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Search Results (1,356)

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Keywords = exoskeletons

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19 pages, 1795 KB  
Article
A Cable-Driven Hip Exoskeleton with a Postural Control Strategy for Reinforcing Human Balance
by Giovanni Gerardo Muscolo, Michele Conconi, Alessandra Del Felice, Lorenzo Chiari and Nicola Sancisi
Machines 2026, 14(5), 484; https://doi.org/10.3390/machines14050484 (registering DOI) - 24 Apr 2026
Abstract
Balance loss in older adults often leads to severe consequences, and wearable exoskeletons may help restore postural stability. This paper presents a novel hip cable-driven exoskeleton designed to support balance recovery. The proposed postural control strategy implemented on the device is based on [...] Read more.
Balance loss in older adults often leads to severe consequences, and wearable exoskeletons may help restore postural stability. This paper presents a novel hip cable-driven exoskeleton designed to support balance recovery. The proposed postural control strategy implemented on the device is based on maintaining balance by reducing the center of mass displacement from its equilibrium condition. Loss of balance is analyzed using multibody human models both with and without the exoskeleton. Simulation results evaluating static and dynamic balance demonstrate the effectiveness of the proposed control strategy and support its feasibility for implementation in a real system. The simulations presented in this study will be compared with experimental results from human subjects in future work. Full article
(This article belongs to the Special Issue Design and Control of Assistive Robots)
18 pages, 2817 KB  
Article
Ultrathin Temporary Tattoo Electrodes Enable Prolonged Skin-Conformable EMG Sensing for Hip Exoskeleton Control
by Michele Foggetti, Marina Galliani, Andrea Pergolini, Aliria Poliziani, Emilio Trigili, Francesco Greco, Nicola Vitiello, Laura M. Ferrari and Simona Crea
Sensors 2026, 26(9), 2587; https://doi.org/10.3390/s26092587 - 22 Apr 2026
Viewed by 201
Abstract
Conventional gel electrodes are the gold standard for surface electromyography (sEMG), yet their bulkiness, stiffness, and limited gel lifetime prevents seamless day-long integration with wearable robots. We integrated ultrathin skin-conformal temporary tattoo electrodes with a powered unilateral hip exoskeleton and compared signal quality [...] Read more.
Conventional gel electrodes are the gold standard for surface electromyography (sEMG), yet their bulkiness, stiffness, and limited gel lifetime prevents seamless day-long integration with wearable robots. We integrated ultrathin skin-conformal temporary tattoo electrodes with a powered unilateral hip exoskeleton and compared signal quality during treadmill walking against gel. In this pilot study, five healthy participants completed three consecutive walking blocks at fixed speed: (1) using gel electrodes; (2) using tattoo electrodes to compare signal quality; and (3) using the same tattoo electrodes (not repositioned) after eight hours of wear to simulate a full day of typical device use and to evaluate potential degradation in signal quality over time. Electrodes were positioned on muscles not covered by the exoskeleton interface (tibialis anterior and gastrocnemius medialis), as well as on muscles located beneath the exoskeleton cuff, which were potentially subject to motion artifacts due to the application of external forces by the exoskeleton (rectus femoris and biceps femoris, BF). Across all muscles, for both gel and tattoo electrodes, the root mean square error (RMSE) between normalized sEMG envelopes and biological activation profile was 0.069 ± 0.048, and Pearson’s correlation coefficient (ρ) was 0.844 ± 0.091. Re-testing the same tattoo electrode pair after eight hours confirmed day-long stability without the need for recalibration. Statistical analysis revealed no significant differences in signal quality, also when applying assistive forces, between the two electrode types and across all muscles (RMSE, all p ≥ 0.3125; ρ, all p ≥ 0.1250), as well as no degradation after eight hours (RMSE and ρ: all p ≥ 0.0626, uncorrected). Finally, in a proof-of-concept session, BF activity measured with tattoo electrodes was found reliable to drive hip-extension assistance in real time. Collectively, these results show that tattoo electrodes deliver signal quality comparable to gel electrodes while offering a low-profile skin-conformal interface and day-long usability, making them a promising option for enhancing EMG-based control in wearable robots. Full article
(This article belongs to the Special Issue Advancing Medical Robotics Through Soft Sensing)
31 pages, 9719 KB  
Article
Nonlinear Dynamic Behavior and Kinematic Joint Wear Characteristics of a Bionic Humanoid Leg Mechanism with Multiple Revolute Joint Clearances
by Yilin Wang, Siyuan Zheng, Yiran Wei, Jianuo Zhu, Shuai Jiang and Shutong Zhou
Lubricants 2026, 14(4), 167; https://doi.org/10.3390/lubricants14040167 - 13 Apr 2026
Viewed by 234
Abstract
With the rapid advancement of exoskeletons and rehabilitation robotics, modern healthcare increasingly demands high dynamic accuracy and reliability from medical devices. However, the dynamic response and durability of mechanical systems are greatly influenced by the inevitable existence of clearances in kinematic joints. Existing [...] Read more.
