Search Results (79,391)

This paper examines how artificial intelligence can support sustainability assessment in cultural industries, using national film industries as a test case. The Film Industry Sustainability Index (FISI) is introduced as a composite indicator covering cultural diversity, economic resilience, and Sustainable Development Goal (SDG) alignment for 42 national economies from 2005 to 2023. Knowledge-Enhanced Mamba (KE-Mamba), a selective state-space forecasting model, is then proposed to combine annual panel indicators with country-level film-industry knowledge graph (KG) embeddings and large language model (LLM)-derived screenplay-oriented narrative proxies from film synopses. To reduce factual errors in title-level narrative scoring, the LLM is anchored to verified United Nations Educational, Scientific and Cultural Organization (UNESCO) records and the European Audiovisual Observatory’s LUMIERE film-admissions database using rank-one model editing (ROME). On the 2020–2023 held-out test period, KE-Mamba achieves a composite FISI mean absolute error (MAE) of 0.0389, a mean absolute percentage error (MAPE) of 5.61%, and an $R^2$ of 0.934, outperforming autoregressive integrated moving average (ARIMA), tree-based, long short-term memory (LSTM), and base Mamba baselines. Additional robustness checks using a pre-pandemic split, two-way fixed-effects panel regression, alternative FISI weighting schemes, KG embedding ablations, and human validation of LLM narrative scores support the reliability of the proposed framework. Policy simulations are interpreted as model-based projected associations rather than causal estimates. The results show that knowledge-enhanced sequence models can provide transparent forecasting support for sustainable cultural-industry policy. Full article

Background: Open defecation (OD) has remained a threat to the attainment of SDG 6 (sanitation and hygiene). This study measured the level of exposure to the “Clean Nigeria, Use the Toilet” campaign against open defecation, determined the level of public knowledge about open defecation-related harms and diseases, ascertained the public attitude towards open defecation, and established the prevailing defecation practices and the perceived barriers to toilet usage in Ebonyi state, the most prevalent OD state in Nigeria. Methods: The study employed a survey design, using a structured questionnaire for data collection. The multi-stage sampling technique was employed in selecting the respondents from two randomly selected Local Government Areas (LGAs) in the state. Analysis was conducted using 384 valid responses. Results: The results were presented in simple percentage frequency tables and interpreted through the descriptive method, while the Chi-Square test was used to analyse the formulated hypotheses, using the decision rule of p < 0.05. The findings show a high level of awareness of the campaign against open defecation, through the radio and community engagements by environmental activists/NGOs, even though regular access to such information was limited. The results also showed inadequate knowledge of the public health implications of open defecation, whereas good knowledge of environmental consequences was reported. The study found favourable attitudes toward OD practice and persistent open defecation, and major barriers to toilet usage include the high cost of toilet construction, lack of access to toilet facilities, poor sanitation and management of available toilets, and perceived risks of contracting infection from public toilets. However, the Chi-Square values showed that the SBCC campaign was significantly associated with knowledge, attitude, and practice (p < 0.05). Conclusions: The study concluded that localised, culturally relevant and socio-demographically targeted communication interventions, grassroot advocacy, community watch, and neighbourhood taskforce on open defecation, in addition to the provision of aids for the construction of modern toilets with water facilities, are required to combat open defecation in Ebonyi and related contexts in Nigeria. Full article

Wear on historic staircases is often used as evidence for conservation assessment and historical interpretation, yet existing studies are largely descriptive and rarely provide a quantitative explanation of how observed wear relates to long-term pedestrian use. To address this limitation, this paper proposes a physics-constrained inversion framework for analyzing directional preference and wear-related usage regimes from geometric wear profiles of heritage staircases. An Archard-type wear model is extended to account for spatial footfall distribution, cumulative abrasion, material deterioration, and environmental loss, and the reconstruction problem is formulated as an inverse parameter estimation task. Bayesian uncertainty quantification is introduced to estimate posterior distributions, credible intervals, and parameter coupling. A unified workflow is developed for staircase geometry representation, reference surface reconstruction, profile extraction, regularized height field construction, forward simulation, and inverse solution. Nine synthetic scenarios with different usage levels and directional preferences are tested under 1%, 3%, and 5% noise, and the method is further applied to a publicly available three-dimensional heritage staircase model. Under 3% noise, profile correlation coefficients for three representative scenarios reach 0.9646, 0.9807, and 0.9868, indicating strong recoverability of geometric wear morphology under model-consistent conditions. The results indicate that directional preference, material hardness, and some degradation-related parameters are identifiable, whereas pedestrian volume and the wear coefficient show strong compensation. Overall, the proposed framework provides a quantitative basis for identifying directional asymmetry, analyzing parameter identifiability, and supporting geometry-based interpretation in heritage staircase studies. Full article

