Search Results (286)

The timing of uranium mineralization in the Xiangshan ore field has long been controversial. Although various geochronometers have been applied by previous researchers, including pyrite Rb-Sr, mica Ar-Ar, and fluorite Sm-Nd, the results remain inconsistent and inconclusive. In recent years, the discovery of abundant Pb-Zn veins in the deeper parts of the Xiangshan ore field has further complicated the interpretation of its metallogenic history. In this study, abundant vein-type hydrothermal apatites closely associated with U-Pb-Zn polymetallic mineralization were identified in both uranium and Pb-Zn ore veins. Combined major-element Electron Probe Microprobe Analysis (EPMA), Laser Ablation-Inductively Coupled Plasma-Mass Spectrometry (LA-ICP-MS) U-Pb dating, and trace-element analysis were conducted on these apatite grains. The results suggest a mineralization age of 130.9 ± 1.1 Ma for the Shannan uranium deposit, which is consistent with the previously reported apatite U-Pb age of 131.3 ± 7.2 Ma from the Zoujiashan uranium deposit and coincides with the main pulse of volcanic-intrusive activity in the Xiangshan ore field (133–137 Ma). The deep Niutoushan Pb-Zn deposit suggests a younger mineralization age of 124.5 ± 1.3 Ma, which is consistent with a thermal event age of 125.6 Ma determined by zircon fission-track dating and the zircon LA-ICP-MS U-Pb age of late-stage granite porphyry (125.4 ± 1.0 Ma). These ages may constrain the timing of U-Pb-Zn polymetallic mineralization in the Xiangshan ore field. Both magmatic and hydrothermal apatites are classified as fluorapatite and exhibit similar chondrite-normalized rare earth element (REE) patterns. Compared with magmatic apatites, hydrothermal apatites are characterized by elevated Th, U, Ca, and Sr contents, depletion in light rare earth elements (LREEs), Mn, and Na, and distinctly lower Th/U ratios. On major-element variation diagrams, magmatic and hydrothermal apatites define coherent trends but display clear compositional differences related to their formation stages. Apatites from uranium ore veins show strongly negative Eu anomalies and weakly positive Ce anomalies, similar to magmatic apatites. In contrast, apatites from Pb-Zn ore veins display positive Eu anomalies and weakly negative Ce anomalies, with lower Mn and Ga contents and higher SO₃ contents relative to both magmatic apatites and hydrothermal apatites from uranium ore veins. These features indicate that the ore-forming fluids during Pb-Zn mineralization were characterized by significantly higher oxygen fugacity than those during uranium mineralization and magmatism. Combined with published Sr isotopic data for the Xiangshan ore field, we propose that both uranium and Pb-Zn mineralization were genetically linked to the prolonged magmatic evolution of the deep volcanic-intrusive complex. The subsequent incursion of meteoric water modified the physicochemical conditions of the ore-forming system, particularly during the formation of the Pb-Zn mineralization. Full article

The Yechangping super-large porphyry–skarn deposit is a key component of the East Qinling molybdenum metallogenic belt, central China. Magnetite is widely developed across all mineralization stages of this deposit, yet its systematic geochemical evolution and prospecting significance remain poorly constrained. This study presents in situ major- and trace-element analyses of magnetite via electron probe microanalysis (EPMA), laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS), and elemental mapping, to unravel the ore-forming hydrothermal evolution and establish reliable prospecting indicators. Four magnetite generations are identified based on petrography and paragenetic relationships: late skarn stage (Mt1), oxide stage (Mt2 and Mt3), and polymetallic sulfide stage (Mt4). Magnetite has total iron contents (TFeO, total Fe calculated as FeO) of 82.72–95.46 wt.% (values above the 93 wt.% stoichiometric limit of pure magnetite stem from minor oxidation), with dominant isovalent Fe³⁺ and Al³⁺ lattice substitution supported by a significant negative Fe–Al correlation. Systematic stage-dependent geochemical variations are observed: Mt1 has the highest Ti (mostly >1500 ppm), V and Cr, while Mt2–Mt4 show progressive Ti depletion (mostly <100 ppm), recording continuous cooling of the hydro-thermal system. V and Cr contents decrease markedly from Mt1 to Mt3, with secondary enrichment in Mt4; Mo concentrations peak in Mt2 (average 5.06 ppm), coupled with elevated chalcophile metalloid Te, As, Pb and Bi. Elemental mapping results show that K occurs as discrete hotspots, which may be mainly derived from feldspar microinclusions, rather than lattice substitution in magnetite. These geochemical fingerprints record a transition from high-temperature magmatic–hydrothermal fluids to late contact-metasomatic fluids, with evolving fluid–rock interaction and oxygen fugacity. Our results demonstrate that magnetite chemistry is a reliable tool for discriminating mineralization stages and vectoring prospecting targets in porphyry–skarn Mo–W systems. Full article

