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17 pages, 1459 KiB  
Article
Assessing Controlled Traffic Farming as a Precision Agriculture Strategy for Minimising N2O Losses
by Bawatharani Raveendrakumaran, Miles Grafton, Paramsothy Jeyakumar, Peter Bishop and Clive Davies
Nitrogen 2025, 6(3), 63; https://doi.org/10.3390/nitrogen6030063 - 4 Aug 2025
Abstract
Intensive vegetable farming emits high nitrous oxide (N2O) due to traffic-induced compaction, highlighting the need for preventing nitrogen (N) losses through better traffic management. This study examined the effects of Controlled Traffic Farming (CTF) and Random Traffic Farming (RTF) on N [...] Read more.
Intensive vegetable farming emits high nitrous oxide (N2O) due to traffic-induced compaction, highlighting the need for preventing nitrogen (N) losses through better traffic management. This study examined the effects of Controlled Traffic Farming (CTF) and Random Traffic Farming (RTF) on N2O emissions using intact soil cores (diameter: 18.7 cm; depth: 25 cm) collected from a vegetable production system in Pukekohe, New Zealand. Soil cores from CTF beds, CTF tramlines, and RTF plots were analysed under fertilised (140 kg N/ha) and unfertilised conditions. N2O fluxes were monitored over 58 days using gas chambers. The fertilised RTF system significantly (p < 0.05) increased N2O emissions (5.4 kg N2O–N/ha) compared to the unfertilised RTF system (1.53 kg N2O–N/ha). The emission from fertilised RTF was 46% higher than the maximum N2O emissions (3.7 kg N2O–N/ha) reported under New Zealand pasture conditions. The fertilised CTF system showed a 31.6% reduction in N2O emissions compared to fertilised RTF and did not differ significantly from unfertilised CTF. In general, CTF has demonstrated some resilience against fertiliser-induced N2O emissions, indicating the need for further investigation into its role as a greenhouse gas mitigation strategy. Full article
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24 pages, 913 KiB  
Article
Fermentation Efficiency and Profile of Volatile Compounds in Rye Grain Mashes from Crops Fertilised with Agrifood Waste Ashes
by Łukasz Ściubak, Andrzej Baryga, Maria Balcerek, Katarzyna Pielech-Przybylska, Urszula Dziekońska-Kubczak and Stanisław Brzeziński
Molecules 2025, 30(15), 3251; https://doi.org/10.3390/molecules30153251 - 2 Aug 2025
Viewed by 162
Abstract
The utilisation of agrifood waste ashes has the potential to enhance the nutrient content of cereal crops, thereby optimising both yield and grain quality. This study investigated rye grain composition, the fermentation efficiency, and volatile compounds in mashes made from crops fertilised with [...] Read more.
The utilisation of agrifood waste ashes has the potential to enhance the nutrient content of cereal crops, thereby optimising both yield and grain quality. This study investigated rye grain composition, the fermentation efficiency, and volatile compounds in mashes made from crops fertilised with agrifood waste ashes derived from the combustion of corn cob, wood chips, and biomass with defecation lime. The ashes were applied at 2, 4, and 8 t/ha, separately and as mixtures of corn cob (25%) with wood chips (75%) and corn cob (50%) with biomass and defecation lime (50%). Rye mashes were prepared using the pressureless starch liberation method. The starch content in the majority of the rye grains was comparable to the control sample (57.12 g/100 g). The range of ethanol concentrations observed in the fermented mashes was from 55.55 to 68.12 g/L, which corresponded to fermentation yields of 67.25–76.59% of theoretical. The lowest fermentation yield was exhibited by the mash derived from rye cultivated on soil fertilised with a 50:50 mixture of ashes from corn cob and biomass with defecation lime at 8 t/ha. This mash contained more than double the acetaldehyde concentration and total aldehyde content compared to the other samples. These findings demonstrate the potential of using waste biomass ash as a source of macro- and microelements for rye cultivation, enabling the production of agricultural distillates. To ensure high fermentation efficiency and low aldehyde levels, ash dosage and composition need to be established based on experimental optimisation. Full article
(This article belongs to the Section Food Chemistry)
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21 pages, 3648 KiB  
Article
Preparation and Physicochemical Evaluation of Ionically Cross-Linked Chitosan Nanoparticles Intended for Agricultural Use
by Maria Karayianni, Emi Haladjova, Stanislav Rangelov and Stergios Pispas
Polysaccharides 2025, 6(3), 67; https://doi.org/10.3390/polysaccharides6030067 - 1 Aug 2025
Viewed by 178
Abstract
The search for sustainable, economically viable, and effective plant protection strategies against pathogenic bacteria, fungi, and viruses is a major challenge in modern agricultural practices. Chitosan (CS) is an abundant cationic natural biopolymer known for its biocompatibility, low toxicity, and antimicrobial properties. Its [...] Read more.
