Search Results (56)

Fiber contamination originating from disposable dental microapplicators has received limited attention despite its potential influence on adhesive procedures. The aim of this pilot in vitro study was to evaluate fiber-like structure release associated with different microapplicator types during the application of universal adhesive systems. Three universal adhesives (Clearfil Universal Bond Quick, Gluma Universal, and G-Premio BOND) and five microapplicator types (X-Slim, Clinique, Prima, Single TIM, and ZerofloX silicone-bristle microapplicators) were evaluated. A total of 75 adhesive applications were performed on standardized sandblasted glass substrates under controlled laboratory conditions. Adhesives were actively applied for 10 s, and fiber-like structures were quantified microscopically using ImageJ software. Statistical analysis included descriptive statistics, two-way ANOVA, and Tukey post hoc testing (α = 0.05). Significant differences were observed among microapplicator types. X-Slim applicators produced the highest fiber counts, whereas Single TIM applicators demonstrated substantially lower values. No detectable fiber-like structures were observed in specimens treated with the ZerofloX silicone-bristle microapplicator. Adhesive system type showed a comparatively smaller influence on fiber counts than microapplicator design. Within the limitations of this pilot in vitro study, microapplicator type appeared to be the primary factor influencing visible fiber contamination during adhesive application. Further studies are required to determine whether the contamination patterns observed influence adhesive performance under clinically relevant conditions. Full article

Chicken manure and its potential to contaminate water systems through the dispersal of pathogenic bacteria are major concerns in environmental and public health. In this study, a metagenomic analysis was employed to systematically identify and compare bacterial assemblages in chicken manure (CM) and in a contaminated sample of chicken manure wastewater (CMW). Whole DNA was extracted from CM and CMW, followed by whole-genome shotgun sequencing; data analysis was done using online Galaxy software (ver. 26.0.1.dev1). Metagenomic analysis reveals a complex One Health challenge. Data showed that CM and CMW are different in their microbiota, as indicated by a distinct separation of beta diversity values and limited overlapping of species between sample types. In the current study, we found a greatly significant common functional set of adapted bacterial masses, including major pathogenic bacterial groups as well as opportunistic and environmental bacterial species, indicative of a direct contamination from CM and CMW. Notably, in both CM and CMW, a plethora of opportunistic, enteric, and environmental pathogens like Escherichia coli, Salmonella enterica, and Acinetobacter baumannii were found, coupled with an indication of a direct functional flow between both ecosystems as tangled reservoirs. Chicken manure samples showed differences in taxonomic composition and inferred functional profiles at the time of sampling: CM1 was pathogen-enriched, CM2 exhibited strong nitrogen-supportive metabolism, CM3 was dominated by fiber-degrading decomposers, and CM4 showed high methane-producing potential with environmental risk. Such findings underscore the raising of chickens as a potential source of harmful bacteria for the environment. It is important to note that this study represents a preliminary investigation with certain limitations, including the absence of biological replicates, lack of temporal sampling, and limited capacity to infer dynamic ecological interactions. Yet this metagenomic report is more about describing the taxonomy and functional potential of the bacteria, rather than discussing the actual ecological processes of these microorganisms in the environment. Future studies will be required to explore these aspects. Full article

Microplastics (MPs) are prevalent in coastal habitats, but their occurrence in highly vulnerable coastal zones and human exposure risk are poorly understood, especially in developing nations like Bangladesh. This inquiry focused on the prevalence and potential hazards of MPs in surface sediment and shallow groundwater samples collected from 12 sites in Cox’s Bazar, Bangladesh, from August to October 2023. Using stereomicroscopy and FTIR, MPs were quantified, with concentrations ranging from 60 to 813.33 MPs/kg in surficial sediment and 3.34 to 36.66 MPs/L in shallow groundwater, with mean values of 294.38 ± 26.61 MPs/kg and 18.91 ± 4.75 MPs/L. The dominant MPs were composed of transparent and white fibers, ranging in size from 0 to 0.5 mm, with HDPE (High-Density Polyethylene) and PP (Polypropylene) identified as the most commonly found polymers. To assess MP exposure in humans and the environment, this investigation used three indices: the polymer hazard index (PHI), the pollutant load index (PLI), and the estimated daily intake (EDI). The findings indicate that children exhibit greater exposure than adults, with observed low contamination levels, alongside a spectrum of toxicity from moderate to extreme. This study enhances understanding of MP contamination in the surficial sediments and shallow groundwater of Bangladesh, highlighting the need for further investigation into ecotoxicology, human health risks, legislation, and related issues. Full article