Background: Manganese (Mn) is an essential trace element with antioxidant properties; however, excessive exposure may contribute to inflammation and vascular dysfunction. Hair analysis provides an indicator of long-term Mn exposure. This study evaluated the relationship between hair Mn levels, acute coronary syndrome (ACS), coronary artery disease (CAD) severity, and cardiovascular risk factors, with particular emphasis on metabolic status in a cardiometabolic population. Methods: Hair Mn concentration was measured using inductively coupled plasma optical emission spectrometry (ICP-OES) in 80 patients (mean age 67 ± 11 years; 28.8% women) undergoing coronary angiography for suspected ACS. Final diagnoses included stable CAD (N = 42) and ACS [ST-elevation myocardial infarction (STEMI) N = 17, non-ST-elevation myocardial infarction (NSTEMI) N = 12, and unstable angina (UA) N = 9]. CAD severity was quantified using the SYNTAX score and the Coronary Artery Surgery Study Score (CASSS). Associations with clinical variables were assessed using non-parametric tests and Spearman correlations. The median SYNTAX score was 13.8 (range 0.0–68.5), and the median hair Mn concentration was 0.22 ppm (range 0.01–1.65). Results: SYNTAX scores were higher in ACS than in stable CAD (p = 0.027), with the highest values observed in NSTEMI. Hair Mn levels did not differ among diagnostic groups and showed no association with CASSS or SYNTAX (R = −0.11; p = 0.348). No differences were detected with respect to sex, smoking, prior myocardial infarction, hypertension, hyperlipidemia, or type 2 diabetes. A modest inverse correlation was observed between hair Mn and body mass index (BMI) in unadjusted analysis (R = −0.25; p = 0.03), but this association was not robust after correction for multiple comparisons, suggesting a potential exploratory link between manganese homeostasis and cardiometabolic status. Conclusions: Although hair Mn concentration was not associated with angiographic indices of CAD severity or ACS subtypes, the observed relationship with BMI may indicate a role of Mn homeostasis in cardiometabolic regulation. Larger prospective studies are required to clarify these associations. Full article

Apple pomace represents an important agro-industrial residue with high moisture content and significant environmental burden if improperly managed. This study investigated its thermochemical valorisation into biochar via two processes, followed by comprehensive physicochemical characterization and agronomic evaluation. Elemental analysis revealed carbon enrichment from 47.89% in raw material to 77–78% after the thermal process, evidencing a progressive aromatization. Scanning electron microscopy, Fourier transform infrared spectroscopy, and Raman analysis confirmed a temperature-dependent transition from partially amorphous carbon (400 °C) to more ordered aromatic structures (450 °C), while excessive thermal treatment (550 °C) increased structural defects. ICP-OES revealed an enrichment in thermally stable metals (Fe, Al, Mn) and limited Cd accumulation. Germination assays using Triticum aestivum L. demonstrated that biochar produced at 400 °C significantly improved the germination uniformity and seedling height (14.1 mm), as well as biomass accumulation compared to the control soil sample. The fertilizer addition increased the soluble Na and electrical conductivity (up to 643 µS/cm), potentially inducing transient salinity stress. Soil chemical analysis indicated increased K availability in soils amended with biochar produced at 400 °C, whereas the combination of biochar obtained at 450 °C with fertilizer conducted to elevated concentrations of certain trace metals, mainly Ni and Cr, highlighting the demand for careful monitoring. Overall, the biochar produced at 400 °C yielded to an optimal balance between structural stability, nutrient enrichment, and agronomic performance, evidencing that apple pomace may be a viable feedstock for sustainable biochar production within circular bioeconomy frameworks. Full article

