Search Results (372)

Contemporary muralism has gained increasing cultural and social relevance in recent years, becoming a prominent form of urban artistic expression. However, its outdoor exposure makes it highly vulnerable to environmental degradation, raising significant challenges for long-term preservation. While solar radiation is widely recognized as a main agent of deterioration, the impact of rainfall has received comparatively little attention. This study addresses this gap by evaluating the durability of commercial protective coatings applied to modern paints (alkyd, acrylic, and styrene-acrylic) under simulated rain exposure. The ageing protocol replicates approximately 10 years of cumulative rainfall in Central-Southern Europe. A key innovation of this research is the use of a custom-built rain chamber, uniquely designed to expose a large number of samples simultaneously under highly uniform and controlled rain conditions. The system ensures reproducible exposure through a precision-controlled moving platform and programmable rain delivery. A comprehensive set of analytical techniques was employed to assess morphological, chemical, and functional changes in the coatings and paints before and after ageing. Results highlight the limited performance of current protective materials and the need for more effective solutions for the conservation of contemporary outdoor artworks. Full article

Cystic fibrosis (CF), the most common hereditary lung disease in Caucasians, is caused by dysfunction of the cystic fibrosis transmembrane conductance regulator (CFTR). We evaluated CFTR function using a newly developed Ussing chamber system, the Multi Trans Epithelial Current Clamp (MTECC), in an in vitro model of human airway epithelia. Air–liquid interface (ALI) cultures were established from nasal brushings of healthy controls (HC) and CF patients with biallelic CFTR variants. ALI layer thickness was similar between groups (HC: 62 ± 13 µm; CF: 55 ± 9 µm). Immunofluorescence showed apical CFTR expression in HC, but reduced or absent signal in CF cultures. MTECC enabled continuous measurement of transepithelial resistance (Rt), potential difference (PD), and conductance (G_t). G_t was significantly reduced in CF cultures compared to HC (0.825 ± 0.024 vs. −0.054 ± 0.016 mS/cm²), indicating impaired cAMP-inducible ion transport by CFTR. Treatment of CF cultures with elexacaftor, tezacaftor, and ivacaftor (Trikafta^®) increased G_t, reflecting partial restoration of CFTR function. These findings demonstrate the utility of MTECC in detecting functional differences in CFTR activity and support its use as a platform for evaluating CFTR-modulating therapies. Our model may contribute to the development of personalized treatment strategies for CF patients. Full article

This study investigates the viability of a native Scenedesmus sp. strain for use in a 50 L bubble column photobioreactor designed to reduce greenhouse gas emissions under simulated spring, extreme summer, and winter conditions. The experiments were conducted by placing the reactor in a controlled climatic chamber, which allowed us to regulate the temperature, light intensity, and day–night cycles throughout the entire experiment. The results showed that under simulated spring conditions (a maximum temperature of 22 °C), the algal culture grew continuously for 61 days. Under extreme summer conditions (a maximum temperature of 39 °C), an initial drop in cell density was followed by recovery and continued growth over 75 days, although biomass production was 35% lower. Under winter conditions (a maximum temperature of 10 °C), the culture failed, indicating the need to prevent temperatures below 10 °C. In terms of biomass production, the culture densities achieved were 1.04 g L⁻¹ and 0.68 g L⁻¹ in the spring and summer trials, respectively. The Scenedesmus sp. strain demonstrated high carbon capture efficiency, tolerance to extreme heat, and sustained growth without the need for fresh medium or pH adjustments for over 60 days during spring and extreme summer conditions, confirming its potential for outdoor applications. Full article

True slime molds (Eumycetozoa) remain under-explored globally, particularly in water-logged forest habitats. Despite evidence suggesting a high biodiversity potential in the Knyszyn Forest of north-eastern Poland, no systematic effort had previously been undertaken there. In the present survey, plant substrates from eight swampy sub-compartments were incubated for over four months, resulting in the detection of fifteen slime mold species. Four of these taxa are newly reported for northern and north-eastern Poland, while several have been recorded only a handful of times in the global literature. These findings underscore how damp, nutrient-rich conditions foster Eumycetozoa and demonstrate the effectiveness of moist-chamber culturing in revealing rare or overlooked taxa. Current evidence shows that, although slime molds may occasionally colonize living plant or fungal tissues, their influence on crop productivity and tree vitality is negligible; they are therefore better regarded as biodiversity indicators than as pathogens or pests. By establishing a replicable framework for studying water-logged environments worldwide, this work highlights the ecological importance of swamp forests in sustaining microbial and slime mold diversity. Full article