Microplastics are persistent contaminants in coastal wetlands, yet the mechanisms of their microbial transformation remain poorly understood. This study examined the interactions between a wetland sediment-derived microbial consortium and polyethylene terephthalate (PET) fibers over a 60-day incubation. After 60 days, the consortium caused a PET weight loss of 13.7 ± 0.9%, whereas the abiotic control showed a less than 2% loss. The water contact angle decreased from 77.5 ± 1.2° to 75.8 ± 0.4°, suggesting enhanced surface hydrophilicity. Multi-scale surface analyses (SEM, WCA, and FTIR) confirmed progressive microbial colonization, increased surface roughness, and enhanced hydrophilicity through microbially mediated modification. High-throughput 16S rRNA sequencing unveiled a distinct community succession; PET exerted selective pressure that reduced alpha-diversity while enriching specific functional taxa such as Acinetobacter and Pseudomonas. Moreover, isolation and co-culture assays confirmed the importance of synergistic microbial interactions in PET transformation, with co-culture of four representative isolates causing 9.2 ± 0.1% PET weight loss, compared with only 1.7–3.2% in monocultures. These findings underscore the intrinsic natural attenuation potential of wetland ecosystems and provide a critical scientific basis for developing nature-based management strategies. By identifying key functional taxa and PET-associated transformation pathways, this work supports the establishment of early-warning mechanisms to safeguard the ecological integrity and soil health of coastal World Natural Heritage sites like the Tiaozini Wetland. Full article

Microplastics (MPs) have emerged as a growing environmental and food safety concern, with their presence widely reported in aquatic organisms and seafood. However, their occurrence in traditionally processed and fermented fish products remains unexplored. This study provides the first evidence of MP contamination in ethnic fermented fish products of Northeast India, namely Ngari, Hentak, and Shidal. MPs were analyzed for abundance, size distribution, morphology, color, and polymer composition using microscopic examination and Laser Raman Spectroscopy. The average MP abundance was 16.50 ± 5.18 MPs/g in Ngari, 15.73 ± 4.83 MPs/g in Shidal, and 20.50 ± 3.00 MPs/g in Hentak. Fibers and fragments were the dominant morphotypes across all products, with transparent and black particles occurring most frequently. Polymer characterization revealed polyethylene (PE) and polypropylene (PP) as the predominant polymers, followed by polyamide (PA), polyvinyl chloride (PVC), and polystyrene (PS). Size distribution analysis showed that MPs in the 101–300 µm range were most abundant in Ngari and Shidal, whereas smaller MPs (<50 µm) predominated in Hentak. The use of whole fish, including the gastrointestinal tract and gills, primary sites for MP accumulation, along with non-standardized fermentation practices and atmospheric deposition during retail, likely contributes to contamination. These findings highlight an overlooked route of human exposure to MPs through traditional fermented foods and underscore the need for improved processing practices and mitigation strategies to safeguard food safety and sustainability. Full article

Microplastic (MP) contamination along coastlines is a globally recognized environmental concern. This paper investigates the seasonal and cross-shore distribution of large and small microplastics (LMPs and SMPs) at four sites along the Ferrara coast in the northern Adriatic Sea (Italy). A combination of sampling and analytical approaches was employed to characterize the typology, morphology, and size of MPs. A subsample of LMPs was analyzed by Raman spectroscopy to determine polymers’ composition. The mean abundances recorded were 5.66 ± 13.15 LMPs/m² and 2402.19 ± 1169.85 SMPs/m². Among the LMPs, pellets and fragments, essentially cream and white in color, were dominant. The samples were predominantly composed of polyethylene, polypropylene, and polyethylene terephthalate. SMPs primarily consisted of black fibers. LMPs and SMPs displayed their lowest abundances in winter and cross-shore patterns indicated preferential accumulation at dune foot and crest. Since the study sites are located downstream of the Po and Reno river mouths, urban and riverine discharges, as well as emissions from plastic-processing industries, are likely major contributors to the observed MPs. Full article

