Search Results (27)

Chilacayote (Cucurbita ficifolia Bouché) is recognized as a rich source of nutrients and bioactive compounds, making it a promising ingredient for fortifying staple foods such as corn tortillas. While fortification can improve nutritional properties, it may also alter sensory characteristics that determine consumer acceptance. Therefore, a rigorous and structurally grounded assessment of these sensory modifications is required. In this study, sensory evaluations were conducted with regular tortilla consumers using Check-All-That-Apply (CATA) questionnaires to examine six attributes (color, smell, texture, taste, mouthfeel, and aftertaste) in tortillas made with nixtamalized dough and commercial flour, both with and without chilacayote powder. Then, a structured framework for dimensionality reduction and sensory profile identification of tortillas is proposed. In this framework, three classical feature extraction methods (Principal Component Analysis (PCA), Linear Discriminant Analysis (LDA), and a combination of both (PCA+LDA)) were compared with an evolutionary discriminant approach (Differential Evolutionary Linear Discriminant Analysis for Feature Extraction and Visualization (DE-${\mathrm{LDA}}_{FE}$)). The projection quality of these methods was evaluated using a multi-scale separability index that integrates global, semi-global, and local metrics, and the experiments were conducted considering global and attribute-based analyses. Beyond quantitative discrimination, the optimized projections enabled a geometric interpretation that allows the identification of sensory profiles for the tortilla variants. The proposed methodology bridges evolutionary optimization, structural separability assessment, and interpretable sensory characterization, offering a robust and adaptable strategy for multivariate food analysis and other complex discrimination problems and insights into the sensory impact of chilacayote fortification for the development of nutritionally enhanced tortillas that preserve consumer appeal. Full article

Background/Objectives: Metabolic diseases are increasingly associated with diets low in bioactive compounds. Native maize varieties possess functional potential; however, they remain underutilized. Moringa oleifera leaf flour (MF), rich in protein and polyphenols, represents a promising functional ingredient. This study evaluated the incorporation of MF into red native corn tortillas and its effects on nutritional composition and antioxidant capacity, as well as assessed its hypoglycemic and hypolipidemic effects in Wistar rats. Methods: Tortillas were formulated with 5% MF. Nutritional composition was determined using standard AOAC methods, while bioactive compounds (total phenolics and flavonoids) and antioxidant activity were evaluated using Folin–Ciocalteu, aluminum chloride (AlCl₃) colorimetric, DPPH^•, and ABTS^•+ assays, respectively. Male Wistar rats (12 weeks old, with an approximate weight ofs 360 g; n = 5/group) were fed the experimental diets for 21 days with either a standard diet, a high-fat diet, or high-fat diets supplemented with MF or MF-enriched tortillas. Serum glucose, triglycerides, total cholesterol, and HDL were measured using enzymatic colorimetric methods. Data were analyzed by ANOVA followed by Tukey’s test (p < 0.05). Results: MF incorporation increased protein (+19.85%), dietary fiber (+18.51%), and mineral content (+41.03%) compared to control tortillas. Total phenolics and flavonoids increased by 114.0% and 184.7%, respectively. Antioxidant activity improved significantly, as evidenced by reductions in IC₅₀ values of 41.1% (DPPH^•) and 43.1% (ABTS^•). In vivo, MF-enriched tortillas reduced triglycerides by 68.4%, total cholesterol by 16.2%, and hepatic lipid accumulation by 31.8% compared to the high-fat diet group. Glucose levels showed a reduction of 8.5%, although not statistically significant (p > 0.05). Conclusions: The incorporation of MF into red corn tortillas significantly enhances their nutritional and functional properties. In vivo results also showed improvements in lipid profile and a non-significant reduction in glucose levels. These findings support the development of functional foods based on traditional staples with potential health benefits. Full article

