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Search Results (2,873)

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22 pages, 4426 KiB  
Article
A Digital Twin Platform for Real-Time Intersection Traffic Monitoring, Performance Evaluation, and Calibration
by Abolfazl Afshari, Joyoung Lee and Dejan Besenski
Infrastructures 2025, 10(8), 204; https://doi.org/10.3390/infrastructures10080204 (registering DOI) - 4 Aug 2025
Abstract
Emerging transportation challenges necessitate cutting-edge technologies for real-time infrastructure and traffic monitoring. To create a dynamic digital twin for intersection monitoring, data gathering, performance assessment, and calibration of microsimulation software, this study presents a state-of-the-art platform that combines high-resolution LiDAR sensor data with [...] Read more.
Emerging transportation challenges necessitate cutting-edge technologies for real-time infrastructure and traffic monitoring. To create a dynamic digital twin for intersection monitoring, data gathering, performance assessment, and calibration of microsimulation software, this study presents a state-of-the-art platform that combines high-resolution LiDAR sensor data with VISSIM simulation software. Intending to track traffic flow and evaluate important factors, including congestion, delays, and lane configurations, the platform gathers and analyzes real-time data. The technology allows proactive actions to improve safety and reduce interruptions by utilizing the comprehensive information that LiDAR provides, such as vehicle trajectories, speed profiles, and lane changes. The digital twin technique offers unparalleled precision in traffic and infrastructure state monitoring by fusing real data streams with simulation-based performance analysis. The results show how the platform can transform real-time monitoring and open the door to data-driven decision-making, safer intersections, and more intelligent traffic data collection methods. Using the proposed platform, this study calibrated a VISSIM simulation network to optimize the driving behavior parameters in the software. This study addresses current issues in urban traffic management with real-time solutions, demonstrating the revolutionary impact of emerging technology in intelligent infrastructure monitoring. Full article
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32 pages, 1072 KiB  
Article
Machine Learning-Based Blockchain Technology for Secure V2X Communication: Open Challenges and Solutions
by Yonas Teweldemedhin Gebrezgiher, Sekione Reward Jeremiah, Xianjun Deng and Jong Hyuk Park
Sensors 2025, 25(15), 4793; https://doi.org/10.3390/s25154793 (registering DOI) - 4 Aug 2025
Abstract
Vehicle-to-everything (V2X) communication is a fundamental technology in the development of intelligent transportation systems, encompassing vehicle-to-vehicle (V2V), infrastructure (V2I), and pedestrian (V2P) communications. This technology enables connected and autonomous vehicles (CAVs) to interact with their surroundings, significantly enhancing road safety, traffic efficiency, and [...] Read more.
Vehicle-to-everything (V2X) communication is a fundamental technology in the development of intelligent transportation systems, encompassing vehicle-to-vehicle (V2V), infrastructure (V2I), and pedestrian (V2P) communications. This technology enables connected and autonomous vehicles (CAVs) to interact with their surroundings, significantly enhancing road safety, traffic efficiency, and driving comfort. However, as V2X communication becomes more widespread, it becomes a prime target for adversarial and persistent cyberattacks, posing significant threats to the security and privacy of CAVs. These challenges are compounded by the dynamic nature of vehicular networks and the stringent requirements for real-time data processing and decision-making. Much research is on using novel technologies such as machine learning, blockchain, and cryptography to secure V2X communications. Our survey highlights the security challenges faced by V2X communications and assesses current ML and blockchain-based solutions, revealing significant gaps and opportunities for improvement. Specifically, our survey focuses on studies integrating ML, blockchain, and multi-access edge computing (MEC) for low latency, robust, and dynamic security in V2X networks. Based on our findings, we outline a conceptual framework that synergizes ML, blockchain, and MEC to address some of the identified security challenges. This integrated framework demonstrates the potential for real-time anomaly detection, decentralized data sharing, and enhanced system scalability. The survey concludes by identifying future research directions and outlining the remaining challenges for securing V2X communications in the face of evolving threats. Full article
(This article belongs to the Section Vehicular Sensing)
13 pages, 7106 KiB  
Article
Multi-Scale Universal Style-Transfer Network Based on Diffusion Model
by Na Su, Jingtao Wang and Yun Pan
Algorithms 2025, 18(8), 481; https://doi.org/10.3390/a18080481 (registering DOI) - 4 Aug 2025
Abstract
Artistic style transfer aims to transfer the style of an artwork to a photograph while maintaining its original overall content. Although current style-transfer methods have achieved promising results when processing photorealistic images, they often struggle with brushstroke preservation in artworks, especially in styles [...] Read more.