Osseointegration is defined as the structural and functional integration between alveolar bone and a dental implant. Photo-functionalization (PF) refers to ultraviolet (UV)-induced surface modifications of titanium implants, including changes in physicochemical properties and biological responsiveness. The aim of this study was to evaluate the effects of PF on early osteoblast responses related to osseointegration on titanium dental implants in vitro. We hypothesized that PF applied to titanium implants enhances early osteoblast responses related to osseointegration. Sixteen titanium dental implants were divided into two equal groups of eight: untreated (PF−) and PF-treated (PF+). PF+ implants were exposed to UV light at 172 nm for 10 s. An additional cell-only control group was incubated without an implant. All groups were cultured in vitro with SAOS-2 human osteoblast-like cells. Cell proliferation and viability were assessed using standard in vitro assays, and DNA damage was evaluated using the Terminal deoxynucleotidyl transferase [TdT] dUTP Nick End Labeling (TUNEL) assay. Early cellular responses related to osseointegration were assessed by evaluating adhesion-related vinculin levels and alkaline phosphatase (ALP) activity. After 24 h of incubation, cell proliferation was comparable between the groups, whereas after 48 h, cell number was significantly lower in the PF− group compared with the PF+ group and the control group (p = 0.039). Osteoblast viability was significantly lower in the PF− group than in the control group (53% vs. 94%, p = 0.002), while the PF+ group showed a numerically higher viability value than the PF− group (70% vs. 53%). TUNEL assay showed no statistically significant difference in DNA damage among the groups, although the PF− group showed a slightly higher TUNEL-positive cell ratio (p = 0.563). Vinculin levels were significantly higher in the PF+ group at both 24 and 48 h compared with the PF− group and control group (p < 0.0001). ALP activity increased significantly over time in cells incubated with PF-treated implants (p < 0.0001). Within the limitations of this exploratory pilot in vitro study, UV photo-functionalization was associated with more favorable early osteoblast-like cell responses on titanium dental implants, particularly in terms of proliferation, adhesion-related vinculin levels, and ALP response. PF did not increase TUNEL-positive cell ratios compared with untreated implants under the present experimental conditions. These findings should be interpreted as preliminary biological evidence and require confirmation through larger experimental designs, detailed physicochemical surface characterization, and in vivo validation. Full article

Listeria monocytogenes is a persistent foodborne pathogen capable of forming biofilms and surviving in food-processing environments. This study investigated the impact of high-pressure processing (HPP) at 200 and 400 MPa/5 min on biofilm viability, biomass, and expression of nine virulence-associated genes in L. monocytogenes strains (n = 6) belonging to the serogroups IIa (LM8, LM40, LM41) and IVb (LM14, LM47, LM48). The pressure levels applied were selected to represent sublethal HPP conditions (below 600 MPa) that allowed the survival of the strains and thus enabled the investigation of adaptive responses in cells that escape complete inactivation. Biofilms were cultivated on stainless-steel 304, polyethylene terephthalate, and polypropylene coupons under static conditions at 25 °C for 72 h and 168 h. Biofilm viability [log₁₀(CFU/cm²)] was assessed by plate count method and biomass quantified via the biofilm production index (BPI). The cultures were subjected to HPP treatment and their ability to form biofilms was re-evaluated. HPP significantly (p < 0.05) reduced biofilm viability and biomass on all types of surfaces tested. Gene expression analysis revealed a pressure-dependent (p < 0.05) modulation of flaA and sigB, while other virulence genes (agrA, agrC, actA, prfA, hly, inlB, and degU) were generally downregulated (gene expression ratio values below 1). Serogroup IVb strains exhibited enhanced stress responses and lower biofilm survival on polyethylene terephthalate and polypropylene surfaces. These findings demonstrate that HPP modulates both phenotypic and genotypic traits linked to L. monocytogenes persistence, emphasizing the need to optimize pressure parameters and surface materials to prevent biofilm formation in HPP-treated food systems. Full article

