Search Results (8,130)

Accurate detection and monitoring of antimicrobial resistance (AMR) in Helicobacter pylori mainly rely on phenotypic methods and culture, which can sometimes fail when bacterial load is low or after recent treatment. We investigated whether gastric biopsies classified as H. pylori-negative by standard diagnostic techniques still contain detectable bacterial DNA, including regions linked to AMR, and assessed whether selected DNA fragments can mediate allelic exchange in vitro. Gastric biopsies from 46 dyspeptic patients in the Democratic Republic of the Congo (including 23 phenotypically positive and 23 phenotypically negative individuals) were analyzed using long-read amplicon sequencing of seven resistance-associated loci, selective whole-genome amplification (sWGA) followed by long-read sequencing of H. pylori-enriched reads, and a proof-of-concept natural transformation assay. Phenotypically negative biopsies exhibited significantly lower sequencing depth across multiple loci (including 23S rRNA, gyrA, gyrB, and pbp1A; p = 0.003–0.014), indicating a reduced H. pylori DNA burden. However, AMR-associated mutations linked to various antibiotic classes were found in both groups. sWGA enabled recovery of fragmentary H. pylori sequence data from phenotypically negative samples, including reads that map to resistance- and virulence-associated genes. In vitro, 23S rRNA A2143G amplicons from both phenotypically positive and negative biopsies produced clarithromycin-resistant transformants in strain 26695. These findings indicate that phenotypically negative gastric biopsies might contain low-abundance and fragmentary H. pylori DNA. Although certain DNA fragments can mediate allelic exchange under controlled in vitro conditions, these results do not confirm bacterial viability, active infection, or clinically relevant in vivo resistance transfer. Therefore, they should be interpreted with caution in molecular AMR surveillance and detection contexts. Full article

Against the backdrop of the rapid development of generative artificial intelligence (GenAI), exploring preschool teachers’ willingness to adopt technology is critical for advancing their educational applications. However, this area remains underexplored. To address this gap, this study draws on the Unified Theory of Acceptance and Use of Technology (UTAUT) to develop a research model incorporating performance expectancy, effort expectancy, social influence, facilitating conditions, perceived risks, and tech-savviness. Using a sequential mixed-methods design, we recruited 434 teachers to participate in a GenAI teaching-application workshop, collected 399 valid questionnaires for structural equation modeling, and conducted 15 in-depth interviews. Quantitative results indicate that performance expectancy, social influence, and tech-savviness are positively associated with preschool teachers’ intention to use GenAI, while perceived risk is negatively associated; effort expectancy and facilitating conditions show no significant association. Due to methodological limitations including high inter-construct correlations and potential common method bias, these findings should be viewed as exploratory rather than conclusive. Qualitative interviews support these relationships and provide further explanatory insights. The mixed-methods results offer preliminary hypotheses regarding GenAI adoption among preschool teachers, and future confirmatory research is needed to verify their generalizability, especially in collectivist cultural contexts. Full article

The classic definitions of God and Trinity involving the concept of personhood are grounded in the lexical fields of ancient theological and philosophical discourse. They raise the question of the extent to which a believer is required to enter into those worldviews and conceptualities. If a semantic approach is adopted, it becomes apparent that a term like “person” may be re-accentuated according to context. This is an approach which is itself found within patristic methodology, not least in the transitions from Hellenistic philosophy to Greek Christian theology, and then from Greek to Latin. With this method in place, readers may approach such terms using materials derived from their own culture and context, not just those of antiquity. The Kiswahili proverb, Mtu ni watu (a person is people), provides an example of such a term, used to define personhood. Its adoption means that African Christians may approach a core doctrine of the Christian faith easily from their own cultural perspective without requiring a grounding in European thought and history, using a methodology already adopted by their Christian ancestor, Augustine of Hippo. Full article

