Search Results (9,616)

Honey is a high-value food product that is vulnerable to adulteration with exogenous sugars, posing challenges for food authenticity and consumer protection. This study applied Stable Isotope Ratio Analysis (SIRA) to assess the authenticity of honey collected from three major honey-producing regions of Ghana (Volta, Bono and Bono East). A total of 28 honey samples were analysed by elemental analysis–isotope ratio mass spectrometry (EA-IRMS) to obtain carbon (δ¹³C), nitrogen (δ¹⁵N) and sulphur (δ³⁴S) isotope composition. Honey authenticity was evaluated according to AOAC Official Method 998.12 by comparing δ¹³C values of bulk honey and the corresponding protein fraction. The δ¹⁵N and δ³⁴S values in honey protein were used to investigate environmental and regional variability. Samples without detectable C4 adulteration exhibited δ¹³C_protein values consistent with C3 floral sources, whereas several samples showed Δδ¹³C values more negative than −1.0‰, indicating the presence of C4-derived sugars above the AOAC adulteration threshold. Calculated C4 sugar contents ranged from 8 to 12% in moderately adulterated samples to as high as 78–79% in severely adulterated samples, confirming substantial dilution with C4 sugars. Nitrogen and sulphur isotope ratios provide additional information on environmental and regional variability among the sampled regions. Principal Component Analysis revealed that the first two principal components (PC1 and PC2) accounted for 83.8% of the total variance 83.8% of the total variance and showed separation between samples with detectable C4 adulteration and those without, while highlighting regional isotopic differences. These results demonstrate that stable isotope analysis is an effective tool for detecting C4 sugar adulteration in honey and that the combined use of carbon, nitrogen and sulphur isotopes can provide additional information on environmental and regional variability. These findings provide preliminary isotopic data on honey collected from three major honey-producing regions of Ghana and support the application of the stable isotope approach for honey authenticity assessment and quality control. Full article

Accurate maize distribution information is critical for crop-area statistics, food-security assessment, and agricultural monitoring, but large-scale maize-mapping remains difficult in regions with limited reference samples, heterogeneous crop calendars, and frequent optical data gaps. This study proposes a phenology-adaptive maize mapping framework based on Sentinel-2 time-series imagery and an Enhanced Red-edge NDVI (ENDVIre). ENDVIre was constructed from the Sentinel-2 red-edge 4 and red-edge 2 bands to enhance the spectral response of maize during the silking-to-grain-filling stage, when maize develops a dense canopy and high chlorophyll content but is often confused with soybean. The framework first reconstructed the NDVI time series using an upper-envelope-constrained Whittaker smoother to identify key phenological stages, including sowing–emergence, vigorous growth, and maturity–harvest. NDVI, ENDVIre, and LSWI were then integrated into an interpretable decision-tree model with phenology-aligned time windows to distinguish maize from soybean, rice, wheat, and other non-maize backgrounds. The method was evaluated in six representative maize-growing regions across the United States, Brazil, China, Kenya, and Ukraine, covering different crop calendars, field sizes, and agricultural systems. The mean overall accuracy, F1-score, and Kappa coefficient across the six regions reached 93.27%, 93.14%, and 0.8652, respectively. Cross-year experiments in a winter-wheat–summer-maize rotation region from 2020 to 2024 achieved overall accuracies of 89.80–96.80%, while spatial-transfer experiments in six independent regions achieved overall accuracies of 87.40–95.40%. A comparison with existing high-resolution maize products in the Huang-Huai-Hai Plain further showed that the proposed method better balanced omission and commission errors. These results indicate that ENDVIre-based phenology rules provide an interpretable and transferable solution for maize mapping under limited-sample conditions, although persistent cloud contamination and fragmented smallholder landscapes remain important challenges. Full article

Food delivery riders face a structural shortage of informal street rest spaces in urban public environments, yet existing facilities often fail to match their highly mobile labor processes. Taking the Hexi University Town commercial district in Changsha as a case study, this research examines how rest-space conditions are associated with riders’ occupational dignity and work environment satisfaction. Based on 365 valid questionnaires, field observations, and informal interviews, structural equation modeling, bootstrap mediation analysis, and grouped regression analysis were conducted within a spatial justice framework. The results show that spatial justice perceptions are associated with satisfaction through differentiated pathways. Spatial embeddedness is associated with work environment satisfaction, while facility suitability operates partly through occupational dignity and has the highest mediation proportion. Procedural justice is insignificant in formal spaces but has a strong effect in informal spaces, revealing a mismatch between institutional provision and practical accessibility. The findings indicate that riders’ rest-space dilemma stems not only from insufficient facilities but also from the disembedding of spatial rights from mobile labor processes. This study extends spatial justice research from resource distribution to labor-process embeddedness and proposes micro-renewal strategies that shift from facility provision to process embeddedness, offering implications for inclusive public-space planning, sustainable urban design, and urban governance. Full article