With the rapid advancement of exoskeletons and rehabilitation robotics, modern healthcare increasingly demands high dynamic accuracy and reliability from medical devices. However, the dynamic response and durability of mechanical systems are greatly influenced by the inevitable existence of clearances in kinematic joints. Existing studies predominantly focus on simplified planar or spatial mechanisms, offering limited guidance for complex mechanical structures in medical applications. To address this issue, a unified modeling framework is proposed in this study to explore the nonlinear dynamic behavior and wear properties of bionic humanoid rigid mechanisms incorporating revolute joint clearances. A dynamic model that accounts for revolute joint clearances is established, employing the Lankarani–Nikravesh contact model alongside a refined Coulomb friction approach to characterize contact behavior. To characterize the wear progression between the shaft and the bushing, the Archard wear model is employed, while the system’s dynamic equations are formulated using the Lagrange multiplier approach. Systematic simulations are conducted to examine the effects of clearance size, location, and multi-clearance coupling on dynamic response and wear behavior. The results reveal that clearances at the hip joint have the most pronounced impact on system performance, tibiofemoral joint clearances exacerbate precision disturbances, and foot-end clearances considerably undermine system robustness. Increased clearance sizes and the coexistence of multiple clearances aggravate wear and induce more severe nonlinear dynamic phenomena. Phase portraits and Poincaré maps further illustrate that the system may exhibit complex or chaotic behavior under certain conditions. This study offers theoretical insights into performance degradation mechanisms in humanoid robots with joint clearances and introduces a modular “driving–mid–terminal” structure that enhances model generality, enabling its application to exoskeletons and rehabilitation devices for design optimization, service life prediction, and health monitoring. Full article
(This article belongs to the Special Issue Advances in Tribology and Lubrication for Bearing Systems)
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18 pages, 1695 KB  
Article
Trajectory Tracking Control of Lower Limb Rehabilitation Exoskeleton Robot Based on Adaptive-Weight MPC
by Linqi Zheng, Yuan Zhou, Anjie Mao and Shuwang Du
Actuators 2026, 15(4), 214; https://doi.org/10.3390/act15040214 - 11 Apr 2026
Viewed by 281
Abstract
In this paper, an adaptive-weight model predictive control (AW-MPC) strategy is proposed to address the trajectory tracking problem of a lower-limb rehabilitation exoskeleton robot. First, based on human motion analysis, the dynamics of the lower-limb rehabilitation exoskeleton are established, and the nonlinear dynamic [...] Read more.
In this paper, an adaptive-weight model predictive control (AW-MPC) strategy is proposed to address the trajectory tracking problem of a lower-limb rehabilitation exoskeleton robot. First, based on human motion analysis, the dynamics of the lower-limb rehabilitation exoskeleton are established, and the nonlinear dynamic model is transformed into a linear model. Second, a MPC objective function is formulated to minimize the tracking error, yielding the optimal control input. Then, on the basis of conventional MPC, a weight-tuning scheme is developed: a weighting function is constructed according to the evolution of the tracking error to adaptively adjust the MPC weighting coefficients, and the closed-loop stability of the control system is proven via a Lyapunov-based analysis. Finally, the proposed method is validated on a lower-limb rehabilitation exoskeleton experimental platform, with a PID controller designed as a baseline for comparison. The experimental results demonstrate that, compared with the PID controller, the proposed AW-MPC achieves faster convergence of the tracking error, higher tracking accuracy, and enhanced robustness. Full article
(This article belongs to the Special Issue Advanced Perception and Control of Intelligent Equipment)
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15 pages, 2199 KB  
Article
Constrained Dynamic Optimization of the Sit-to-Stand Task
by Amur AlYahmedi, Sarra Gismelseed and Riadh Zaier
Appl. Sci. 2026, 16(8), 3721; https://doi.org/10.3390/app16083721 - 10 Apr 2026
Viewed by 199
Abstract
This study develops a reduced-order predictive model of the Sit-To-Stand (STS) task to examine whether a simplified biomechanical representation can reproduce key STS patterns reported in the literature and to investigate the role played in movement by a flexible trunk. The model represents [...] Read more.
