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21 pages, 4924 KB  
Article
CB-YOLOv7: A Modified YOLOv7 Approach for Accurate Weed Detection in Complex UAV Imagery from Cotton Fields
by Anindita Das, Yong Yang and Vinitha Hannah Subburaj
AgriEngineering 2026, 8(6), 235; https://doi.org/10.3390/agriengineering8060235 - 11 Jun 2026
Viewed by 276
Abstract
Weed detection is an important part of precision agriculture because it allows farmers to manage weeds more efficiently and reduce unnecessary herbicide use. With the use of UAVs, it is now possible to capture high-resolution images of agricultural fields, but identifying weeds from [...] Read more.
Weed detection is an important part of precision agriculture because it allows farmers to manage weeds more efficiently and reduce unnecessary herbicide use. With the use of UAVs, it is now possible to capture high-resolution images of agricultural fields, but identifying weeds from these images is still challenging due to complex backgrounds, lighting variations, and the visual similarity between crops and weeds. In this study, an improved YOLOv7-based approach is developed to address these challenges using UAV imagery collected from rainfed cotton fields in the Texas Panhandle. The original dataset consisted of high-resolution UAV images, which were divided into smaller patches and manually annotated to label weed and cotton classes. After cleaning the dataset and applying simple augmentation techniques, a total of 8396 images were used for training and testing. To improve detection performance, two modifications were introduced: Convolutional Block Attention Module (CBAM) to help the model focus on important features and Bidirectional Feature Pyramid Network (BiFPN) to improve how information is shared across different scales. Three models—YOLOv7-CBAM, YOLOv7-BiFPN, and the combined CB-YOLOv7—were evaluated. The results show that CBAM helps detect more weed instances, BiFPN reduces false detections, and the combined model gives the best overall performance, achieving an mAP@0.5 of 0.89 and an F1-score of 0.84. Overall, the study shows that improving both the dataset and the model can lead to more reliable weed detection under real field conditions. The proposed approach can be useful for identifying weeds in cotton fields using UAV imagery and can support better crop management and more efficient use of herbicides in precision agriculture. Full article
(This article belongs to the Special Issue Applications of Computer Vision in Agriculture)
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20 pages, 11396 KB  
Article
Development of a Robotic Weed Puller for Precision Management of Palmer Amaranth in Cotton
by Taranjeet Singh Sodhi, Shekhar Thapa, Canicius Mwitta and Glen C. Rains
AgriEngineering 2026, 8(6), 226; https://doi.org/10.3390/agriengineering8060226 - 5 Jun 2026
Viewed by 564
Abstract
The objective of this study was to design, fabricate, and test an automated inter-row robotic system for the precision management of Palmer amaranth (Amaranthus palmeri) in cotton. A Farm-ng robotic platform with custom-designed weed pulling and cutting attachments was used to [...] Read more.
The objective of this study was to design, fabricate, and test an automated inter-row robotic system for the precision management of Palmer amaranth (Amaranthus palmeri) in cotton. A Farm-ng robotic platform with custom-designed weed pulling and cutting attachments was used to achieve weed control. The pulling system consisted of two counter-rotating rollers with a frictional cover to uproot weeds, followed by a cutting operation to shred the weeds into smaller pieces, preventing regrowth. A deep learning model, YOLOv11s, was used for weed identification, while point cloud data from a stereo camera was used to estimate weed height in real-time for dynamic adjustment of the puller height. The system was evaluated at three forward speeds (0.06, 0.15, and 0.25 m/s), two roller speeds (107 and 161 RPM), and three attachment configurations (puller-only, cutter-only, and combined). The combined configuration consistently outperformed individual operations, achieving 80% control at 0.15 m/s and a roller speed of 161 RPM. Optimal performance was observed when the angular puller velocity was 15–25 times the forward speed of the rover. This approach demonstrates the potential of integrating mechanical weed removal with real-time computer vision to improve weed management and reduce labor requirements. Full article
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15 pages, 2928 KB  
Article
ES2-LeafSeg: Lightweight State Space Modeling-Driven Agricultural Leaf Segmentation
by Hao Wang, Zhiyang Li, Pengsen Zhao and Jinlong Yu
Appl. Sci. 2026, 16(8), 3745; https://doi.org/10.3390/app16083745 - 10 Apr 2026
Viewed by 423
Abstract
Agricultural robots and unmanned farmland management require real-time and precise parsing of crop leaves at the edge to support variable application of pesticides, seedling condition monitoring, and phenotypic analysis. However, the field environment features drastic changes in light, leaf occlusion, and interference from [...] Read more.