Chemical contamination of milk represents a relevant food safety concern, particularly in countries where systematic monitoring programs are still limited. In Algeria, information on human exposure to aflatoxin M₁ (AFM₁) and per- and polyfluoroalkyl substance (PFAS) contamination remains fragmented or lacking. This study assessed the occurrence of AFM₁ and 17 PFAS in cow and goat milk and explored farm-level management practices potentially influencing contamination. Bulk-tank milk samples were collected from 26 dairy cattle and 11 dairy goat farms. Farm characteristics, including feeding strategies, water sources, grazing practices, and herd size, were recorded using structured questionnaires to characterize farm-level practices and explore their potential association with contamination patterns. AFM₁ was detected in all samples. In cow milk, 96.1% exceeded the EU limit (0.05 µg/kg), with concentrations ranging from 0.048 to 0.410 µg/kg (mean: 0.102 µg/kg), although none exceeded the Codex Alimentarius limit (0.50 µg/kg). Goat milk showed a lower prevalence of samples above the EU limit (36.4%), but higher concentrations were observed (range 0.030–0.578 µg/kg; mean 0.193 µg/kg), with two samples (18.2%) exceeding the Codex limit. PFAS contamination was generally low: 73.0% of cow and 64.0% of goat samples were below detection limits, with only PFBS (cow milk) and PFDA (goat milk) quantified above LOQ. Because of the limited number of farms and the single sampling period, no robust associations could be established between farm-level variables and contaminant concentrations. This preliminary study provides an exploratory overview of AFM₁ and PFAS occurrence in bovine and goat bulk milk from the sampled Algerian farms. These findings reveal widespread AFM₁ contamination within the investigated farms, consistent with possible feed-borne AFB₁ exposure, whereas PFAS occurrence appeared sporadic and generally low. Full article

Sustainable agricultural development in natural heritage sites poses a challenge, requiring food security without compromising the conservation of ecosystems and their outstanding universal values (OUV). The Galapagos Islands, recognized as a Natural World Heritage, have problems of scarce water and arable land, compounded by anthropogenic pressures such as high population and tourism growth and dependence on food imports. The objective of this research is to evaluate the environmental impacts of implementing agricultural greenhouses in the Galapagos by applying a traditional environmental matrix alongside a UNESCO World Heritage approach, integrated with a Strengths, Weaknesses, Opportunities, and Threats (SWOT) analysis, to formulate strategies for strengthening local agriculture without compromising ecosystems. This study employed a semi-quantitative methodological approach, integrating three key aspects: (i) a baseline of agricultural information and water availability on the islands; (ii) an integrated Environmental Impact Assessment (EIA) approach to greenhouse implementation; and (iii) sustainable agricultural development and environmental impact mitigation strategies. The results of the traditional EIA and the UNESCO approach through the OUV showed negative impacts classified as insignificant to moderately significant. For the evaluated design, these impacts can be managed through the active participation of academia, the community, and government entities. However, their scalability depends on a more in-depth analysis of the potential long-term risks associated with the availability of natural resources, microplastic pollution, and the use of agrochemicals. Among the proposed strategies, the importance of monitoring water and soil quality and of agricultural and environmental education campaigns in the community was highlighted. This study presents agricultural greenhouses as well-known alternatives for food self-sufficiency, adapted to the realities of the island territory and the objectives of ecosystem conservation. The proposed methodological approach can be applied in protected areas to promote conservation and sustainable agricultural production. Full article

