Next Article in Journal
The In Vivo Existence Forms of Engeletin and Their Anti-Hyperuricemia Activity
Previous Article in Journal
Wolffia globosa Ethanolic Extract Protects Against Bisphenol A-Induced Osteoblast Dysfunction via Antioxidant Defense, Apoptosis Inhibition, and β-Catenin Modulation
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 27
Issue 12
10.3390/ijms27125351
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Comparative Health Assessment of Crassostrea belcheri from Breeding and Farming Sites in Thailand: Histopathological, Apoptotic, and Molecular Evidence

by
Supatcha Chooseangjaew
1,
Suwat Tanyaros
2,
Narit Thaochan
3,
Sirilak Dusitsittipon
4,
Natthawut Charoenphon
5,
Gen Kaneko
6,
Supapong Imsonpang
7,
Nabhasbhichayabha Daewang
8,
Kitipong Angsujinda
9,
Kitiya Kongthong
10 and
Sinlapachai Senarat
11,12,*
1
Aquaculture Technology Program, Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang 92150, Thailand
2
Marine Science Program, Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang 92150, Thailand
3
Agricultural Innovation and Management Division, Faculty of Natural Resource, Prince of Songkla University, Songkhla 90110, Thailand
4
Department of Parasitology and Entomology, Faculty of Public Health, Mahidol University, Bangkok 10400, Thailand
5
Department of Anatomy, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand
6
College of Natural and Applied Science, University of Houston-Victoria, Victoria, TX 77901, USA
7
Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand
8
Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
9
Aquatic Resources Research Institute, Chulalongkorn University, Bangkok 10330, Thailand
10
Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand
11
Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand
12
Center for Integrative Animal Tissue Biology and Innovation (iTissue-PSU), Prince of Songkla University, Songkhla 90110, Thailand
 
add Show full affiliation list
 
remove Hide full affiliation list
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2026, 27(12), 5351; https://doi.org/10.3390/ijms27125351 (registering DOI)
Submission received: 12 May 2026 / Revised: 7 June 2026 / Accepted: 8 June 2026 / Published: 13 June 2026
(This article belongs to the Special Issue Molecular Research on Aquatic Organisms)
Versions Notes

Abstract

Oyster health is important for aquaculture productivity and sustainability. In Thailand, the white scar oyster, Crassostrea belcheri, is being promoted for cultivation, yet its health status has not been compared between research breeding and community farming sites. This study evaluated histopathological features, ultrastructure, apoptosis, and defender against apoptotic death 1 (dad1) gene expression in sexually mature C. belcheri collected from these two sites. Gill tissues were examined by histology, transmission electron microscopy, TUNEL assay, and gene expression analysis, while organ condition was assessed using a Health Assessment Index (HAI). The proportion of TUNEL-positive cells in the gills and mantle differed significantly between sites (p < 0.05), with higher levels in oysters from the farming site. In contrast, TUNEL-positive cells in the digestive gland did not differ significantly between sites, although brown cells were observed only in the digestive gland of oysters from the breeding site, suggesting possible physiological stress. To assess the expression level of dad1 in oysters cultured under different conditions, RT-qPCR revealed no significant difference between the two sites. The breeding site also had lower temperature and salinity than the farming site. Overall, these findings suggest that site-specific environmental conditions may influence gill health and stress-related responses in C. belcheri, providing baseline information for oyster health assessment and aquaculture management.
Keywords: aquaculture; environmental stress; Crassostrea belcheri; white scar oyster; histopathology; apoptosis; TUNEL assay aquaculture; environmental stress; Crassostrea belcheri; white scar oyster; histopathology; apoptosis; TUNEL assay

Share and Cite

MDPI and ACS Style

Chooseangjaew, S.; Tanyaros, S.; Thaochan, N.; Dusitsittipon, S.; Charoenphon, N.; Kaneko, G.; Imsonpang, S.; Daewang, N.; Angsujinda, K.; Kongthong, K.; et al. Comparative Health Assessment of Crassostrea belcheri from Breeding and Farming Sites in Thailand: Histopathological, Apoptotic, and Molecular Evidence. Int. J. Mol. Sci. 2026, 27, 5351. https://doi.org/10.3390/ijms27125351

AMA Style

Chooseangjaew S, Tanyaros S, Thaochan N, Dusitsittipon S, Charoenphon N, Kaneko G, Imsonpang S, Daewang N, Angsujinda K, Kongthong K, et al. Comparative Health Assessment of Crassostrea belcheri from Breeding and Farming Sites in Thailand: Histopathological, Apoptotic, and Molecular Evidence. International Journal of Molecular Sciences. 2026; 27(12):5351. https://doi.org/10.3390/ijms27125351

Chicago/Turabian Style

Chooseangjaew, Supatcha, Suwat Tanyaros, Narit Thaochan, Sirilak Dusitsittipon, Natthawut Charoenphon, Gen Kaneko, Supapong Imsonpang, Nabhasbhichayabha Daewang, Kitipong Angsujinda, Kitiya Kongthong, and et al. 2026. "Comparative Health Assessment of Crassostrea belcheri from Breeding and Farming Sites in Thailand: Histopathological, Apoptotic, and Molecular Evidence" International Journal of Molecular Sciences 27, no. 12: 5351. https://doi.org/10.3390/ijms27125351

APA Style

Chooseangjaew, S., Tanyaros, S., Thaochan, N., Dusitsittipon, S., Charoenphon, N., Kaneko, G., Imsonpang, S., Daewang, N., Angsujinda, K., Kongthong, K., & Senarat, S. (2026). Comparative Health Assessment of Crassostrea belcheri from Breeding and Farming Sites in Thailand: Histopathological, Apoptotic, and Molecular Evidence. International Journal of Molecular Sciences, 27(12), 5351. https://doi.org/10.3390/ijms27125351

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Article metric data becomes available approximately 24 hours after publication online.
Back to TopTop