Search Results (680)

Silicon (Si) accumulation is a widespread anti-herbivore defence in grasses, yet little is known about how insects counteract silicification, including via compensatory feeding, or whether Si-mediated changes in plant stoichiometry also influence herbivore performance. We examined how Si supplementation alters foliar Si, carbon (C), nitrogen (N), and phosphorus (P) in two grasses with contrasting accumulation strategies, Brachypodium distachyon (high accumulator) and Lolium arundinaceum (moderate accumulator), and the consequences for growth and feeding by Helicoverpa armigera. Plants were grown hydroponically with or without Si, and herbivore relative growth rate (RGR), relative consumption (RC), and Efficiency of Conversion of Ingested food (ECI) were measured. Si supplementation had stronger effects on herbivore performance in B. distachyon compared with L. arundinaceum. RGR declined by 126% on B. distachyon compared with 40% on L. arundinaceum. Herbivores increased RC on Si-supplemented L. arundinaceum, with RC positively correlated with foliar Si concentrations, but no compensatory feeding occurred on B. distachyon. N and P concentrations were positively correlated with RGR in L. arundinaceum and ECI in B. distachyon. In conclusion, the degree of Si accumulation in grasses influences both plant stoichiometry and has contrasting impacts on herbivore feeding strategies. Full article

The extensive production and use of per- and polyfluoroalkyl substances (PFAS) over recent decades have resulted in their pervasive distribution in environmental compartments worldwide. PFAS concentrations in soil and biota near fluorochemical manufacturing facilities tend to be typically higher near hotspots, which suggests that the consumption of home-produced foods near such hotspots most likely results in higher human exposure. One prominent European hotspot is located near the 3M fluorochemical production facility in Zwijndrecht (Belgium), where the relative contributions of different exposure pathways remain insufficiently characterised. This study therefore aimed to assess the PFAS concentrations and compositional profiles in serum, dwellings and gardens of teenagers residing near this hotspot. Serum samples from teenagers, along with multiple environmental matrices (i.e., soil, compost, vegetables/fruits/nuts, chicken eggs, rainwater and indoor house dust) were analysed for 21 selected PFAS. Additionally, potential determinants of PFAS occurrence and distribution across matrices were investigated using detailed questionnaire data. We found perfluorooctane sulfonic acid (PFOS) to be the predominant compound in both soil and serum, while perfluorobutanoic acid (PFBA) was most dominant in rainwater, compost, house dust and pods. Perfluorobutane sulfonic acid (PFBS) was most abundant in fruits and chicken eggs, while perfluorododecanoic acid (PFDoDA) was predominant in rooting vegetables and nuts. N-methylperfluorooctane sulfonamidoacetic acid (MePFOSAA) was the dominant compound in fruiting, stem, and leafy vegetables. These results indicate differences in accumulation pathways among the different media and/or differences in affinities of different PFAS in the matrices. Additionally, several environmental and behavioural factors were identified as determinants for PFAS in soil, compost, tree fruits, fruiting vegetables, chicken eggs and house dust, providing insight into potential drivers of exposure variability. The most important factors were related to the soil characteristics, the composting of grass and weeds, the chicken feed (i.e., bread, commercial feed), the type and frequency of ventilation and the frequency of cleaning. Full article