Porcine endogenous retroviruses (PERVs) are integrated into the genome of all pigs. As they can be released as infectious virus particles capable of infecting human cells in vitro, they pose a potential risk for xenotransplantation involving pig cells or organs. To assess whether pigs produce infectious human-tropic viruses, infection assays with human cells are required. There are three main types of assays. First is the incubation of human target cells with gamma-irradiated pig cells. This method ensures that viral transmission is assessed in the absence of replicating pig cells. However, gamma irradiation may alter gene expression in pig cells, potentially affecting the results. Second is the co-culture in a double-chamber system in which pig and human cells are separated by a porous membrane, preventing direct cell-to-cell contact. While this method allows for the detection of infection by free virus particles, it does not account for infection via cell-to-cell transmission, which is a common mode of retroviral infection. And third is the co-culture of pig cells with human cells expressing a resistance gene. The resistance gene allows selective elimination of pig cells upon the addition of a selection medium. This assay enables both free virus and cell-to-cell transmission as well as complete removal of pig cells, which may not be fully achieved in the first type of assay. The third assay best simulates the conditions of in vivo xenotransplantation. However, in all cases the selection of donor and recipient cells is crucial to the experimental outcome. Results only indicate whether a specific pig cell type releases PERVs and whether a specific human cell type is susceptible to infection. A negative infection result does not necessarily reflect the in vivo situation, in which a transplanted organ consists of multiple pig cell types interacting with a diverse range of human cells within a living organism. Knowledge of these limitations is important for authorities regulating clinical applications for xenotransplantation. Full article

Livestock farming is a vital component of global food security, yet it remains a major contributor to greenhouse gas (GHG) emissions, particularly methane (CH₄), which has a global warming potential 28 times greater than carbon dioxide (CO₂). This review provides a comprehensive synthesis of current knowledge surrounding the sources, biological mechanisms, and mitigation strategies related to CH₄ emissions from ruminant livestock. We first explore the process of methanogenesis within the rumen, detailing the role of methanogenic archaea and the environmental factors influencing CH₄ production. A thorough assessment of both direct and indirect methods used to quantify CH₄ emissions is presented, including in vitro techniques (e.g., syringe method, batch culture, RUSITEC), in vivo techniques (e.g., respiration chambers, Greenfeed, laser CH₄ detectors), and statistical modeling approaches. The advantages and limitations of each method are critically analyzed in terms of accuracy, cost, feasibility, and applicability to different farming systems. We then examine a wide range of mitigation strategies, organized into four core pillars: (1) animal and feed management (e.g., genetic selection, pasture quality improvement), (2) diet formulation (e.g., feed additives such as oils, tannins, saponins, and seaweed), (3) rumen manipulation (e.g., probiotics, ionophores, defaunation, vaccination), and (4) manure management practices and policy-level interventions. These strategies are evaluated not only for their environmental impact but also for their economic and practical viability in diverse livestock systems. By integrating technological innovations with sustainable agricultural practices, this review highlights pathways to reduce CH₄ emissions while maintaining animal productivity. It aims to support decision-makers, researchers, and livestock producers in the global effort to transition toward climate-smart, low-emission livestock farming. Full article

Adaptive reuse of Mediterranean earthen houses offers a unique opportunity to fuse heritage preservation with sustainable development. This study introduces a comprehensive, sustainability-driven framework that reimagines these vernacular structures as culturally rooted and socially inclusive assets for contemporary living. Moving beyond conventional restoration, the proposed framework integrates environmental, socio-cultural, and economic sustainability across six core dimensions: ecological performance and material conservation, respectful functional transformation, structural resilience, cultural continuity and community engagement, adaptive flexibility, and long-term economic viability. Four geographically and culturally diverse case studies—Alhambra in Spain, Ghadames in Libya, the UCCTEA Chamber of Architects Main Building in North Cyprus, and Sheikh Hilal Beehive Houses in Syria—serve as testbeds to examine how earthen heritage can be reactivated in sustainable and context-sensitive ways. Through qualitative analysis, including architectural surveys, visual documentation, and secondary data, the study identifies both embedded sustainable qualities and persistent barriers, such as structural fragility, regulatory constraints, and socio-economic disconnects. By synthesizing theoretical knowledge with real-world applications, the proposed framework offers a replicable model for policymakers, architects, and conservationists aiming to bridge tradition and innovation. This research highlights adaptive reuse as a practical and impactful strategy for extending the life of heritage buildings, enhancing environmental performance, and supporting community-centered cultural regeneration across the Mediterranean region. Full article

The possibility of tightly controlling the cellular microenvironment within microfluidic devices represents an important step toward precision analysis of cellular phenotypes in vitro. Microfluidic platforms that allow both long-term mammalian cell culture and dynamic modulation of the culture environment can support quantitative studies of cells’ responses to drugs. Here, we report the design and testing of a novel microfluidic device of simple production (single Polydimethylsiloxane layer), which integrates a micromixer with vacuum-assisted cell loading for long-term mammalian cell culture and dynamic mixing of four different culture media. Finite element modeling was used to predict flow rates and device dimensions to achieve diffusion-based fluid mixing. The device showed efficient mixing and dynamic exchange of media in the cell-trapping chambers, and viability of mammalian cells cultured for long-term in the device. This work represents the first attempt to integrate single-layer microfluidic mixing devices with vacuum-assisted cell-loading systems for mammalian cell culture and dynamic stimulation. Full article