The Xinqiao Cu-S polymetallic deposit is located in the Tongling ore concentration area of the Middle-Lower Yangtze River metallogenic belt. The orebodies consist of skarn orebodies and stratiform sulfide orebodies, but the genetic link between them remains controversial. In this study, magnetite was used as a proxy to systematically constrain the hydrothermal evolution from the intrusion to the contact zone and further to the stratiform orebodies. A representative drill hole (E603) was logged, and samples were systematically collected from the Jitou pluton outward to the contact zone. Composite samples from the 8–28 m interval were crushed and prepared as resin mounts for integrated TIMA automated mineralogy, BSE textural observation, and in situ LA-ICP-MS trace element analysis. Five types of magnetite (Mt1 to Mt5) were systematically identified. Mt1 occurs as inclusions within feldspar in the quartz monzodiorite. It exhibits typical magmatic magnetite characteristics and contains grid-like ilmenite exsolution, indicating crystallization during the late magmatic stage. Mt2 is distributed in the interstices of magmatic minerals, commonly showing hematitization and replacement of ilmenite exsolution lamellae by titanite. Its trace element geochemistry displays magmatic–hydrothermal transitional features. Mt3–Mt5 in the skarn and stratiform orebodies are paragenetic with retrograde alteration minerals (e.g., epidote, chlorite, and actinolite) and sulfides, and are characterized by low Ti, Al, and V contents and high Mg, Mn, and Sn contents, indicating a hydrothermal origin. From Mt3 to Mt5, (Ti + V) and (Al + Mn) decrease, while Zn and Mn increase, accompanied by a decrease in the (Si + Al)/(Mg + Mn) ratio. This reflects a trend of decreasing fluid temperature and progressively enhanced wall-rock buffering. The Mg-in-magnetite geothermometer yields relatively consistent results for Mt1–Mt3, but anomalously high temperatures for Mt4–Mt5. This suggests that the elevated Mg activity in the fluid, caused by reaction with carbonate wall rocks, can significantly influence the calculated temperatures. Therefore, this geothermometer should be used cautiously for magnetite in the outer skarn zone and interpreted in combination with other temperature constraints. The textures, paragenetic mineral assemblages, and trace element characteristics of magnetite collectively reveal a continuous mineralization process linking the skarn and stratiform orebodies at Xinqiao, providing robust mineralogical and geochemical evidence for the contribution of Yanshanian magmatic–hydrothermal activity to the stratiform mineralization. Full article

Railway corridors create persistent linear habitats embedded within intensively managed agricultural landscapes and can simultaneously support native biodiversity and facilitate the spread of undesirable taxa. We evaluated vegetation composition across five habitat types associated with railway line no. 250 (Havlíčkův Brod–Tišnov, Czech Republic): railway yard, railway embankment, railway land, field margin, and adjacent arable land. Vegetation was recorded using phytosociological relevés (10 m²) at four localities during three surveys in the 2021 growing season. In total, 83 plant taxa were identified, with pronounced differences among habitat types. Species richness and vegetation structure were highest in railway embankments, railway land, and field margins, whereas the railway yard and arable land exhibited lower diversity consistent with high disturbance intensity and substrate constraints. Canonical correspondence analysis distinguished habitat-affinity assemblages, indicating strong habitat filtering along the railway–agriculture gradient. Classification by origin and invasion status showed that non-native and invasive taxa were concentrated predominantly in railway embankments and adjacent habitats, suggesting elevated propagule pressure and potential spread into surrounding farmland. Colonization success (ICS) and colonization potential (ICP) indices indicated that railway-associated habitats can host taxa with high establishment capacity, contributing to successional stability within the corridor. These findings highlight railways as multifunctional elements of agricultural landscapes that require integrated vegetation management to balance biodiversity benefits with operational safety and invasive species risk. Full article