The fast-growing cyanobacterium Synechococcus sp. PCC 11901 is emerging as a promising chassis for photosynthetic biomanufacturing. Here we report recombinant protein production in PCC 11901 via signal peptide-mediated secretion, enabling direct recovery of target proteins from the culture medium without cell disruption. Seven signal peptides spanning both Sec and Tat pathways are screened using eYFP as a reporter, with secretion quantified daily over seven days by fluorescence measurements. FutA, belonging to the Tat pathway from Synechocystis sp. PCC 6803, achieves 92.2% extracellular export by day 7, substantially outperforming all Sec candidates, including the best Sec signal peptide thermitase from Cyanobacterium aponinum PCC 10605 (55.7%). Signal peptide-bearing strains exhibit growth reductions of up to 26% relative to the wild-type, with FutA most affected, indicating a general metabolic cost correlated with secretion efficiency. The best-performing signal peptides from both pathways, FutA and thermitase, are validated with secretion of lichenase. Notably, the rank order of signal peptide performance is reversed for lichenase: thermitase demonstrates 2.6-fold higher extracellular activity than FutA, indicating that optimal signal peptide selection is cargo-dependent. These results establish PCC 11901 as a secretion-competent chassis and provide a rational framework for matching signal peptide pathways to target protein properties. Full article

The housing sector in major cities is facing escalating challenges due to rapid population growth and land scarcity. Consequently, vertical growth has been adopted as a strategic solution to optimize land use while balancing economic, social, and environmental needs. This study examines the phenomenon of vertical growth of the Dammam Metropolitan Area (DMA) in Saudi Arabia, from an urban sustainability perspective, focusing on evaluating the current state of multi-story buildings, their determinants, and their impact on quality of life and infrastructure efficiency. This study utilizes a systematic review methodology and a conceptual approach to develop an integrated framework for sustainable vertical growth. Furthermore, an empirical validation was conducted by projecting this framework onto vertical housing projects in Dammam, focusing on challenges related to design, construction quality, shared service management, and the suitability of apartments for family needs. The results indicate that the shift toward vertical growth achieves land-use efficiency, limits random horizontal expansion, and provides economic opportunities. However, it faces social and cultural constraints, most notably the resistance of some families to changing traditional ownership patterns, limited privacy and green spaces, and challenges in building maintenance and operations. The study highlights the importance of integrating urban planning, governance, architectural design, and infrastructure to ensure the sustainability of vertical growth and provide suitable housing alternatives. The study recommends further field research to assess social acceptance, improve quality-of-life indicators, and develop policies encouraging sustainable vertical expansion in alignment with Saudi Vision 2030 and the 2030 Sustainable Development Goals (SDGs), ensuring cities are more resilient, efficient, sustainable, and liveable. Full article

The application of continuous carbon fiber (CCF) can reinforce the mechanical properties of 3D-printed parts, but the effect of reinforcement layer distribution on composite performance remains unclear. This study investigates the effect of concentrated and separated distributions of CCF layers with different numbers of reinforcement layers. Tensile and flexural tests are conducted in accordance with ASTM D5083 and ASTM D790, respectively. Under the conditions of a solid-filled matrix (Onyx) and 0° CCF deposition, both concentrated and separated CCF layers improve several mechanical properties. Compared with pure Onyx, one-layer CCF increases the tensile modulus by about six times and more than doubles the tensile strength. Increasing the CCF volume leads to further increases in these properties. With concentrated three-layer CCF, the tensile modulus and tensile strength reach 7.153 ± 0.090 GPa and 109.045 ± 5.124 MPa, respectively. For flexural properties, separated two- and three-layer CCFs significantly improve the tangent modulus of elasticity from 0.467 ± 0.106 GPa for pure Onyx to 2.246 ± 0.333 GPa and 3.394 ± 0.081 GPa, respectively. This study also compares the tensile and flexural strength-to-weight ratio of all specimen groups and analyzes the failure mechanisms based on macroscopic fracture appearance. The results can provide guidance for selecting appropriate CCF layer distribution strategies to reinforce composites in different applications. Full article

