Search Results (667)

Hypertension (HTN) and chronic kidney disease (CKD) are significant global health burdens, with microalbuminuria (MA) serving as a key early marker of renal damage and cardiovascular risk. While nutritional interventions are pivotal for management, the evidence for specific nutrients is often complex and inconsistent, creating challenges for clinical guidance. This review critically evaluates current evidence on the interaction among macronutrients, micronutrients, and established dietary approaches and their influence on the development and course of HTN and MA. Strong consensus is present regarding sodium restriction, increased intakes of potassium, and the implementation of dietary patterns like Dietary Approaches to Stop Hypertension (DASH) and the Mediterranean diet to improve blood pressure and renal outcomes. Evidence favors protein moderation (approximately 0.8 g/kg/day), especially from plant sources, and emphasizes carbohydrate quality (e.g., high fiber, low glycemic index) over absolute quantity. The role of micronutrients is more nuanced; maintaining vitamin D sufficiency is protective, but intervention trials for many supplements, including B vitamins and antioxidant vitamins (C and E), have yielded inconsistent results. Several minerals, such as iron and selenium, exhibit a U-shaped risk curve where both deficiency and excess are detrimental, highlighting the risks of unselective supplementation. Ideal nutrition care prioritizes holistic dietary patterns over a focus on single nutrients. Clinical guidance should be founded on sodium reduction and potassium-rich foods, with personalized recommendations for protein and micronutrient supplementation based on an individual’s specific cardiovascular and renal profile. Future research must target nutrients with conflicting evidence to establish clear, evidence-based intake guidelines. Full article

In this study, iron ore tailings (IOTs) with different mass replacement rates (0%, 20%, 40%, 60%, 80%, 100%) and basalt fibers (BFs) with different volume contents (0, 0.1%, 0.2%, 0.3%) were co-incorporated into recycled concrete. To better simulate real-world conditions, a coupled carbonation–freeze–thaw cycling test was performed on C30 cubic specimens. Each test cycle comprised 7 days of carbonation followed by 25 freeze–thaw cycles, with four total cycles conducted. For specimens subjected to different numbers of test cycles, measurements were taken of the recycled concrete’s variations in mass, dynamic elastic modulus, and compressive strength. Scanning electron microscopy (SEM) was employed to examine the microscopic morphology of concrete under the test conditions and to analyze the mechanism through which the two materials influence the durability of recycled concrete in the experimental environment. Based on the Weibull distribution, a damage prediction model for basalt fiber iron ore tailing recycled concrete (BF-IOT-RAC) under the test environment was developed, and the service life of BF-IOT-RAC in Northwest China was predicted. The results indicate that the two materials can enhance the performance of recycled concrete when their dosages are appropriate; however, excessive dosages exert adverse effects. BF1T40 had a mass loss rate of 1%, an RDEM of 92%, and the cube compressive strength of 33.5 MPa at the conclusion of this test, with all three indicators being higher than those of recycled concrete with a single material incorporated. SEM observations revealed that the surface of BF1T40 was more intact than that of other recycled concretes after the test. According to the prediction, BF1T40 has the longest service life in the northwest region, reaching 42–43 years. Full article

Background/Objectives: Ferroptosis, an iron-dependent form of regulated cell death characterized by excessive lipid peroxidation, has been implicated in various acute and chronic brain disorders, including epilepsy. Although 1,4-naphthoquinone derivatives have been reported to regulate ferroptosis, their mechanistic roles in the nervous system remain underexplored. Here, we investigated the protective effects of 8-hydroxy-2-anilino-1,4-naphthoquinone (8-HANQ) on ferroptotic neuronal death in vitro and seizure behaviors in vivo. Methods: HT22 hippocampal cells were exposed to ferroptosis inducers including glutamate, glutamate plus iron, or RSL3. Lipid reactive oxygen species (ROS), ferroptosis markers, and its related molecules were assessed by flow cytometry and Western blotting. In a kainate (KA)-induced seizure model, 8-HANQ was delivered intracerebroventricularly, followed by behavioral seizure scoring and analysis of hippocampal levels of PSD95, cathepsin-B, and FGFR1 at 72 h post-seizure. Results: 8-HANQ attenuated ferroptotic death in HT22 cells, reducing lipid ROS accumulation and abnormal acyl-coA synthetase long chain family member 4 (ACSL4), suggesting 8-HANQ’s anti-ferroptotic action. Moreover, 8-HANQ also prevented aberrant STAT3-dependent cathepsin-B overexpression while modulating soluble N-cadherin-mediated FGFR1 activation. In vivo, 8-HANQ decreased KA-induced seizure behavior, restored hippocampal cathepsin-B and PSD95 expression, and partially alleviated dysregulation of FGFR1 activation. Conclusions: 8-HANQ prevents ferroptotic neuronal death and synaptic deficits involving FGFR1/STAT3/cathepsin-B-driven ferroptosis while lowering seizure severity, suggesting that 8-HANQ may serve as a potential anti-ferroptotic and anti-seizure agent. Full article

