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Keywords = freshwater use impact

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19 pages, 3457 KiB  
Article
Transcriptome Analysis Revealed the Immune and Metabolic Responses of Grass Carp (Ctenopharyngodon idellus) Under Acute Salinity Stress
by Leshan Ruan, Baocan Wei, Yanlin Liu, Rongfei Mu, Huang Li and Shina Wei
Fishes 2025, 10(8), 380; https://doi.org/10.3390/fishes10080380 - 5 Aug 2025
Abstract
Freshwater salinization, an escalating global environmental stressor, poses a significant threat to freshwater biodiversity, including fish communities. This study investigates the grass carp (Ctenopharyngodon idellus), a species with the highest aquaculture output in China, to elucidate the molecular underpinnings of its [...] Read more.
Freshwater salinization, an escalating global environmental stressor, poses a significant threat to freshwater biodiversity, including fish communities. This study investigates the grass carp (Ctenopharyngodon idellus), a species with the highest aquaculture output in China, to elucidate the molecular underpinnings of its physiological adaptations to fluctuating salinity gradients. We used high-throughput mRNA sequencing and differential gene expression profiling to analyze transcriptional dynamics in intestinal and kidney tissues of grass carp exposed to heterogeneous salinity stressors. Concurrent serum biochemical analyses showed salinity stress significantly increased Na+, Cl, and osmolarity, while decreasing lactate and glucose. Salinity stress exerted a profound impact on the global transcriptomic landscape of grass carp. A substantial number of co-regulated differentially expressed genes (DEGs) in kidney and intestinal tissues were enriched in immune and metabolic pathways. Specifically, genes associated with antigen processing and presentation (e.g., cd4-1, calr3b) and apoptosis (e.g., caspase17, pik3ca) exhibited upregulated expression, whereas genes involved in gluconeogenesis/glycolysis (e.g., hk2, pck2) were downregulated. KEGG pathway enrichment analyses revealed that metabolic and cellular structural pathways were predominantly enriched in intestinal tissues, while kidney tissues showed preferential enrichment of immune and apoptotic pathways. Rigorous validation of RNA-seq data via qPCR confirmed the robustness and cross-platform consistency of the findings. This study investigated the core transcriptional and physiological mechanisms regulating grass carp’s response to salinity stress, providing a theoretical foundation for research into grass carp’s resistance to salinity stress and the development of salt-tolerant varieties. Full article
(This article belongs to the Special Issue Adaptation and Response of Fish to Environmental Changes)
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31 pages, 5440 KiB  
Article
Canals, Contaminants, and Connections: Exploring the Urban Exposome in a Tropical River System
by Alan D. Ziegler, Theodora H. Y. Lee, Khajornkiat Srinuansom, Teppitag Boonta, Jongkon Promya and Richard D. Webster
Urban Sci. 2025, 9(8), 302; https://doi.org/10.3390/urbansci9080302 - 4 Aug 2025
Abstract
Emerging and persistent contaminants (EPCs) were detected at high concentrations in Chiang Mai’s Mae Kha Canal, identifying urban waterways as important sources of pollution in the Ping River system in northern Thailand. Maximum levels of metformin (20,000 ng/L), fexofenadine (15,900 ng/L), gabapentin (12,300 [...] Read more.