Smart feedlots are increasingly adopting Precision Livestock Farming technologies to enable continuous, individual-animal monitoring and more proactive management in intensive beef production systems. This narrative review synthesises evidence from approximately 350 academic publications, of which 117 are formally cited, complemented by industry deployments and the authors’ experience in smart feedlot system development. We cover enabling digital infrastructure (power, sensing networks, wireless connectivity, and gateways), animal identification and sensing (RFID, automated weighing, wearables, and pen-side sensors), machine vision (RGB, thermal, and multispectral imaging from fixed and mobile platforms), and AI-based analytics and decision support for health, welfare, performance, and environmental management. Across the literature, key components have progressed beyond proof-of-concept toward operation under commercial constraints. Reported outcomes include reduced reliance on routine pen-rider observation and yard handling, earlier triage of emerging morbidity risk and behavioural change, and more standardised welfare auditing. Vision-based methods are repeatedly validated against trained human scorers in both on-farm and abattoir contexts, while automated weighing and image-based liveweight estimation support higher-frequency growth monitoring with low single-digit percentage error in representative studies. Precision feeding and targeted supplementation are associated with improved feed utilisation and reduced resource wastage, although effectiveness and adoption vary across animal classes and production stages. We identify priorities for robust, scalable deployment: resilient communications in harsh environments, appropriate edge–cloud partitioning under intermittent connectivity, and interoperable multi-sensor data fusion to deliver trustworthy alerts and actionable insights. Persistent barriers remain cost, durability, maintenance burden, integration and interoperability, data governance, and workforce capability. Full article

Maternal nutrition during late gestation is critical for fetal development, neonatal resilience, and postnatal adaptation in beef cattle. This study aimed to evaluate the effects of nutritional restriction and supplementation of hydroxytyrosol (HT) in late pregnancy on behavioural, circadian, stress-related, and inflammatory responses in cows and their restricted nursed offspring. Pregnant cows were allocated to a 2 × 2 factorial experimental design (feeding level: T100% vs. T60% of nutrient requirements; HT: 0 vs. 180 mg/kg of diet). Cow behaviours were recorded during meals (from week −12 prepartum to term), and calf activities, body temperature, and mother–offspring interactions were assessed at 5 weeks postpartum. Nutritional restriction accelerated feed intake in cows and increased stress-related behaviours, while HT partially mitigated these effects. Molecular analyses in blood samples revealed dynamic prepartum upregulation of glucocorticoid-receptor NR3C1 in week −6, and downregulation of circadian (BMAL1, PER1, CRY1) gene expression in week 5 after parturition, both in T60%-HT cows. In calves, maternal HT supplementation promoted active exploratory behaviour, and counteracted behavioural and circadian (CRY1 and PER1) and inflammatory markers (IL8) gene expression resulting from prenatal nutrient restriction, leading to behavioural profiles and blood gene expression comparable to those observed in calves born to adequately fed dams. Full article

Background: Undernutrition among children under five years remains a major public health challenge, particularly in low- and middle-income countries and rural communities where poverty, food insecurity, and limited access to health services persist. Maternal and caregiver perceptions play a critical role in shaping feeding practices and health-seeking behaviours that influence child nutritional outcomes. Objective: This study explored mothers’ and caregivers’ perceptions of factors contributing to undernutrition among children under five years in a rural community of Ngqeleni, Eastern Cape, South Africa. Methods: A qualitative descriptive study was conducted at a primary healthcare clinic in the Ngqeleni sub-district. Purposive sampling was used to recruit mothers and caregivers of children under five years. Data were collected through seven in-depth interviews and three focus group discussions involving a total of 25 participants. Interviews were conducted using a semi-structured guide and analyzed thematically. Results: Five major themes emerged: caregivers’ perceptions of nutrition, household food insecurity and unemployment, limited dietary diversity, culturally influenced feeding practices, and gaps in practical nutrition knowledge. Caregivers demonstrated concern for child nutrition but described constrained feeding choices shaped by poverty, reliance on social grants, environmental challenges, and limited access to diverse foods. Environmental challenges such as drought and lack of piped water further limited food production. Limited nutrition knowledge and reliance on informal information sources contributed to suboptimal feeding practices. Conclusions: Undernutrition in this rural setting is shaped by a complex interaction of economic hardship, environmental constraints, and limited caregiver knowledge. Community-based nutrition education, strengthened primary healthcare counselling, and multisectoral interventions addressing poverty, water access, and food security are essential to improve child nutrition outcomes. Full article