The search for sustainable, economically viable, and effective plant protection strategies against pathogenic bacteria, fungi, and viruses is a major challenge in modern agricultural practices. Chitosan (CS) is an abundant cationic natural biopolymer known for its biocompatibility, low toxicity, and antimicrobial properties. Its potential use in agriculture for pathogen control is a promising alternative to traditional chemical fertilisers and pesticides, which raise concerns regarding public health, environmental protection, and pesticide resistance. This study focused on the preparation of chitosan nanoparticles (CS-NPs) through cross-linking with organic molecules, such as tannic acid (TA). Various formulations were explored for the development of stable nanoscale particles having encapsulation capabilities towards low compounds of varying polarity and with potential agricultural applications relevant to plant health and growth. The solution properties of the NPs were assessed using dynamic and electrophoretic light scattering (DLS and ELS); their morphology was observed through atomic force microscopy (AFM), while analytical ultracentrifugation (AUC) measurements provided insights into their molar mass. Their properties proved to be primarily influenced by the concentration of CS, which significantly affected its intrinsic conformation. Additional structural insights were obtained via infrared and UV–Vis spectroscopic measurements, while detailed fluorescence analysis with the use of three different probes, as model cargo molecules, provided information regarding the hydrophobic and hydrophilic microdomains within the particles. Full article
(This article belongs to the Collection Bioactive Polysaccharides)
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19 pages, 3489 KiB  
Article
Impact of Nitrogen Fertilisation and Inoculation on Soybean Nodulation, Nitrogen Status, and Yield in a Central European Climate
by Waldemar Helios, Magdalena Serafin-Andrzejewska, Marcin Kozak and Sylwia Lewandowska
Agriculture 2025, 15(15), 1654; https://doi.org/10.3390/agriculture15151654 - 1 Aug 2025
Viewed by 178
Abstract
Soybean (Glycine max [L.] Merr.) cultivation is expanding in Central Europe due to the development of early-maturing cultivars and growing demand for plant-based protein produced without the use of genetically modified organisms. However, nitrogen (N) management remains a major challenge in temperate [...] Read more.
Soybean (Glycine max [L.] Merr.) cultivation is expanding in Central Europe due to the development of early-maturing cultivars and growing demand for plant-based protein produced without the use of genetically modified organisms. However, nitrogen (N) management remains a major challenge in temperate climates, where variable weather conditions can significantly affect nodulation and yield. This study evaluated the effects of three nitrogen fertilisation doses (0, 30, and 60 kg N·ha−1), applied in the form of ammonium nitrate (34% N) and two commercial rhizobial inoculants—HiStick Soy (containing Bradyrhizobium japonicum strain 532C) and Nitragina (including a Polish strain of B. japonicum)—on nodulation, nitrogen uptake, and seed yield. A three-year field experiment (2017–2019) was conducted in southwestern Poland using a two-factor randomized complete block design. Nodulation varied significantly across years, with the highest values recorded under favourable early-season moisture and reduced during drought. In the first year, inoculation with HiStick Soy significantly increased nodule number and seed yield compared to Nitragina and the uninoculated control. Nitrogen fertilisation consistently improved seed yield, although it had no significant effect on nodulation. The highest nitrogen use efficiency was observed with moderate nitrogen input (30 kg N·ha−1) combined with inoculation. These findings highlight the importance of integrating effective rhizobial inoculants with optimized nitrogen fertilisation to improve soybean productivity and nitrogen efficiency under variable temperate climate conditions. Full article
(This article belongs to the Special Issue Strategies to Enhance Nutrient Use Efficiency and Crop Nutrition)
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33 pages, 872 KiB  
Review
Implications of Fertilisation on Soil Nematode Community Structure and Nematode-Mediated Nutrient Cycling
by Lilian Salisi Atira and Thomais Kakouli-Duarte
Crops 2025, 5(4), 50; https://doi.org/10.3390/crops5040050 - 30 Jul 2025
Viewed by 186
Abstract
Soil nematodes are essential components of the soil food web and are widely recognised as key bioindicators of soil health because of their sensitivity to environmental factors and disturbance. In agriculture, many studies have documented the effects of fertilisation on nematode communities and [...] Read more.