Agricultural robots and unmanned farmland management require real-time and precise parsing of crop leaves at the edge to support variable application of pesticides, seedling condition monitoring, and phenotypic analysis. However, the field environment features drastic changes in light, leaf occlusion, and interference from background weeds, which can cause semantic fragmentation and boundary artifacts in lightweight models. This paper presents ES2-LeafSeg, a lightweight framework for leaf semantic segmentation tailored for edge deployment. The method employs EfficientNetV2 as the backbone encoder and introduces the State Space Semantic Enhancement Module (S2FEM) on skip connection features, modeling long-range dependencies and suppressing local texture noise through SSM pooling in row and column directions. Meanwhile, a cross-scale decoder (CSD) and a global context transformation (GCT) are designed to achieve multi-scale semantic fusion and boundary refinement. On the three-class segmentation task of the SoyCotton dataset, ES2-LeafSeg achieved mIoU of 0.817, mDice of 0.869, Fβw of 0.925, and MAE of 0.011, outperforming multiple classic and recent baselines while maintaining 23.67 M parameters and 49.62 FPS. Ablation experiments further verified the complementary contributions of S2FEM and GCT to regional consistency and boundary quality. Full article
(This article belongs to the Section Computing and Artificial Intelligence)
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29 pages, 11160 KB  
Article
AVGS-YOLO: A Quad-Synergistic Lightweight Enhanced YOLOv11 Model for Accurate Cotton Weed Detection in Complex Field Environments
by Suqi Wang and Linjing Wei
Agriculture 2026, 16(8), 828; https://doi.org/10.3390/agriculture16080828 - 8 Apr 2026
Cited by 1 | Viewed by 725
Abstract
Cotton represents one of the world’s most significant agricultural commodities. However, severe weed proliferation in cotton fields seriously hampers the development of the cotton industry, making precise weed control essential for ensuring healthy cotton growth. Traditional object detection methods often suffer from computational [...] Read more.
Cotton represents one of the world’s most significant agricultural commodities. However, severe weed proliferation in cotton fields seriously hampers the development of the cotton industry, making precise weed control essential for ensuring healthy cotton growth. Traditional object detection methods often suffer from computational complexity, rendering them difficult to deploy on resource-constrained edge devices. To address this challenge, this paper proposes AVGS-YOLO, a lightweight and enhanced model employing a Quadruple Synergistic Lightweight Perception Mechanism (QSLPM) for precise weed detection in complex cotton field environments. The QSLPM emphasizes synergistic interactions between modules. It integrates lightweight neck architecture (Slimneck) to optimize feature extraction pathways for cotton weeds; the ADown module (Adaptive Downsampling) replaces Conv modules to address model parameter redundancy; the small object attention modulation module (SEAM) enhances the recognition of small-scale cotton weed features; and angle-sensitive geometric regression (SIoU) improves bounding box localization accuracy. Experimental results demonstrate that the AVGS-YOLO model achieves 95.9% precision, 94.2% recall, 98.2% mAP50, and 93.3% mAP50-95. While maintaining high detection accuracy, the model achieves a lightweight design with reductions of 17.4% in parameters, 27% in GFLOPs, and 14.5% in model size. Demonstrating strong performance in identifying cotton weeds within complex cotton field environments, this model provides technical support for deployment on resource-constrained edge devices, thereby advancing intelligent agricultural development and safeguarding the healthy growth of cotton crops. Full article
(This article belongs to the Section Artificial Intelligence and Digital Agriculture)
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28 pages, 48517 KB  
Article
DDF-DETR: A Multi-Scale Spatial Context Method for Field Cotton Seedling Detection
by Feng Xu, Huade Zhou, Yinyi Pan, Yi Lu and Luan Dong
Agriculture 2026, 16(5), 615; https://doi.org/10.3390/agriculture16050615 - 7 Mar 2026
Viewed by 810
Abstract
Accurate assessment of cotton emergence rates is essential for precision agriculture management, and unmanned aerial vehicle (UAV) imagery provides a scalable means for field-level monitoring. However, cotton seedling detection from UAV images faces persistent challenges: individual seedlings appear as small targets with diverse [...] Read more.
