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Search Results (2,042)

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Keywords = electrical polarization

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27 pages, 2729 KiB  
Review
Polymer Composite-Based Triboelectric Nanogenerators: Recent Progress, Design Principles, and Future Perspectives
by Geon-Ju Choi, Sang-Hyun Sohn, Se-Jin Kim and Il-Kyu Park
Polymers 2025, 17(14), 1962; https://doi.org/10.3390/polym17141962 (registering DOI) - 17 Jul 2025
Abstract
The escalating consumption of fossil fuels and the rapid development of portable electronics have increased interest in alternative energy solutions that can sustainably self-power wearable devices. Triboelectric nanogenerators (TENGs), which convert mechanical energy into electricity through contact electrification and electrostatic induction, have emerged [...] Read more.
The escalating consumption of fossil fuels and the rapid development of portable electronics have increased interest in alternative energy solutions that can sustainably self-power wearable devices. Triboelectric nanogenerators (TENGs), which convert mechanical energy into electricity through contact electrification and electrostatic induction, have emerged as a promising technology due to their high voltage output, lightweight design, and simple fabrication. However, the performance of TENGs is often limited by a low surface charge density, inadequate dielectric properties, and poor charge retention of triboelectric materials. To address these challenges, recent research has focused on the use of polymer composites that incorporate various functional fillers. The filler materials play roles in improving dielectric performance and enhancing mechanical durability, thereby boosting triboelectric output even in harsh environments, while also diminishing charge loss. This review comprehensively examines the role of polymer composite design in TENG performance, with particular emphasis on materials categorized by their triboelectric polarity. Tribo-negative polymers, such as PDMS and PVDF, benefit from filler incorporation and phase engineering to enhance surface charge density and charge retention. By contrast, tribo-positive materials like nylon and cellulose have demonstrated notable improvements in mechanical robustness and environmental stability through composite strategies. The interplay between material selection, surface engineering, and filler design is highlighted as a critical path toward developing high-performance, self-powered TENG systems. Finally, this review discusses the current challenges and future opportunities for advancing TENG technology toward practical and scalable applications. Full article
(This article belongs to the Special Issue Advances in Polymer Composites for Nanogenerator Applications)
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17 pages, 3902 KiB  
Article
Electrical Potential-Induced Lubricity Changes in an Ionic Liquid-Lubricated Friction Pair
by Raimondas Kreivaitis, Audrius Žunda and Albinas Andriušis
Lubricants 2025, 13(7), 311; https://doi.org/10.3390/lubricants13070311 (registering DOI) - 17 Jul 2025
Abstract
The control of lubricity induced by electric potential is appealing for numerous applications. On the other hand, the high polarity of ionic liquids facilitates the adsorption of equally charged molecules onto polar surfaces. This phenomenon and its consequences are well understood at the [...] Read more.
The control of lubricity induced by electric potential is appealing for numerous applications. On the other hand, the high polarity of ionic liquids facilitates the adsorption of equally charged molecules onto polar surfaces. This phenomenon and its consequences are well understood at the nanoscale; however, they have recently garnered significant attention at the macroscale. This study investigates the lubricity of trihexyltetradecylphosphonium dicyanamide, a phosphonium ionic liquid, when used as a neat lubricant in reciprocating sliding under electrically charged conditions. Two different polarities with the same potential were applied to the friction pair of bearing steel against bearing steel while monitoring electrical contact resistance. The lubricity was evaluated through measurements of friction, wear, surface morphology, and composition. It was found that the application of electric potential significantly alters the lubricity of the investigated ionic liquid where a positive potential applied to the ball resulted in the least damaging situation. The recorded electrical contact resistance enabled the monitoring of tribofilm formation during reciprocation. It was found that there was minimal to no separation between interacting surfaces when the ball was changing direction. Full article
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14 pages, 8428 KiB  
Article
Spin-Orbit-Coupling-Governed Optical Absorption in Bilayer MoS2 via Strain, Twist, and Electric Field Engineering
by Lianmeng Yu, Yingliang Chen, Weibin Zhang, Peizhi Yang and Xiaobo Feng
Nanomaterials 2025, 15(14), 1100; https://doi.org/10.3390/nano15141100 - 16 Jul 2025
Abstract
This paper investigates strain-, twist-, and electric-field-tuned optical absorption in bilayer MoS2, emphasizing spin-orbit coupling (SOC). A continuum model reveals competing mechanisms: geometric perturbations (strain/twist) and Stark effects govern one-/two-photon absorption, with critical thresholds (~9% strain, ~2.13° twist) switching spin-independent to [...] Read more.