Soil nematodes are essential components of the soil food web and are widely recognised as key bioindicators of soil health because of their sensitivity to environmental factors and disturbance. In agriculture, many studies have documented the effects of fertilisation on nematode communities and explored their role in nutrient cycling. Despite this, a key gap in knowledge still exists regarding how fertilisation-induced changes in nematode communities modify their role in nutrient cycling. We reviewed the literature on the mechanisms by which nematodes contribute to nutrient cycling and on how organic, inorganic, and recycling-derived fertilisers (RDFs) impact nematode communities. The literature revealed that the type of organic matter and its C:N ratio are key factors shaping nematode communities in organically fertilised soils. In contrast, soil acidification and ammonium suppression have a greater influence in inorganically fertilised soils. The key sources of variability across studies include differences in the amount of fertiliser applied, the duration of the fertiliser use, management practices, and context-specific factors, all of which led to differences in how nematode communities respond to both fertilisation regimes. The influence of RDFs on nematode communities is largely determined by the fertiliser’s origin and its chemical composition. While fertilisation-induced changes in nematode communities affect their role in nutrient cycling, oversimplifying experiments makes it difficult to understand nematodes’ functions in these processes. The challenges and knowledge gaps for further research to understand the effects of fertilisation on soil nematodes and their impact on nutrient cycling have been highlighted in this review to inform sustainable agricultural practices. Full article
(This article belongs to the Topic Soil Health and Nutrient Management for Crop Productivity)
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25 pages, 3891 KiB  
Review
The Carbon Footprint of Milk Production on a Farm
by Mariusz Jerzy Stolarski, Kazimierz Warmiński, Michał Krzyżaniak, Ewelina Olba-Zięty and Paweł Dudziec
Appl. Sci. 2025, 15(15), 8446; https://doi.org/10.3390/app15158446 - 30 Jul 2025
Viewed by 289
Abstract
The environmental impact of milk production, particularly its share of greenhouse gas (GHG) emissions, is a topic under investigation in various parts of the world. This paper presents an overview of current knowledge on the carbon footprint (CF) of milk production at the [...] Read more.
The environmental impact of milk production, particularly its share of greenhouse gas (GHG) emissions, is a topic under investigation in various parts of the world. This paper presents an overview of current knowledge on the carbon footprint (CF) of milk production at the farm level, with a particular focus on technological, environmental and organisational factors affecting emission levels. The analysis is based on a review of, inter alia, 46 peer-reviewed publications and 11 environmental reports, legal acts and databases concerning the CF in different regions and under various production systems. This study identifies the main sources of emissions, including enteric fermentation, manure management, and the production and use of feed and fertiliser. It also demonstrates the significant variability of the CF values, which range, on average, from 0.78 to 3.20 kg CO2 eq kg−1 of milk, determined by the farm scale, nutritional strategies, local environmental and economic determinants, and the methodology applied. Moreover, this study stresses that higher production efficiency and integrated farm management could reduce the CF per milk unit, with further intensification having, however, diminishing effects. The application of life cycle assessment (LCA) methods is essential for a reliable assessment and comparison of the CF between systems. Ultimately, an effective CF reduction requires a comprehensive approach that combines improved nutritional practices, efficient use of resources, and implementation of technological innovations adjusted to regional and farm-specific determinants. The solutions presented in this paper may serve as guidelines for practitioners and decision-makers with regard to reducing GHG emissions. Full article
(This article belongs to the Special Issue Environmental Management in Milk Production and Processing)
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17 pages, 1473 KiB  
Article
Stimulation of Maize Growth and Development and Improvement of Soil Properties Using New Specialised Organic-Mineral Materials
by Marzena S. Brodowska, Mirosław Wyszkowski and Ryszard Grzesik
Molecules 2025, 30(14), 3050; https://doi.org/10.3390/molecules30143050 - 21 Jul 2025
Viewed by 215
Abstract
The use of mineral fertilisers has increased in recent years, but this has had a negative effect on the environment, including causing the water in rivers and lakes to become too rich in nutrients, a process known as eutrophication. Current research focuses on [...] Read more.
