Search Results (26,684)

Background/Objectives: Prostate cancer is the most commonly diagnosed cancer in men and a leading cause of cancer-related mortality, highlighting the need for delivery systems capable of efficiently transporting both chemotherapeutic drugs and therapeutic genes to tumor cells. Generation-3 diaminobutyric polypropylenimine (DAB) dendrimers display low toxicity, high drug loading capacity and efficient gene delivery, and can be engineered as camptothecin-bearing PEGylated carriers complexed with plasmid DNA. The aim of this study was to compare microfluidic processing with conventional hand mixing for the preparation of camptothecin-bearing PEGylated DAB dendriplexes and to evaluate the impact of formulation methods and microfluidic parameters on their physicochemical properties, cellular uptake and gene expression in prostate cancer cells. Methods: Camptothecin-bearing PEGylated DAB dendrimers were synthesized and complexed with plasmid DNA to form dendriplexes. Formulations were prepared either by microfluidics, using different total flow rates and aqueous: organic flow rate ratios, or by conventional hand mixing. The resulting dendriplexes were characterized for DNA condensation, particle size, polydispersity index and zeta potential. Morphology was assessed by transmission electron microscopy. Cellular uptake of fluorescein-labelled DNA and β-galactosidase reporter gene expression were evaluated in PC3-Luc and DU145 prostate cancer cells. Results: Both microfluidic and hand-mixed methods produced stable, nanosized, positively charged dendriplexes with efficient and sustained DNA condensation (more than 99% over 24 h). Microfluidic processing, particularly at an aqueous: organic flow rate ratio of 3:1, yielded dendriplexes with hydrodynamic diameters and zeta potentials comparable to or slightly improved over hand-mixed formulations. These microfluidic conditions significantly enhanced cellular uptake in both PC3-Luc and DU145 cells. In PC3-Luc cells, this translated into β-galactosidase expression levels comparable to hand-mixed dendriplexes and higher than naked DNA, whereas in DU145 cells, transfection efficiencies remained modest for all formulations despite increased uptake. Conclusions: Microfluidic processing enables the reproducible and scalable preparation of camptothecin-bearing PEGylated DAB dendriplexes with tunable physicochemical properties. Under selected conditions, in vitro cellular uptake and gene expression were comparable to conventional hand mixing, supporting microfluidics as a robust alternative platform for the manufacture of dendrimer-based systems for combined chemo–gene delivery in prostate cancer. Full article

An analysis is presented for the steady thermophoresis and photophoresis of a homogeneous dispersion of identical aerosol spheres of typical physical properties and surface characteristics. The analysis assumes a moderately small Knudsen number (less than about 0.1), such that the gas motion lies within the slip-flow regime, including thermal creep, temperature jump, thermal stress slip, and frictional slip at the particle surfaces. Under conditions of low Peclet and Reynolds numbers, the coupled momentum and energy equations are analytically solved using a unit cell approach that explicitly incorporates interparticle interactions. Closed-form expressions are derived for the mean particle migration velocities in both thermophoresis driven by a uniform temperature gradient and photophoresis induced by an incident radiation field. The results reveal that the normalized particle velocities, referenced to those of an isolated particle, generally decrease with increasing particle volume fraction, though exceptions occur for thermophoresis. While thermal stress slip and thermal creep exert no influence on the normalized thermophoretic velocity, they markedly affect the normalized photophoretic velocity, which rises with the thermal stress slip to the thermal creep coefficient ratio. For both phenomena, the normalized migration velocities increase monotonically with the particle-to-fluid thermal conductivity ratio. Full article

Background: Neuroblastoma (NB) is the most common extracranial paediatric solid cancer, with a 50% survival rate for high-risk patients. Circulating tumour cells (CTCs) are malignant cells shed by the primary tumour and metastatic sites that circulate in the bloodstream. CTCs form clusters with themselves (homotypic) or other cell types (heterotypic). Objectives: To use previously generated ImageStreamX Imaging Flow Cytometer data from blood samples from 24 patients across all NB risk groups, to examine for the presence of CTC clusters. Methods: Immunofluorescence and brightfield morphology were used to identify clusters followed by analysis using IDEAS image analysis software. Results: The mean number of clusters detected per sample was 87 (range, 0–725). Of the clusters detected, 1967/2094 (93.9%) were heterotypic and only 127/2094 (6.1%) were homotypic and found in 6/24 patients. Interestingly, in 3/24 patients, at least one cluster (median, 2 and range, 1–18) was found, but no single CTCs were detected. Clusters mostly comprised two cells (62.8%), with the maximum number of cells in homotypic and heterotypic clusters being four and eight, respectively. Conclusions: These results highlight that imaging flow cytometry can be used to detect and characterise CTC clusters in peripheral blood samples from NB patients, leading to further research exploring the composition and role of CTC clusters in NB metastasis. Full article