This paper investigates strain-, twist-, and electric-field-tuned optical absorption in bilayer MoS2, emphasizing spin-orbit coupling (SOC). A continuum model reveals competing mechanisms: geometric perturbations (strain/twist) and Stark effects govern one-/two-photon absorption, with critical thresholds (~9% strain, ~2.13° twist) switching spin-independent to spin-polarized regimes. Strain gradients and twist enhance nonlinear responses through symmetry-breaking effects while electric fields dynamically modulate absorption via band alignment tuning. By linking parameter configurations to absorption characteristics, this work provides a framework for designing tunable spin-resolved optoelectronic devices and advancing light–matter control in 2D materials. Full article
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35 pages, 10456 KiB  
Article
Amplified Westward SAPS Flows near Magnetic Midnight in the Vicinity of the Harang Region
by Ildiko Horvath and Brian C. Lovell
Atmosphere 2025, 16(7), 862; https://doi.org/10.3390/atmos16070862 - 15 Jul 2025
Viewed by 178
Abstract
Rare (only 10) observations, made in the southern topside ionosphere during 2015–2016, demonstrate the amplification of westward subauroral polarization streams (SAPS) up to 3000 m/s near the Harang region. The observed amplified SAPS flows were streaming antisunward after midnight and sunward at midnight, [...] Read more.
Rare (only 10) observations, made in the southern topside ionosphere during 2015–2016, demonstrate the amplification of westward subauroral polarization streams (SAPS) up to 3000 m/s near the Harang region. The observed amplified SAPS flows were streaming antisunward after midnight and sunward at midnight, where the dusk convection cell intruded dawnward. One SAPS event illustrates the elevated electron temperature (Te; ~5500 K) and the stable auroral red arc developed over Rothera. Three inner-magnetosphere SAPS events depict the Harang region’s earthward edge within the plasmasheet’s earthward edge, where the outward SAPS electric (E) field (within the downward Region 2 currents) and inward convection E field (within the upward Region 2 currents) converged. Under isotropic or weak anisotropic conditions, the hot zone was fueled by the interaction of auroral kilometric radiation waves and electron diamagnetic currents. Generated for the conjugate topside ionosphere, the SAMI3 simulations reproduced the westward SAPS flow in the deep electron density trough, where Te became elevated, and the dawnward-intruding westward convection flows. We conclude that the near-midnight westward SAPS flow became amplified because of the favorable conditions created near the Harang region by the convection E field reaching subauroral latitudes and the positive feedback mechanisms in the SAPS channel. Full article
(This article belongs to the Special Issue Feature Papers in Upper Atmosphere (2nd Edition))
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12 pages, 1298 KiB  
Article
Effect of Deuteration on the Temperature Dependence of the Quadratic Electro-Optic Effect in KDP Crystals
by Marek Izdebski and Rafał Ledzion
Materials 2025, 18(14), 3290; https://doi.org/10.3390/ma18143290 - 12 Jul 2025
Viewed by 182
Abstract
The results of precise measurements of the temperature dependencies of quadratic electro-optic coefficients, namely g1111g1122 and no3g1111ne3g3311, in KH2PO4 (KDP) and KD2PO4 [...] Read more.
