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Keywords = crop–livestock interactions

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28 pages, 1706 KiB  
Article
Adaptive Grazing and Land Use Coupling in Arid Pastoral China: Insights from Sunan County
by Bo Lan, Yue Zhang, Zhaofan Wu and Haifei Wang
Land 2025, 14(7), 1451; https://doi.org/10.3390/land14071451 - 11 Jul 2025
Viewed by 411
Abstract
Driven by climate change and stringent ecological conservation policies, arid and semi-arid pastoral areas face acute grassland degradation and forage–livestock imbalances. In Sunan County (Gansu Province, China), herders have increasingly turned to off-site grazing—leasing crop fields in adjacent oases during autumn and winter—to [...] Read more.
Driven by climate change and stringent ecological conservation policies, arid and semi-arid pastoral areas face acute grassland degradation and forage–livestock imbalances. In Sunan County (Gansu Province, China), herders have increasingly turned to off-site grazing—leasing crop fields in adjacent oases during autumn and winter—to alleviate local grassland pressure and adapt their livelihoods. However, the interplay between the evolving land use system (L) and this emergent borrowed pasture system (B) remains under-explored. This study introduces a coupled analytical framework linking L and B. We employ multi-temporal remote sensing imagery (2018–2023) and official statistical data to derive land use dynamic degree (LUDD) metrics and 14 indicators for the borrowed pasture system. Through entropy weighting and a coupling coordination degree model (CCDM), we quantify subsystem performance, interaction intensity, and coordination over time. The results show that 2017 was a turning point in grassland–bare land dynamics: grassland trends shifted from positive to negative, whereas bare land trends turned from negative to positive; strong coupling but low early coordination (C > 0.95; D < 0.54) were present due to institutional lags, infrastructural gaps, and rising rental costs; resilient grassroots networks bolstered coordination during COVID-19 (D ≈ 0.78 in 2023); and institutional voids limited scalability, highlighting the need for integrated subsidy, insurance, and management frameworks. In addition, among those interviewed, 75% (15/20) observed significant grassland degradation before adopting off-site grazing, and 40% (8/20) perceived improvements afterward, indicating its potential role in ecological regulation under climate stress. By fusing remote sensing quantification with local stakeholder insights, this study advances social–ecological coupling theory and offers actionable guidance for optimizing cross-regional forage allocation and adaptive governance in arid pastoral zones. Full article
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20 pages, 4690 KiB  
Article
Genome-Wide Characterization of VDAC Gene Family in Soybean (Glycine max L.) and In Silico Expression Profiling in Response to Drought and Salt Stress
by Muhammad Muneeb Ullah, Muqadas Aleem, Muhammad Mudassar Iqbal, Awais Riaz and Ainong Shi
Plants 2025, 14(14), 2101; https://doi.org/10.3390/plants14142101 - 8 Jul 2025
Viewed by 368
Abstract
Soybean (Glycine max L.) is grown worldwide to obtain edible oil, livestock feed, and biodiesel. However, drought and salt stress are becoming serious challenges to global soybean cultivation as they retard the growth of soybean plants and cause significant yield losses. Voltage-dependent [...] Read more.
Soybean (Glycine max L.) is grown worldwide to obtain edible oil, livestock feed, and biodiesel. However, drought and salt stress are becoming serious challenges to global soybean cultivation as they retard the growth of soybean plants and cause significant yield losses. Voltage-dependent anion-selective channel (VDAC) proteins are well-known for their role in drought and salt tolerance in crop plants. In this study, we identified 111 putative VDAC genes randomly distributed in genomes of 14 plant species, including cultivated soybean (Glycine max) and wild soybean (Glycine soja). The comparative phylogenetic studies classified these genes into six different clades and found the highest structural similarities among VDAC genes of G. max and G. soja. From the conserved domain database, porin-3 (PF01459) was found to be the conserved domain in all VDAC proteins. Furthermore, gene annotation studies revealed the role of GmaVDAC proteins in voltage-gated anion channel activity. These proteins were also found to interact with other proteins, especially mitochondrial receptors. A total of 103 miRNAs were predicted to target fifteen GmaVDAC genes. In G. max, these genes were found to be segmentally duplicated and randomly distributed on twelve chromosomes. Transcriptomic analysis revealed that the GmaVDAC18.2 gene showed overexpression in root nodules, whereas the GmaVDAC9.1, GmaVDAC18.1, and GmaVDAC18.2 genes showed overexpression under drought and salt stress conditions. Full article
(This article belongs to the Special Issue Functional Genomics and Molecular Breeding of Crops—2nd Edition)
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30 pages, 2837 KiB  
Review
Agriculture-Livestock-Forestry Nexus: Pathways to Enhanced Incomes, Soil Health, Food Security and Climate Change Mitigation in Sub-Saharan Africa
by Bonface O. Manono and Zipporah Gichana
Earth 2025, 6(3), 74; https://doi.org/10.3390/earth6030074 - 4 Jul 2025
Cited by 1 | Viewed by 1615
Abstract
Increasing global population and threat from climate change are imposing economic, social, and ecological challenges to global food production. The demand for food is increasing, necessitating enhanced agricultural production with minimal environmental impacts. To meet this demand, sustainable intensification of both crops and [...] Read more.