Artistic style transfer aims to transfer the style of an artwork to a photograph while maintaining its original overall content. Although current style-transfer methods have achieved promising results when processing photorealistic images, they often struggle with brushstroke preservation in artworks, especially in styles such as oil painting and pointillism. In such cases, the extracted style and content features tend to include redundant information, leading to issues such as blurred edges and a loss of fine details in the transferred images. To address this problem, this paper proposes a multi-scale general style-transfer network based on diffusion models. The proposed network consists of a coarse style-transfer module and a refined style-transfer module. First, the coarse style-transfer module is designed to perform mainstream style-transfer tasks more efficiently by operating on downsampled images, enabling faster processing with satisfactory results. Next, to further enhance edge fidelity, a refined style-transfer module is introduced. This module utilizes a segmentation component to generate a mask of the main subject in the image and performs edge-aware refinement. This enhances the fusion between the subject’s edges and the target style while preserving more detailed features. To improve overall image quality and better integrate the style along the content boundaries, the output from the coarse module is upsampled by a factor of two and combined with the subject mask. With the assistance of ControlNet and Stable Diffusion, the model performs content-aware edge redrawing to enhance the overall visual quality of the stylized image. Compared with state-of-the-art style-transfer methods, the proposed model preserves more edge details and achieves more natural fusion between style and content. Full article
(This article belongs to the Section Evolutionary Algorithms and Machine Learning)
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31 pages, 2495 KiB  
Article
AIM-Net: A Resource-Efficient Self-Supervised Learning Model for Automated Red Spider Mite Severity Classification in Tea Cultivation
by Malathi Kanagarajan, Mohanasundaram Natarajan, Santhosh Rajendran, Parthasarathy Velusamy, Saravana Kumar Ganesan, Manikandan Bose, Ranjithkumar Sakthivel and Baskaran Stephen Inbaraj
AgriEngineering 2025, 7(8), 247; https://doi.org/10.3390/agriengineering7080247 - 1 Aug 2025
Viewed by 70
Abstract
Tea cultivation faces significant threats from red spider mite (RSM: Oligonychus coffeae) infestations, which reduce yields and economic viability in major tea-producing regions. Current automated detection methods rely on supervised deep learning models requiring extensive labeled data, limiting scalability for smallholder farmers. [...] Read more.