Background/Objectives: Soy flour has been proposed as a functional ingredient to improve the protein and fiber content of foods; however, its metabolic and sensory effects, particularly in individuals at elevated risk for metabolic disease, remain insufficiently characterized. This randomized, repeated-measures study examined whether substituting refined wheat or corn flour with soy flour influences postprandial glucose and insulin plasma concentrations, appetite ratings, and product acceptability in adults with overweight or obesity. Methods: Participants (N = 17) attended at least three separate visits during which they consumed, in random order, a food matrix with 0% (control), 10%, or 30% soy flour substitution. Food matrices included breads (n = 10), tortillas (n = 10), and arepas (n = 8); some participants completed more than one matrix. Postprandial plasma glucose and insulin concentrations were measured at baseline and at 15, 30, 60, 90, and 120 min post-ingestion. Subjective hunger, satiety, and product liking were assessed using a 10 cm visual analog scale. Results: Compared with the control condition, substituting 30% of refined flour with soy flour significantly reduced the area under the concentration–time curve for postprandial glucose for breads (p = 0.03) and arepas (p = 0.04), and reduced plasma glucose concentrations at 90–120 min for tortillas (p = 0.0009). In contrast, postprandial insulin concentrations and subjective hunger and satiety ratings did not differ across substitution levels or food matrices (all p > 0.05). Importantly, even 30% soy flour substitution maintained product liking. Conclusions: Incorporating up to 30% soy flour may improve postprandial glycemic responses without compromising overall liking, supporting its potential as a practical food reformulation strategy to improve metabolic health in populations at increased risk of metabolic disease. Full article

Tortillas are an essential food staple in the Mexican diet due to their nutritional value. Blue corn tortillas have been reported as a source of bioactive compounds, such as phenolic compounds and flavonoids. Likewise, the blue corn tortillas have been studied to enhance the nutritional and nutraceutical composition. In this sense, Quelites are a large family of plants with macronutrient and micronutrient content, as well as a source of phenolic compounds, flavonoids, and carotenoids. Among these, Amaranthus hybridus L. could fortify the blue corn tortilla composition. Therefore, this study aims to fortify blue corn tortillas with different concentrations of Quelites flours. The total flavonoid and phenolic compounds content, as well as the antioxidant capacity and identification of phenolic compounds, were evaluated on tortillas fortified with Quelites. The addition of Quelites to blue corn tortillas reduced the lipid and protein content, carbohydrate, and flavonoid content, and enhance the antioxidant capacity of tortillas as measured by FRAP, ORAC, and TEAC assays. Also, caffeic acid, chlorogenic acid, ferulic acid, and sinapic acid were identified on blue corn tortillas fortified with Quelites. These results support the use of A. hybridus L. as an ingredient to improve the nutrient and nutraceutical composition of foods. Full article

Edible insects, despite their high nutritional value, are widely rejected due to neophobia associated with eating them whole. This study evaluated crispy corn tortillas (tostadas) enriched with cricket flour (CF) and defatted cricket flour (DCF) to increase protein content and acceptability. The tostadas were prepared using CF and DCF as substitutes for corn flour (5–25%). Physicochemical evaluation was conducted using proximal analysis, color measurements, and pH measurements. Sensory evaluation included Check all that apply (CATA); general liking, Just about right (JAR); preference mapping; and penalty analysis. The results showed increases in protein, fat, and ash content, and decreases in moisture and carbohydrates. The samples with cricket flour exhibited b* values similar to the control, an increase in a*, and a decrease in L*. The most notable descriptors for the tostadas with cricket flour were herb flavor, brown color, and granular texture. The treatments containing 5% and 15% CF showed the highest liking scores, whereas the control sample presented the lowest level of acceptance. In this regard, the control sample reduced the overall mean liking score. Therefore, most consumers exhibited preference patterns toward the samples formulated with CF and DCF, which were associated with higher overall acceptability. The penalty analysis indicated that the samples should be saltier and darker. In conclusion, tortillas enriched with cricket flour increase protein content and acceptability, although the formulation could be further improved to enhance overall liking. Full article