Background and Clinical Significance: Decompressive hemicraniectomy (DHC) is a life-saving intervention for malignant middle cerebral artery (MCA) infarction, but postoperative secondary injury mechanisms and functional outcome remain difficult to evaluate using intracranial pressure (ICP) alone. The pressure reactivity index (PRx), calculated as the moving correlation coefficient between ICP and mean arterial pressure (MAP), provides a measure of cerebral autoregulation. The utility of PRx monitoring in ischemic stroke, especially following DHC, remains uncertain. Case Presentation: We describe two patients presenting with acute ischemic stroke in the MCA territory who underwent DHC followed by postoperative ICP and PRx monitoring. Case 1 is a 40-year-old female with a left proximal MCA occlusion initially treated with endovascular thrombectomy (EVT) who required emergent DHC due to re-occlusion. Postoperatively, ICPs remained controlled, and PRx values were favorable (<0.2), indicating preserved cerebral autoregulation. She later showed moderate neurological improvement. Case 2 was a 68-year-old female with a left proximal MCA occlusion treated with EVT who developed worsening cerebral edema and midline shift, necessitating emergent DHC. Despite adequate ICP control, PRx values remained markedly elevated (0.45 to 0.73), consistent with impaired cerebral autoregulation, and her neurologic state remained poor at discharge. Conclusions: These contrasting cases suggest that PRx may provide physiologic information not reflected by ICP metrics alone post-DHC. PRx monitoring may provide complementary physiologic insight into postoperative autoregulatory status following DHC. Further investigation is warranted to define its role in individualized post-DHC management and prognostication in malignant ischemic stroke. Full article

Pegmatites with miarolitic cavities have not previously been reported from the Larsemann Hills, East Antarctica, and their age and origin remain poorly constrained. We report the first geochemical and geochronological data for fluorapatite from a newly discovered pegmatite with miarolitic cavities in the Larsemann Hills. Large Fe-rich fluorapatite crystals (up to 5 cm) contain abundant oriented monazite-(Ce) inclusions and display elevated REE (1397–7966 ppm), relatively high Y (945–4192 ppm), and low Sr (52.2–83.5 ppm). Their trace-element signatures plot within the fields of partial melts, high-grade metamorphic rocks, and evolved fluid-rich magmatic systems. U–Pb dating of fluorapatite yields concordant ages of 519 ± 4 Ma (ID-TIMS) and 521 ± 31 Ma (LA-ICP-MS), indicating crystallization during the D₄ stage of the Pan-African orogeny. The isotopic equilibrium between apatite and monazite inclusions suggests synchronous formation and late-stage fluid overprinting. Combined geological, geochemical, and isotopic evidence shows that the pegmatite formed in situ as a product of anatexis of the Broknes paragneisses and evolved within a volatile-rich magmatic–hydrothermal system. These results provide the first direct age constraints on pegmatites with miarolitic cavities in Antarctica and shed new light on the final stages of East Gondwana assembly. Full article