The use of mineral fertilisers has increased in recent years, but this has had a negative effect on the environment, including causing the water in rivers and lakes to become too rich in nutrients, a process known as eutrophication. Current research focuses on producing fertiliser materials that are environmentally friendly. The aim of this study was to examine the impact of novel specialised organic-mineral fertilisers (OMFs: NP 24-12, NP 10-10, and NP 10-10 Zn+) on the yield and chemical composition of maize. These fertilisers were compared with a control (no fertiliser) and with other fertilisers (mixture of commercial fertilisers (MCFs): NP 24-12 and NP 10-10) that were used as a reference. All fertilisers increased the SPAD index at the fifth leaf unfolded stage of maize, with the majority (apart from OMF NP 10-10) also increasing it at the panicle emergence stage. MCF NP 10-10 had the most positive effect on the plant height, while OMF NP 10-10 had the least positive effect. All fertilisers had a positive effect on maize growth and development, with MCFs NP 10-10 and NP 24-12 having by far the strongest effect on increasing crop yields. The yield of plants fertilised with OMFs NP 24-12, NP 10-10, and NP 10-10 Zn+ was lower than the yields of plants fertilised with MCF NP 24-12 and MCF NP 10-10. OMF NP 10-10 caused a greater increase in the contents of all elements, and OMF NP 24-12 caused a greater increase in most elements (except P and Ca) in maize than MCFs did at an identical NP ratio. OMF NP 10-10 Zn+ was found to have a significant impact on the mineral composition of maize, resulting in a decline in Ca and P levels, along with a notable increase in Mg, Zn, and Cu concentrations. The most significant differences were observed for Cu and Zn. The OMFs, notably NP 24-12 and NP 10-10, exhibited a comparatively diminished acidifying impact in comparison with the MCFs. The application of fertilisers resulted in a significant increase in soil nutrient levels, with most fertilisers increasing the soil N, P, and Zn contents. Additionally, the OMFs led to an increase in Cu. However, MCFs NP 24-12 and NP 10-10 reduced the soil Cu and Mn contents. Studies should include other species as they have different needs. Field experiments are also needed. Full article
(This article belongs to the Special Issue Applied Chemistry in Europe)
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16 pages, 2557 KiB  
Article
Surveillance of Salmonella Serovars in the Food Chain in Poland: A Five-Year Review (2016–2020)
by Ewelina Skrzypiec, Magdalena Skarżyńska, Magdalena Zając, Renata Kwit, Anna Lalak, Aleksandra Śmiałowska-Węglińska, Emilia Mikos-Wojewoda, Paulina Pasim, Weronika Koza, Dominika Wojdat, Inga Bona, Dominika Pastuszka, Sylwia Hudzik-Pałosz and Dariusz Wasyl
Pathogens 2025, 14(7), 712; https://doi.org/10.3390/pathogens14070712 - 18 Jul 2025
Viewed by 292
Abstract
(1) Background: Understanding the distribution of Salmonella serovars in food, animals, and their environments is crucial for identifying infection sources and monitoring pathogen prevalence in the food chain. This study analysed Salmonella serovars in Poland from 2016 to 2020, focusing on their epidemiological [...] Read more.