The northern segment of the Great Xing’an Range, northeastern China, hosts a previously unrecognized near-E–W-trending rhyolite belt in the Tulihe area. We conducted systematic geochronological and geochemical investigations to constrain its formation age, petrogenesis, and regional tectonic significance. Field investigation, petrographic observation, and zircon laser ablation–inductively coupled plasma–mass spectrometry (LA-ICP-MS) U–Pb dating indicate that the rhyolite belt was formed during the Early Cretaceous, with emplacement ages directly determined from three samples ranging from 143.8 to 131.5 Ma. Geochemically, the rhyolites yielded high SiO₂ contents (74.44–75.88 wt.%), high total alkalis (K₂O + Na₂O = 8.50–8.99 wt.%), and low MgO contents (0.16–0.55 wt.%). They displayed strong enrichment in light rare earth elements and depletion in high field strength elements, weakly negative Eu anomalies, A/CNK ratios near unity, and relatively high Nb/Ta ratios. Trace element signatures and incompatible element abundances (Zr + Nb + Ce + Y = 193.2–338.3 × 10⁻⁶) are mostly consistent with highly fractionated I-type volcanic rocks, rather than S-type or M-type affinities. The geochemical data suggest that the rhyolites were mainly generated by partial melting of a medium- to high-K basaltic lower crust, with minor crustal assimilation and limited mantle input. Tectonically, Early Cretaceous magmatism in the northern Great Xing’an Range was governed by flat-slab subduction and subsequent rollback of the Paleo-Pacific (Izanagi) plate, while the local E–W-trending rhyolite belt was controlled by pre-existing faults, reflecting localized post-orogenic extension consistent with regional NE-trending volcanic belts. The northwest-to-southeast younging trend records asthenospheric upwelling and enhanced crust–mantle interaction induced by slab rollback. These results highlight the petrogenetic and tectonic evolution of medium- to high-K magmatism along the NE Asian continental margin and improve our understanding of Mesozoic volcanism in the Great Xing’an Range. Full article

To meet the requirements of next-generation spacecraft thermal protection systems for lightweight materials with high strength, effective thermal insulation, and superior ablation resistance, a novel POSS-modified phenolic aerogel/quartz fiber composite (POSS-PR/QF) was developed using a thiol–ene click reaction combined with a sol–gel process. Covalent incorporation of polyhedral oligomeric silsesquioxanes (POSS) into the phenolic matrix effectively eliminates nanoparticle aggregation and improves interfacial compatibility. As a result, the modified resin is suitable for resin transfer molding (RTM) processes. The resulting composite exhibited an aerogel-like porous structure with enhanced crosslinking density, thermal stability, and oxidation resistance. At 7.5 wt% POSS loading, the composite achieved low density (~0.7 g·cm⁻³) and outstanding mechanical properties, with tensile, flexural, compressive, and interlaminar shear strengths increased by 114%, 79%, 29%, and 104%, respectively. Its thermal conductivity (0.0619 W/(m·K)) and ablation rates were also markedly reduced. Mechanistic studies revealed that POSS undergoes in situ ceramification to form SiO₂ and SiC phases, which create a dense protective barrier. In addition, this ceramification process promotes char graphitization, thereby enhancing oxidation resistance and thermal insulation. This work provides a promising approach for designing lightweight, high-performance, and multifunctional thermal protection materials for aerospace applications. Full article