Accurate assessment of cotton emergence rates is essential for precision agriculture management, and unmanned aerial vehicle (UAV) imagery provides a scalable means for field-level monitoring. However, cotton seedling detection from UAV images faces persistent challenges: individual seedlings appear as small targets with diverse morphologies across varying flight altitudes; strong plastic film reflections, weeds, and soil cracks introduce substantial background interference; and “missing seedling” targets, which manifest as negative space features, exhibit high similarity to background noise. Existing CNN–Transformer hybrid detection architectures are limited by fixed convolutional receptive fields that cannot adapt to multi-scale target variations, attention mechanisms that lack explicit directional geometric modeling, and interpolation-based upsampling that attenuates high-frequency edge details of small targets. To address these issues, this paper proposes DDF-DETR (Dynamic-Direction-Frequency Detection Transformer), a multi-scale spatial context detection method based on RT-DETR. The method incorporates three components: a Dynamic Gated Mixer Block (DGMB) for adaptive multi-scale feature extraction with background noise suppression, a Direction-Aware Adaptive Transformer Encoder (DAATE) for directional geometric feature modeling at linear computational complexity, and a Frequency-Aware Sub-pixel Upsampling Network (FASN) for high-frequency detail recovery in the feature pyramid. On the self-constructed Xinjiang cotton field dataset, DDF-DETR achieves 83.72% mAP@0.5 and 63.46% mAP@0.5:0.95, representing improvements of 2.38% and 5.28% over the baseline RT-DETR-R18, while reducing the parameter count by 30.6% and computational cost to 42.8 GFLOPs. Generalization experiments on the VisDrone2019 and TinyPerson datasets further validate the robustness of the proposed method for small target detection across different scenarios. Full article
(This article belongs to the Section Artificial Intelligence and Digital Agriculture)
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18 pages, 4205 KB  
Article
Research on Field Weed Target Detection Algorithm Based on Deep Learning
by Ziyang Chen, Le Wu, Zhenhong Jia, Jiajia Wang, Gang Zhou and Zhensen Zhang
Sensors 2026, 26(2), 677; https://doi.org/10.3390/s26020677 - 20 Jan 2026
Cited by 1 | Viewed by 617
Abstract
Weed detection algorithms based on deep learning are considered crucial for smart agriculture, with the YOLO series algorithms being widely adopted due to their efficiency. However, existing YOLO algorithms struggle to maintain high accuracy, while low parameter requirements and computational efficiency are achieved [...] Read more.