This study develops a reduced-order predictive model of the Sit-To-Stand (STS) task to examine whether a simplified biomechanical representation can reproduce key STS patterns reported in the literature and to investigate the role played in movement by a flexible trunk. The model represents the human body as a planar multibody system and formulates STS as an optimization problem within a discrete mechanics framework. This formulation combines reduced model complexity, explicit torso flexibility, and a structure-preserving numerical approach for trajectory generation. Simulations were used to evaluate the effects of movement duration, reduced joint strength, and seat height on joint torques, kinematics, trunk motion, and ground reaction forces (GRFs). The results reproduced several qualitative trends reported in previous experimental studies, including increased peak joint torques and GRFs with shorter movement duration, lower joint strength, and reduced seat height, as well as greater compensatory trunk motion under more demanding conditions. These findings suggest that the proposed framework captures key adaptive features of STS mechanics and may provide useful insights for rehabilitation analysis and the design of assistive technologies such as lower-limb exoskeletons and rehabilitation devices. At the same time, the present work should be regarded as an initial methodological study, since validation is currently qualitative and further experimental calibration, quantitative validation, and sensitivity analysis remain part of ongoing work. Full article
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15 pages, 2413 KB  
Article
A Motion Intention Recognition Method for Lower-Limb Exoskeleton Assistance in Ultra-High-Voltage Transmission Tower Climbing
by Haoyuan Chen, Yalun Liu, Ming Li, Zhan Yang, Hongwei Hu, Xingqi Wu, Xingchao Wang, Hanhong Shi and Zhao Guo
Sensors 2026, 26(8), 2346; https://doi.org/10.3390/s26082346 - 10 Apr 2026
Viewed by 306
Abstract
Transmission tower climbing is a critical specialized operation in ultra-high-voltage power maintenance and communication infrastructure servicing. However, existing lower-limb exoskeletons used for tower climbing still suffer from insufficient motion intention recognition accuracy under complex operational environments. To address this issue, this study proposes [...] Read more.
Transmission tower climbing is a critical specialized operation in ultra-high-voltage power maintenance and communication infrastructure servicing. However, existing lower-limb exoskeletons used for tower climbing still suffer from insufficient motion intention recognition accuracy under complex operational environments. To address this issue, this study proposes an inertial measurement unit (IMU)-based bidirectional temporal deep learning method for motion intention recognition. First, a one-dimensional convolutional neural network (1D-CNN) is employed to extract local temporal features from multi-channel IMU signals. Subsequently, a bidirectional long short-term memory network (Bi-LSTM) is introduced to model the forward and backward temporal dependencies of motion sequences. Furthermore, a temporal attention mechanism is incorporated to emphasize discriminative features at critical movement phases, enabling the precise recognition of short-duration and transitional motions. Experimental results demonstrate that the proposed method outperforms traditional machine learning approaches and unidirectional temporal models in terms of accuracy, F1-score, and other evaluation metrics. In particular, this method demonstrates significant advantages in identifying the flexion/extension phases and transitional states. This study provides an offline method for analyzing movement intentions in lower-limb exoskeleton control for power transmission tower climbing scenarios and offers a reference for developing assistive control strategies for assisted climbing tasks in this specific context. Full article
(This article belongs to the Section Electronic Sensors)
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17 pages, 5304 KB  
Article
Design and Experimental Evaluation of a Shoulder Assistive Exoskeleton for Insulator Replacement
by Haoyuan Chen, Jia Yao, Ming Li, Hongwei Hu, Zhan Yang, Siyu Tu, Yalun Liu, Zimeng Wang and Zhao Guo
Sensors 2026, 26(8), 2313; https://doi.org/10.3390/s26082313 - 9 Apr 2026
Viewed by 327
Abstract
Aiming to reduce muscle fatigue and prevent occupational injuries caused by prolonged lifting in insulator replacement operations, this study presents the design of an upper-limb exoskeleton. Firstly, this study performs kinematic analysis and phase segmentation of the lifting motion in the insulator replacement [...] Read more.