The results of precise measurements of the temperature dependencies of quadratic electro-optic coefficients, namely g1111g1122 and no3g1111ne3g3311, in KH2PO4 (KDP) and KD2PO4 (DKDP) crystals at a wavelength of 632.8 nm are presented. We consider electro-optic coefficients describing changes in the optical impenetrability tensor resulting from an applied electric field, as well as intrinsic electro-optic coefficients defined in terms of induced polarization. The results show significant differences in the values of the analogous coefficients for the KDP and DKDP crystals and their temperature dependencies. Therefore, the quadratic electro-optic effect in KDP-type crystals cannot be easily described based solely on the contribution of PO4 tetrahedra, as assumed in current models of the linear effect. Moreover, the values of the intrinsic coefficients in the KDP and DKDP crystals differ even more than the corresponding usual electro-optic coefficients, which contradicts the conventional belief in their lower variability. Full article
(This article belongs to the Section Optical and Photonic Materials)
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10 pages, 1113 KiB  
Proceeding Paper
Examination of Nanochannels in Diluted Magnetic Doped CoTiSb Semiconductor
by Yuehua Chang
Eng. Proc. 2025, 98(1), 36; https://doi.org/10.3390/engproc2025098036 - 11 Jul 2025
Viewed by 74
Abstract
The first-principles calculation method was used to study doping elements with atomic numbers in the range of 23–30 (V–Zn) to form a single-atomic-spin nanochannel in a CoTiSb matrix. In a Ni-Sb single-atomic chain with high spin polarization and hole electrical conductivity, V-Sb, Mn-Sb, [...] Read more.
The first-principles calculation method was used to study doping elements with atomic numbers in the range of 23–30 (V–Zn) to form a single-atomic-spin nanochannel in a CoTiSb matrix. In a Ni-Sb single-atomic chain with high spin polarization and hole electrical conductivity, V-Sb, Mn-Sb, Fe-Sb, and Co-Sb single-atom chains have 100% spin polarization, indicating that a supercell containing the central atom chain has typical half-metal characteristics, and in the CoTiSb matrix, is centered on very small single-spin nanochannel forms. Using doping elements with atomic numbers between 23 and 27 (V-Co), the total magnetic moment of the supercell is constantly increasing, but the total magnetic moment of the Ni-doped supercell (Ni-Ti supercell) reduces, and a Cr-Ti supercell has an equal total magnetic moment. Doping elements Cu and Zn have atomic numbers higher than the range. Although the material of the nanochannel retains ferromagnetic properties, the spin polarization rate is reduced, and the material no longer has half-metallic properties. Full article
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12 pages, 3441 KiB  
Article
Mechanical Strength and Hydration Characteristic of Multiple Common Waste-Blended Cement-Based Materials Cured by Electric-Induced Heating Curing Under Severely Cold Environments
by Lei Zhang, Ruisen Li, Sheng Li, Han Wang and Qiang Fu
Materials 2025, 18(14), 3220; https://doi.org/10.3390/ma18143220 - 8 Jul 2025
Viewed by 246
Abstract
To address the challenges of concrete construction in polar regions, this study investigates the feasibility of fabricating cement-based materials under severely low temperatures using electric-induced heating curing methods. Cement mortars incorporating fly ash (FA-CM), ground granulated blast furnace slag (GGBS-CM), and metakaolin (MK-CM) [...] Read more.
To address the challenges of concrete construction in polar regions, this study investigates the feasibility of fabricating cement-based materials under severely low temperatures using electric-induced heating curing methods. Cement mortars incorporating fly ash (FA-CM), ground granulated blast furnace slag (GGBS-CM), and metakaolin (MK-CM) were cured at environmental temperatures of −20 °C, −40 °C, and −60 °C. The optimal carbon fiber (CF) contents were determined using the initial electric resistivity to ensure a consistent electric-induced heating curing process. The thermal profiles during curing were monitored, and mechanical strength development was systematically evaluated. Hydration characteristics were elucidated through thermogravimetric analysis (TG), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR) to identify phase compositions and reaction products. Results demonstrate that electric-induced heating effectively mitigates the adverse effect caused by the ultra-low temperature constraints, with distinct differences in the strength performance and hydration kinetics among supplementary cementitious materials. MK-CM exhibited superior early strength development with strength increasing rates above 10% compared to the Ref. specimen, which was attributed to the accelerated pozzolanic reactions. Microstructural analyses further verified the macroscopic strength test results that showed that electric-induced heating curing can effectively promote the performance development even under severely cold environments with a higher hydration degree and refined micro-pore structure. This work proposes a viable strategy for polar construction applications. Full article
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20 pages, 7140 KiB  
Article
Preparation of Carbon Fiber Electrodes Modified with Silver Nanoparticles by Electroplating Method
by Yuhang Wang, Rui Li, Tianyuan Hou, Zhenming Piao, Yanxin Lv, Changsheng Liu and Yi Xin
Materials 2025, 18(13), 3201; https://doi.org/10.3390/ma18133201 - 7 Jul 2025
Viewed by 277
Abstract
To solve the problems of carbon fiber (CF) electrodes, including poor frequency response and large potential drift, CFs were subjected to a roughening pretreatment process combining thermal oxidation and electrochemical anodic oxidation and then modified with Ag nanoparticles (AgNPs) using electroplating to prepare [...] Read more.