Emerging and persistent contaminants (EPCs) were detected at high concentrations in Chiang Mai’s Mae Kha Canal, identifying urban waterways as important sources of pollution in the Ping River system in northern Thailand. Maximum levels of metformin (20,000 ng/L), fexofenadine (15,900 ng/L), gabapentin (12,300 ng/L), sucralose (38,000 ng/L), and acesulfame (23,000 ng/L) point to inadequately treated wastewater as a plausible contributor. Downstream enrichment patterns relative to upstream sites highlight the cumulative impact of urban runoff. Five compounds—acesulfame, gemfibrozil, fexofenadine, TBEP, and caffeine—consistently emerged as reliable tracers of urban wastewater, forming a distinct chemical fingerprint of the riverine exposome. Median EPC concentrations were highest in Mae Kha, lower in other urban canals, and declined with distance from the city, reflecting spatial gradients in urban density and pollution intensity. Although most detected concentrations fell below predicted no-effect thresholds, ibuprofen frequently approached or exceeded ecotoxicological benchmarks and may represent a compound of ecological concern. Non-targeted analysis revealed a broader “chemical cocktail” of unregulated substances—illustrating a witches’ brew of pollution that likely escapes standard monitoring efforts. These findings demonstrate the utility of wide-scope surveillance for identifying key compounds, contamination hotspots, and spatial gradients in mixed-use watersheds. They also highlight the need for integrated, long-term monitoring strategies that address diffuse, compound mixtures to safeguard freshwater ecosystems in rapidly urbanizing regions. Full article
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28 pages, 1185 KiB  
Review
A Review of Water Quality Forecasting Models for Freshwater Lentic Ecosystems
by Jovheiry Christopher García-Guerrero, José M. Álvarez-Alvarado, Roberto Valentín Carrillo-Serrano, Viviana Palos-Barba and Juvenal Rodríguez-Reséndiz
Water 2025, 17(15), 2312; https://doi.org/10.3390/w17152312 - 4 Aug 2025
Viewed by 52
Abstract
Water quality (WQ) monitoring is critical for Mexico and the world due to water pollution and scarcity problems in recent years. In this article, a systematic review was conducted considering only forecasting models focused on lentic freshwater bodies (to specialize the analysis of [...] Read more.
Water quality (WQ) monitoring is critical for Mexico and the world due to water pollution and scarcity problems in recent years. In this article, a systematic review was conducted considering only forecasting models focused on lentic freshwater bodies (to specialize the analysis of variables, problems, considerations, etc.) from 2019 to 2025 (to ensure the inclusion of the most relevant and new studies). This review analyzes 52 articles focused on the monitoring place, predictors, forecasted variables, configuration of each forecasting model, results with or without multiple forecast horizons, monitoring conditions, forecasting horizon, data availability, and model replicability. Our review shows that the main models documented used to predict WQ are based on machine learning (where RFs are the most used), AI (where ANNs are the most used and LSTM-based architectures are the most implemented), and statistical methods (where MLR is the most used). The principal forecasted WQ variables are Chl-α, DO, and TP. In comparison, the most used predictors are TP, temperature, and Chl-α. Furthermore, only 10 articles have made their databases available, and nine articles share the configuration of their models. Future research should investigate the real impact of data (quantity and inputs) variation in forecasting values for multiple forecast horizons. Full article
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33 pages, 1166 KiB  
Article
Evaluating Freshwater, Desalinated Water, and Treated Brine as Water Feed for Hydrogen Production in Arid Regions
by Hamad Ahmed Al-Ali and Koji Tokimatsu
Energies 2025, 18(15), 4085; https://doi.org/10.3390/en18154085 - 1 Aug 2025
Viewed by 113
Abstract
Hydrogen production is increasingly vital for global decarbonization but remains a water- and energy-intensive process, especially in arid regions. Despite growing attention to its climate benefits, limited research has addressed the environmental impacts of water sourcing. This study employs a life cycle assessment [...] Read more.