Increasing global population and threat from climate change are imposing economic, social, and ecological challenges to global food production. The demand for food is increasing, necessitating enhanced agricultural production with minimal environmental impacts. To meet this demand, sustainable intensification of both crops and livestock is necessary. This is more urgent in sub-Saharan Africa (SSA), a region characterized by low productivity and environmentally degrading agriculture. Integrated Agriculture-livestock-forestry (ALF) systems could be a key form of intensification needed for achieving food security and economic and environmental sustainability. The synergetic interactions between ALF nexus provide a mechanism to foster interconnectedness and resource circulation where practices of one system influence the outcomes in another. These systems enhance long-term farm sustainability while serving the farmers’ environmental and economic goals. It provides opportunities for improving food security, farmer incomes, soil health, climate resilience and the achievement of several UN Sustainable Development Goals. It is therefore crucial to strengthen the evidence supporting the contribution of these systems. On this basis, this paper reviews the potential pathways through which ALF nexus can enhance incomes, food security and climate change mitigation in SSA. The paper discusses the pathways through which the integration of crops, livestock and trees enhance (i) food security, (ii) incomes, (iii) soil health and (iv) mitigation of climate change in SSA. We argue that implementing ALF systems will be accompanied by an advancement of enhanced food security, farmer livelihoods and ecological conservation. It will foster a more balanced and sustainable sub-Saharan African agricultural systems. Full article
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22 pages, 919 KiB  
Article
The Grain Protein Content of Polish Cereals Other than Wheat: Can It Be Increased by Combining a Crop Sequence System, Cultivar Selection, and Plant Protection?
by Marta K. Kostrzewska and Magdalena Jastrzębska
Agriculture 2025, 15(9), 1016; https://doi.org/10.3390/agriculture15091016 - 7 May 2025
Viewed by 555
Abstract
After legumes, cereals are the most important source of protein for humans and livestock worldwide. One way to meet growing nutritional demands is to increase the grain protein content (GPC) of cereals. Breeding advances in this regard should be supported by optimized agricultural [...] Read more.
After legumes, cereals are the most important source of protein for humans and livestock worldwide. One way to meet growing nutritional demands is to increase the grain protein content (GPC) of cereals. Breeding advances in this regard should be supported by optimized agricultural practices. The GPCs of winter rye, winter triticale, spring barley, and spring oats grown in 2018–2022 in northeast Poland were evaluated to determine the influence of the crop sequence system (continuous monocropping, crop rotation), cultivar (two for each species), plant protection level (control treatment, herbicide, herbicide, and fungicide), and interactions among these factors. The cultivar selection was a significant GPC determinant in all cereals. Growing triticale in crop rotation after a legume increased its GPC compared to continuous monocropping, but decreased the GPC of rye and had no effect on the GPCs of spring cereal that followed non-legume crops. Using herbicides and herbicides combined with fungicides promoted the GPC of rye and oats, but not of triticale and barley. The heterogeneity of the interaction effects of the studied agricultural practices on the GPCs of the individual cereals prevents the identification of a universal combination that would ensure the highest GPC levels. The inter-annual weather variability played a significant role in shaping the GPCs of cereals and in modifying the influence of the controlled factors. Full article
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20 pages, 1009 KiB  
Article
Dryland Fodder Radish Genotypes: Planting Date Effects on Nutritive Value and In-Vitro Dry Matter Degradability in Midlands of KwaZulu-Natal, South Africa
by Lwando Mbambalala, Thamsanqa Doctor Empire Mpanza, Tlou Julius Tjelele, Lusanda Ncisana, Sphesihle Mkhungo, Lucky Sithole, Mpho Siennah Nzeru, Patrick Ngwako Rakau, Zikhona Theodora Rani-Kamwendo and Ntuthuko Raphael Mkhize
Grasses 2025, 4(2), 17; https://doi.org/10.3390/grasses4020017 - 1 May 2025
Cited by 1 | Viewed by 751
Abstract
Erratic rainfall and extended dry periods challenge forage production and livestock feed sustainability in dryland agriculture regions. This study investigated the effects of planting dates and genotype selection on the nutritive value and in-vitro dry matter degradability (IVDMD) of fodder radish genotypes in [...] Read more.