To solve the problems of carbon fiber (CF) electrodes, including poor frequency response and large potential drift, CFs were subjected to a roughening pretreatment process combining thermal oxidation and electrochemical anodic oxidation and then modified with Ag nanoparticles (AgNPs) using electroplating to prepare a CF electric field sensor. The surface morphology of the as-prepared AgNP-CF electric field sensor was characterized via optical microscopy, scanning electron microscopy, XPS, and energy-dispersive spectroscopy, and its impedance, polarization drift, self-noise, and temperature drift values were determined. Results show that the surface modification of the AgNP-CF electric field sensor is uniform, and its specific surface area is considerably increased. The electrode potential drift, characteristic impedance, self-noise, and temperature drift are 52.1 µV/24 h, 3.6 Ω, 2.993 nV/√Hz@1 Hz, and less than 70 µV/°C, respectively. Additionally, the AgNP-CF electric field sensor demonstrates low polarization and high stability. In field and simulated ocean tests, the AgNP-CF electrode exhibits excellent performance in the field and underwater environments, which renders it promising for the measurement of the ocean and geoelectric fields owing to its advantages, such as low noise and high stability. Full article
(This article belongs to the Section Advanced Nanomaterials and Nanotechnology)
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19 pages, 3961 KiB  
Article
Bernoulli Principle in Ferroelectrics
by Anna Razumnaya, Yuri Tikhonov, Dmitrii Naidenko, Ekaterina Linnik and Igor Lukyanchuk
Nanomaterials 2025, 15(13), 1049; https://doi.org/10.3390/nano15131049 - 6 Jul 2025
Viewed by 261
Abstract
Ferroelectric materials, characterized by spontaneous electric polarization, exhibit remarkable parallels with fluid dynamics, where polarization flux behaves similarly to fluid flow. Understanding polarization distribution in confined geometries at the nanoscale is crucial for both fundamental physics and technological applications. Here, we show that [...] Read more.
Ferroelectric materials, characterized by spontaneous electric polarization, exhibit remarkable parallels with fluid dynamics, where polarization flux behaves similarly to fluid flow. Understanding polarization distribution in confined geometries at the nanoscale is crucial for both fundamental physics and technological applications. Here, we show that the classical Bernoulli principle, which describes the conservation of the energy flux along velocity streamlines in a moving fluid, can be extended to the conservation of polarization flux in ferroelectric nanorods with varying cross-sectional areas. Geometric constrictions lead to an increase in polarization, resembling fluid acceleration in a narrowing pipe, while expansions cause a decrease. Beyond a critical expansion, phase separation occurs, giving rise to topological polarization structures such as polarization bubbles, curls and Hopfions. This effect extends to soft ferroelectrics, including ferroelectric nematic liquid crystals, where polarization flux conservation governs the formation of complex mesoscale states. Full article
(This article belongs to the Special Issue Research on Ferroelectric and Spintronic Nanoscale Materials)
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13 pages, 3092 KiB  
Article
Carbon Dioxide Gas Sensor Based on Terahertz Metasurface with Asymmetric Cross-Shaped Holes Empowered by Quasi-Bound States in the Continuum
by Kai He and Tian Ma
Sensors 2025, 25(13), 4178; https://doi.org/10.3390/s25134178 - 4 Jul 2025
Viewed by 255
Abstract
In this paper, a novel type of polarization-insensitive terahertz metal metasurface with cross-shaped holes is presented, which is designed based on the theory of bound states in continuous media. The fundamental unit of the metasurface comprises a metal tungsten sheet with a cross-shaped [...] Read more.