Hydrogen production is increasingly vital for global decarbonization but remains a water- and energy-intensive process, especially in arid regions. Despite growing attention to its climate benefits, limited research has addressed the environmental impacts of water sourcing. This study employs a life cycle assessment (LCA) approach to evaluate three water supply strategies for hydrogen production: (1) seawater desalination without brine treatment (BT), (2) desalination with partial BT, and (3) freshwater purification. Scenarios are modeled for the United Arab Emirates (UAE), Australia, and Spain, representing diverse electricity mixes and water stress conditions. Both electrolysis and steam methane reforming (SMR) are evaluated as hydrogen production methods. Results show that desalination scenarios contribute substantially to human health and ecosystem impacts due to high energy use and brine discharge. Although partial BT aims to reduce direct marine discharge impacts, its substantial energy demand can offset these benefits by increasing other environmental burdens, such as marine eutrophication, especially in regions reliant on carbon-intensive electricity grids. Freshwater scenarios offer lower environmental impact overall but raise water availability concerns. Across all regions, feedwater for SMR shows nearly 50% lower impacts than for electrolysis. This study focuses solely on the environmental impacts associated with water sourcing and treatment for hydrogen production, excluding the downstream impacts of the hydrogen generation process itself. This study highlights the trade-offs between water sourcing, brine treatment, and freshwater purification for hydrogen production, offering insights for optimizing sustainable hydrogen systems in water-stressed regions. Full article
(This article belongs to the Special Issue Advances in Hydrogen Production in Renewable Energy Systems)
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6 pages, 575 KiB  
Proceeding Paper
Analysing Aquatic Invertebrate Health in Terms of Artificial Light at Night
by Farhan Jamil and Chayan Munshi
Biol. Life Sci. Forum 2025, 45(1), 3; https://doi.org/10.3390/blsf2025045003 - 1 Aug 2025
Viewed by 175
Abstract
Artificial Light at Night (ALAN) is a recent issue of concern for researchers primarily working on the anthropogenic impacts on animal and ecosystem health. Our concern is associated with the ALAN exposure to an aquatic ecosystem by disrupting the natural dark–light cycle, which [...] Read more.
Artificial Light at Night (ALAN) is a recent issue of concern for researchers primarily working on the anthropogenic impacts on animal and ecosystem health. Our concern is associated with the ALAN exposure to an aquatic ecosystem by disrupting the natural dark–light cycle, which is essential for maintaining the overall health of the ecosystem and its inhabitants. In this study, we have attempted to understand the adverse consequences of ALAN in inducing neuro-behavioural stress in a freshwater prawn species (aquatic arthropod) Macrobrachium lamarrei by considering grooming behaviour, a well-established indicator of neurological stress in animals. Our results show that continuous ALAN exposure (for seven days) can increase collective grooming activity in Macrobrachium lamarrei over time. In our experiment, we have used two intensities of ALAN (50 and 120 lux). Although the response (in terms collective grooming) to both intensities are apparently different, our fundamental hypothesis is confirmed, where it is evident that prolonged light exposure can induce an elevation in cumulative grooming performances in a freshwater prawn population. Full article
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23 pages, 819 KiB  
Article
The Nexus Between Economic Growth and Water Stress in Morocco: Empirical Evidence Based on ARDL Model
by Mariam El Haddadi, Hamida Lahjouji and Mohamed Tabaa
Sustainability 2025, 17(15), 6990; https://doi.org/10.3390/su17156990 - 1 Aug 2025
Viewed by 226
Abstract
Morocco is facing a situation of alarming water stress, aggravated by climate change, overexploitation of resources, and unequal distribution of water, placing the country among the most vulnerable to water scarcity in the MENA region. This study aims to investigate the dynamic relationship [...] Read more.