Erratic rainfall and extended dry periods challenge forage production and livestock feed sustainability in dryland agriculture regions. This study investigated the effects of planting dates and genotype selection on the nutritive value and in-vitro dry matter degradability (IVDMD) of fodder radish genotypes in Midlands of KwaZulu-Natal, South Africa. The experiment followed a completely randomised design with three fodder radish genotypes (Endurance, Line 2, and Nooitgedacht) and five planting dates (December, January, February, March and May). After three months of growth in each planting date, crops were harvested, prepared and analysed for various nutritional parameters including crude protein, fibre content, and IVDMD. Results revealed that December had the highest crude protein (28–31%) across genotypes, while March plantings optimised total non-structural carbohydrates (13.31%) and metabolisable energy (6.64 MJ/kg). The Nooitgedacht genotype demonstrated improved performance, achieving higher IVDMD of 85.54% for leaves in December plantings and 77.51% for tubers in February plantings. Significant interactions between planting dates and genotypes were observed for ash, crude protein, and cellulose in leaves. In conclusion, these findings highlight the crucial role of planting date selection and genotype choice in optimising fodder radish production under dryland conditions, offering valuable insights for enhancing livestock productivity and supporting sustainable rural livelihoods. Full article
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19 pages, 5004 KiB  
Article
Bio-Organic Fertilizer Application Enhances Silage Maize Yield by Regulating Soil Physicochemical and Microbial Properties
by Ying Tang, Lili Nian, Xu Zhao, Juan Li, Zining Wang and Liuwen Dong
Microorganisms 2025, 13(5), 959; https://doi.org/10.3390/microorganisms13050959 - 23 Apr 2025
Cited by 2 | Viewed by 883
Abstract
Silage maize is vital to livestock development in northern China, but intensive chemical fertilization has led to soil degradation and reduced productivity. Bio-organic fertilizers offer a sustainable alternative, though their effects on soil multifunctionality remain underexplored. This study evaluated the impact of combining [...] Read more.
Silage maize is vital to livestock development in northern China, but intensive chemical fertilization has led to soil degradation and reduced productivity. Bio-organic fertilizers offer a sustainable alternative, though their effects on soil multifunctionality remain underexplored. This study evaluated the impact of combining decomposed cow manure, Bacillus amyloliquefaciens, and mineral potassium fulvic acid with chemical fertilizers (NPK) on silage maize yield, soil microbial diversity, and ecosystem multifunctionality (EMF). Field experiments showed that bio-organic fertilization increased silage maize yield by 10.23% compared to chemical fertilizers alone, primarily by boosting labile organic carbon and soil enzyme activity. It also enhanced bacterial richness and diversity, with little effect on fungal communities. Microbial network analysis revealed more complex and stable bacterial networks under bio-organic treatments, indicating strengthened microbial interactions. Random forest and structural equation modeling (SEM) identified soil carbon storage and bacterial diversity as key drivers of EMF, which integrates soil functions such as nutrient cycling, decomposition, enzyme activity, and microbial diversity. These findings suggest that soil bacterial diversity and its interactions with soil properties are critical to both crop productivity and soil health. The optimal fertilization strategy for silage maize in this region involves the combined use of cattle manure, Bacillus amyloliquefaciens, mineral potassium fulvic acid, and NPK fertilizers. This approach improves yield, microbial diversity, and soil multifunctionality. Future studies should consider environmental variables and crop varieties across diverse regions to support broader application. Full article
(This article belongs to the Section Plant Microbe Interactions)
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17 pages, 986 KiB  
Review
Agroforestry for Food Security and Public Health: A Comprehensive Review
by Daniel Roberto Jung and Oduvaldo Vendrametto
Int. J. Environ. Res. Public Health 2025, 22(4), 645; https://doi.org/10.3390/ijerph22040645 - 19 Apr 2025
Viewed by 900
Abstract
Global food systems face mounting pressure from intersecting crises of food insecurity, malnutrition (affecting over 2.8 billion people), and climate change, necessitating transformative solutions. Agroforestry systems (AFS), integrating trees with crops and/or livestock, offer a promising pathway by synergistically enhancing food production, ecological [...] Read more.
