Search Results (212)

Green energy is expected to play an increasingly important role in the energy sector, so the volume of biogas production and the formation of anaerobic digestates is likely to increase in the future. A wide range of biodegradable organic materials are used in anaerobic digesters to produce biogas. This review focuses on the properties of anaerobic digestates and their effects on physical, chemical and biological soil parameters discussing the benefits, limitations and potential risks. Due to the variety of technologies and raw materials used, anaerobic digestates have diverse properties. Therefore, their impact on specific soil parameters, such as bulk density, aggregate stability, pH, electrical conductivity (EC), soil organic matter (SOM) or microbial activity can vary in magnitude and direction. These effects are also influenced by the variety of soils. Although digestates usually have a significant macro- and micronutrient content, their potentially toxic components or high salt content may limit their use. Despite the limitations, the application of anaerobic digestates generally has more advantages than disadvantages. The use of good-quality anaerobic digestates can improve the physical and chemical properties of the soil, increase soil nutrient and SOM content, as well as soil microbial activity. Full article

This study aimed to evaluate the dynamics of total carbon (TC), total nitrogen (TN), total phosphorus (TP), and some indicators of environmental sustainability of Nile tilapia reared in a biofloc technology (BFT) system. Nile tilapia fingerlings were cultured in three BFT units of production (4.2 m³ each) at a stocking density of 395 fish/m³. After 70 days, the survival rate was 98.05%, with a final average weight of 20.43 g, and apparent feed conversion of 1.05. Nutrient inputs were from supply water, initial fish biomass, and feed; outputs were measured from the final fish biomass and effluent. TC, TN, and TP concentrations in the water increased linearly over time (p < 0.001) by 1.54, 1.66, and 0.44 mg/L, respectively. Feed contributed over 88% of nutrient inputs, while fish final biomass accounted for over 50% of output. Nutrient retention in fish final biomass was 29.74% (TC), 45.38% (TN), and 46.34% (TP). The system had low eutrophication potential, estimated at 57.39 kg TC, 20.02 kg TN, and 5.70 kg TP per ton of fish. Water use was minimal (0.0074 m³/ton), but energy demand was high (114.59 MJ/kg). The closed system reduces biodiversity risks by preventing fish escape. In conclusion, BFT supports high fish productivity with efficient nutrient use, minimal water use, and limited environmental impact, contributing to environmentally sustainable aquaculture. Full article

Coal gangue is a fine-grained mineral with nutrient content, which can be used as a potential soil amendment. Nevertheless, current research on using coal gangue to improve soil water and support plant growth is still insufficient. In this study, coal gangue powder (CGP) was added to aeolian sandy soil. We compared the soil hydraulic properties and plant growth of original aeolian sandy soil (CK) and different CGP application rates (10% and 20%). The results indicated that the application of CGP transformed the soil texture from sandy to loamy, significantly reduced soil bulk density and saturated hydraulic conductivity (Ks) values, altered the soil water characteristic curve, enhanced soil water-holding capacity, and increased plant-available water. Compared with the CK group, the emergence rate of alfalfa seeds increased from approximately 50% to over 70% after CGP application. During the growth process, CGP application significantly elevated the net photosynthetic rate, transpiration rate, and stomatal conductance of alfalfa leaves. Rapid fluorescence kinetics monitoring of leaves demonstrated that alfalfa treated with CGP had a higher efficiency in light energy utilization. However, the photosynthetic capacity of leaves did not improve as the CGP application rate increased from 10% to 20%, suggesting that excessive CGP addition did not continuously benefit plant gas exchange. In conclusion, CGP application can improve the soil hydraulic properties of aeolian sandy soil and support plant growth and development, which is conducive to reducing the accumulated amount of coal gangue, alleviating plant water stress, and promoting ecological restoration in arid mining areas. We recommend a 10% addition of coal gangue powder as the optimal amount for similar soils. Full article