Constructing stable and flexible neuronal networks with multi-neurite wiring is essential for the in vitro modeling of brain function, connectivity, and neuroplasticity. However, most existing neuroengineering platforms rely on static microfabrication techniques, which limit the ability to dynamically control circuit architecture during cultivation. In this study, we developed a modifiable agarose gel-based platform that enables real-time microstructure fabrication using an infrared (IR) laser system under live-cell conditions. This approach allows for the stepwise construction of directional neurite paths, including sequential microchannel formation, cell chamber fabrication, and controlled neurite–neurite crossings. To support long-term neuronal health and network integrity in agarose microstructures, we incorporated direct glial co-culture into the system. A comparative analysis showed that co-culture significantly enhanced neuronal adhesion, neurite outgrowth, and survival over several weeks. The feeder layer configuration provided localized trophic support while maintaining a clear separation between glial and neuronal populations. Dynamic wiring experiments further confirmed the platform’s precision and compatibility. Neurites extended through newly fabricated channels and crossed pre-existing neurites without morphological damage, even when laser fabrication occurred after initial outgrowth. Time-lapse imaging showed a temporary growth cone stalling at crossing points, followed by successful elongation in all tested samples. Furthermore, the direct laser irradiation of extending neurites during microstructure modification did not visibly impair neurite elongation, suggesting minimal morphological damage under the applied conditions. However, potential effects on molecular signaling and electrophysiological function remain to be evaluated in future studies. Together, these findings establish a powerful, flexible system for constructive neuroengineering. The platform supports long-term culture, real-time modification, and multidirectional wiring, offering new opportunities for studying neural development, synaptic integration, and regeneration in vitro. Full article

This research explores the underappreciated traditional cold-water baths of Western Anatolia, once integral to the region’s agrarian culture. Due to waves of change, which had markedly begun by the pandemic in 2019 and the aftermath of the 2020 Samos earthquake, there has been a growing interest in living in peri-urban areas, resulting in the invasion of agricultural grounds by new construction, mainly including detached houses with gardens. Such a harsh growth not only threatens the fertile lands, but also the irreplaceable cultural heritage they embrace. In this regional frame, this study focuses on three surviving baths within the Karaburun Peninsula, casting light on their current precarious state as relics of a diminishing rural way of life and local heritage. The traditional cold-water baths, constructed amidst agricultural fields for seasonal use in select villages throughout İzmir, stand as unique exemplars of rural architecture. Characterised by their singular domed chambers and their reliance on water from adjacent wells, these structures today face abandonment and disrepair. Through a multi-disciplinary lens blending ethnography, oral history, and spatial analysis, this paper portrays these unassuming yet culturally impactful baths, elucidating their intrinsic value within the heritage domain. The inquiry contributes significantly to the heritage conservation discussion, highlighting the broad spectrum of values beyond mere historical interest. By articulating the symbiotic relationship between heritage and its community, this research underscores the pressing need to weave these baths into the fabric of current social structures, safeguarding their place within the collective memory. Full article

The Roman site at Makariopolsko village in Northeastern Bulgaria has been identified as a ceramic production center, featuring single- and double-chamber kilns, abundant ceramic material, and a nearby water source. Geological assessments also reveal local clay deposits. Previous archaeological studies have noted similar Roman production sites in the region, primarily focusing on the study of the kilns and the macroscopic description and classification of the ceramics. However, there has been a lack of research into the pottery’s composition and the sourcing of raw materials, which is essential for understanding the area’s cultural and economic context. This study aims to determine the raw material and firing temperature of the ceramic from the site at Makariopolsko village. Clay samples (both raw and fired at 1100 °C) and ceramic were subjected to chemical, statistical, phase X-ray structural, and thermal analyses. The findings indicate the use of calcareous illite–kaolinitic clay, sourced locally, with an added sandy component. The ceramics were fired at temperatures of 570–760 °C and 920–945 °C. These results, which support the site’s identification as a pottery production center, highlight advanced pottery skills and the dual functional capabilities of the kilns. Additionally, they pave the way for further research into regional production center relationships. Full article