In this paper, a novel type of polarization-insensitive terahertz metal metasurface with cross-shaped holes is presented, which is designed based on the theory of bound states in continuous media. The fundamental unit of the metasurface comprises a metal tungsten sheet with a cross-shaped hole structure. A thorough analysis of the optical properties and the quasi-BIC response is conducted using the finite element method. Utilizing the symmetry-breaking theory, the symmetry of the metal metasurface is broken, allowing the excitation of double quasi-BIC resonance modes with a high quality factor and high sensitivity to be achieved. Analysis of the multipole power distribution diagram and the spatial distribution of the electric field at the two quasi-BIC resonances verifies that the two quasi-BIC resonances of the metasurface are excited by electric dipoles and electric quadrupoles, respectively. Further simulation analysis demonstrates that the refractive index sensitivities of the two quasi-BIC modes of the metasurface reach 404.5 GHz/RIU and 578.6 GHz/RIU, respectively. Finally, the functional material PHMB is introduced into the metasurface to achieve highly sensitive sensing and detection of CO2 gas concentrations. The proposed metallic metasurface structure exhibits significant advantages, including high sensitivity, ease of preparation, and a high Q-value, which renders it highly promising for a broad range of applications in the domains of terahertz biosensing and highly sensitive gas sensing. Full article
(This article belongs to the Special Issue The Advanced Flexible Electronic Devices: 2nd Edition)
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22 pages, 5129 KiB  
Article
A Dynamic Analysis of a Cantilever Piezoelectric Vibration Energy Harvester with Maximized Electric Polarization Due to the Optimal Shape of the Thickness for First Eigen Frequency
by Paulius Skėrys and Rimvydas Gaidys
Appl. Sci. 2025, 15(13), 7525; https://doi.org/10.3390/app15137525 - 4 Jul 2025
Viewed by 204
Abstract
This study presents an analytical and experimental approach to enhance cantilever-based piezoelectric energy harvesters by optimizing thickness distribution. Using a gradient projection algorithm within a state-space framework, the unimorph beam’s geometry is tailored while constraining the first natural frequency. The objective is to [...] Read more.
This study presents an analytical and experimental approach to enhance cantilever-based piezoelectric energy harvesters by optimizing thickness distribution. Using a gradient projection algorithm within a state-space framework, the unimorph beam’s geometry is tailored while constraining the first natural frequency. The objective is to amplify axial strain within the piezoelectric layers, thereby increasing electric polarization and maximizing the conversion efficiency of mechanical vibrations into electrical energy. The steady-state response under harmonic base excitation at resonance was modeled to evaluate the harvester’s dynamic behavior against uniform-thickness counterparts. Results show that the optimized beam achieves significantly higher output voltage and energy harvesting efficiency. Simulations reveal effective strain concentration in regions of high piezoelectric sensitivity, enhancing power generation under resonant conditions. Two independent experimental setups were employed for empirical validation: a non-contact laser vibrometry system (Polytec 3D) and a first resonant base excitation setup. Eigenfrequencies matched within 5% using a Polytec multipath interferometry system, and constant excitation tests showed approximately 30% higher in optimal shapes electrical potential value generation. The outcome of this study highlights the efficacy of geometric tailoring—specifically, non-linear thickness shaping—as a key strategy in achieving enhanced energy output from piezoelectric harvesters operating at their fundamental frequency. This work establishes a practical route for optimizing unimorph structures in real-world applications requiring efficient energy capture from low-frequency ambient vibrations. Full article
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14 pages, 2770 KiB  
Article
High-Energy Electron Emission Controlled by Initial Phase in Linearly Polarized Ultra-Intense Laser Fields
by Xinru Zhong, Yiwei Zhou and Youwei Tian
Appl. Sci. 2025, 15(13), 7453; https://doi.org/10.3390/app15137453 - 2 Jul 2025
Viewed by 259
Abstract
Extensive numerical simulations were performed in MATLAB R2020b based on the classical nonlinear Thomson scattering theory and single-electron model, to systematically examine the influence of initial phase in tightly focused linearly polarized laser pulses on the radiation characteristics of multi-energy-level electrons. Through our [...] Read more.