Agritourism farm-stay guesthouses represent a burgeoning sector of rural tourism, wherein locally produced food serves as the primary experiential attraction. This study examines tourist perceptions regarding food quality, sensory characteristics, sustainability awareness, loyalty indicators, and comparative evaluations at three farm-stay guesthouses in Caraș-Severin County, Romania, located at distinct altitudes: lowland (Sacu, 154 m a.s.l.), hill (Văliug, 550 m a.s.l.), and mountain (Cozia, 1130 m a.s.l.). Altitude in this study marks three distinct settings—lowland, hill, mountain—rather than functioning as a tested independent variable. The results show that tourists evaluated all three guesthouses similarly, with no statistically significant differences across zones. The comparative design was a way of asking whether own-farm food quality perceptions hold across different agritourism contexts, not a test of what altitude does to those perceptions. A structured questionnaire (n = 650) was distributed to guests following an informed consent protocol. Four latent constructs were operationalised: food quality (FQ; Cronbach’s α = 0.593), sensory characteristics (SCs; α = 0.596), sustainability perception (SP; α = 0.393), and comparison with non-farm establishments (CF; α = 0.621). Overall gastronomic satisfaction was particularly high (mean = 4.71 ± 0.62 on a 1–5 Likert scale), and the average overall score was 9.44 ± 1.01 out of 10. Multiple regression accounted for 7.5% of the satisfaction variance (R² = 0.075; F(4,643) = 13.09, p < 0.001), with sensory characteristics (β = 0.232, p < 0.001) and sustainability perception (β = 0.088, p = 0.020) serving as significant predictors. Food origin transparency substantially impacted satisfaction (ANOVA: F(3,646) = 4.964, p = 0.002): visitors who received thorough provenance explanations were more satisfied (mean = 4.77) than those who received no information (mean = 4.57). Among the 569 respondents with prior non-farm experience, 85.2% rated farm-stay cuisine as superior to non-farm alternatives overall. Food quality perceptions in these three Caraș-Severin guesthouses are uniformly high regardless of altitude. What separates more satisfied guests from less satisfied ones is not the measurable quality of the product but whether the host explained where it came from. Full article

Alzheimer’s disease (AD) is a multifactorial neurodegenerative disorder characterized by impaired glucose metabolism, mitochondrial dysfunction, inflammation, oxidative stress, and progressive cognitive decline. Because currently available pharmacological therapies provide only modest symptomatic benefit, nutrition-based interventions are increasingly being explored as complementary strategies for supporting brain metabolism and cognitive resilience. The KetoFLEX 12/3 dietary pattern, developed within the ReCODE (Reversal of Cognitive Decline) program, is a plant-rich, mildly ketogenic nutrition and lifestyle framework that integrates low-glycemic nutrition, time-restricted eating, and personalized metabolic optimization. The diet emphasizes deeply pigmented non-starchy vegetables, extra-virgin olive oil, nuts and seeds, omega-3-rich seafood, and minimally processed foods while limiting refined carbohydrates, sugars, processed foods, and selected grains and dairy products. Emerging mechanistic and clinical evidence suggests that KetoFLEX 12/3 may influence several pathways relevant to AD pathophysiology, including insulin signaling, mitochondrial bioenergetics, neuroinflammation, oxidative stress, autophagy, detoxification pathways, and gut–brain axis function. Observational findings from ReCODE-related studies have reported improvements in metabolic parameters, mood-related outcomes, cognitive measures, and brain volumetrics in participants adhering to multimodal precision-medicine interventions incorporating the KetoFLEX principles. Compared with traditional dietary models such as the Mediterranean or MIND diets, KetoFLEX 12/3 places greater emphasis on mild nutritional ketosis, meal timing, and metabolic personalization based on factors such as ApoE genotype and insulin sensitivity. The objective of this Perspective is to examine the mechanistic rationale, emerging evidence, limitations, and future research priorities for KetoFLEX 12/3 as a precision-nutrition framework for cognitive health in AD. Although much of the current evidence remains mechanistic, observational, or derived from multimodal intervention studies, the framework offers a biologically plausible precision-nutrition model that may inform future research and clinical investigation in cognitive decline. Full article

Winter cereals, which are vital for global food security in temperate regions, face severe challenges during overwintering due to the development of snow mold—a complex disease caused by different microorganisms that combine phytopathogenicity with cold tolerance. Even within a single field plot, individual plants exhibit significant variation in snow mold severity. This natural variation was exploited to achieve the aim of the present study—the comparison of microbiomes of healthy and diseased plants of winter cereal crops (rye, triticale, and wheat) at the peak of snow mold manifestation to interpret differential disease severity through differences in plant-associated microbial communities and to obtain information necessary for the biological control of snow mold. Fungi of the genus Herpotrichia were implicated as novel candidate causal agents of snow mold in winter cereals. Variations in snow mold severity defy simple explanations tied solely to pathogen abundance or broad changes in overall microbial community composition. Instead, the most striking contrast between healthy and diseased plants was observed in the inferred candidate hub taxa, accompanied by marked changes in exploratory co-occurrence networks involving the candidate snow mold pathogens. These network alterations were crop-specific. Several key taxa were implicated as probable influencers of snow mold dynamics. Full article