Background: The increase in potentially toxic elements (PTEs) in the soil is worrying, especially in agricultural soils due to the bioaccumulation factor. Copper (Cu) and iron (Fe) are micronutrients, responsible for important functions in the plant body, but the high availability of these elements in the soil can cause soil contamination and toxicity in plants; consequently, they can be considered PTEs. Objectives: The focus of this study is to understand the physiological responses (pigments, gas exchange, growth, biomass, accumulation) of Canavalia ensiformis to high levels of Cu and Fe in the soil, in isolation, and to identify which PTE is most harmful to its development. Methods: Two experiments (Cu and Fe) were conducted simultaneously in a greenhouse. Treatments of 50, 150, 250, and 350 mg dm⁻³ of soil for each element (CuSO₄*5H₂O and FeSO₄*7H₂O) were incorporated into the soil (Oxisol) of each experimental unit (4 dm³ pot), in addition to the control. C. ensiformis seeds were sown directly in soil enriched with Cu and Fe, respectively, and after emergence they were cultivated for 90 days. Results: Changes in chlorophyll levels caused direct effects on gas exchange, shoot biomass, root development, nodulation, and total plant biomass. The tolerance of the species is dependent on chlorophyll levels and gas exchange. There was accumulation of both PTEs in the roots and low translocation to the shoot. Conclusions: The plants were tolerant to Fe treatments; however, they were not tolerant to Cu treatments (T150–T350). Excess Cu was more detrimental to plant development. Full article

Chlorinated benzoquinones, such as 2,6-dichlorobenzoquinone (DCBQ), are toxic disinfection byproducts of growing concern in aquatic environments. Advanced oxidation processes, particularly photo-Fenton treatment, provide sustainable alternatives for their degradation. However, optimization is required to ensure not only the removal of the parent compound but also the reduction in harmful intermediates. This study evaluated the degradation of DCBQ (1.0 mM H₂O₂, 150 W UV, pH 3.0, 25 °C) with ferrous ion between 0 and 1.0 mg/L. DCBQ removal followed a second-order kinetic model, reaching complete degradation. Aromaticity-loss and water color degradation adjusted to kinetics of second-order, reflecting the sequential reduction in chlorinated hydroquinones and chlorophenols type intermediates, with marked decreases after 120 min at 0.8 mg/L. Results showed that increasing iron dosage enhanced both the rate of DCBQ disappearance and the removal of aromaticity, with complete pollutant degradation. Importantly, optimal ferrous ion dosages (20 mol DCBQ: 70 mol H₂O₂: 1 mol Fe²⁺) effectively limited the persistence of intermediates, as evidenced by significant decreases in color and aromaticity, while avoiding excessive turbidity. These findings demonstrate that fine-tuning iron dosage in photo-Fenton systems can maximize contaminant elimination and minimize secondary byproducts, reinforcing their role as sustainable solutions for wastewater remediation. Full article

Objectives: This study utilized IPEC-J2, a neonatal pig jejunum-derived cell line, to assess how iron deficiency (ID) and excess (IE) alter enterocyte metabolism and the transcription of inflammatory markers. Methods: Cells were treated with deferiprone (DFP) or ferric ammonium citrate (FAC) to induce ID or IE, respectively. The study evaluated: (1) transcriptional changes in iron-regulatory genes over 96 h under ID or IE; (2) the interaction between iron imbalance and lipopolysaccharide (LPS) exposure on mRNA expression of inflammation markers and iron transporters; and (3) cellular metabolic responses to ID, IE, and iron repletion using untargeted metabolomics. Results: ID triggered dynamic transcriptional changes in iron regulatory genes and suppressed cellular proliferation via impaired DNA replication. IE resulted in a persistent reduction in TFRC expression. LPS increased CYBRD1 (p < 0.001) and IL8 (p = 0.004) and tended to elevate TLR4 and TNF expression (p ≤ 0.07), while iron deficiency upregulated IL8 expression (p < 0.001). ID disrupted the TCA cycle, reduced glucuronic acid synthesis, and elevated glycolysis for energy production, whereas IE increased cholesterol biosynthesis and decreased alpha-tocopherol levels. Repletion of iron partially reversed ID-induced metabolic changes. Conclusions: ID impaired enterocyte proliferation and profoundly disrupted cellular metabolism, whereas IE enhanced cholesterol synthesis and depleted alpha-tocopherol levels. Restoration of cellular metabolism following iron repletion was observed, highlighting the resilience of enterocytes. Full article