Tea cultivation faces significant threats from red spider mite (RSM: Oligonychus coffeae) infestations, which reduce yields and economic viability in major tea-producing regions. Current automated detection methods rely on supervised deep learning models requiring extensive labeled data, limiting scalability for smallholder farmers. This article proposes AIM-Net (AI-based Infestation Mapping Network) by evaluating SwAV (Swapping Assignments between Views), a self-supervised learning framework, for classifying RSM infestation severity (Mild, Moderate, Severe) using a geo-referenced, field-acquired dataset of RSM infested tea-leaves, Cam-RSM. The methodology combines SwAV pre-training on unlabeled data with fine-tuning on labeled subsets, employing multi-crop augmentation and online clustering to learn discriminative features without full supervision. Comparative analysis against a fully supervised ResNet-50 baseline utilized 5-fold cross-validation, assessing accuracy, F1-scores, and computational efficiency. Results demonstrate SwAV’s superiority, achieving 98.7% overall accuracy (vs. 92.1% for ResNet-50) and macro-average F1-scores of 98.3% across classes, with a 62% reduction in labeled data requirements. The model showed particular strength in Mild_RSM-class detection (F1-score: 98.5%) and computational efficiency, enabling deployment on edge devices. Statistical validation confirmed significant improvements (p < 0.001) over baseline approaches. These findings establish self-supervised learning as a transformative tool for precision pest management, offering resource-efficient solutions for early infestation detection while maintaining high accuracy. Full article
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30 pages, 59872 KiB  
Article
Advancing 3D Seismic Fault Identification with SwiftSeis-AWNet: A Lightweight Architecture Featuring Attention-Weighted Multi-Scale Semantics and Detail Infusion
by Ang Li, Rui Li, Yuhao Zhang, Shanyi Li, Yali Guo, Liyan Zhang and Yuqing Shi
Electronics 2025, 14(15), 3078; https://doi.org/10.3390/electronics14153078 - 31 Jul 2025
Viewed by 145
Abstract
The accurate identification of seismic faults, which serve as crucial fluid migration pathways in hydrocarbon reservoirs, is of paramount importance for reservoir characterization. Traditional interpretation is inefficient. It also struggles with complex geometries, failing to meet the current exploration demands. Deep learning boosts [...] Read more.
The accurate identification of seismic faults, which serve as crucial fluid migration pathways in hydrocarbon reservoirs, is of paramount importance for reservoir characterization. Traditional interpretation is inefficient. It also struggles with complex geometries, failing to meet the current exploration demands. Deep learning boosts fault identification significantly but struggles with edge accuracy and noise robustness. To overcome these limitations, this research introduces SwiftSeis-AWNet, a novel lightweight and high-precision network. The network is based on an optimized MedNeXt architecture for better fault edge detection. To address the noise from simple feature fusion, a Semantics and Detail Infusion (SDI) module is integrated. Since the Hadamard product in SDI can cause information loss, we engineer an Attention-Weighted Semantics and Detail Infusion (AWSDI) module that uses dynamic multi-scale feature fusion to preserve details. Validation on field seismic datasets from the Netherlands F3 and New Zealand Kerry blocks shows that SwiftSeis-AWNet mitigates challenges like the loss of small-scale fault features and misidentification of fault intersection zones, enhancing the accuracy and geological reliability of automated fault identification. Full article
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24 pages, 2325 KiB  
Review
Personalization of AI-Based Digital Twins to Optimize Adaptation in Industrial Design and Manufacturing—Review
by Izabela Rojek, Dariusz Mikołajewski, Ewa Dostatni, Jan Cybulski and Mirosław Kozielski
Appl. Sci. 2025, 15(15), 8525; https://doi.org/10.3390/app15158525 (registering DOI) - 31 Jul 2025
Viewed by 91
Abstract
The growing scale of big data and artificial intelligence (AI)-based models has heightened the urgency of developing real-time digital twins (DTs), particularly those capable of simulating personalized behavior in dynamic environments. In this study, we examine the personalization of AI-based digital twins (DTs), [...] Read more.