Herpes simplex virus type 2 (HSV-2) is the primary pathogen responsible for genital herpes. Predominantly transmitted via sexual contact, HSV-2 not only poses significant physical and psychological burdens on infected individuals but also substantially elevates the risk of HIV acquisition and represents a potentially fatal threat to newborns. Following primary infection, HSV-2 establishes lifelong latent infection within the sacral ganglia. Currently, there are no vaccines or therapeutics capable of eradicating this latent virus reservoir or effectively preventing initial infection. The core impediment to developing such interventions lies in the incomplete elucidation of the protective immune mechanisms against HSV-2 and its precise molecular pathogenesis. The host immune response against HSV-2 hinges critically on the coordinated interplay between innate and adaptive immunity. The innate immune system, serving as the first line of defense, acts to curtail early viral replication and initiate adaptive responses. This is achieved through mechanisms, including the genital mucosal barrier, activation of Toll-like receptors (TLRs), the cGAS-STING signaling pathway, interferon (IFN)-mediated antiviral effector functions, and activation of innate immune cells such as natural killer (NK) cells and dendritic cells (DCs). Crucially, however, HSV-2 counteracts these host defenses by expressing immune modulatory proteins (e.g., ICP0, ICP27, ICP35) that target key host antiviral signaling pathways, thereby affecting immune evasion. Within the adaptive immune response, neutralizing antibodies generated by the humoral immunity can provide localized protection at mucosal sites, but their protective efficacy is limited due to sophisticated viral immune evasion mechanisms. Cellular immunity, particularly mediated by CD4+ T cells, constitutes the core mechanism for viral clearance and suppression of recurrent outbreaks. Notably, tissue-resident memory T cells (TRMs) play a pivotal role in controlling the reactivation of latent HSV-2 within the ganglia. This review integrates current research advances to delineate the innate and adaptive immune mechanisms engaged during HSV-2 infection from the perspective of the dynamic host–virus interplay, with an ultimate aim to provide a theoretical foundation informing the rational development of preventive vaccines and therapeutic agents against HSV-2. Full article

The Guanfang tungsten deposit in the Bozhushan ore district, southeastern Yunnan, is genetically linked to Late Yanshanian granitic intrusions. To elucidate the petrogenesis and mineralization potential of the causative granite, this study presents a detailed mineral chemical analysis of biotite from the Guanfang pluton using electron probe microanalysis (EPMA) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The biotite crystals exhibit relatively high euhedrality, show no obvious alteration features, and are chemically characterized by reduced Na and Ca contents. These features, along with petrographic observations, confirm its origin as primary magmatic biotite. Crystallization conditions, calculated from biotite chemistry, indicate temperatures of 700–720 °C and pressures of 1.22–1.73 kbar, corresponding to a mesozonal emplacement depth of 4.6–6.5 km. Oxygen fugacity estimates, plotting near the Ni-NiO buffer, reveal an oxidized magmatic environment. Geochemical discrimination diagrams suggest the Guanfang granite exhibits transitional features between S-type and I-type affinities and is classified as a syn-melting (high-temperature) type. The biotite contains relatively low F (0.71–0.97 wt%), but elevated Cl (0.13–0.20 wt%) and Sn (43–56 µg/g) contents. This specific geochemical signature—combined with the medium- to high-temperature crystallization setting—is highly favorable for W-Sn mineralization. Furthermore, the high-Ti, syn-melting character of the granite implies additional potential for Cu-Pb-Zn-Au-Ag polymetallic mineralization. This study employs biotite chemistry to assess the petrogenesis and metallogenic potential of the Guanfang granite. The subsequent discovery of industrial ore bodies corresponding to some of the elements identified as having metallogenic potential confirms the feasibility of this approach. Accordingly, this method provides a new tool for future exploration in the Bozhushan district. Full article

Heavy metals in agricultural systems pose a significant challenge to food security, especially in regions with long-term intensive land use. While the Pannonian Plain represents Croatia’s primary breadbasket, accounting for a significant portion of the nation’s cereal production, data on the soil-to-grain transfer of heavy metals and the associated human exposure risk are limited. The objective of this study was (i) to determine the concentrations of arsenic (As), cadmium (Cd), and lead (Pb) in agricultural soils and corresponding grains (wheat, barley, and maize) across four principal counties within the Pannonian region of Croatia; (ii) to evaluate the soil-to-grain transfer factors that varied regionally and among cereal types; and (iii) to assess the potential non-carcinogenic health risks for both adults and children highlighting differences in exposure due to body weight and consumption patterns. Soil and cereal grain samples were collected in 2019 and 2020, and metal concentrations were determined by ICP-MS after microwave acid digestion. The transfer of metals from soil to grain was estimated using the transfer factor (TF), while exposure assessment was conducted by calculating the estimated daily intake (EDI), hazard quotient (HQ), and hazard index (HI). Due to the nonlinear distribution of the data and the lack of strictly matched soil and grain samples, median metal concentrations pooled across all studied regions were used for exposure assessment. For As, a conservative approach was applied, assuming that 50% of the total As is in inorganic form. Additionally, a probabilistic risk assessment using Monte Carlo simulations was conducted to account for variability in body weight and cereal intake, providing a more comprehensive evaluation of potential exposure. The results showed differences in metal accumulation among cereal species, with wheat and barley tending to accumulate more Cd than maize, while As and Pb concentrations in grains were low for all crops studied. Although soil metal concentrations in Međimurje County were generally low, elevated TF values for As and Pb were observed, indicating enhanced soil-to-plant transfer under specific local soil conditions. In contrast, high soil metal concentrations in Slavonski Brod–Posavina County were associated with low TF values, suggesting limited bioavailability and restricted transfer to cereal grains. Both deterministic and probabilistic assessments indicated that the HQ and HI for adults and children were below 1, suggesting low non-carcinogenic risk from cereal consumption. These findings highlight pronounced regional and crop-specific differences in soil-to-plant metal transfer and confirm that low soil contamination does not necessarily imply low transfer potential, emphasizing the importance of integrated soil–plant–grain monitoring for food safety assessment. Full article

