Search Results (17)

Detecting manipulated document images is essential for verifying the authenticity of official records and preventing document forgery. However, forgery artifacts are often subtle and localized in fine-grained regions, such as text boundaries or character outlines, where visual symmetry and structural regularity are typically expected. These manipulations can disrupt the inherent symmetry of document layouts, making the detection of such inconsistencies crucial for forgery identification. Conventional CNN-based models face limitations in capturing such edge-level asymmetric features, as edge-related information tends to weaken through repeated convolution and pooling operations. To address this issue, this study proposes an edge-focused method composed of two components: the Edge Attention (EA) layer and the Edge Concatenation (EC) layer. The EA layer dynamically identifies channels that are highly responsive to edge features in the input feature map and applies learnable weights to emphasize them, enhancing the representation of boundary-related information, thereby emphasizing structurally significant boundaries. Subsequently, the EC layer extracts edge maps from the input image using the Sobel filter and concatenates them with the original feature maps along the channel dimension, allowing the model to explicitly incorporate edge information. To evaluate the effectiveness and compatibility of the proposed method, it was initially applied to a simple CNN architecture to isolate its impact. Subsequently, it was integrated into various widely used models, including DenseNet121, ResNet50, Vision Transformer (ViT), and a CAE-SVM-based document forgery detection model. Experiments were conducted on the DocTamper, Receipt, and MIDV-2020 datasets to assess classification accuracy and F1-score using both original and forged text images. Across all model architectures and datasets, the proposed EA–EC method consistently improved model performance, particularly by increasing sensitivity to asymmetric manipulations around text boundaries. These results demonstrate that the proposed edge-focused approach is not only effective but also highly adaptable, serving as a lightweight and modular extension that can be easily incorporated into existing deep learning-based document forgery detection frameworks. By reinforcing attention to structural inconsistencies often missed by standard convolutional networks, the proposed method provides a practical solution for enhancing the robustness and generalizability of forgery detection systems. Full article

Glioblastoma (GBM), a highly malignant brain tumor, arises within a complex microenvironment that plays a critical role in facilitating tumor progression, ensuring survival, and enabling immune evasion, ultimately contributing to therapeutic resistance. Cancer-associated fibrosis is increasingly recognized as a key factor in the tumor pathophysiology, particularly in extracranial cancers, and reported therapeutic strategies in several cancers consist of the current use of the standard-of-care treatment combined with anti-fibrotic drugs. However, it remains unclear how the fibrotic changes associated with the GBM microenvironment contribute to the transformation of GBM from a chemosensitive state to a chemoresistant one. Here, we developed an in vitro model that mimics a fibrosis-like mechanism using the U-87MG GBM cell line. To achieve this, we identified the optimal experimental conditions (i.e., U-87MG cultured in serum-deprivation medium in the presence of recombinant TGF-B1 at 5 ng/mL for 72 h) that effectively induced fibrosis, as suggested by the counter-regulated expression of E- and N-cadherin and sustained levels of α-SMA and collagen I. As expected, U-87MG fibrotic cells were demonstrated to be more resistant to TMZ (predicted EC₅₀ = 35 µM) as compared to the non-fibrotic counterpart (EC₅₀ not achieved here; predicted EC₅₀ = 351 µM). Accordingly, the anti-fibrotic uPAcyclin—a new derivative cyclic compound inspired as a A6 decapeptide drug—showed a significant cytotoxic effect, sensitizing resistant U-87MG fibrotic cells to TMZ. This highlights that targeting fibrosis may help to overcome TMZ resistance in GBM. Full article