Background/Objectives: The airway epithelium plays a central role in host defense, inflammatory signaling, and disease progression across infectious, inflammatory, and genetic respiratory disorders. Human nasal epithelial organoids have emerged as accessible and patient-specific in vitro platforms with increasing translational relevance. This systematic review aimed to critically evaluate the current evidence on nasal epithelial organoid models, focusing on donor characteristics, culture methodologies, differentiation strategies, and translational applications. Methods: A systematic search of PubMed/MEDLINE, Embase, Scopus, Ovid MEDLINE, and Cochrane Library was conducted for studies published between 1990 and April 2026. The review followed PRISMA guidelines and was structured according to the PICOTS framework. Eligible studies included in vitro experimental investigations using human-derived nasal epithelial organoids in infectious, inflammatory, or precision medicine contexts. Risk of bias was assessed using the QUIN tool. Results: Seventeen studies met the inclusion criteria. Applications clustered into three principal domains: infectious disease modeling, inflammatory and epithelial remodeling research, and cystic fibrosis precision medicine. Most studies employed expandable three-dimensional Matrigel-embedded organoids or organoid-derived air–liquid interface systems. Infection-focused studies demonstrated variant-specific viral replication dynamics and epithelial immune responses, while inflammatory models reproduced disease-associated differentiation and remodeling phenotypes. Cystic fibrosis oriented studies showed that organoid swelling and electrophysiological assays correlate with CFTR functional rescue and, in selected cases, clinical response. Methodological heterogeneity across protocols and outcome reporting precluded quantitative synthesis. Conclusions: Human nasal epithelial organoids represent versatile translational platforms bridging accessible patient-derived tissue and advanced airway disease modeling. Although variability in culture protocols and functional benchmarks limits standardization, these models hold significant promise for mechanistic investigation, therapeutic stratification, and precision medicine applications. Full article

This study investigates the role of the Massive Transformative Purpose (MTP) in shaping corporate brand identity and guiding brand management strategies in Exponential Organizations (ExOs). It examines how the MTP aligns internal and external brand dimensions, enhances stakeholder engagement, and drives societal impact, positioning it as a central element in ExO brand management. This study employs a qualitative multiple-case study methodology focusing on two ExOs: Airbnb, a digital-native hospitality company, and Mylia, a transformative learning enterprise. Semi-structured interviews with senior executives were triangulated with internal and external data to examine how the MTP drives strategy, culture, and stakeholder engagement. This allowed the application of the Corporate Brand Identity Matrix for exploring the different corporate brand identities and the relative nuances. The findings show that the MTP is essential to shaping ExOs’ corporate brand identity. It unifies organizational purpose, culture, and strategy, creating a cohesive identity that resonates both internally and externally. Embedding the MTP into daily practices fosters alignment, guides decision-making, strengthens stakeholder relationships, and shapes value propositions that distinguish ExOs while addressing stakeholder needs. The research bridges gaps in the literature on corporate brand identity, organizational purpose, and the unique characteristics of ExOs. It introduces the MTP Management Model, which integrates ExO-specific attributes to provide deeper insights into how these organizations align operational structures and brand identity with their transformative purpose. While the multiple-case study approach offers in-depth insights, the findings are context-specific and may not be fully generalizable across industries. The MTP Management Model provides a clear framework to integrate essential attributes, ensuring organizational coherence, effective communication, and enhanced competitiveness. Full article

Over the past two decades, research on issues of agency and liminality around borders has highlighted the mutual permeability, fluidity and overlapping of spheres such as religion and politics, providing arguments on the construction of identity and otherness that allow us to reappraise long-standing historical debates. This framework is particularly illuminating for the case of 15th-century Castile, when consolidation of a pioneering centralized monarchy in Europe witnessed the end of the coexistence between Christian, Jewish and Muslim communities, eventually leading to the persecution of converts and the expulsion of cultural and religious minorities. Drawing upon both primary and secondary sources, and adopting the analytical framework of frontier-crossing, this article identifies the conditions under which particular social agents reconfigured the boundaries between religion and politics in 15th-century Castile. It further examines the process by which border crossing by various agents made customs and everyday practices crystallize into a third sphere for the construction of alterity and exclusion and analyzes the specific context in which the intersection of these three domains contributed to the stigmatization of Jews, Muslims and converts, ultimately leading to their exclusion and expulsion. Initially subordinated to theological and legal concerns, social practices, rituals and ceremonies became central to discourse intersecting the political, religious and moral domains, underpinning social stigmatization and the institutional mechanisms of rising monarchical centralization. Full article