This paper proposes an adaptive layer-dependent threshold function (ALDTF) for denoising electrocardiogram (ECG) and multimode optical fiber-based cardiopulmonary signals. Based on wavelet transform, the method employs a layer-dependent threshold function strategy that utilizes the non-zero periodic peak (NZOPP) of the signal’s normalized autocorrelation function to adaptively determine the optimal threshold for each decomposition layer. The core idea applies soft thresholding at lower layers (high-frequency noise) to suppress pseudo-Gibbs oscillations, and hard thresholding at higher layers (low-frequency noise) to preserve signal amplitude and morphology. The experimental results show that for ECG signals contaminated with baseline wander (BW), electrode motion (EM) artifacts, muscle artifacts (MA), and mixed (MIX) noise, ALDTF outperforms existing methods—including SWT, DTCWT, and hybrid approaches—across multiple metrics. It achieves a ΔSNR improvement of 1.68–10.00 dB, ΔSINAD improvement of 1.68–9.98 dB, RMSE reduction of 0.02–0.56, and PRD reduction of 2.88–183.29%. The method also demonstrates excellent performance on real ECG and optical fiber cardiopulmonary signals, preserving key diagnostic features like QRS complexes and ST segments while effectively suppressing artifacts. ALDTF provides an efficient, versatile solution for physiological signal denoising with strong potential in wearable real-time monitoring systems. Full article

Microplastic pollution in freshwater systems represents a growing environmental concern, yet the dynamics of microplastic distributions in smaller tributaries like canals/creeks remain understudied. This case study presents an investigation of microplastic contamination in a canal system in upstate New York, USA, examining land use and rainfall that influence microplastic abundance, distribution, and characteristics. Water and sediment samples were collected bi-weekly (June–August 2023) from sites representing runoff from diverse land-use types: agricultural areas, residential zones, academic buildings, and parking lots. The study reveals significant land-use dependent variations in contamination, with mean concentrations of 17 ± 7 items/L in the water column, while suspended sediment and bedload reached 540 ± 230 items/kg and 370 ± 80 items/kg, respectively. Upstream water column exhibited the highest loads (27 ± 2 items/L), driven by cumulative agricultural and commercial inputs, while downstream declines highlighted vegetation-mediated sedimentation. Land-use patterns strongly influenced contamination profiles, with parking lots exhibiting tire-wear fragments, artificial turf contributing polyethylene particles, and residential areas contributing 43% textile fibers. Rainfall intensity and antecedent dry days differentially influenced transport mechanisms. Antecedent dry days strongly predicted parking lot runoff fluxes surpassing rainfall intensity effects and underscored impervious surfaces as transient microplastic reservoirs. Full article

The widespread dispersion of microplastics (MPs) has been recognized as a pervasive and persistent environmental contaminant in worldwide freshwater ecosystems, and although relative studies have skyrocketed, there are still significant knowledge gaps in areas like southern Europe. This study assesses the microplastic pollution in seven Greek inland aquatic ecosystems which vary in morphology, trophic status, and anthropogenic pressure. Surface and vertical samples were taken with 200 μm plankton nets. MPs were present in all samples, with fibers being the dominant form, having an abundance range between 0.47 and 149.4 items/m³ with fragments between 0.08 and 9.17 items/m³. Fibers and fragments had greater abundance in the vertical than in the surface samples. There were no significant abundance differences between lakes and lagoons, and among the sampling sites in each ecosystem. Blue and transparent were the colors that prevailed, and most of the fibers and fragments were smaller than 1 mm. Four types of MPs were recorded, with PET (polyethylene terephthalate) being the most frequent. The use of the novel Relative Anthropogenic Pressure (RAP) index resulted in positive correlations between certain sociological parameters and the microplastics’ abundance, efficiently reflecting the impingement of human populations on the inland aquatic ecosystems. Full article

