Search Results (61)

The Middle-Wave Infrared Imaging Spectrometer for Target Asteroids (MIST-A) will be launched in 2028 aboard the Emirates Mission to the Asteroid belt (EMA) and will operate in the 2–5 μm spectral range to study the asteroids’ surface composition and thermo-physical properties. MIST-A’s Optical Head (OH) design is inherited from the Jovian IR Auroral Mapper (JIRAM), from which the instrument also received two spare Hybrid-Thinned Mercury-Cadmium-Telluride (MCT) photodetectors: the Engineering Model EM2 and the Flight Spare FS1. These are tested to assess their performance after a long period of storage. The laboratory setup for testing both detectors consists of a blackbody and a cryostat which houses the focal plane, maintained at temperatures of 85 K, its nominal operative temperature, and 90 K. Two sets of measurements are performed: (1) characterization of the dark current at different integration times (0 ms, 224 ms, 448 ms, 672 ms, 869 ms, 1120 ms); (2) verification of the detectors’ response linearity, measuring a blackbody at different temperatures (from 50 °C to 100 °C), including ambient temperature (25 °C, with the blackbody turned off). The results of these tests confirm that both models are fully operational and allow us to evaluate the consequences of the years of inactivity on their performance. Through a detailed analysis of the detectors’ properties and a comparison study with the results of the sensors’ first characterization performed by their producer in 2009, we come to the conclusion that both instruments are able to fulfill MIST-A’s scientific requirements. The FS1 displays a better performance with respect to the EM2 and for this has been selected as MIST-A’s Flight Model. Full article

Background: Efficient tissue preservation methods are critical for accurate and quantitative microscopical examination in histopathology. Quantitative image analysis and cell counts are essential to translational research with direct implications for therapeutic decision-making. This study aims to compare the histomorphological quality of formalin-fixed paraffin-embedded (FFPE) and fresh frozen sections (FFS) in terms of tissue recognizability, physical integrity, as well as cell counts of lymphocytes, granulocytes, giant cells, and blood vessels Methods: A total of 142 skin biopsies were analyzed, with 88 FFPE and 54 with FFS. The biopsies were stained with HE and ASD. The sections were evaluated for recognizability and physical appearance, and categorized as either clearly recognizable or indistinctly recognizable, and fully intact, folded, or torn. Suited high-power fields were identified to compare the number of different cell types between the two preservation techniques. Results: FFPE showed significantly higher morphological quality than FFS in maintaining both recognizability (88.64% vs. 44.44%, p < 0.001) and physical integrity, with more sections remaining fully intact (77.27% vs. 22.22%, p < 0.001). Additionally, paraffin sections showed higher counts of lymphocytes and blood vessels (both p < 0.001) with significant statistical differences. Conclusion: The findings suggest that FFPE provides superior tissue preservation compared to FFS, particularly in maintaining structural integrity and cellular detail. This study underlines the importance of choosing appropriate embedding techniques to optimize histological evaluations, especially in clinical settings where quantitative analyses are crucial. Full article

Background and Clinical Significance: The paper investigates the use of the Histolog^® Scanner, a confocal microscopy–based device, as a potential tool for intraoperative neuropathological diagnosis of brain tumors. Traditional intraoperative diagnosis, relying on frozen sections and squash preparations, can introduce artifacts and consume valuable tissue. The Histolog^® Scanner offers a plug-and-play solution capable of acquiring high-resolution images of fresh tissue surfaces in minutes while preserving tissue for further histological or molecular analyses. Cases Presentation: Three clinical cases—two women and one-man, mean age 57.3 years—undergoing neurosurgery for distinct brain lesions were included. Tissue samples were immersed in fluorescent dye, rinsed, and immediately analyzed with the Histolog^® Scanner before standard intraoperative histopathology. In the first case, a glioblastoma wild-type, traditional methods struggled to define tumor margins, whereas the device provided rapid, detailed imaging to guide resection. In the second case, a meningioma, the scanner confirmed lesion identity quickly, eliminating the need for a cryostat and reducing artifacts. In the third case, a brain metastasis, integration with cytological apposition allowed simultaneous assessment of lesion margins and nature without freezing the tissue. Conclusions: The Histolog^® Scanner demonstrated multiple advantages: rapid intraoperative use, clear margin visualization, preservation of tissue for subsequent analyses, reduce unnecessary resection, thereby helping to lower the risk of recurrence. This device may complement standard intraoperative methods, enhancing diagnostic accuracy and influencing postoperative treatment planning. Overall, the Histolog^® Scanner represents an innovative tool combining speed, precision, and tissue preservation, suggesting a promising role in establishing a new standard for intraoperative neurosurgical diagnosis. Full article