Heat stress (HS), an important environmental stressor for healthy poultry farming, has been shown to have a detrimental effect on production performance and induce serious diseases through immune system damage. As the avian peripheral immune system’s primary organ, spleen is subject to complex biological processes in response to HS injury. Histopathological characterization demonstrated that HS resulted in the destruction of the splenic red and white medulla, a decrease in cell density and organ atrophy. These changes directly impaired pathogen clearance and immune surveillance. At the physiological level, the impact of HS is characterized by disrupted metabolic homeostasis through interrupting neuroendocrine function. This, in turn, results in a significant suppression of humoral immune response. The oxidative-inflammatory cascade constitutes the core pathology of this disease. Energy metabolism disorder triggered by mitochondrial dysfunction and redox imbalance form a vicious circle, which promotes apoptosis signaling cascade. Meanwhile, over-activation of intrinsic immune system triggers a series of inflammatory factors, which further amplifies effects of tissue damage. The present prevention and control strategies are centered on synergistic anti-inflammatory and antioxidant interventions with nutrient modulators and plant actives. Nevertheless, it is imperative for future studies to incorporate multi-omics technologies in order to analyze the metabolic mechanisms and patterns of stress and establish a precise intervention strategy based on immune homeostatic regulation. This review systematically investigated the multilevel regulatory mechanisms of HS-induced spleen injury, which provides a theoretical basis for the mechanistic analysis and technological innovation of the prevention and control of HS syndrome in poultry. Full article

Rapid urbanization, climate variability, and land degradation are increasingly challenging traditional open-field farming systems. Soilless farming (SLF) has emerged as a complementary approach to enhance horticultural resilience in space-constrained and climate-stressed environments. This review critically evaluates the role of SLF within the broader framework of climate-smart agriculture (C-SA), with a particular focus on its applications in urban and peri-urban settings. Drawing on a systematic review of the existing literature, the study explores how SLF technologies contribute to efficient resource use, localized food production, and environmental sustainability. By decoupling crop cultivation from soil, SLF enables precise control over nutrient delivery and water use in enclosed environments, such as vertical farms, greenhouses, and container-based units. These systems offer notable advantages regarding water conservation, increased yield per unit area, and adaptability to non-arable or degraded land, making them particularly relevant for high-density cities, arid zones, and climate-sensitive regions. SLF systems are categorized into substrate-based (e.g., coco peat and rock wool) and water-based systems (e.g., hydroponics, aquaponics, and aeroponics), each with distinct design requirements, nutrient management strategies, and crop compatibility. Emerging technologies—including artificial intelligence, the Internet of Things, and automation—further enhance SLF system efficiency through real-time data monitoring and precision control. Despite these advancements, challenges remain. High setup costs, energy demands, and the need for technical expertise continue to limit large-scale adoption. While SLF is not a replacement for traditional agriculture, it offers a strategic supplement to bolster localized food systems and address climate-related risks in horticultural production. Urban horticulture is no longer a peripheral activity; it is becoming an integral element of sustainable urban development. SLF should be embedded within broader resilience strategies, tailored to specific socioeconomic and environmental contexts. Full article

Massive blooms of Ulva species, commonly known as green tides, pose serious ecological threats by disrupting coastal ecosystems and requiring costly removal efforts. This study presents a nature-based solution by seasonally valorizing Ulva ohnoi, a bloom-forming macroalga dominant in Jeju Island, South Korea. Biomass was collected across all four seasons and subjected to phylogenetic identification, biochemical characterization, and bioresource processing. Despite environmental fluctuations, tufA-based analysis confirmed U. ohnoi as the sole species present year-round. Carbohydrate content peaked in spring (55.35%) and was lowest in summer (45.74%), corresponding to maximum reducing sugar of 36.49 g/L in winter and 36.24 g/L in spring following acid-enzymatic hydrolysis. The maximum ethanol fermentation using Saccharomyces cerevisiae produced up to 17.12 g/L ethanol in spring with a yield of 0.47 g/g. Post-fermentation residues were enzymatically hydrolyzed into Ulva Ethanol Residue Medium (UERM), which supported yeast growth and fermentation comparable to commercial YPD medium, achieving final optical densities of 8.3–8.5 and ethanol production of 16.5–16.8 g/L. Alanine, valine, and proline were the most abundant amino acids in UERM, supporting its suitability as a nitrogen source. These findings highlight the potential of integrating green tide mitigation with renewable energy and nutrient recycling through seasonal, localized biorefineries aligned with circular marine bioeconomy principles. Full article

Blue maize is rich in bioactive compounds which are at risk of extinction due to monoculture practices. Carrot bagasse, considered a byproduct of the food industry, contains compounds that have been shown to benefit human health while also enhancing sustainability. Ellagic acid can prevent and assist in the treatment of various pathologies. Extrusion is a process characterized by its use of low energy, which minimizes the degradation of nutrients and bioactive compounds compared to other technologies. The objective of this research was to develop a functional food with high value of sensorial acceptance, desirable physicochemical, and antioxidant properties, using an 85:13:2% mixture of nixtamalized blue maize flour, carrot bagasse flour, and ellagic acid, processed with optimal conditions of extrusion determined with a surface response model. Operational conditions using a central rotatable experimental design were die temperature (DT = 120–170 °C), and screw speed (SS = 50–240 rpm), while response variables were physicochemical properties (expansion index, bulk density, breaking force, water absorption index and water solubility index) and antioxidant activity (free phenols inhibition of ABTS and DPPH). Sensory analysis, bromatological characterization and ellagic acid content determination with HPLC-DAD in reversed phase were also made. The optimal operational conditions were found to be DT = 144 °C and SS = 207 rpm, resulting in a mixture with high sensorial acceptability on a five-point hedonic scale. The optimized functional food may be used to promote the utilization of endemic ingredients and reduce food waste in the treatment of pathologies and prevention of diseases due to its high antioxidant activity attributed to phenolic and terpene compounds. Full article