(1) Background: Understanding the distribution of Salmonella serovars in food, animals, and their environments is crucial for identifying infection sources and monitoring pathogen prevalence in the food chain. This study analysed Salmonella serovars in Poland from 2016 to 2020, focusing on their epidemiological significance. (2) Methods: Isolation of Salmonella was carried out following PN-EN ISO 6579 standards, and serotyping was performed using the White–Kauffmann–Le Minor scheme. A total of 7104 isolates were collected from food-producing animals, their environments, food of animal origin, feedingstuffs, and fertilisers. (3) Results: A total of 175 serovars were identified, with S. Enteritidis (n = 2905; 40.9%), S. Infantis (n = 1167; 16.4%), and S. Typhimurium (n = 360; 5.1%) being the most prevalent. Species-specific patterns were observed: S. Enteritidis dominated in chickens, ducks, and cattle; S. Kentucky in turkeys; S. Typhimurium in geese; and monophasic S. Typhimurium in pigs. S. Enteritidis and S. Infantis were most frequent in food of animal origin, especially broiler meat. In feedingstuffs, S. Agona was predominant, while fertilisers mostly contained S. Derby and S. Infantis. (4) Conclusions: The study highlights the source-dependent variety of Salmonella serovars and the importance of serotyping in tracing infection routes and preventing the spread of pathogens. Identifying the most common serovars supports the development of targeted preventive measures, including improved biosecurity, hygiene, and management practices to enhance food safety. Full article
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18 pages, 3353 KiB  
Article
An Evaluation of a Novel Air Pollution Abatement System for Ammonia Emissions Reduction in a UK Livestock Building
by Andrea Pacino, Antonino La Rocca, Donata Magrin and Fabio Galatioto
Atmosphere 2025, 16(7), 869; https://doi.org/10.3390/atmos16070869 - 17 Jul 2025
Viewed by 323
Abstract
Agriculture and animal feeding operations are responsible for 87% of ammonia emissions in the UK. Controlling NH3 concentrations below 20 ppm is crucial to preserve workers’ and livestock’s well-being. Therefore, ammonia control systems are required for maintaining adequate air quality in livestock [...] Read more.
Agriculture and animal feeding operations are responsible for 87% of ammonia emissions in the UK. Controlling NH3 concentrations below 20 ppm is crucial to preserve workers’ and livestock’s well-being. Therefore, ammonia control systems are required for maintaining adequate air quality in livestock facilities. This study assessed the ammonia reduction efficiency of a novel air pollution abatement (APA) system used in a pig farm building. The monitoring duration was 11 weeks. The results were compared with the baseline from a previous pig cycle during the same time of year in 2023. A ventilation-controlled room was monitored during a two-phase campaign, and the actual ammonia concentrations were measured at different locations within the site and at the inlet/outlet of the APA system. A 98% ammonia reduction was achieved at the APA outlet through NH3 absorption in tap water. Ion chromatography analyses of farm water samples revealed NH3 concentrations of up to 530 ppm within 83 days of APA operation. Further scanning electron microscopy and energy-dispersive X-ray inspections revealed the presence of salts and organic/inorganic matter in the solid residues. This research can contribute to meeting current ammonia regulations (NECRs), also by reusing the process water as a potential nitrogen fertiliser in agriculture. Full article
(This article belongs to the Special Issue Impacts of Anthropogenic Emissions on Air Quality)
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23 pages, 1142 KiB  
Review
Impact of Nitrogen Fertiliser Usage in Agriculture on Water Quality
by Opeyemi Adebanjo-Aina and Oluseye Oludoye
Pollutants 2025, 5(3), 21; https://doi.org/10.3390/pollutants5030021 - 14 Jul 2025
Viewed by 433
Abstract
Agriculture relies on the widespread application of nitrogen fertilisers to improve crop yields and meet the demands of a growing population. However, the excessive use of these fertilisers has led to significant water quality challenges, posing risks to aquatic life, ecosystems, and human [...] Read more.