Groundwater in Al-Madinah Al-Munawwarah faces considerable challenges from high salinity, elevated TDS, and nitrate contamination, primarily due to urbanization and industrial activities, making ongoing monitoring and management essential for its sustainable use in both drinking water and agriculture. The assessment of groundwater quality was conducted on 44 wells tapping two major aquifers (basaltic and alluvial) in the region, utilizing various geochemical techniques, including ICP-MS, FAAS, and XRF, to evaluate hydrochemical characteristics and identify the primary controlling factors. Key physicochemical parameters, including total dissolved solids (TDSs), electrical conductivity (EC), pH, total hardness (TH), and major ion concentrations, were evaluated. The results indicate that several parameters exceed permissible limits established by Gulf and international standards, reflecting highly saline conditions that could adversely affect drinking water safety and agricultural practices. Elevated nitrate levels and other contaminants indicate a combination of geological processes, including mineral leaching, and anthropogenic activities, such as agricultural runoff. Correlations among various ions reveal complex interactions driven by both natural and human factors. High nitrate and potassium concentrations, particularly in the alluvial aquifer, combined with weak correlations with geogenic ions, indicate anthropogenic inputs. Heavy metals in groundwater were classified into two groups: those within permissible limits (Ag, Ba, Be, Cd, Cr, Cu, Hg, Mn, Ni, Pb, Sb, and U) and those exceeding recommended limits (Zn, Al, As, Se, and Tl). Elevated metal concentrations are primarily attributed to water–rock interactions and the fertilizer use in surrounding agricultural areas. These findings highlight the urgent need for continuous monitoring and proactive groundwater to ensure sustainable and safe use of water resources. Full article

Coastal ecosystems are increasingly threatened by anthropogenic activities associated with tourism development and maritime traffic. This study evaluates the environmental quality of a coastal sector using an integrated approach combining sediment characteristics, heavy metal concentrations, and benthic foraminiferal assemblages. Nineteen surface sediments were collected and analyzed for trace metals using ICP-MS, while benthic foraminiferal assemblages were quantified, and ecological indices were calculated. Results reveal elevated concentrations of trace metals at coastal stations, closely associated with high TOM and fine-grained sediments, indicating significant anthropogenic inputs. These stations are characterized by low species richness, reduced Shannon diversity, high dominance, low living foraminiferal percentages, high malformed individuals, and markedly low FoRAM values, reflecting stressed environmental conditions. Opportunistic taxa such as Ammonia tepida dominate impacted sites, whereas sensitive carbonate-producing taxa (Quinqueloculina lamarckiana, Coscinospira hemprichii, Elphidium striatopunctatum, Elphidium crispum) prevail at less disturbed stations. Multivariate analyses clearly separate polluted coastal stations from relatively unimpacted offshore sites. The combined geochemical and biological evidence demonstrates that tourism-related activities and ship effluents exert a strong negative influence on benthic ecosystems. Benthic foraminifera, together with heavy metals, provide an effective and sensitive tool for assessing anthropogenic impacts and coral reef health for sustainable coastal management of Safaga Bay. Full article