Morocco is facing a situation of alarming water stress, aggravated by climate change, overexploitation of resources, and unequal distribution of water, placing the country among the most vulnerable to water scarcity in the MENA region. This study aims to investigate the dynamic relationship between economic growth and water stress in Morocco while highlighting the importance of integrated water management and adaptive economic policies to enhance resilience to water scarcity. A mixed methodology, integrating both qualitative and quantitative methods, was adopted to overview the economic–environmental Moroccan context, and to empirically analyze the GDP (gross domestic product) and water stress in Morocco over the period 1975–2021 using an Autoregressive Distributed Lag (ARDL) approach. The empirical analysis is based on annual data sourced from the World Bank and FAO databases for GDP, agricultural value added, renewable internal freshwater resources, and water productivity. The results suggest that water productivity has a significant positive effect on economic growth, while the impacts of agricultural value added and renewable water resources are less significant and vary depending on the model specification. Diagnostic tests confirm the reliability of the ARDL model; however, the presence of outliers in certain years reflects the influence of exogenous shocks, such as severe droughts or policy changes, on the Moroccan economy. The key contribution of this study lies in the fact that it is the first to analyze the intrinsic link between economic growth and the environmental aspect of water in Morocco. According to our findings, it is imperative to continuously improve water productivity and adopt adaptive management, rooted in science and innovation, in order to ensure water security and support the sustainable economic development of Morocco. Full article
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15 pages, 3267 KiB  
Article
Monitoring and Analyzing Aquatic Vegetation Using Sentinel-2 Imagery Time Series: A Case Study in Chimaditida Shallow Lake in Greece
by Maria Kofidou and Vasilios Ampas
Limnol. Rev. 2025, 25(3), 35; https://doi.org/10.3390/limnolrev25030035 - 1 Aug 2025
Viewed by 122
Abstract
Aquatic vegetation plays a crucial role in freshwater ecosystems by providing habitats, regulating water quality, and supporting biodiversity. This study aims to monitor and analyze the dynamics of aquatic vegetation in Chimaditida Shallow Lake, Greece, using Sentinel-2 satellite imagery, with validation from field [...] Read more.
Aquatic vegetation plays a crucial role in freshwater ecosystems by providing habitats, regulating water quality, and supporting biodiversity. This study aims to monitor and analyze the dynamics of aquatic vegetation in Chimaditida Shallow Lake, Greece, using Sentinel-2 satellite imagery, with validation from field measurements. Data processing was performed using Google Earth Engine and QGIS. The study focuses on discriminating and mapping two classes of aquatic surface conditions: areas covered with Floating and Emergent Aquatic Vegetation and open water, covering all seasons from 1 March 2024, to 28 February 2025. Spectral bands such as B04 (red), B08 (near infrared), B03 (green), and B11 (shortwave infrared) were used, along with indices like the Modified Normalized Difference Water Index and Normalized Difference Vegetation Index. The classification was enhanced using Otsu’s thresholding technique to distinguish accurately between Floating and Emergent Aquatic Vegetation and open water. Seasonal fluctuations were observed, with significant peaks in vegetation growth during the summer and autumn months, including a peak coverage of 2.08 km2 on 9 September 2024 and a low of 0.00068 km2 on 28 December 2024. These variations correspond to the seasonal growth patterns of Floating and Emergent Aquatic Vegetation, driven by temperature and nutrient availability. The study achieved a high overall classification accuracy of 89.31%, with producer accuracy for Floating and Emergent Aquatic Vegetation at 97.42% and user accuracy at 95.38%. Validation with Unmanned Aerial Vehicle-based aerial surveys showed a strong correlation (R2 = 0.88) between satellite-derived and field data, underscoring the reliability of Sentinel-2 for aquatic vegetation monitoring. Findings highlight the potential of satellite-based remote sensing to monitor vegetation health and dynamics, offering valuable insights for the management and conservation of freshwater ecosystems. The results are particularly useful for governmental authorities and natural park administrations, enabling near-real-time monitoring to mitigate the impacts of overgrowth on water quality, biodiversity, and ecosystem services. This methodology provides a cost-effective alternative for long-term environmental monitoring, especially in regions where traditional methods are impractical or costly. Full article
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7 pages, 1048 KiB  
Data Descriptor
Dataset of Morphometry and Metal Concentrations in Coptodon rendalli and Oreochromis mossambicus from the Shongweni Dam, South Africa
by Smangele Ncayiyana, Neo Mashila Maleka and Jeffrey Lebepe
Data 2025, 10(8), 124; https://doi.org/10.3390/data10080124 - 1 Aug 2025
Viewed by 186
Abstract
The uMlazi River receives effluents from wastewater work before feeding the Shongweni Dam. However, local communities are consuming fish from this dam for protein supplements. This study was undertaken to investigate the metal concentrations in the water and sediment, the general health of [...] Read more.