Aiming to reduce muscle fatigue and prevent occupational injuries caused by prolonged lifting in insulator replacement operations, this study presents the design of an upper-limb exoskeleton. Firstly, this study performs kinematic analysis and phase segmentation of the lifting motion in the insulator replacement operation. Based on the analysis, in terms of mechanical structure, the proposed upper-limb exoskeleton adopts a unilateral three-degree-of-freedom shoulder mechanism that biomimics the human glenohumeral joint, which reduces the misalignment between the exoskeleton and the human body. Meanwhile, a waist–back support structure is integrated into the exoskeleton to realize a more reasonable torque transmission path. In terms of the control strategy, based on the operation’s phase segmentation and dynamic modeling of the human upper limb, this study develops a neural network-based assistive control algorithm for insulator replacement operations, enabling the exoskeleton to provide phase-specific torque output. Experimental results demonstrate that, under a simulated insulator replacement operation with a 20 kg load, the exoskeleton significantly reduces the subject’s sEMG activity of the biceps brachii and triceps brachii, effectively alleviating muscle fatigue. Full article
(This article belongs to the Section Sensors and Robotics)
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2 pages, 124 KB  
Editorial
Special Issue “AI for Robotic Exoskeletons and Prostheses”
by Claudio Loconsole
Robotics 2026, 15(4), 77; https://doi.org/10.3390/robotics15040077 - 7 Apr 2026
Viewed by 317
Abstract
This Special Issue was conceived to explore how Artificial Intelligence can meaningfully empower robotic exoskeletons and prosthetic systems, enhancing modeling, control, perception, and real-world applicability to ultimately improve the quality of life of individuals that rely on these technologies [...] Full article
(This article belongs to the Special Issue AI for Robotic Exoskeletons and Prostheses)
25 pages, 5534 KB  
Article
Task-Dependent Effectiveness of a Quasi-Direct-Drive Upper-Limb Exoskeleton: Shoulder Muscle Offloading Versus Metabolic Cost in Overhead Work
by Yongxuan Hong, Jiying Du, Sida Du, Yue Ma, Xiangyang Wang and Chunjie Chen
Bioengineering 2026, 13(4), 423; https://doi.org/10.3390/bioengineering13040423 - 3 Apr 2026
Viewed by 470
Abstract
Work-related shoulder disorders during overhead assembly represent a persistent occupational challenge. We evaluated a quasi-direct-drive (QDD) active upper-limb exoskeleton during simulated overhead work, providing simultaneous metabolic, electromyographic, and kinematic assessment of QDD actuation under static and dynamic conditions. Seven healthy males completed within-subject [...] Read more.
Work-related shoulder disorders during overhead assembly represent a persistent occupational challenge. We evaluated a quasi-direct-drive (QDD) active upper-limb exoskeleton during simulated overhead work, providing simultaneous metabolic, electromyographic, and kinematic assessment of QDD actuation under static and dynamic conditions. Seven healthy males completed within-subject comparisons of without-exoskeleton (WO) and with-exoskeleton (WE) conditions during dynamic screwing (5 min) and static holding (2.5 min, 3 kg). During static holding, the exoskeleton achieved substantial shoulder offloading (Upper Trapezius: −68.2%, 6/6 participants, p = 0.031, d = 3.61; Anterior Deltoid: −43.6%) and improved postural stability (32–41% variability reduction). However, metabolic cost increased during both static (+57.2%) and dynamic (+30.6%) tasks, while movement smoothness degraded. These findings extend prior task-dependent exoskeleton observations to QDD actuation, revealing that intrinsic backdrivability does not eliminate whole-body energy penalties from device mass. The exoskeleton exhibits task-dependent effectiveness: potentially suitable for prolonged static overhead holding but not currently recommended for dynamic assembly without mass reduction and control refinement. Full article
(This article belongs to the Special Issue Advanced Wearable Sensors for Human Gait Analysis)
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17 pages, 6541 KB  
Article
Active-Assistive Control Based on Dynamic Moving Window for Trajectory Tracking of an Upper Limb Exoskeleton in Assisted Rehabilitation
by Yuseop Sim, Jaehwan Kong, Seong-Sig Choi and Hak Yi
Sensors 2026, 26(7), 2160; https://doi.org/10.3390/s26072160 - 31 Mar 2026
Viewed by 401
Abstract
Rehabilitation robotics faces the challenges of aligning engineering design with patient-specific needs. Most existing controllers in rehabilitation robots often constrain motion to fixed paths or provide only passive guidance, limiting user engagement and adaptability. This study proposes a novel active-assistive mode controller that [...] Read more.