Two outstanding problems of particle physics and cosmology, namely the strong-CP problem and the nature of dark matter, can be solved with the discovery of a single new particle, the axion. The modular high magnetic field and flux hybrid magnet platform of LNCMI-Grenoble, which was recently put in operation up to 42 T, offers unique opportunities for axion/axion-like particle search using Sikivie-type haloscopes. In this paper, the focus will be on the 350–600 MHz frequency range corresponding to the 1–3 μeV axion mass range requiring a large-bore RF-cavity. It will be built by DMAG and integrated within the large-bore superconducting hybrid magnet outsert, providing a central magnetic field up to 9 T in 812 mm warm bore diameter. The progress achieved by Néel Institute in the design of the complex cryostat with its double dilution refrigerators to cooldown below 50 mK the ultra-light Cu RF-cavity of 650 mm inner diameter and the first stage of the RF measurement chain are presented. Perspectives for the targeted sensitivity, assuming less than 2-year integration time, are recalled. Full article

The Volumetric Neutron Source (VNS) is a pivotal facility proposed for advancing fusion nuclear technology, particularly for the qualification of breeding blanket systems, a key component of DEMO and future fusion reactors. This study focuses on the design and optimisation of the VNS Thermal Shield, adopting a multidisciplinary approach to address its thermal and structural behaviours. The Thermal Shield plays a crucial role in protecting superconducting magnets and other cryogenic components by limiting heat transfer from higher-temperature regions of the tokamak to the cryostat, which operates at temperatures between 4 K and 20 K. To ensure both thermal insulation and structural integrity, multiple design iterations were conducted. These iterations aimed to reduce electromagnetic (EM) forces induced during magnet charge and discharge cycles by introducing strategic cuts and reinforcements in the shield design. The optimisation process included the evaluation of various aluminium alloys and composite materials to achieve a balance between rigidity and weight while maintaining structural integrity under EM and mechanical loads. Additionally, an integrated thermal study was performed to ensure effective temperature management, maintaining the shield at an operational temperature of around 80 K. Cooling channels were incorporated to homogenise temperature distribution, improving thermal stability and reducing thermal gradients. This comprehensive approach demonstrates the viability of advanced material solutions and design strategies for thermal and structural optimisation. The findings reinforce the importance of the VNS as a dedicated platform for testing and validating critical fusion technologies under operationally relevant conditions. Full article

Microscale systems have been underexplored in contemporary regenerative therapies developed to treat vision loss. The pairing of in vitro cell systems with optical fluorescent imaging provides unique opportunities to examine the infiltration of donor stem cells needed for successful transplantation therapies. A parallel eye device was developed to provide electric field (EF) stimulation to guide the migration of cells within 3D eye facsimiles synthesized from different ocular biomaterials. Cell infiltration within facsimiles was rapidly resolved using confocal microscopy to eliminate dependence on the cryostat sectioning commonly used for cell study. Moreover, EF stimulated galvanotaxis of donor cells within different depths of eye facsimiles. Optical imaging provided rapid resolution of z-stack images at physiologically appropriate depths below 500 microns. This study demonstrates that paired microscale–optical systems can be developed to elucidate understudied transplantation processes and improve future outcomes in patients. Full article