In recent years, there has been a growing interest in developing sustainable food products that consider not only economic but also environmental and social impacts. Therefore, this study aimed to prepare fortified tortillas with ayocote (Phaseolus coccineus) and quintonil (Amaranthus hybridus) flours, analyzing the nutritional, functional, physical (color, rollability) and textural properties. Different concentration levels (3, 6, and 9%) and flour mixtures (1:1, 2:1, and 1:2) of quintonil/ayocote were evaluated. The most remarkable chemical composition changes were observed for a 9% substitution, where the contents of protein, ash, and fiber were 1.29, 1.79, and 2.73 times greater than those of the control sample, respectively. In the fortified tortillas, both total phenolic content and antioxidant activity decreased. This can be attributed to the distinct chemical interactions present in the prepared mixtures, which restrict the release of bioactive compounds. Changes were also observed in textural and color properties; however, rollability was not compromised. It is worth noting the potential of this innovation, which can contribute to enhancing nutrition and ensuring food safety, promoting the consumption and preservation of the underutilized plant species employed. Full article

A competitive electrochemical immunosensor, using screen-printed carbon electrodes (SPCEs), was developed for the determination of total fumonisins (sum of FB1, FB2 and FB3) extracted with a simple solvent extraction and dilution method, without clean up, from maize flour and maize tortillas. The optimized biosensor has a linear range of 0.25 to 50 µg/L with 3% and 2% reproducibility for FB1 and (FB1 + FB2), respectively, and a linear range of 0.25 to 10 µg/L with 2% reproducibility for (FB1 + FB2 + FB3). The limits of detection and quantification in PBS buffer for total fumonisins are 0.12 µg/L and 0.39 µg/L, respectively. These values in the maize matrix are 6.07 µg/kg and 20.25 µg/kg, respectively. In addition, the stability and the selectivity of the sensor were studied. The immunosensor was validated with liquid chromatography–tandem mass spectrometry. This novel biosensor is more rapid, simpler and cheaper than current methods, and can also be used at the point of need. Full article

Traditional taco preparation methods, such as oil immersion and steaming, can significantly affect the nutritional and metabolic characteristics of the final product. This study evaluated tacos made with five commercial nixtamalized maize flours and four common fillings (chicharron, beef skirt, potato, and refried beans), processed using three different methods: Plain, Full-Fat, and Patted-Dry. We assessed their chemical composition, starch digestibility, and thermal properties, and measured satiety-related hormone responses in mice. Fillings had a stronger influence on protein, fat, and moisture content than tortilla type. Full-fat tacos exhibited increased amylose–lipid complex formation and a lower gelatinization enthalpy, whereas plain tacos retained more retrograded starch and a crystalline structure. In vitro digestion revealed that Plain tacos, especially those with plant-based fillings, had the highest resistant starch content and the lowest predicted glycemic index. Hierarchical clustering showed that resistant starch, moisture, and gelatinization onset temperature were closely linked in the Plain samples, whereas lipid-driven variables dominated in the Full-Fat tacos. In mice, tacos with a higher resistant starch content led to greater GLP-1 levels, lower ghrelin levels, and reduced insulin responses, suggesting improved satiety and glycemic control. Patted-Dry tacos showed intermediate hormonal effects, supporting their potential as a balanced, health-conscious alternative. These findings demonstrate how traditional preparation techniques can be leveraged to enhance the nutritional profile of culturally relevant foods, such as tacos. Full article