This article analyzes the Solimene façade (Vietri sul Mare, Campania, Italy, 1952–1955). The survey, already acquired with active and passive sensors, was integrated with close-range photogrammetry of some sections of the main wall. The purpose of the new acquisitions was to generate data to inform a plug-in that, in the latest versions of the Revit software, correlates parametric and procedural environments. The focus of the study was the rationalization of the formal structure of the amphora, the heart of the main façade. Logic and geometric language guide the identification of a possible mathematical relationship aimed at parametrically modifying the model. The logical diagrams, converted into a Grasshopper preview, can be managed through graphical nodes. In the form of flowcharts (visual scripts), the finite sequence of procedural steps has the advantage of managing and modifying, in real time and in a user-friendly manner, the morphometric characteristics of the small “mummarella.” The results identify the morphometric characteristics common to a typological family composed of Vietri amphorae that, in the field of architectural design, uses the typical functions of system families. The goal is to approach sustainable and participatory design solutions by providing functions that can be graphically manipulated from within the software environment. Full article

Endocervical adenocarcinoma is now classified within an etiologic framework based on the presence or absence of high-risk human papillomavirus (HPV) infection. Gastric-type endocervical adenocarcinoma (GAS) is the prototypical HPV-independent subtype, accounting for up to 25% of endocervical adenocarcinomas and showing a particularly high frequency in East Asia. GAS is typically diagnosed at a more advanced stage than usual-type HPV-associated endocervical adenocarcinoma (UEA); exhibits deep stromal and parametrial invasion, lymphovascular space invasion, and a strong propensity for ovarian and peritoneal metastasis; and is associated with markedly worse survival, even in stage I disease. Radiological evaluation is challenging because of diffuse infiltrative growth, prominent mucin production, and frequent underestimation of extra-cervical spread. Histologically, GAS shows gastric-type (pyloric) differentiation, ranging from minimal deviation adenocarcinoma to poorly differentiated forms, and often overlaps with precursor lesions such as atypical lobular endocervical glandular hyperplasia and gastric-type adenocarcinoma in situ. Immunophenotypically, GAS is typically p16-negative, ER/PR-negative, and frequently exhibits mutant-type p53 and expression of gastric markers including MUC6, HIK1083, and claudin 18.2. Recent next-generation sequencing and multi-omics studies have revealed recurrent alterations in TP53, CDKN2A, STK11, KRAS, ARID1A, KMT2D, and homologous recombination-related genes, together with the activation of PI3K/AKT, WNT/β-catenin, TGF-β, and EMT pathways and characteristic metabolic reprogramming. GAS is highly resistant to conventional chemotherapy and radiotherapy, and its current management follows guidelines for squamous and usual-type adenocarcinoma. Emerging data support precision-medicine approaches targeting HER2/HER3, PD-1/PD-L1, and claudin 18.2, and suggest a role for PARP inhibition and other genotype-directed therapies in selected subsets. Given its aggressive biology and rising relative incidence in the HPV-vaccination era, GAS represents a critical unmet need in gynecologic oncology. Future progress hinges on developing reliable diagnostic biomarkers, refining imaging protocols, and validating targeted therapies through international clinical trials. Full article

The escalating housing crisis and the uncontrolled proliferation of informal settlements in the Global South challenge the modernist ideal of the completed architectural object. While ‘Parasitic Architecture’ is conventionally coded as an act of illegal occupation, ‘Incremental Housing’ strategies propose a controlled evolution; however, a theoretical gap exists in defining the morphological mechanics where these two concepts intersect. This study aims to bridge this gap by proposing the concept of ‘Domesticated Parasitism’. Adopting an instrumental case study model, the research analyzes the morphological evolution of the Quinta Monroy housing complex in Chile. To mitigate interpretive bias and ensure analytical objectivity, the visual reading follows a structured coding protocol that categorizes the intervention zones into three distinct layers: (1) Fixed Structural Matrix, (2) Defined Expansion Zones, and (3) User-Generated Infill. Findings from the diachronic analysis comparing the initial state with current saturation levels reveal that the host structure functions as a ‘spatial cage’ that disciplines the growth of user additions. Unlike uncontrolled urban sprawl, the visual evidence confirms that the parasitic additions strictly adhere to the vertical void geometry defined by the architect. The research concludes that the architect’s role transforms from an author of static forms to an enabler, positioning domesticated parasitism as a sustainable spatial grammar for urban densification. Full article