Tourism education is central to human capital development under Saudi Arabia’s Vision 2030, yet the extent to which curricula align with emerging industry requirements remains underexplored, particularly in developing economy contexts. This qualitative study examines student and faculty perspectives on curriculum alignment at the University of Ha’il’s Tourism and Antiquities Department. Twenty participants were purposively recruited and interviewed; data were analyzed using thematic analysis. Findings reveal four interconnected challenges: a persistent theory–practice gap sustained by lecture-based pedagogies, insufficient integration of digital and smart tourism technologies, weak industry–academia partnerships, and structural barriers to interdisciplinary collaboration. In response, this study proposes an interdisciplinary integration model structured around five domains: digital technology, sustainability and environment, business and entrepreneurship, cultural and creative industries, and social sciences and community engagement. The model provides a progressive framework for cross-departmental collaboration and represents the study’s primary practical contribution. Theoretically, the study demonstrates that curriculum misalignment operates through mutually reinforcing institutional constraints rather than discrete correctable deficits. Recommendations address curriculum reform, technology investment, structured partnership development, and administrative conditions enabling interdisciplinary implementation. Full article

This paper examines the influence of Aldous Huxley’s dystopian vision—particularly Brave New World—on Ali Smith’s Seasonal Quartet, arguing that Smith’s post-Brexit novels can be read as contemporary, politically embedded responses to the dystopian tradition Huxley helped establish. While Smith’s fiction is rarely labelled dystopian in genre, the Quartet is deeply informed by a dystopic sense of cultural, ecological, and political decay in 21st-century Britain. I propose that Smith adopts and adapts key dystopian motifs from Huxley but repurposes them through a radical humanist lens that privileges relationality, art, and memory as sources of resistance and repair. The paper will be structured in three sections. The first outlines Huxley’s dystopian framework, with a focus on Brave New World’s criticism of technological control, emotional appeasement, and the suppression of dissent through pleasure. The second analyzes Smith’s Seasonal Quartet as a world not governed by totalitarian regimes but by apathy, misinformation, and ideological fragmentation. The final section traces Smith’s divergence from Huxley: where Huxley’s world often excludes hope in favor of bleak satire, Smith inserts gestures of resistance, particularly through intergenerational friendships, the presence of art and literature, and the recurrence of seasonal cycles as metaphors for renewal. Although Autumn explicitly references Huxley’s Brave New World, sustained critical comparisons between the two authors remain relatively rare. Most scholarship approaches Huxley through the tradition of twentieth-century dystopian fiction, while Smith’s Quartet is typically discussed within the context of Brexit literature and contemporary narrative experimentation. Reading the Quartet alongside Huxley, therefore, reveals an unexpected dialogue between early twentieth-century dystopian critique and twenty-first-century literary responses to political crisis. Full article