Rehabilitation robotics faces the challenges of aligning engineering design with patient-specific needs. Most existing controllers in rehabilitation robots often constrain motion to fixed paths or provide only passive guidance, limiting user engagement and adaptability. This study proposes a novel active-assistive mode controller that integrates a virtual tunnel-based force generation mechanism with a dynamic moving-window technique for tracking activities of daily living (ADL) trajectories. Unlike conventional impedance controllers, the proposed method dynamically adjusts the virtual tunnel in real time, permitting voluntary upper-limb movement within a safe operational range while preventing excessive deviation. The system was implemented on a wearable two-degree-of-freedom (DOF) upper-limb exoskeleton equipped with drive and integrated sensor units. Experimental results demonstrated that decreasing the guidance force (Fgf) increased tracking errors, from 1° at 100% Fgf to 5° at 30% Fgf, indicating greater voluntary participant motion. Peak actuator torques correspondingly decreased from 14.75 to 13.43 Nm (elbow) and from 4.14 to 2.48 Nm (wrist), confirming the controller’s capability to modulate robotic assistance according to user effort. Tests with 30 healthy participants demonstrated the effectiveness of guidance along predefined ADL trajectories, validating the controller’s potential for patient-centered rehabilitation. Full article
(This article belongs to the Special Issue Dynamics and Control System Design for Robotics)
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24 pages, 8557 KB  
Article
Dynamic Modelling and Control Strategy Analysis of a Lower-Limb Exoskeleton
by Huanrong Xiao, Teng Ran and Afang Jin
Sensors 2026, 26(7), 2124; https://doi.org/10.3390/s26072124 - 29 Mar 2026
Viewed by 436
Abstract
Lower-limb exoskeleton robots play a pivotal role in rehabilitation medicine and assistive augmentation, where precise dynamic modelling and trajectory tracking control are fundamental to effective assistance. Existing models predominantly focus on hip and knee rotational degrees of freedom, with insufficient attention to ankle [...] Read more.
Lower-limb exoskeleton robots play a pivotal role in rehabilitation medicine and assistive augmentation, where precise dynamic modelling and trajectory tracking control are fundamental to effective assistance. Existing models predominantly focus on hip and knee rotational degrees of freedom, with insufficient attention to ankle dynamics and pelvic translation. To address these limitations, this paper establishes a sagittal-plane dynamic model comprising nine generalised coordinates, treating the human lower limb and exoskeleton as an integrated coupled system. A seven-segment kinematic model encompassing the trunk, bilateral thighs, shanks, and feet is constructed via a modified Denavit–Hartenberg parameter method, and dynamic equations are derived using Lagrangian formulation. Three control strategies—PD control, PD with gravity compensation, and the computed torque method—are designed and evaluated through simulations using gait data from five subjects (two self-collected, three from a public dataset) acquired via Vicon motion capture. Results demonstrate that the computed torque method achieves a joint angle tracking root mean square error (RMSE) of 0.59°, representing an 86.3% improvement over conventional PD control, while maintaining a low control torque RMS of 4.44 N·m. The controller exhibits stable tracking performance across walking speeds of 0.4–1.45 m/s, validating the effectiveness of the proposed model and control strategies. Full article
(This article belongs to the Section Sensors and Robotics)
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39 pages, 18846 KB  
Article
Integrated Design of a Modular Lower-Limb Rehabilitation Exoskeleton: Multibody Simulation, Load-Driven Structural Optimization, and Experimental Validation
by Ionut Geonea, Andrei Corzanu, Cristian Copilusi, Adriana Ionescu and Daniela Tarnita
Robotics 2026, 15(4), 71; https://doi.org/10.3390/robotics15040071 - 28 Mar 2026
Viewed by 499
Abstract
Lower-limb rehabilitation exoskeletons must balance biomechanical compatibility, structural safety, and low mass to enable practical, repeatable gait assistance. This paper proposes a planar pantograph-derived exoskeleton leg driven by a Chebyshev Lambda linkage and develops an integrated workflow from mechanism synthesis to manufacturable optimization [...] Read more.