The objective of this article is to optimise the deposition modes and the content of exothermic additions (EAs) in the core filler in Fe-C-Cr-Ti with Cu additions hardfacing. To achieve this, JMatPro Release 7.0, Sente Software Ltd., 2016 material characterisation software was used to simulate and calculate the equilibrium phase structure and composition of the Fe-C-Cr-Ti-Cu alloy during the welding thermal cycle. A synergistic approach combining the Taguchi–Analysis of Variance (ANOVA)–Factorial design (FD) method with the standard hybrid Taguchi–ANOVA–Principal Component Analysis (PCA)–Grey Relational Analysis (GRA) is used and justified to optimise factors and develop mathematical models for parameters in the L9 orthogonal experimental design. The study examines how the transfers of deoxidisers depend on the content of exothermic additions in the cored wire filler (EA) and the contact tip-to-work distance (CTWD), while the behaviour of carbide formers is influenced by wire feed speed (WFS) and present arc voltage at the power source (U_set). The research specifically investigates the Fe-C-Cr-Ti-Cu system and the role of copper in stabilising austenite. Findings show that high Cu concentrations (7 wt.%) enhance hardenability by 13%, effectively suppressing pearlite transformation and expanding the bainite region. The desired chemical composition of the deposited metal is determined by the distribution of selected factors, as measured by the transfer coefficients of each element. Full article

The sense of smell is the first sense by which horses determine the suitability of feed for consumption. The aim of the study was to assess the influence of the degree of familiarity with the smell and taste of plants on horses’ olfactory exploration. Behavioural responses of twenty adult horses to nine herbs of three degrees of familiarity—least familiar (unknown in the first trial), known only by smell, and known by smell and taste—were investigated. During the tests, the horses were allowed to explore the individual herbs placed in a crib constructed to prevent them from eating the contents. Horses’ pre-consuming behaviour towards olfactory cues of plants was mainly expressed by different times of exploration. The horses explored herbs known by smell and taste less intensively than those initially unfamiliar, but not less intensively than herbs known only by smell. Thus, having more sensory experience (taste and olfactory) with a plant in the past, horses are often quicker to recognise plants based solely on the sense of smell in the future. The sex and type of the horse (warmblood, pony) may influence responses towards herbs of different levels of familiarity. Full article

Ecotourism in protected areas creates a conservation paradox: tourism revenue funds protection, yet tourism infrastructure simultaneously degrades the wildlife it protects. We examined this paradox in white-faced capuchins (Cebus imitator) in Tortuguero National Park, comparing behaviour across a high-tourism accommodation site (2152 monthly guests) and a strictly regulated terrestrial trail. Using focal animal and sweep sampling methods, we recorded 477 behavioural units across 261 min, analysing locomotion, feeding, and agonistic behaviours through generalized linear models. Primates in accommodation areas exhibited significantly reduced high substrate use (p = 0.005), showed a trend toward increased anthropogenic food reliance (p = 0.070), and higher—but not statistically significant—rates of agonistic behaviours (p > 0.05). The negative correlation between natural foraging and active food supply (r = −0.31) is consistent with anthropogenic provisioning that may alter primate ecological functions. These findings demonstrate that effective conservation in tourism contexts requires integrated management addressing three interconnected challenges: (1) habituation to human presence, (2) food provisioning with cascading consequences, and (3) ecosystem-level degradation through altered primate functions. We recommend evidence-based interventions including secured waste management, enforcement of wildlife feeding prohibitions, and environmental education programs with community participation. Ecotourism sustainability requires managing human–wildlife interactions and integrating local stakeholder perspectives to preserve animal welfare and ecosystem functions essential for conservation. Full article