Scaffolds designed for mechanically demanding soft tissue engineering applications should integrate mechanical support, efficient mass transfer, and good cellular compatibility. This work presents a one-pot method based on “radical-free click chemistry + carbodiimide coupling” to produce a double-network (DN) PEG–alginate cryogel. The PEG network is formed by a Michael addition reaction between thiol-based crosslinker and 8-arm PEG-acrylate. The second network is covalently crosslinked through EDC/NHS-mediated coupling of carboxyl groups in alginate and adipic acid dihydrazide (AAD). The subsequent freezing and gelation of the gel precursor at sub-zero temperatures results in an ice templated cryogel with an interconnected macroporous network. These cryogels demonstrate high elasticity, compressive modulus and rapid swelling equilibrium in aqueous environments, as well as controlled degradation under physiological conditions. Compared to the classical Ca²⁺ ion crosslinking systems, the covalent linking of the alginate in the double-network cryogel shows advantages in mechanical and structural stability. In addition, it is cell-compatible and allows culture of mesenchymal stem cells (MSCs) with homogeneous infiltration. Furthermore, the double-network cryogels supports chondrogenic differentiation of MSCs upon treatment with chondrogenic media or macrophage-conditioned media for a short period of time. These results indicate that crosslinking chemistry and polymer composition can be used to modulate the balance between mechanical performance and degradation behavior, while maintaining cytocompatibility and an interconnected macroporous network, thereby providing a scaffold design strategy for applications that require coordinated mechanical support and mass transfer, such as cartilage-related tissue engineering. Full article

In this case study, we examined a school in Northern Norway that has integrated outdoor learning as a core element of its pedagogical practices. To develop a comprehensive understanding of the role of outdoor learning and the factors contributing to the school’s success, we conducted semi-structured interviews with stakeholders of outdoor learning: six students, three teachers, one teaching assistant, and the principal. Our interviews were thematically analyzed using a whole-school approach framework, and our findings indicate that outdoor learning is embedded in the school’s identity. The regularity of outdoor learning for all students, with support from the school’s leadership and committed teachers, ensures predictability and continuity. Students and staff are broadly positive about outdoor learning and report that it strengthens student–teacher relationships. Outdoor learning is understood as interdisciplinary, and the practice enhances both academic learning and environmental awareness. Nevertheless, we identified limited opportunities and a weak culture for sharing outdoor learning practices among teachers. The school therefore aims to develop a progression plan for outdoor learning and to facilitate greater sharing to strengthen the professional community and improve coherence. This case study contributes to the literature by specifying organizational and contextual conditions for successful implementation and by highlighting the need to align outdoor and indoor teaching. Sustained outdoor learning requires holistic support from everyone involved in the school community. Full article

Introduction: As global cancer incidence and survival rates continue to rise, understanding the experiences and needs of individuals in the survivorship phase is critical to inform policies that promote equitable care and adequate support for cancer survivors. Objective: The objective of this study was to examine the lived social and workplace experiences of cancer survivors in Newfoundland and Labrador (Canada) using a qualitative research design. Methods: The study was open to cancer survivors of majority age who resided in Newfoundland and Labrador after their diagnosis. Between June 2023 and August 2024, twenty-five individuals participated in the study. Data were collected virtually through focus groups, individual interviews, or written responses. Demographic and socioeconomic characteristics of participants were collected via a survey. Thematic analysis was performed on all qualitative data. Two patient partner investigators informed the research throughout the entire project. Results: Data were rich and diverse, revealing a range of positive and negative experiences in social and workplace settings. Major themes included stigma in social and workplace environments, financial toxicity, workplace accommodations, social support and information needs. Young participants had unique challenges. Participants offered recommendations aimed at enhancing available supports and improving the quality of life of cancer survivors. Overall, findings highlight shared experiences across different regions and cultures while also painting the local context. Discussion: The results of this study reveal diverse experiences among cancer survivors within social and workplace settings. The findings and resulting recommendations can inform meaningful improvement to policies and programs, thus promoting equity and enhancing the lived experiences of cancer survivors. Full article

The Maule Region in Chile possesses a rich cultural heritage associated with petroglyphs created by ancient hunter-gatherer inhabitants. This rock art has suffered damage over time due to natural and anthropic causes. Fostering positive attitudes toward petroglyphs may influence behavioral intentions related to their preservation. This study evaluates an augmented reality card game developed to promote positive attitudes toward the rock art heritage of the Maule Region, examining its usability and the effects of incorporating augmented reality elements. The game achieved a System Usability Scale (SUS) score of 79.7 (SD = 14.2), corresponding to an A-grade on the Sauro-Lewis curved grading scale, indicating good usability.Participants in the game condition showed higher heritage attitudes than controls (M = 6.13, SD = 0.80, $t_{\left(24\right)}$ = −2.33, p = 0.028). Augmented reality enhanced attitudes at moderate levels of usability (B = −1.02, p = 0.043), but produced no detectable main effect in mean comparisons alone. The results indicate that the game constitutes a system with adequate usability, effective in fostering positive attitudes toward cultural heritage, and that augmented reality enhances attitudinal outcomes under conditions of moderate perceived usability. Full article