Agriculture relies on the widespread application of nitrogen fertilisers to improve crop yields and meet the demands of a growing population. However, the excessive use of these fertilisers has led to significant water quality challenges, posing risks to aquatic life, ecosystems, and human health. This study examines the relationship between synthetic nitrogen fertiliser usage and water pollution while identifying gaps in existing research to guide future studies. A systematic search across databases (Scopus, Web of Science, and Greenfile) identified 18 studies with quantitative data, synthesised using a single-group meta-analysis of means. As the data were continuous, the mean was used as the effect measure, and a random-effects model was applied due to varied study populations, with missing data estimated through statistical assumptions. The meta-analysis found an average nitrate concentration of 34.283 mg/L (95% confidence interval: 29.290–39.276), demonstrating the significant impact of nitrogen fertilisers on water quality. While this average remains marginally below the thresholds set by the World Health Organization (50 mg/L NO3) and EU Nitrate Directive, it exceeds the United States Environmental Protection Agency limit (44.3 mg/L NO3), signalling potential health risks, especially in vulnerable or unregulated regions. The high observed heterogeneity (I2 = 100%) suggests that factors such as soil type, agricultural practices, application rate, and environmental conditions influence nitrate levels. While agriculture is a key contributor, other anthropogenic activities may also affect nitrate concentrations. Future research should comprehensively assess all influencing factors to determine the precise impact of nitrogen fertilisers on water quality. Full article
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24 pages, 336 KiB  
Review
Molecular Shadows of Per- and Polyfluoroalkyl Substances (PFASs): Unveiling the Impact of Perfluoroalkyl Substances on Ovarian Function, Polycystic Ovarian Syndrome (PCOS), and In Vitro Fertilization (IVF) Outcomes
by Charalampos Voros, Diamantis Athanasiou, Ioannis Papapanagiotou, Despoina Mavrogianni, Antonia Varthaliti, Kyriakos Bananis, Antonia Athanasiou, Aikaterini Athanasiou, Georgios Papadimas, Athanasios Gkirgkinoudis, Kyriaki Migklis, Dimitrios Vaitsis, Aristotelis-Marios Koulakmanidis, Charalampos Tsimpoukelis, Sofia Ivanidou, Anahit J. Stepanyan, Maria Anastasia Daskalaki, Marianna Theodora, Panagiotis Antsaklis, Dimitrios Loutradi and Georgios Daskalakisadd Show full author list remove Hide full author list
Int. J. Mol. Sci. 2025, 26(14), 6604; https://doi.org/10.3390/ijms26146604 - 10 Jul 2025
Viewed by 567
Abstract
Per- and polyfluoroalkyl substances (PFASs) comprise a diverse array of synthetic chemicals that resist environmental degradation. They are increasingly recognised as endocrine-disrupting compounds (EDCs). These chemicals, found in non-stick cookware, food packaging, and industrial waste, accumulate in human tissues and fluids, raising substantial [...] Read more.
Per- and polyfluoroalkyl substances (PFASs) comprise a diverse array of synthetic chemicals that resist environmental degradation. They are increasingly recognised as endocrine-disrupting compounds (EDCs). These chemicals, found in non-stick cookware, food packaging, and industrial waste, accumulate in human tissues and fluids, raising substantial concerns regarding their impact on female reproductive health. Epidemiological studies have demonstrated associations between PFAS exposure and reduced fertility; nevertheless, the underlying molecular pathways remain inadequately understood. This narrative review investigates the multifaceted effects of PFASs on ovarian physiology, including its disruption of the hypothalamic–pituitary–ovarian (HPO) axis, alteration of anti-Müllerian hormone (AMH) levels, folliculogenesis, and gonadotropin receptor signalling. Significant attention is directed towards the emerging association between PFASs and polycystic ovarian syndrome (PCOS), wherein PFAS-induced hormonal disruption may exacerbate metabolic issues and elevated androgen levels. Furthermore, we analyse the current data regarding PFAS exposure in women undergoing treatment based on assisted reproductive technologies (ARTs), specifically in vitro fertilisation (IVF), highlighting possible associations with diminished oocyte quality, suboptimal embryo development, and implantation failure. We examine potential epigenetic and transgenerational alterations that may influence women’s reproductive capabilities over time. This study underscores the urgent need for further research and regulatory actions to tackle PFAS-related reproductive toxicity, particularly in vulnerable populations, such as women of reproductive age and those receiving fertility treatments. Full article
(This article belongs to the Special Issue Molecular Advances in Obstetrical and Gynaecological Disorders)
15 pages, 2891 KiB  
Article
Polysaccharide Hydrogels with Waste Wool Fibre as Matrix for Potential Use as CRF Fertiliser
by Ewa Szczepanik, Edyta Molik and Kinga Pielichowska
Molecules 2025, 30(13), 2885; https://doi.org/10.3390/molecules30132885 - 7 Jul 2025
Viewed by 282
Abstract
At a time of climate change, farmers face difficulties in providing food for a growing population. This results in the overuse of water and fertilisers. The aim of the research was to test the possibility of introducing waste sheep wool fibres into a [...] Read more.