Lower-limb rehabilitation exoskeletons must balance biomechanical compatibility, structural safety, and low mass to enable practical, repeatable gait assistance. This paper proposes a planar pantograph-derived exoskeleton leg driven by a Chebyshev Lambda linkage and develops an integrated workflow from mechanism synthesis to manufacturable optimization and experimental verification. A mannequin-coupled multibody model was built in MSC ADAMS to evaluate joint kinematics, end-point (foot) trajectories, and joint reaction forces under multiple scenarios (fixed-frame, ramp, stair ascent, and inclined-plane walking). The extracted joint loads were transferred to a parametric finite element model in ANSYS Workbench 2019, where response surface surrogates and a multi-objective genetic algorithm (MOGA) were used to minimize mass under stiffness and strength constraints. For the optimized load-bearing link, the selected minimum-mass design reached a component mass of 0.542 kg while respecting the imposed structural limits, i.e., a maximum total deformation below 0.2 mm and a maximum equivalent (von Mises) stress below 50 MPa (e.g., ~0.188 mm deformation and ~39 MPa stress in the optimal candidate). A rapid prototype was manufactured by 3D printing and experimentally evaluated using CONTEMPLAS high-speed video tracking, providing measured XM(t) and YM(t) trajectories and joint-angle histories for quantitative comparison with simulations via RMSE metrics. Full article
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25 pages, 1648 KB  
Review
Freezing of Gait in Parkinson’s Disease: A Scoping Review on the Path Towards Real-Time Therapies
by Meenakshi Singhal, Christina Grannie, Margaret Burnette, Manuel E. Hernandez and Samar A. Hegazy
Sensors 2026, 26(7), 2042; https://doi.org/10.3390/s26072042 - 25 Mar 2026
Viewed by 607
Abstract
Background: Freezing of gait (FoG) is a common symptom of Parkinson’s disease, especially in its later stages of progression. Characterized by involuntary stopping during normal gait patterns, FoG greatly increases fall risk, reducing quality of life. Given the complex presentation and etiology of [...] Read more.
Background: Freezing of gait (FoG) is a common symptom of Parkinson’s disease, especially in its later stages of progression. Characterized by involuntary stopping during normal gait patterns, FoG greatly increases fall risk, reducing quality of life. Given the complex presentation and etiology of FoG, current treatments have proven ineffective in managing episodes. In recent years, machine learning algorithms have been leveraged to derive actionable clinical insights from biomedical datasets. As a manifestation of neuromechanical dysfunction, impending FoG episodes may be characterized through data collected by wearable devices and sensors. Objective: This scoping review evaluates the current landscape of machine and deep learning-derived biomarkers to enhance the personalized management of FoG. Methods: This scoping review was conducted using established methodological frameworks for scoping reviews and is reported in accordance using the PRISMA-ScR checklist. Three databases were queried, with screening yielding 60 studies. Results: Thirty-nine papers reported on deep learning techniques, with the most common architectures being convolutional neural networks and long short-term memory models. Conclusions: Inertial measurement units, which can be worn on various locations, may be a promising modality for practical implementation. To generate closed-loop FoG therapies, algorithms can be integrated into real-time systems like robotic exoskeletons or adaptive deep brain stimulation. Future work in generating datasets from ambulatory devices, as well as distributed computing strategies, may lead to real-time FoG management. Full article
(This article belongs to the Special Issue Flexible Wearable Sensors for Biomechanical Applications)
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16 pages, 3479 KB  
Article
The Papilla Stage as a Critical Molecular Transition: Antp and Sex-Regulatory Network Orchestrate Cheliped Regeneration in Eriocheir sinensis
by Benzhen Li, Yanan Yang, Mengqi Ni, Yourong Liu and Zhaoxia Cui
Animals 2026, 16(6), 982; https://doi.org/10.3390/ani16060982 - 21 Mar 2026
Viewed by 301
Abstract
Cheliped regeneration in the E. sinensis is a tightly regulated physiological process, yet the molecular regulatory mechanisms underlying sexual dimorphism during regeneration remain unclear. In this study, we combined morphological observation with transcriptomic analysis to systematically investigate the regenerative stage characteristics and sex-related [...] Read more.