The uMlazi River receives effluents from wastewater work before feeding the Shongweni Dam. However, local communities are consuming fish from this dam for protein supplements. This study was undertaken to investigate the metal concentrations in the water and sediment, the general health of Coptodon rendalli and Oreochromis mossambicus, and metal bioaccumulation. Sampling was conducted during the dry (July–August) and wet seasons (November and December) in 2021. Water was sampled using acid-pre-treated sampling bottles, whereas sediment was collected using the Van Veen grab at the inflow, middle, and dam wall. Fish were collected, and their tissues were digested using aqua regia. Metal concentrations were measured using inductively coupled plasma optical emission spectroscopy (ICP-OES). This data manuscript reports the physical parameters of the water and concentrations of antimony, arsenic, cadmium, copper, iron, manganese, lead, selenium, and strontium in the water and sediment from the Shongweni Dam. Moreover, the fish morphometric data and metal concentrations observed in the muscle are also presented. This data could be used as baseline information on metal concentrations in the Shongweni Dam. Moreover, it provides insight into the potential impact of wastewater effluents on metal increases in freshwater bodies. Full article
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24 pages, 5968 KiB  
Article
Life Cycle Assessment of a Digital Tool for Reducing Environmental Burdens in the European Milk Supply Chain
by Yuan Zhang, Junzhang Wu, Haida Wasim, Doris Yicun Wu, Filippo Zuliani and Alessandro Manzardo
Appl. Sci. 2025, 15(15), 8506; https://doi.org/10.3390/app15158506 (registering DOI) - 31 Jul 2025
Viewed by 109
Abstract
Food loss and waste from the European Union’s dairy supply chain, particularly in the management of fresh milk, imposes significant environmental burdens. This study demonstrates that implementing Radio Frequency Identification (RFID)-enabled digital decision-support tools can substantially reduce these impacts across the region. A [...] Read more.
Food loss and waste from the European Union’s dairy supply chain, particularly in the management of fresh milk, imposes significant environmental burdens. This study demonstrates that implementing Radio Frequency Identification (RFID)-enabled digital decision-support tools can substantially reduce these impacts across the region. A cradle-to-grave life cycle assessment (LCA) was used to quantify both the additional environmental burdens from RFID (tag production, usage, and disposal) and the avoided burdens due to reduced milk losses in the farm, processing, and distribution stages. Within the EU’s fresh milk supply chain, the implementation of digital tools could result in annual net reductions of up to 80,000 tonnes of CO2-equivalent greenhouse gas emissions, 81,083 tonnes of PM2.5-equivalent particulate matter, 84,326 tonnes of land use–related carbon deficit, and 80,000 cubic meters of freshwater-equivalent consumption. Spatial analysis indicates that regions with historically high spoilage rates, particularly in Southern and Eastern Europe, see the greatest benefits from RFID enabled digital-decision support tools. These environmental savings are most pronounced during the peak months of milk production. Overall, the study demonstrates that despite the environmental footprint of RFID systems, their integration into the EU’S dairy supply chain enhances transparency, reduces waste, and improves resource efficiency—supporting their strategic value. Full article
(This article belongs to the Special Issue Artificial Intelligence and Numerical Simulation in Food Engineering)
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20 pages, 2497 KiB  
Article
Sustainable Solar Desalination: Experimental Predictive Control with Integrated LCA and Techno-Economic Evaluation
by Mishal Alsehli
Processes 2025, 13(8), 2364; https://doi.org/10.3390/pr13082364 - 25 Jul 2025
Viewed by 299
Abstract
This study experimentally validates a solar-thermal desalination system equipped with predictive feedwater control guided by real-time solar forecasting. Unlike conventional systems that react to temperature changes, the proposed approach proactively adjusts feedwater flow in anticipation of solar variability. To assess environmental and financial [...] Read more.