This paper describes the development and validation of an apparatus for the realization of the triple point of argon (83.8058 K), with a novel automatic pressure control system for the liquid nitrogen cryostat. The automatic pressure controller, together with custom-made software, was developed and tested in the Laboratory for Process Measurement at the Faculty of Mechanical Engineering and Naval Architecture, University of Zagreb (FSB-LPM). Performance testing and characterization of the automatic pressure controller confirmed its suitability for precise and reliable control of gauge pressure in the cryostat. The characteristics and uncertainty of the measurement setup for the realization of the triple point of argon were validated through a bilateral hybrid comparison with the Laboratory of Metrology and Quality at the Faculty of Electrical Engineering, University of Ljubljana (MIRS/UL-FE/LMK). A long-stem quartz-sheathed standard platinum resistance thermometer was used as a transfer standard. The realizations of the International Temperature Scale (ITS-90) were compared in the subrange from the triple point of argon to the triple point of water. The comparison results show that resistance ratio (W) values determined by FSB-LPM at the fixed points of argon and mercury deviate from the MIRS/UL-FE/LMK values, within the determined combined uncertainty of the comparison. Full article

While the design of a cryostat is being developed, one of the most relevant sub-systems is the internal supporting system that sustains the cooled component. According to the literature, the arrangement and number of supports chosen often result in a multi-leg over-constrained architecture. These are usually studied by means of finite element analysis tools alone, which makes studies like the optimization of supporting systems computationally expensive. This paper proposes a more structured and general analytical model compared to the existing models for this application. The proposed lumped parameter model allows designers to study the influence of external loads, pre-load, and cool-down on stress levels and deformation status of the supports of the cryogenic device as well as the consequent misalignment of the cooled component. The general lumped parameter model for n tie-rods of different shapes, dimensions, and materials is proposed. Two particularized models of eight and eleven supports are validated by comparing the results with those from standard finite element analysis software. Results show that the proposed model has a strong agreement with finite element simulations, and the median of relative errors is about 1.4%. This accuracy is obtained for models of randomly arranged supports, which proves the effectiveness of the model in predicting results even for non-symmetrical support configurations. Comparable and accurate results are obtained, which are about 130 times faster than in finite element analysis, thus proving the effective reduction in computational cost. Additionally, the proposed code lets designers change input parameters in a quicker and reliable way. Full article

Background/Objectives: Catha edulis, commonly known as khat, is used for its psychoactive effects and is considered a natural amphetamine. The current study investigated the metabolomic profile in the cerebellum of mice after repeated exposure to khat and evaluated the effects of clavulanic acid on the metabolomic profile in the cerebellum in khat-treated mice. Methods: Male C67BL/6 mice that were 6–9 weeks old were recruited and divided into three groups: the control group was treated with 0.9% normal saline for 17 days; the khat group was given khat extract at a dose of 360 mg/kg via the intraperitoneal (i.p) route for 17 days; and another khat group was treated with khat for 17 days and clavulanic acid at a dose of 5 mg/kg for the last 7 days (days 11–17). At the end of the 17th day, the animals were sacrificed, and their brains were immediately collected and stored at −80 °C. The cerebellum region of the brain was isolated in each group by micropuncture using cryostat and underwent a metabolomics study via Gas Chromatography/Mass Spectroscopy (GC/MS). The total peak area ratios of the selected metabolites in the cerebellum after repeated exposure to the khat extract were significantly reduced (p < 0.05) and treatment of the khat group with clavulanic acid significantly increased (all p < 0.05) the total peak areas ratios of the selected metabolites when compared to their corresponding areas in the alternative khat group. These levels of selected metabolites were further confirmed by observing the metabolite peak area ratios and performing a heat map analysis and a principal compartment analysis of the samples in the cerebellum. Results: A network analysis of altered metabolites in the cerebellum showed a strong correlation between the different metabolites, which showed that an increase in one metabolite can modulate the levels of others. An analysis using the MetaboAnalyst software revealed the involvement of selected altered metabolites like lactic acid in many signaling pathways, like gluconeogenesis, while enrichment analysis data showed altered pathways for pyruvate metabolism and disease pathogenesis. Finally, a network analysis showed that selected metabolites were linked with other metabolites, indicating drug–drug interactions. Conclusions: The present study showed that repeated exposure of mice to khat altered the levels of various metabolites in the cerebellum which are involved in the pathogenesis of different diseases, signaling pathways, and interactions with the pharmacokinetic profile of other therapeutic drugs. The treatment of khat-treated mice with clavulanic acid positively modified the metabolomics profile in the cerebellum and increased the levels of the altered metabolites. Full article