Global food systems face mounting pressure from intersecting crises of food insecurity, malnutrition (affecting over 2.8 billion people), and climate change, necessitating transformative solutions. Agroforestry systems (AFS), integrating trees with crops and/or livestock, offer a promising pathway by synergistically enhancing food production, ecological stability, and public health outcomes. However, realizing this potential is hindered by gaps in understanding the complex interactions and trade-offs between these domains, limiting policy and practice effectiveness. This comprehensive review aimed to synthesize current evidence on how agroforestry integrates food security, public health, and environmental sustainability and to identify critical research gaps that limit its widespread adoption and optimization. Following the SPAR-4-SLR protocol, a systematic literature search was conducted across Web of Science and Scopus, with thematic analysis using VosViewer and quantitative synthesis of key metrics. The review confirms agroforestry’s multifaceted benefits, including enhanced dietary diversity, improved micronutrient intake (e.g., 18% reduction in vitamin A deficiency), significant carbon sequestration (0.5–2 Mg C/ha/year), soil health improvements (50–70% less erosion), income generation (+40%), and climate resilience (2–5 °C cooling). Key gaps identified include the need for longitudinal health studies, better quantification of climate–health interactions and non-material benefits, policy–health integration strategies, and analyses of economic–nutritional trade-offs. Full article
(This article belongs to the Section Environmental Health)
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20 pages, 3065 KiB  
Article
The Double-Edged Sword: Local Perspectives on the Spread, Impact, Management, and Uses of the Invasive Chromolaena odorata in Southern Nigeria
by Jane I. Otabor, Ikponmwosa Egbon, Michael D. Toews and Osariyekemwen Uyi
Sustainability 2025, 17(8), 3514; https://doi.org/10.3390/su17083514 - 14 Apr 2025
Viewed by 730
Abstract
In Nigeria, Chromolaena odorata poses significant threats to agriculture by disrupting ecosystem structure and function, thereby altering ecosystem services. However, our understanding of its impact, potential uses, and control measures, particularly from the perspective of local communities who interact directly with the plant, [...] Read more.
In Nigeria, Chromolaena odorata poses significant threats to agriculture by disrupting ecosystem structure and function, thereby altering ecosystem services. However, our understanding of its impact, potential uses, and control measures, particularly from the perspective of local communities who interact directly with the plant, presents opportunities for a balanced approach to sustainable management. The aim of this study was to document the knowledge and perceptions of the introduction and spread of C. odorata, its ethnopharmacological applications, and its effects on crops, livestock, and wildlife, as well as discuss control strategies. Using participatory rural appraisal techniques, we administered semi-structured questionnaires to 150 respondents across six villages in Edo State, Nigeria. The findings reveal that C. odorata is a well-recognized invasive species that has been present for several decades and is commonly referred to by its local name, “Awolowo weed”. Although many respondents were unsure of the reasons behind its introduction, most were familiar with its mode of dispersal. A significant proportion of respondents view the weed as a serious threat to agriculture and a major hindrance to human movement by foot to and from their farmlands where C odorata is dominant. However, many also reported its relative ease of management. Interestingly, 92.7% acknowledged the medicinal value of C. odorata, citing its use in treating fever, wounds, and stomach aches, while 84% reported its ability to enhance soil fertility. Most respondents did not perceive the plant as harmful to livestock or wildlife. Though many believe that the plant has continued to spread, 83.8% oppose its complete eradication. Instead, they highlighted Elephant grass (Pennisetum purpureum Schumach) as a more problematic weed that requires immediate intervention. In summary, exploring the local benefits of C. odorata highlights the importance of leveraging local knowledge and practices to develop a robust, integrated approach for its sustainable long-term management. Full article
(This article belongs to the Section Sustainability, Biodiversity and Conservation)
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18 pages, 6275 KiB  
Article
Evaluation of Dual-Purpose Triticale: Grain and Forage Productivity and Quality Under Semi-Arid Conditions
by Lei Cui, Linyuan Xu, Huihui Wang, Xiangtian Fan, Chahong Yan, Yanming Zhang, Changtong Jiang, Tong Zhou, Qing Guo, Yu Sun, Feng Yang and Hongjie Li
Agronomy 2025, 15(4), 881; https://doi.org/10.3390/agronomy15040881 - 31 Mar 2025
Cited by 1 | Viewed by 631
Abstract
Triticale (× Triticosecale Wittmack) is a valuable dual-purpose crop due to its adaptability to marginal environments and its potential for both high-quality grain and forage production. However, a comprehensive evaluation of its forage quality characteristics and agronomic performances is still needed. This study [...] Read more.