The construction sector accounts for approximately 36% of global final energy consumption and close to 40% of total CO₂ emissions, making it a primary target of international climate policy. Despite this growing attention, the indigenous building traditions of the Ecuadorian Andes remain virtually absent from the international scientific literature on vernacular sustainability. This study presents a systematic field documentation and bioclimatic assessment of vernacular bahareque dwellings in the Kichwa-Saraguro community of Ilincho, canton of Saraguro, province of Loja, Ecuador (2700 m a.s.l.). A field survey of 30 dwellings identified five morphological typologies—I-1P, I-2P, 2B, L, and C—with typology C, a compact C-shaped block with a three-sided portal, accounting for 53.3% of the sample. A structured multi-criteria framework of 48 bioclimatic indicators distributed across eight categories, adapted to the cold-temperate mountain climate of the study area, was applied to quantify each typology’s bioclimatic performance. All typologies exceeded 75% overall compliance on the global Bioclimatic Performance Index (BPI), with typology C achieving the highest value (88.5%). Categories F (Materials and construction) and H (Cultural and social aspects) scored 100% across all typologies, reflecting system-level properties of the bahareque constructive system rather than morphological differences between typological variants; a supplementary morphological BPI restricted to Categories A–E and G is reported. An exploratory, uncalibrated energy simulation of typology C provided indicative evidence consistent with the expected thermal behavior of a high-thermal-mass bahareque envelope, with simulated minimum temperatures in the sleeping area within the American Society of Heating, Refrigerating and Air-Conditioning Engineers (ASHRAE) 55-2013 comfort range (T-min 18.80 °C). Collectively, these findings contribute quantified bioclimatic documentation of vernacular bahareque architecture in Ilincho, identifying attributes—encompassing solar control, spatial compactness, high-thermal-mass envelope performance, and use of locally sourced low-embodied-energy materials—that may inform sustainable rural housing discussions in the Ecuadorian Andes and comparable high-altitude mountain contexts. Its documentation in the indexed scientific literature constitutes a step toward recognizing this constructive heritage as a practical resource for low-carbon building policy. Full article

In today’s dynamic business environment, organizations are increasingly investing in strategies to protect themselves against information system violations. While these technologies offer remarkable benefits—boosting efficiency, productivity, and overall performance—they also bring significant risks, particularly regarding information security breaches. This study delves into the critical connections between technostress, techno-strain, and the violation of information security policies. Our research aims to shed light on how technostress, which is commonly experienced by engineers working in technology-intensive environments within the IT sector, drives information security violations. Importantly, we will also explore how techno-strain mediates this relationship. By focusing on engineers who are consistently engaged with advanced technology, we seek to answer essential questions about their experiences. It is worth noting that the requirements for information security technology can widely vary based on factors such as industry type, organizational structure, departmental roles, and cultural norms. Therefore, this study examines how technostress increases the likelihood of information security policy violations and how techno-strain mediates this relationship. Looking ahead, future research should consider both the broader institutional contexts and the individual characteristics that may shape the relationship between information security violations and technostress. Furthermore, understanding the repercussions of information security violations—stemming from technostress—on a company’s financial health is vital for organizations aiming to safeguard their assets and maintain a competitive edge. Emphasizing these insights can lead to more effective strategies for managing both technology and talent in the workplace. Full article

The degradation of works of art constitutes a significant problem for the preservation of cultural heritage. In the case of paintings, the observed alterations can be physical, chemical, or visual, affecting both the integrity and appearance of the artworks. Degradation compromises the authenticity, aesthetic legibility, and historical value of paintings, making the early monitoring of such issues, as well as the development of appropriate conservation and restoration strategies, essential. For an effective approach, the characterisation of the materials and techniques used by the artist, as well as the degradation processes inherent in the materials used, proves to be crucial. In this context, the application of artificial intelligence (AI) emerges as a non-invasive solution capable of detecting and predicting degradation in works of art. This bibliographic review aims to explore existing studies in this field in depth, with special attention to contemporary paintings considered as case studies. The methodology involved a systematic review of peer-reviewed studies, theses, and interdisciplinary databases, using keywords related to the topic under investigation (e.g., “degradation detection,” “artificial intelligence,” “craquelure segmentation”). The results indicate that artificial intelligence enables the early detection of degradations that may not yet be visible to the naked eye while also improving objectivity and consistency in the analysis of complex and irregular patterns typical of paintings. It became evident that there is a significant gap in the literature, regarding studies addressing the potential of AI for degradation detection specific to contemporary paintings. However, these could be a valuable case study given their potential material and technical heterogeneity, as well as their differences from traditional easel paintings. Full article