The use of non-cryogenic certified commercial electronics for cryogenic applications may be attractive due to their cost and availability, but it also carries risks related to reliability, performance and thermal compatibility. The decision to use commercial components that are not certified for cryogenics instead of components specifically designed for such applications must be carefully weighed based on specific project needs and risk tolerances. This work presents the characterisation of an attenuator circuit at cryogenic temperatures used in a microwave kinetic inductance detector (MKID) readout system. In order to characterise the operation of the attenuator at cryogenic temperatures and because the circuit works at frequencies up to 40 GHz, a specific microwave PCB has been designed. The cooling system used consists of a cryostat, all the connectors, cables, a vacuum pump, a compressor, pressure and temperature sensors, a temperature control system and a cold head operating in a closed helium gas cycle according to the Gifford–McMahon principle. The circuit was tested and characterised at temperatures ranging from 296.5 K to 83.6 K. Full article

There are many applications of soybean lecithin (SL) and cholesterol-loaded cyclodextrin (CLC) in sperm freezing processes. To the best of our knowledge, there have been few cases of the combined use of SL and CLC in freezing rooster semen. We investigated the effects of CLC, SL, and their combination on rooster sperm cryodamage. Three experiments were conducted: experiment 1, SL (0.5%, 1%, 1.5%, 2.0%); experiment 2, CLC (1.25 mg, 2.5 mg, 5 mg, 10 mg, 15 mg); and experiment 3, CLC + SL (2.5 mg + 0.25%, 2.5 mg + 0.5%, 2.5 mg + 1%, 2.5 mg + 1.5%). Semen samples were cryopreserved using a programmed cryostat, followed by the determination of post-thaw sperm quality, antioxidant indices, and hatching. The results showed that the combination of 2.5 mg CLC + 0.5% SL had the most significant synergistic effect on cryodamage, and the viability (56.69%), motility (54.35%), mitochondrial activity (54.23%), plasma membrane integrity (53.52%), acrosome integrity (54.71%), and antioxidant activity (MDA concentration: 5.65 nmol/mL; SOD activity: 152.73 U/mL) were significantly greater than those of the other combinations (p < 0.05). Nevertheless, the combined CLC and SL addition group did not substantially increase the fertilization and hatching rates of frozen semen compared with the addition of 2.5 mg CLC. In conclusion, the addition of 2.5 mg CLC and 2.5 mg CLC + 0.5% SL enhanced the quality and fertility of frozen rooster sperm. Full article

The application of terahertz (THz) science in industrial technology and scientific research requires efficient THz detectors. Such detectors should be able to operate under various external conditions and conform to existing geometric constraints in the required application. Pyroelectric THz detectors are among the best candidates. This is due to their versatility, outstanding performance, ease of fabrication, and robustness. In this paper, we propose a compact pyroelectric detector based on a bioriented poled polyvinylidene difluoride film coated with sputtered metal electrodes for in situ absorption measurement at cryogenic temperature. The detector design was optimized for the registration system of the electron paramagnetic resonance (EPR) endstation of the Novosibirsk Free Electron Laser facility. Measurements of the detector response to pulsed THz radiation at different temperatures and electrode materials showed that the response varies with both the temperature and the type of electrode material used. The maximum signal level corresponds to the temperature range of 10–40 K, in which the pyroelectric coefficient of the PVDF film also has a maximum value. Among the three coatings studied, namely indium tin oxide (ITO), Au, and Cu/Ni, the latter has the highest increase in sensitivity at low temperature. The possibility of using the detectors for in situ absorption measurement was exemplified using two typical molecular spin systems, which exhibited a transparency of 20–30% at 76.9 cm⁻¹ and 5 K. Such measurements, carried out directly in the cryostat with the main recording system and sample fully configured, allow precise control of the THz radiation parameters at the EPR endstation. Full article