Power operates across multiple scales, from physical action to complex social dynamics, and is constrained by fundamental principles. In the social realm, power is shaped by interactions and cognitive capacity: socially-facilitated empowerment enhances an agent’s information-processing ability, either by delegating tasks or leveraging collective resources. This computational advantage expands access to policies and buffers against vulnerabilities, amplifying an individual’s or group’s influence. In AIF, social power emerges from the capacity to attract attention and process information effectively. Our semantic habitat—narratives, ideologies, representations, etc.—functions through attentional scripts that coordinate social behavior. Shared scripts shape power dynamics by structuring collective attention. Speculative scripts serve as cognitive tools for low-risk learning, allowing agents to explore counterfactuals and refine predictive models. However, dominant scripts can reinforce misinformation, echo chambers, and power imbalances by directing collective attention toward self-reinforcing policies. We argue that power through scripts stems not only from associations with influential agents but also from the ability to efficiently process information, creating a feedback loop of increasing influence. This reframes power beyond traditional material and cultural dimensions, towards an informational and computational paradigm—what we term possibilistic power, i.e., the capacity to explore and shape future trajectories. Understanding these mechanisms has critical implications for political organization and technological foresight. Full article

This study aimed to evaluate the effect of the continuous use of the settling chamber for solids removal in the cultivation of the marine shrimp Penaeus vannamei and the halophyte Salicornia neei in an aquaponic system with bioflocs. Two treatments were tested: with settling and without settling. Each experimental unit consisted of an 800 L tank for shrimp rearing (stocking density of 375 shrimp m⁻³) and a hydroponic bench of 0.33 m² for 28 seedlings (84 plants m⁻²). In the treatment without settling, water was continuously pumped to the hydroponic bench. In the treatment with settling, the water was first pumped to the chamber, and the overflow was then distributed across each irrigation channel, returning to the tank by gravity. To maintain the concentration of suspended solids in the shrimp culture, solids that accumulated in the settling chamber were pumped back into the tank every 30 min. During the 54-day trial, the reduction in suspended solids in the treatment with settling led to an increase in TAN and NO₂ levels, while the concentration of NO₃ remained stable. Although water quality parameters were more stable in the treatment without settling, no significant differences were observed between the treatments regarding plant and shrimp production indices. These results demonstrate the feasibility of cultivating P. vannamei and S. neei in a biofloc-based aquaponic system without the continuous use of a settling chamber during the pre-grow phase (until 10 g), offering a potential method for simplifying aquaponic system design. Full article

This study examines the dynamics of information dissemination and opinion formation in Indonesian social media through a comprehensive analysis of a high-profile youth violence case. Using social network analysis (SNA), we analyzed 264,155 activities from 83,097 accounts on platform X (formerly Twitter) to understand the patterns of information flow, cluster formation, and inter-group interactions. The analysis revealed four distinct clusters with unique characteristics: a dominant support cluster (40.12%), a context-focused cluster (26.93%), a mainstream media cluster (14.14%), and a peripheral engagement cluster (6.05%). This study found significant patterns in information dissemination, with retweets dominating at 68% of total activities and strategic hashtag usage at 28%. Cross-cluster interactions comprised 20% of total activities, challenging assumptions about echo chambers in digital discourse. The network showed high resilience with 85% path reliability and demonstrated a consistent multiplier effect with a 1:5:15 ratio in message amplification. Bridge nodes (10–15% of accounts) played crucial roles in facilitating cross-cluster dialogue and maintaining network cohesion. The temporal evolution of discourse showed distinct phases, from initial factual reporting to later systemic analysis, with each phase characterized by different engagement patterns and narrative focuses. These findings extend existing theoretical frameworks while highlighting the need for more culturally nuanced approaches to understanding digital discourse in contexts of collectivist cultural dimensions. This study’s results have significant implications for digital literacy education, social media intervention strategies, and youth violence prevention efforts, suggesting the need for sophisticated, network-aware approaches that consider both structural dynamics and cultural contexts. Full article

The COVID-19 pandemic intensified concerns about misinformation, sparking interest in the field of infodemiology, which examines the spread and impact of information on public health perceptions. This research examines how geographic location influenced COVID-19 discourse across 10 Italian cities by analyzing geographically tagged Twitter data. Our network analysis of 4792 high-degree nodes identifies key information spreaders and community structures, while spatiotemporal mapping reveals regional variations in information patterns and influential narratives. Results demonstrate significant geographic and cultural influences on public discourse. In Milan and Rome, economic and political narratives dominated, suggesting targeted messaging about economic recovery and government transparency. Southern regions like Naples require trust-building through community-led initiatives addressing cultural health beliefs. The study identified a clear dichotomy among influencers: established public figures provided evidence-based information, while another group cultivated followings through conspiracy theories, creating echo chambers for skeptical views. This research informs strategies for location-specific information campaigns, helping public health agencies combat misinformation more effectively. Findings emphasize the need for context-specific interventions that consider geographic, cultural, and socioeconomic factors to enhance community resilience during health emergencies. Full article