Background/Objectives: The problems with following a gluten-free (GF) diet result from the high cost of GF products, their limited availability for celiac disease (CD) patients, and their disputable quality. The aim of this cross-sectional study was to assess the frequency of buying and availability of GF cereal products in a population of Polish female CD patients. Methods: This study was conducted in a population of Polish female CD patients who were members of the Polish Celiac Society, and n = 547 respondents were included in this study. Participants were asked about the frequency of buying and problems with the availability of GF cereal products, which were compared by sub-groups stratified by age, place of residence, place of purchasing major grocery shopping and purchasing GF products online. Results: The majority of the studied female CD patients declared often purchasing GF flour, pasta, and bread, as well as never purchasing GF puff pastry, fried baked goods, dumplings, and crackers. The only product for which the majority of the studied participants declared problems with availability was dumplings. For younger respondents, a higher share declared often buying GF pasta (p = 0.0073), chips, crisps and puffs (p < 0.0001), and Asian-style noodles (p = 0.0269), as well as declared problems with the availability of GF wraps/tortillas (p = 0.0001), puff pastry (p = 0.0294), fried baked goods (p = 0.0008), biscuits/cookies (p = 0.0148), and Asian-style noodles (p = 0.0046) compared to older respondents, while for older respondents, a higher share declared often buying GF flour (p = 0.0358), and never buying GF wraps/tortillas (p = 0.0181). For respondents living in big cities, a higher share declared problems with the availability of GF pasta compared to respondents living in small towns/villages (p = 0.0245). For respondents purchasing major grocery shopping in hypermarkets, a higher share declared often buying GF biscuits/cookies compared to respondents purchasing in other shops (p = 0.0039), while for respondents purchasing in other shops, a higher share declared never buying puff pastry (p = 0.0076), dumplings (p = 0.0002), and wraps/tortillas (p = 0.0038), as well as declared problems with availability of GF puff pastry (p = 0.0246), biscuits/cookies (p = 0.0002), and breakfast cereals (p = 0.0011). For respondents not purchasing GF products online, a higher share declared never buying GF fried baked goods compared to respondents purchasing online at least occasionally (p = 0.0284), as well as a lower share declared problems with the availability of GF wraps/tortillas (45% vs. 33%, p = 0.0411). Conclusions: The population of Polish female CD patients seems quite diverse in terms of the chosen GF cereal products, with age, primary place of purchasing major grocery shopping and purchasing GF products online, but not the place of residence, as the major determinants. The declared problems with the availability of GF products are probably associated with two diverse mechanisms—either frequent purchasing (as individuals not purchasing may not be interested in such a product at all) or rare purchasing (which may result from poor availability). Increasing the availability of GF cereal products for a population of Polish female CD patients may allow them to obtain a more diverse diet. Full article

This study evaluated the nutritional composition, techno-functional properties, and sensory acceptance of tortilla chips made from alternative flours derived from local ingredients, including maize, beet, flaxseed, bean, and chia. Three blends were assessed: maize with beans, maize with beet, and maize with chia–flaxseed. Significant differences (p < 0.05) were observed in the flours’ moisture, ash, protein, lipid, and mineral content. Flaxseed flour exhibited the highest protein content (40.03 g/100 g), while chia flour was notable for its lipid (32.25 g/100 g) and fiber (38.51 g/100 g) content. Bean and chia flour were rich in iron and zinc. Sensory evaluations, conducted with 300 consumers in Honduras, revealed general acceptance of all blends, with maize chips enriched with chia–flaxseed showing the highest preference (47.2%). Approximately 50% of participants reported consuming tortilla chips weekly, prioritizing taste, freshness, and price. Notably, over 40% expressed willingness to pay a premium for more nutritious, baked options. These results underscore the potential of alternative flours to enhance local diets and foster healthier eating habits. Moreover, the positive consumer response highlights a significant market opportunity for small and medium-sized enterprises (SMEs), promoting awareness of nutrition and public health in Honduras. Full article

Fortification is the deliberate addition of essential micronutrients, such as vitamins and minerals, to enhance a food’s nutritional profile and contribute to public health. A promising approach to fortification involves the use of plant by-products which are rich in bioactive compounds. This study evaluates the effects of incorporating broccoli by-product powder into corn-flour tortillas. Five formulations were developed: a control (100% corn flour) and variations replacing 2.5%, 5%, 7.5%, and 10% of the corn flour with broccoli by-product powder. Adding broccoli powder resulted in darker tortillas with slightly reduced firmness. Water and oil absorption capacities increased in fortified tortillas compared to the control. The broccoli powder in the tortillas significantly enhanced their nutritional profile. Calcium content increased nearly six-fold, while potassium and iron concentrations were tripled in tortillas fortified with 10% broccoli powder. Additionally, dietary fiber content rose by 23%. Antioxidant capacity improved significantly, particularly in total polyphenol content. Fortification also led to a significantly higher glucosinolate concentrations, notably neoglucobrassicin and glucoraphanin. Sensory evaluation showed that consumers found tortillas containing 2.5% to 7.5% broccoli powder to be the most acceptable. However, fortification at 10% negatively impacted overall acceptability, primarily due to the intensified brassica flavor. In conclusion, incorporating broccoli by-product powder into corn tortillas enhanced their nutritional and functional properties, whilst retaining acceptable sensory characteristics. This approach promotes the sustainable valorization of by-products, offering a viable, eco-friendly alternative for the development of functional, nutrient-rich foods that support sustainability in the food industry. Full article