Background: Both chronic kidney disease (CKD) and the haemodialysis procedure can contribute to disturbances in mineral homeostasis, which can potentially result in cellular pathologies. Our study aims to investigate trace element levels in haemodialysis patients and evaluate their potential impact on cellular adhesion molecules. This will clarify the clinical significance of trace element imbalances in this population. Methods: The study included 84 haemodialysis patients and 42 healthy controls. Trace element levels in blood (Zn, Cu, Mn, Mo, V, Sb and Cr) were measured using inductively coupled plasma mass spectrometry (ICP-MS), and cellular adhesion markers ICAM-1 and VCAM-1 were analysed by ELISA. Data analysis was conducted using SPSS 20.00, with significance set at p < 0.005. Results: Manganese (Mn) levels were significantly higher in haemodialysis patients (p = 0.019). Copper (Cu), Molybdenum (Mo), Vanadium (V), Antimony (Sb) and Chromium (Cr) levels were higher in the control group. Zinc (Zn) and Cr levels differed significantly between the control group (p = 0.018; p = 0.007). Cu levels were lower in hypertensive patients (p = 0.011), while Zn and Mn levels were higher in diabetic patients (p = 0.048 and p = 0.004, respectively). Dialysis duration, however, correlated with Sb (r = 0.295; p = 0.01), and Kt/V correlated with Mn, Sb and Cr (r = 0.256, r = 0.272 and r = 0.259, respectively; p = 0.05). Mo levels showed a positive correlation with both pre-dialysis (r = 0.230) and post-dialysis (r = 0.281) creatinine levels, and a negative correlation with post-dialysis GFR (r = −0.294). ICAM-1 and VCAM-1 levels were significantly elevated in dialysis patients (p = 0.001 for both); however, it was not found to be related to variables in the vascular access route. Conclusions: The levels of trace elements and adhesion molecules were examined in haemodialysis patients. High Mn levels indicate a risk of accumulation, while low Cu, Mo, V, Sb and Cr levels may require monitoring for deficiency. ICAM-1 and VCAM-1 levels in haemodialysis patients are associated with some trace elements (Mn and Zn); however, this relationship requires further evidence. In conclusion, the levels of trace elements and adhesion molecules in haemodialysis patients indicate the need for regular monitoring and show that the relationships between creatinine and GFR can be applied to larger patient groups. Full article

Background: Oral iron supplementation or food fortification is essential for managing or preventing iron deficiency but often causes gastrointestinal side effects. While solubility has traditionally been considered a requirement for iron uptake via the DMT1 transporter, recent evidence shows that insoluble iron can also be absorbed through endocytosis, raising questions about particle size and epithelial responses. Methods: Human intestinal cell lines (Hutu-80 and Caco-2) were exposed to physiologically relevant but elevated iron levels (0.5 mM Fe, 48 h) as ferric pyrophosphate, ferrous fumarate (both prone to precipitation), and soluble ferric EDTA. Cell survival and COX-2 protein were quantified by ELISA, solubility by ICP-OES, and particle size in cell culture medium by dynamic light scattering analyses. Results: Ferric pyrophosphate (0.62–3.8 μm) markedly increased COX-2 expression in Hutu-80 cells (254% ± 37%, n = 3, p = 4.11 × 10⁻⁵) and in Caco-2 cells (78% ± 8%, n = 3, p = 0.01) compared to the control. Ferrous fumarate (237–866 nm) also induced COX-2, but only in Hutu-80 cells (62% ± 11%, n = 3, p = 0.04), whereas ferric EDTA showed no effect in either cell line. COX-2 induction was associated with larger particles in the medium (≥237 nm), whereas smaller particles (<146 nm) were not. Conclusions: Particle size appears to be a critical determinant of cell survival and iron-induced epithelial COX-2 expression. Iron compounds that present as both soluble and particulate forms may optimize bioavailability, but controlling aggregate size (<146 nm) could reduce inflammatory signaling. These findings may have important implications for cell culture systems and warrant in vivo validation in iron supplemental studies. Full article