Triticale (× Triticosecale Wittmack) is a valuable dual-purpose crop due to its adaptability to marginal environments and its potential for both high-quality grain and forage production. However, a comprehensive evaluation of its forage quality characteristics and agronomic performances is still needed. This study evaluated the grain and forage yield potentials and nutritional compositions of 11 triticale genotypes over two consecutive years in a semi-arid region located in Shanxi province, China. Forage quality was assessed using several key parameters, including nutrient composition, fiber digestibility, mineral content, and energy density, while grain quality parameters, including nutrient composition as well as carbohydrate and fiber characteristics, were also analyzed. Significant genetic variation was observed in these traits, indicating the influence of genotype–environment interactions on these traits. The tested genotypes exhibited grain yields ranging from 4.83 to 6.92 t ha−1 and fresh forage biomass yields between 20.06 and 29.78 t ha−1, demonstrating their potential for sustainable forage and grain production under semi-arid conditions. Genotypes from our breeding programs, including Shengnongsicao 1 and Jinsicao 1, demonstrated superior adaptability, maintaining stable forage and grain yield potentials under adverse conditions. Their favorable nutritional characteristics further enhance their suitability for semi-arid livestock systems. High levels of essential minerals, particularly calcium and potassium, further enhanced the nutritional value of these genotypes. These results provide valuable insights for triticale breeding programs and suggest triticale’s potential as a reliable crop in semi-arid regions, where maximizing land productivity is essential. Full article
(This article belongs to the Special Issue Managing the Yield and Nutritive Value of Forage and Biomass Crops)
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17 pages, 5855 KiB  
Article
Effects of Exogenous Organic Matter on Soil Nutrient Dynamics and Its Role in Replacing Chemical Fertilizers for Vegetable Yield and Quality
by Juan Wang, Xinyue Li, Anquan Chen, Yan Li, Mengyun Xue and Shaoyuan Feng
Horticulturae 2024, 10(12), 1355; https://doi.org/10.3390/horticulturae10121355 - 17 Dec 2024
Cited by 2 | Viewed by 1148
Abstract
Searching for a low-cost soil amendment that can reduce the reliance on chemical fertilizers while maintaining crop yields is a vital issue to sustainable agricultural development. In this study, bio-organic matter derived from harmless disposal of livestock and poultry carcasses was used to [...] Read more.