Weed detection algorithms based on deep learning are considered crucial for smart agriculture, with the YOLO series algorithms being widely adopted due to their efficiency. However, existing YOLO algorithms struggle to maintain high accuracy, while low parameter requirements and computational efficiency are achieved when weeds with occlusion or overlap are detected. To address this challenge, a target detection algorithm called SSS-YOLO based on YOLOv9t is proposed in this paper. First, the SCB (Spatial Channel Conv Block) module is introduced, in which large kernel convolution is employed to capture long-range dependencies, occluded weed regions are bypassed by being associated with unobstructed areas, and features of unobstructed regions are enhanced through inter-channel relationships. Second, the SPPF EGAS (Spatial Pyramid Pooling Fast Edge Gaussian Aggregation Super) module is proposed, where multi-scale max pooling is utilized to extract hierarchical contextual features, large receptive fields are leveraged to acquire background information around occluded objects, and features of weed regions obscured by crops are inferred. Finally, the EMSN (Efficient Multi-Scale Spatial-Feedforward Network) module is developed, through which semantic information of occluded regions is reconstructed by contextual reasoning and background vegetation interference is effectively suppressed while visible regional details are preserved. To validate the performance of this method, experiments are conducted on both our self-built dataset and the publicly available Cotton WeedDet12 dataset. The results demonstrate that compared to existing algorithms, significant performance improvements are achieved by the proposed method. Full article
(This article belongs to the Section Smart Agriculture)
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22 pages, 84914 KB  
Article
GEFA-YOLO: Lightweight Weed Detection with Group-Enhanced Fusion Attention
by Huicheng Li, Pushi Zhao, Feng Kang, Yuting Su, Qi Zhou, Zhou Wang and Lijin Wang
Sensors 2026, 26(2), 540; https://doi.org/10.3390/s26020540 - 13 Jan 2026
Viewed by 926
Abstract
Cotton is an important economic crop, and its weed management directly affects yield and quality. In actual cotton fields, detection accuracy still faces challenges due to the complex types of weeds, variable morphologies, and environmental factors. Most existing models rely on the attention [...] Read more.
Cotton is an important economic crop, and its weed management directly affects yield and quality. In actual cotton fields, detection accuracy still faces challenges due to the complex types of weeds, variable morphologies, and environmental factors. Most existing models rely on the attention mechanism to improve performance, but channel attention tends to ignore spatial information, while full spatial attention brings high computational costs. Therefore, this paper proposes a grouped enhanced fusion attention mechanism (GEFA), which combines grouped convolution and local spatial attention to reduce complexity and parameter quantity while effectively enhancing feature expression ability. The GEFAY detection model constructed based on GEFA achieves good balance in efficiency, accuracy, and complexity on the CottonWeedDet12, VOC, and COCO datasets. Compared with classic attention methods, this model has the smallest increase in parameters and computational costs while significantly improving accuracy. It is more suitable for deployment on edge devices. The further designed end-to-end intelligent weed detection system and edge device deployment can achieve image detection on local maps and real-time cameras, with good practicality and scalability, providing effective technical support for intelligent visual applications in precision agriculture. Full article
(This article belongs to the Section Smart Agriculture)
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21 pages, 2670 KB  
Article
Analysis of Photosynthetic Parameters, Yield, and Quality Correlations in Herbicide-Tolerant Transgenic Hybrid Cotton
by Ping He, Meiqi Liu, Haoyu Jiang, Zexing Zhang, Zitang Bian, Yongqiang Liu, Honglei Ma, Jianbo Zhu, Tianqi Jiao and Ruina Liu
Int. J. Mol. Sci. 2026, 27(1), 400; https://doi.org/10.3390/ijms27010400 - 30 Dec 2025
Cited by 1 | Viewed by 611
Abstract
Weed stress remains a major limiting factor in cotton production, and glyphosate-tolerant varieties provide an effective solution for chemical weed control. However, achieving a balance between herbicide tolerance and agronomic physiological traits remains challenging. In this study, three hybrid combinations were generated by [...] Read more.
