Search Results (949)

This study investigates the production and economic feasibility of biochar for smallholder and family farms in Central Amazonia, with potential implications for other tropical regions. The costs of construction of a prototype mobile kiln and biochar production were evaluated, using small-sized biomass from acai (Euterpe oleracea Mart.) agro-industrial residues as feedstock. The biochar produced was characterised in terms of its liming capacity (calcium carbonate equivalence, CaCO_3eq), nutrient content via organic fertilisation methods, and ash analysis by ICP-OES. Field trials with cowpea assessed economic outcomes, as well scenarios of fractional biochar application and cost comparison between biochar production in the prototype kiln and a traditional earth-brick kiln. The prototype kiln showed production costs of USD 0.87–2.06 kg⁻¹, whereas traditional kiln significantly reduced costs (USD 0.03–0.08 kg⁻¹). Biochar application alone increased cowpea revenue by 34%, while combining biochar and lime raised cowpea revenues by up to 84.6%. Owing to high input costs and the low value of the crop, the control treatment generated greater net revenue compared to treatments using lime alone. Moreover, biochar produced in traditional kilns provided a 94% increase in net revenue compared to liming. The estimated externalities indicated that carbon credits represented the most significant potential source of income (USD 2217 ha⁻¹). Finally, fractional biochar application in ten years can retain over 97% of soil carbon content, demonstrating potential for sustainable agriculture and carbon sequestration and a potential further motivation for farmers if integrated into carbon markets. Public policies and technological adaptations are essential for facilitating biochar adoption by small-scale tropical farmers. Full article

The scarab species Protaetia brevitarsis, an edible insect, has been used in traditional medicine, as animal feed, and for converting agricultural organic wastes into biofertilizer. The intestinal tract, which contains a diverse array of microbiota, including viruses, plays a critical role in animal health and homeostasis. We previously conducted a comparative analysis of the gut microbiota of third-instar larvae of P. brevitarsis obtained from five different farms and found significant differences in the composition of the gut bacterial microbiota between farms. To better understand the gut microbiota, the composition of DNA viruses in the hindgut contents of P. brevitarsis larvae obtained from five farms was investigated using metagenomic sequencing in this study. The β-diversity was significantly different between metagenomic data obtained from the five farms (PERMANOVA, pseudo-F = 46.95, p = 0.002). Family-based taxonomic analysis indicated that the relative abundance of viruses in the gut overall metagenome varied significantly between farms, with viral reads comprising approximately 41.2%, 15.0%, 4.3%, 4.0%, and 1.6% of metagenomic sequences from the farms Tohamsan gumbengi farm (TO), Secomnalagum gumbengi (IS), Gumbengi brothers (BR), Kyungpook farm (KB), and Jhbio (JH), respectively. More than 98% of the DNA viruses in the hindgut were bacteriophages, mainly belonging to the Siphoviridae family. At the species level, Phage Min1, infecting the genus Microbacterium, was detected in all farms, and it was the most abundant bacteriophage in intestinal microbiota, with a prevalence of 0.9% to 29.09%. The detected eukaryotic DNA viruses accounted for 0.01% to 0.06% of the intestinal microbiota and showed little or no relationship with insect viruses. Therefore, they most likely originated from contaminated feed or soil. These results suggest that the condition of substrates used as feed is more important than genetic factors in shaping the intestinal viral microbiota of P. brevitarsis larvae. These results can be used as reference data for understanding the hindgut microbiota of P. brevitarsis larvae and, more generally, the gut virome of insects. Full article

This paper examines the farming systems and operational structures in the Region of Western Macedonia (RWM), Greece and constructs a typology of farms based on structural, operational, and socio-economic characteristics. Agriculture remains a vital pillar of the regional economy, particularly in the context of RWM’s ongoing transition to a post-lignite development model. Using farm-level data from the 2018 Farm Accountancy Data Network (FADN), Principal Component Analysis (PCA) identified four latent dimensions of farm heterogeneity—income and productivity, asset base, land size, and labour structure. Hierarchical and K-means cluster analysis revealed three distinct farm types: (1) medium-sized, high-efficiency farms with moderate reliance on subsidies (30% of the sample); (2) small-scale, family farms with modest productivity and limited capitalisation (48%); and (3) large, asset-rich farms exhibiting structural inefficiencies and lower output per hectare (22%). These findings highlight structural vulnerabilities, particularly the predominance of undercapitalised smallholdings, and provide a data-driven foundation for Thdesigning differentiated policies that support farm resilience, generational renewal, and sustainable rural development. Full article