The safety of non-food products is embedded in the United Nations 2030 Agenda for Sustainable Development and the European Union (EU) framework, supporting health protection, responsible production and consumption, and market surveillance. The EU Rapid Alert System for dangerous non-food products, known as Safety Gate (formerly RAPEX), was established in 2005 to facilitate the exchange of information on products posing risks within the internal market. The aim of this study was to present the interdependencies reported in the Safety Gate system/RAPEX in 2005–2025, considering: product category, type of risk, country of origin, notifying country and year, as well as measures taken. The VOSviewer 1.6.20 and Statistica 13.3 were used. The results highlighted the following problems: toys from China with chemical, choking and injury risks; electrical appliances also from China with electric shock hazards; motor vehicles from Germany with injury risks; cosmetics from Italy with chemical and microbiological risks; and clothing from Turkey with suffocation risks. Reporting is expected to continue under existing regulatory frameworks, although changing the name of the system from RAPEX to “Safety Gate” may reduce its recognition. The findings highlight the need for targeted enforcement, improved risk profiling by product category and origin, and ongoing monitoring of emerging safety risks. Full article

This paper explores the culinary and cultural significance of cooked leafy vegetables among Afro-descendant communities in French Guiana and Suriname, including French Guianese and Surinamese Creoles, Maroons, and Haitian migrants. While leafy greens play a major dietary role across sub-Saharan Africa, their consumption in the Americas remains understudied. This ethnobotanical study of edible leafy plants is based on surveys of local markets, gardens and residents. Drawing on 26 informal interviews conducted in four local languages (French, French Guianese Creole, Haitian Creole, and Nengee Tongo), we describe 36 species of edible leaves from 20 plant families consumed in the region. Our findings show that although the practice of eating leafy greens is widely shared, the species selected, their names, and their perceived properties vary noticeably across cultural groups. Some plants are eaten exclusively by Maroons (e.g., Cestrum latifolium, Capsicum spp.), others by Haitians (e.g., Corchorus olitorius, Rivina humilis), and some have fallen into disuse among younger generations. These differences are shaped by ecological availability, cultural memory, food-medicine beliefs, and interethnic influences. We suggest that the term callaloo (referring to both dishes and leafy vegetables), which circulates in multiple linguistic and culinary forms throughout the African diaspora, can serve as a metaphor for the interculturalization of foodways. More than ingredients, these leafy vegetables act as dynamic cultural markers—symbols of resilience, transmission, and transformation. In a context of rapid globalization, where unseen foods risk sinking further into obscurity, these plant-based traditions highlight both the adaptability and fragility of Afro-descendant culinary heritage in the Guiana Shield. Full article

Although chitosan (CS)- and essential oil (EO)-based packaging systems have been widely reviewed, a focused synthesis connecting nano-delivery design with interfacial regulation, film-network evolution, release behavior, and preservation performance in real food systems remains lacking. This review addresses that gap by examining CS-based nano-delivery systems for EOs in active food packaging, with an emphasis on how carrier design and multiscale organization govern functional performance. Major delivery strategies, including nanoemulsions, nanoparticles, nanogels, Pickering emulsions, nanofibrous systems, and nanocomposites, are discussed in relation to EO stabilization, dispersion uniformity, and controlled release. Their effects on film microstructure, mechanical and barrier properties, thermal stability, optical behavior, and antimicrobial and antioxidant activities are further evaluated alongside preservation outcomes in fruits, vegetables, dairy products, meat, and aquatic products. Particular attention is given to structure-function relationships across the carrier, interface, and film-network levels, and to the distinction between established active-packaging functions and emerging smart-packaging applications. Current challenges include EO compositional variability, limited cross-study comparability, sensory constraints, migration and regulatory concerns, and insufficiently scalable fabrication routes. Future work should prioritize mechanism-informed interfacial design, standardized evaluation frameworks, food-specific release-preservation correlations, and scalable green manufacturing. Full article

Background: The transition to motherhood represents a critical life phase marked by profound biological, psychological and social changes. During this period, women’s nutritional practices are shaped not only by physiological needs but also by shifting identities, caregiving responsibilities and social expectations. Although nutrition during pregnancy and the postpartum period has been widely studied from a biomedical perspective, less attention has been paid to how women experience, negotiate and attribute meaning to food during the transition to motherhood. Objective: This systematic qualitative review aimed to synthesise existing qualitative evidence on women’s experiences of nutritional practices during the transition to motherhood, with particular attention to food as self-care, control, autonomy, identity formation and mental well-being. Methods: A systematic search of electronic databases was conducted to identify qualitative studies exploring women’s experiences of nutrition during pregnancy and early motherhood. Eligible studies employed qualitative methodologies such as interviews, focus groups or ethnographic approaches. Study selection followed PRISMA guidelines. Methodological quality was appraised using established qualitative appraisal tools. A thematic synthesis approach was used to integrate findings across studies. Results: The synthesis identified several interrelated themes: nutrition as a form of self-care and emotional regulation; loss of autonomy and heightened moral surveillance around food choices; food practices as a means of performing and negotiating “good motherhood”; and the emotional burden of dietary expectations in relation to mental health and identity. Women described navigating competing demands between their own nutritional needs and those of their infants, often within contexts of social judgement and limited support. Conclusions: Nutritional practices during the transition to motherhood extend beyond health behaviours and are deeply embedded in issues of identity, autonomy and care. Recognising the social and emotional dimensions of maternal nutrition may inform more holistic, woman-centred approaches to nutritional guidance and maternal health support. Full article