Waterholes in semi-arid environment are sections of rivers that fill during high river flows or floods and keep water once flow ceases. They are essential water sources for rive ecosystems. Some waterholes remain even during prolonged droughts. The resilience of ecosystems in these environments depends on the persistence of the waterholes. While most semi-arid, ephemeral river systems are disconnected from regional groundwater and losing in most parts there may be some sections that can be connected to localised groundwater or parafluvial areas. To assess the persistence of waterholes the groundwater contribution to the water balance needs to be addressed. This study assesses groundwater connectivity to waterholes in a part of the Murray-Darling Basin, one of the largest watersheds in the world, using environmental tracers radon and stable isotopes. Approximately 100 samples were collected from 27 waterholes along the Narran, Calgoa, Barwon and Darling rivers, as well as 8 groundwater bore samples. The assessment of groundwater connectivity or the lack of is necessary from water balance modelling and estimation of persistence of these waterholes. As expected, the results indicate consistently low radon concentrations in the waterholes and very small deviation in stable isotopes ${\delta }^{18}$O and ${\delta }^2$H. In general, most of these waterholes are losing water to groundwater, indicated by low salinity (EC values) and low radon concentrations. While radon concentrations are small in most cases and indicative of little groundwater contributions, some variability can be assigned to bank return and parafluvial flow. It indicates that these contributions may have implications for waterhole persistence in ephemeral streams. The study demonstrates that in some cases local bank return flow or parafluvial flow may contribute to waterhole persistence. Full article

(1) Background: Our study explores the relationship between expected, perceived, and measured effects of botulinum toxin A (BoNT-A) treatment and saline (placebo) in children and adolescents with cerebral palsy (CP) in the calf muscles of 20 children and adolescents with cerebral palsy (CP), aged 4–15 years, using the Gross Motor Function Classification System (GMFCS) I–II. (2) Methods: A mixed methods parallel database design was used. Quantitative and qualitative data were collected at baseline and four weeks after treatment. The primary quantitative measure was gross energy cost (EC) during walking, obtained from a 5-Minute Walk Test (5MWT), while qualitative semi-structured interviews were performed with each parent and child/adolescent individually. (3) Results: Four weeks after treatment, we did not find any correspondence between expected, measured, and perceived effects. Interestingly, parental perceptions of treatment effects were more consistent than the measured outcomes. We also observed a connection between parental treatment expectations and perceived effects, often related to reduced energy expenditure. Children tended to view their parents as treatment experts and had fewer expectations and perceptions themselves. (4) Conclusions: These findings support the importance of child-centered care, which entails actively listening to children’s expectations and perceptions during the treatment process. Full article

Multiple input multiple output (MIMO) technology necessitates detection methods with high performance and low complexity; however, the detection problem becomes severe when high-order constellations are employed. Variational approximation-based algorithms prove to deal with this problem efficiently, especially for high-order MIMO systems. Two typical algorithms named Gaussian tree approximation (GTA) and expectation consistency (EC) attempt to approximate the true likelihood function under discrete finite-set constraints with a new distribution by minimizing the Kullback–Leibler (KL) divergence. As the KL divergence is not a true distance measure, ’exclusive’ and ’inclusive’ KL divergences are utilized by GTA and EC, respctively, demonstrating different performances. In this paper, we further combine the two asymmetric KL divergences in a nested way by proposing a generic algorithm framework named nested variational chain. Acting as an initial application, a MIMO detection algorithm named Gaussian tree approximation expectation consistency (GTA-EC) can thus be presented along with its alternative version for better understanding. With less computational burden compared to its counterparts, GTA-EC is able to provide better detection performance and diversity gain, especially for large-scale high-order MIMO systems. Full article

Endometrial cancer (EC) stands as the most prevalent gynecologic malignancy. In the past, it was classified based on its hormone sensitivity. However, The Cancer Genome Atlas has categorized EC into four groups, which offers a more objective and reproducible classification and has been shown to have prognostic and therapeutic implications. Hormonally driven EC arises from a precursor lesion known as endometrial hyperplasia, resulting from unopposed estrogen. EC is usually diagnosed through biopsy, followed by surgical staging unless advanced disease is expected. The typical staging consists of a hysterectomy with bilateral salpingo-oophorectomy and sentinel lymph node biopsies, with a preference placed on a minimally invasive approach. The stage of the disease is the most significant prognostic marker. However, factors such as age, histology, grade, myometrial invasion, lymphovascular space invasion, tumor size, peritoneal cytology, hormone receptor status, ploidy and markers, body mass index, and the therapy received all contribute to the prognosis. Treatment is tailored based on the stage and the risk of recurrence. Radiotherapy is primarily used in the early stages, and chemotherapy can be added if high-grade histology or advanced-stage disease is present. The risk of EC recurrence increases with advances in stage. Among the recurrences, vaginal cases exhibit the most favorable response to treatment, typically for radiotherapy. Conversely, the treatment of widespread recurrence is currently palliative and is best managed with chemotherapy or hormonal agents. Most recently, immunotherapy has emerged as a promising treatment for advanced and recurrent EC. Full article