Farming is a uniquely demanding occupation, with family and work often deeply intertwined. Whilst this integration is common amongst the agricultural sector in Ireland (99.7% farms classified as family), it can lead to a conflict of responsibilities, but this has not been examined to date. Therefore, this study aimed to examine work–family conflict (WFC) in farmers in Ireland, and if WFC differs based on socio-demographic factors, mental health, social support and farm-specific stressors. A cross-sectional study examined WFC in 446 farmers in Ireland. Depression, anxiety, stress, suicidality, general mental health, psychological flexibility, cognitive fusion, self-experience, perceived social support, farm stress and attachment were also measured. Participants reported moderately high levels of WFC (25.1 ± 7.2). Being younger, male, having children aged 5 years or less, higher reported working hours on the farm, higher farm-specific stressors, and lower psychological flexibility contributed to higher WFC. In addition, lower social support, mental health and psychological skills were significantly associated with higher WFC. Future research should consider the integrated nature of work and family in farm life and use a qualitative approach to further understand gendered experiences of WFC. Full article

Small-scale fisheries and aquaculture play a crucial role in securing food, income, and nutrition for millions, especially in the Global South. Rural small-scale aquaculture (SSA) is characterized by limited investment and technical training among farmers, diversification and dispersion of farms over large areas, reduced access to competitive markets for inputs and products, and family labor. Small-scale integrated circular aquaponic (ICAq) systems, in which systems’ component outputs are transformed into component inputs, have significant potential to increase circularity and promote economic development, especially in a rural context. We offer an integrated and comprehensive approach centered on aquaponics or aquaponic farming for small-scale aquaculture units. It aims to identify and describe a series of circular processes and causal links that can be implemented based on deep study in SSA and ICAq. Circular processes to treat by-products in ICAq include components like composting, vermicomposting, aerobic and anaerobic digestion, silage, and insect production. These processes can produce ICAq inputs such as seedling substrates, plant fertilizers, bioenergy, or feed ingredients. In addition, the plant component can supply therapeutic compounds. Further research on characterization of aquaponic components outputs and its quantifications, the impact of using circular inputs generated within the ICAq, and the technical feasibility and economic viability of circular processes in the context of SSA is needed. Full article

Background/Objectives: This study aimed to explore how stressors and facilitators within the work–family interface (WFI) influence mental health outcomes among farming women in rural Chile. The research sought to identify key relational patterns and contextual determinants shaping psychological well-being in this population. Methods: An exploratory mixed-methods design was employed, involving 41 semi-structured interviews analyzed using grounded theory. Qualitative themes were quantified by calculating the percentage of occurrence per interview, allowing for comparative analysis. Pearson correlation and principal component analysis (PCA) were used to examine associations among WFI dimensions and mental health-related variables. Results: Strong and statistically significant correlations emerged between institutional and community facilitators (r = 0.664, p < 0.01) and between gender facilitators and family workload stressors (r = 0.609, p < 0.01). PCA revealed two distinct patterns: women who rely on institutional support often resist traditional family roles, while others find balance through familial support systems. The gendered distribution of caregiving and productive tasks was a key factor in psychological well-being, with some women reporting physical discomfort linked to triple workloads. Conclusions: The dynamics of the WFI in rural contexts are shaped by both sociocultural and institutional factors. The findings highlight the need for culturally sensitive mental health policies that acknowledge and respond to the lived experiences of farming women. Full article

In farmed animals, body color is not only an ecological trait but also an important trait that influences the commercial value of the animals. Melanin plays an important role in the formation of body color in animals, while the tyrosinase (TYR) gene family is a group of key enzymes that regulate melanogenesis. The Chinese soft-shelled turtle (Pelodiscus sinensis) is one of the most important reptiles in freshwater aquaculture. However, the potential role of the TYR gene family in the body color formation of P. sinensis remains unclear. This study aimed to investigate the expression and conservation of the TYR gene family in relation to body color variation in P. sinensis. Three core members of this gene family were identified from the P. sinensis genome. Following identification, the genomic features were analyzed. They shared similar physicochemical properties and conserved domains. Chromosome mapping showed that the three genes of P. sinensis were all located on the autosomes, while phylogenetic and collinearity analysis suggested that the protein functions of the three genes in the studied species were highly conserved. Amino acid sequence alignment indicated high conservation among the three TYR gene family proteins (TYR, TYRP1, and DCT) in multiple critical regions, particularly in their hydrophobic N-/C-terminal regions and cysteine/histidine-rich conserved domains. The qRT-PCR revealed that the TYR and DCT genes were highly expressed in the eyes of individuals with different body colors. The expression levels of TYR and TYRP1 genes in the skin were significantly higher in dark-colored individuals than in light-colored ones (p < 0.05). These results will lay the groundwork for functional studies and breeding programs targeting color traits in aquaculture. Full article