At a time of climate change, farmers face difficulties in providing food for a growing population. This results in the overuse of water and fertilisers. The aim of the research was to test the possibility of introducing waste sheep wool fibres into a hydrogel to obtain a stable material that could improve water retention and could serve as a fertiliser material matrix. Wool fibres and hydrogel were chosen because of their ability to store water and their degradability. An evaluation of the swelling degree of different alginate-based hydrogel matrices was performed to select the matrix. The stability and water bonding of hydrogels with different wool fibre content were analysed and evaluated by thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The microstructure and the effect of fibres on the uniformity of the hydrogel were assessed using SEM and optical microscopy. The degree of water retention in the soil was also evaluated. The results showed that it is possible to incorporate wool fibres into the hydrogel matrix and the wool fibres make the composite porous, which allows water penetration into the material much more easily. This research has shown the possibility of using waste wool fibres as an active ingredient in sustainable fertiliser materials. Full article
(This article belongs to the Special Issue Bio-Based Polymers for Sustainable Future)
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15 pages, 3453 KiB  
Article
Feasibility of Struvite Crystals Obtained from Swine Wastewater and Urban Sewage Sludge Liquid Fraction as Fertilising Product
by Rubén Rodríguez-Alegre, Sergi Durán-Videra, Manuel Cepero-Sánchez, Laura Pérez Megías, Carlos Andecochea Saiz and Xialei You
Sustainability 2025, 17(13), 6080; https://doi.org/10.3390/su17136080 - 2 Jul 2025
Viewed by 406
Abstract
Increasing global food demand has led to an intensive use of synthetic fertilisers. In this regard, the use of non-conventional streams such as swine wastewater (SW) and urban sewage sludge liquid fraction (USS) for the production of bio-based fertilisers can increase the sustainability [...] Read more.
Increasing global food demand has led to an intensive use of synthetic fertilisers. In this regard, the use of non-conventional streams such as swine wastewater (SW) and urban sewage sludge liquid fraction (USS) for the production of bio-based fertilisers can increase the sustainability of both the fertiliser industry and agriculture while reducing the reliance on imported nutrients. In this work, USS and SW were assessed for the production of struvite at different PO43−:Mg2+ ratios. Significant differences were found in terms of struvite crystals’ shape and size among both feedstocks due to the different saturation indexes, and it was concluded that PO43−:Mg2+ ratios of 1:2 for SW and 1:1 for USS were the most suitable for obtaining big crystals suitable to be used for direct fertilisation. In addition, it was observed that the crystallisation process is highly dependent on the presence of interfering ions (mainly Ca) that can result in the formation of hydroxyapatite instead of struvite. Finally, recovering struvite from SW and USS could potentially reduce the European import of P by up to 6.5%. Full article
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17 pages, 541 KiB  
Article
Multi-Sensor Comparison for Nutritional Diagnosis in Olive Plants: A Machine Learning Approach
by Catarina Manuelito, João de Deus, Miguel Damásio, André Leitão, Luís Alcino Conceição, Rocío Arias-Calderón, Carla Inês, António Manuel Cordeiro, Eduardo Fernandes, Luís Albino, Miguel Barbosa, Filipe Fonseca and José Silvestre
Appl. Biosci. 2025, 4(3), 32; https://doi.org/10.3390/applbiosci4030032 - 2 Jul 2025
Viewed by 276
Abstract
The intensification of olive growing has raised environmental concerns, particularly regarding nutrient loss from excessive fertiliser use. In line with the European Union’s Farm to Fork strategy, which aims to halve the soil nutrient losses by 2030, this study evaluates the effectiveness of [...] Read more.