Phytosterols (PSs) are widely present in plants, particularly abundant in plant oils and seeds. PSs are reported to have various biological activities, such as lowering cholesterol, alongside antioxidant and antibacterial activities. This research examined the effects of PSs in finishing pigs, including growth performance, serum biochemistry, and fecal bacteria. Two treatment groups (each treatment group consisted of five biological replicates, and each replicate comprised five pigs housed communally) were randomly assigned to the fifty finishing pigs (equally divided by sex) of “Duroc × Landrace × Yorkshire” three-way cross with 79.76 ± 1.29 (kg) body weight. The control group (CON) was given basic food, while the experimental group was given basic food containing 300 mg PS/kg (PS). Dietary PS supplementation markedly raised the levels of average daily feed intake (ADFI) and apparent digestibility of dry matter (DM), crude protein (CP), ether extract (EE), and gross energy (GE) in comparison to the CON (p < 0.05). Additionally, PSs also significantly boosted the concentrations of high-density lipoprotein cholesterol (HDL-C), total protein (TP), catalase (CAT), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), immunoglobulin G (IgG), motilin (MTL), and glucagon-like peptide-1 (GLP-1) (p < 0.05), and lowered the lactate dehydrogenase (LDH) level (p < 0.05). Both at the phyla and genus levels, the relative abundance of Firmicutes and Streptococcus increased significantly, and the relative abundance of Acinetobacter decreased significantly when adding phytosterols (p < 0.05). Overall, phytosterols dietary supplementation promotes immunity and antioxidant capacity in finishing pigs and boosts growth performance by improving nutrient digestibility. Full article

The rapid growth and high nutrient density in modern broiler production have led to issues like woody breast myopathy (WBM), footpad dermatitis, and fat accumulation, affecting welfare and profitability. This study evaluated the effects of amino acid (AA) and apparent metabolizable energy (AME) reductions on organ development, carcass yield, WBM incidence, and economic returns in Cobb 700 and Ross 708 broilers. Two trials were conducted, one per strain, using a factorial design with 12 treatments (four AA × three AME). Each trial included 864 broilers, randomly assigned to six replicate blocks, with 12 pens per block (six males and six females per pen). Diets contained 70%, 80%, 90%, or 100% of digestible AA and 84%, 92%, or 100% AME based on breeder recommendations. A 30% AA reduction increased fat pad weight, promoted proventriculus and jejunum development (day 58), reduced carcass and tenderloin weights, lowered moderate/severe WBM incidence (day 47), and shortened footpad dermatitis. A 16% AME reduction decreased fat pad weight, improved muscle production and returns, but reduced normal breast percentage (days 40 and 47). The recommended protein–energy ratio (g/MJ) for optimal economic returns was as follows: 19.78 (0–10 d), 17.51 (11–24 d), 16.03 (25–39 d), and 15.25 (40–63 d). Full article

Triticale (× Triticosecale Wittmack) is a valuable dual-purpose crop due to its adaptability to marginal environments and its potential for both high-quality grain and forage production. However, a comprehensive evaluation of its forage quality characteristics and agronomic performances is still needed. This study evaluated the grain and forage yield potentials and nutritional compositions of 11 triticale genotypes over two consecutive years in a semi-arid region located in Shanxi province, China. Forage quality was assessed using several key parameters, including nutrient composition, fiber digestibility, mineral content, and energy density, while grain quality parameters, including nutrient composition as well as carbohydrate and fiber characteristics, were also analyzed. Significant genetic variation was observed in these traits, indicating the influence of genotype–environment interactions on these traits. The tested genotypes exhibited grain yields ranging from 4.83 to 6.92 t ha⁻¹ and fresh forage biomass yields between 20.06 and 29.78 t ha⁻¹, demonstrating their potential for sustainable forage and grain production under semi-arid conditions. Genotypes from our breeding programs, including Shengnongsicao 1 and Jinsicao 1, demonstrated superior adaptability, maintaining stable forage and grain yield potentials under adverse conditions. Their favorable nutritional characteristics further enhance their suitability for semi-arid livestock systems. High levels of essential minerals, particularly calcium and potassium, further enhanced the nutritional value of these genotypes. These results provide valuable insights for triticale breeding programs and suggest triticale’s potential as a reliable crop in semi-arid regions, where maximizing land productivity is essential. Full article