The growing scale of big data and artificial intelligence (AI)-based models has heightened the urgency of developing real-time digital twins (DTs), particularly those capable of simulating personalized behavior in dynamic environments. In this study, we examine the personalization of AI-based digital twins (DTs), with a focus on overcoming computational latencies that hinder real-time responses—especially in complex, large-scale systems and networks. We use bibliometric analysis to map current trends, prevailing themes, and technical challenges in this field. The key findings highlight the growing emphasis on scalable model architectures, multimodal data integration, and the use of high-performance computing platforms. While existing research has focused on model decomposition, structural optimization, and algorithmic integration, there remains a need for fast DT platforms that support diverse user requirements. This review synthesizes these insights to outline new directions for accelerating adaptation and enhancing personalization. By providing a structured overview of the current research landscape, this study contributes to a better understanding of how AI and edge computing can drive the development of the next generation of real-time personalized DTs. Full article
(This article belongs to the Section Computing and Artificial Intelligence)
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13 pages, 1879 KiB  
Article
Dynamic Graph Convolutional Network with Dilated Convolution for Epilepsy Seizure Detection
by Xiaoxiao Zhang, Chenyun Dai and Yao Guo
Bioengineering 2025, 12(8), 832; https://doi.org/10.3390/bioengineering12080832 (registering DOI) - 31 Jul 2025
Viewed by 147
Abstract
The electroencephalogram (EEG), widely used for measuring the brain’s electrophysiological activity, has been extensively applied in the automatic detection of epileptic seizures. However, several challenges remain unaddressed in prior studies on automated seizure detection: (1) Methods based on CNN and LSTM assume that [...] Read more.
The electroencephalogram (EEG), widely used for measuring the brain’s electrophysiological activity, has been extensively applied in the automatic detection of epileptic seizures. However, several challenges remain unaddressed in prior studies on automated seizure detection: (1) Methods based on CNN and LSTM assume that EEG signals follow a Euclidean structure; (2) Algorithms leveraging graph convolutional networks rely on adjacency matrices constructed with fixed edge weights or predefined connection rules. To address these limitations, we propose a novel algorithm: Dynamic Graph Convolutional Network with Dilated Convolution (DGDCN). By leveraging a spatiotemporal attention mechanism, the proposed model dynamically constructs a task-specific adjacency matrix, which guides the graph convolutional network (GCN) in capturing localized spatial and temporal dependencies among adjacent nodes. Furthermore, a dilated convolutional module is incorporated to expand the receptive field, thereby enabling the model to capture long-range temporal dependencies more effectively. The proposed seizure detection system is evaluated on the TUSZ dataset, achieving AUC values of 88.7% and 90.4% on 12-s and 60-s segments, respectively, demonstrating competitive performance compared to current state-of-the-art methods. Full article
(This article belongs to the Section Biosignal Processing)
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13 pages, 1132 KiB  
Review
M-Edge Spectroscopy of Transition Metals: Principles, Advances, and Applications
by Rishu Khurana and Cong Liu
Catalysts 2025, 15(8), 722; https://doi.org/10.3390/catal15080722 - 30 Jul 2025
Viewed by 305
Abstract
M-edge X-ray absorption spectroscopy (XAS), which probes 3p→3d transitions in first-row transition metals, provides detailed insights into oxidation states, spin-states, and local electronic structure with high element and orbital specificity. Operating in the extreme ultraviolet (XUV) region, this technique provides [...] Read more.
M-edge X-ray absorption spectroscopy (XAS), which probes 3p→3d transitions in first-row transition metals, provides detailed insights into oxidation states, spin-states, and local electronic structure with high element and orbital specificity. Operating in the extreme ultraviolet (XUV) region, this technique provides sharp multiplet-resolved features with high sensitivity to ligand field and covalency effects. Compared to K- and L-edge XAS, M-edge spectra exhibit significantly narrower full widths at half maximum (typically 0.3–0.5 eV versus >1 eV at the L-edge and >1.5–2 eV at the K-edge), owing to longer 3p core-hole lifetimes. M-edge measurements are also more surface-sensitive due to the lower photon energy range, making them particularly well-suited for probing thin films, interfaces, and surface-bound species. The advent of tabletop high-harmonic generation (HHG) sources has enabled femtosecond time-resolved M-edge measurements, allowing direct observation of ultrafast photoinduced processes such as charge transfer and spin crossover dynamics. This review presents an overview of the fundamental principles, experimental advances, and current theoretical approaches for interpreting M-edge spectra. We further discuss a range of applications in catalysis, materials science, and coordination chemistry, highlighting the technique’s growing impact and potential for future studies. Full article
(This article belongs to the Special Issue Spectroscopy in Modern Materials Science and Catalysis)
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35 pages, 4940 KiB  
Article
A Novel Lightweight Facial Expression Recognition Network Based on Deep Shallow Network Fusion and Attention Mechanism
by Qiaohe Yang, Yueshun He, Hongmao Chen, Youyong Wu and Zhihua Rao
Algorithms 2025, 18(8), 473; https://doi.org/10.3390/a18080473 - 30 Jul 2025
Viewed by 281
Abstract
Facial expression recognition (FER) is a critical research direction in artificial intelligence, which is widely used in intelligent interaction, medical diagnosis, security monitoring, and other domains. These applications highlight its considerable practical value and social significance. Face expression recognition models often need to [...] Read more.