Weed stress remains a major limiting factor in cotton production, and glyphosate-tolerant varieties provide an effective solution for chemical weed control. However, achieving a balance between herbicide tolerance and agronomic physiological traits remains challenging. In this study, three hybrid combinations were generated by crossing a glyphosate-tolerant cotton line (GGK2) with conventional elite lines and were comprehensively evaluated. Gene expression analysis revealed that the classical detoxification gene GAT was significantly downregulated in all hybrid combinations, whereas the expression of GR79-EPSPS, a gene associated with glutathione metabolism and oxidative stress response, was markedly elevated, particularly in the GGK2 × Y4 combination. This differential expression pattern suggests that GR79-EPSPS may compensate for the reduced function of GAT by conferring oxidative protection under herbicide stress. Physiological determination indicated that hybrid combinations with enhanced GR79-EPSPS expression, especially GGK2 × Y5, exhibited superior photosynthetic pigment composition and photosystem II (PSII) efficiency, validating the role of GR79-EPSPS in maintaining photosynthetic stability. Agronomic trait assessment demonstrated that GGK2 × Y4 achieved significant biomass accumulation and yield improvement through heterosis, although fiber quality improvement was limited. This study effectively enhanced the herbicide resistance of conventional cotton through crossbreeding and revealed that the interaction between GR79-EPSPS and GAT can improve cotton tolerance to herbicides, thereby providing a breeding strategy for developing cotton varieties with both herbicide tolerance and superior agronomic traits. Full article
(This article belongs to the Special Issue 25th Anniversary of IJMS: Updates and Advances in Molecular Biology)
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18 pages, 14117 KB  
Article
Benchmarking YOLO Models for Crop Growth and Weed Detection in Cotton Fields
by Hassan Raza, Muhammad Abu Bakr, Sultan Daud Khan, Hira Batool, Habib Ullah and Mohib Ullah
AgriEngineering 2025, 7(11), 375; https://doi.org/10.3390/agriengineering7110375 - 5 Nov 2025
Cited by 22 | Viewed by 2651
Abstract
Reliable differentiation of crops and weeds is essential for precision agriculture, where real-time detection can minimize chemical inputs and support site-specific interventions. This study presents the large-scale and systematic benchmark of 19 YOLO-family variants, spanning YOLOv3 through YOLOv11, for cotton–weed detection using the [...] Read more.
Reliable differentiation of crops and weeds is essential for precision agriculture, where real-time detection can minimize chemical inputs and support site-specific interventions. This study presents the large-scale and systematic benchmark of 19 YOLO-family variants, spanning YOLOv3 through YOLOv11, for cotton–weed detection using the Cotton–8 dataset. The dataset comprises 4440 annotated field images with five categories: broadleaf weeds, grass weeds, and three growth stages of cotton. All models were trained under a standardized protocol with COCO-pretrained weights, fixed seeds, and Ultralytics implementations to ensure reproducibility and fairness. Inference was conducted with a confidence threshold of 0.25 and a non-maximum suppression (NMS) IoU threshold of 0.45, with test-time augmentation (TTA) disabled. Evaluation employed precision, recall, mAP@0.5, and mAP@0.5:0.95, along with inference latency and parameter counts to capture accuracy–efficiency trade-offs. Results show that larger models, such as YOLO11x, achieved the best detection accuracy (mAP@0.5 = 81.5%), whereas lightweight models like YOLOv8n and YOLOv9t offered the fastest inference ( 27 msper image) but with reduced accuracy. Across classes, cotton growth stages were detected reliably, but broadleaf and grass weeds remained challenging, especially under stricter localization thresholds. These findings highlight that the key bottleneck lies in small-object detection and precise localization rather than architectural design. By providing the first direct comparison across successive YOLO generations for weed detection in cotton, this work offers a practical reference for researchers and practitioners selecting models for real-world, resource-constrained cotton–weed management. Full article
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13 pages, 525 KB  
Review
Weed Resistance to Herbicides in Mexico: A Review
by José Alfredo Domínguez-Valenzuela, Candelario Palma-Bautista, Román Eleazar Ruiz-Romero, José G. Vázquez-García, Juan Carlos Delgado-Castillo, Hugo E. Cruz-Hipólito, Ricardo Alcántara-de la Cruz, Rafael De Prado and Guido Plaza
Agronomy 2025, 15(10), 2411; https://doi.org/10.3390/agronomy15102411 - 17 Oct 2025
Cited by 4 | Viewed by 2266
Abstract
Herbicide resistance in weeds has become a critical challenge in worldwide and Mexican agriculture. Many of these cases involve single, cross and multiple resistance to herbicides that inhibit Acetyl CoA Carboxylase (ACCase), Acetolactate Synthase (ALS), Hydroxyphenyl Pyruvate Dioxygenase (HPPD), and Enolpyruvyl Shikimate Phosphate [...] Read more.