Digital twins have become a central paradigm for cultural heritage documentation, monitoring, and preventive preservation. Yet, when cultural heritage systems promise prediction, simulation, intervention planning, and decision support, a more explicit account is needed of the computational commitments behind such claims. This position paper proposes the notion of the heritage world model as a conceptual and architectural abstraction that uses the semantic digital twin as its representational layer and extends it toward prediction, memory, uncertainty-aware reasoning, and intervention evaluation. We define a heritage world model as a structured, temporally updated, semantically grounded, and action-aware model of a heritage asset and its preservation environment, capable of integrating observations, estimating latent risk states, predicting plausible future trajectories, and evaluating interventions under uncertainty. The paper does not present a validated deployed system. Rather, it clarifies the architectural conditions under which a decision-support digital twin infrastructure could support the kind of world-model-like preservation system proposed here. It further argues that such a model becomes operationally meaningful only when it includes a human-supervised controller layer that maps semantic state, predicted risk trajectories, uncertainty, memory, and institutional constraints into preservation-relevant actions, alerts, monitoring adaptations, or requests for expert review. Sensor data, remote sensing, computational models, risk assessments, policies, and conservation actions are interpreted as possible observational, dynamic, and intervention layers of a heritage world model. The paper reviews adjacent work in heritage digital twins, semantic and reactive ontologies, risk-aware preservation, agentic AI, and modern AI world models, and proposes a research agenda for moving toward predictive, memory-bearing, and intervention-aware preservation intelligence. Full article

Analysis of freely moving Drosophila captures complex movement behaviors. However, previous experiments have been limited by the inability to distinguish between falls and downward jumps (downjumps). Here, individual flies moving freely in a culture vial were recorded using a single 58 fps video camera. Upward jumps were readily identified by positive movement in the vertical direction. Several statistical and machine learning methods were used to distinguish between falls and downjumps, including Principal Component Analysis (PCA), K-Means Clustering, Uniform Manifold Approximation and Projection (UMAP), Hierarchical Density-Based Spatial Clustering with Applications to Noise (HDBSCAN) and Shapley Additive Explanations (SHAP). Falls were abundant and characterized by an initial velocity consistent with simple acceleration due to gravity. Downjumps were more rare, and were characterized by a greater initial velocity, indicating active propulsion by the fly. Aged flies took longer to resume movement after a fall, suggesting possible negative effects of falls. Falls in young w[1118]-strain flies exhibited mid-event velocities that were lower than expected, indicating some compensatory behavior that was reduced in aged flies. These methods should facilitate future studies of the effects of aging and neurodegenerative disease models on locomotor behaviors and falls, including the testing of potential interventions. Full article

Oyster health is important for aquaculture productivity and sustainability. In Thailand, the white scar oyster, Crassostrea belcheri, is being promoted for cultivation, yet its health status has not been compared between research breeding and community farming sites. This study evaluated histopathological features, ultrastructure, apoptosis, and defender against apoptotic death 1 (dad1) gene expression in sexually mature C. belcheri collected from these two sites. Gill tissues were examined by histology, transmission electron microscopy, TUNEL assay, and gene expression analysis, while organ condition was assessed using a Health Assessment Index (HAI). The proportion of TUNEL-positive cells in the gills and mantle differed significantly between sites (p < 0.05), with higher levels in oysters from the farming site. In contrast, TUNEL-positive cells in the digestive gland did not differ significantly between sites, although brown cells were observed only in the digestive gland of oysters from the breeding site, suggesting possible physiological stress. To assess the expression level of dad1 in oysters cultured under different conditions, RT-qPCR revealed no significant difference between the two sites. The breeding site also had lower temperature and salinity than the farming site. Overall, these findings suggest that site-specific environmental conditions may influence gill health and stress-related responses in C. belcheri, providing baseline information for oyster health assessment and aquaculture management. Full article