Facial expression recognition (FER) is a critical research direction in artificial intelligence, which is widely used in intelligent interaction, medical diagnosis, security monitoring, and other domains. These applications highlight its considerable practical value and social significance. Face expression recognition models often need to run efficiently on mobile devices or edge devices, so the research on lightweight face expression recognition is particularly important. However, feature extraction and classification methods of lightweight convolutional neural network expression recognition algorithms mostly used at present are not specifically and fully optimized for the characteristics of facial expression images, yet fail to make full use of the feature information in face expression images. To address the lack of facial expression recognition models that are both lightweight and effectively optimized for expression-specific feature extraction, this study proposes a novel network design tailored to the characteristics of facial expressions. In this paper, we refer to the backbone architecture of MobileNet V2 network, and redesign LightExNet, a lightweight convolutional neural network based on the fusion of deep and shallow layers, attention mechanism, and joint loss function, according to the characteristics of the facial expression features. In the network architecture of LightExNet, firstly, deep and shallow features are fused in order to fully extract the shallow features in the original image, reduce the loss of information, alleviate the problem of gradient disappearance when the number of convolutional layers increases, and achieve the effect of multi-scale feature fusion. The MobileNet V2 architecture has also been streamlined to seamlessly integrate deep and shallow networks. Secondly, by combining the own characteristics of face expression features, a new channel and spatial attention mechanism is proposed to obtain the feature information of different expression regions as much as possible for encoding. Thus improve the accuracy of expression recognition effectively. Finally, the improved center loss function is superimposed to further improve the accuracy of face expression classification results, and corresponding measures are taken to significantly reduce the computational volume of the joint loss function. In this paper, LightExNet is tested on the three mainstream face expression datasets: Fer2013, CK+ and RAF-DB, respectively, and the experimental results show that LightExNet has 3.27 M Parameters and 298.27 M Flops, and the accuracy on the three datasets is 69.17%, 97.37%, and 85.97%, respectively. The comprehensive performance of LightExNet is better than the current mainstream lightweight expression recognition algorithms such as MobileNet V2, IE-DBN, Self-Cure Net, Improved MobileViT, MFN, Ada-CM, Parallel CNN(Convolutional Neural Network), etc. Experimental results confirm that LightExNet effectively improves recognition accuracy and computational efficiency while reducing energy consumption and enhancing deployment flexibility. These advantages underscore its strong potential for real-world applications in lightweight facial expression recognition. Full article
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26 pages, 5946 KiB  
Article
Flexural Strength of Cold-Formed Steel Unstiffened and Edge-Stiffened Hexagonal Perforated Channel Sections
by G. Beulah Gnana Ananthi, Dinesh Lakshmanan Chandramohan, Dhananjoy Mandal and Asraf Uzzaman
Buildings 2025, 15(15), 2679; https://doi.org/10.3390/buildings15152679 - 29 Jul 2025
Viewed by 174
Abstract
Cold-formed steel (CFS) channel beams are increasingly used as primary structural elements in modern construction due to their lightweight and high-strength characteristics. To accommodate building services, these members often feature perforations—typically circular and unstiffened—produced by punching. Recent studies indicate that adding edge stiffeners, [...] Read more.