This study experimentally validates a solar-thermal desalination system equipped with predictive feedwater control guided by real-time solar forecasting. Unlike conventional systems that react to temperature changes, the proposed approach proactively adjusts feedwater flow in anticipation of solar variability. To assess environmental and financial sustainability, the study integrates this control logic with a full Life Cycle Assessment (LCA) and Techno-Economic Analysis (TEA). Field testing in a high-temperature, arid region demonstrated strong performance, achieving a Global Warming Potential (GWP) of 1.80 kg CO2-eq/m3 and a Levelized Cost of Water (LCOW) of $0.88/m3. Environmental impacts were quantified using OpenLCA and ecoinvent datasets, covering climate change, acidification, and eutrophication categories. The TEA confirmed economic feasibility, reporting a positive Net Present Value (NPV) and an Internal Rate of Return (IRR) exceeding 11.5% over a 20-year lifespan. Sensitivity analysis showed that forecast precision and TES design strongly influence both environmental and economic outcomes. The integration of intelligent control with simplified thermal storage offers a scalable, cost-effective solution for off-grid freshwater production in solar-rich regions. Full article
(This article belongs to the Section Sustainable Processes)
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16 pages, 2096 KiB  
Article
Environmental Antidepressants Disrupt Metabolic Pathways in Spirostomum ambiguum and Daphnia magna: Insights from LC-MS-Based Metabolomics
by Artur Jędreas, Sylwia Michorowska, Agata Drobniewska and Joanna Giebułtowicz
Molecules 2025, 30(14), 2952; https://doi.org/10.3390/molecules30142952 - 13 Jul 2025
Viewed by 464
Abstract
Pharmaceuticals such as fluoxetine, paroxetine, sertraline, and mianserin occur in aquatic environments at low yet persistent concentrations due to their incomplete removal in wastewater treatment plants. Although frequently detected, these neuroactive compounds remain underrepresented in ecotoxicological assessments. Given their pharmacodynamic potency, environmentally relevant [...] Read more.
Pharmaceuticals such as fluoxetine, paroxetine, sertraline, and mianserin occur in aquatic environments at low yet persistent concentrations due to their incomplete removal in wastewater treatment plants. Although frequently detected, these neuroactive compounds remain underrepresented in ecotoxicological assessments. Given their pharmacodynamic potency, environmentally relevant concentrations may induce sublethal effects in non-target organisms. In this study, we applied untargeted LC-MS-based metabolomics to investigate the sublethal effects of four widely used antidepressants—paroxetine, sertraline, fluoxetine (SSRIs), and mianserin (TeCA)—on two ecologically relevant freshwater invertebrates: S. ambiguum and D. magna. Organisms were individually exposed to each compound for 48 h at a concentration of 100 µg/L and 25 µg/L, respectively. Untargeted metabolomics captured the sublethal biochemical effects of these antidepressants, revealing both shared disruptions—e.g., in glycerophospholipid metabolism and cysteine and methionine metabolism—and species-specific responses. More pronounced pathway changes observed in D. magna suggest interspecies differences in metabolic capacity or xenobiotic processing mechanisms between taxa. Among the four antidepressants tested, sertraline in D. magna and fluoxetine in S. ambiguum exerted the most extensive metabolomic perturbations, as evidenced by the highest number and pathway impact scores. In D. magna, fluoxetine and mianserin produced similar metabolic profiles, largely overlapping with those of sertraline, whereas paroxetine affected only a single pathway, indicating minimal impact. In S. ambiguum, paroxetine and mianserin elicited comparable responses, also overlapping with those of fluoxetine, while sertraline triggered the fewest changes. These results suggest both compound-specific effects and a conserved metabolic response pattern among the antidepressants used. They also underscore the considerable potential of metabolomics as a powerful and sensitive tool for ecotoxicological risk assessments, particularly when applied across multiple model organisms to capture interspecies variations. However, further research is essential to identify which specific pathway disruptions are most predictive of adverse effects on organismal health. Full article
(This article belongs to the Special Issue Advances in the Mass Spectrometry of Chemical and Biological Samples)
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15 pages, 2181 KiB  
Article
The Impact of Shifts in Both Precipitation Pattern and Temperature Changes on River Discharge in Central Japan
by Bing Zhang, Jingyan Han, Jianbo Liu and Yong Zhao
Hydrology 2025, 12(7), 187; https://doi.org/10.3390/hydrology12070187 - 9 Jul 2025
Viewed by 467
Abstract
Rivers play a crucial role in the hydrological cycle and serve as essential freshwater resources for both human populations and ecosystems. Climate change significantly alters precipitation patterns and river discharge variability. However, the impact of precipitation patterns (rainfall and snowfall) and air temperature [...] Read more.