Eutrophication of freshwater bodies is primarily caused by excessive nitrogen and phosphorus, resulting in significant environmental challenges, including harmful algal blooms and hypoxia. This review examines the potential for natural and modified zeolites to act as adsorbents and regulate nutrient concentrations in eutrophic freshwater ecosystems, excluding applications for wastewater or industrial water effluents. Natural zeolites are effective adsorbents of ammonium, whereas modified zeolites (with aluminum, iron, calcium, and many others) have been noted to have enhanced phosphate adsorption and a higher overall nutrient removal efficiency. The application of modified zeolites for controlling eutrophication in freshwater bodies has proven to have high efficiency in adsorbing nitrogen and phosphorus, resulting in reduced nutrient release from sediments and improved water quality in shallow lakes and reservoirs. This review describes the adsorption mechanisms and modification methods, with an appreciation for the multifunctional role of zeolites in nutrient immobilization and capping sediments. Finally, it presents the potential to use zeolite-based materials in eutrophic freshwater restoration through sustainable circular economy approaches. Zeolite materials present ample environmental applications for cost-effective and targeted mitigation approaches to freshwater eutrophication. Full article

The steel industry is responsible for 7–9% of global CO₂ emissions. Shifting from primary iron ore to recycled scrap in electric arc furnace (EAF) steelmaking offers significant decarbonization potential, reducing carbon intensity by 60–70%. However, increased scrap use in EAF operations leads to higher nitrogen absorption, which can degrade mechanical properties. Nitrogen dissolves into molten steel, where it forms Cottrell atmospheres at dislocations in the following processing steps, intensifying strain aging and reducing ductility. This study establishes a precipitation criterion based on the TiN solubility product to prevent harmful liquid TiN formation, enabling effective nitrogen fixation via fine TiN precipitates (5–20 nm). Multiscale characterization techniques, such as TEM and EBSD, show that Ti reduces the number of mobile N atoms by 60–70%, evidenced by a 50–65% decrease in Snoek/SKK peak intensities. Excessive titanium can refine ferrite grain size and prevents harmful TiN inclusions. Titanium microalloying presents a cost-effective, sustainable strategy to reduce strain aging in scrap-rich EAF steels, enabling more sustainable steel production without sacrificing material properties. Full article

Black rhinoceros (Diceros bicornis) face threats to survival due to poaching in the wild and an incomplete understanding of preventive health monitoring under human care. Black rhinos under human care develop iron overload disorder (IOD) which is associated with predisposition to other disease, compromised immune function, hemolytic crisis, and death. Management of IOD is challenging but has been mitigated in some cases with dietary intervention and phlebotomy and documented through serum biomarker evaluation. Chelation therapy to reduce iron is rare in rhinos partially because of limited product availability and route of administration. An iron-specific chelator HBED (N,N’-Di(2-hydroxybenzyl)ethylenediamine-N,N’-diacetic acid) was investigated for oral use in southern black rhinos (n = 3) after successful testing was performed with equids as a model. Using a cross-over design, we tested the efficacy of short-term HBED administration. HBED was dosed at 40 mg/kg body weight for 10 days and resulted in increased urinary excretion of iron but unaltered fecal iron excretion in rhinos compared to control trials. Two rhinos maintained blood chemistry and cell distribution considered normal for the species. The third rhino experienced a hemolytic event after stopping HBED administration in the same time frame and at the same dose as the two conspecifics but fully recovered. Careful monitoring and tapering the drug at the completion of treatment is warranted, especially if the rhino’s iron load is considered high. HBED’s potential to induce iron excretion safely, as well as prevent excessive dietary iron uptake, may significantly benefit the black rhino population under human care. Full article