High-quality synthetic text can mitigate annotation scarcity in text classification. However, standard preference optimization often produces samples that are fluent but weakly label-specific. We present Utility-weighted Direct Preference Optimization (U-DPO), a preference-optimization framework for class-conditional synthetic data generation. In U-DPO, a task-specific classifier provides a margin-based external score for each candidate generation, which is combined with an embedding-based internal similarity score to form an overall utility. These utilities are used (i) to mine preference pairs from multiple candidates per class and (ii) to weigh each DPO update by the utility gap between preferred and dispreferred samples. This design encourages the generator to concentrate on learning informative, label-discriminative preference comparisons rather than treating all pairs equally. Across two multiclass scientific-abstract benchmarks (arXiv and WOS-11967), U-DPO consistently improves downstream SciBERT classification accuracy compared with both vanilla synthetic generation and standard DPO fine-tuning, with gains up to 0.88 percentage points on arXiv and 0.83 percentage points on WOS-11967 depending on the generator. An additional GPT-4.5-based evaluation also indicates a higher mean quality score for U-DPO samples with reduced variance. Full article

Background: Influencer marketing has become one of the most effective strategies in digital communication due to its capacity to generate trust, credibility and endorsement within segmented online communities. Within the tourism sector, travel influencers have been progressively integrated as key agents in destination and brand promotion, contributing to both the construction of tourism-related perceptions and travel decision-making. This study aims to analyse how travel influencers communicate and promote tourist destinations, focusing on their profiles, content formats, commercial transparency and audience engagement. Methods: The research is based on a quantitative content analysis of publications by leading Spanish travel influencers identified through the Forbes Best Content Creators 2025 ranking. The observation period covered March to July 2025. Analysis was structured around four analytical blocks comprising 17 variables related to influencer profile, format and content, commercial transparency and ethics, and interaction. Results: The results reveal consistent behavioural patterns associated with gender, destination type and narrative style. Male influencers are more frequently linked to adventure-oriented storytelling and natural landscapes, whereas female influencers tend to emphasise urban and cultural experiences. Short-form video emerges as the dominant format, generating higher interaction levels, while engagement proves to be a more informative indicator of effectiveness than follower count. Conclusions: The findings underscore the importance of prioritising specialisation, narrative coherence, authenticity and transparency when integrating influencers into their communication strategies. Full article

This article reinterprets modern realist drama as a site of secular spirituality, where aesthetic form sustains the sacred under conditions of modern secularity. Employing a phenomenological–theological framework, it integrates Charles Taylor’s account of the secular age, Mircea Eliade’s sacred–profane dialectic and hierophany, and René Girard’s anthropology of sacrifice. Through textual and performance-historical analysis of key works—Ibsen’s A Doll’s House (1879) and An Enemy of the People (1882)—together with Chinese modern drama shaped by Ibsenization, including Hu Shi’s translations, Lu Xun’s critiques, and Cao Yu’s Thunderstorm (1934), the article argues that realist theatre fulfils religious functions in secular culture: revelation as truth-telling, confession as critical self-disclosure, and renewal as ethical transformation. In early twentieth-century China, the encounter between Ibsen’s moral realism and indigenous moral traditions generated a distinctive spiritual humanism, in which theatre assumed ritual and didactic functions traditionally associated with religious practices. Full article

Based on leadership narratives collected between 2022 and 2025, this article examines how Ukrainian non-military organizational and community leaders who have remained in the country during the ongoing war interpret, embody, and enact theological meaning within their lived leadership experience. Drawing on two qualitative datasets—one collected in 2022 (n = 145) and a second in 2025 (n = 79)—the study employs a lived theology approach together with a reflexive thematic analysis to explore how theological meaning emerges organically as stated in leaders’ accounts of suffering, responsibility, presence, and hope. The findings indicate that participants articulated three overarching movements of lived theology: lament, leading to dependence on God; the sensed presence of God, leading to social solidarity and shared responsibility; and hope in God, orienting leaders toward post-war restoration. These movements function not as abstract or institutionally authorized doctrines, but as dynamic theological orientations generated through lived theological reflection as leaders connect their perceptions of God with the realities of wartime life. The study contributes to practical theology by demonstrating how theological reflection arises from concrete leadership practices under conditions of war. It further advances leadership studies by showing how theological sense-making, suffering, and responsibility converge in the lives of ordinary people—leaders and followers alike—forming a shared spiritual orientation that sustains communal life amid war and nurtures hope for post-war renewal. Full article