Searching for a low-cost soil amendment that can reduce the reliance on chemical fertilizers while maintaining crop yields is a vital issue to sustainable agricultural development. In this study, bio-organic matter derived from harmless disposal of livestock and poultry carcasses was used to discuss its potential for substitute chemical fertilizer. An incubation experiment was conducted by incorporating bio-organic matter into the soil at the rate of 0% (CK), 1%, 2%, 3%, 4%, 5%, 6% and 7% (T1, T2, T3, T4, T5, T6, T7) of soil mass to investigate the effects of bio-organic matter on soil physical properties and the nutrient release dynamics. A pot experiment was conducted with three treatments: 150 mg·kg−1 nitrogen from compound fertilizer (CK), 150 mg·kg−1 nitrogen from bio-organic matter (B1) and 300 mg·kg−1 nitrogen from bio-organic matter (B2), to evaluate the potential of bio-organic matter as a substitute for chemical fertilizers in influencing the yield and quality of Chinese cabbage (Brassica chinensis L.). Results showed that in the incubation experiment, bio-organic matter addition reduced soil bulk density of 1.5% to 8.9% and increased soil porosity by ranging from 1.5% to 10.9%. The soil physical properties were significantly improved when addition rates ≥ 4% (by soil mass). The interaction effects of addition rate and incubation time had a significant effect on soil nutrients. In the pot experiment, substitution of chemical fertilizer with bio-organic matter did not reduce the yield, and the increasing application rate of bio-organic matter led to significantly higher soluble protein, soluble sugar and total phenol content of vegetables. Additionally, nitrite content in the vegetables was slightly lower with bio-organic matter compared to that under CK. It is concluded that bio-organic matter derived from the harmless disposal of livestock and poultry carcasses is feasible; it has great potential to partially or entirely replace chemical fertilizers, thereby contributing to realizing chemical fertilizer reduction. Full article
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25 pages, 3056 KiB  
Article
Impact of Nature Conservation Resources of Agroecology: Insights from Hungarian Farmers and Consumer Perspectives
by Annamária Harkányi and Apolka Ujj
Resources 2024, 13(12), 170; https://doi.org/10.3390/resources13120170 - 29 Nov 2024
Cited by 2 | Viewed by 1544
Abstract
This study investigated the implementation of agroecological principles on three organic farms in Hungary, focusing on four resource-focused, nature conservation-related agroecological basic elements identified by the FAO: biodiversity, interactions, recycling, and resilience. This research employed a mixed-methods approach, utilising in-depth interviews as a [...] Read more.
This study investigated the implementation of agroecological principles on three organic farms in Hungary, focusing on four resource-focused, nature conservation-related agroecological basic elements identified by the FAO: biodiversity, interactions, recycling, and resilience. This research employed a mixed-methods approach, utilising in-depth interviews as a technique to explore farmers’ practices and a questionnaire survey as a tool to assess consumer perspectives (with 63 respondents). The interviews facilitated a qualitative exploration of how agroecological practices are applied on farms, providing rich insights into the farmers’ experiences. Meanwhile, the questionnaire survey served as a structured instrument to measure consumer awareness and motivations concerning environmentally friendly farming methods. NVivo 12 software was employed for qualitative data analysis, assisting in coding and organizing responses to better understand recurring themes and patterns. The researchers found that all farms exhibited high biodiversity levels, facilitated through practices such as companion planting, crop rotation, and maintaining natural habitat patches. Agroecological farmers focus on practices suited to the landscape, fostering beneficial organisms and enhancing interactions between nature and agriculture. Integrating farm components (e.g., crops, livestock, water) promotes synergies that improve productivity and reduce reliance on external inputs. Recycling resources (like organic waste) within the farm increases efficiency, while resilience is strengthened through biodiversity, allowing farms to better withstand environmental stress. Direct marketing builds connections between producers and consumers, raising awareness of conservation practices. Consumer awareness regarding environmentally friendly agricultural practices was notably high, with findings indicating that health and ecological conservation motivations drive their purchasing decisions. This study highlights the context-dependent nature of agroecological practices, revealing that while implementation is robust, economic sustainability constraints may limit the extent to which all elements can be effectively applied. Since this research has certain limitations due to the limited sample size, expanding the study to include more farms would strengthen the findings. Nonetheless, these findings underscore the importance of integrating agroecological principles in organic farming to enhance biodiversity and foster sustainable agricultural practices. Full article
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18 pages, 5160 KiB  
Article
A Soybean Pyrroline-5-Carboxylate Dehydrogenase GmP5CDH1 Modulates Plant Growth and Proline Sensitivity
by Shupeng Dong, Zhuozhuo Mao, Zhongyi Yang, Xiao Li, Dezhou Hu, Fei Wu, Deyue Yu and Fang Huang
Agronomy 2024, 14(10), 2411; https://doi.org/10.3390/agronomy14102411 - 18 Oct 2024
Cited by 2 | Viewed by 1102
Abstract
Soybean [Glycine max (L.) Merr.], as a globally commercialized crop, is an important source of protein and oil for both humans and livestock. With more frequent extreme weather disasters, abiotic stress has become one of the critical factors restricting soybean production. Proline [...] Read more.