Rivers play a crucial role in the hydrological cycle and serve as essential freshwater resources for both human populations and ecosystems. Climate change significantly alters precipitation patterns and river discharge variability. However, the impact of precipitation patterns (rainfall and snowfall) and air temperature on river discharge in coastal zones remains inadequately understood. This study focused on Toyama Prefecture, located along the Sea of Japan, as a representative coastal area. We analyzed over 30 years of datasets, including air temperature, precipitation, snowfall, and river discharge, to assess the effects of climate change on river discharge. Trends in hydroclimatic datasets were assessed using the rescaled adjusted partial sums (RAPS) method and the Mann–Kendall (MK) non-parametric test. Furthermore, a correlation analysis and the Structural Equation Model (SEM) were applied to construct a relationship between precipitation, temperature, and river discharge. Our findings indicated a significant increase in air temperature at a rate of 0.2 °C per decade, with notable warming observed in late winter (January and February) and early spring (March). The average river fluxes for the Jinzu, Oyabe, Kurobe, Shou, and Joganji rivers were 182.52 m3/s, 60.37 m3/s, 41.40 m3/s, 38.33 m3/s, and 18.72 m3/s, respectively. The tipping point for snowfall decline occurred in 1992, marked by an obvious decrease in snowfall depth. The SEM showed that, although rainfall dominated the changes in river discharge (loading = 0.94), the transition from solid (snow) to liquid (rain) precipitation may alter the river discharge regime. The percentage of flood occurrence increased from 19% (1940–1992) to 41% (1993–2020). These changes highlight the urgent need to raise awareness about the impacts of climate change on river floods and freshwater resources in global coastal regions. Full article
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14 pages, 6120 KiB  
Article
Drones and Deep Learning for Detecting Fish Carcasses During Fish Kills
by Edna G. Fernandez-Figueroa, Stephanie R. Rogers and Dinesh Neupane
Drones 2025, 9(7), 482; https://doi.org/10.3390/drones9070482 - 8 Jul 2025
Viewed by 400
Abstract
Fish kills are sudden mass mortalities that occur in freshwater and marine systems worldwide. Fish kill surveys are essential for assessing the ecological and economic impacts of fish kill events, but are often labor-intensive, time-consuming, and spatially limited. This study aims to address [...] Read more.