Cheliped regeneration in the E. sinensis is a tightly regulated physiological process, yet the molecular regulatory mechanisms underlying sexual dimorphism during regeneration remain unclear. In this study, we combined morphological observation with transcriptomic analysis to systematically investigate the regenerative stage characteristics and sex-related differences. The papilla stage 4 dpa was identified as a pivotal transitional stage, bridging initial wound healing and cellular dedifferentiation (2 dpa) with subsequent redifferentiation and morphogenesis (7 dpa). Morphological sex-based differences characterized by larger regenerating chelipeds in males became prominent by the late stage (28 dpa). Notably, the molecular foundation of sexual dimorphism was found to be established at 4 dpa, significantly preceding the emergence of phenotypic differences. This early divergence was driven by sex-dimorphic endocrine networks: males exhibited preferential expression of genes such as Fem-1c-like, Cyp2L1-like, CpAMP1A-like and Nedd4-like, while females showed enrichment in elevated aromatase activity. Weighted gene co-expression network analysis (WGCNA) identified the Hox gene Antp as a core hub regulator, exhibiting high co-expression with key epidermal-related genes such as Cht6, Cht2-like and more. Its suppressed expression at 2 dpa aligned with the requirements for dedifferentiation, whereas its peak at 4 dpa indicated a crucial role in orchestrating appendage patterning and exoskeleton assembly. RNA interference (RNAi) knockdown of Antp resulted in obscured differentiation between the propodus and carpus in both sexes and confirmed its regulatory control over downstream targets including Ubx, Bmp2-like, and CpAMP1A-like. This study suggests a putative hierarchical regulatory model in which systemic hormonal signals may integrate Antp and other sex-biased regulators to potentially facilitate structured limb regeneration. These findings offer tentative novel insights into the interplay between developmental plasticity and sex-based regulatory divergence in decapod crustaceans. Full article
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18 pages, 4206 KB  
Article
Aggregated vs. Isolated Seismic Response of a Historic Masonry Compound Before and After Integrated Retrofit Interventions
by Giovanna Longobardi and Antonio Formisano
Buildings 2026, 16(6), 1208; https://doi.org/10.3390/buildings16061208 - 18 Mar 2026
Viewed by 239
Abstract
The evaluation of the seismic behavior of masonry aggregates, which characterize Italian historic centres, is a challenging and widely debated topic in the field of structural engineering. These constructions, composed of several adjacent structural units, tend to exhibit both global and local damage [...] Read more.
The evaluation of the seismic behavior of masonry aggregates, which characterize Italian historic centres, is a challenging and widely debated topic in the field of structural engineering. These constructions, composed of several adjacent structural units, tend to exhibit both global and local damage when subjected to horizontal seismic actions—loads that were not considered at the time of their original construction. Developed over centuries of unplanned urban growth, they are based on empirical construction rules and locally sourced materials. Due to their poor thermal properties, these buildings are also affected by significant heat losses, resulting in reduced indoor comfort. In this context, the present study aims to evaluate the seismic performance of a masonry aggregate and two of its constituent structural units located in Visso, in the province of Macerata, an area severely affected by the 2016 Central Italy seismic sequence, both before and after the application of an innovative integrated retrofitting solution. The proposed strengthening system combines aluminium alloy exoskeleton with insulating sandwich panels, simultaneously addressing seismic vulnerability and energy inefficiency. The assessment is carried out through numerical analyses, including nonlinear static and dynamic approaches, to achieve a comprehensive understanding of the structural response. Moreover, a comparative analysis between the masonry aggregate and the two individual structural units, modelled as isolated buildings, is performed to investigate the influence of structural interaction among adjacent units. The results demonstrate the effectiveness of the proposed retrofitting strategy, highlighting a significant improvement in global stability. Furthermore, the comparison confirms the critical role of inter-unit interaction and underscores the necessity of modelling historic masonry aggregates rather than isolated buildings to obtain a more realistic seismic performance evaluation. Full article
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