Background: This work focused on the study of the indirect fortification of Mexican tortillas made from nixtamalized masa (NM) with nixtamalized commercial corn flour (NCC-F) fortified with Zn, Fe, vitamins and folic acid. Methods: The chemical proximate values (CPV), ash content, mineral composition by inductively coupled plasma, in vitro protein digestibility (PD), protein digestibility-corrected amino acid score (PDCAAS), the total starch content, the resistant starch (RS) content in nixtamalized corn tortillas (NC-T) and nixtamalized commercial corn flour tortillas (NCCF-T) and the contribution of tortillas prepared with a mixture of NM and NCC-F (75:25 and 50:50, NM:NCC-F) to the recommended dietary intake (RDI) of minerals and vitamins were determined. Results: No significant differences (p < 0.05) were found in CPV and RS content between NCCF-T and NC-T. Ca content was significantly higher (p < 0.05) in NC-T than in NCCF-T, while Fe, K, Zn, folic acid contents, PD and PDCAAS content was higher in NCCF-T compared to NC-T (p < 0.05). The tortillas made with a mixture of NM and NCC-F (50:50) provide 43.07% of the RDI of Ca for Mexican children and adults, while ~45% and >100% of the RDI of Mg for adults and children, respectively, are provided by these tortillas. Similarly, tortillas from the NM:NCC-F mixture (50:50) provide average values of 45, 71 and ~91% of the RDI of Fe, Zn and folic acid, respectively, for all age groups of the Mexican population. Conclusions: NCCF-T contribute significantly to the recommended daily intake (RDI) of micronutrients such as iron, zinc, magnesium and folic acid, while tortillas made from the traditional nixtamalized corn of the Mexican diet have a higher calcium content. Tortillas made from a mixture of NCC-F and traditional NM may be an effective way to address micronutrient deficiencies in the Mexican population. Full article

Corn, Zea mays, is an ancestral food, culturally included in different forms in the Guatemalan diet. The most common form is in tortillas and tamales, which makes them suitable for incorporating other ingredients that increase their nutrient content. A sensory study was conducted with the aim of determining whether the appearance and texture of the tortilla and tamale remain acceptable when adding germinated chia seed flour (Salvia hispanica L). Germination was carried out for one day at 20 °C; it was prepared as flour and mixed with nixtamalized corn flour in a ratio of 10:90 and enough water to mold the tortillas; for the tamale, the same proportion of corn flour and germinated chia flour was used, and water and 8.6 percent oil were added. The tortillas and tamales were prepared and cooked in a traditional way by experts. For the acceptability test, 52 consumers were recruited, who signed the informed consent and subsequently evaluated the appearance and texture using a five-point hedonic scale (1 = I like it very much, 5 = I dislike it very much). The results indicate that the average acceptability of the appearance of the tortilla is 1.2 and the texture is 1.8. The average acceptability of the appearance of the tamale is 1.9 and the texture is 1.19. When comparing the acceptability of the appearance and texture of both preparations, a significant difference was found (p < 0.05), with the appearance and texture of the tamale being more acceptable. Full article

