. Full article

This study addresses the imperative need to extend the shelf life of the cape gooseberry (Physalis peruviana L.), a highly perishable yet nutritionally valuable fruit, through the development and optimization of active edible coatings (ECs). The synergy between cassava starch (Manihot esculenta Crantz) and lemon verbena essential oil (Aloysia citrodora), both bioactive components, was investigated for the formulation of protective coatings. A 2² factorial design explored the impact of cassava starch concentrations (8% and 10% w/v) and lemon verbena essential oil (LVEO) (1% and 3% v/v) on the sensory acceptability of coated cape gooseberries. Through binomial logistic regression analysis, it was determined that the formulation with 10% cassava starch and 3% LVEO (T4) exhibited significantly superior sensory acceptability, optimizing the perception of color, odor, flavor, texture, and overall appearance. This optimized formulation (T4) demonstrated a significant improvement in extending the shelf life of cape gooseberries up to 27 days at 10 °C, which is comparable to or exceeds values reported in previous studies on starch–based coatings in similar fruits (e.g., 15–21 days depending on formulation and storage conditions). This performance also exceeded the storage periods observed at 6 °C (6 days) and 8 °C (20 days). Physicochemical analyses revealed remarkable stability of pH and titratable acidity, as well as effective control of moisture loss and the maturity index, even at higher temperatures. Crucially, T4 exhibited superior antimicrobial activity, with a significant reduction in molds, yeasts, and total aerobes, particularly at 10 °C, suggesting an optimal synergistic interaction between the coating and the LVEO under slightly warmer storage conditions. These findings contribute to the advancement of sustainable preservation strategies of cape gooseberries, offering a sustainable solution that reconciles efficient shelf-life extension with consumer acceptability and optimizes storage conditions, with significant implications for reducing food waste and enhancing the global marketability of this fruit. Full article

Castanea henryi is one of the important fruit tree species cultivated in the mountainous regions of China. Castanea henryi is a starch-accumulating type. As an intracellular substance, starch plays an important role in maintaining cell turgor and sustaining fruit firmness. Although starch metabolism has been extensively studied in model and fruit crops, its regulatory mechanisms in Castanea henryi remain poorly understood. This review synthesizes recent advances in starch physicochemical properties, metabolic pathways, and regulatory mechanisms during fruit development, seed germination, and postharvest storage. Current knowledge gaps, including limited molecular characterization and gene functional analysis in Castanea henryi, are highlighted. This review provides a framework for future research and breeding strategies aimed at improving fruit quality and storage performance. Full article