Herbicide resistance in weeds has become a critical challenge in worldwide and Mexican agriculture. Many of these cases involve single, cross and multiple resistance to herbicides that inhibit Acetyl CoA Carboxylase (ACCase), Acetolactate Synthase (ALS), Hydroxyphenyl Pyruvate Dioxygenase (HPPD), and Enolpyruvyl Shikimate Phosphate Synthase (EPSPS) enzymes, as well as auxin mimic herbicides. Documented resistance mechanisms include both target-site resistance (TSR) mutations and various forms of non-target-site resistance (NTSR). In wheat and barley, biotypes with resistance to ACCase, ALS, EPSPS and auxins have been confirmed. Maize–sorghum systems show resistance to ACCase, ALS and EPSPS, and in cotton there are glyphosate-resistant populations of Amaranthus palmeri. Citrus orchards remain the focus of glyphosate resistance. Of concern is the advance of multiple resistance in cereals, exemplified by Avena fatua (ACCase + ALS) and Brassica rapa (EPSPS + ALS + auxin mimics). Unique cases, such as EPSPS resistance in Leptochloa virgata and Bidens pilosa and to HPPD in Setaria adhaerens, are unique to Mexico. These resistance patterns underline the need for robust monitoring and detailed study of molecular and physiological mechanisms, where this has not been done, to inform integrated weed management strategies and curb the spread of weeds. Full article
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22 pages, 29892 KB  
Article
Lightweight Deep Learning for Real-Time Cotton Monitoring: UAV-Based Defoliation and Boll-Opening Rate Assessment
by Minghui Xia, Xuegeng Chen, Xinliang Tian, Haojun Wen, Yan Zhao, Hongxia Liu, Wei Liu and Yuchen Zheng
Agriculture 2025, 15(19), 2095; https://doi.org/10.3390/agriculture15192095 - 8 Oct 2025
Cited by 3 | Viewed by 1298
Abstract
Unmanned aerial vehicle (UAV) imagery provides an efficient approach for monitoring cotton defoliation and boll-opening rates. Deep learning, particularly convolutional neural networks (CNNs), has been widely applied in image processing and agricultural monitoring, achieving strong performance in tasks such as disease detection, weed [...] Read more.
Unmanned aerial vehicle (UAV) imagery provides an efficient approach for monitoring cotton defoliation and boll-opening rates. Deep learning, particularly convolutional neural networks (CNNs), has been widely applied in image processing and agricultural monitoring, achieving strong performance in tasks such as disease detection, weed recognition, and yield prediction. However, existing models often suffer from heavy computational costs and slow inference speed, limiting their real-time deployment in agricultural fields. To address this challenge, we propose a lightweight cotton maturity recognition model, RTCMNet (Real-time Cotton Monitoring Network). By incorporating a multi-scale convolutional attention (MSCA) module and an efficient feature fusion strategy, RTCMNet achieves high accuracy with substantially reduced computational complexity. A UAV dataset was constructed using images collected in Xinjiang, and the proposed model was benchmarked against several state-of-the-art networks. Experimental results demonstrate that RTCMNet achieves 0.96 and 0.92 accuracy on defoliation rate and boll-opening rate classification tasks, respectively. Meanwhile, it contains only 0.35 M parameters—94% fewer than DenseNet121—and only requires an inference time of 33 ms, representing a 97% reduction compared to DenseNet121. Field tests further confirm its real-time performance and robustness on UAV platforms. Overall, RTCMNet provides an efficient and low-cost solution for UAV-based cotton maturity monitoring, supporting the advancement of precision agriculture. Full article
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17 pages, 6432 KB  
Article
An AI-Enabled System for Automated Plant Detection and Site-Specific Fertilizer Application for Cotton Crops
by Arjun Chouriya, Peeyush Soni, Abhilash K. Chandel and Ajay Kumar Patel
Automation 2025, 6(4), 53; https://doi.org/10.3390/automation6040053 - 8 Oct 2025
Cited by 1 | Viewed by 1902
Abstract
Typical fertilizer applicators are often restricted in performance due to non-uniformity in distribution, required labor and time intensiveness, high discharge rate, chemical input wastage, and fostering weed proliferation. To address this gap in production agriculture, an automated variable-rate fertilizer applicator was developed for [...] Read more.