The rationale for this research is the investigation of the potential health benefits as well as the antibacterial and antifungal properties of selected aromatic plants from the Lamiaceae family, which may lead to the development of improved functional foods. The present study investigated the effects of incorporating dried aromatic plants Thymus vulgaris, Thymus serpyllum, Thymus × citriodorus, Origanum vulgare and Rosmarinus officinalis at a concentration of 1% in refined wheat flour and wholemeal flour on the production of functional tortillas. Sensory analysis was employed to identify the optimal 1% addition, with the objective of achieving a favorable flavor and aroma profile. It was hypothesized that this addition would affect water activity, moisture, texture, color, antioxidant content and phenolic content, thereby enhancing the tortillas as a source of bioactive compounds. The results indicated that the type of flour used had a significant impact on the water activity of the tortillas, with wholemeal flour resulting in higher water activity than refined flour. The water activity ranged between 0.735 and 0.821, while the water content remained relatively stable. The water activity in whole-grain tortillas was significantly higher than that of refined flour tortillas, with a value exceeding 0.8, which makes them susceptible to mold growth and the production of mycotoxins. The sensory evaluations indicated that the enriched refined flour tortillas with common thyme (Thymus vulgaris), lemon thyme (Thymus × citriodorus) and rosemary (Rosmarinus officinalis) were rated highly; a similar result was observed for the whole-grain tortillas enriched with wild thyme (Thymus serpyllum) and lemon thyme. The whole-grain tortillas with rosemary were rated the highest of all the tortillas. The addition of aromatic plants increased the phenolic content and the antioxidant potential, depending on the flour type and the plant used. The addition of wild thyme and rosemary resulted in a significant increase in the phenolic content of wheat tortillas, while all enriched whole-grain tortillas exhibited a higher phenolic content than the control samples. The highest phenolic content in whole-grain tortillas was found in those fortified with rosemary, oregano and wild thyme. The highest antioxidant content was recorded in tortillas prepared with rosemary, irrespective of whether the flour used was refined or wholemeal. Fourteen phenolic compounds were tentatively identified in aromatic plants tested. The main phenolic compounds in Origanum vulgare were flavonoids. Rosmarinic acid was the dominant phenolic compound in rosemary and all thyme species, reaching the highest level in rosemary. Such high levels of rosmarinic acid may be responsible for the high antioxidant and total phenolic contents observed in rosemary extracts and also in tortillas when this plant is included in the recipe. The results of this study indicate that selected aromatic plants, particularly rosemary, have the potential to be utilized as functional ingredients in bakery products. By incorporating dried aromatic plants from the Lamiaceae family into wheat flour tortillas, food manufacturers can create products that not only taste better but also provide added health benefits. The use of selected herbs can improve the nutritional profile of tortillas by increasing antioxidant properties and, due to the properties of herbs, extend the shelf life of the product. Full article

Within the realm of archaeology, the analysis of biomolecules assumes significant importance in elucidating historical dietary patterns and their implications for contemporary contexts. To achieve this, knowledge and tools of both chemistry and archaeology are essential to yield objective outcomes and conduct analyses of archaeological materials for the detection of biomolecules. Usually, only minuscule remnants of ceramic fragments are retrieved from excavations, which limits the feasibility of comprehensive laboratory analysis. This study aimed to establish a protocol for analyzing fatty acids and starch from archaeological food utensils with minimal sample quantities. Various experiments were conducted to replicate preparations that might have occurred in archaeological vessels, aiming to establish the optimal protocol. The analyses were performed using clay griddles, subjecting vegetable oil to varying temperatures for fatty acid assessment. For starch analysis, a series of experiments encompassed diverse forms of potato preparations (pulp, chuño, tortilla, carbonization, and freeze-drying) and maize (flour, tortilla, and carbonization). The verification of the experiments was confirmed by conducting identical analyses, as developed in the current study, on authentic archaeological fragments. The principal outcomes of this investigation include the successful extraction of both types of biomolecules using only 0.25 g of the sample, obtained through direct scraping from the vessel. Soxhlet extraction was identified as the most efficient strategy to recover fatty acids. Additionally, a comprehensive protocol for the identification of starch extraction was developed. This study has, for the first time, elucidated two detailed methodologies for the extraction of fatty acids and starch in scenarios in which researchers can obtain limited quantities of archaeological food utensil fragments. Full article