Cynomorium songaricum is an important medicinal holoparasitic plant in the arid regions of northwestern China. Its extremely low seed germination rate relies on chemical signals released from the rhizosphere of specific host plants. This study aimed to elucidate the chemical basis of host recognition by C. songaricum by characterizing and comparing the rhizosphere volatile metabolomes of five host plant species. Gas chromatography–mass spectrometry (GC–MS) was used to analyze the rhizosphere volatiles of three Nitraria species (N. roborowskii, N. sibirica, N. tangutorum), Peganum multisectum, and Zygophyllum xanthoxylum. Multivariate statistical analyses, including PCA, HCA, and OPLS-DA, were employed to identify shared and differential metabolites. A total of 116 volatile compounds were identified. Alkanes were the predominant metabolite class (accounting for 46.27–76.47% in each host), and 11 C₁₁–C₁₆ alkanes were shared by all species. Notably, Z. xanthoxylon (belonging to a different family) exhibited a distinct metabolic profile, with a significantly higher proportion of benzene derivatives (35.82%) compared to the other hosts. PCA and cluster analysis revealed family/genus-specific clustering patterns, with Z. xanthoxylon forming a separate cluster. Several differential metabolites unique to Z. xanthoxylon possessed antimicrobial and stress-resistant activities. This study reveals the chemical signatures of C. songaricum host roots. The shared alkanes are proposed as potential background signals for general recognition, though this hypothetical role requires experimental validation. Family/genus-specific compounds might be involved in host selection. The unique chemical profile of Z. xanthoxylon suggests that C. songaricum may employ a flexible recognition strategy, enabling it to parasitize plants across different families. These findings provide fundamental data for understanding the chemical basis of host–parasite interactions in this species. Full article

Industrial PLC programming faces persistent difficulties: lengthy development cycles, low fault tolerance, and cross-platform incompatibility among vendors. While LLMs show promise for automated code generation, their direct application is hindered by the gap between ambiguous natural language and the strict determinism required by control logic. This paper proposes MPC-Coder, a dual-knowledge enhanced multi-agent system that addresses this gap. The system combines a structured knowledge graph that imposes hard constraints on process parameters and equipment specifications with a vector database that offers implementation references such as code templates and function blocks. These two knowledge sources form a symmetric complementary architecture. A closed-loop “generation–verification–repair” mechanism leverages formal verification tools to iteratively refine the generated code. Experiments demonstrate that MPC-Coder achieves 100% syntactic correctness and 78% functional consistency, significantly outperforming general-purpose LLMs. The results indicate that the complementary fusion of domain knowledge and closed-loop verification effectively enhances the reliability of code generation, offering a viable technical pathway for the reliable application of LLMs in industrial control systems. Full article

Myopathies represent a highly heterogeneous group of primary muscle disorders, traditionally classified based on clinical presentation and histopathological findings. Recent breakthroughs in molecular genetics, immunology, and pathophysiology have revolutionized the understanding, diagnosis, and management of these conditions. Both inherited and acquired forms of myopathy, including structural, metabolic, inflammatory, endocrine, and mitochondrial subtypes, are now recognized to arise from diverse pathogenic mechanisms such as impaired calcium handling, mitochondrial dysfunction, chronic inflammation, altered metabolism, and defective muscle regeneration. The advent of next-generation sequencing technologies has enabled more precise diagnosis of genetic forms, while the discovery of novel molecular biomarkers and immunological signatures offers promising avenues for disease monitoring and stratification across the broader spectrum. Importantly, molecular and mechanistic insights have redefined clinical classifications, allowing for better prognostic predictions and patient-tailored therapeutic approaches. Innovative treatments, including gene therapy, antisense oligonucleotide therapies, immune-modulating agents, metabolic support strategies, and targeted pharmacological interventions, are progressively translating molecular knowledge into clinical applications. However, technical limitations, biological variability, and ethical considerations continue to pose significant challenges to the implementation of precision medicine in myopathies. In this narrative review, we comprehensively discuss the latest molecular findings, their integration into clinical practice, and the emerging therapeutic strategies based on these discoveries. We also highlight current limitations and propose future research directions aimed at bridging the gap between molecular insights and effective, equitable patient care. Full article