Typical fertilizer applicators are often restricted in performance due to non-uniformity in distribution, required labor and time intensiveness, high discharge rate, chemical input wastage, and fostering weed proliferation. To address this gap in production agriculture, an automated variable-rate fertilizer applicator was developed for the cotton crop that is based on deep learning-initiated electronic control unit (ECU). The applicator comprises (a) plant recognition unit (PRU) to capture and predict presence (or absence) of cotton plants using the YOLOv7 recognition model deployed on-board Raspberry Pi microprocessor (Wale, UK), and relay decision to a microcontroller; (b) an ECU to control stepper motor of fertilizer metering unit as per received cotton-detection signal from the PRU; and (c) fertilizer metering unit that delivers precisely metered granular fertilizer to the targeted cotton plant when corresponding stepper motor is triggered by the microcontroller. The trials were conducted in the laboratory on a custom testbed using artificial cotton plants, with the camera positioned 0.21 m ahead of the discharge tube and 16 cm above the plants. The system was evaluated at forward speeds ranging from 0.2 to 1.0 km/h under lighting levels of 3000, 5000, and 7000 lux to simulate varying illumination conditions in the field. Precision, recall, F1-score, and mAP of the plant recognition model were determined as 1.00 at 0.669 confidence, 0.97 at 0.000 confidence, 0.87 at 0.151 confidence, and 0.906 at 0.5 confidence, respectively. The mean absolute percent error (MAPE) of 6.15% and 9.1%, and mean absolute deviation (MAD) of 0.81 g/plant and 1.20 g/plant, on application of urea and Diammonium Phosphate (DAP), were observed, respectively. The statistical analysis showed no significant effect of the forward speed of the conveying system on fertilizer application rate (p > 0.05), thereby offering a uniform application throughout, independent of the forward speed. The developed fertilizer applicator enhances precision in site-specific applications, minimizes fertilizer wastage, and reduces labor requirements. Eventually, this fertilizer applicator placed the fertilizer near targeted plants as per the recommended dosage. Full article
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13 pages, 3420 KB  
Article
Design, Synthesis and Herbicidal Activity of 1,2,4-Oxadiazole Compounds as Novel Light-Dependent Protochlorophyllide Oxidoreductase Inhibitors
by Xiao Hu, Jing Miao, Yiyi Tian, Wennan Luo, Jixian Shang, Ruiyuan Liu and Huizhe Lu
Molecules 2025, 30(19), 3970; https://doi.org/10.3390/molecules30193970 - 3 Oct 2025
Cited by 2 | Viewed by 1249
Abstract
Light-dependent protochlorophyllide oxidoreductase (LPOR, E.C.1.3.1.33) plays a crucial role in the biosynthesis of chlorophyll in plants. Therefore, inactivating LPOR can hinder the production of chlorophyll to achieve the effect of weed control. In this research, utilizing an active substructure splicing method, 20 new [...] Read more.
Light-dependent protochlorophyllide oxidoreductase (LPOR, E.C.1.3.1.33) plays a crucial role in the biosynthesis of chlorophyll in plants. Therefore, inactivating LPOR can hinder the production of chlorophyll to achieve the effect of weed control. In this research, utilizing an active substructure splicing method, 20 new 1,2,4-oxadiazole compounds targeting LPOR were synthesized. Among them, compounds 5j, 5k and 5q exhibited superior inhibitory efficacy in greenhouse herbicidal trials. In vitro enzyme activity assays indicated that 5q significantly inhibited Arabidopsis thaliana LPOR (AtLPOR), with an IC50 value of 17.63 μM. Furthermore, compound 5q exhibited superior crop safety and holds potential application prospects for weed management in cotton. Molecular docking and dynamic simulations were employed to elucidate the binding mode and molecular mechanism of 5q with AtLPOR. These experimental and theoretical results indicate that 5q is a promising candidate for the development of novel herbicides targeting LPOR. Full article
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16 pages, 2669 KB  
Article
YOLOv7 for Weed Detection in Cotton Fields Using UAV Imagery
by Anindita Das, Yong Yang and Vinitha Hannah Subburaj
AgriEngineering 2025, 7(10), 313; https://doi.org/10.3390/agriengineering7100313 - 23 Sep 2025
Cited by 10 | Viewed by 2699
Abstract
Weed detection is critical for precision agriculture, enabling targeted herbicide application to reduce costs and enhance crop health. This study utilized UAV-acquired RGB imagery from cotton fields to develop and evaluate deep learning models for weed detection. As sustainable resource management gains importance [...] Read more.