Clostridium ljungdahlii is a model acetogenic bacterium utilized for ethanol production from syngas, with its growth and ethanol synthesis being profoundly influenced by fermentation pH. However, the mechanistic basis of this pH-dependent regulation remains poorly understood. In this study, we systematically investigated the impact of pH on the growth and metabolic profile of C. ljungdahlii under controlled pH conditions using CO as the sole carbon and energy source. At pH 6.0, C. ljungdahlii consumed around 6.0 M carbon monoxide, producing 413 ± 43 mM acetate, 288 ± 35 mM ethanol, and 17 ± 2 mM 2,3-butanediol, with a maximum optical density (OD) of 15.9. In contrast, at pH 5.3, the strain exhibited enhanced metabolic activity, consuming around 9.6 M carbon monoxide and generating 235 ± 24 mM acetate, 756 ± 26 mM ethanol, 38 ± 4 mM 2,3-butanediol, and 28 ± 7 mM lactate, achieving a maximum OD of 30. This represents an approximate twofold increase in both ethanol production and biomass accumulation compared to pH 6.0. Proteomic and parallel reaction monitoring (PRM) analyses demonstrated that the expression levels of key enzymes in central metabolic pathways were marginally higher at pH 6.0 than at pH 5.3, indicating that the observed physiological enhancements were not attributable to differential enzyme expression but likely stemmed from variations in ATP synthesis efficiency. Further optimization experiments revealed that the optimal pH for growth and ethanol production by C. ljungdahlii under CO-sufficient and nutrient-replete conditions is approximately 5.3. These findings provide critical insights into the pH-dependent metabolic regulation of C. ljungdahlii and establish essential parameters for scaling up syngas fermentation for ethanol production. Additionally, this study offers a foundation for further exploration of the unique proton motive force-driven ATP synthesis system in C. ljungdahlii and its broader implications for metabolic network regulation. Full article

The aim of this experiment is to investigate the effects of optimizing the dietary energy supply of Songliao Black growing and fattening pigs on their growth performance, nutrient digestibility, nitrogen balance, energy metabolism and oxidation energy supply, slaughter performance, and meat quality at a low ambient temperature. Forty-eight 75-day-old Songliao Black growing barrows with an initial weight of 33.38 ± 1.29 kg were randomized into two groups, with four replicates in each group and six pigs in each replicate. Two groups (CON group: low fat, normal energy; TES group: high fat, high energy) were fed isonitrogenous diets with different energy levels and fat contents. The experimental animals were raised at the same ambient temperature (10 ± 1 °C) all day. After 5 days of pre-feeding, the formal experiment began. Four Songliao Black barrows weighing approximately 80 kg were selected from each group for a five-day experimental period for digestibility and metabolism and respiratory calorimetry tests. All pigs (185 days of age) were slaughtered simultaneously at the end of the 110-day experimental period when their average body weight reached approximately 110 kg. The results showed that the average daily feed intake of the TES group was lower than that of the CON group (p < 0.05). Compared with the CON group, the feed-to-gain ratio was lower in the TES group during the fattening period (p < 0.05). Compared with the CON group, the crude fat digestibility, deposition energy, energy deposition rate, deposition energy of fat, and fat oxidation were higher (p < 0.05), and the intake and urinary nitrogen, carbohydrate oxidation, urinary energy, and protein oxidation were lower in the TES group (p < 0.05). Compared with the CON group, the serum high-density lipoprotein concentration, low-density lipoprotein concentration, and triglyceride concentration were higher in the TES group (p < 0.05), while alanine aminotransferase and aspartate aminotransferase concentrations were lower in the TES group (p < 0.05). Compared with the CON group, the backfat thickness was higher in the TES group (p < 0.05). Compared with the CON group, the weight gain/digestible protein and live lean meat mass/digestible protein were higher in the TES group (p < 0.05). Compared with the CON group, the yellowness (b*_45min) value of the longissimus thoracis was higher in the TES group (p < 0.05), and the shear force was lower (p < 0.05). Therefore, at a low ambient temperature, appropriately increasing the levels of dietary fat and energy was beneficial for improving the production performance and energy utilization efficiency and reducing CO₂ emissions and protein oxidation, saving protein resources of Songliao Black pigs. Full article