This study aimed to assess the impacts of climate change on streamflow characteristics of five tropical catchments located in Costa Rica. An ensemble of five General Circulation Models (GCMs), namely HadGEM2-ES, CanESM2, EC-EARTH, MIROC5, MPI-ESM-LR dynamically downscaled by two Regional Climate Models (RCMs), specifically HadRM3P and RCA4, was selected to provide an overview of the impacts of different climate change scenarios under Representative Concentration Pathways (RCPs) 2.6, 4.5 and 8.5 using the 1961–1990 baseline period. The GR2M hydrological model was used to reproduce the historical monthly surface runoff patterns of each catchment. Following calibration and validation of the GRM2 model, the projected impact of climate change on streamflow was simulated for a near-future (2011–2040), mid-future (2041–2070) and far-future (2071–2100) for each catchment using the bias-corrected GCM-RCM multimodel ensemble-mean (MEM). Results anticipate wetter conditions for all catchments in the near-future and mid-future periods under RCPs 2.6 and 4.5, whereas dryer conditions are expected for the far-future period under RCP 8.5. Projected temperature trends indicate consistently warmer conditions with increasing radiative forcing and future periods. Streamflow changes across all catchments however are dominated by variations in projected precipitation. Wetter conditions for the near-future and mid-future horizons under RCPs 2.6 and 4.5 would result in higher runoff volumes, particularly during the late wet season (LWS). Conversely, dryer conditions for the far-future period under RCP8.5 would result in considerably lower runoff volumes during the early wet season (EWS) and the Mid-Summer Drought (MSD). In consequence, projected seasonal changes on streamflow across all catchments may result in more frequent flooding, droughts, and water supply shortage compared to historical hydrological regimes. Full article

Drought is one of the major environmental stressors; drought is increasingly threatening the living environment of mankind. The standardized precipitation evapotranspiration index (SPEI) with a 12-month timescale was adopted to monitor dry–wet status over China from 1951 to 2021. The modified Mann–Kendall (MMK) and Pettitt tests were used to assess the temporal trend and nonlinear behavior of annual drought variability. The analysis focuses on the spatio-temporal structure of the dry–wet transition and its general connections with climate change processes. In addition, the seasonal autoregressive integrated moving average (SARIMA) model was applied to forecast the dry–wet behavior in the next year (2022) at 160 stations, and the hotspot areas for extreme dryness–wetness in China were identified in the near term. The results indicate that the dry–wet climate in China overall exhibits interannual variability characterized by intensified drought. The climate in the Northeast China (NEC), North China (NC), Northwest China (NWC), and Southwest China (SWC) has experienced a significant (p < 0.05) drying trend; however, the dry–wet changes in the East China (EC) and South Central China (SCC) are highly spatially heterogeneous. The significant uptrend in precipitation is mainly concentrated to the west of 100° E; the rising magnitude of precipitation is higher in Eastern China near 30° N, with a changing rate of 20–40 mm/decade. Each of the sub-regions has experienced significant (p < 0.01) warming over the past 71 years. Geographically, the increase in temperature north of 30° N is noticeably higher than that south of 30° N, with trend magnitudes of 0.30–0.50 °C/decade and 0.15–0.30 °C/decade, respectively. The response of the northern part of Eastern China to the warming trend had already emerged as early as the 1980s; these responses were earlier and more intense than those south of 40° N latitude (1990s). The drying trends are statistically significant in the northern and southern regions, bounded by 30° N, with trend magnitudes of −0.30–−0.20/decade and −0.20–−0.10/decade, respectively. The northern and southwestern parts of China have experienced a significant (p < 0.05) increase in the drought level since the 1950s, which is closely related to significant warming in recent decades. This study reveals the consistency of the spatial distribution of variations in precipitation and the SPEI along 30° N latitude. A weak uptrend in the SPEI, i.e., an increase in wetness, is shown in Eastern China surrounding 30° N, with a changing rate of 0.003–0.10/decade; this is closely associated with increasing precipitation in the area. Drought forecasting indicates that recent drying areas are located in NWC, the western part of NC, the western part of SWC, and the southern part of SCC. The climate is expected to show wetting characteristics in NEC, the southeastern part of NC, and the eastern part of EC. The dry–wet conditions spanning the area between 30–40° N and 100–110° E exhibit a greater spatial variability. The region between 20–50° N and 80–105° E will continue to face intense challenges from drought in the near future. This study provides compelling evidence for the temporal variability of meteorological drought in different sub-regions of China. The findings may contribute to understanding the spatio-temporal effect of historical climate change on dry–wet variation in the region since the 1950s, particularly in the context of global warming. Full article