Fish kills are sudden mass mortalities that occur in freshwater and marine systems worldwide. Fish kill surveys are essential for assessing the ecological and economic impacts of fish kill events, but are often labor-intensive, time-consuming, and spatially limited. This study aims to address these challenges by exploring the application of unoccupied aerial systems (or drones) and deep learning techniques for coastal fish carcass detection. Seven flights were conducted using a DJI Phantom 4 RGB quadcopter to monitor three sites with different substrates (i.e., sand, rock, shored Sargassum). Orthomosaics generated from drone imagery were useful for detecting carcasses washed ashore, but not floating or submerged carcasses. Single shot multibox detection (SSD) with a ResNet50-based model demonstrated high detection accuracy, with a mean average precision (mAP) of 0.77 and a mean average recall (mAR) of 0.81. The model had slightly higher average precision (AP) when detecting large objects (>42.24 cm long, AP = 0.90) compared to small objects (≤14.08 cm long, AP = 0.77) because smaller objects are harder to recognize and require more contextual reasoning. The results suggest a strong potential future application of these tools for rapid fish kill response and automatic enumeration and characterization of fish carcasses. Full article
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19 pages, 9996 KiB  
Article
Plant Traits in Spring and Winter Canola Genotypes Under Salinity
by Rajan Shrestha, Qingwu Xue, Andrea Leiva Soto, Girisha Ganjegunte, Santosh Subhash Palmate, Vijayasatya N. Chaganti, Saurav Kumar, April L. Ulery and Samuel Zapata
Agronomy 2025, 15(7), 1657; https://doi.org/10.3390/agronomy15071657 - 8 Jul 2025
Viewed by 381
Abstract
Concerning rising salinity and declining freshwater supply in the U.S. Southern Great Plains, alternative crop production choices using marginal saline irrigation water are irresistible. The study investigated plant traits related to salt tolerance in greenhouse canola (Brassica napus L.) in 2022 and [...] Read more.
Concerning rising salinity and declining freshwater supply in the U.S. Southern Great Plains, alternative crop production choices using marginal saline irrigation water are irresistible. The study investigated plant traits related to salt tolerance in greenhouse canola (Brassica napus L.) in 2022 and 2023. Spring and winter canola, including ten genotypes each, were evaluated at six salinity levels (0; control, 2, 4, 6, 8, and 8 dS m−1 EC). Plant height, stem mass, leaf area, and specific leaf area (SLA) showed a negative linear response, while quadratic relationships were observed in biomass and leaf mass with increased salinity levels. Substantial negative salinity impacts on plant traits occurred at ≥6 dS m−1 EC (p ≤ 0.01) except for SLA. Overall, winter canola genotypes: Athena, Ericka, CP320WRR, CP115W, and CP225WRR, and spring genotypes: Empire, Monarch, Profit, and Westar, were relatively more salt-tolerant than others. Spring canola showed greater salinity tolerance than winter canola. Salinity stress resulted in differential responses of greater leaf mass in winter canola but more efficient leaf area production in spring canola. SLA and stem mass were highly correlated with most parameters. Findings indicate SLA and stem mass are potential salt tolerance traits in canola and warrant further investigations and validation. Full article
(This article belongs to the Section Crop Breeding and Genetics)
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15 pages, 528 KiB  
Review
Water Monitoring Practices 2.0—Water Fleas as Key Species in Ecotoxicology and Risk Assessment
by Anne Leung, Emma Rowan, Flavia Melati Chiappara and Konstantinos Grintzalis
Limnol. Rev. 2025, 25(3), 30; https://doi.org/10.3390/limnolrev25030030 - 2 Jul 2025
Viewed by 302
Abstract
Humanity faces the great challenges arising from pollution and climate change which evidently lead to the irreversible effects observed on the planet. It is now more important than ever to monitor and safeguard the ecosystem as it has been highlighted by governments and [...] Read more.
Humanity faces the great challenges arising from pollution and climate change which evidently lead to the irreversible effects observed on the planet. It is now more important than ever to monitor and safeguard the ecosystem as it has been highlighted by governments and scientists. Conventional approaches for water pollution rely on the detection of chemicals in the environment. However, these descriptive observations when compared against water quality standards used as metrics for pollution are unable to predict pollution early or capture the extent of its impact. This weakness is reflected in the legislation and the thresholds for emerging pollutants such as pharmaceuticals and nanomaterials. To bridge the gap and to understand the underlying mechanisms for toxicity, research in the field of molecular ecotoxicology shifts more and more towards the integration of model systems, in silico approaches and molecular information as endpoints. Focusing on the freshwater ecosystem, daphnids are key species employed in risk assessment which are characterised as highly responsive to pollutants and physical stressors. The translation of molecular information describing the physiology of these organisms provides novel and sensitive metrics for pollution assessment. Full article
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