Extensive numerical simulations were performed in MATLAB R2020b based on the classical nonlinear Thomson scattering theory and single-electron model, to systematically examine the influence of initial phase in tightly focused linearly polarized laser pulses on the radiation characteristics of multi-energy-level electrons. Through our research, we have found that phase variation from 0 to 2π induces an angular bifurcation of peak radiation intensity, generating polarization-aligned symmetric lobes with azimuthal invariance. Furthermore, the bimodal polar angle decreases with the increase of the initial energy. This phase-controllable bimodal distribution provides a new solution for far-field beam shaping. Significantly, high-harmonic intensity demonstrates π-periodic phase-dependent modulation. Meanwhile, the time-domain pulse width also exhibits 2π-cycle modulation, which is synchronized with the laser electric field period. Notably, electron energy increase enhances laser pulse peak intensity while compressing its duration. The above findings demonstrate that the precise control of the driving laser’s initial phase enables effective manipulation of the radiation’s spatial characteristics. Full article
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19 pages, 937 KiB  
Review
Tissue Repair Mechanisms of Dental Pulp Stem Cells: A Comprehensive Review from Cutaneous Regeneration to Mucosal Healing
by Jihui He, Jiao Fu, Ruoxuan Wang, Xiaojing Liu, Juming Yao, Wenbo Xing, Xinxin Wang and Yan He
Curr. Issues Mol. Biol. 2025, 47(7), 509; https://doi.org/10.3390/cimb47070509 - 2 Jul 2025
Viewed by 375
Abstract
Repairing and regenerating tissue barriers is a key challenge in regenerative medicine. Stem cells play a crucial role in restoring the structural and functional integrity of key epithelial barrier surfaces, including the skin and mucosa. This review analyzes the role of dental pulp [...] Read more.
Repairing and regenerating tissue barriers is a key challenge in regenerative medicine. Stem cells play a crucial role in restoring the structural and functional integrity of key epithelial barrier surfaces, including the skin and mucosa. This review analyzes the role of dental pulp stem cells (DPSCs) and their derivatives, including extracellular vesicles, conditioned medium, and intracellular factors, in accelerating skin wound healing. The key mechanisms include: (1) DPSCs regulating inflammatory microenvironments by promoting anti-inflammatory M2 macrophage polarization; (2) DPSCs activating vascular endothelial growth factor (VEGF) to drive angiogenesis; (3) DPSCs optimizing extracellular matrix (ECM) spatial structure through matrix metalloproteinase/tissue inhibitor of metalloproteinase (MMP/TIMP) balance; and (4) DPSCs enhancing transforming growth factor-β (TGF-β) secretion to accelerate granulation tissue formation. Collectively, these processes promote wound healing. In addition, we explored potential factors that accelerate wound healing in DPSCs, such as oxidative stress, mechanical stimulation, hypertension, electrical stimulation, and organoid modeling. In addition to demonstrating the great potential of DPSCs for skin repair, this review explores their translational prospects in mucosal regenerative medicine. It covers the oral cavity, esophagus, colon, and fallopian tube. Some studies have found that combining DPSCs and their derivatives with drugs can significantly enhance their biological effects. By integrating insights from skin and mucosal models, this review offers novel ideas and strategies for treating chronic wounds, inflammatory bowel disease, and mucosal injuries. It also lays the foundation for connecting basic research results with clinical practice. This represents a significant step forward in tackling these complex medical challenges and lays a solid scientific foundation for developing more targeted and efficient regenerative therapies. Full article
(This article belongs to the Section Molecular Medicine)
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34 pages, 6837 KiB  
Article
Porcine Single-Eye Retinal Pigment Epithelium Cell Culture for Barrier and Polarity Studies
by Philipp Dörschmann, Sina von der Weppen, Emi Koyama, Johann Roider and Alexa Klettner
Cells 2025, 14(13), 1007; https://doi.org/10.3390/cells14131007 - 1 Jul 2025
Viewed by 382
Abstract
Age-related macular degeneration (AMD) is the main cause of blindness in Western nations. AMD models addressing specific pathological pathways are desired. Through this study, a best-practice protocol for polarized porcine single-eye retinal pigment epithelium (RPE) preparation for AMD-relevant models of RPE barrier and [...] Read more.