Soybean [Glycine max (L.) Merr.], as a globally commercialized crop, is an important source of protein and oil for both humans and livestock. With more frequent extreme weather disasters, abiotic stress has become one of the critical factors restricting soybean production. Proline (Pro) is a well-known substance in plants that responds to abiotic stress. To identify potential effector genes involved in soybean resistance to abiotic stress, we focused on the pyrroline-5-carboxylate dehydrogenase (P5CDH) which is a key enzyme in the degradation process of Pro. Through homologous sequence alignment, phylogenetic tree, and predicted expression, we chose GmP5CDH1 (Glyma.05G029200) for further research. Tissue-specific expression assay showed that GmP5CDH1 had higher expression levels in soybean seed and cotyledon development. Subcellular localization assay revealed that GmP5CDH1 was a nuclear-membrane-localized protein. As the result of the predicted cis-acting regulatory element indicates, the expression level of GmP5CDH1 was induced by low temperature, drought, salt stress, and ABA in soybean. Next, we constructed transgenic Arabidopsis overexpressing GmP5CDH1. The results showed that GmP5CDH1 also strongly responded to exogenous Pro, and overcame the toxicity of abiotic stress on plants by regulating the endogenous concentration of Pro. The interaction between GmP5CDH1 and GmSAM1 was validated through yeast two-hybrid, LUC fluorescence complementary, and BIFC. In conclusion, overexpression of a soybean pyrroline-5-carboxylate dehydrogenase GmP5CDH1 regulates the development of Arabidopsis thaliana by altering proline content dynamically under salt stress, especially improving the growth of plants under exogenous Pro. Full article
(This article belongs to the Special Issue Functional Genomics and Molecular Breeding of Soybeans)
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21 pages, 3825 KiB  
Article
Balancing Environmental Sustainability and Economic Viability in Luxembourgish Farms: An Agent-Based Model with Multi-Objective Optimization
by Alper Bayram, Antonino Marvuglia, Tomás Navarrete Gutiérrez and Hélène Soyeurt
Sustainability 2024, 16(19), 8536; https://doi.org/10.3390/su16198536 - 30 Sep 2024
Cited by 2 | Viewed by 1848
Abstract
To curb the impacts arising from the agricultural sector, the actions undertaken by policymakers, and ultimately by the farmers, are of paramount importance. However, finding the best strategy to reduce impacts, and especially assessing the effects of the interactions and mutual influence among [...] Read more.
To curb the impacts arising from the agricultural sector, the actions undertaken by policymakers, and ultimately by the farmers, are of paramount importance. However, finding the best strategy to reduce impacts, and especially assessing the effects of the interactions and mutual influence among farmers, is very difficult. To this aim, this paper shows an application of an agent-based model (ABM) coupled with life cycle assessment (LCA), which also includes multi-objective optimization of farming activities (including both crop cultivation and livestock breeding) from an economic and environmental perspective. The environmental impacts are assessed using the impact assessment scores calculated with the Environmental Footprint 3.0 life cycle impact assessment method and the study is conducted “from cradle to farm gate”. The model is applied to all the farms in Luxembourg, whose network is built utilizing neighborhood interactions, through which a parameter known as farmer’s green consciousness is updated at each time step. The optimization module is instantiated at the end of each time step, and decision variables (the number of livestock units and land allocation) are assigned based on profitability and specified environmental impact categories. If only profit optimization is considered (i.e., when farmers’ green consciousness is de-activated), the results show a 9% reduction in the aggregated environmental impacts (obtained as the Environmental Footprint single score) and a 5.5% increase in overall profitability. At the farm level, simulations display a clear trade-off between environmental sustainability and financial stability, with a 25% reduction in overall emissions possible if farming activities are carried out using the single score impact in the objective function, though this results in an 8% reduction in profitability over 10 years. Full article
(This article belongs to the Special Issue Sustainable Crop Production and Agricultural Practices)
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21 pages, 5176 KiB  
Article
Combining Drone LiDAR and Virtual Reality Geovisualizations towards a Cartographic Approach to Visualize Flooding Scenarios
by Ermioni Eirini Papadopoulou and Apostolos Papakonstantinou
Drones 2024, 8(8), 398; https://doi.org/10.3390/drones8080398 - 15 Aug 2024
Cited by 5 | Viewed by 3048
Abstract
This study aims to create virtual reality (VR) geovisualizations using 3D point clouds obtained from airborne LiDAR technology. These visualizations were used to map the current state of river channels and tributaries in the Thessalian Plain, Greece, following severe flooding in the summer [...] Read more.