This experimental study presents concrete-filled double-skin tubular columns and demonstrates their expected advantages. These columns consist of an outer steel tube, an inner steel tube, and concrete sandwiched between two tubes. The influence of the outer-to-inner tube dimension ratio, outer tube to thickness ratio, and type of inner tube material (steel, PVC pipe) on the ultimate axial capacity of concrete-filled double-skin tubular columns is studied. It is found that the yield strength of the inner tube does not significantly affect the ultimate axial capacity of concrete-filled double-skin tubular composites. With the replacement of the inner tube of steel with a PVC pipe, on average, less than 10% strength is reduced, irrespective of size and dimensions of the steel tube. Hence, the cost of a project can be reduced by replacing inner steel tubes with a PVC pipes. Finally, the experimental results are compared with the existing design methods presented in AISC 360-16 (2016), GB51367 (2019), and EC4 (2004). It is found from the comparison that GB51367 (2019) gives better results, followed by AISC (2016) and EC4 (2004). Full article

Background: Ultrasonography’s usefulness in endometrial cancer (EC) diagnosis consists in its roles in staging and prediction of metastasis. Ultrasound-measured tumor-free distance from the tumor to the uterine serosa (uTFD) is a promising marker for these diagnostic and prognostic variables. The aim of the study was to determine the usefulness of this biomarker in locoregional staging, and thus in the prediction of lymph node metastasis (LNM). Methods: We conducted a single-institutional, prospective study on 116 consecutive patients with EC who underwent 2D transvaginal ultrasound examination. The uTFD marker was compared with the depth of ultrasound-measured myometrial invasion (uMI). Univariable and multivariable logit models were evaluated to assess the predictive power of the uTFD and uMI in regard to LNM. The reference standard was a final histopathology result. Survival was assessed by the Kaplan–Meier method. Results: LNM was found in 17% of the patients (20/116). In the univariable analysis, uMI and uTFD were significant predictors of LNM. The accuracy was 70.7%, and the NPV was 92.68% (OR 4.746, 95% CI 1.710–13.174) for uMI (p = 0.002), and they were 63.8% and 89.02% (OR 0.842, 95% CI 0.736–0.963), respectively, for uTFD (p = 0.01). The cutoff value for uTFD in the prediction of LNM was 5.2 mm. The association between absence of LNM and biomarker values of uMI < 1/2 and uTFD ≥ 5.2 mm was greater than that between the presence of metastases and uMI > 1/2 and uTFD values <5.2 mm. In the multivariable analysis, the accuracy of the uMI–uTFD model was 74%, and its NPV was 90.24% (p = non-significant). Neither uMI nor uTFD were surrogates for overall and recurrence-free survivals in endometrial cancer. Conclusions: Both uMI and uTFD, either alone or in combination, were valuable tools for gaining additional preoperative information on expected lymph node status. Negative lymph nodes status was better described by ultrasound biomarkers than a positive status. It was easier to use the uTFD rather than the uMI measurement as a biomarker of EC invasion, and the former still maintained a similar predictive value for lymph node metastases to the latter at diagnosis. Full article