Cold-formed steel (CFS) channel beams are increasingly used as primary structural elements in modern construction due to their lightweight and high-strength characteristics. To accommodate building services, these members often feature perforations—typically circular and unstiffened—produced by punching. Recent studies indicate that adding edge stiffeners, particularly around circular web openings, can improve flexural strength. Extending this idea, attention has shifted to hexagonal web perforations; however, limited research exists on the bending performance of hexagonal cold-formed steel channel beams (HCFSBs). This study presents a detailed nonlinear finite element (FE) analysis to evaluate and compare the flexural behaviour of HCFSBs with unstiffened (HUH) and edge-stiffened (HEH) hexagonal openings. The FE models were validated against experimental results and expanded to include a comprehensive parametric study with 810 simulations. Results show that HEH beams achieve, on average, a 10% increase in moment capacity compared to HUH beams. However, when evaluated using current Direct Strength Method (DSM) provisions, moment capacities were underestimated by up to 47%, particularly in cases governed by lateral–torsional or distortional buckling. A reliability analysis confirmed that the proposed design equations yield accurate and dependable strength predictions. Full article
(This article belongs to the Special Issue Cold-Formed Steel Structures)
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17 pages, 7301 KiB  
Article
Environmental Analysis for the Implementation of Underwater Paths on Sepultura Beach, Southern Brazil: The Case of Palythoa caribaeorum Bleaching Events at the Global Southern Limit of Species Distribution
by Rafael Schroeder, Lucas Gavazzoni, Carlos E. N. de Oliveira, Pedro H. M. L. Marques and Ewerton Wegner
Coasts 2025, 5(3), 26; https://doi.org/10.3390/coasts5030026 - 28 Jul 2025
Viewed by 167
Abstract
Recreational diving depends on healthy marine ecosystems, yet it can harm biodiversity through species displacement and habitat damage. Bombinhas, a biodiverse diving hotspot in southern Brazil, faces growing threats from human activity and climate change. This study assessed the ecological structure of Sepultura [...] Read more.
Recreational diving depends on healthy marine ecosystems, yet it can harm biodiversity through species displacement and habitat damage. Bombinhas, a biodiverse diving hotspot in southern Brazil, faces growing threats from human activity and climate change. This study assessed the ecological structure of Sepultura Beach (2018) for potential diving trails, comparing it with historical data from Porto Belo Island. Using visual censuses, transects, and photo-quadrats across six sampling campaigns, researchers documented 2419 organisms from five zoological groups, identifying 14 dominant species, including Haemulon aurolineatum and Diplodus argenteus. Cluster analysis revealed three ecological zones, with higher biodiversity at the site’s edges (Groups 1 and 3), but these areas also hosted endangered species like Epinephelus marginatus, complicating trail planning. A major concern was the widespread bleaching of the zoanthid Palythoa caribaeorum, a key ecosystem engineer, likely due to rising sea temperatures (+1.68 °C from 1961–2018) and declining chlorophyll-a levels post-2015. Comparisons with past data showed a 0.33 °C increase in species’ thermal preferences over 17 years, alongside lower trophic levels and greater ecological vulnerability, indicating tropicalization from the expanding Brazil Current. While Sepultura Beach’s biodiversity supports diving tourism, conservation efforts must address coral bleaching and endangered species protection. Long-term monitoring is crucial to track warming impacts, and adaptive management is needed for sustainable trail development. The study highlights the urgent need to balance ecotourism with climate resilience in subtropical marine ecosystems. Full article
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24 pages, 1008 KiB  
Article
Artificial Intelligence and Immersive Technologies: Virtual Assistants in AR/VR for Special Needs Learners
by Azza Mohamed, Rouhi Faisal, Ahmed Al-Gindy and Khaled Shaalan
Computers 2025, 14(8), 306; https://doi.org/10.3390/computers14080306 - 28 Jul 2025
Viewed by 285
Abstract
This article investigates the revolutionary potential of AI-powered virtual assistants in augmented reality (AR) and virtual reality (VR) environments, concentrating primarily on their impact on special needs schooling. We investigate the complex characteristics of these virtual assistants, the influential elements affecting their development [...] Read more.