The main objective of this research was to observe the effects of feed restriction on caecum microbiota and metabolites in rabbits. Forty-eight male 8-week-old rabbits with similar body weights (1872.11 ± 180.85 g) were randomly assigned to two treatments according to completely randomized design: (1) the control group received ad libitum access to feed (AL), and (2) the treatment received 80% of the feed consumed by the control (FR). The results showed that FR did not differ (p > 0.05) for average daily weight gain or feed conversion ratio between the two groups. FR treatment led to a significant increase (p < 0.05) in acid detergent fibre apparent faecal digestibility, nitrogen digestibility and retention, and gross energy digestibility and retention. The FR treatment showed significantly (p < 0.05) lower blood triglycerides, creatinine, high-density lipoprotein cholesterol, malondialdehyde, and hydroxyl free radicals but significantly (p < 0.05) greater total antioxidant capacity and superoxide dismutase. The FR group presented greater (p < 0.05) Firmicutes and Ruminococcus abundances but a lower (p < 0.05) Akkermansiaceae abundance in the caecal content. Moreover, 222 differentiated metabolites were identified, and beta-alanine metabolism was the top enriched pathway. Collectively, FR can improve nutrient utilisation, lipid metabolism, antioxidant activity, caecum microbiota, and metabolites in rabbits. Full article

The fruit processing agroindustry generates waste, mainly composed of peels, which are often discarded but can be utilized as ingredients for developing new food products. However, their high perishability requires the application of preservation techniques, such as drying, which not only extends shelf life but also adds value and enables their conversion into flour, expanding their applications. This study evaluated the convective drying of pineapple peels for flour production, analyzing bioactive, physical, and thermal properties. Moisture was reduced by 91%, reaching a hygroscopic equilibrium of 6.84%. The Two-Term model provided the best fit for the data, with an R² above 0.9997. Effective diffusivity increased with temperature, ranging from 2.83 × 10⁻¹⁰ m²/s to 7.96 × 10⁻¹⁰ m²/s, with an activation energy of 47.90 kJ/mol, as described by the Arrhenius equation. Thermodynamic properties indicated an endothermic, non-spontaneous process, with reductions in enthalpy (45.21; 45.04 kJ/mol) and entropy (−0.2797; −0.2802 kJ/mol·K) and an increase in Gibbs free energy (135.60–141.20 kJ/mol) at higher temperatures. Fresh peels contained high levels of bioactive compounds, such as phenolics (1740.90 mg GAE/100 g d.b.) and tannins (613.42 mg TAE/100 g d.b.), which were best preserved at 70 °C. Drying altered the physical properties of the flour, resulting in higher absolute, apparent and compact densities, lower porosity (75.81%), and a reduced angle of repose (21.22°) suggesting greater material stability. Thermal analysis identified five mass loss events related to the degradation of water, carbohydrates, proteins, and fibers. Differential scanning calorimetry confirmed the thermal stability of the treatments. Thus, the study highlights pineapple peels as a promising raw material for producing nutrient-rich functional flour, with a drying temperature being a crucial factor in preserving bioactive compounds and achieving desirable product characteristics. Full article

Background/Objectives: The influence of individual differences in the selection of food portions can have a deep effect on recommendations for personalised nutrition. In addition to typical aspects such us energy density and nutrient composition, portion size is important for dietary recommendations. This study examined the dietary behaviours and portion size selection of 224 subjects in Spain and Germany to use such information to improve dietary adherence to a personalised nutrition app. Methods: An online questionnaire administered to adults in Spain and Germany collected sociodemographic data and dietary habits. The measurement of portion sizes was derived from a classification ranging from XXS to XL across 22 food groups, with assistance from a photographic atlas. Results: Significant differences across dimensions were found. Dietary habits showed that omnivores were the majority in both countries, with significant differences in the consumption of bread, desserts, and beverages. The Mediterranean diet was significantly followed by the Spanish group, reflecting cultural differences. Body mass index (BMI) was slightly higher among Germans, although both populations fell within the normal ranges. Portion size comparisons revealed statistically significant differences in the consumption of various food items between the two countries. Spaniards consumed higher amounts of rice, meat, and legumes, while Germans consumed larger portions of stews, lasagne, and pizza. These variations highlight differing dietary habits influenced by cultural preferences and dietary guidelines. Conclusions: The findings support the development of novel personalised nutrition apps that consider user preferences and enhance dietary adherence, thereby contributing to improved dietary recommendations and health outcomes. Full article