The intensification of olive growing has raised environmental concerns, particularly regarding nutrient loss from excessive fertiliser use. In line with the European Union’s Farm to Fork strategy, which aims to halve the soil nutrient losses by 2030, this study evaluates the effectiveness of two sensor-based approaches—proximal sensing with a FLAME spectrometer and remote sensing via UAV-mounted multispectral imaging—compared with foliar chemical analyses as the reference standard, for diagnosing the nutritional status of olive trees. The research was conducted in Elvas, Portugal, between 2022 and 2023, across three olive cultivars (‘Azeiteira’, ‘Arbequina’, and ‘Koroneiki’) subjected to different fertilisation regimes. Machine learning (ML) models showed strong correlations between sensor data and nutrient levels: the multispectral sensor performed best for phosphorus (P) (determination coefficient [R2] = 0.75) and potassium (K) (R2 = 0.73), while the FLAME spectrometer was more accurate for nitrogen (N) (R2 = 0.64). These findings underscore the potential of sensor-based technologies for non-destructive, real-time nutrient monitoring, with each sensor offering specific strengths depending on the target nutrient. This work contributes to more sustainable and data-driven fertilisation strategies in precision agriculture. Full article
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19 pages, 7987 KiB  
Article
Nitrogen-Induced Soil Acidification Reduces Soil Carbon Persistence by Shifting Microbial Keystone Taxa and Increasing Calcium Leaching
by Ding Wang, Jie Wang, Yuting Zhang, Xinping Chen, Ji Chen and Xiaojun Shi
Agronomy 2025, 15(7), 1586; https://doi.org/10.3390/agronomy15071586 - 29 Jun 2025
Viewed by 554
Abstract
Anthropogenic nitrogen (N) enrichment alters soil biotic (e.g., microbial metabolism) and abiotic (e.g., pH and mineralogy) properties, substantially affecting the persistence and storage of soil organic carbon (SOC). However, the response of relatively persistent mineral-associated organic carbon (MAOC) to N enrichment and the [...] Read more.
Anthropogenic nitrogen (N) enrichment alters soil biotic (e.g., microbial metabolism) and abiotic (e.g., pH and mineralogy) properties, substantially affecting the persistence and storage of soil organic carbon (SOC). However, the response of relatively persistent mineral-associated organic carbon (MAOC) to N enrichment and the underlying mechanisms are not well understood, leading to significant uncertainties regarding SOC stability under continuous N input. Based on a 15-year field N fertilisation experiment (0, 28.5, 60.0, 72.0 g N m−2 yr−1), we studied the responses of MAOC to N input and the associated changes in soil mineralogy and microbiology. N fertilisation significantly reduced MAOC content by 16.0%. The loss of MAOC was primarily attributed to soil acidification (pH decreased from 6.4 to 4.2), leading to exchangeable calcium (Ca) leaching and loss of Ca-bound organic carbon by 37.9% on average. Furthermore, N-induced shifts in dominant microbial keystone taxa from K-strategists (e.g., Actinobacteriota and Sordariomycetes) to r-strategists (e.g., Subgroups 4 and 6 Acidobacteriota) impeded the formation of MAOC through the reduction of microbial carbon use efficiency and oxidase activity (e.g., phenol oxidases and peroxidases). These results suggest that keystone taxa play crucial roles in regulating carbon metabolism and are responsible for MAOC reduction. Moreover, our data pinpoint the importance of Ca leaching for SOC destabilisation, particularly in near-neutral and neutral soils. Full article
(This article belongs to the Section Soil and Plant Nutrition)
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