This study aims to create virtual reality (VR) geovisualizations using 3D point clouds obtained from airborne LiDAR technology. These visualizations were used to map the current state of river channels and tributaries in the Thessalian Plain, Greece, following severe flooding in the summer of 2023. The study area examined in this paper is the embankments enclosing the tributaries of the Pineios River in the Thessalian Plain region, specifically between the cities of Karditsa and Trikala in mainland Greece. This area was significantly affected in the summer of 2023 when flooding the region’s rivers destroyed urban elements and crops. The extent of the impact across the entire Thessalian Plain made managing the event highly challenging to the authorities. High-resolution 3D mapping and VR geovisualization of the embarkments encasing the main rivers and the tributaries of the Thessalian Plain essentially provides information for planning the area’s restoration processes and designing prevention and mitigation measures for similar disasters. The proposed methodology consists of four stages. The first and second stages of the methodology present the design of the data acquisition process with airborne LiDAR, aiming at the high-resolution 3D mapping of the sites. The third stage focuses on data processing, cloud point classification, and thematic information creation. The fourth stage is focused on developing the VR application. The VR application will allow users to immerse themselves in the study area, observe, and interact with the existing state of the embankments in high resolution. Additionally, users can interact with the 3D point cloud, where thematic information is displayed describing the classification of the 3D cloud, the altitude, and the RGB color. Additional thematic information in vector form, providing qualitative characteristics, is also illustrated in the virtual space. Furthermore, six different scenarios were visualized in the 3D space using a VR app. Visualizing these 3D scenarios using digital twins of the current antiflood infrastructure provides scenarios of floods at varying water levels. This study aims to explore the efficient visualization of thematic information in 3D virtual space. The goal is to provide an innovative VR tool for managing the impact on anthropogenic infrastructures, livestock, and the ecological capital of various scenarios of a catastrophic flood. Full article
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13 pages, 585 KiB  
Article
Soil and Foliar Zinc Biofortification of Triticale (x Triticosecale) under Mediterranean Conditions: Effects on Forage Yield and Quality
by Carlos García-Latorre, María Dolores Reynolds-Marzal, Saúl De la Peña-Lastra, Nuno Pinheiro and María José Poblaciones
Plants 2024, 13(14), 1917; https://doi.org/10.3390/plants13141917 - 11 Jul 2024
Cited by 3 | Viewed by 1596
Abstract
Zinc (Zn) deficiency represents a significant global concern, affecting both plant and human health, particularly in regions with Zn-depleted soils. Agronomic biofortification strategies, such as the application of Zn fertilizers, offer a cost-effective approach to increase Zn levels in crops. This study aimed [...] Read more.
Zinc (Zn) deficiency represents a significant global concern, affecting both plant and human health, particularly in regions with Zn-depleted soils. Agronomic biofortification strategies, such as the application of Zn fertilizers, offer a cost-effective approach to increase Zn levels in crops. This study aimed to assess the efficacy of soil and foliar Zn biofortification, applied as an aqueous solution of 0.5% zinc sulphate (ZnSO4·7H2O), on triticale (x Triticosecale) grown under Mediterranean conditions. The study was conducted over two growing seasons (2017/18 and 2018/19) in southern Spain, evaluating the effects on biomass yield; forage quality, including crude protein, Van Soest detergent fiber, organic matter digestibility, and relative forage value; and nutrient accumulation. Soil treatment consisted in the application of 50 kg of ZnSO4·7H2O ha−1 solely at the beginning of the first campaign to assess the residual effect on the second year. In contrast, the foliar treatment consisted of two applications of 4 kg of ZnSO4·7H2O ha−1 per campaign, one at the beginning of tillering and the other at the appearance of the first node. The foliar application increased the Zn content of the forage to adequate levels, while the soil application resulted in a 33% increase in biomass production, which is particularly beneficial for farmers. Overall quality was favored by the combined soil + foliar application, and no adverse antagonistic effects on other nutrients were detected. Instead, a synergistic interaction between Se and Zn was observed, which improved the efficacy of this important micronutrient for livestock and human wellbeing. Full article
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