The East China Sea (ECS) is the largest marginal sea in the northern western Pacific Ocean. In comparison to various physical studies, little information on the seasonal patterns in community structure of phytoplankton is currently available. Based on high performance liquid chromatography (HPLC) pigment analysis, spatiotemporal variations in phytoplankton community compositions were investigated in the northern ECS. Water temperature and salinity generally decreased toward the western part of the study area but warmer conditions in August led to strong vertical stratification of the water column. In general, major inorganic nutrient concentrations were considerably higher in the western part with a shallow water depth, and consistent with previous results, had no discernable vertical pattern during our observation period except in August. This study also revealed PO₄-limited environmental conditions in May and August. The monthly averaged integral chlorophyll-a concentration varied seasonally, highest (35.2 ± 20.22 mg m⁻²) in May and lowest (5.2 ± 2.54 mg m⁻²) in February. No distinct vertical differences in phytoplankton community compositions were observed for all the sampling seasons except in August when cyanobacteria predominated in the nutrient-deficient surface layer and diatoms prevailed at deep layer. Canonical correlation analysis results revealed that nutrient distribution and the water temperature were the major drivers of the vertical distribution of phytoplankton communities in August. Spatially, a noticeable difference in phytoplankton community structure between the eastern and western parts was observed in November with diatom domination in the western part and cyanobacteria domination in the eastern part, which were significantly (p < 0.01) correlated with water temperature, salinity, light conditions, and nutrient concentrations. Overall, the two major phytoplankton groups were diatoms (32.0%) and cyanobacteria (20.6%) in the northern ECS and the two groups were negatively correlated, which holds a significant ecological meaning under expected warming ocean conditions. Full article

This study aimed to translate and validate the Satter Eating Competence Inventory (ecSI2.0^TM) from English to Brazilian Portuguese. The process included three steps: (i) translation and back-translation of the original ecSI2.0^TM to Brazilian Portuguese; (ii) evaluation of its reproducibility; (iii) a pilot study to validate the Brazilian version of the Satter Eating Competence Inventory (ecSI2.0^TMBR) for a selected sample of the Brazilian adult population (internal consistency and factor validity). The reproducibility (test–retest reliability) was verified using the intraclass correlation coefficient (ICC) obtained by the responses of 32 Brazilian adults. All domains of the ecSI2.0^TMBR and the total score showed ICC > 0.8. Considering the entire questionnaire, none of the domains presented significant divergences among the participants’ responses (p < 0.001). In the pilot study with 662 individuals, 74.9% (n= 496) were female, mean age was 40.33 ± 12.55, and they presented a higher level of schooling and income. Analyses revealed Cronbach’s alpha coefficients of 0.869 for the ecSI2.0^TMBR total scale, 0.793 for Eating Attitudes, 0.527 for Internal Regulation, 0.728 for Food Acceptance, and 0.822 for Contextual Skills. In general, the ecSI2.0™BR presented good acceptability, showing total floor and ceiling effects of ≤0.6%. Factor validity was examined by confirmatory factor analysis. The four domains presented a good fit in the confirmatory factor analysis: RMSEA = 0.0123 (95% CI: 0–0.0266); CFI = 0.998; χ² = 75.9; df = 69; p = 0.266. The ecSI2.0^TMBR is the first tool designed to measure eating competence (EC) in the Brazilian population, showing good reproducibility and internal consistency. We expect the ecSI2.0^TMBR will support innovative research to investigate the association of EC and health outcomes, as well as new strategies based on emerging behavioral theories to enhance nutritional education policy. Full article

There are multiple factors affecting the behavior of water properties in an estuary, including the hydraulic properties of rivers and corresponding receiving water bodies, along with the potential solutes brought by rivers. Although there are various numerical models and analytical approaches to solving particular or holistic problems in estuaries, measurements are inevitably required. In this study, we developed an innovative low-cost probe based on the Arduino platform as an alternative to more expensive measuring systems. Our device is designed to measure position, temperature, and electrical conductivity in multiple realizations, and it consists of a floating container equipped with the following components: an Arduino Mega development board, a power management module, an SD card logging module, a Bluetooth module, a temperature measuring module, a global positioning satellite (GPS) position module, and a newly developed module for measuring electrical conductivity (EC). We emphasize that all used tools are open-source and greatly supported by the worldwide community. We tested our probe during a field campaign conducted at the estuary of River Jadro near Split (Croatia). Nine probes were released at the river mouth and their position, temperature, and EC were monitored and recorded during the experiment, which ended when the probes stopped, due to the river plume attenuation. The same experiment was repeated three times. All of the probes recorded consistent temperature data, while the EC data show more variable behavior, due to the higher sensitivity of the corresponding sensor. This was expected as a part of the natural process in the estuary. The measured data were additionally used to parameterize an analytical model for mean flow velocity and salinity as a proxy concentration. This showed a good match between the experimental results and the theoretical framework. This work, although focused on water surface applications in the near field zone of an estuary, should be considered as a promising step toward the development of innovative and affordable measurement devices. Full article