Background: To investigate the diagnostic value of the SPT-to-histamine ratio, casein-specific immunoglobulin E (IgE), and cow’s milk-specific IgE in predicting oral food challenge (OFC) positivity in children with suspected cow’s milk allergy. Methods: In this single-center retrospective observational study, 126 children evaluated for suspected cow’s milk allergy who underwent OFC between January 2019 and July 2024 at a tertiary pediatric allergy and immunology center were included. Demographic and clinical characteristics, SPT parameters, and laboratory biomarkers were analyzed. Discriminatory performance was assessed using receiver operating characteristic (ROC) analysis, and independent predictors were identified using multivariable logistic regression. Results: OFC was positive in 66 patients (52.4%). Casein-specific IgE demonstrated strong discriminatory performance (AUC = 0.878), with 80.3% sensitivity and 85.0% specificity at a cutoff value of ≥2.55 kUA/L. The SPT-to-histamine ratio showed moderate discriminatory performance (AUC = 0.758). In multivariable analysis, only casein-specific IgE remained an independent predictor of OFC positivity (adjusted OR = 1.317, p < 0.001). Conclusions: Casein-specific IgE is a strong independent predictor of OFC positivity. Although SPT-derived ratios may provide complementary diagnostic information, their independent predictive contribution appears limited. These findings support the role of component-resolved diagnostics in pre-challenge risk stratification. Full article

Accurate rice yield estimation is essential for food security. Two key factors affecting estimation accuracy are the long-term upward trend in yield over time and regional heterogeneity across space. Current studies predominantly employ statistical detrending methods (e.g., moving averages, linear regression) to isolate temporal trends. However, such methods rely on prior assumptions about the time–yield relationship and may introduce systematic bias when these assumptions break down. Meanwhile, the individual contributions of temporal and spatial information, and their interactive effects, have not been systematically evaluated within a unified framework. We selected 112 rice-growing counties across six U.S. states (2000–2021), using vegetation index (Normalized Difference Vegetation Index), meteorological indicators (growing degree days, killing degree days, and cumulative precipitation), and spatiotemporal variables (year, longitude, and latitude). We designed six input configurations to compare conventional detrending against direct temporal variable inclusion, testing across four model architectures (Long Short-Term Memory, Random Forest, XGBoost, and Transformer). Results showed that: (1) directly inputting year significantly outperformed detrending across all models, with the combined spatiotemporal configuration achieving the best performance (LSTM R² = 0.61 vs. 0.54 for detrending); (2) year was the most important predictor in SHAP analysis, with spatiotemporal variables ranking higher than most meteorological and remote sensing variables; (3) spatial information consistently improved accuracy and mitigated systematic bias for extreme yield regions; (4) the combined configuration performed best across different states, years (including extreme climate events), and yield levels, achieving near-end-of-season accuracy at the grain-filling stage (1.5–2 months before harvest). This study demonstrates that integrating raw spatiotemporal data directly into deep learning models is more effective than statistical detrending, offering a simpler and more robust approach for large-scale crop yield estimation. Full article

The discoveries of most ancient wooden objects (MAWOs) (defined here as older than 200,000 years) have shed light on human evolution, particularly the cognitive ability and advanced woodworking skills of early hominins that enabled them to meticulously construct wooden objects for shelter and wooden tools for specific applications, such as hunting and digging for underground plant products for food. These discoveries give us insight into the complex behaviour and technological development of early human ancestors, who migrated to various parts of the world, adapting to local environments and taking advantage of available resources for food and shelter. This review focuses on five MAWOs unearthed in different parts of the world and dating from 300,000 to 780,000 years ago, representing examples of early hominins’ use of wood to ingeniously construct objects for specific purposes. Background information on wood composition and structure, and the diagnostic features of wood cell wall degradation by erosion bacteria is included, as these bacteria are the main microorganisms that degrade the cell wall of ancient wooden objects buried in waterlogged, anoxic environments, such as the 300,000-year-old Schöningen spears, which, although remarkably well preserved, had been surface-degraded by erosion bacteria. Full article