Enormous land exploitation is triggering strong urban growth, and this phenomenon is exacerbating the already existing problem of rising land surface temperatures. This leads to increased human activities and a disruption of the balance of natural ecosystems. The application of thermal remote sensing techniques is, in this context, helpful in learning about the condition of the earth’s surface and monitoring how it changes over time. This paper utilizes thermal data from 2000, 2010 and 2020, with supplementary data from 2024, to assess current trends in two different seasonal conditions (rainy period and low rainy period). Two different areas (urban and rural) of the central Vietnamese Province of Thua Thien-Hue have been analyzed to compare them. Processing Landsat-5 TM, Landsat-7 ETM+, Landsat-8 OLI/TIRS, and Sentinel-2 satellite images, a heat map of the study area was defined, considering hot spots and cold spots. As support for this analysis, spectral indexes have been developed for a better comprehension of the land cover change over the years and to provide a validation of the thermal analysis. This paper aims to assess the threat posed by the intensification of the urban heat island effect on cultural heritage sites. The case studies are represented by areas where there are urban growing and cultural heritage sites to be preserved, such as the UNESCO-listed Hue Citadel. Full article

This study proposes a theological-orthodox reinterpretation of contemporary management concepts—particularly “value innovation,” the “blue ocean strategy,” and the E.R.S.C. matrix—within the framework of church administration. Starting from the premise that such concepts cannot be directly imported into the ecclesial context without distortion, the paper argues for their “theological translation,” whereby their underlying logic is reoriented toward the service of the person, communion, and oikonomia. The analysis demonstrates that church administration cannot be understood as a neutral technical system, but as a form of diakonia, intrinsically linked to the ecclesial nature of the Church as the Body of Christ. Consequently, “value” is redefined not in utilitarian or economic terms, but as concrete good: the protection of human dignity, the strengthening of communion, the accessibility of liturgical and pastoral life, and the responsible use of resources. Within this framework, innovation is understood as a Christ-centered renewal of administrative practices, while differentiation (“blue ocean”) becomes a form of service rather than competition. The E.R.S.C. matrix is reinterpreted as an ascetical discipline of discernment, guiding administrative decisions through criteria rooted in theological anthropology and ecclesial ethics. Furthermore, the study addresses the ethical meaning of surplus, the role of transparency, the integration of virtue and competence in organizational culture, and the transformation of communication from image management into truthful witness. Ultimately, the paper argues that authentic church administration is not defined by procedural efficiency alone, but by its capacity to manifest, through structures and decisions, the love of Christ in concrete institutional life. Full article

This article develops a conceptual interdisciplinary model for research evaluation in the Social Sciences based on three core categories: Subject, Society, and Culture. It argues that conventional evaluation systems rely too heavily on quantitative metrics and, as a result, fail to capture the contextual, social, and epistemic complexity of knowledge production in this field. Drawing on an interdisciplinary analysis informed by complex thought and postcolonial theory, the article proposes a framework in which Subject refers to situated reflexivity and the role of relevant actors, Society emphasizes social relevance and public embeddedness, and Culture highlights epistemic plurality, local knowledge, and contextual legitimacy. The model is represented as a dynamic spiral, which underscores the revisable and context-sensitive character of evaluation. As a theoretical-conceptual contribution, the framework offers an alternative basis for broadening research assessment in the Social Sciences beyond productivity-driven and citation-based approaches. Full article

This article advances a transdisciplinary framework for planetary health education grounded in multispecies responsibility and Indigenous relational ontologies. Addressing the limitations of anthropocentric environmental paradigms, the paper proposes an expanded Stratified Relational Responsibility Model integrating ethical, ecological, and neurobiological dimensions of human–more-than-human relations. The framework bridges insights from environmental ethics, anthropology, and affective neuroscience to examine how relational awareness, emotional regulation, and embodied cognition shape pro-environmental behavior. Four pedagogical pillars are introduced to support behavioral transformation, emphasizing relational perception, affective attunement, ethical reflexivity, and collective responsibility. The article further discusses implementation challenges within Western educational contexts and highlights the need for culturally responsive adaptation. By situating human agency within multispecies networks, the model contributes to ongoing debates in planetary health and sustainability education, offering a theoretically robust and practically oriented approach to fostering ecological responsibility. Full article