This study examines Poland’s agricultural land market between 2009 and 2023 through fixed effects and spatial econometric models, highlighting economic and spatial determinants of land prices. Key results show that GDP per capita strongly increases land values (β = +0.699, p < 0.001), while agricultural gross value added (–2.698, p = 0.009), soil quality (–6.241, p < 0.001), and land turnover (–0.395, p < 0.001) are associated with lower prices. Spatial dependence is confirmed (λ = 0.74), revealing strong regional spillovers. The volume of state-owned WRSP land sales declined from 37.4 thousand hectares in 2015 to 3.1 thousand hectares in 2023, while non-market transfers, such as donations, exceeded 49,000 annually. Although these trends support farmland protection and family farms, they also reduce market mobility and hinder generational renewal. The findings call for more flexible, sustainability-oriented land governance that combines ecological performance, regional equity, and improved access for young farmers. Full article

Background: Food environments can determine food choices, acting as barriers to or promoters of healthy eating. It is necessary to investigate individuals’ perceptions of those barriers and promoters of healthy eating in the food environment. Methods: This is a qualitative and quantitative study involving patients diagnosed with arterial hypertension. In the quantitative approach, a validated questionnaire for the Brazilian population, the Perceived Nutrition Environment Measures Survey, was used. For the qualitative approach, a talking map was applied in a focus group with guiding questions. Quantitative data were analyzed through simple relative frequency, and qualitative data through reports; subsequently, both were grouped into perceived barriers and facilitators. Results: Participants found high access to ultra-processed foods, strongly influenced by advertising in commercial establishments, as a barrier, as well as barriers related to changes in commensality habits and transformations in food systems. As promoting factors, access to fruits and vegetables was highlighted as favoring healthier food choices. The qualitative findings emphasized the importance of home gardens and foods sourced from family farming. Conclusions: This study found that individuals perceive high access to ultra-processed foods in their food environment, both in financial terms and availability, while reporting low access to fresh foods. Full article

The Ghris watershed in southern Morocco is a significant ecological and agricultural area. However, due to the current impacts of climate change, farming activities, and pollution, data on its quality and biological importance need to be updated. Therefore, this study aimed to evaluate the physico-chemical and biological quality of surface water in the Ghris River. The Water Quality Index (WQI) and the Iberian Biological Monitoring Working Group (IBMWP) index were used to assess water quality along four sampling sites in 2024. The collected data were analyzed with descriptive and multivariate statistics. In total, 424 benthic macroinvertebrates belonging to seven orders were identified in the surface waters of the Ghris basin. These microfauna were significantly variable among the studied sites (p < 0.05). Station S4 is significantly rich in species, including seven orders and nine families of macroinvertebrates, followed by Station S2, with seven orders and eight families. Stations S3 and S1 showed less species diversity, with three orders and one family, respectively. The Insecta comprised 95.9% of the abundance, while the Crustacea constituted just 4.1%. The physico-chemical parameters significantly surpassed (p < 0.05) the specified norms of surface water in Morocco. This indicates a decline in the water quality of the studied sites. The findings of the principal component analysis (PCA) demonstrate that the top two axes explain 87% of the cumulative variation in the data. Stations 2 and 3 are closely associated with high concentrations of pollutants, notably Cl⁻, SO₄²⁻, NO₃⁻, and K⁺ ions. Dissolved oxygen (DO) showed a slight correlation with S2 and S3, while S4 was characterized by high COD and PO₄ concentrations, low levels of mineral components (except Cl⁻), and average temperature conditions. Bioindication scores for macroinvertebrate groups ranging from 1 to 10 enabled the assessment of pollution’s influence on aquatic biodiversity. The IBMWP biotic index indicated discrepancies in water quality across the sites. This study gives the first insight and updated data on the biological and chemical quality of surface water in the Ghris River and the entire aquatic ecosystem in southeast Morocco. These data are proposed as a reference for North African and Southern European rivers. However, more investigations are needed to evaluate the impacts of farming, mining, and urbanization on the surface and ground waters in the study zone. Similarly, it is vital to carry out additional research in arid and semi-arid zones since there is a paucity of understanding regarding taxonomic and functional diversity, as well as the physico-chemical factors impacting water quality. Full article

In the context of increasing demands for sustainable natural resource management, this study analyzes the demographic and structural characteristics of family farms in Croatia and their participation in three environmental measures under the 2014–2022 Rural Development Programme. Using national administrative data and spatial analysis, the results highlight key patterns linked to age, education, gender, and ownership, as well as regional disparities in measure uptake. The findings suggest the importance of tailoring rural development policy to small farms’ specific needs to improve future CAP implementation. Full article