Sex differences are known to influence both micronutrient metabolism and cardiometabolic health outcomes. However, the extent to which these sex-specific factors interact, particularly in the relationship between micronutrient status and cardiometabolic risk, remains insufficiently understood. The present study aimed to investigate sex differences in health and micronutrient status, and their association with blood lipids, body mass index, and body weight. The final sample size included 488 Austrian adults (median age: 38 years; comprising 256 males and 232 females) who successfully met the inclusion criteria. Blood analyses were conducted to measure whole-blood micronutrients and serum levels of various cardiometabolic health biomarkers, including blood lipids. Micronutrient status (including deficiencies and excesses) was calculated using sex-specific reference ranges. There were significant differences between males and females for most (19 out of 28) blood biomarkers of cardiometabolic health (p < 0.05), with females generally showing a healthier cardiometabolic profile. Significant sex differences were also observed in the adjusted values of three micronutrients: copper was higher in females, while iron and vitamin B₁₂ were higher in males (p < 0.05). Sex differences in micronutrient status indicated a higher prevalence of iron deficiency and copper excess among females (p < 0.05). Different patterns were observed between males and females in the association of blood lipids and body composition with micronutrients and demographic factors. These findings highlight the importance of individualized approaches in preventive and therapeutic health interventions based on differences between males and females. Full article

Background. Melioidosis is a severe infection caused by Burkholderia pseudomallei and is endemic in regions with a high prevalence of thalassemia. Patients with thalassemia are thought to be at increased risk due to iron overload, splenectomy, and immune dysfunction. However, the pooled prevalence and mortality outcomes of melioidosis in this population remain unclear. Methods. We conducted a systematic review and meta-analysis in accordance with PRISMA 2020 guidelines (PROSPERO: CRD420251108294). PubMed, Embase, and Scopus were searched from inception to July 2025. Observational studies reporting prevalence or mortality of melioidosis in patients with thalassemia were eligible. Pooled odds ratios (ORs) for mortality were calculated using random-effects models, with subgroup and sensitivity analyses based on age, thalassemia subtype, and study quality. Results. Six retrospective studies including 7529 melioidosis patients, of whom 173 had thalassemia, were analyzed. The prevalence of thalassemia among melioidosis cases ranged from 0.5% to 40.7%. Mortality among thalassemia patients varied from 0% to 100%. Pooled analysis demonstrated no significant excess mortality compared with non-thalassemia controls (OR 0.55, 95% CI 0.16–1.89; I² = 44.9%). Sensitivity analysis restricted to moderate- and high-quality studies showed a significantly lower risk of death (OR 0.23, 95% CI 0.15–0.36; I² = 0%). Subgroup analyses by thalassemia subtype and age revealed no clear effect modification, although power was limited. Conclusions. Despite biological plausibility, thalassemia was not associated with increased melioidosis mortality. These findings suggest that closer clinical monitoring, iron chelation, and comorbidity profiles may influence outcomes. Prospective, well-characterized cohort studies are needed to refine risk stratification and guide management in endemic regions. Full article

Background/Objectives: Colorectal cancer (CRC) is a leading cause of cancer-related mortality worldwide. Iron metabolism and chronic inflammation are two interrelated processes that significantly influence the initiation and progression of CRC. Iron is essential for cell proliferation, but its excess promotes oxidative stress and DNA damage, while inflammation driven by cytokine-regulated pathways accelerates tumourigenesis. We therefore conducted this narrative review to collate the available evidence on the link between iron homeostasis and inflammatory signalling in CRC and highlight potential diagnostic and therapeutic applications. Methods: This narrative review of preclinical and clinical studies explores the molecular and cellular pathways that connect iron regulation and inflammation to CRC. Key regulatory molecules, such as the transferrin receptor (TFRC), ferroportin (SLC40A1), ferritin (FTH/FTL), hepcidin, and IL-6, were reviewed. Additionally, we summarised the findings of transcriptomic, epigenomic, and proteomic studies. Relevant therapeutic approaches, including iron chelation, ferroptosis induction, and anti-inflammatory strategies, were also discussed. Results: Evidence suggests that CRC cells exhibit altered iron metabolism, marked by the upregulation of transferrin receptor (TFRC), downregulation of ferroportin, and dysregulated expression of ferritin. Inflammatory mediators such as IL-6 activate hepcidin and STAT3 signalling, which reinforce intracellular iron retention and oxidative stress. Increased immune evasion, epithelial proliferation, and genomic instability appear to be linked to the interaction between inflammation and iron metabolism. Other promising biomarkers include ferritin, hepcidin, and composite gene expression signatures; however, their clinical application remains limited. Although several preclinical studies support the use of targeted iron therapies and combination approaches with anti-inflammatory agents or immunotherapy, there is a lack of comprehensive clinical validation confirming their efficacy and safety in humans. Conclusion: Although preclinical studies suggest that iron metabolism and inflammatory signalling form an interconnected axis closely linked to CRC, translating this pathway into reliable clinical biomarkers and effective therapeutic strategies remains a significant challenge. Future biomarker-guided clinical trials are essential to determine the clinical relevance and to establish precision medicine strategies targeting the iron–inflammation crosstalk in CRC. Full article