SU-8 is an emerging polymer material for integrated optical circuits that has demonstrated good structural properties in a cryogenic environment. In this article, we investigate the thermo-optical properties of SU-8 for a wavelength $\lambda =850\mathrm{n}\mathrm{m}$, from room temperature to cryogenic temperature down to 14 K. To measure the material properties, we designed and fabricated SU-8 racetrack resonators via electron beam lithography. While cooling the device in a closed-cycle cryostat, we measured the resonance spectrum as a function of the temperature from which we determined the temperature-induced variations of the group and effective indices of the waveguide. With the aid of waveguide eigenmode simulations, we used these data to derive the temperature dependence of the SU-8 refractive index $n_{SU-8}\left(T\right)$. At room temperature (T~295 K), the thermo-optic coefficient ${dn}_{SU-8}/dT=-5.3\pm {0.2\times {10}^{-5}\mathrm{K}}^{-1}$. At low temperature (T~14 K), ${dn}_{SU-8}/dT=-1.27\pm {0.05\times {10}^{-4}\mathrm{K}}^{-1}$. Our research shows the potential of SU-8 photonics in a cryogenic environment, suitable for the integration with quantum light sources emitting in the near infrared (NIR). Full article

A liquid helium-free cryostat for radio frequency (RF) test of the superconducting cavity is designed and constructed. Gifford-Mcmahon (G-M) cryocoolers are used to provide cooling capacity, and the heat leakage at 4 K is less than 0.02 W. Vertical and horizontal tests of two Nb₃Sn cavities are carried out in the cryostat with different surface treatments outside the cavities. Both of the cavities achieve stable continuous wave (CW) operation. A novel treatment, which cold-sprayed a 3.5 mm thick Cu layer onto the outside of the cavity, enables the maintenance of an average temperature of 5.5 K in the cavity at a RF loss of 10 W, implying that the thermal stability and uniformity of the cavity has been significantly improved. Through the synergistic control of four metal film resistors, a cooling rate of 0.06 K/min near 18 K is realized, and the cavity temperature gradient is reduced to 0.17 K/m, which effectively improves the RF performance of the cavity. The maximum $E_{acc}$ of the cavity reaches 3.42 MV/m, and the $Q_0$ is 1.1 × 10⁹. An electromagnetic–thermal coupling simulation model for the superconducting cavity is established and is in good agreement with the experimental results. The simulation results show that the cavity with a Cu-spraying treatment and the thermal links of 5N Al can satisfy the $E_{acc}$ of 10 MV/m under conduction cooling. Full article

Cultivated peanut (Arachis hypogaea L.) is a key oil- and protein-providing legume crop of the world. It is full of nutrients, and its nutrient profile is comparable to that of other nuts. Peanut is a unique plant as it showcases a pegging phenomenon, producing flowers above ground, and after fertilization, the developing peg enters the soil and produces seeds underground. This geocarpic nature of peanut exposes its seeds to soil pathogens. Peanut seeds are protected by an inedible pericarp and testa. The pericarp- and testa-specific promoters can be effectively used to improve the seed defense. We identified a pericarp- and testa-abundant expression gene (AhN8DT-2) from available transcriptome expression data, whose tissue-specific expression was further confirmed by the qRT-PCR. The 1827bp promoter sequence was used to construct the expression vector using the pMDC164 vector for further analysis. Quantitative expression of the GUS gene in transgenic Arabidopsis plants showed its high expression in the pericarp. GUS staining showed a deep blue color in the pericarp and testa. Cryostat sectioning of stained Arabidopsis seeds showed that expression is only limited to seed coat (testa), and staining was not present in cotyledons and embryos. GUS staining was not detected in any other tissues, including seedlings, leaves, stems, and roots, except for some staining in flowers. Under different phytohormones, this promoter did not show an increase in expression level. These results indicated that the AhN8DT-2 promoter drives GUS gene expression in a pericarp- and testa-specific manner. The identified promoter can be utilized to drive disease resistance genes, specifically in the pericarp and testa, enhancing peanut seed defense against soil-borne pathogens. This approach has broader implications for improving the resilience of peanut crops and other legumes, contributing to sustainable agricultural practices and food security. Full article