This article investigates the revolutionary potential of AI-powered virtual assistants in augmented reality (AR) and virtual reality (VR) environments, concentrating primarily on their impact on special needs schooling. We investigate the complex characteristics of these virtual assistants, the influential elements affecting their development and implementation, and the joint efforts of educational institutions and technology developers, using a rigorous quantitative approach. Our research also looks at strategic initiatives aimed at effectively integrating AI into educational practices, addressing critical issues including infrastructure, teacher preparedness, equitable access, and ethical considerations. Our findings highlight the promise of AI technology, emphasizing the ability of AI-powered virtual assistants to provide individualized, immersive learning experiences adapted to the different needs of students with special needs. Furthermore, we find strong relationships between these virtual assistants’ features and deployment tactics and their subsequent impact on educational achievements. This study contributes to the increasing conversation on harnessing cutting-edge technology to improve educational results for all learners by synthesizing current research and employing a strong methodological framework. Our analysis not only highlights the promise of AI in increasing student engagement and comprehension but also emphasizes the importance of tackling ethical and infrastructure concerns to enable responsible and fair adoption. Full article
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30 pages, 2418 KiB  
Review
Combating Antimicrobial Resistance: Innovative Strategies Using Peptides, Nanotechnology, Phages, Quorum Sensing Interference, and CRISPR-Cas Systems
by Ana Cristina Jacobowski, Ana Paula Araújo Boleti, Maurício Vicente Cruz, Kristiane Fanti Del Pino Santos, Lucas Rannier Melo de Andrade, Breno Emanuel Farias Frihling, Ludovico Migliolo, Patrícia Maria Guedes Paiva, Paulo Eduardo Teodoro, Larissa Pereira Ribeiro Teodoro and Maria Lígia Rodrigues Macedo
Pharmaceuticals 2025, 18(8), 1119; https://doi.org/10.3390/ph18081119 - 27 Jul 2025
Viewed by 709
Abstract
Antimicrobial resistance (AMR) has emerged as one of the most pressing global health challenges of our time. Alarming projections of increasing mortality from resistant infections highlight the urgent need for innovative solutions. While many candidates have shown promise in preliminary studies, they often [...] Read more.
Antimicrobial resistance (AMR) has emerged as one of the most pressing global health challenges of our time. Alarming projections of increasing mortality from resistant infections highlight the urgent need for innovative solutions. While many candidates have shown promise in preliminary studies, they often encounter challenges in terms of efficacy and safety during clinical translation. This review examines cutting-edge approaches to combat AMR, with a focus on engineered antimicrobial peptides, functionalized nanoparticles, and advanced genomic therapies, including Clustered Regularly Interspaced Short Palindromic Repeats-associated proteins (CRISPR-Cas systems) and phage therapy. Recent advancements in these fields are critically analyzed, with a focus on their mechanisms of action, therapeutic potential, and current limitations. Emphasis is given to strategies targeting biofilm disruption and quorum sensing interference, which address key mechanisms of resistance. By synthesizing current knowledge, this work provides researchers with a comprehensive framework for developing next-generation antimicrobials, highlighting the most promising approaches for overcoming AMR through rational drug design and targeted therapies. Ultimately, this review aims to bridge the gap between experimental innovation and clinical application, providing valuable insights for developing effective and resistance-proof antimicrobial agents. Full article
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11 pages, 2805 KiB  
Article
A Novel CTC-Binding Probe: Enzymatic vs. Shear Stress-Based Detachment Approaches
by Sophia Krakowski, Sara Campos, Henri Wolff, Gabi Bondzio, Felix Hehnen, Michael Lommel, Ulrich Kertzscher and Paul Friedrich Geus
Diagnostics 2025, 15(15), 1876; https://doi.org/10.3390/diagnostics15151876 - 26 Jul 2025
Viewed by 286
Abstract
Background/Objectives: Liquid biopsy is a minimally invasive alternative to tissue biopsy and is used to obtain information about a disease from a blood sample or other body fluids. In the context of cancer, circulating tumor cells (CTC) can be used as biomarkers [...] Read more.