Goat (Capra hircus) welfare is an important issue in any farming system. The aim of the study was a comprehensive analysis of the impact of environmental factors and farming practices on the welfare of goats, with particular attention to physical, behavioural, and emotional aspects. It includes a review of the up-to-date literature on the effects of environmental conditions including air temperature, air humidity, space, feeding systems, social relationships (mother–offspring, human–animal, animal–animal), zootechnical procedures (dehorning, castration, hoof trimming) and welfare assessment methods. It compares the AWIN, Anzuino, Muri and Leite protocols for assessing goat welfare and their application in the Five Domain Model. Goat welfare is strongly influenced by their environment, nutrition and socialisation: heat stress and confined space cause physiological disorders, decreased immunity and increased aggressive behaviour and a monotonous diet leads to frustration and reduced cognitive activity, whereas positive early contact with humans reduces anxiety and maintaining the mother–kid bond supports the social development of young goats. Furthermore, significant improvements in welfare and stress reduction can be achieved by providing anaesthesia and painkillers where necessary to minimise pain and enriching the environment with items that support natural behaviour, such as platforms, brushes and items for cognitive tasks. In general, the keeper should take a holistic approach, combining environmental optimisation, humane husbandry practices and regular monitoring using validated assessment protocols to improve welfare. These measures are both an ethical obligation and a prerequisite for animal health and production efficiency. Nevertheless, there is a need for further research focussing on the development of non-invasive assessment methods and innovative forms of environmental enrichment. Full article

The European green crab (Carcinus maenas) is an intertidal crustacean that has extended its invasive range globally and is regarded as a major pest species for bivalves, impacting coastal food chains and aquacultures. Crabs primarily use chemosensory cues to sense their environment for feeding to avoid predation and to locate mates and induce mating. Consequently, known attractants including food baits are frequently employed in trapping and control efforts. Here, we investigate the effects of introducing a predatory fish odour to the environment and show that it elicits anti-predator behaviour in C. maenas. A 45% reduction in crab foraging and feeding behaviour was observed when predator odour was introduced compared with food control experiments. A further 23% reduction (68% reduction overall) in feeding behaviour was observed after crabs were exposed to (though housed separately from) the same seawater as the live predator for several days. This highlights the increased awareness or continuous stress of these habituated crabs towards predator odour. This study also presents potential for application as a deterrent in integrated pest management strategies for this global invader. Full article

Non-recyclable plastic waste (PSW) and residual lignocellulosic biomass (WP) represent abundant yet underused resources whose conversion can generate renewable fuels with synergistic benefits. While conventional pyrolysis remains limited by slow heat transfer and poor adaptability to heterogeneous feeds, microwave-assisted pyrolysis (MAP) offers faster volumetric heating and improved syngas quality, though it is still largely confined to the laboratory scale due to limited understanding of feedstock interactions and process behaviour. In this context, the present work provides a laboratory-scale experimental investigation of the MAP co-pyrolysis of PSW/WP blends, focusing on gas yield and syngas quality, and complements the experimental analysis with a preliminary scale-up assessment for a continuous microwave reactor. The results reveal clear synergistic effects, with gas yields exceeding those predicted by linear mixing. A 70/30 wt% PSW/WP blend produced a hydrogen-rich syngas with H₂ concentrations of approximately 42 vol% and an H₂/CO ratio of 2–3. Compared to conventional pyrolysis under analogous conditions, MAP increased hydrogen content by around 35% and reduced CO₂ concentrations by up to 40%, resulting in a cleaner and more energy-dense gas. Overall, the findings highlight the strong potential of MAP for the valorization of mixed plastic–biomass wastes. Full article

Changes in horse management and use mean that a growing number of horses are now kept into older age, while age-specific management practices for these animals remain insufficiently documented. This cross-sectional online survey aimed to describe the housing, feeding, activity, behaviour and care routines of horses aged 20 years and older in Germany, based on 923 questionnaires completed by horse owners about their senior horses. Most horses maintained regular tactile social contact (87.4%, n = 805), and over half were still ridden or driven (61.6%, n = 566). However, the proportion of horses in active riding or driving use declined strongly with age, from 78% in 20–24-year-old horses to 57% in those aged 25–29 years and 34% in horses aged 30 years or older (p < 0.0001). Daily grooming likewise decreased with age, falling from 58% in the youngest age group to 40% in the oldest, while weekly grooming became more common (20–24 vs. ≥30 years: p < 0.0001; 25–29 vs. ≥30 years: p = 0.0017). Behavioural patterns were largely stable, although the proportion of horses reported to have lost social rank within the herd increased from 25% in 20–24-year-old horses to 41% in horses aged 30 years or older (20–24 vs. 25–29 years: p = 0.0050; 20–24 vs. ≥30 years: p = 0.0002). Feeding practices also showed pronounced age-related differences: the use of long-fibered dry roughage declined from 90% in the youngest group to 69% in the oldest, whereas the use of pre-chopped dry roughage increased from 21% to 73% (both p < 0.0001). The provision of mineral and vitamin supplements likewise decreased with age, from 51% of horses in the youngest group to 35% in the oldest (p = 0.0026). These findings indicate consistent differences between age groups in owner-reported management practices and highlight the importance of considering age when evaluating how older horses are managed. Full article