Native plant species show significant promise for the remediation and rehabilitation of mine tailings contaminated with heavy metals (HM). Nonetheless, the harmful impact of HM can decrease plant survival, growth and reproduction, thereby diminishing the effectiveness of phytoremediation. Consequently, incorporating organic amendments into mine tailings, like biochar, can promote plant growth, decreasing the bioavailability of HM and their eventual potential to alter the food chain. This study aims to evaluate the capability of coconut fiber biochar in combination with Sanvitalia procumbens to phytostabilize HM in mine tailings by analyzing the effect of coconut fiber biochar on HM bioaccumulation levels (roots and leaves), as well as on morphological, physiological, and genotoxic parameters of S. procumbens grown in mine tailing substrate and mine tailing/biochar. Also, a physicochemical analysis of coconut fiber biochar was conducted. This research was conducted over 100 days on plants grown in greenhouse settings using two different substrates (mine tailing and agrolite [75/25 v/v] and mine tailing and coconut fiber biochar [75/25 v/v]). Every 25 days, 12 plants were selected per treatment for analysis. The bioaccumulation pattern exhibited by S. procumbens was Zn > Pb > Cu > Cd, in root and leaf tissues for both treatments. S. procumbes grown in mine tailing/biochar substrate showed the lowest HM bioaccumulation levels in both tissues in comparison to mine tailing substrate: Zn from 2.95 to 2.50 times lower; Pb 3.04 to 2.82; Cu 3.10 to 2.12; and Zn 2.12 to 3.00 in roots and leaves, respectively. The coconut fiber biochar was rich in functional groups, such as carboxyl and hydroxyl groups, which could favor HM adsorption. Immobilization percentage of HM by coconut fiber biochar showed the following pattern: Pb (66.33%) > Zn (64.50%) > Cu (62.82%) > Cd (55.39%). Incorporating coconut fiber biochar as an amendment improves HM phytostabilization efficiency by reducing their bioaccumulation, increasing biomass production and chlorophyll concentration, and reducing genetic damage levels. This strategy represents a sustainable approach towards reducing the ecological risk of HM biomagnification, alleviating the adverse effects of HM exposure on ecosystem health. Full article

Parkinson’s disease (PD) is a neurodegenerative disorder characterized by motor symptoms like tremor, rigidity, and bradykinesia. The WHO estimates that 10 million people currently have PD, with its prevalence expected to double to 20 million by 2050. Key risk factors include age, male sex, environmental contaminants, and family history. Emerging evidence links gut microbiota dysbiosis to PD, suggesting it contributes to neuroinflammation and disease progression, though the role of dietary interventions remains unclear. This study used computational simulations with genome-scale metabolic models (GEMs) to analyze how diet impacts the gut microbiota in PD patients. Fecal microbiota from PD patients and healthy controls were compared across three diets: high-fiber, Mediterranean, and vegan. Simulations revealed increased pro-inflammatory bacteria (e.g., Escherichia coli O157) in PD patients, likely due to reduced bacterial competition, alongside the decreased production of beneficial metabolites like butyrate, phenylalanine, and cysteine. The Mediterranean diet showed higher short-chain fatty acid production, potentially benefiting PD patients. These findings underscore the importance of dietary interventions in modulating the gut microbiome and suggest that targeted diets may complement PD therapies, improving patient outcomes. Full article

Residual and waste materials such as biowaste and the perennial energy crop Silphium perfoliatum (cup-plant) contain high fiber contents, which limit their energetic utilization in biogas plants. Pre-separating the fiber fraction can improve overall valorization. The recovered natural fibers can be further used as raw materials, e.g., in paper production or fiber-reinforced composites. This study aimed to optimize fiber extraction from biogenic residues and renewable raw materials using pilot-scale Thermal-pressure hydrolysis (TPH). Biowaste and cup-plant were used as substrates. Process parameters (150, 160, 170 °C; 15, 30, 60 min) were systematically varied to evaluate their influence on process efficiency, chemical composition, and functional properties of the resulting fiber and pulp fractions. Biowaste and cup-plant produced final products with similar dry matter (DM) contents—fibers (~36% DM) and pulp (~3.2% DM)—but differed markedly in chemical composition: biowaste was richer in nutrients, whereas the cup plant contained more fiber. Sugar release from the cup-plant increased by over 1900% during TPH and, like the organic acids, was largely relocated to the pulp fraction. Methane yields of the resulting pulps ranged between 310 and 375 L_CH4kg_ODM⁻¹, significantly higher than those measured in the fiber fractions, which ranged from 180 to 250 L_CH4kg_ODM⁻¹. Approximately 55% of the total energy potential was transferred into the pulp. Despite the formation of organic acids and potential inhibitors during TPH, no critical threshold values were exceeded. The energy balance of the Biowaste fiber processing was neutral (biowaste: energy demand 475 kWh/t, energy yield from biogas 484 kWh/t). For papermaking applications, the cup-plant proved to be significantly more suitable, as the heterogeneity and contamination of biowaste limited its material usability. The results highlight the potential of TPH for the combined energetic and material utilization of biogenic residues. Full article