Lavandula angustifolia Mill., lavender, is an aromatic plant in the Lamiaceae family. Lavender, which is native to the Mediterranean region but cultivated throughout the world, is an important economic plant. Several studies have investigated two aspects of this aromatic plant: (1) which pollinators, particularly bees, pollinate lavender, and (2) the composition of lavender essential oil. However, little research has been conducted to investigate how pollination affects either the yield or phytochemistry of lavender. The current study, which was conducted in North America, investigates which bee species visit lavender and how pollination affects plant chemistry, specifically the essential oil produced by lavender. Over the course of the 5-week observational period, a total of 12 species (across 10 genera) of bees were identified visiting lavender. Compared to previous studies on cultivated lavender at the same site (Mt. Nebo Botanical Farm, Mona, UT), four bee species not previously observed on lavender were identified. These included Hoplitis producta, Nomada sp., Osmia trevoris, and Megachile snowi. Pollinated lavender, compared to lavender excluded from pollinators, produced more essential oil (yield (w/w) = 1.49% vs. 1.07%), lower relative amounts of linalool (35.4% vs. 39.9%), and higher relative amounts of linalyl acetate (21.3% vs. 16.8%). The findings of this study demonstrate the ecological interactions between pollinators and lavender, and how those interactions impact phytochemistry. Full article

Changing climatic conditions are significant determinants of agricultural productivity. Rio Grande do Sul is the southernmost state and the second-largest agricultural producer in Brazil. The suitability of its land for farming can be used as a proxy for agricultural and economic success, making it a pertinent case for exploring the consequences of climate change on major crop production. The latest available climate and environmental data was used to develop an agricultural Suitability Index (SI) that quantifies the suitability of land for rice, tobacco, soybean, and corn production in 2020 (present), 2050 (near-future), and 2100 (far-future) under moderate (SSP245) and extreme (SSP585) climate scenarios. SI scores for each municipality of Rio Grande do Sul consider inputs from a three-layer framework (climatic, non-climatic, and current production) to provide critical insight into potential shifts in agricultural productivity. While terrestrial suitability for crop growth varies both spatially and temporally, widespread decreases in suitability for all four crops are expected across the state under both scenarios. Soybean is expected to be the least affected crop, and rice is the most affected crop, tied to shifting patterns in precipitation, which significantly determines suitability. Local and state governments, agribusinesses, and family producers will have to adapt to environmental challenges to ensure the provision of food, labor, and economic security. Full article

Weeds present a pervasive challenge in agricultural fields. The integration of herbicide-resistant crops with chemical weed management offers an effective solution for sustainable weed control while reducing labor inputs, particularly in large-scale intensive farming systems. Consequently, the development of herbicide-resistant cultivars has emerged as an urgent priority. In this study, we found that the G311E mutant gene of Arabidopsis MATE (multidrug and toxic compound extrusion) family transporter DTX6, designated DTX6m, confers robust resistance to bipyridyl herbicides paraquat and diquat in rice. DTX6m-overexpression lines exhibited marked resistance to these two herbicides, tolerating diquat concentrations up to 5 g/L, which is five-fold higher than the recommended field application dosage. Agronomic assessments demonstrated that grain yields of DTX6m-overexpressing plants were statistically equivalent to those of wild-type plants. Moreover, the plants displayed beneficial phenotypic changes, such as accelerated flowering and a slight reduction in height. Seed morphometric analysis indicated that in comparison with the wild-type control, DTX6m-transgenic lines exhibited altered grain dimensions while maintaining consistent 1000-grain weight. Nutritional assays further demonstrated that DTX6m increased the levels of free amino acids in seeds, while normal protein and starch contents were retained. Collectively, these results establish that DTX6m effectively boosts rice resistance to paraquat and diquat, validating DTX6m as a candidate gene for engineering plant herbicide resistance and also implying a potential role for DTX6m in amino acid homeostasis in plants. Full article

Encephalitozoon cuniculi is a microsporidian widely spread in rabbits (Oryctolagus cuniculus) and other species, including humans, causative of neurological disorders or remaining in a latent state in the host organism. The aim of this study was to estimate the prevalence of E. cuniculi in rabbits located in the North-Western region of Romania, and to run a comparative diagnosis for E. cuniculi by multiple methods. A total of 381 rabbits were included, originating from households, family farms and wildlife, which were subjected to serological and/or molecular genetics diagnostic methods for E. cuniculi identification. Seropositivity of 43.02% (151/351) was obtained by ELISA, together with a 45.45% (110/242) prevalence from urine, feces and organs by nested PCR. Additionally, a prevalence of 48.39% (15/31) was identified by a comparative real-time PCR (qPCR). The urinary bladder is firstly reported for molecular E. cuniculi diagnosis, with a positivity of 30.56% (11/36) by nested PCR. Despite the disagreement between the diagnostic methods, the present results highlight the level of pathogen dissemination among rabbits in North-Western Romania that represents a risk for not only rabbits and other animals, but also for the general public by its zoonotic character. Full article