Mediterranean monk seal (Monachus monachus) sightings along the Lebanese coast were recorded between 2020 and 2025. This study aims to provide insights into the consistency of monk seal presence, their habitat use, and their feeding behaviour in the studied area. The research relied on photographic and video materials gathered from social media reports, as well as contributions from local fishers and divers. A total of 43 sightings were recorded, with photo-identification possible for 34 of these. The study confirmed the presence of at least three distinct individuals, including one adult female frequently utilizing marine caves in Amchit and Rawsheh. Video recordings of feeding behaviour revealed prey species such as grey mullet and octopus, which are also targeted by local fisheries, suggesting potential conflicts between the seals and the fishing sector. This study emphasizes the need for a more systematic, long-term monitoring approach, including the use of infrared cameras, to identify suitable habitats and more accurately assess seal presence. The research further recommends a region-wide effort to understand monk seal movements within the Levantine Basin and to support broader conservation initiatives for the species. Full article

Fishes contribute to the biological carbon pump, yet their overall role remains poorly constrained due to the difficulty of obtaining direct metabolic measurements and, consequently, is poorly understood. Electron transport system (ETS) activity is commonly used as a proxy for potential respiration, but its application requires an appropriate relationship between respiration (R, measured as oxygen consumption MO₂) and ETS activity. Here, we examined the relationship between swimming activity, oxygen consumption, and ETS activity in juvenile Sparus aurata using swimming-tunnel respirometry. Oxygen consumption increased with swimming speed following a four-parameter sigmoidal model, whereas ETS activity remained independent of short-term changes in activity. Normalizing respiration by ETS produced R/ETS ratios ranging from 0.17 to 0.71, values consistent with those reported for zooplankton and micronekton. Lower ratios correspond to minimal aerobic demand and may represent quiescent behaviour, while higher ratios reflect elevated demands associated with active movement or feeding. These ratios are suggested for the assessment of respiration rates from ETS activity during diel vertical migration in the ocean to improve estimates of respiratory flux. However, methodological issues related to ETS activity in different body regions must be solved to enable reliable measurements. Full article

Avocado (Persea americana Mill) is a crop of significant economic importance in Ecuador, facing major threats from insect pests such as Monalonion velezangeli (Hemiptera: Miridae), which can reduce production by up to 42%. This study evaluated the effectiveness of six treatments based on physical barriers and biorational products within an integrated pest management (IPM) framework in an experimental avocado orchard (cv. Fuerte) in Pichincha, Ecuador. The treatments included pyrethroid, kaolin, mineral oil, combinations of kaolin and mineral oil, fruit bagging and a water control. The Kruskal–Wallis analysis revealed significant differences among treatments (T = 23.10, df = 6, p = 0.000765). Fruit bagging and a pyrethroid emerged as the most effective strategies, with mean pest damage incidences of 10.33 and 9.17%, respectively, while the control treatment showed the highest pest damage incidence (32.83%). The combination of mineral oil and kaolin demonstrated intermediate efficacy, acting as a physical barrier that interferes with the pest’s feeding and oviposition behaviours. Although fruit bagging proved highly effective, its adoption in commercial systems is limited by cost and time requirements. Full article