Pulmonary arterial hypertension (PAH) is a rare and progressive disease arising from various etiologies and pathogenesis. PAH decreases life expectancy due to pulmonary vascular remodeling, elevation of mean pulmonary arterial pressure, and ultimately progresses to heart failure. While clinical treatments are available to reduce the associated symptoms, a complete cure has yet to be found. Phosphodiesterase-5 (PDE-5) inhibition has been identified as a possible intervention point in PAH treatment. The functional vasodilation response to N²,N⁴-diamino quinazoline analogues with differing PDE-5 inhibitory activities and varying physicochemical properties were assessed in both endothelium-intact and denuded rat pulmonary arteries to gain greater insight into their mode of action. All analogues produced vasorelaxant effects with EC50s ranging from 0.58 ± 0.22 µM to ˃30 µM. It was observed that vasodilation response in intact vessels was highly correlated with that of denuded vessels. The ~10% drop in activity is consistent with a loss of the nitric oxide mediated cyclic guanosine monophosphate (NO/cGMP) pathway in the latter case. A moderate correlation between the vasodilation response and PDE-5 inhibitory activity in the intact vessels was observed. Experimental protocol using the alpha-adrenergic (α₁) receptor agonist, phenylephrine (PE), was undertaken to assess whether quinazoline derivatives showed competitive behavior similar to the α₁ receptor blocker, prazosin, itself a quinazoline derivative, or to the PDE-5 inhibitor, sildenafil. Competitive experiments with the α₁-adrenergic receptor agonist point to quinazoline derivatives under investigation here act via PDE-5 inhibition and not the former. The pre-incubation of pulmonary arterial rings with quinazoline test compounds (10 μM) reduced the contractile response to PE around 40–60%. The most promising compound (9) possessed ~32 folds higher selectivity in terms of vasodilation to its mammalian A549 cell cytotoxicity. This study provides experi0 0mental basis for PDE-5 inhibition as the mode of action for vasodilation by N²,N⁴-diamino quinazoline analogues along with their safety studies that may be beneficial in the treatment of various cardiovascular pathologies. Full article

We report the heterologous expression and molecular characterization of the first extremely halophilic alpha-glucosidase (EC 3.2.1.20) from the archaeon Haloquadratum walsbyi. A 2349 bp region (Hqrw_2071) from the Hqr. walsbyi C23 annotated genome was PCR-amplified and the resulting amplicon ligated into plasmid pET28b(+), expressed in E. coli Rosetta cells, and the resulting protein purified by Ni-NTA affinity chromatography. The recombinant protein showed an estimated molecular mass of 87 kDa, consistent with the expected value of the annotated protein, and an optimal activity for the hydrolysis of α-PNPG was detected at 40 °C, and at pH 6.0. Enzyme activity values were the highest in the presence of 3 M NaCl or 3–4 M KCl. However, specific activity values were two-fold higher in the presence of 3–4 M KCl when compared to NaCl suggesting a cytoplasmic localization. Phylogenetic analyses, with respect to other alpha-glucosidases from members of the class Halobacteria, showed that the Hqr. walsbyi MalH was most similar (up to 41%) to alpha-glucosidases and alpha-xylosidases of Halorubrum. Moreover, computational analyses for the detection of functional domains, active and catalytic sites, as well as 3D structural predictions revealed a close relationship with an E. coli YicI-like alpha-xylosidase of the GH31 family. However, the purified enzyme did not show alpha-xylosidase activity. This narrower substrate range indicates a discrepancy with annotations from different databases and the possibility of specific substrate adaptations of halophilic glucosidases due to high salinity. To our knowledge, this is the first report on the characterization of an alpha-glucosidase from the halophilic Archaea, which could serve as a new model to gain insights into carbon metabolism in this understudied microbial group. Full article