Background/Objectives: Liquid biopsy is a minimally invasive alternative to tissue biopsy and is used to obtain information about a disease from a blood sample or other body fluids. In the context of cancer, circulating tumor cells (CTC) can be used as biomarkers to determine the nature of the tumor, its stage of progression, and the efficiency of the administered therapy through monitoring. However, the low concentration of CTCs in blood (1–10 cells/mL) is a challenge for their isolation. Therefore, a minimally invasive medical device (BMProbe™) was developed that isolates CTCs via antigen–antibody binding directly from the bloodstream. Current investigations focus on the process of detaching bound cells from the BMProbe™ surface for cell cultivation and subsequent drug testing to enable personalized therapy planning. Methods: This article presents two approaches for detaching LNCaP cells from anti-EpCAM coated BMProbes™: enzymatic detachment using TrypLE™ and detachment through enzymatic pretreatment with supplementary flow-induced shear stress. The additional shear stress is intended to increase the detachment efficiency. To determine the flow rate required to gently detach the cells, a computational fluid dynamics (CFD) simulation was carried out. Results: The experimental test results demonstrate that 91% of the bound cells can be detached enzymatically within 10 min. Based on the simulation, a maximum flow rate of 47.76 mL/min was defined in the flow detachment system, causing an average shear stress of 8.4 Pa at the probe edges. The additional flow treatment did not increase the CTC detachment efficiency. Conclusions: It is feasible that the detachment efficiency can be further increased by a longer enzymatic incubation time or higher shear stress. The influence on the integrity and viability of cells must, however, be considered. Full article
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11 pages, 935 KiB  
Article
Rescue Blankets in Direct Exposure to Lightning Strikes—An Experimental Study
by Markus Isser, Wolfgang Lederer, Daniel Schwaiger, Mathias Maurer, Sandra Bauchinger and Stephan Pack
Coatings 2025, 15(8), 868; https://doi.org/10.3390/coatings15080868 - 23 Jul 2025
Viewed by 1033
Abstract
Lightning strikes pose a significant risk during outdoor activities. The connection between conventionally used rescue blankets in alpine emergencies and the risk of lightning injury is unclear. This experimental study investigated whether rescue blankets made of aluminum-coated polyethylene terephthalate increase the likelihood of [...] Read more.
Lightning strikes pose a significant risk during outdoor activities. The connection between conventionally used rescue blankets in alpine emergencies and the risk of lightning injury is unclear. This experimental study investigated whether rescue blankets made of aluminum-coated polyethylene terephthalate increase the likelihood of lightning injuries. High-voltage experiments of up to 2.5 MV were conducted in a controlled laboratory setting, exposing manikins to realistic lightning discharges. In a balanced test environment, two conventionally used brands were investigated. Upward leaders frequently formed on the edges along the fold lines of the foils and were significantly longer in crumpled rescue blankets (p = 0.004). When a lightning strike occurred, the thin metallic layer evaporated at the contact point without igniting the blanket or damaging the underlying plastic film. The blankets diverted surface currents and prevented current flow to the manikins, indicating potentially protective effects. The findings of this experimental study suggest that upward leaders rise from the edge areas of rescue blankets, although there is no increased risk for a direct strike. Rescue blankets may even provide partial protection against exposure to electrical charges. Full article
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