Date palm (Phoenix dactylifera L.) is a vital crop cultivated primarily in developing regions, playing a strategic role in global food security through its significant contribution to nutrition, economy, and livelihoods. Global and regional production trends revealed increasing demand and expanded cultivation areas, underpinning the fruit’s importance in national food security policies and economic frameworks. The date fruit’s rich nutritional profile, encompassing carbohydrates, dietary fiber, minerals, and bioactive compounds, supports its status as a functional food with health benefits. Postharvest technologies and quality preservation strategies, including temperature-controlled storage, advanced drying, edible coatings, and emerging AI-driven monitoring systems, are critical to reducing losses and maintaining quality across diverse cultivars and maturity stages. Processing techniques such as drying, irradiation, and cold plasma distinctly influence sugar composition, texture, polyphenol retention, and sensory acceptance, with cultivar- and stage-specific responses guiding optimization efforts. The cold chain and innovative packaging solutions, including vacuum and modified atmosphere packaging, along with biopolymer-based edible coatings, enhance storage efficiency and microbial safety, though economic and practical constraints remain, especially for smallholders. Microbial contamination, a major challenge in date fruit storage and export, is addressed through integrated preservation approaches combining thermal, non-thermal, and biopreservative treatment. However, gaps in microbial safety data, mycotoxin evaluation, and regulatory harmonization hinder broader application. Date fruit derivatives such as flesh, syrup, seeds, press cake, pomace, and vinegar offer versatile functional roles across food systems. They improve nutritional value, sensory qualities, and shelf life in bakery, dairy, meat, and beverage products while supporting sustainable waste valorization. Emerging secondary derivatives like powders and extracts further expand the potential for clean-label, health-promoting applications. This comprehensive review underscores the need for multidisciplinary research and development to advance sustainable production, postharvest management, and value-added utilization of date palm fruits, fostering enhanced food security, economic benefits, and consumer health worldwide. Full article

Bambara groundnut (Vigna subterranea), a vital yet underutilized African legume, significantly boosts food security due to its nutritional value and adaptability to harsh climates and soils. However, its processing yields substantial waste like husks, shells, and haulms, which are often carelessly discarded, causing environmental damage. This paper highlights the urgent need to valorize these waste streams to unlock sustainable growth and economic development. Given their lignocellulosic composition, Bambara groundnut residues are ideal for generating biogas and bioethanol. Beyond energy, these wastes can be transformed into various bio-based products, including adsorbents for heavy metal removal, activated carbon for water purification, and bioplastics. Their inherent nutritional content also allows for the extraction of valuable components like dietary fiber, protein concentrates, and phenolic compounds for food products or animal feed. The nutrient-rich organic matter can also be composted into fertilizer, improving soil fertility. These valorization strategies offer multiple benefits, such as reduced waste, less environmental contamination, and lower greenhouse gas emissions, alongside new revenue streams for agricultural producers. This integrated approach aligns perfectly with circular economy principles, promoting resource efficiency and maximizing agricultural utility. Despite challenges like anti-nutritional factors and processing costs, strategic investments in technology, infrastructure, and supportive policies can unlock Bambara groundnut’s potential for sustainable innovation, job creation, and enhanced food system resilience across Africa and globally. Ultimately, valorizing Bambara groundnut waste presents a transformative opportunity for sustainable growth and improved food systems, particularly within African agriculture. Full article

Carbon fiber (CF) and carbon fiber-reinforced polymers (CFRPs) are widely used in the aerospace, automotive, and energy sectors due to their high strength, stiffness, and low density. However, significant waste is generated during manufacturing and after the use of CFRPs. Traditional disposal methods like landfilling and incineration are unsustainable. CFRP machining processes, such as drilling and milling, produce fine chips and dust that are difficult to recycle due to their heterogeneity and contamination. This study investigates the oxidation behavior of CFRP drilling waste from two types of materials (tube and plate) under oxidative (non-inert) conditions. Thermogravimetric analysis (TGA) was performed from 200 °C to 800 °C to assess weight loss related to polymer degradation and carbon fiber integrity. Scanning electron microscopy (SEM) was used to analyze morphological changes and fiber damage. The optimal range for removing the polymer matrix without significant fiber degradation has been identified as 500–600 °C. At temperatures above 700 °C, notable surface and internal fiber damage occurred, along with nanostructure formation, which may pose health and environmental risks. The results show that partial fiber recovery is possible under ambient conditions, and this must be considered regarding the harmful risks to the human body if submicron particles are inhaled. This research supports sustainable CFRP recycling and fire hazard mitigation. Full article