Although adding iron salts can improve phosphorus removal in membrane bioreactor (MBR) processes, overdosing iron salts may result in excessive iron concentrations in the effluent and pose risks of surface water contamination. In this study, an optimized iron salt dosing method was proposed to comprehensively investigate its effects on the performance of MBRs and the control of iron leakage. The results showed that batch dosing of solid iron salts (Fe₂(SO₄)₃) into the influent or activated sludge maintained an effluent Fe³⁺ concentration below 1.0 mg/L and a total phosphorus (TP) concentration below 0.30 mg/L. Long-term operation of the MBR (under conditions of HRT = 4.3 h, SRT = 20 d, and MLSS = 12 g/L) showed that batch dosing of solid iron salts led to an increase in the effluent ammonia–nitrogen (NH₃-N) concentration, and the nitrification effect was restored after supplementing the alkalinity. Iron salts increased the TP removal rate by approximately 40% while inhibiting the biological phosphorus removal capacity. The average Fe³⁺ concentration in the membrane effluent (0.23 ± 0.11 mg/L) met China’s Environmental Quality Standard for Surface Water (GB3838-2002). This study demonstrates that batch dosing of solid iron salts effectively controls iron concentration in the MBR effluent while preventing secondary pollution. The mechanisms of the impact of iron salts on MBR performance provide crucial theoretical and technical support for MBR process optimization. Full article

In plant tissues, nanoparticles can stimulate the production of reactive oxygen species (ROS), which, in excess, cause cellular toxicity by damaging membranes, chloroplasts, and DNA. However, they can also activate antioxidant mechanisms, aiding metabolic recovery under oxidative stress. In agriculture, iron oxide (nFe) nanoparticles stand out for their gradual release of the nutrient, preventing leaching and increasing productivity. This study aims to investigate whether iron oxide nanoparticles are effective alternatives for overcoming iron deficiencies, mitigating oxidative stress and restoring metabolic functions, while maintaining photosynthesis. The high H₂O₂ concentration observed in nFe 500 mg L⁻¹ (nFe 500) suggests that Fe, after being transported by the nanoparticles to the leaves, may have acted as a cofactor for antioxidant enzymes involved in H₂O₂ decomposition, reducing malondialdehyde concentration (MDA). Maintaining low oxidative stress suggests that H₂O₂ may function not only as a stress indicator but also as a signaling molecule in intracellular processes. nFe 500 suggests the ability of plants to utilize released Fe²⁺/Fe³⁺, restoring photosynthetic function in iron-deficient plants. Full article

The present study investigated the decline in human fertility by analyzing the multielemental profile of seminal plasma and its relationship with seminal parameters and sperm biomarkers. Twenty-nine donor seminal plasma samples were examined using inductively coupled plasma–tandem mass spectrometry (ICP-MS/MS). Method optimization demonstrated that robust plasma conditions, including internal standardization and helium (He) collision gas, were essential to achieve reliable quantification. These conditions mitigated matrix effects and spectroscopic interferences, despite lower sensitivity. Elements such as copper (Cu), iron (Fe), manganese (Mn), strontium (Sr), titanium (Ti), vanadium (V), and chromium (Cr) were quantified, and several significant correlations were identified. Specifically, Cu was negatively correlated with seminal volume and positively correlated with sperm concentration and spontaneous acrosome reacted sperm, but negatively correlated with medium mitochondrial membrane potential (MMP); Mn showed negative associations with sperm vitality and medium MMP; Fe showed a negative correlation with motile sperm concentration (4 h); V was positively correlated with acrosome reacted sperm after acrosome reaction induction and with very low/medium MMP, whereas it was negatively associated with tyrosine phosphorylation; and Cr also showed a negative correlation with tyrosine phosphorylation. As, Mo, and Pb were detected in a few samples, limiting correlation analysis. From a functional perspective, elements such as As and Pb, as well as excess Cu or Fe, may contribute to oxidative stress by enhancing reactive oxygen species (ROS) generation and impairing antioxidant defenses. Conversely, essential metals, including Mn and Cu, at physiological concentrations act as cofactors of antioxidant enzymes and play a protective role against oxidative damage. Full article