Weed detection is critical for precision agriculture, enabling targeted herbicide application to reduce costs and enhance crop health. This study utilized UAV-acquired RGB imagery from cotton fields to develop and evaluate deep learning models for weed detection. As sustainable resource management gains importance in rainfed agricultural systems, precise weed identification is essential to optimize yields and minimize herbicide use. However, distinguishing weeds from crops in complex field environments remains challenging due to their visual similarity. This research employed YOLOv7, YOLOv7-w6, and YOLOv7-x models to detect and classify weeds in cotton fields, using a dataset of 9249 images collected under real field conditions. To improve model performance, we enhanced the annotation process using LabelImg and Roboflow, ensuring accurate separation of weeds and cotton plants. Additionally, we fine-tuned key hyperparameters, including batch size, epochs, and input resolution, to optimize detection performance. YOLOv7, achieving the highest estimated accuracy at 83%, demonstrated superior weed detection sensitivity, particularly in cluttered field conditions, while YOLOv7-x with accuracy at 77% offered balanced performance across both cotton and weed classes. YOLOv7-w6 with accuracy at 63% faced difficulties in distinguishing features in shaded or cluttered soil regions. These findings highlight the potential of UAV-based deep learning approaches to support site-specific weed management in cotton fields, providing an efficient, environmentally friendly approach to weed management. Full article
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20 pages, 2970 KB  
Review
The Rise of Eleusine indica as Brazil’s Most Troublesome Weed
by Ricardo Alcántara-de la Cruz, Laryssa Barbosa Xavier da Silva, Hudson K. Takano, Lucas Heringer Barcellos Júnior and Kassio Ferreira Mendes
Agronomy 2025, 15(8), 1759; https://doi.org/10.3390/agronomy15081759 - 23 Jul 2025
Cited by 7 | Viewed by 4239
Abstract
Goosegrass (Eleusine indica) is a major weed in Brazilian soybean, corn, and cotton systems, infesting over 60% of grain-producing areas and potentially reducing yields by more than 50%. Its competitiveness is due to its rapid emergence, fast tillering, C4 metabolism, and [...] Read more.
Goosegrass (Eleusine indica) is a major weed in Brazilian soybean, corn, and cotton systems, infesting over 60% of grain-producing areas and potentially reducing yields by more than 50%. Its competitiveness is due to its rapid emergence, fast tillering, C4 metabolism, and adaptability to various environmental conditions. A critical challenge relates to its widespread resistance to multiple herbicide modes of action, notably glyphosate and acetyl-CoA carboxylate (ACCase) inhibitors. Resistance mechanisms include 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) target-site mutations, gene amplification, reduced translocation, glyphosate detoxification, and mainly ACCase target-site mutations. This literature review summarizes the current knowledge on herbicide resistance in goosegrass and its management in Brazil, with an emphasis on integrating chemical and non-chemical strategies. Mechanical and physical controls are effective in early or local infestations but must be combined with chemical methods for lasting control. Herbicides applied post-emergence of weeds, especially systemic ACCase inhibitors and glyphosate, remain important tools, although widespread resistance limits their effectiveness. Sequential applications and mixtures with contact herbicides such as glufosinate and protoporphyrinogen oxidase (PPO) inhibitors can improve control. Pre-emergence herbicides are effective when used before or immediately after planting, with adequate soil moisture being essential for their activation and effectiveness. Given the complexity of resistance mechanisms, chemical control alone is not enough. Integrated weed management programs, combining diverse herbicides, sequential treatments, and local resistance monitoring, are essential for sustainable goosegrass management. Full article
(This article belongs to the Section Weed Science and Weed Management)
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