Age-related macular degeneration (AMD) is the main cause of blindness in Western nations. AMD models addressing specific pathological pathways are desired. Through this study, a best-practice protocol for polarized porcine single-eye retinal pigment epithelium (RPE) preparation for AMD-relevant models of RPE barrier and polarity is established. Single-eye porcine primary RPE cells (from one eye for one well) were prepared in 12-well plates including Transwell inserts. Different coatings (laminin (Lam), Poly-ᴅ-Lysine (PDL), fibronectin (Fn) and collagens) and varying serum contents (1%, 5% and 10%) were investigated to determine optimal culture parameters for this model. Success rates of cultures, cell number (trypan-blue exclusion assay), morphology/morphometry (light and fluorescence microscopy), protein secretion/expression (ELISA, Western blot), gene expression (qPCR), transepithelial electric resistance (TEER) and polar location of bestrophin 1 (BEST1) by cryosectioning (IHC-Fr) were assessed. Cells seeded on Lam exhibited the highest level of epithelial cells and confluence properties. Fn resulted in the highest cell number growth. Lam and Fn exhibited the highest culture success rates. TEER values and vascular endothelial growth factor secretion were highest when Lam was used. For the first time, polar (Transwell) porcine single-eye RPE morphometry parameters were determined. RPE on Lam showed bigger cells with a higher variety of cell shapes. CIV displayed the lowest claudin 19 expression. The highest basolateral expression of BEST1 was achieved with Lam coating. The higher the serum, the better the cell number increase and confluence success. A reduction in serum on Lam showed positive results for RPE morphology, while morphometry remained stable. A five percent serum on Lam showed the highest culture success rate and best barrier properties. RPE65 expression was reduced by using 10% serum. Altogether, the most suitable coating of Transwell inserts was Lam, and a reduction in serum to 5% is recommended, as well as a cultivation time of 28 days. A protocol for the use of polar porcine single-eye cultures with validated parameters was established and is provided herein. Full article
(This article belongs to the Special Issue Retinal Pigment Epithelium in Degenerative Retinal Diseases)
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13 pages, 3840 KiB  
Article
Second Harmonic Generation Imaging of Strain-Induced Domain Evolution Across Grain Boundaries in SrTiO3 Bicrystals
by Yuhang Ren and Piyali Maity
Surfaces 2025, 8(3), 47; https://doi.org/10.3390/surfaces8030047 - 1 Jul 2025
Viewed by 259
Abstract
Understanding strain behavior near grain boundaries is critical for controlling structural distortions and oxygen vacancy migration in perovskite oxides. However, conventional techniques often lack the spatial resolution needed to analyze phase and domain evolution at the nanoscale. In this paper, polarization-dependent second-harmonic generation [...] Read more.
Understanding strain behavior near grain boundaries is critical for controlling structural distortions and oxygen vacancy migration in perovskite oxides. However, conventional techniques often lack the spatial resolution needed to analyze phase and domain evolution at the nanoscale. In this paper, polarization-dependent second-harmonic generation (SHG) imaging is employed as a tool to probe local symmetry breaking and complex domain structures in the vicinity of a low-angle grain boundary of SrTiO3 (STO) bicrystals. We show that the anisotropic strain introduced by a tilted grain boundary produces strong local distortions, leading to the coexistence of tetragonal and rhombohedral domains. By analyzing SHG intensity and variations in the second-order nonlinear optical susceptibility, we map the distribution of strain fields and domain configurations near the boundary. In pristine samples, the grain boundary acts as a localized source of strain accumulation and symmetry breaking, while in samples subjected to intentional electrical stressing, the SHG response becomes broader and more uniform, suggesting strain relaxation. This work highlights SHG imaging as a powerful technique for visualizing grain-boundary-driven structural changes, with broad implications for the design of strain-engineered functional oxide devices. Full article
(This article belongs to the Collection Featured Articles for Surfaces)
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